Acting On Beta-galatose-glycoside Bond (e.g., Beta-galactosidase, Etc.) Patents (Class 435/207)
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Patent number: 8288143Abstract: A novel cold-active beta-galactosidase is enzyme specific for lactose. The enzyme is thus useful in e.g. the food industry for catalyzing at low temperatures the hydrolysis of lactose disaccharide into its constituent monosaccharides, glucose and galactose. A method produces the cold-active beta-galactosidase by recombinant DNA technology.Type: GrantFiled: February 9, 2010Date of Patent: October 16, 2012Assignee: Kobenhavns UniversitetInventors: Peter Stougaard, Mariane Schmidt
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Publication number: 20120252093Abstract: Promoter regions associated with the Yarrowia lipolytica esterase/lipase (EL1) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: E I Du Pont De Nemours and CompanyInventor: Quinn Qun Zhu
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Publication number: 20120252073Abstract: A method of decomposing stubble, which is crop residue left on the ground, and a composition for treating the stubble are disclosed. The method comprises treating the stubble with the composition comprising a) polysaccharide-degrading enzyme; b) wetting agent; and, optionally, c) water and allowing the treated stubble to remain on the ground and decompose.Type: ApplicationFiled: March 28, 2012Publication date: October 4, 2012Applicant: RHODIA OPERATIONSInventors: Jean-Christophe Castaing, Gary Groves, Ashwin Rao, Thomas E. Ruch
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Patent number: 8278066Abstract: The present invention is directed to fusion proteins that can be used to assay gene transfer and expression both in vitro and in vivo. The fusion proteins contain a reporter protein, e.g. a somatostatin receptor, fused to a second protein, which may be a protein fusion tag. Alternatively, a fusion protein may be fused to a leader sequence. A leader sequence may localize an expressed protein, e.g. localize a fusion protein to the cell membrane. The invention includes nucleic acids encoding the fusion proteins and methods of assaying for gene expression.Type: GrantFiled: September 6, 2011Date of Patent: October 2, 2012Assignee: Board of Regents, The University of Texas SystemInventor: Vikas Kundra
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Patent number: 8236520Abstract: The present invention relates to beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.Type: GrantFiled: November 22, 2010Date of Patent: August 7, 2012Assignee: Korea Research Institute of Bioscience and BiotechnologyInventors: Hyun Ah Kang, Jae Kap Jeong, Ohsuk Kwon, Doo-Byoung Oh, Seonghun Kim
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Publication number: 20120183993Abstract: The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment b) optionally washing; c) enzymatic hydrolysis; d) fermentation; and e) optionally recovery of a fermentation product; wherein in step c) an enzyme composition is used that has a temperature optimum of 55 degrees C. or more, the hydrolysis time is 40 hours or more and the temperature is 50 degrees C. or more.Type: ApplicationFiled: October 5, 2010Publication date: July 19, 2012Applicant: DSM IP ASSETS B.V.Inventors: Johannes Petrus Smits, Elisabeth Maria Gierveld, Fop Cornelis Van Der Hor
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Publication number: 20120183998Abstract: The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.Type: ApplicationFiled: October 18, 2010Publication date: July 19, 2012Applicant: Kao CorporationInventors: Akihito Kawahara, Hiroshi Kodama, Katsutoshi Ara
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Publication number: 20120135468Abstract: Disclosed is a novel ?-galactosidase. Specifically disclosed are a ?-galactosidase derived from Bacillus circulans and a gene for the ?-galactosidase. The ?-galactosidase can be used, for example, in the production of milk, dairy products, fermented dairy products, galacto-oligosaccharides or supplements for foods.Type: ApplicationFiled: April 23, 2010Publication date: May 31, 2012Applicant: AMANO ENZYME INC.Inventors: Toru Katase, Yukiko Hoshi, Miho Nagaya, Shotaro Yamaguchi, Masashi Minoda, Kazuhiro Nakanishi
