Trypsin; Chymotrypsin Patents (Class 435/213)
  • Publication number: 20150037842
    Abstract: The disclosure relates to a Gram negative bacterial cell that is transformed with a nucleic acid molecule that encodes a Gram positive twin-arginine translocase and including methods for the production of polypeptides.
    Type: Application
    Filed: February 22, 2013
    Publication date: February 5, 2015
    Inventor: Colin Robinson
  • Publication number: 20150023944
    Abstract: A method for treating a Parkinson's patient with digestive/pancreatic enzymes involves administering an effective amount of digestive/pancreatic enzymes to an individual having the disorder in order to improve a symptom of the disorder. In addition, a method is provided for determining whether an individual has, or may develop, Parkinson's disease or related dysautonomic disorders and for determining whether an individual will benefit from the administration of pancreatic/digestive enzymes to treat the dysautonomic disorder.
    Type: Application
    Filed: September 23, 2014
    Publication date: January 22, 2015
    Inventor: Joan M. Fallon
  • Patent number: 8927290
    Abstract: Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.
    Type: Grant
    Filed: February 16, 2011
    Date of Patent: January 6, 2015
    Assignee: One Lambda, Inc.
    Inventors: Adam Idica, Chun-Tsan Deng, Paul I. Terasaki
  • Patent number: 8920828
    Abstract: Devices for the treatment of intervertebral discs are described. The devices, when implanted into the nucleus pulposus of an intervertebral disc, provide for the controlled release of one or more active agents into the disc. The active agent can be a chemonucleolytic agent such as chymopapain. The device can also comprise one or more binders. The device can be an elongate solid body having a tapered or rounded insertion end. Alternatively, the device can include a plurality of particles. For devices containing multiple active agents, the configuration of the device be chosen to provide for the sequential or simultaneous release of each of the active agents. The elongate solid body can include a sheath comprising a first active agent and a core comprising a second active agent.
    Type: Grant
    Filed: November 29, 2006
    Date of Patent: December 30, 2014
    Assignee: Warsaw Orthopedic, Inc.
    Inventor: Hai H. Trieu
  • Publication number: 20140335594
    Abstract: The present invention relates to a method for selectively removing virus and/or virus DNA from a solution comprising a target protein, whereby acetone is used as a precipitation agent to precipitate virus and/or virus DNA as well as the target protein.
    Type: Application
    Filed: December 19, 2012
    Publication date: November 13, 2014
    Inventors: Poul Johansen, Cecilia Jansson Kepka
  • Publication number: 20140127307
    Abstract: The present invention relates to a small particle size composition comprising pancreatin containing digestive enzymes for use in patients in need, including pediatric, geriatric, and adult patients, particularly those patients with dysphagia or wherein enteral administration using such composition would be suitable. In addition, the invention is directed to the composition as particles, such as micropellets or microgranules having a high potency, high useable yield and at least 10%-90% of 400-800 ?m. Furthermore, the composition optionally has an improved enteric coating and concomitant improved stability and enzyme activity compared to conventional prepared enterically coated pancreatic enzyme particles.
    Type: Application
    Filed: November 2, 2012
    Publication date: May 8, 2014
    Applicant: APTALIS PHARMA LIMITED
    Inventors: Gopi M. VENKATESH, Craig Kramer, Flavio Fabiani, Luigi Mapelli, Giovanni Ortenzi, Massimo Latino
  • Publication number: 20140099696
    Abstract: Microspheres are produced by contacting a solution of a macromolecule or small molecule in a solvent with an antisolvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals, nutraceuticals, cosmetic products and the like of defined dimensions.
    Type: Application
    Filed: April 30, 2013
    Publication date: April 10, 2014
    Applicant: Ansun Biopharma, Inc.
    Inventors: Michael P. Malakhov, Fang Fang
  • Patent number: 8652849
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: October 14, 2008
    Date of Patent: February 18, 2014
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
  • Publication number: 20140017716
    Abstract: Compositions and methods are provided for the rapid and efficient denaturation and degradation of protein samples. The compositions and methods produce samples that can readily be analyzed by, for example, mass spectrometry. Unwanted dilution of the sample is avoided and the samples and methods are amenable for use with robotic laboratory sample handling instruments. The compositions and methods surprisingly provide significantly improved reproducibility and accuracy of the resulting mass spectrometric analyses.
