Abstract: The chemical modification of virulent Pasteurella multocida and Pasteurella haemolytica strains and preparation of live bacteria vaccines from the modified organisms for immunization of bovine, porcine and ovine animal species are disclosed.

Abstract: A process for producing polysaccharide consisting of a partially acetylated variable block copolymer of D-mannuronic and L-guluronic acid residues comprises growing a biologically pure culture of a Pseudomonas mendocina microorganism selected from the group consisting of NCIB 11687, 11688, and 11689 in an aqueous nutrient medium by submerged aerobic fermentation of an assimilable carbon source and recovering the polysaccharide. Biologically pure cultures of the organisms are another feature of the invention.

Abstract: This invention provides a continuous bioconversion process in which a cross-flow membrane filtration zone is employed to recover a whole cell-containing retentate stream and a cell-free bioconversion product-containing permeate stream. The retentate stream is recycled to the fermentation zone. In a specific embodiment, toluene is bio-oxidized to muconic acid with a microorganism such as Pseudomonas putida Biotype A strain ATCC 31,916. The muconic acid is recovered as a precipitate from the cell-free permeate fermentation broth, and the fermentation broth is recycled in the process.

Abstract: A method and materials are provided for replication of virulent Treponema pallidum in tissue culture, employing a modified Eagle's minimum essential medium, wherein said in vitro cultivated T. pallidum can be utilized as a source of relatively pure organisms, free of host tissue, for the preparation of a vaccine against syphilis and as a source of organisms for use in specific immunological tests for syphilis.

Abstract: Microbiological oxidations of organic compounds including C6 to C28 alkanes, C2 to C18 alkenes and cyclic compounds such as cyclohexane and benzene carried out using as catalysts methane-utilizing bacteria adapted to utilize methanol as a carbon source. The application also covers methane-utilizing bacteria adapted to utilize methanol as a carbon source and a method for producing such bacteria. In the method methane-utilizing bacteria are cultured in the presence of methanol vapor as principal carbon source for sufficient time to allow adaptation to occur.

Abstract: Lactobacillus and Streptococcus are cultured by special process steps to derive by controlled transmutation a strain of organisms having high tolerance to acidity above 1.5%, and are tolerant to metallic salts such as cobalt carbonate which tend to poison such organisms by limiting growth. The organisms are cultured in a transfer process from starter organisms that tend to clump in the presence of metallic ions to develop the improved strain which does not clump when cultured in the presence of metallic salts thereby permitting increased production. A characterizing feature of the resulting transmuted organisms therefore is the freedom of a tendency to clump in the presence of the cobalt ion, a feature uncharacteristic of the starting organisms. The organisms are useful for enhancing animal and plant growth.

Abstract: Osmotolerant yeasts having high activity in sweet doughs are obtained in high yield by incremental addition or feeding of salts such as NaCl, KCl, CaCl.sub.2, MgCl.sub.2, Na.sub.2 SO.sub.4, MgSO.sub.4, and K.sub.2 SO.sub.4 in the latter propagative stages of yeast production.

Abstract: A process for the production of a storage-stable preparation of micro-organisms, starting from a mixed culture of micro-organisms, which is capable to degrade products of industrial organic syntheses, which comprises that the culture conditions of an active mixed culture enriched in the customary manner are optimized, with the addition of the product to be degraded, in the customary manner according to the rate of degradation of the product and this mixed culture is converted in at least two passes into a stabilized activated mixed culture under the optimum conditions thus determined, and the culture thereby obtained is rendered storage-stable, its activity being maintained.

Abstract: Deacidifying wine by passage through an alginate gel containing living cells of Leuconostoc oenos therein. To ensure maximum viability, the alginate gel is stored in a resting medium, preferably sterile grape juice containing 5-12% ethanol. Before deacidifying wine the immobilized cells are conditioned to a wine milieu.

Abstract: Acetic acid has been prepared by a fermentation process using Clostridium thermoaceticum in a varied form obtained by repeated fermentations under anaerobic conditions and low redox potentials at successively more acidic pH's than the original organism.

Abstract: Lactobacillus acidophilus is cultured by special process steps to derive a strain of organisms having a length of over ten microns and a tough stable outer hide, and which produce high acidity above 1.5%, exhibit a characteristic odor, and are tolerant to metallic salts such as cobalt carbonate. They are cultured in a transfer process from starter organisms of different characteristics to develop the improved strain which retains its characteristics in the presence of metallic salts added to the nutrient before incubation and are free of a tendency to clump.

Abstract: Fermentation of acid-containing dough in bread-making is carried out with a baker's yeast in the form of compressed fresh yeast or dried yeast that has reduced inhibition to acid in the dough. The yeast is preferably prepared by selecting a strain of quick yeast, adapted to maltose and stable on conversation and drying, and cultivating the yeast by a process wherein during a last discontinuous cycle of multiplication of the yeast, a discontinuous flow of molasses is carried out by brief interruptions of flow.

Abstract: The present invention relates to deodorization of excrement by Lactobacillus strains in cultivation of said strains with S-, N- or C-compounds which are odoriferous components of said excrement and/or certain amino acids.

Abstract: A dry yeast composition in particulate form containing at least 92% dry matter is prepared consisting essentially of active dry bakers' yeast capable of fermenting sweetened doughs containing more than 5% sugar and an emulsifying agent having an HLB value of between 3 and 11. The emulsifying agent is added to the yeast before drying and protects the yeast during drying.

Abstract: A process for the production of baker's yeast with improved activity or leavening power under acid leavening conditions is carried out by cultivating fresh yeast conventionally to the last propagation stage, propagating the fresh yeast in the last propagation stage in the presence of from 0.1 gm to 10 gm per liter of culture broth containing a source of carbon and nitrogen, of an aliphatic carboxylic acid having from 2 to 4 carbon atoms, and recovering baker's yeast with improved activity or leavening power.

Abstract: Incubation of a parent strain of Vibrio cholerae at elevated temperature to produce a hypotoxinogenic variant strain and mutation of the variant strain results in mutant strains which retain the biotype and antigens of the parent strain, and which are genetically stable and useful as live oral vaccines for immunization against cholera. Preferably, a hypotoxinogenic mutant which is also non-pathogenic in animal model systems is isolated by the method disclosed in this application.

Abstract: A process for culturing methanol-utilizing yeasts, wherein a methanol-utilizing yeast is cultured in a medium containing methanol as a main carbon source, using as seeds yeast cells obtained by culturing the yeast in a culture liquor containing methanol at a concentration higher than 0.1 wt. % and not more than 6 wt. %.

Abstract: The present invention is directed to a process for biological denitrification of effluents, containing up to 10 g/l of nitric nitrogen, which comprises using an active denitrification support made of selected high performance denitrifying bacteria fixed on a neutral support.

Abstract: A method for the inactivation of microbial toxins and attenuation of vaccines by treating the toxin or vaccine with a tannin, especially a condensed tannin derived from fruit of the genus Diospyros, particularly the persimmon, without loss of the desired antigenicity.