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Patent number: 8168756Abstract: Methods, compositions and kits are disclosed directed at levetiracetam derivatives, immunogens, signal generating moieties, antibodies that bind levetiracetam and immunoassays for detection of levetiracetam.Type: GrantFiled: October 22, 2009Date of Patent: May 1, 2012Assignee: ARK Diagnostics, Inc.Inventors: Johnny Jose Valdez, Byung Sook Moon, Ki Chung, Alejandro A. Orozco
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Publication number: 20120094937Abstract: Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.Type: ApplicationFiled: December 20, 2011Publication date: April 19, 2012Applicant: Schering CorporationInventors: Daniel M. Gorman, Troy D. Randall, Albert Zlotnik
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Publication number: 20120058223Abstract: A novel cold-active beta-galactosidase is enzyme specific for lactose. The enzyme is thus useful in e.g. the food industry for catalyzing at low temperatures the hydrolysis of lactose disaccharide into its constituent monosaccharides, glucose and galactose. A method produces the cold-active beta-galactosidase by recombinant DNA technology.Type: ApplicationFiled: February 9, 2010Publication date: March 8, 2012Applicant: Kobenhavns UniversitetInventors: Peter Stougaard, Mariane Schmidt
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Publication number: 20120040345Abstract: This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided.Type: ApplicationFiled: August 10, 2011Publication date: February 16, 2012Applicant: ALERE SAN DIEGO, INC.Inventors: Olaf Piepenburg, Derek L. Stemple, Niall A. Armes
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Patent number: 8101373Abstract: Truncated fragments of the small fragment of ?-galactosidase are provided that have low affinity for the large fragment of ?-galactosidase and provide for robust signals when two fusion proteins are complexed due to the binding of the proteins to which the ?-galactosidase fragments are fused. The truncated fragments do not interfere with the complexing of the two proteins and allow for the two proteins to function and be responsive to candidate compounds that affect complex formation.Type: GrantFiled: October 10, 2008Date of Patent: January 24, 2012Assignee: Discoverx CorporationInventors: Thomas Scott Wehrman, Keith R. Olson
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Publication number: 20120003715Abstract: A method for expressing and industrial scale production of recombinant lysosomal enzymes utilizing insect larva including the steps of infecting an insect larva population of the species Spodoptera littoralis via a recombinant baculovirus liquid suspension which permits the expression of at least one gene coding for a protein of interest, at least one of these genes coding for a lysosomal enzyme expressed in the insect larva, collecting of the previously infected insect larva expressing in a sufficient significant quantity the protein of interest and recovering the protein of interest from the collected insect larva.Type: ApplicationFiled: March 13, 2009Publication date: January 5, 2012Applicant: Bioorganic Research and Services S.L.Inventors: Victor Infante Viñolo, Rosa Maria Osuna Aguilar, Juan Jose Infante Viñolo
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Publication number: 20110265195Abstract: This invention provides an isolated peptide encoded by a nucleic acid which is at least 30 nucleotides in length and has a sequence which uniquely defines a herpesvirus associated with Kaposis' sarcoma, which herpesvirus is present in and recoverable from the HBL-6 cell line (ATCC Accession No. CRL 11762).Type: ApplicationFiled: April 26, 2011Publication date: October 27, 2011Inventors: Yuan Chang, Patrick S. Moore
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Patent number: 8013114Abstract: The present invention is directed to fusion proteins that can be used to assay gene transfer and expression both in vitro and in vivo. The fusion proteins contain a reporter protein, e.g. a somatostatin receptor, fused to a second protein, which may be a protein fusion tag. Alternatively, a fusion protein may be fused to a leader sequence. A leader sequence may localize an expressed protein, e.g localize a fusion protein to the cell membrane. The invention includes nucleic acids encoding the fusion proteins and methods of assaying for gene expression.Type: GrantFiled: December 13, 2006Date of Patent: September 6, 2011Assignee: The Board of Regents of the University of Texas SystemInventor: Vikas Kundra
