Acting On Alpha-1, 6-glucosidic Bond (e.g., Isoamylase, Pullulanase, Etc.) Patents (Class 435/210)
-
Patent number: 7045332Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.Type: GrantFiled: December 18, 2001Date of Patent: May 16, 2006Assignee: Genencor International, Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
-
Patent number: 7026152Abstract: The invention provides compositions and methods for the synergistic degradation of oligosaccharides by endoglucanases. The invention further provides recombinant host cells containing one or more genes encoding endoglucanses which are capable of the synergistic degradation of oligosaccharides. Preferred host cells of the invention are ethanologenic and capable of carrying out simultaneous saccharification and fermentation resulting in the production of ethanol from complex cellulose substrates.Type: GrantFiled: June 19, 2001Date of Patent: April 11, 2006Assignee: University of Florida Research Foundation, Inc.Inventors: Lonnie O'Neal Ingram, Shengde Zhou
-
Patent number: 6939704Abstract: The present invention relates to increasing the production of a protein of interest from a fugal host. The invention discloses nucleotide sequences comprising, a regulatory region in operative association with xylanase secretion sequence and a gene of interest. The gene of interest encodes a protein selected from a pharmaceutical, nutraceutical, industrial, animal feed, food additive and an enzyme. Preferably, the gene of interest encodes a cellulase, hernicellulase, a lignin degrading enzyme, pectinase, protease, or peroxidase. The present invention also relates to vectors and hosts comprising these nucleic acid sequences, and to methods for the production of a protein of interest.Type: GrantFiled: September 9, 1999Date of Patent: September 6, 2005Assignee: Iogen Energy CorporationInventors: Theresa C. White, Sylvia McHugh, Christopher D. Hindle
-
Patent number: 6919428Abstract: Fusion protein comprising a cellulose binding domain and a domain having a high binding affinity for another ligand and detergent compositions comprising such fusion proteins.Type: GrantFiled: December 20, 2000Date of Patent: July 19, 2005Assignee: Unilever Home Products and Care USA division of Conopco, Inc.Inventors: Paul James Davis, Neil James Parry
-
Patent number: 6897358Abstract: The invention relates to nucleic acid molecules which code for enzymes and which are involved in the synthesis of starch in plants. These enzymes concern isoamylases derived from wheat. The invention also relates to vectors and host cells which contain the described nucleic acid molecules, especially transformed plant cells and plants which can be regenerated therefrom, which exhibit an increased or reduced activity of the inventive isoamylases.Type: GrantFiled: September 10, 2002Date of Patent: May 24, 2005Assignee: Bayer CropScience GmbHInventors: Horst Loerz, Stephanie Luetticke, Gernot Abel, Ulrich Genschel
-
Patent number: 6887986Abstract: The invention relates to a variant of a parent Termamyl-like ?-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an ?-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an ?-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an ?-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an ?-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like ?-amylase, which variant exhibits increased.Type: GrantFiled: November 16, 1999Date of Patent: May 3, 2005Assignee: Novozymes A/SInventors: Allan Svendsen, Soren Kjaerulff, Henrik Bisgard-Frantzen, Carsten Andersen
-
Patent number: 6849439Abstract: Barley ?-glucosidase is an important enzyme in the conversion of barley starch to fermentable sugars during the industrial production of ethanol, as in brewing and fuel ethanol production. The enzyme is, however, relatively thermolabile, a disadvantage for an enzyme useful in industrial processes which are preferably conducted at elevated temperatures. Site directed mutagenesis has been conducted to make mutant forms of barley ?-glucosidase which have improved thermostability. The sites for this site-directed mutagenesis were selected by sequence comparisons with the sequences of other ?-glucosidase proteins which are more thermostable. The recombinant mutant enzymes thus produced have been demonstrated to improve the thermostability of the enzyme.Type: GrantFiled: January 10, 2002Date of Patent: February 1, 2005Assignees: Wisconsin Alumni Research Foundation, The United States of America as represented by the Secretary of AgricultureInventors: Cynthia A. Henson, Elizabeth H. Muslin, Suzanne E. Clark