    Type: Application
    Filed: July 11, 2013
    Publication date: January 16, 2014
    Inventor: N. Leigh ANDERSON
  • Publication number: 20130337277
    Abstract: A liquid composition for producing a self-destructible temporary structure by additive manufacturing is provided. The liquid composition includes a biosynthetic copolymer as a degradable component and an enzyme as a disintegrating agent, the disintegrating agent is capable of disintegrating the degradable component, wherein after exposure to an external trigger, the disintegrating agent gradually degrades the degradable component.
    Type: Application
    Filed: June 25, 2013
    Publication date: December 19, 2013
    Inventors: Daniel DIKOVSKY, Eduardo Napadensky
  • Publication number: 20130330758
    Abstract: At least at least one embodiment of the present invention relates to a method for using a high pressure-resistant enzyme in a high pressure condition; a method for promoting the activity of the high pressure-resistant enzyme by means of a high pressure treatment; a composition, which contains the high pressure-resistant enzyme, for decomposing proteins under a high pressure condition; a composition, which contains the composition for decomposing proteins, for preparing natural flavoring substances; a container for high pressure treatment, which contains the composition for decomposing proteins; and a method for measuring the activity of the high pressure-resistant enzyme, which comprises a step of decomposing an azocasein solution serving as a substrate by using the high pressure-resistant enzyme treated under a high pressure condition.
    Type: Application
    Filed: February 20, 2012
    Publication date: December 12, 2013
    Applicant: KOREA FOOD RESEARCH INSTITUTE
    Inventors: Nam-Soo Kim, Chong-Tai Kim, Yong-Jin Cho, Chul-Jin Kim, Jin-Soo Maeng, Soo-Jin Kwon
  • Publication number: 20130273203
    Abstract: The present invention relates to polypeptides having endopeptidase activity and to methods of producing and using the polypeptides. The invention also relates to methods of making a food protein hydrolysate using a trypsin-like endopeptidase derived from a bacterium.
    Type: Application
    Filed: September 30, 2011
    Publication date: October 17, 2013
    Applicant: NOVOZYMES A/S
    Inventors: Peter Rahbek Oestergaard, Carsten P. Sonksen, Tine Hoff, Gitte B. Lynglev
  • Publication number: 20130273606
    Abstract: The invention relates to a nucleic acid molecule encoding a multimeric precursor which after transcription is specifically cleaved in vivo to form multiple copies of a protein of interest. The invention further relates to a cell comprising this nucleic acid molecule and a method for producing a protein of interest using this cell.
    Type: Application
    Filed: April 18, 2013
    Publication date: October 17, 2013
    Inventors: Anna Cornelia Hendrika VAN HAUTUM, Arjo Lysander DE BOER, Jan Bastiaan BOUWSTRA, Peter Franciscus Theresius Maria VAN ASTEN
  • Publication number: 20130196910
    Abstract: The invention relates to truncated growth factors and variants thereof. The invention also relates to methods of making and using the truncated growth factors.
    Type: Application
    Filed: March 24, 2011
    Publication date: August 1, 2013
    Applicant: LIFENET HEALTH
    Inventors: Xiaofei Qin, Silvia Chen, Jingsong Chen, James Clagett
  • Publication number: 20130196909
    Abstract: The invention relates to truncated growth factors and variants thereof. The invention also relate to methods of making and using the truncated growth factors.
    Type: Application
    Filed: March 24, 2011
    Publication date: August 1, 2013
    Applicant: LIFENET HEALTH
    Inventors: Xiaofei Qin, Silvia Chen, Jingsong Chen, James Clagett
  • Patent number: 8486390
    Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: July 16, 2013
    Assignee: Curemark LLC
    Inventor: Joan M. Fallon
  • Publication number: 20130177940
    Abstract: The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaa1-Xaa2-His, wherein Xaa1 is L, Y or F and Xaa2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin.
    Type: Application
    Filed: January 14, 2013
    Publication date: July 11, 2013
    Inventor: Frank Bordusa
  • Publication number: 20130095551
    Abstract: A method for changing conformation of globular proteins is provided. The method controls the concentration of the globular proteins and the adsorption time of the globular proteins from the aqueous solution to the air/liquid interface, so that the main conformation of the globular proteins in a protein monolayer can be changed into ?-sheet or ?-helix. Meanwhile, the protein monolayer having the conformation of ?-sheet or ?-helix can be vertically deposited and transferred onto a substrate for various applications according to needs. The present invention can change three-dimensional structures of biological molecules and remain original functions thereof without additionally using any physical/chemical treatment to change the conformation of the globular proteins.
