Chemical Stimulation Of Growth Or Activity By Addition Of Chemical Compound Which Is Not An Essential Growth Factor; Stimulation Of Growth By Removal Of A Chemical Compound Patents (Class 435/244)
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Patent number: 6893857Abstract: The invention relates to a method for controlling microorganisms in a sugar-containing aqueous process medium, especially in the sugar industry, using hop bitter acid as active substance. The hop bitter acid is dissolved in an aqueous alkaline medium and added to the process medium. The pH value of the added solution is higher than the pH value of the process medium and the hop bitter acid passes from the dissociated to the indissociated form in the process medium.Type: GrantFiled: February 22, 2000Date of Patent: May 17, 2005Assignees: Beta Tec Hopfenprodukte GmbH, Zuckerforschung Tulln GmbHInventors: John-Paul Maye, David Beddie, Gunter Pollach
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Patent number: 6872562Abstract: The present invention is directed to herbicide resistant N2 fixing bacteria. The bacteria are useful for effecting N2 fixation, nodulation, growth and yield of herbicide resistant or tolerant leguminous plants treated with herbicide. The bacteria are particularly useful for providing competitive advantage to superior N2 fixing rhizobial strains over non-resistant indigenous rhizobia for nodulation of herbicide resistant or tolerant leguminous plants.Type: GrantFiled: January 16, 2002Date of Patent: March 29, 2005Assignee: The Board of Trustees of the University of Arkansas, N.A.Inventors: Charles A. King, Larry C. Purcell
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Patent number: 6867023Abstract: Polyhydroxyalkanoates comprising a monomer unit having the following chemical formula (2): (where the reference characters R represents an arbitrarily selected substituent and x represents an integer of 0 to 8) is produced by culturing a microorganism in a culture medium containing a substituted fatty acid ester having the following chemical formula (1): (where the reference characters R and R? separately denote an optional substituent and x represents an integer of 0 to 8). The microorganism is capable of taking the substituted fatty acid ester into the cells and synthesizing the desired polyhydroxyalkanoate in the culture medium.Type: GrantFiled: October 30, 2001Date of Patent: March 15, 2005Assignee: Canon Kabushiki KaishaInventors: Tsutomu Honma, Tetsuya Yano, Takeshi Imamura, Takashi Kenmoku
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Patent number: 6855536Abstract: The subject invention relates to compounds and compositions which induce transcripts of the nolA gene in nitrogen-fixing bacteria, such as Bradyrhizobium japonicum. Novel bacterial strains which are insensitive too NolA, soil inoculants comprising NolA insensitive bacteria and/or nolA inducers, and methods of increasing nitrogen fixation in legumes are also disclosed.Type: GrantFiled: July 20, 2001Date of Patent: February 15, 2005Assignee: University of Tennessee Research Corp., Inc.Inventors: John T. Loh, Gary Stacey
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Patent number: 6846667Abstract: The present invention is based on the identification of a series of virulence genes in E. coli K1, the products of which may be implicated in the pathogenicity of the organisms. The identification of the genes allows them, or their expressed products, to be used in a number of ways to treat infection.Type: GrantFiled: November 9, 1999Date of Patent: January 25, 2005Assignee: Microscience, Ltd.Inventors: Helen Rachel Crooke, Enda Elizabeth Clarke, Paul Howard Everest, Gordon Dougan, David William Holden, Jacqueline Elizabeth Shea, Robert Graham Feldman
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Patent number: 6843961Abstract: Methods and apparatuses are provided for inactivation of pathogens in fluids containing blood products. Preferred methods include the steps of adding an effective, nontoxic amount of a photosensitizer such as riboflavin to the blood product and exposing the fluid to light having a peak wavelength.Type: GrantFiled: February 3, 2003Date of Patent: January 18, 2005Assignee: Gambro, Inc.Inventors: Dennis J. Hlavinka, Raymond P. Goodrich, Laura Goodrich, Daniel McGraw
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Patent number: 6825002Abstract: The present invention provides a method for changing production ratios of carotenoid compounds in a process of microbiological production of a plurality of carotenoid compounds. By controlling the concentration of dissolved oxygen in the culture during cultivation, production ratios of carotenoid compounds such as astaxanthin, adonixanthin, &bgr;-carotene, echinenone, canthaxanthin, zeaxanthin, &bgr;-cryptoxanthin, 3-hydroxyechinenone, asteroidenone and adonirubin, are changed.Type: GrantFiled: February 11, 2002Date of Patent: November 30, 2004Assignee: Nippon Mitsubishi Oil CorporationInventors: Akira Tsubokura, Haruyoshi Mizuta