-
Patent number: 6846656Abstract: The present invention provides a process for economically producing N-acetylneuraminic acid without using expensive materials such as pyruvic acid and phosphoenolpyruvic acid. The process comprises: allowing (i) a culture of a microorganism having N-acetylneuraminic acid aldolase activity or N-acetylneuraminic acid synthetase activity, or a treated matter of the culture, (ii) a culture of a microorganism capable of producing pyruvic acid or a treated matter of the culture, or a culture of a microorganism capable of producing phosphoenolpyruvic acid or a treated matter of the culture, (iii) N-acetylmannosamine, and (iv) an energy source which is necessary for the formation of pyruvic acid or phosphoenolpyruvic acid to be present in an aqueous medium to form and accumulate N-acetylneuraminic acid in the aqueous medium; and recovering N-acetylneuraminic acid from the aqueous medium.Type: GrantFiled: August 25, 2000Date of Patent: January 25, 2005Assignee: Kyowa Hakko Kogyo Co., Ltd.Inventors: Satoshi Koizumi, Kazuhiko Tabata, Tetsuo Endo, Akio Ozaki
-
Patent number: 6815192Abstract: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.Type: GrantFiled: February 16, 2001Date of Patent: November 9, 2004Assignee: Novozymes A/SInventors: Kirk Schnorr, Per Lina Jørgensen, Martin Schulein
-
Publication number: 20040171023Abstract: This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.Type: ApplicationFiled: August 25, 2003Publication date: September 2, 2004Inventors: Perry G. Caimi, Mario W. Chen, Vasantha Nigarajan, Jean-Francois Tomb, Siqun Wang, Yuying Zhang
-
Publication number: 20040161837Abstract: The invention provides methods of purifying lysosomal proteins, pharmaceutical compositions for use in enzyme replacement therapy, and methods of treating Pompe's disease using purified human acid alpha glucosidase.Type: ApplicationFiled: February 13, 2004Publication date: August 19, 2004Inventors: Arnold J. Reuser, Ans T. van der Ploeg
-
Patent number: 6737563Abstract: The invention provides DNA constructs and genetically engineered seeds for the expression of amylopullulanase in plant seeds such as rice seeds. Related methods are also provided for the production of sugars, modified starches, and high protein products, and use of the glutelin promoter in the methods.Type: GrantFiled: January 16, 2002Date of Patent: May 18, 2004Assignee: Academia SinicaInventors: Su-May Yu, Jei-Fu Shaw
-
Publication number: 20040082028Abstract: The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostabilty, altered substrate specificity, increased specific activity or altered cleavage pattern. The present invention also relates to methods of making pullulanase variants having at least one altered property based on the three-dimensional structure of a parent pullulanase.Type: ApplicationFiled: November 16, 2001Publication date: April 29, 2004Applicant: Novozymes A/SInventor: Allan Svendsen
-
Patent number: 6716612Abstract: The invention describes DNA molecules which code for plant proteins having the biological activity of a debranching enzyme. Furthermore described are transgenic plant cells and plants having reduced or increased debranching enzyme activity, as well as modified starch isolatable from the cells and plants.Type: GrantFiled: July 29, 2002Date of Patent: April 6, 2004Inventors: Jens Kossmann, Michael Emmermann, Ivar Virgin, Andreas Renz
-
Publication number: 20040048247Abstract: The present invention relates to a method for producing a variant of a parent pullulanase, the variant having at least one altered property as compared to the parent pullulanase. The invention also relates to pullulanase variants and to the use of pullulanase variants of the invention for use in particular starch conversion processes.Type: ApplicationFiled: February 19, 2002Publication date: March 11, 2004Inventors: Allan Svendsen, Carsten Andersen, Torben Vedel Borchert
-
Patent number: 6689874Abstract: The present invention provides: a nucleotide sequence encoding alkaline pullulanase and exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity; a nucleotide sequence encoding alkaline &agr;-amylase possessing an amino acid sequence described in SEQ ID NO:3; a nucleotide sequence encoding alkaline pullulanase possessing an amino acid sequence described in SEQ ID NO:4; recombinant DNAs containing these nucleotide sequences, and transformed microorganisms harboring the recombinant DNAs. The technique of the present invention enables mass production of alkaline pullulanase exhibiting both alkaline &agr;-amylase and alkaline pullulanase activity.Type: GrantFiled: December 14, 2001Date of Patent: February 10, 2004Assignee: Kao CorporationInventors: Yuji Hatada, Kazuaki Igarashi, Katsuya Ozaki, Katsutoshi Ara, Shuji Kawai, Susumu Ito