    Type: Application
    Filed: May 24, 2012
    Publication date: April 18, 2013
    Applicant: NATIONAL CHENG KUNG UNIVERSITY
    Inventors: Yuh-lang Lee, Ke-hsuan Wang
  • Publication number: 20130095152
    Abstract: A therapeutic composition for the treatment of the symptoms of complex regional pain syndrome and a method for preparing the therapeutic composition is disclosed. The therapeutic composition is a stable pharmaceutical composition comprising one or more digestive and/or pancreatic enzymes. The therapeutic composition may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic composition may be made orally, through injection, by adherence of a medicated patch or by other methods. Further, a method of using fecal chymotrypsin level as a biomarker for the presence of complex regional pain syndrome, or the likelihood of an individual to develop complex regional pain syndrome is disclosed.
    Type: Application
    Filed: December 5, 2012
    Publication date: April 18, 2013
    Applicant: CUREMARK, LLC
    Inventor: Curemark, LLC
  • Publication number: 20130090290
    Abstract: The invention relates to truncated growth factors and variants thereof. The invention also relates to methods of making and using the truncated growth factors.
    Type: Application
    Filed: March 21, 2011
    Publication date: April 11, 2013
    Applicant: LIFENET HEALTH
    Inventors: Xiaofei Qin, Silvia Chen, Jingsong Chen, James A. Clagett
  • Patent number: 8410048
    Abstract: The invention relates to a method for producing a type of insulin by genetically engineering a precursor thereof and converting said precursor to the respective insulin in an enzyme-catalyzed ligation reaction with lysine amide or arginine amide, or by lysine or arginine which is modified by protective groups, and optionally subsequent hydrolysis.
    Type: Grant
    Filed: January 7, 2009
    Date of Patent: April 2, 2013
    Assignee: Sanofi-Aventis Deutschland GmbH
    Inventors: Paul Habermann, Frank Zocher
  • Publication number: 20130078705
    Abstract: A protease for treating nail abnormalities is a type of protease. Since nails are mostly composed of keratin protein, the optimum protease for decomposing the main component (keratin) of the nail is keratinase or serine protease which is functionally similar to keratinase. The serine protease include trypsin, chymotrypsin, elastase, subtilisin, etc, these enzymes can be used to treat pathologically or mechanically damaged nail tissue to thin or remove keratin which is the key component of the nail, thus facilitating drug delivery in later treatment.
    Type: Application
    Filed: September 22, 2011
    Publication date: March 28, 2013
    Inventor: Hsuehkuan LU
  • Patent number: 8389261
    Abstract: The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
    Type: Grant
    Filed: December 28, 2009
    Date of Patent: March 5, 2013
    Assignee: Baxter Healthcare S.A.
    Inventors: Artur Mitterer, Christa Tauer, Manfred Reiter, Wolfgang Mundt
  • Patent number: 8383782
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: February 26, 2013
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
  • Publication number: 20130017546
    Abstract: Newly identified proteins as markers for the detection of breast tumors, or as therapeutic targets for treatment thereof; affinity ligands capable of selectively interacting with the newly identified markers, as well as methods for tumor diagnosis and therapy using such ligands.
    Type: Application
    Filed: October 26, 2010
    Publication date: January 17, 2013
    Applicant: Externautics S.P.A.
    Inventors: Renata Grifantini, Piero Pileri, Susanna Campagnoli, Alberto Grandi, Matteo Parri, Andrea Pierleoni, Renzo Nogarotto
  • Patent number: 8349580
    Abstract: A method for testing efficacy of a protease enzyme assay, the method comprising providing an enzyme which acts as a surrogate enzyme control for the protease enzyme; combining the surrogate enzyme control with an assay substrate for the protease enzyme; and determining a change in the assay substrate resulting from the surrogate enzyme control acting on the assay substrate; wherein the protease enzyme is selected from the group consisting of metalloproteinases, serine proteases, and cysteine proteases; a method for conducting a protease enzyme assay using the method for testing efficacy; a kit including an enzyme which acts as a surrogate enzyme control for a protease enzyme in testing efficacy of a protease enzyme assay; and a method of releasing the kit are provided.