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Publication number: 20110212221Abstract: The present invention relates to a lactase solution comprising a lactase solution comprising less than 10 g/kg of poly and oligosaccharides, a process for the production of such a lactase solution from an untreated lactase solution, a sterilized lactase, whereby such lactose is filter sterilized in-line with the milk production process.Type: ApplicationFiled: May 5, 2011Publication date: September 1, 2011Applicant: DSM IP ASSETS B.V.Inventors: Van Rudolf Fanciscus Wilhelmus Cornelis Beckhoven, Van Petrus Andreas Paridon
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Publication number: 20110212505Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl3, and the corresponding BGL3 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL3, recombinant BGL3 proteins and methods for producing the same.Type: ApplicationFiled: May 6, 2011Publication date: September 1, 2011Applicant: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Publication number: 20110207676Abstract: Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.Type: ApplicationFiled: April 27, 2011Publication date: August 25, 2011Inventors: Nico Luc Marc Callewaert, Wouter Vervecken, Karen Jacqueline Marcel De Pourcq, Steven Christian Jozef Geysens, Mouna Guerfal
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Publication number: 20110189733Abstract: A low cell density fermentation process for the production of heterologous proteins in microorganisms. The cell culture obtained by cultivating host microorganisms transformed with a vector carrying genetic material for the said proteins and an inducible promoter under batch fermentation conditions is fed with a feed medium after an OD600 of 0.16 to 8 has been achieved or after 0 to 4 hrs from the start of the fermentation process. The feed medium comprises 5 to 30% of carbon source and 1 to 30% of nitrogen source and 0 mg to 400 mg antibiotics and 2.5 to 4.25% inorganic phosphates and trace elements. The concentration of the carbon source in the feed medium is 10 to 30 and the amino acid content in nitrogen source is 45 to 95%. The initial feed rate is in the range of 0 ml/hr to 12 ml/hr and is raised exponentially by an exponent in the range of 0.1 to 0.4 and/or linearly with the slope of the curve in the range of 0.5 to 3. The production of the heterologous proteins is induced with 0.Type: ApplicationFiled: February 7, 2011Publication date: August 4, 2011Applicant: USV LimitedInventors: Milind Prabhakar NIPHADKAR, Genevieve Suzie Nazareth, Neelesh Ramesh Surlikar, Monsur Ahmed Borbhuiya, Uma Sankararaman, Dipanwita Maiti, Laxmi Srinivas Rao, Saptarshi Paul, Shrikant Mishra
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Patent number: 7955831Abstract: The present invention relates to a lactase solution comprising a lactase solution comprising less than 10 g/kg of poly and oligosaccharides, a process for the production of such a lactase solution from an untreated lactase solution, a sterilized lactase solution and to a process for the production of milk containing sterilized lactase, whereby such lactose is filter sterilized in-line with the milk production process.Type: GrantFiled: April 3, 2002Date of Patent: June 7, 2011Assignee: DSM IP Assets B.V.Inventors: Rudolf Franciscus Wilhelmus Cornelis Van Beckhoven, Petrus Andreas Van Paridon
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Publication number: 20110041222Abstract: A nucleotide leader sequence 5?-UTR comprises elements favorable to gene expression, such as repeated CAA trinucleotide elements in combination with repeated CT dinucleotide elements.Type: ApplicationFiled: December 27, 2007Publication date: February 17, 2011Applicant: UNIVERSITA 'DEGLI STUDI DI UDINEInventors: Stefano Marchetti, Francesca De Amicis, Tamara Patti
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Publication number: 20110003362Abstract: Chemically reactive carbocyanine dyes that are intramolecularly crosslinked between the 1-position and 3?-position, their bioconjugates and their uses are described. 1,3?-crosslinked carbocyanines are superior to those of conjugates of spectrally similar 1,1?-crosslinked or non-crosslinked dyes. The invention includes derivative compounds having one or more benzo nitrogens.Type: ApplicationFiled: July 12, 2010Publication date: January 6, 2011Inventors: Zhenjun Diwu, Jianheng Zhang, Yi Tang, Xiang Guobing