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Patent number: 6824772Abstract: The invention discloses an isolated, biologically pure culture of a microorganism, Bacillus licheniformis, strain SB3086 for use as an environmentally desirable biofungicide. The invention also discloses a method for enhancing biofungicidal activity of a microbial agent. The inventional further discloses a nutrient composition comprising a microbial agent and a method for controlling plant fungal diseases and infestation of Aspergillis niger utilizing, said strain.Type: GrantFiled: January 14, 2002Date of Patent: November 30, 2004Assignee: Novozymes Biologicals, Inc.Inventors: David J. Drahos, Lee West
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Patent number: 6818211Abstract: The invention discloses a Gram-positive microorganism, Bacillus megaterium that effectively and efficiently degrades fats, oils and grease. A composition comprising said microorganism and a method for degrading fatty acids and grease are also disclosed. Availability of glycerol to the biodegrading microorganism was discovered to enhance biodegradation.Type: GrantFiled: April 23, 2002Date of Patent: November 16, 2004Assignee: Novozymes Biologicals, Inc.Inventors: Jessi Lind Tisinger, Domenic A. Paone, Jonathan Leder, David J. Drahos
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Patent number: 6812007Abstract: In a process for preparing mevinolin by fermentation of a biomass in a fermentation liquor, which includes dissolving mevinolin from the biomass into the fermentation liquor, and separating the biomass from the fermentation liquor to obtain a separated fermentation liquor, separating the mevinolin from the separated fermentation liquor, and recovering the end product, the improvement which comprises carrying out the dissolving at a pH between 7.5 and about 10, and the separating of the mevinolin is carried out at a pH between about 4.5 and about 1.Type: GrantFiled: April 19, 2000Date of Patent: November 2, 2004Inventors: Vilmos Kéri, Irma Högye, Antónia Jekkel, Ilona Bagdi, Gábor Ambrus, Attila Jakab, Attila Andor, Lajos Deák, István Szabó, János Bálint, Zsuzsanna Scheidl, Etelka Deli, Gyula Horváth, Csaba Szabó, Ildikó Láng, Imre Székely, Imre Moravcsik, Vera Kovács, Szabolcs Mátyás, Zsuzsanna Sztáray, László Eszenyi, Éva Ilköy
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Patent number: 6794175Abstract: Microorganism of the genus Eubacterium, and its obtainment and use, which is suitable in pure culture, DSM 11798, and/or mixed culture with the strain Enterococcus casseliflavus, DSM 11799, or in mixed culture with other anaerobic microorganisms for the detoxification of trichothecenes. A feedstuff additive for the inactivation of trichothecenes in feedstuffs or in the digestive tract of animals contains a pure and/or mixed culture of the microorganism (DSM 11798 or DSM 11799) or a mixed culture with other anaerobic microorganisms in an amount from 0.2 to 3 kg, in particular 0.5 to 2.5 kg, per 1000 kg of feedstuff.Type: GrantFiled: June 29, 2000Date of Patent: September 21, 2004Assignee: Erber AktiengesellschaftInventors: Eva-Maria Binder, Johann Binder
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Patent number: 6767533Abstract: A composition containing a peripheral benzodiazepine receptor ligand for topical use in the treatment of cutaneous stress.Type: GrantFiled: May 14, 2001Date of Patent: July 27, 2004Assignee: Sanofi-SynthelaboInventors: Pierre Casellas, Jean-Marie Derocq
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Patent number: 6750047Abstract: Migrastatin and a migrastatin analog can be produced by fermentation of Streptomyces platensis NRRL 18993 and used in pharmaceutical formulations to treat cancer and/or inhibit metastasis of cancer cells.Type: GrantFiled: August 17, 2001Date of Patent: June 15, 2004Assignee: Kosan Biosciences, Inc.Inventors: Chaitan Khosla, Peter Licari, John Carney
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Patent number: 6750012Abstract: Disclosed are methods of identifying psychotropic agents that do not induce motor side effects using differential gene expression. Also disclosed are novel nucleic acid sequences whose expression is differentially regulated by psychotropic agents.Type: GrantFiled: December 20, 1999Date of Patent: June 15, 2004Assignee: CuraGen CorporationInventor: Bonnie Gould-Rothberg
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Patent number: 6716617Abstract: In the fermentation process according to the invention with continuous mass cultivation of ciliates (protozoa), the ciliate cells are cultivated in a complex axenic medium—free from living feed or prey organisms—and the biomass containing the desired biogenous valuable substances is obtained by continuous (permanent) cell extraction.Type: GrantFiled: March 17, 2000Date of Patent: April 6, 2004Assignee: NutrinovaInventor: Thomas Kiy