-
Patent number: 6677137Abstract: Avian and reptile derived heparanase and nucleic acids encoding same.Type: GrantFiled: August 16, 2001Date of Patent: January 13, 2004Assignees: Insight Strategy & Marketing Ltd., Hadesit Medical Research Services and Development Ltd.Inventors: Orit Goldshmidt, Iris Pecker, Israel Vlodavsky, Israel Michal, Eyal Zcharia
-
Patent number: 6639126Abstract: The invention alters the physical characteristics of storage polyglucans including starch. Methods are provided to modify the polyglucan biosynthesis pathway by simultaneously altering the activity of a pullulanase debranching enzyme and the activity of another polypeptide in the polyglucan biosynthesis pathway. Compositions of the invention include transgenic plants and seeds having a modified polyglucan structure and/or content and elevated phytoglycogen levels. Additional compositions include a grain with increased energy availability for improved feed quality and industrial uses. Further compositions include a polyglucan with improved functional properties useful in a wide range of food and industrial applications.Type: GrantFiled: December 6, 2000Date of Patent: October 28, 2003Assignee: Pioneer Hi-Bred International, Inc.Inventors: Vincent J. H. Sewalt, George W. Singletary
-
Patent number: 6635454Abstract: The invention describes DNA molecules which code for plant proteins having the biological activity of a debranching enzyme. Furthermore described are transgenic plant cells and plants having reduced or increased debranching enzyme activity, as well as modified starch isolatable from the cells and plants.Type: GrantFiled: May 17, 2000Date of Patent: October 21, 2003Inventors: Jens Kossmann, Michael Emmermann, Ivar Virgin, Andreas Renz
-
Publication number: 20030190738Abstract: The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.Type: ApplicationFiled: February 26, 2003Publication date: October 9, 2003Applicant: Novozymes A/SInventors: Henrik Bisgard-Frantzen, Allan Svendsen
-
Patent number: 6600021Abstract: Methods of reducing cystine containing animal and plant proteins, and improving dough and baked goods' characteristics is provided which includes the steps of mixing dough ingredients with a thiol redox protein to form a dough and baking the dough to form a baked good. The method of the present invention preferably uses reduced thioredoxin with wheat flour which imparts a stronger dough and higher loaf volumes. Methods for reducing snake, bee and scorpion toxin proteins with a thiol redox (SH) agent and thereby inactivating the protein or detoxifying the protein in an individual are also provided. Protease inhibitors, including the, Kunitz and Bowman-Birk trypsin inhibitors of soybean, were also reduced by the NADP/thioredoxin system (NADPH, thioredoxin, and NADP-thioredoxin reductase) from either E. coli or wheat germ. When reduced by thioredoxin, the Kunitz and Bowman-Birk soybean trypsin inhibitors lose their ability to inhibit trypsin.Type: GrantFiled: November 23, 1999Date of Patent: July 29, 2003Assignee: The Regents of the University of CaliforniaInventors: Bob B. Buchanan, Karoly Kobrehel, Boihon C. Yee, Joshua H. Wong, Rosa Lozano, Jin-an Jiao, Sungho Shin
-
Patent number: 6566114Abstract: Novel mannanases comprising e.g. an amino acid sequence as shown in positions 31-330 of SEQ ID NO:2 or their homologues may be derived from e.g. Bacillus sp. I633, or may be encoded by polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO: 1 from nucleotide 91 to nucleotide 990, polynucleotide molecules that encode a polypeptide that is at least 65% identical to the amino acid sequence of SEQ ID NO: 2 from amino acid residue 31 to amino acid residue 330, or degenerate nucleotide sequences thereof. The mannanases are alkaline and are useful e.g. in cleaning compositions, in a fracturing fluid useful to fracture a subterranean formation, for modifying plant material, and for treatment of cellulosic fibers.Type: GrantFiled: June 24, 1999Date of Patent: May 20, 2003Assignee: Novozymes, A/SInventors: Markus Sakari Kauppinen, Martin Schülein, Kirk Schnorr, Lene Nonboe Andersen, Mads Eskelund Bjørnvad
-