    Type: Grant
    Filed: August 30, 2010
    Date of Patent: January 8, 2013
    Assignee: 3M Innovative Properties Company
    Inventors: Joseph J. Stoffel, Louis C. Haddad
  • Patent number: 8318158
    Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: November 27, 2012
    Assignee: Curemark, LLC
    Inventor: Joan M. Fallon
  • Patent number: 8211682
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: October 3, 2008
    Date of Patent: July 3, 2012
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
  • Patent number: 8206958
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: December 5, 2007
    Date of Patent: June 26, 2012
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Tapan K. Dey, Carlos Filipe, Raja Ghosh, Marcus Y. Kim, Elena N. Komkova, Alicja M. Mika, Jinsheng Zhou
  • Publication number: 20120142075
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Application
    Filed: February 13, 2012
    Publication date: June 7, 2012
    Applicant: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Publication number: 20120141590
    Abstract: Microspheres are produced by contacting a solution of a macromolecule or small molecule in a solvent with an antisolvent and a counterion, and chilling the solution. The microspheres are useful for preparing pharmaceuticals, nutraceuticals, cosmetic products and the like of defined dimensions.
    Type: Application
    Filed: September 30, 2011
    Publication date: June 7, 2012
    Inventors: Michael Malakhov, Fang Fang
  • Patent number: 8192971
    Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.
    Type: Grant
    Filed: September 16, 2008
    Date of Patent: June 5, 2012
    Assignee: Natrix Separations Inc.
    Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
  • Publication number: 20120135442
    Abstract: The invention relates to a method comprising: providing a molecularly imprinted polymer imprinted with a first peptide or protein; exposing said molecularly imprinted polymer to a supersaturated solution of a second peptide or protein; and forming a nucleus of and/or growing a crystal of said second peptide or protein on said molecularly imprinted polymer.
    Type: Application
    Filed: May 12, 2010
    Publication date: May 31, 2012
    Applicant: UNIVERSITY OF SURREY
    Inventors: Subrayal Medapati Venkt Reddy, Naomi Esther Chayen
  • Publication number: 20120135460
    Abstract: The present invention generally relates to the field of proteinases and more specifically to chymotrypsin. In particular, the present invention relates to recombinant porcine chymotrypsin and its use in food applications.
    Type: Application
    Filed: December 11, 2009
    Publication date: May 31, 2012
    Applicant: NESTEC S.A.
    Inventors: Raymond-David Pridmore, Fabrizio Arigoni, Francoise Maynard, Isabelle Bureau-Franz
  • Patent number: 8084025
    Abstract: A therapeutic agent for the treatment of the symptoms of addiction and the method for preparing the therapeutic agent is disclosed. The therapeutic agent is a stable pharmaceutical preparation containing, but not limited to, digestive/pancreatic enzymes. The therapeutic agent may be manufactured by a variety of encapsulation technologies. Delivery of the therapeutic agent may be made orally, through injection, by adherence of a medicated patch or other method. Further, a method of using of a biomarker, the presence of chymotrypsin in the gastrointestinal tract to determine the presence of symptoms of addiction, and the likelihood of relapsing into addiction is disclosed.
    Type: Grant
    Filed: April 20, 2009
    Date of Patent: December 27, 2011
    Assignee: Curemark LLC
    Inventor: Joan M. Fallon
  • Publication number: 20110280858
    Abstract: The invention generally provides compositions, kits, pharmaceuticals, and methods that promote and enhance wound healing. Such compositions comprise isolated trypsinogen or trypsin polypeptides or isolated polypeptides obtainable from the excretions/secretions (ES) of Lucilia sericata.
    Type: Application
    Filed: April 8, 2011
    Publication date: November 17, 2011
    Applicant: The Secretary of State for Defence
    Inventors: David I. Pritchard, Kevin M. Shakesheff, Adele J. Horobin, Alan Brown
  • Patent number: 7981635
    Abstract: The present invention relates to the production of a variant of recombinant trypsin with increased substrate specificity for arginine versus lysine in non-animal host organisms. Moreover, the present invention relates to a variant of recombinant trypsin and their production. Also provided are use of recombinant porcine pancreatic trypsin variants for cleavage of precursors of insulins, and kits containing the variant of trypsin.
    Type: Grant
    Filed: August 26, 2006
    Date of Patent: July 19, 2011
    Assignees: Sanofi-Aventis Deutschland GmbH, Roche Diagnostics Operations, Inc.