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Patent number: 6709835Abstract: The present invention provides cell culture mediums and methods for determining levels of intracellular concentration of glutathione or cysteine in lymphocytes, indicative of nutritional status of an individual.Type: GrantFiled: December 13, 2001Date of Patent: March 23, 2004Inventor: J. Fred Crawford
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Patent number: 6686180Abstract: Methods and compositions are provided for the enhanced production of bacterial toxins in large-scale cultures. Specifically, methods and compositions for reducing bacterial toxin expression inhibitors are providing including, but not limited to, addition of toxin expression inhibitor binding compounds, culture media having reduced concentrations of toxin inhibitor metabolic precursors and genetically modified toxogenic bacteria lacking enzymes required to metabolize the toxin inhibitor metabolic precursors.Type: GrantFiled: April 4, 2001Date of Patent: February 3, 2004Assignees: Baxter International Inc., Baxter Healthcare S.A.Inventors: Milan S. Blake, John A. Bogdan, Jr., Javier Nazario-Larrieu
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Patent number: 6682914Abstract: Newly-isolated and purified metabolites which are effective in regulating the development of at least one filamentous fungal microorganism species are disclosed. These compounds, which are referred to as conidiogenol and conidiogenone, may be used to induce conidiation in and/or to inhibit the growth of populations of such fungal species. The compounds are preferably produced by culture of the fungal species Penicillium cyclopium, and may be subsequently recovered from the culture medium and purified. Methods of using and methods of producing these compounds are also disclosed.Type: GrantFiled: October 31, 2001Date of Patent: January 27, 2004Assignee: Universidad del Pais Vasco/Euskal Herriko UnibersitateaInventors: Unai Ugalde, Tomas Roncal, Olov Sterner
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Patent number: 6677149Abstract: A method for preserving immobilized or unimmobilized microbial cells having nitrilase activity and for stabilizing the nitrilase activity of unimmobilized or immobilized microbial cells has been developed. Aqueous suspensions containing at least 100 mM bicarbonate, carbonate, or carbamate salts limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), Acidovorax facilis 72W (ATCC 55746), and transformed microbial cells having nitrilase activity, the host cells transformed with Acidovorax facilis 72W nitrilase activity. Especially preferred is an embodiment using ammonium carbamate as the inorganic salt.Type: GrantFiled: May 14, 2001Date of Patent: January 13, 2004Assignee: E. I. du Pont de Nemours and CompanyInventors: Robert Dicosimo, Arie Ben-Bassat, Robert D. Fallon
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Patent number: 6664095Abstract: The present invention provides an improved solid state fermentation device that combines all of the operations of microorganism cultivation (sterilization, inoculation, cultivation, extraction, and post extraction treatment). This solid state fermentation device is modular in nature and operates in a contained manner so that the live microorganisms from the reactor cannot come into contact with the environment and pollute the environment and also so that the environment inside the bioreactor is aseptic. Another aspect of this invention allows fermentation of microorganisms without inhibiting the growth of the microorganism. Specifically, the bioreactor is designed to remove heat that accumulates inside the bioreactor during fermentation by conduction. Additionally, there is a mechanism to add fluid to the interior of the bioreactor that permits equal distribution and precise control of a variety of environmental parameters.Type: GrantFiled: May 5, 2000Date of Patent: December 16, 2003Assignee: Biocon India LimitedInventors: Shrikumar Suryanarayan, Kiran Mazumdar
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Patent number: 6653135Abstract: An assay to determine the specific expression and suppression of proteins in response to a stressor is disclosed. An organism exposed to a stressor, including disease caused by exposure to, e.g., a parasite, or a substance suspected of causing an adverse effect, is assayed to determine a first set of proteins expressed and a second set of proteins suppressed in response to the stressor. The amount of each protein expressed and the amount of each protein suppressed can be statistically analyzed to determine which proteins are most useful in diagnosing the stressor. A protein profile for a first stressor can be compared to protein profiles for a second stressor, a third stressor, etc. A distinct protein expression signature (PES) for the first stressor can be identified by determining subsets fo proteins expressed and/or suppressed only in response to the first stressor.Type: GrantFiled: September 13, 2000Date of Patent: November 25, 2003Assignee: University of MarylandInventor: Brian P. Bradley
