Chemical Stimulation Of Growth Or Activity By Addition Of Chemical Compound Which Is Not An Essential Growth Factor; Stimulation Of Growth By Removal Of A Chemical Compound Patents (Class 435/244)
  • Patent number: 10202571
    Abstract: This invention provides devices and methods that enable co-incubation of microorganisms. Also provided are methods of making such devices for co-incubation of microorganisms, and various applications of such devices.
    Type: Grant
    Filed: June 22, 2015
    Date of Patent: February 12, 2019
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: James Q. Boedicker, Rustem F. Ismagilov, Hyun Jung Kim
  • Patent number: 10076120
    Abstract: This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: September 18, 2018
    Assignee: Indigo Agriculture, Inc.
    Inventors: Geoffrey von Maltzahn, Richard Bailey Flavell, Gerardo V. Toledo, Slavica Djonovic, Luis Miguel Marquez, David Morris Johnston, Yves Alain Millet, Jeffrey Lyford, Alexander Naydich, Craig Sadowski
  • Patent number: 10058101
    Abstract: This application relates to methods and materials for providing a benefit to a seed or seedling of an agricultural plant (e.g., an agricultural grass plant), or the agricultural plant derived from the seed or seedling. For example, this application provides purified bacterial populations that include novel seed-origin bacterial endophytes, and synthetic combinations of seeds and/or seedlings (e.g., cereal seeds and/or seedlings) with heterologous seed-derived bacterial endophytes.
    Type: Grant
    Filed: February 17, 2017
    Date of Patent: August 28, 2018
    Assignee: Indigo Agriculture, Inc.
    Inventors: Geoffrey von Maltzahn, Richard Bailey Flavell, Gerardo V. Toledo, Slavica Djonovic, Luis Miguel Marquez, David Morris Johnston, Yves Alain Millet, Jeffrey Lyford, Alexander Naydich, Craig Sadowski
  • Patent number: 9359623
    Abstract: Provided herein are methods and compositions for the production of renewable fatty acids from photosynthetic prokaryotic microorganisms, such as a blue green algae, any specie of the phylum Cyanophyta, a chloroplast of a green algae and/or a Cyanobacterium. Engineered or natural strains of these organisms or organelles can be used to produce both saturated and unsaturated fatty acids with variable chain length specificity from 8-18 carbons. The fatty acids can then be secreted into the culture medium, allowing for rapid, continuous and efficient separation of fatty acid product without harvesting of cell mass.
    Type: Grant
    Filed: July 22, 2010
    Date of Patent: June 7, 2016
    Assignee: The Regents of the University of California
    Inventors: Steve A. Kay, Ewa Lis, Susan Golden, Michael Melnick, Dawn M. Adin, James W. Golden
  • Patent number: 9347083
    Abstract: A medium for the culture and detection of target microorganisms, having at least one natural or synthetic specific substrate configured to detect at least one enzyme activity or metabolic activity of the target microorganisms and at least one compound that inhibits or delays the germination of spores of microorganisms, other than the target microorganisms, that are capable of interfering with the culture and detection of the target microorganisms.
    Type: Grant
    Filed: June 23, 2009
    Date of Patent: May 24, 2016
    Assignee: BIOMERIEUX
    Inventor: Aurelien Costa
  • Publication number: 20150147792
    Abstract: A method of making a C4 diacid from a sugar source by fermentation with a microorganism is described that includes supplementing the fermentation medium with relatively low amounts of betaine. The use of betaine can substantially reduce or even completely eliminate the need for complex nutrient source such as yeast extract, and eliminate a need for vitamins such as biotin, while maintaining a high level of production of the C4 diacid from a simplified sugar based media. The use of betaine improves yield from sugar, final titer, and rate of production per hour. The method is particularly suitable for microorganisms that otherwise display a dependence on yeast extract for high productivity, more particularly for any bacteria, and most particularly for the production of succinic acid from the bacterium Actinobacillus succinogenes. An improved strain of A succinogenes is also described, having a mutation in a betaine transporter that improves C4 diacid production in the presence of betaine.