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Patent number: 7838276Abstract: Carbohydrate utilization-related and multidrug transporter nucleic acids and polypeptides, and fragments and variants thereof, are disclosed in the current invention. In addition, carbohydrate utilization-related and multidrug transporter fusion proteins, antigenic peptides, and anti-carbohydrate utilization-related and anti-multidrug transporter antibodies are encompassed. The invention also provides vectors containing a nucleic acid of the invention and cells into which the vector has been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed.Type: GrantFiled: October 22, 2008Date of Patent: November 23, 2010Assignee: North Carolina State UniversityInventors: Todd R. Klaenhammer, Eric R. Altermann, Rodolphe Barrangou, W. Michael Russell, Tri Duong
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Publication number: 20100266624Abstract: New styles of hepatitis C virus (HCV), referred to as HCV-3 and HCV-4, have been identified and sequenced. Antigenic regions of HCV-2, HCV-3 and HCV-4 polypeptides have been identified. Immunoassays for HCV and antibodies thereto are described, which allow more complete screening of blood samples for HCV, and allow HCV genotyping.Type: ApplicationFiled: March 19, 2010Publication date: October 21, 2010Applicant: Common Services AgencyInventors: Peter Simmonds, Shiu-Wan Chan, Peng Lee Yap
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Publication number: 20100260465Abstract: Isolated and/or recombinant enzymes that include surface binding domains, surfaces with active enzymes bound to them and methods of coupling enzymes to surfaces are provided. Enzymes can include large and/or multiple surface coupling domains for surface coupling.Type: ApplicationFiled: December 21, 2006Publication date: October 14, 2010Applicant: Pacific Biosciences of California, Inc.Inventors: David Hanzel, Jonas Korlach, Paul Peluso, Geoff Otto, Thang Pham, David Rank, Stephen Turner
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Publication number: 20100173427Abstract: Methods, compositions and kits are disclosed directed at levetiracetam derivatives, immunogens, signal generating moieties, antibodies that bind levetiracetam and immunoassays for detection of levetiracetam.Type: ApplicationFiled: October 22, 2009Publication date: July 8, 2010Inventors: Johnny Jose Valdez, Byung Sook Moon, Ki Chung, Alejandro A. Orozco
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Publication number: 20100129374Abstract: The purification and isolation of various genes which encode mammalian cell surface polypeptides. Nucleic acids, proteins, antibodies, and other reagents useful in modulating development of cells, e.g., lymphoid and myeloid, are provided, along with methods for their use.Type: ApplicationFiled: January 25, 2010Publication date: May 27, 2010Applicant: SCHERING CORPORATIONInventors: Alexander B.H. Bakker, Joseph H. Phillips, Lewis L. Lanier
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Publication number: 20100120091Abstract: The present invention relates to novel subtilase variants exhibiting improvements relative to the parent subtilase in one or more properties including: wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.Type: ApplicationFiled: January 15, 2010Publication date: May 13, 2010Applicant: NOVOZYMES A/SInventors: HENRIETTE DRABORG, VIBEKE SOKVGAARD NIELSEN, STEFAN MINNING
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Publication number: 20100055731Abstract: The invention relates to a fusion DNA construct comprising a KEX2 region comprising a KEX2 site and a KEX2 site pre-sequence immediately 5? to the KEX2 site, a fusion polypeptide, vectors and cells comprising the fusion DNA construct, methods for producing desired proteins from filamentous fungal cells and methods for enhancing the secretion and/or cleavage of a desired protein from a cell.Type: ApplicationFiled: June 21, 2007Publication date: March 4, 2010Inventors: Huaming Wang, Michael Ward