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Patent number: 6649401Abstract: The invention discloses a Gram-positive microorganism, Bacillus megaterium, that effectively and efficiently degrades fats, oils and grease. A composition comprising said microorganism and a method for degrading fatty acids and grease are also disclosed.Type: GrantFiled: May 18, 2001Date of Patent: November 18, 2003Inventors: Jessi Lind Tisinger, David Joseph Drahos, Jonathan Leder, Domenic Anthony Paone
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Patent number: 6646185Abstract: The present invention provides an efficient genetic transformation system for the zoysiagrass plant (Zoysia japonica Steud.). Also provided are optimized media compositions and culture conditions for the zoysiagrass transformation. The reliable transformation system for zoysiagrass was developed by optimizing several factors that significantly affect calli growth and plant regeneration. Callus type and co-cultivation period absolutely influenced the transformation efficiency. Concentrations of 2,4-D, CaCl2 and acetosyringone were also critical factors. The best result was achieved when type 3 calli were co-cultivated on a 2,4-D-free co-cultivation medium for 6 days. Removal of calcium and addition of 50 mg/liter acetosyringone during co-cultivation drastically enhanced the efficiency. The invention also provides a bialaphos-resistant zoysiagrass plant, which can be used in golf courses and athletic fields to save the maintenance cost.Type: GrantFiled: November 20, 2001Date of Patent: November 11, 2003Assignee: Korea Kumho Petrochemical Co., Ltd.Inventors: Tohyama Kohichi, Chang-Hyu Bae, Jeong-Gu Kang, Pill-Soon Song, Chung-Mo Park, Hyo Yeon Lee
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Patent number: 6632649Abstract: The growth of the bacterium, Burkholderia cepacia (ex Pseudomonas cepacia) CIP 1-2052 is substantially increased when it is supplied with tert-butanol (TBA) or tert-amyl alcohol (TAA) as the sole source of carbon and energy in the presence of at least one cobalt salt, at a concentration of 0.01 to 4 mg/l of medium. The bacterium is degraded to CO2 in the presence of oxygen. The inoculom produced by the process and the degradation process for these alcohols are useful for water treatment applications.Type: GrantFiled: November 8, 2000Date of Patent: October 14, 2003Assignee: Institut Francais du PetroleInventors: Pascal Pivetau, Françoise Fayolle, Frédéric Monot
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Patent number: 6617126Abstract: The present invention relates to a method for improving the growth and detection of bacteria, yeasts, fungi or cocci, by adding sterile-filtered yeast extract and/or p-iodonitrotetrazolium violet to the culture medium.Type: GrantFiled: September 28, 2000Date of Patent: September 9, 2003Assignee: Biotest AGInventor: Jürgen Horn
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Patent number: 6610530Abstract: A method of enhancing biomass yield of a lactic acid bacterial species cell culture, comprising cultivating the cells in a process comprising the steps of providing conditions that results in a reduced glycolytic flux and providing conditions that enable the cells to have, under aerobic conditions, a respiratory metabolism. The increased yield of biomass may be the result of an increased yield of ATP which can be obtained by activating the native ATP synthase activity of the H+-ATPase complex by lowering the ATP/ADP ratio, e.g. by carbon source limitation, and/or by increasing the proton gradient (membrane potential) of the cells, e.g. by enhancing or establishing an electron transport chain which can be achieved by enhancing expression of dehydrogenases or electron transport chain components, by adding to the medium a quinone or porphyrin compound or by enhancing the expression of the H+-ATPase activity.Type: GrantFiled: July 5, 2001Date of Patent: August 26, 2003Assignee: Danmarks Tekniske UniversitetInventors: Lars Blank, Peter Ruhdal Jensen, Jensen B. Koebmann
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Patent number: 6599733Abstract: A biodegradation process for the organophosphonate product of Sarin (O-isopropyl methylphosphonofluoridate) hydrolysis, i.e., isopropylmethylphosphonate (IMPA). This process provides a feasible biodegradation demilitarization alternative to Sarin incineration. Public opposition of nerve agent incineration is widespread, and alternative methods are sought to help the U.S. Army meet the 2007 demilitarization deadline imposed by the Chemical Weapons Convention. This process uses a two-step approach to IMPA biodegradation. In the first step, a concentrated IMPA solution is used as the sole nutritional carbon and phosphorus source for microbial cultures. The second step involves diluting the culture and adding an inexpensive carbon source to encourage bacterial phosphate assimilation. The biodegradation typically involves a consortium of microorganisms comprising Methylobacterium radiotolerans GB21, Agrobacterium tumefaciens GB2GA, Klebsiella oxytoca GB2CS, GB272, Aureobacterium sp.Type: GrantFiled: April 27, 2001Date of Patent: July 29, 2003Assignee: The United States of America as represented by the Secretary of the ArmyInventors: Ilona J. Fry, Joseph J. DeFrank, James P. Earley