    Type: Application
    Filed: June 27, 2013
    Publication date: May 28, 2015
    Inventor: Jill Morriss
  • Patent number: 9034632
    Abstract: This disclosure provides compositions and methods for culturing microorganisms. The disclosure includes mixtures of different microorganisms, especially mixtures of cyanobacteria with fermentative microorganisms. For example, methods and compositions related to co-cultures of yeast and cyanobacteria are provided. Also provided are feedstocks derived from cyanobacteria as well as methods of making such feedstocks and methods of culturing microorganisms in such feedstocks.
    Type: Grant
    Filed: December 19, 2012
    Date of Patent: May 19, 2015
    Assignee: HelioBioSys, Inc.
    Inventors: Rocco Mancinelli, David Smernoff
  • Patent number: 9029118
    Abstract: Selective enrichment media and methods for selectively growing and detecting Salmonella spp. The media comprise a carbon and nitrogen source, an inorganic salt, a fermentable sugar, one or more selective agents, and an efflux pump inhibitor. Various selective agents include sulfa drugs, surfactants, aminocoumarins, cycloheximide, supravital stains, ascorbic acid, bromobenzoic acid, myricetin, rifamycins, polyketides, and oxazolidinones. Various efflux pump inhibitors include arylpiperazines, such as 1-(1-naphthylmethyl)piperazine, and quinoline derivatives, such as 4-chloroquinoline. The selective agents and efflux pump inhibitors are provided in the media in combinations and amounts that inhibit growth of non-Salmonella microorganisms without substantially affecting growth and metabolism of Salmonella species. Methods of selectively growing and detecting Salmonella species are provided.
    Type: Grant
    Filed: February 27, 2013
    Date of Patent: May 12, 2015
    Assignee: Paradigm Diagnostics, Inc.
    Inventor: Alan D Olstein
  • Publication number: 20150104868
    Abstract: The present invention relates to methods of using meso-1,2,3,4-tetrahydroxybutane for the maintenance and/or improvement of biological cell function and activity, and for the prevention of improper cell functioning or cell death, in vitro, ex vivo, and in vivo over time and/or during exposure to stress. Meso-1,2,3,4-tetrahydroxybutane can be used to promote cell survival and as a cell protection agent, to increase cell viability, and to improve conversion of progenitor or stem cells to mature cells, whether in vitro, in vivo, ex-vivo, or transplanted.
    Type: Application
    Filed: April 19, 2013
    Publication date: April 16, 2015
    Applicant: CARGILL, INC.
    Inventors: Alvin Berger, Aalt Bast, Petrus Wilhelmus Hubertus De Cock, Gerardus Johannes Martinus Den Hartog, Jean-Claude Marie-Pierre Ghislain De Troostembergh
  • Publication number: 20150099667
    Abstract: The present invention relates to a method for the activation of silent genes in microorganisms by co-cultivation of an inducer and a recipient microorganism. The inducer is selected from a chemical inducer, a microorganism inducer which is selected from a killed microorganism cell and/or inactivated culture medium in which said microorganism cell had been cultured and/or medium inducer. The present invention furthermore relates to a method for screening for an inducer and to a method of screening for a recipient microorganism by co-cultivation of an inducer and a recipient organism. The methods are useful for the detection of medicaments, such as antibiotics. The present invention further relates to media for culturing microorganisms comprising an inducer.
    Type: Application
    Filed: May 13, 2013
    Publication date: April 9, 2015
    Applicant: Sanofi
    Inventors: Joachim Wink, Stefan Bartoschek, Andreas Batzer, Stéphane Renard
  • Patent number: 8999664
    Abstract: The present invention provides a salmon flesh color improving method and a salmon flesh color improving feed which are usable for producing a salmon having a mild reddish orange flesh color close to the flesh color of wild fish, and a salmon and fish flesh thereof produced by such a flesh color improving method. The present invention also provides a method for producing a salmon having a mild reddish orange flesh color, comprising cultivating the salmon with a feed comprising a carotenoid colorant mixed therein, the carotenoid colorant comprising at least astaxanthin, phoenicoxanthin, canthaxanthin and adonixanthin.