Patent number: 6562600Abstract: The cyclic tetrasaccharide, cyclo{-6)-&agr;-D-Glcp-(1,3)-&agr;-D-Glcp-(1,6)-&agr;-D-Glcp-(1,3)-&agr;-D-Glcp-(1-}, may be produced by alternanase hydrolysis of complex carbohydrates other than alternan. Panose, pullulan, &agr;-D-Glcp-(1,6)-&agr;-D-Glcp-(1,3)-D-Glc, and D-glucans having alternating &agr;-(1,6) and &agr;-(1,4) linkages, are all hydrolyzed by alternanase to produce this cyclic tetrasaccharide. In this process, the cyclic tetrasaccharide is produced by contacting a solution of one or more of the above-mentioned complex carbohydrates with an amount of alternanase under conditions effective for activity of the enzyme. The substrate panose used in the reaction may be produced from a variety of polysaccharides or oligosaccharides, including starch, maltose, maltodextrins, pullulan, and mixtures thereof.Type: GrantFiled: June 25, 2001Date of Patent: May 13, 2003Assignee: The United States of America as represented by the Secretary of AgricultureInventor: Gregory L. Cote
-
Publication number: 20030054539Abstract: The present invention relates to enzyme preparations consisting essentially of an enzyme which has cellulytic activity and comprises a first amino acid sequence having the following sequence 1 (SEQ ID NO:79) Thr Arg Xaa Xaa Asp Cys Cys Xaa Xaa 1 2 3 4 5 6 7 8 9 Xaa Cys Xaa Trp Xaa 10 11 12 13 14Type: ApplicationFiled: December 10, 2001Publication date: March 20, 2003Applicant: Novozymes A/SInventors: Martin Schulein, Hanne Dela, Lene Nonboe Andersen, Soren Flensted Lassen, Markus Sakari Kauppinen, Lene Lange, Ruby Ilum Nielsen, Shinobu Takagi, Michiko Ihara
-
Patent number: 6534632Abstract: The present invention provides a protein defined in the following (A) or (B), and DNA coding for the same: (A) a protein which has the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4, or (B) a protein which has the amino acid sequence including substitution, deletion, insertion, or transition of one or several amino acid residues in SEQ ID NO: 2 or SEQ ID NO: 4, and exhibits activity to eliminate a sialic acid residue from a non-reducing terminal of ganglioside.Type: GrantFiled: March 28, 2001Date of Patent: March 18, 2003Inventors: Taeko Miyagi, Tadashi Wada, Yuko Yoshikawa
-
Publication number: 20030022348Abstract: The present invention relates to modified pullulanases useful in the starch industry. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for the saccharification of starch comprising the use of the enzymatic compositions.Type: ApplicationFiled: September 16, 2002Publication date: January 30, 2003Inventors: Brian S. Miller, Jayarama K. Shetty
-
Patent number: 6509183Abstract: A DNA fragment coding for alkaline pullulanase, which contains about 6.3 Kb base pairs and has a restriction map shown in FIG. 2, a recombinant vector containing the DNA fragment, and a microorganism carrying the vector. Also disclosed is a method of preparing alkaline pullulanase which comprises culturing a transformant microorganism which has been transformed with a recombinant vector containing a DNA fragment coding for alkaline pullulanase, having a restriction enzyme map shown in FIG. 2 and having about 6.3 Kb base pairs. According to the method of the invention, alkaline pullulanase which is useful as a component of detergents can be mass-produced in a low cost.Type: GrantFiled: September 28, 1994Date of Patent: January 21, 2003Assignee: Kao CorporationInventors: Katsutoshi Ara, Kazuaki Igarashi, Katsuhisa Saeki, Shuji Kawai, Susumu Ito
-
Publication number: 20030013180Abstract: The present invention relates to modified pullulanases useful in the starch industry. The present invention provides methods for producing the modified pullulanase, enzymatic compositions comprising the modified pullulanase, and methods for the saccharification of starch comprising the use of the enzymatic compositions.Type: ApplicationFiled: March 3, 1999Publication date: January 16, 2003Inventors: BRIAN S. MILLER, JAYARAMA K. SHETTY
-
Publication number: 20020194642Abstract: The subject invention pertains to novel mutant polynucleotide molecules that encode enzymes that have increased heat stability. These polynucleotides, when expressed in plants, result in increased yield in plants grown under conditions of heat stress. The polynucleotide molecules of the subject invention encode maize endosperm ADP glucose pyrophosphorylase (AGP) and soluble starch synthase (SSS) enzyme activities. Plants and plant tissue bred to contain, or transformed with, the mutant polynucleotides, and expressing the polypeptides encoded by the polynucleotides, are also contemplated by the present invention. The subject invention also concerns methods for isolating polynucleotides and polypeptides contemplated within the scope of the invention. Methods for increasing yield in plants grown under conditions of heat stress are also provided.Type: ApplicationFiled: February 19, 2002Publication date: December 19, 2002Inventors: L. Curtis Hannah, Thomas W. Greene