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Publication number: 20030134405Abstract: A medium for culturing microorganisms includes at least one compound which can be used as a source of phosphorus for microorganisms but which at least one compound will not precipitate Pb2+ ions, so that the medium does not form a precipitate in the presence of up to about 25 mM Pb2+ ions. Preferred compounds are O-phospho-L-amino acids. A buffer may be included in the medium. The medium is particularly well suited for remediating lead contaminated site by bioremediation.Type: ApplicationFiled: November 14, 2002Publication date: July 17, 2003Inventors: Harry J. Guttman, James A. Ryan, Wendy Jo Davis-Hoover
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Patent number: 6589771Abstract: A method for arousing dormant bacteria. The method comprises inducing diffusion of intracellular solutes from dormant bacteria and then allowing an adjustment period for a length of time sufficient to initiate arousal. The decrease in intracellular osmolality or pH can be induced by methods such as extraction, dilution, or dialysis. The method has been standardized using Dulbecco's phosphate buffered saline as the solution. The aroused bacteria can then be selected or recovered by growing them on media for a period of time. If the adjustment period is prolonged, many bacteria can become hypermutative.Type: GrantFiled: October 28, 1999Date of Patent: July 8, 2003Assignee: Immunom Technologies, Inc.Inventor: William E. Marshall
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Patent number: 6582939Abstract: The preparation of alkaline earth salts of D-pantothenic acid, in which the fermentation is carried out in the presence of alkaline earth compounds.Type: GrantFiled: October 12, 2000Date of Patent: June 24, 2003Assignee: Degussa AGInventors: Michael Binder, Walter Pfefferle, Ulrich Becker, IIona Walger, Klaus Erich Uffmann, Georg Thierbach, Achim Marx, Thomas Hermann
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Patent number: 6558941Abstract: Nanoemulsions are prepared containing oily droplets of a diameter of less than 100 nm in an aqueous phase. The oily droplets contain a lipophilic substance, and have on their surface an amphoteric emulsifier in an amount of preferably 0.65 to 0.75 parts by weight amphoteric emulsifier for one part by weight of oily component forming the droplets. The oily component of the droplets is preferably a triglyceride containing oleic acid and/or linoleic acid. The nanoemulsions, which may be sterile, are used for delivering into cells the lipophilic substance contained by the oily droplets. This delivery can be used to biotransform the lipophilic substance, promote cell differentiation, growth or biosynthesis of a desired substance, or to test for toxicity of the lipophilic substance.Type: GrantFiled: June 29, 2001Date of Patent: May 6, 2003Assignee: Mibelle AG CosmeticsInventors: Fred Zuelli, Franz Suter
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Patent number: 6555356Abstract: A method and microbial culture medium for inhibiting homoserine lactone and/or acylated homoserine lactone regulated processes in microorganisms using furanone compounds.Type: GrantFiled: March 4, 1998Date of Patent: April 29, 2003Assignee: Unisearch LimitedInventors: Staffan Kjelleberg, Peter David Steinberg, Peter Canisius De Nys, Ria Maximilien, Michael Manefield, Michael Givskov, Lone Gram
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Publication number: 20030049338Abstract: The invention relates to a nitrogenous composition resulting from the enzymatic hydrolysis of an aqueous solution of maize gluten, having a ratio of the concentrations of inorganic phosphorus to total phosphorus (Pi/Pt) greater than or equal to 0.05, preferably from 0.05 to 0.5 and a ratio of the concentrations of amine nitrogen to total nitrogen (Na/Nt) greater than or equal to 0.025. The invention also relates to the use of a nitrogenous composition according to the invention in culture media for microorganisms which produce, in particular, organic acid.Type: ApplicationFiled: September 23, 2002Publication date: March 13, 2003Inventors: Catherine Fouache, Laurent Seigueilha, Eric Dubois
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Patent number: 6524839Abstract: Treatment of cells with lower alcohol provides cells having the reaction rate 350 to 600 times higher than that of cells that are not treated with lower alcohol More specifically, a simple operation of treating cells with lower alcohol provides a catalyst that has a higher activity that that of a cell extract and can be recycled.Type: GrantFiled: March 12, 2001Date of Patent: February 25, 2003Assignee: Kansai Chemical Engineering Co., Ltd.Inventors: Hideki Fukuda, Akihiko Kondo