    Type: Grant
    Filed: September 14, 2007
    Date of Patent: April 7, 2015
    Assignee: JX Nippon Oil & Energy Corporation
    Inventors: Kazuaki Hirasawa, Akira Tsubokura, Hisashi Yoneda
  • Patent number: 8927246
    Abstract: A method of increasing the production of extracellular polymeric substances (EPS) in an Acidithiobacillus ferrooxidans culture is disclosed. The method includes inhibiting an enzyme, such as citrate synthase, aconitase, or isocitrate dehydrogenase, in the tricarboxylic acid (TCA) cycle leading to alpha-ketoglutarate.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: January 6, 2015
    Assignees: Universidad de Chile, BioSigma S.A.
    Inventors: Alejandro Eduardo Maass Sepúlved, Pablo Andrés Moreno Cortéz, Marko Antonio Budinich Abarca, Pilar Angélica Parada Valdecantos, Leandro Mauricio Padilla Iglesias, Marlen Nayibe Barreto Roa
  • Publication number: 20140363819
    Abstract: Provided herein are compositions and methods for improving amplification or detection of a target nucleic acid in a sample containing PCR inhibitors, such as polyphenols. One aspect provides an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof. Another aspect provides a method of amplifying a target nucleic acid with an enhancer composition including casein or polyvinylpyrrolidone, or a modified polymer thereof. Another aspect provides a method for culturing microorganisms in media containing PVP or casein.
    Type: Application
    Filed: December 22, 2011
    Publication date: December 11, 2014
    Inventors: Katherine Rowlyk, Zhian Zhang, Milko Kermekchiev
  • Publication number: 20140349375
    Abstract: Disclosed herein are methods of use of methylsulfonylmethane (MSM) to modulate microbial activity, such as to enhance or inhibit the activity of microorganisms. In one example, MSM (such as about 0.5% to 5% MSM) is used to enhance fermentation efficiency, such as to enhance fermentation efficiency associated with the production of beer, cider, wine, a biofuel, dairy product or any combination thereof. Also disclosed are in vitro methods for enhancing the growth of one or more probiotic microorganisms and methods of enhancing growth of a microorganism in a diagnostic test sample. Methods of inhibiting microbial activity are also disclosed. In one particular example, a method of inhibiting microbial activity includes selecting a medium that is susceptible to H1N1 influenza contamination; and contacting the medium with MSM at a concentration of about 10% to about 16% of weight by volume, thereby inhibiting H1N1 influenza microbial activity.
    Type: Application
    Filed: August 12, 2014
    Publication date: November 27, 2014
    Inventors: Rodney L. Benjamin, Jeffrey Varelman, Anthony L. Keller
  • Patent number: 8859228
    Abstract: The invention relates to a novel method for the production of ?-carotene from submerged cultures of mucoral fungi such as Blakeslea, Choanephora or Phycomyces by adding lectin to the culture medium and performing pH control once fermentation has started. The method involves ?-carotene recovery stage that makes it possible to simplify the process, optimize yields and increase purification of the product.
    Type: Grant
    Filed: July 18, 2001
    Date of Patent: October 14, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Javier Costa Perez, Antonio Estrella Castro, Ana Teresa Marcos Rodriguez, J. Emiliano Gonzalez De Prado, Enrique R. Peiro Cezon, Alfonso Collados De La Vieja, Manuel Esteban Morales
  • Publication number: 20140295530
    Abstract: A method for increasing the content of ubiquinone (CoQ10) and ubiquinol (CoQH2) in phototrophic microorganisms that were cultivated in a culture medium in a bioreactor under light irradiation, wherein the phototrophic microorganisms are selected from the group consisting of blue algae, green algae and yellow-green algae, comprising a step of inducing oxidative stress. By virtue of the fact that oxidative stress was induced by incubating the phototrophic microorganisms together with Fe3+ in the culture medium, a higher content of CoQ10 and CoQH2 is obtained. Moreover, the microorganisms thus obtained have a higher content of trivalent iron, which is particularly relevant for the human diet. From the phototrophic microorganisms it is possible to produce an oily extract and also a dried algae product.