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Publication number: 20100047870Abstract: A low cell density fermentation process for the production of heterologous proteins in microorganisms. The cell culture obtained by cultivating host microorganisms transformed with a vector carrying genetic material for the said proteins and an inducible promoter under batch fermentation conditions is fed with a feed medium after an OD600 of 0.16 to 8 has been achieved or after 0 to 4 hrs from the start of the fermentation process. The feed medium comprises 5 to 30% of carbon source and 1 to 30% of nitrogen source and 0 mg to 400 mg antibiotics and 2.5 to 4.25% inorganic phosphates and trace elements. The concentration of the carbon source in the feed medium is 10 to 30 and the amino acid content in nitrogen source is 45 to 95%. The initial feed rate is in the range of 0 ml/hr to 12 ml/hr and is raised exponentially by an exponent in the range of 0.1 to 0.4 and/or linearly with the slope of the curve in the range of 0.5 to 3. The production of the heterologous proteins is induced with 0.Type: ApplicationFiled: December 21, 2004Publication date: February 25, 2010Applicant: USV LimitedInventors: Milind Prabhakar Niphadkar, Genevieve Suzle Nazareth, Neelesh Ramesh Surlikar, Monsur Ahmed Borbhuiya, Uma Sankararaman, Dipanwita Maiti, Laxmi Srinivas Rao, Saptarshi Paul, Shrikant Mishra
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Patent number: 7582426Abstract: The present invention relates to a method for display of proteinson spore surface and a method for improving protein with rapidity using the same, which comprises the steps of (i) preparing a vector for spore surface display comprising a gene construct containing a gene encoding spore coat protein and a gene encoding a protein of interest, wherein, when expressed, the gene construct expresses a fusion protein between the spore coat protein and the protein of interest, (ii) transforming a host cell with the vector for spore surface display; (iii) displaying the protein of interest on a surface of a spore of the host cell; and (iv) recovering the spore displaying on its surface the protein of interest.Type: GrantFiled: December 7, 2001Date of Patent: September 1, 2009Assignee: Geneofocus Co., Ltd.Inventors: June-Hyung Kim, Byung-Gee Kim, Soo-Keun Choi, Heung-Chae Jung, Jae-gu Pan
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Publication number: 20090215680Abstract: The invention concerns peptides useful as MAP kinase/ERK pathway-specific inhibitors relative to a given substrate in a given subcellular compartment.Type: ApplicationFiled: February 16, 2006Publication date: August 27, 2009Applicants: Universite Pierre Et Marie Curie, Centre National De La Recherche Scientifique - CNRSInventors: Jocelyne Caboche, Peter Vanhoutte
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Publication number: 20090209009Abstract: A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one ?-glucosidase enzyme and a binding agent for binding the ?-glucosidase enzyme to fiber solids present in the aqueous slurry. During the hydrolysis, both the cellulase enzyme and ?-glucosidase enzyme bind to the fiber solids. The hydrolysis is performed in a solids-retaining hydrolysis reactor so that unhydrolyzed fiber solids and bound enzyme are retained in the reactor longer than the aqueous phase of the slurry.Type: ApplicationFiled: June 22, 2007Publication date: August 20, 2009Applicant: IOGEN ENERGY CORPORATIONInventors: Jeffrey Tolan, Theresa White, John Tomashek
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Publication number: 20090117080Abstract: The present invention concerns a new ?-galactosidase with transgalactosylating activity isolated from Bifidobacterium bifidum. The ?-galactosidase is capable of converting lactose to a mixture of galactooligosaccharides which are ?-linked and unexpectedly produces the ?-linked disaccharide galactobiose. The mixture may be incorporated into numerous food products or animal feeds for improving gut health by promoting the growth of bifidobacteria in the gut, and repressing the growth of the pathogenic microflora.Type: ApplicationFiled: March 27, 2007Publication date: May 7, 2009Inventors: Georgios Tzortzis, Athanasios K. Goulas, Theodoros Goulas