    Inventors: Frank Zocher, Christoph Hoh, Rainer Mueller, Erhard Kopetzki, Frank Geipel, Stephan Glaser
  • Publication number: 20110070607
    Abstract: A chemoenzymatic method for the preparation of a homogeneous glycoprotein or glycopeptide, including (a) providing an acceptor selected from the group consisting of GlcNAc-protein and GlcNAc-peptide; and (b) reacting the acceptor with a donor substrate including an activated oligosaccharide moiety, in the presence of a catalyst comprising endoglycosidase (ENGase), to transfer the oligosaccharide moiety to the acceptor and yield the homogeneous glycoprotein or glycopeptide. The donor substrate includes, in a specific implementation, a synthetic oligosaccharide oxazoline. A related method of glycoprotein or glycopeptide remodeling with a predetermined natural N-glycan or a tailor-made oligosaccharide moiety, and a method of remodeling an antibody including a heterogeneous sugar chain, are also described. The disclosed methodology enables glycoprotein drugs to be modified for prolonged half-life in vivo, reduced immunogenicity, and enhanced in vivo activity, and for targeting and drug delivery.
    Type: Application
    Filed: October 5, 2010
    Publication date: March 24, 2011
    Inventor: Lai-Xi Wang
  • Publication number: 20110055976
    Abstract: A process of producing a protease in a plant or in plant cells, comprising (a) providing a plant comprising a heterologous nucleotide sequence comprising a coding sequence encoding a fusion protein, said fusion protein comprising: an apoplast or plastid signal peptide; a SUMO protein or a derivative of a SUMO protein; and a zymogen of said protease, and (b) expressing said fusion protein.
    Type: Application
    Filed: March 3, 2009
    Publication date: March 3, 2011
    Applicant: ICON GENETICS GMBH
    Inventors: Romy Kandzia, Carola Engler, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Publication number: 20100286380
    Abstract: The present invention relates to methods for pretreating biological samples for extraction of nucleic acid therefrom. The present invention employs a combination of at least one protein denaturant with one or more of the following elements to form a reaction mixture for extraction of nucleic acid: (1) at least one aprotic solvent, (2) stepwise heating, and (3) sample dilution.
    Type: Application
    Filed: May 12, 2010
    Publication date: November 11, 2010
    Applicant: BECTON, DICKINSON AND COMPANY
    Inventors: Matthew P. Collis, Donald Copertino, Karen Eckert, Thomas Fort
  • Publication number: 20100279382
    Abstract: The invention provides compositions and methods for identifying and/or quantifying glycopolypeptides from human serum or plasma. The compositions and methods include a plurality of standard peptides containing glycosylation sites determined for human serum/plasma proteins.
    Type: Application
    Filed: March 12, 2010
    Publication date: November 4, 2010
    Applicant: The Institute for Systems Biology
    Inventors: Rudolf H. Aebersold, Hui Zhang
  • Publication number: 20100254972
    Abstract: The invention relates to a method for selecting, isolating and/or recovering a peptide or polypeptide from a library or a repertoire of peptides and polypeptides (e.g., a display system) that is resistant to degradation by a protease such as a protease found in the GI tract or pulmonary tissue of a human. Generally, the method comprises providing a library or repertoire of peptides or polypeptides, combining the library or repertoire with a protease under conditions suitable for protease activity, and selecting, isolating and/or recovering a peptide or polypeptide that is resistant to degradation by the protease and has a desired biological activity. The selected peptides and polypeptides have utility as therapeutics, eg for treating disease or conditions of GI tract or pulmonary tissue in humans.
    Type: Application
    Filed: June 3, 2008
    Publication date: October 7, 2010
    Applicant: Domantis Limited
    Inventors: Laurent Jespers, Malgorzata Pupecka, Ian Tomlinson, Carolyn Enever
  • Publication number: 20100233146
    Abstract: Disclosed herein are a materials such as a coating, an elastomer, an adhesive, a sealant, a textile finish, a wax, and a filler for such a material, wherein the material includes an enzyme such as an esterase (e.g., a lipolytic enzyme, a sulfuric ester hydrolase, an organophosphorus compound degradation enzyme), an enzyme that degrades a cell wall and/or a cell membrane component (e.g., a lysozyme, a lytic transgrycosylase, a peptidase), and/or a biocidal or biostatic peptide. Also disclosed herein are methods of decontaminating a surface comprising such a material from a chemical substrate of an enzyme such as a lipid or an organophosphorus compound, as well as reducing the growth of a microorganism on or within such a material.
    Type: Application
    Filed: May 29, 2009
    Publication date: September 16, 2010
    Applicant: REACTIVE SURFACES, LTD.
    Inventor: C. Steven McDaniel
  • Publication number: 20100196953
    Abstract: The present invention relates to the production of a variant of recombinant trypsin with increased substrate specificity for arginine versus lysine in non-animal host organisms. Moreover, the present invention relates to a variant of recombinant trypsin and their production. Also provided are use of recombinant porcine pancreatic trypsin variants for cleavage of precursors of insulins, and kits containing the variant of trypsin.