    Type: Application
    Filed: December 21, 2011
    Publication date: October 2, 2014
    Applicant: SANBO INTERNATIONAL ESTABLISHMENT
    Inventor: Oliver Durr
  • Patent number: 8835156
    Abstract: The present disclosure provides a method for pre-treating non-wood lignocellulosic material containing less than 5 % (w/w) starch or sugar in a process for production of ethanol from lignocellulose, comprising the steps of: adding organic acid or organic acid-producing bacteria to the lignocellulosic material; storing the lignocellulosic material in the presence of the organic acid for a period of at least two weeks in an atmosphere of less than 5% oxygen to obtain organic acid-impregnated material; and heating the organic acid-impregnated material at a temperature of at least 160° C. to obtain pre-treated lignocellulosic material, wherein no, or substantially no, inorganic acid or base, including SO2, is added in the method.
    Type: Grant
    Filed: June 17, 2010
    Date of Patent: September 16, 2014
    Assignee: Sekab E-Technology AB
    Inventors: Lovisa Bjornsson, Sven-Erik Svensson, Sanam Monavari, Emma Kreuger, Guido Zacchi
  • Patent number: 8835152
    Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.
    Type: Grant
    Filed: December 4, 2012
    Date of Patent: September 16, 2014
    Assignee: Advanced Biocatalytics Corporation
    Inventor: Carl W. Podella
  • Patent number: 8828696
    Abstract: A process for the use of low concentration levels of Penicillin G Procaine to eliminate or control the growth of unwanted or undesirable bacteria (contaminating bacteria) in the fermentation production of alcohol without inhibition of the growth or replication of the yeast.
    Type: Grant
    Filed: December 5, 2008
    Date of Patent: September 9, 2014
    Assignee: Lallemand Specialites, Inc
    Inventors: Kevin E. Dailey, Sanjoy Ganguly, Kevin L. Kauers, Limei Tang, Daniel W. Thon
  • Patent number: 8815565
    Abstract: Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.
    Type: Grant
    Filed: November 24, 2010
    Date of Patent: August 26, 2014
    Assignee: Cellsafe Co., Ltd.
    Inventor: Jae-Jin Han
  • Patent number: 8809034
    Abstract: Provided is a method for culturing cells, which prevents the possibility of mycoplasma contamination by culturing the cells using biomass extract obtained by culturing a strain with amphidinol productivity, preferably a strain such as Amphidinium klebsii, Amphididinium carterae, and the like belonging to Dinoflagellates, or using a fraction obtained from the extract; and a method for removing mycoplasma contamination of the cells by culturing the cells contaminated by mycoplasma using the amphidinol derivatives. In addition, provided is a method for removing mycoplasma contamination of the cells, including a combination of single cell-isolating enzyme solution treatment and cell aggregate removal.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: August 19, 2014
    Assignee: Cellsafe Co., Ltd.
    Inventor: Jae-Jin Han
  • Publication number: 20140199372
    Abstract: Disclosed are methods of cultivating or isolating a microorganism using one or more quinones as growth factors. Also disclosed are methods of treating a mammalian species with deficiency in symbionts using such compounds.
    Type: Application
    Filed: May 24, 2012
    Publication date: July 17, 2014
    Applicant: NORTHEASTERN UNIVERSITY
    Inventors: Kim Lewis, Pallavi Murugkar, Anthony D'onofrio, Eric Stewart, Eric Dimise, Jon Clardy, Kathrin Witt, Bijaya Sharma
  • Patent number: 8778643
    Abstract: The present disclosure relates to methods of increasing lipid levels in an algal or yeast cell population, methods of producing saturated or monounsaturated triacylglycerols in an algal or yeast cell population, and methods of decreasing polyunsaturated triacylglycerol production in an algal or yeast cell population by contacting the cell population with a chemical compound that is capable of increasing lipid levels or altering the lipid composition in the cell population.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: July 15, 2014
    Assignee: The Regents of the University of California
    Inventors: Annaliese Kirsten Franz, Megan Danielewicz, Diana Meiying Wong, Lisa Adele Anderson
  • Patent number: 8765399
    Abstract: Disclosed are culture media, protocols and kits for diagnosis of toxigenic Clostridium difficile, where the culture medium comprises Cooked Meat Medium with glucose; yeast extract; taurocholate; cycloserine; and cefoxitin.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: July 1, 2014
    Assignee: Montefiore Medical Center
    Inventor: Paul Riska
  • Patent number: 8759068
    Abstract: The system for fermentation using algae of the present invention includes a first reactor and a second reactor being in fluid communication with each other. A first valve placed between the first reactor and the second reactor controls the fluid connection between the reactors. A gas inlet, in fluid connection to the first reactor, is located at an end opposite the second reactor. A devolatization unit or cell lysis chamber is connected to the second reactor by a second valve. A biomass stream having gas, liquid and biosolids contents passes through the first reactor with gas. The biomass stream mixes and dissolves the gas in the reactors. The cellular structure of the biomass stream ruptures in the devolatization unit, allowing the processed materials, such as oil, gas, and biosolids, to be harvested for use.