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Patent number: 6521405Abstract: The invention relates to improvements in cell-free systems for initiating DNA replication under cell cycle control. The system comprises a synchronous population of G1 nuclei which have been released from G0; and S phase cytosol. In a preferred aspect, a polypeptide is supplied to the system, wherein the polypeptide is Cdc6 and/or at least one MCM protein. The system is suitable for DNA replication assays, for example to test substances which modulate DNA replication.Type: GrantFiled: March 9, 2000Date of Patent: February 18, 2003Assignee: Cancer Research Campaign Technology LimitedInventors: Ronald Laskey, Gareth Haydn Williams, Torsten Krude, Kal Stoeber, Anthony David Mills
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Publication number: 20020162794Abstract: A chemical additive is taught for the improvement of anaerobic digestion treatment of water-borne organic biomass by the addition of at least about 500 ppm or greater of a formate salt or mixtures of formate salts, the added presence being based on the total solids content of the organic based biomass in the wastewater stream, with the formate salts enhancing the anaerobic microbial activity in an anaerobic digestion zone and thereby promoting reduction of residual sludge of the biomass and reduction of chemical oxygen demand for the biomass digestion. The formate salts can be added by mixing the soluble salts with the water-borne organic biomass, or in the alternative by producing the formate salts insitu by adding formic acid to the biomass in the presence of at least about 100 mg/:based on the total biomass solids content followed by addition of appropriate amounts of base materials to complete the formation of the dissociated salts so as to maintain the wastewater in the appropriate pH range.Type: ApplicationFiled: February 27, 2001Publication date: November 7, 2002Inventor: Richard William Tock
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Patent number: 6468782Abstract: This invention provides methods of drying and stabilizing prokaryotic cells, and the compositions obtained thereby. The cells are first cultured or incubated under conditions sufficient to induce intracellular trehalose, suspended in a stabilizing solution and dried to form a solid glass. The resulting product is storage-stable at room temperature, showing little viability loss on storage.Type: GrantFiled: December 4, 1997Date of Patent: October 22, 2002Assignee: Quadrant Healthcare (UK) LimitedInventors: Alan G. Tunnacliffe, David T. Welsh, Bruce J. Roser, Kamaljit S. Dhaliwal, Camilo Colaco
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Patent number: 6465221Abstract: The present invention relates to a method of producing a taxane-type diterpene wherein a cell and/or a tissue of a plant which produces the taxane-type diterpene is cultured in the presence of at least one substance selected from the group consisting of coronatines, a bacterium which produces the coronatines, a culture solution or a culture extract of such bacterium, cyclic polysaccharides, fatty acids, and an imino or amino derivative of jasmonic acids, then the taxane-type diterpene is recovered from the resulting cultures.Type: GrantFiled: March 19, 2001Date of Patent: October 15, 2002Assignee: Mitsui Chemicals, Inc.Inventors: Yukihito Yukimune, Yasuhiro Hara, Hiroaki Tan, Ikuo Tomino
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Patent number: 6458581Abstract: The present invention provides a method for carrying out in vitro the complete developmental sequence culture of tissular parasites, which includes culturing the parasites in a totally defined culture medium which is an axenic monophasic liquid culture medium, free of serum and free of serum-derived or cell-derived macromolecules, proteins and peptides. For obtaining amastigote forms, this medium is buffered at a pH of 5.5 to 6.5 and has an osmolarity of at least 400 milliosmoles/kg of liquid. For obtaining promastigote forms, the medium is buffered at a pH of 7 to 7.5 and has an osmolarity of at least 300 milliosmoles/kg liquid. Application to the in vitro culture of different stages of tissular parasites such as Leishmania, T. cruzi, and hamatoprotozoa is also provided.Type: GrantFiled: November 9, 1995Date of Patent: October 1, 2002Assignee: Institut francais de recherche scientifique pour le developpement en Cooperation (ORSTOM)Inventor: Jean-Loup Lemesre