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Patent number: 7527947Abstract: The present invention relates to a method for producing a polypeptide comprising using a signal peptide, to nucleic acid constructs comprising a first nucleotide sequence encoding the signal peptide and a second nucleotide sequence encoding a polypeptide which is foreign to the first nucleotide sequence. Furthermore, it also relates to expression vectors and host cells comprising the nuclei acid construct.Type: GrantFiled: June 14, 2005Date of Patent: May 5, 2009Assignee: Novozymes A/SInventors: Tomoko Matsui, Henriette Draborg, Steffen Danielsen
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Publication number: 20090110770Abstract: The present invention concerns a ?-galactosidase with transgalactosylating activity isolated from Bifidobacterium bifidum. The ?-galactosidase is capable of converting lactose to a mixture of oligosaccharides which are ?-linked and unexpectedly produces the ?-linked disaccharide galactobiose. The mixture may be incorporated into numerous food products or animal feeds for improving gut health by promoting the growth of bifidobacteria in the gut, and repressing the growth of the pathogenic microflora.Type: ApplicationFiled: January 23, 2007Publication date: April 30, 2009Inventors: Georgios Tzortzis, Athanasios K. Goulas, Theodoros Goulas
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Publication number: 20090098588Abstract: Truncated fragments of the small fragment of ?-galactosidase are provided that have low affinity for the large fragment of ?-galactosidase and provide for robust signals when two fusion proteins are complexed due to the binding of the proteins to which the ?-galactosidase fragments are fused. The truncated fragments do not interfere with the complexing of the two proteins and allow for the two proteins to function and be responsive to candidate compounds that affect complex formation.Type: ApplicationFiled: October 10, 2008Publication date: April 16, 2009Applicant: DiscoveRx CorporationInventors: Thomas Scott Wehrman, Keith R. Olson
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Publication number: 20080317669Abstract: The present invention relates to a nucleic acid encoding a polypeptide capable of binding to IgE from subjects allergic to venom of an insect from the order Hymenoptera having a homology of more than 70% to the amino acid sequence of SEQ ID NO: 2, which is the honey bee allergen C (Api m 5). The invention further relates to expression vectors, host cells and polypeptides encoded by the nucleic acid, as well as diagnostic and pharmaceutical uses thereof.Type: ApplicationFiled: June 26, 2007Publication date: December 25, 2008Applicant: PLS-Design GmbHInventors: Simon Blank, Benjamin Bockisch, Thomas Grunwald
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Publication number: 20080280818Abstract: The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries.Type: ApplicationFiled: July 23, 2007Publication date: November 13, 2008Applicant: Neose Technologies, Inc.Inventor: Shawn DeFrees
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Publication number: 20080274958Abstract: The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries.Type: ApplicationFiled: July 23, 2007Publication date: November 6, 2008Applicant: Neose Technologies, Inc.Inventor: Shawn DeFrees
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Publication number: 20080255040Abstract: The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries.Type: ApplicationFiled: July 23, 2007Publication date: October 16, 2008Applicant: Neose Technologies, Inc.Inventor: Shawn DeFrees
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Publication number: 20080248019Abstract: The present invention relates to a novel beta-galactosidase derived from Streptococcus pneumoniae, a BgaC protein exhibiting the enzyme activity, and a method for using the same. The protein can be used in the modification and analysis of sugar chain and used as an anti-cancer agent.Type: ApplicationFiled: June 25, 2007Publication date: October 9, 2008Applicant: Korea Research Institute of Bioscience and BiotechnologyInventors: Hyun Ah Kang, Jae Kap Jeong, Ohsuk Kwon, Doo-Byoung Oh, Seonghun Kim
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Publication number: 20080248959Abstract: The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries.Type: ApplicationFiled: July 23, 2007Publication date: October 9, 2008Applicant: Neose Technologies, Inc.Inventor: Shawn DeFrees