    Type: Grant
    Filed: May 5, 2008
    Date of Patent: June 24, 2014
    Assignee: Missing Link Technologies, L.L.C.
    Inventors: Samuel L. Shepherd, Jerry McCall
  • Patent number: 8753863
    Abstract: Disclosed is a method for removing carbon dioxide from the atmosphere. The method comprises the step of delivering urea from a floating vessel to a region of a photic zone of the ocean, whereby the number of phytoplankton is caused to increase in the region upon addition of the urea. The method can be used for producing carbon cells.
    Type: Grant
    Filed: June 22, 2007
    Date of Patent: June 17, 2014
    Assignee: Ocean Nourishment Corporation Pty Limited
    Inventors: Ian Stanley Ferguson Jones, William Rodgers, Robert John Wheen, Bruce Joseph Judd
  • Patent number: 8703470
    Abstract: Embodiments of the invention relate to the microbial production of polyhydroxyalkanoic acids, or polyhydroxyalkanoates (PHA), from substrates which cannot be used as a source of carbon and/or energy for microbial growth or PHA synthesis and which have microbial and environmental toxicity. According to one embodiment of the invention, a process for the production of PHA is provided wherein an enzyme such as methane monooxygenase is used to convert volatile organic compounds into PHA through the use of microorganisms that are unable to use volatile organic compounds as a source of carbon for growth or PHA production.
    Type: Grant
    Filed: September 10, 2012
    Date of Patent: April 22, 2014
    Assignee: Newlight Technologies, LLC
    Inventors: Markus Herrema, Kenton Kimmel
  • Patent number: 8673617
    Abstract: The invention relates to a culture medium for Haemophilus influenzae type b, characterized in that the source of protein nitrogen is of non-animal origin and comprises at least one plant peptone and in that the heme source consists of protoporphyrin IX. This medium serves in particular for the production of polyribosyl phosphate (PRP) and for the manufacture of a vaccine against Haemophilus influenzae type b meningitis.
    Type: Grant
    Filed: July 10, 2008
    Date of Patent: March 18, 2014
    Assignee: Sanofi Pasteur SA
    Inventors: Ghislaine Maitre-Wilmotte, Denis Speck, Bachra Rokbi
  • Patent number: 8669090
    Abstract: Methods for production of highly unsaturated fatty acids by marine microorganisms, including the heterotrophic marine dinoflagellate Crypthecodinium, using low levels of chloride ion are disclosed. Specifically, methods of increasing production of highly unsaturated fatty acids by marine microorganisms while growing in low chloride media by manipulating sodium ion and potassium ion levels. The invention also relates to methods of production of highly unsaturated fatty acids by marine organisms at low pH levels, and includes methods for generation of low pH tolerant strains.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: March 11, 2014
    Assignee: DSM IP Assets B.V.
    Inventors: Paul W. Behrens, John M. Thompson, Kirk Apt, Joseph W. Pfeifer, III, James P. Wynn, James Casey Lippmeier, Jaouad Fichtali, Jon Hansen
  • Patent number: 8658411
    Abstract: The problem addressed by the present invention is to provide a method of treating an organic substance-containing waste liquid, and a treatment apparatus therefor, and an additive for treating an organic substance-containing waste liquid, and a bacterium for degrading an organic substance component or the like in the waste liquid, capable of accomplishing at least one of treatment of the organic substance-containing waste liquid with a high efficiency and a high treatment rate, without substantially generating a foul odor, and obtainment of water quality that is suitable for releasing to sewage, a public water area, or the like. This problem is solved by providing a method of treating an organic substance-containing waste liquid characterized by contacting an activated sludge containing a specified bacterium with an organic substance-containing waste liquid.