-
Publication number: 20020184662Abstract: Barley &agr;-glucosidase is an important enzyme in the conversion of barley starch to fermentable sugars during the industrial production of ethanol, as in brewing and fuel ethanol production. The enzyme is, however, relatively thermolabile, a disadvantage for an enzyme useful in industrial processes which are preferably conducted at elevated temperatures. Site directed mutagenesis has been conducted to make mutant forms of barley &agr;-glucosidase which have improved thermostability. The sites for this site-directed mutagenesis were selected by sequence comparisons with the sequences of other &agr;-glucosidase proteins which are more thermostable. The recombinant mutant enzymes thus produced have been demonstrated to improve the thermostability of the enzyme.Type: ApplicationFiled: January 10, 2002Publication date: December 5, 2002Inventors: Cynthia A. Henson, Elizabeth H. Muslin, Suzanne E. Clark
-
Patent number: 6485954Abstract: The present invention relates to an enzyme with galactanase activity, a DNA construct encoding the enzyme with galactanase activity, a method of producing the enzyme, an enzyme composition comprising said enzyme with galactanase activity, and the use of said enzyme and enzyme composition for a number of industrial applications.Type: GrantFiled: November 28, 2000Date of Patent: November 26, 2002Assignee: Novozymes A/SInventors: Lene Venke Kofod, Markus Sakari Kauppinen, Lene Nonboe Andersen, Ib Groth Clausen, Anette Müllertz
-
Patent number: 6469230Abstract: The invention relates to isolated nucleic acids obtainable from potato which encode isoamylases, a type of starch branching enzyme, particularly SEQ ID NOS:1-3 or variants encoding SEQ ID NOS:4-6; vectors comprising the nucleic acids; plant cells and plants transformed therewith; and methods for making plants which produce starches with modified branching characteristics.Type: GrantFiled: January 21, 2000Date of Patent: October 22, 2002Assignee: Plant Bioscience LimitedInventors: Elizabeth Anne Edwards, Alison Mary Smith, Catherine Rosemary Martin, Regla Bustos Guillen
-
Patent number: 6448049Abstract: The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.Type: GrantFiled: April 6, 2000Date of Patent: September 10, 2002Assignee: Novozymes AISInventors: Noriko Tsutsumi, Henrik Bisgård-Frantzen, Allan Svendsen
-
Patent number: 6433253Abstract: The present invention relates to DNA sequences which, on the codogenic strand, code plant debranching enzymes whose transcripts formed in transgenic plants code new proteins with the enzymatic activity of debranching enzymes which in transgenic plants reduce the degree of branching of amylopectin starch. The invention also relates to DNA sequences which on the codogenic strand code plant debranching enzymes whose transcripts formed in transgenic plants prevent the synthesis of proteins with the enzymatic activity of debranching enzymes, which in transgenic plants increases the degree of branching of amylopectin starch, and also to recombinant plasmids on which these DNA sequences are localized and which can be introduced into plant cells and plants.Type: GrantFiled: August 6, 1999Date of Patent: August 13, 2002Assignee: Hoechst Schering AgrEVO GmbHInventors: Jens Kossmann, Michael Emmermann, Ivar Virgin
-
Patent number: 6432689Abstract: The present invention provides thermophilic alkaliphilic bacteria designated Thermopallium natronophilum and thermophilic alkaliphilic polypeptides obtainable therefrom. It also provides compositions, particularly detergent compositions comprising the polypeptides.Type: GrantFiled: October 30, 2000Date of Patent: August 13, 2002Assignee: Genencor International, Inc.Inventors: Brian E. Jones, Margareta A. Herweijer, Michael J. Danson, David W. Hough, Carl R. Thompson
-
Patent number: 6429358Abstract: This invention relates to isolated nucleic acid fragments encoding all or a substantial portion of a corn pullulanase. The invention also relates to the construction of chimeric genes encoding all or a portion of a corn pullulanase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of corn pullulanase in a transformed host cell.Type: GrantFiled: November 5, 1999Date of Patent: August 6, 2002Assignee: E. I. du Pont de Nemours and CompanyInventor: Karen E. Broglie