    Type: Application
    Filed: August 26, 2006
    Publication date: August 5, 2010
    Applicants: SANOFI-AVENTIS DEUTSCHLAND GMBH, F. HOFFMAN-LA ROCHE AG
    Inventors: Frank Geipel, Stephan Glaser, Christoph Hoh, Erhard Kopetzki, Rainer Mueller, Frank Zocher
  • Patent number: 7709446
    Abstract: Fusion proteins of protease inhibitors are provided, in particular fusion proteins of alpha 1-antitrypsin (AAT) and a second protease inhibitor, such as secretory leukocyte protease inhibitor (SLPI) or tissue inhibitor of metalloproteases (TIMP). Polynucleotides encoding the fusion proteins, vectors comprising such polynucleotides, and host cells containing such vectors are also provided. Methods of making the fusion proteins of the invention are also provide, as well as methods of using the fusion proteins, for example to inhibit protease activity in a biological sample or in the treatment of an individual suffering from, or at risk for, a disease or disorder involving unwanted protease activity.
    Type: Grant
    Filed: July 20, 2007
    Date of Patent: May 4, 2010
    Assignee: Arrive Pharmaceuticals, Inc.
    Inventors: Philip J. Barr, Helen L. Gibson, Philip A. Pemberton
  • Patent number: 7659380
    Abstract: The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
    Type: Grant
    Filed: April 20, 2007
    Date of Patent: February 9, 2010
    Assignee: Baxter Healthcare S.A.
    Inventors: Artur Mitterer, Christa Tauer, Manfred Reiter, Wolfgang Mundt
  • Publication number: 20100008898
    Abstract: The use of larval enzymes, particularly a chymotrypsin, is described herein. The enzymes are usable in the treatment of wounds for debridement and for cell regeneration.
    Type: Application
    Filed: May 31, 2007
    Publication date: January 14, 2010
    Inventors: David Idris Pritchard, Adele J. Horobin, Alan Brown
  • Publication number: 20090324608
    Abstract: The invention provides isolated nucleic acids molecules, designated 53070, 15985, 26583, 21953, m32404, 14089, and 23436 nucleic acid molecules, which encode novel human protein kinase family members, serine/threonine protein kinase family members, serine/threonine phosphatase family members, prolyl oligopeptidase family members, trypsin family members, trypsin serine protease family members, and ubiquitin protease family members. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing 53070, 15985, 26583, 21953, m32404, 14089, or 23436 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a 53070, 15985, 26583, 21953, m32404, 14089, or 23436 gene has been introduced or disrupted.
    Type: Application
    Filed: September 5, 2007
    Publication date: December 31, 2009
    Inventors: Rachel E. Meyers, Peter J. Olandt, Rosana Kapeller-Libermann, Rory A. J. Curtis, Mark Williamson, Nadine Weich
  • Publication number: 20090269830
    Abstract: The present invention relates to a culture system for and a method for propagation of human blastocyst-derived stem cells (hBS cells) upon enzymatic dissociation into a single cell suspension. The culture system for propagation of human blastocyst-derived stem (hBS) cells comprises i) human feeder cells at a density of at least 50,000 cells/cm2, ii) one or more dissociation agents for dissociation of hBS cell colonies into a single cell suspension, and iii) a supportive culture medium, which culture system makes it possible to propagate hBS cells by dissociation of hBS cell colonies into a single cell suspension at each consecutive passage for an extended time period, while maintaining the significant characteristics of hBS cells.
    Type: Application
    Filed: March 16, 2007
    Publication date: October 29, 2009
    Applicant: Cellartis AB
    Inventors: Henrik Semb, Raimund Strehl, Catharina Ellerstrom
  • Publication number: 20090252720
    Abstract: The invention is related to FIX analogues which have an increased circulation time in the blood stream before activation compared to that that of native FIX (and a week after injection to a patient retains at least about 5% of the FIX activity compared to the initial activity peak value reached after injection). The claimed FIX analogues comprise an inserted cysteine residue which has been further modified by conjugation with a chemical group increasing the molecular weight of the FIX analogue.
    Type: Application
    Filed: May 24, 2007
    Publication date: October 8, 2009
    Applicant: Novo Nordisk Health Care AG
    Inventors: Henrik Ostergaard, Ole Hvilsted Olsen, Henning Ralf Stennicke, Thomas Dock Steenstrup