    Type: Grant
    Filed: April 21, 2006
    Date of Patent: February 25, 2014
    Assignee: Ibiden Co., Ltd.
    Inventors: Ryozo Irie, Michio Tabata, Yoshihisa Hibi
  • Patent number: 8642316
    Abstract: A process for the culture of Leptospires in a protein-free culture medium and without any fatty acid source in the culture medium, wherein a fatty acid source is fed continuously or intermittently.
    Type: Grant
    Filed: April 14, 2006
    Date of Patent: February 4, 2014
    Assignee: Merial Limited
    Inventor: Nicolas Pierre Yves Carboulec
  • Publication number: 20130344558
    Abstract: Wheat bran culture mix is used to inoculate organic waste and produce an inoculated waste material, where the wheat bran culture mix includes soil derived microbes. The inoculated waste material is shredded to produce shredded inoculated waste material which is fermented the shredded inoculated waste material for at least 7 days. Contents from the fermenter are then transferred into a dewatering device to produce dewatered contents which are then separated into soluble and suspended products.
    Type: Application
    Filed: March 9, 2012
    Publication date: December 26, 2013
    Applicant: BOKASHICYCLE NVC LLC
    Inventor: Lawrence R. Green
  • Publication number: 20130341266
    Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.
    Type: Application
    Filed: December 4, 2012
    Publication date: December 26, 2013
    Inventor: Carl W. PODELLA
  • Publication number: 20130337522
    Abstract: Contamination was controlled in fermentations using Zymomonas mobilis as the biocatalyst, without negative impact on fermentation production, by the addition of virginiamycin. The effective concentration of virginiamycin was found to be dependent upon the type of fermentation medium used.
    Type: Application
    Filed: June 15, 2012
    Publication date: December 19, 2013
    Applicant: E I DU PONT DE NEMOURS AND COMPANY
    Inventors: Maria C. Leana, Brian G. Lefebvre
  • Publication number: 20130288294
    Abstract: A method and point of use kit to determine under field conditions the presence and/or amount of a selected Pseudomonas species capable of bioremediating organic environmental pollutants, such as petroleum hydrocarbons.
    Type: Application
    Filed: April 24, 2013
    Publication date: October 31, 2013
    Inventors: Michael T. Saul, Sergey Gazenko
  • Patent number: 8569014
    Abstract: A carotenoid producing bacterium belonging to the genus Paracoccus that selectively produces canthaxanthin so that the amount thereof is not less than 90 percent by weight of the total amount of produced carotenoids including ?-carotene, ?-cryptoxanthin, echinenone, canthaxanthin, 3-hydroxyechinenone, 3?-hydroxyechinenone, zeaxanthin, phoenicoxanthin, adonixanthin, and astaxanthin. A method for producing canthaxanthin by culturing the above bacterium, and then collecting carotenoids from bacterial cells or a culture solution after the culturing.
    Type: Grant
    Filed: August 10, 2011
    Date of Patent: October 29, 2013
    Assignee: Tosoh Corporation
    Inventors: Toru Tanaka, Teruhiko Ide
  • Publication number: 20130273620
    Abstract: The present disclosure relates to methods of increasing lipid levels in an algal or yeast cell population, methods of producing saturated or monounsaturated triacylglycerols in an algal or yeast cell population, and methods of decreasing polyunsaturated triacylglycerol production in an algal or yeast cell population by contacting the cell population with a chemical compound that is capable of increasing lipid levels or altering the lipid composition in the cell population.