-
Patent number: 6426208Abstract: The present invention provides a recombinant &agr;-L-iduronidase and biologically active fragments and mutants thereof, methods to produce and purify this enzyme as well as methods to treat certain genetic disorders including- &agr;-L-iduronidase deficiency and mucopolysaccharidosis I (MPS 1).Type: GrantFiled: November 12, 1999Date of Patent: July 30, 2002Assignee: Harbor-UCLA Research and Education InstituteInventors: Emil D. Kakkis, Becky Tanamachi
-
Publication number: 20020081670Abstract: The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.Type: ApplicationFiled: April 12, 2001Publication date: June 27, 2002Applicant: Novozymes A/SInventors: Henrik Bisgard-Frantzen, Allan Svendsen
-
Patent number: 6410235Abstract: The discriminating capability of hybridization assays is increased by a combination of labelled primers which produce amplificates of one strand of a nucleic acid with a capture probe which is complementary to the same strand of the nucleic acid.Type: GrantFiled: June 4, 1999Date of Patent: June 25, 2002Assignee: Roche Diagnostics GmbHInventors: Kurt Weindel, Joachim Brand
-
Publication number: 20020034798Abstract: A process is disclosed for preparing aqueous phytase-containing liquids involving culturing microorganisms of the genus Aspergillus or Trichoderma in a medium containing assimilable carbon and nitrogen sources (e.g., glucose and ammonium ions), filtering the medium, and subjecting the resulting filtrate to ultra-filtration to give an aqueous composition having at least 14,000 FTU/g. This aqueous liquid optimally can be used to prepare granulates that can be incorporated in animal feedstuffs.Type: ApplicationFiled: June 4, 1998Publication date: March 21, 2002Inventors: RUDOLF CAROLUS MARIA BARENDSE, GABRIEL MARINUS HENRICUS MEESTERS, CARL SIDONIUS MARIA ANDELA
-
Patent number: 6346244Abstract: The present invention relates to protein disulfide isomerases which are encoded by a nucleic acid sequence which hybridizes with (i) the DNA sequence of SEQ ID NO:1 or (ii) the DNA sequence of SEQ ID NO:2, under the following conditions: presoaking in 5×SSC and prehybridizing for 1 h at ˜40° C. in a solution of 5×SSC, 5×Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 &mgr;g of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 &mgr;Ci 32-P-dCTP labelled probe for 18 h at ˜40° C. followed by washing three times in 2×SSC, 0.2% SDS at 40° C. for 30 minutes; and fragments thereof. The present invention also relates to DNA sequences encoding the protein disulfide isomerases, compositions comprising said protein disulfide isomerases and methods of use thereof.Type: GrantFiled: March 4, 1999Date of Patent: February 12, 2002Assignee: Novozymes A/SInventor: Carsten Mailand Hjort
-
Publication number: 20020002713Abstract: This invention relates to an isolated nucleic acid fragment encoding a starch branching enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch branching enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch branching enzyme in a transformed host cell.Type: ApplicationFiled: February 23, 2001Publication date: January 3, 2002Inventors: Stephen M. Allen, Diane M. Beckles, Karlene H. Butler, Richard W. Pearlstein
-
Patent number: 6300115Abstract: Disclosed herein are DNA expression constructs containing an &agr;-amylase promoter sequence derived from Bacillus stearothermophilus, an &agr;-amylase leader sequence derived from Bacillus stearothermophilus, and a DNA sequence encoding a pullulanase derived from Bacillus naganoensis. Microbial hosts transformed to contain the expression constructs secret function pullulanases. Also disclosed is a process for making recombinant pullulanases utilizing the expression constructs and a recombinant pullulanase which can be produced in Bacillus subtilis.Type: GrantFiled: May 18, 1999Date of Patent: October 9, 2001Assignee: Enzyme Bio-Systems Ltd.Inventors: W. Martin Teague, Phillip J. Brumm, Larry N. Allen, Igor A. Brikun
-
Patent number: 6291229Abstract: The present invention provides novel aerobic, Gram-positive alkaliphilic bacteria which have been isolated from in and around alkaline soda lakes. These alkaliphiles have been analyzed according to the principles of numerical taxonomy with respect to each other and also to a collection of known bacteria. In addition, these bacterial taxa are further circumscribed by an analysis of the lipid components which serve as chemotaxonomic markers. The alkaliphiles of the present invention produce alkalitolerant enzymes which are capable of performing their functions at high pH which makes them uniquely suited for applications requiring such extreme conditions.Type: GrantFiled: January 11, 1999Date of Patent: September 18, 2001Assignee: Genencor International, Inc.Inventors: Brian Edward Jones, William Duncan Grant, Nadine Claire Collins