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Publication number: 20020127700Abstract: Media for growth enhancement and resuscitation of mycobacteria (such as Mycobacterium tuberculosis, Mycobacterium paratuberculosis, and Mycobacterium leprae) are provided. The media comprise isolated cell extract, early-stationary-phase or stationary phase supernatant, or a substantially purified component thereof such as a protein, a peptide fragment of the protein, or a phospholipid. The protein is Rv1147c and the phospholipid or a component of a phospholipid. Diagnostic methods and kits utilizing the media are also provided, as well as treatment methods utilizing spent culture supernatant and cell extracts, or components thereof.Type: ApplicationFiled: December 7, 2001Publication date: September 12, 2002Inventor: Ying Zhang
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Patent number: 6448019Abstract: The invention features screening methods which can be used to identify agents, known as vasoprotective agents, which inhibit vascular smooth muscle cell activation and/or proliferation or enhance vascular endothelial cell activation and/or proliferation or activate estrogen responsive genes in vascular cells. Preferred vasoprotective agents are relatively vasospecific, i.e., their effect on one or more types of vascular cells is more pronounced than their effect on other cell types. Treatment with such vasospecific agents will generally be associated with fewer undesirable side-effects than treatment with estrogen. The methods of the invention are screening assays in which candidate agents are examined to identify vasoprotective agents. One type of screening assay involves examining the effect of a candidate agent on cell proliferation and/or cell activation. Another type of screening assay involves examining the effect of a candidate agent on the expression of a gene which is responsive to estrogen.Type: GrantFiled: October 1, 1999Date of Patent: September 10, 2002Assignee: New England Medical Center Hospitals, Inc.Inventors: Michael E. Mendelsohn, Richard H. Karas
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Patent number: 6432883Abstract: Methods for increasing the resistance of a plant to the effects of plant stress utilizing glycolic acid, a salt thereof, or a mixture thereof, are described. Methods for stimulating plant growth utilizing an ammonium salt of glycolic acid are also described. Further described are methods for stimulating microbial growth utilizing selected amounts of glycolic acid, a salt thereof, or a mixture thereof.Type: GrantFiled: April 20, 2000Date of Patent: August 13, 2002Assignee: Emerald BioAgriculture CorporationInventor: Alan M. Kinnersley
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Patent number: 6410282Abstract: The invention relates to a method for enhancing levels of polyunsaturated fatty acid levels in thraustochytrid fungi, using media supplemented with polyvinyl pyrrolidone (PVP) to increase viscosity and which comprises: (a) providing a thraustochytrid fungus strain NIO-TH 21, corresponding to the species Ulkenia radiata Gaertner, being the culture with Accession No. AB22115 of the National Institute of Bioscience and Human-Technology, Japan; (b) inoculating the above said strain in a culture medium; (c) growing the culture for 2 days at a temperature ranging from 25 to 30° C.; (d) obtaining the cultures for use as innoculum using the above said medium to inoculate a medium with different concentrations of polyvinyl pyrrolidone (PVP); (e) growing the culture separately for 2 to 5 days at a temperature ranging from 25 to 30° C.; and (f) harvesting the cells from the above culture by centrifugation and extracting the enhanced amounts of docosahexaenoic acid (DHA) and eicosapentaenoic acids (EPA).Type: GrantFiled: March 30, 2000Date of Patent: June 25, 2002Assignee: Council of Scientific and Industrial ResearchInventors: Seshagiri Raghu Kumar, Dorai Rajasingam Chandramohan, Ehrlich Desa
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Patent number: 6403343Abstract: This invention relates to a method of producing a taxane-type diterpene(s) wherein tissues or cells of a plant which produces taxane-type diterpene(s) is cultured in the presence of at least one selected from the group consisting of jasmonic acids, compounds containing a heavy metal, complex ions containing a heavy metal, heavy metal ions, amines and antiethylene agents, a method of producing a taxane-type diterpene wherein the tissues or the cells of the plant are cultured by controlling the oxygen concentration in a gas phase in a culture vessel to less than the oxygen concentration in the atmosphere from the initial stage of the culture, or by controlling the dissolved oxygen concentration in a fluid medium which is in contact with the tissue or the cell to less than the saturated dissolved oxygen concentration at that temperature from the initial stage of the culture, a method of producing a taxane-type diterpene wherein the tissue or the cell of the plant is cultured in a culture vessel, while oxygenic gType: GrantFiled: April 28, 1999Date of Patent: June 11, 2002Assignee: Mitsui Chemicals, Inc.Inventors: Yukihito Yukimune, Yasuhiro Hara, Yosuke Higashi, Naoto Ohnishi, Homare Tabata, Chuzo Suga, Kouichi Matsubara