    Type: Application
    Filed: March 13, 2013
    Publication date: October 17, 2013
    Inventors: Annaliese Kirsten FRANZ, Megan DANIELEWICZ, Diana Meiying WONG, Lisa Adele ANDERSON
  • Publication number: 20130266955
    Abstract: A latent effervescent body comprising a selective agent is disclosed. A method of using the latent effervescent body in a method to selectively enrich a target microorganism is also disclosed. The method comprises providing a sample, a culture medium, and the latent effervescent body. The method further comprises contacting the sample, the culture medium, and the latent effervescent body under conditions to facilitate growth of the target microorganism. The method further comprises releasing the selective agent from the latent effervescent body. Optionally, the method includes detecting a microorganism.
    Type: Application
    Filed: December 22, 2011
    Publication date: October 10, 2013
    Inventors: Wensheng Xia, Patrick A. Mach, Joseph M. Beaurline, Jason W. Bjork, Jie J. Liu
  • Publication number: 20130183685
    Abstract: A method for recombinantly expressing a macromolecule in a host cell is disclosed which involves culturing a host cell which contains two nucleic acid sequences, i.e., a first nucleic acid sequence encoding a membrane-permeabilizing agent and a second nucleic acid sequence encoding a desired macromolecule under the operative control of an inducible promoter, to a selected cell density that permits accumulation of the agent. Thereafter the host cell is exposed to an environmental condition that induces the agent to disrupt the integrity of the cell membrane without complete lysis of the cell membrane. The host cell thereby allows transport through the membrane of small molecular weight compounds. These resulting host cells are cultured in the presence of a nutrient cocktail that contains components that can transport through the disrupted cell membrane, e.g., an inducing agent that induces the tightly regulated promoter and metabolic requirements that permit expression of the macromolecule.
    Type: Application
    Filed: September 23, 2011
    Publication date: July 18, 2013
    Applicant: Da Yu Enterprises, L.L.C.
    Inventor: Donald O'Keefe
  • Publication number: 20130183732
    Abstract: This disclosure provides compositions and methods for culturing microorganisms. The disclosure includes mixtures of different microorganisms, especially mixtures of cyanobacteria with fermentative microorganisms. For example, methods and compositions related to co-cultures of yeast and cyanobacteria are provided. Also provided are feedstocks derived from cyanobacteria as well as methods of making such feedstocks and methods of culturing microorganisms in such feedstocks.
    Type: Application
    Filed: December 19, 2012
    Publication date: July 18, 2013
    Applicant: HELIOBIOSYS, INC.
    Inventor: HELIOBIOSYS, INC.
  • Publication number: 20130164822
    Abstract: The present invention is directed to a method and means for the neutralization, binding, and/or inactivation of antimicrobials in a test sample. The invention is also directed to a method of detecting the presence of one or more microorganisms in a test sample by culturing the test sample in a culture media comprising one or more primary amine-containing compounds.
    Type: Application
    Filed: February 21, 2013
    Publication date: June 27, 2013
    Applicant: bioMerieux, Inc.
    Inventor: Douglas Lovern
  • Patent number: 8470311
    Abstract: The present invention provides a therapeutic agent for anaerobic diseases such as solid tumor, comprising in combination a pharmaceutical composition for the treatment of an anaerobic disease containing the transformed anaerobic microorganism as an active component and a pharmaceutical composition containing as an active component an anaerobic microorganism colonization and growth enhancer for enhancing the specific colonization and proliferation of the anaerobic microorganism at an anaerobic disease site. Furthermore, the present invention provides to an anaerobic microorganism colonization and growth enhancer for enhancing colonization and growth of the transformed anaerobic microorganism at a disease site that is in an anaerobic environment.
    Type: Grant
    Filed: April 17, 2009
    Date of Patent: June 25, 2013
    Assignee: Anaeropharma Science, Inc.
    Inventors: Takayuki Sasaki, Hitomi Shimizu, Yuko Shimatani-Shibata, Hiromi Yonekura
  • Publication number: 20130149415
    Abstract: The invention relates to a method for selectively enhancing the growth of bacteria from the Lactobacillus casei group in a mixed culture, by adding manganese to the culture medium. Said method is suitable in particular in the case of mixed cultures associating Lactobacillus casei with yogurt bacteria.