-
Patent number: 6265197Abstract: The invention relates to a genetically engineered variant of a parent starch debranching enzyme, i.e. a pullulanase or an isamylase, the enzyme variant having an improved thermostability at a pH in the range of 4-6 compared to the parent enzyme and/or an increased activity towards amylopectin and/or glycogen compared to the parent enzyme, to methods for producing such starch debranching enzyme variants with improved thermostability and/or altered substrate specificity, and to a method for converting starch to one or more sugars using at least one such enzyme variant.Type: GrantFiled: July 1, 1999Date of Patent: July 24, 2001Assignee: Novozymes A/S KrogshoejvejInventors: Henrik Bisgård-Frantzen, Allan Svendsen
-
Patent number: 6218164Abstract: The present invention provides thermophilic alkaliphilic bacteria designated Thermopallium natronophilum and thermophilic alkaliphilic polypeptides obtainable therefrom. It also provides compositions, particularly detergent compositions comprising the polypeptides.Type: GrantFiled: June 2, 1998Date of Patent: April 17, 2001Assignee: Genencor International, Inc.Inventors: Brian E. Jones, Margareta A. Herweijer, Michael J. Danson, David W. Hough, Carl R. Thompson
-
Patent number: 6197565Abstract: The invention relates to a variant of a parent Termamyl-like &agr;-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an &agr;-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an &agr;-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an &agr;-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an &agr;-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like &agr;-amylase, which variant exhibits increased.Type: GrantFiled: November 16, 1998Date of Patent: March 6, 2001Assignees: Novo-Nordisk A/S, Novo AlleInventors: Allan Svendsen, Sóeren Kjaerulff, Henrik Bisgaard-Frantzen, Carsten Andersen
-
Patent number: 6184001Abstract: Novel cyclodextrin glycosyl transferases (CGTase) can be produced by anaerobic cultivation of strains of Thermoanaerobacter or Thermoanaerobium. They are more thermostable than known CGTases and have temperature optimum about 95° C. The novel CGTases can be used for starch liquefaction at pH 4.5 and temperature exceeding 100° C. in the production of dextrose or ethanol. They can also be used for conversion of liquefied starch to cyclodextrin at a temperature of 80-90° C. A method for enzymatically converting solid and liquefied starch into cyclodextrin using cyclodextrin glycosyl transferases (CGTase) elaborated by thermophilic obligate anaerobic strains belonging to the genus Clostridium. These CGTases are characterized by thermostability and a capability to liquefy starch and/or to convert liquefied starch to cyclodextrin at pH 5.0-5.5 and 60-90° C.Type: GrantFiled: October 4, 1994Date of Patent: February 6, 2001Assignee: Novozymes A/SInventor: Robert L. Starnes
-
Patent number: 6117665Abstract: The invention describes DNA molecules which code for plant proteins having the biological activity of a debranching enzyme. Furthermore described are transgenic plant cells and plants having reduced or increased debranching enzyme activity, as well as modified starch isolatable from transgenic cells and plants.Type: GrantFiled: November 25, 1997Date of Patent: September 12, 2000Inventors: Jens Kossmann, Michael Emmermann, Ivar Virgin, Andreas Renz
-
Patent number: 6087149Abstract: The present invention relates to a starch conversion process of the type which includes a debranching step wherein an isoamylase being active at the process conditions prevailing is used for debranching the starch and to the use of thermostable isoamylases for starch conversion. The invention further relates to an isolated isoamylase obtained from a strain of the genus Rhodothermus and to cloned DNA sequences encoding isoamylases derived from a strain of Rhodothermus or Sulfolobus, to expression vectors comprising said DNA sequence, host cells comprising such expression vectors, and finally to methods for producing said isoamylases.Type: GrantFiled: August 4, 1998Date of Patent: July 11, 2000Assignee: Novo Nordisk A/SInventors: Noriko Tsutsumi, Henrik Bisg.ang.rd-Frantzen, Allan Svendsen