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Patent number: 6387687Abstract: A microorganism having indolmycin-producing ability and tryptophan analogue resistance is provided, and indolmycin or its salt can be produced efficiently by cultivating such microorganism in a medium to produce and accumulate indolmycin or its salt in the culture broth followed by recovering a product.Type: GrantFiled: January 23, 2001Date of Patent: May 14, 2002Assignee: Takeda Chemical Industries, Ltd.Inventors: Akihiko Naotsuka, Motoo Izawa, Ken-ichiro Miyagawa
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Patent number: 6379918Abstract: Compositions, formulae, devices and methods for the detection of target microorganisms, such as by visual immunoprecipitate assay, enzyme linked immunoassay, chemiluminescence, immunoblotting, or similar detection technology, wherein detection requires the discrimination among closely related genera, species and strains of antigenically related microorganisms based on immunological reactivity of a highly conserved antigen epitopes with a reagent system comprised of an antibody linked to a detecting reagent. The invention permits a detectable event to occur by exposing inaccessible but highly conserved and specific antigen epitopes to the detecting reagent. Exposure of such antigen epitopes without inactivating microbial metabolism allows for specific detection.Type: GrantFiled: August 13, 1999Date of Patent: April 30, 2002Assignee: Biocontrol Systems, Inc.Inventors: Philip T. Feldsine, David E. Kerr, Ping Zhu, Linda Mui
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Patent number: 6368847Abstract: A composition for recovery and enumeration of Campylobacter species that includes a selective Campylobacter medium plus an indicator, 2,3,5-triphenyltetrazolium chloride. Improved selective media including the indicator, contains a nutrient medium with an energy source, agar, a reducing agent, and selective agents. The selective agents are a mixture of agents selected from cycloheximide, cefoperazone, vancomycin, trimethoprim, polymyxin B; rifampicin, amphotericin, cefoperazone, vancomycin, trimethoprim, and nystatin. The salts of these agents are also useful.Type: GrantFiled: June 1, 2000Date of Patent: April 9, 2002Assignee: The United States of America, as represented by the Secretary of AgricultureInventors: John Eric Line, Johnna Kennedy Garrish, Kirsten Elizabeth Glassmoyer Pearson
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Publication number: 20020039781Abstract: A fermentation process post-sterile additive device and method of operation is detailed. The device is used to deliver additive as a heavy-drop mist. The mist covers the entire fermenting batch head surface of the vat broth. This improves mixing and reduces the amount of additive to appropriate introduction levels throughout log phase fermentation, preventing coating of cultivating cells and interference with cell respiration. A steady state growth rate is fostered.Type: ApplicationFiled: December 4, 2001Publication date: April 4, 2002Applicant: ICOS CorporationInventor: Drew C. Coleman
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Publication number: 20020028445Abstract: The invention relates to a method and device for detecting the toxic and mutagenic effect of chemicals and mixtures of substances using immobilized luminescent bacteria or microorganisms that have been rendered bioluminescent by the transfer of adequate vector plasmids, which are provided with an optoelectronic component for photometry in such a way that a biosensor is formed. Since the detection of biological effects can take place during a long period of time, the invention makes it possible to carry out surveillance tasks in the environment and to conduct process controls in agriculture and industry.Type: ApplicationFiled: April 15, 1999Publication date: March 7, 2002Inventor: HORST BERCHER
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Patent number: 6348346Abstract: A method for inhibiting culture agglutination in a fermentation medium including immunoglobulins includes the step of treating a source of immunoglobulins with an enzyme, such as papain, ficin, bromelain and mixtures thereof to hydrolyze the immunoglobulins into immunoreactive peptides having a molecular weight of less than 10,000 Daltons. This is accomplished utilizing ultrafiltration and/or diafiltration techniques. Next, is the collecting of peptides so produced and the adding of the peptides to a growth media for microorganisms useful in fermenting the fermentation medium. This is followed by growing the microorganisms in the inoculated growth media and adding the microorganisms grown in the treated growth media to the fermentation medium. A method for inhibiting the binding activity of mammalian immunoglobulins with an immunogen and inhibitors of the binding activity of immunoglobulins are also disclosed and claimed.Type: GrantFiled: February 13, 1997Date of Patent: February 19, 2002Assignee: University of Kentucky Research FoundationInventors: Clair L. Hicks, Zeynep Ustunol