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Publication number: 20080242607Abstract: The present invention provides sequon polypeptides with an amino acid sequence including one or more exogenous O-linked glycosylation sequence of the invention. In addition, the present invention provides methods of making polypeptide conjugates as well as methods of using such conjugates and their pharmaceutical compositions. The invention further provides libraries of sequon polypeptides, wherein each member of such library includes at least one exogenous O-linked glycosylation sequence of the invention. Also provided are methods of making and using such libraries.Type: ApplicationFiled: July 23, 2007Publication date: October 2, 2008Applicant: Neose Technologies, Inc.Inventor: Shawn DeFrees
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Publication number: 20080171035Abstract: The purification and isolation of various genes which encode mammalian cell surface polypeptides. Nucleic acids, proteins, antibodies, and other reagents useful in modulating development of cells, e.g., lymphoid and myeloid, are provided, along with methods for their use.Type: ApplicationFiled: January 25, 2008Publication date: July 17, 2008Applicant: SCHERING CORPORATIONInventors: Alexander B.H. Bakker, Joseph H. Phillips, Lewis L. Lanier
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Patent number: 7393672Abstract: A full-length cDNA is obtained by analyzing the partial amino acid sequence of purified endo-?-galactosidase C, constructing primers based thereon, effecting PCR with the use of the genomic DNA of Clostridium perfringens as a template to thereby obtain a fragment of cDNA encoding endo-?-galactosidase, effecting cassette PCR by using the cDNA fragment thus obtained to thereby obtain the 5?-terminal and 3?-terminal domains, and further effecting PCR with the use of primers constructed on the basis of the data acquired above.Type: GrantFiled: April 20, 2006Date of Patent: July 1, 2008Assignee: Seikagaku CorporationInventors: Takashi Muramatsu, Haruko Ogawa, Hisako Muramatsu, Takaaki Kobayashi, Itsuo Yokoyama
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Patent number: 7354733Abstract: We disclose methods of sorting or separating mixtures of living cells (e.g., eukaryotic, prokaryotic, mammalian, pathogenic, bacterial, viral, etc.). We perform our methods by activating cell-selective photophoric labels, which photosensitize and chemically reduce a photosensitive metal compound to form metal grains, particles or crystals. The metal adheres to the cells and forms the basis for sorting or separating different cell types. Photophoric labels may include chemiluminescent agents such as peroxidase enzymes activated with peroxidase substrates capable of luminescence. Photosensitive metal compounds may be present in a light-sensitive matrix or emulsion containing photosensitizable metal compounds, which form metal grains, particles or crystals upon exposure to a developer solution. Developer solutions are formulated to substantially allow living cells to remain viable after exposure to the developing solution.Type: GrantFiled: March 26, 2002Date of Patent: April 8, 2008Assignee: Cellect Technologies Corp.Inventors: Shmuel Bukshpan, Gleb Zilberstein
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Publication number: 20080014188Abstract: This invention provides compositions of highly phosphorylated lysosomal enzymes, their pharmaceutical compositions, methods of producing and purifying such compounds and compositions and their use in the diagnosis, prophylaxis, or treatment of diseases and conditions, including particularly lysosomal storage diseases.Type: ApplicationFiled: February 7, 2005Publication date: January 17, 2008Inventors: Todd C. Zankel, Christopher M. Starr
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Patent number: 7291474Abstract: Disclosed are compounds of the general formulas: wherein Y is a moiety capable of being cleaved by a hydrolytic enzyme; L is a detectable label; X is a linking group; Z is a halogen; and R is hydrogen, an alkyl, or a halogen. Compounds of this general formula can be used as substrates in an analyte-dependent enzyme activation system, such as that employed in connection with catalyzed reporter depositions. Also disclosed are assays using the compounds, and kits for carrying out the assays.Type: GrantFiled: June 10, 2004Date of Patent: November 6, 2007Assignee: PerkinElmer LAS, Inc.Inventor: Mark N. Bobrow