    Type: Application
    Filed: July 15, 2010
    Publication date: June 13, 2013
    Inventors: Laurent Marchal, Cyril Colin
  • Patent number: 8449877
    Abstract: The present invention provides a method for purifying bacterial minicells that involves subjecting a sample containing minicells to density gradient centrifugation in a biologically compatible medium. The method optionally includes a preliminary differential centrifugation step and one or more filtration steps. The invention also provides a method for purifying bacterial minicells in which a sample containing minicells is subjected to a condition that induces parent bacterial cells to adopt a filamentous form, followed by filtration of the sample to separate minicells from parent bacterial cells. The inventive methods optionally include one or more steps to remove endotoxin from purified minicell preparations, and/or treatment of purified minicell preparations with an antibiotic. Additionally, the invention provides purified minicell preparations, prepared according to the foregoing methods, and containing fewer than about 1 contaminating parent bacterial cell per 107, 108, 109, 1010, or 1011 minicells.
    Type: Grant
    Filed: April 4, 2011
    Date of Patent: May 28, 2013
    Assignee: Engeneic Molecular Delivery Pty. Ltd.
    Inventors: Himanshu Brahmbhatt, Jennifer MacDiarmid
  • Patent number: 8445277
    Abstract: Certain embodiments disclosed herein include, but are not limited to, at least one of compositions, methods, devices, systems, kits, or products regarding rejuvenation or preservation of germ cells or gametes. Certain embodiments disclosed herein include, but are not limited to, methods of modifying germ cells or gametes, or methods of administering modified germ cells or gametes to at least one biological tissue.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: May 21, 2013
    Assignee: The Invention Science Fund I, LLC
    Inventors: Roderick A. Hyde, Edward K. Y. Jung, Lowell L. Wood, Jr.
  • Publication number: 20130122565
    Abstract: The present invention is directed to methods for inducing desired activity in enzymes or microorganisms capable of producing the enzymes. The invention is further directed to methods of stabilizing activity in microorganisms. In specific embodiments, the invention provides methods for inducing and stabilizing nitrile hydratase activity, amidase activity, and asparaginase I activity. The invention further provides compositions comprising enzymes or microorganisms having induced and/or stabilized activity.
    Type: Application
    Filed: November 2, 2012
    Publication date: May 16, 2013
    Applicant: GEORGIA STATE UNIVERSITY RESEARCH FOUNDATION, INC.
    Inventor: Georgia State University Research Foundation, Inc
  • Patent number: 8426189
    Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.
    Type: Grant
    Filed: April 27, 2010
    Date of Patent: April 23, 2013
    Assignee: Fermalogic, Inc.
    Inventor: J. Mark Weber
  • Patent number: 8338143
    Abstract: This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: December 25, 2012
    Assignee: Phyton Holdings, LLC
    Inventors: Venkataraman Bringi, Prakash Kadkade, Christopher Prince, Braden Roach
  • Publication number: 20120322107
    Abstract: The invention encompasses the use of one or more compounds selected from a list comprising i) reduced forms of vitamin K and/or ii) reduced forms of a vitamin K analog and/or iii) reduced forms of a vitamin K precursor for the expression of one or more functional vitamin K-dependent proteins in cell culture as well as processes for the fermentation of eucaryotic cells expressing one or more vitamin K-dependent proteins wherein one or more compounds selected from a list comprising i) reduced forms of vitamin K and/or ii) reduced forms of a vitamin K analog and/or iii) reduced forms of a vitamin K precursor are added to the cell culture medium before and/or during the fermentation process.
    Type: Application
    Filed: July 9, 2010
    Publication date: December 20, 2012
    Applicant: CSL LIMITED
    Inventors: Julian Keane, Anthony Stowers, Peter Soupourmas, Fraser Goodwin
  • Patent number: 8323949
    Abstract: Compositions of peptides and surface-active agents are described, as are methods of making and using such compositions. The compositions are capable of affecting metabolic rates in biological systems, and to accelerate nutrient uptake without a concomitant increase in biofilm production.
    Type: Grant
    Filed: December 15, 2008
    Date of Patent: December 4, 2012
    Assignee: Advanced BioCatalytics Corporation
    Inventor: Carl W. Podella