Abstract: Testosteronan, a heparosan analog having the structure [-4-D-GlcUA-?1,4-D-GlcNAc-?1-]n, is produced by testosteronan synthase, a single protein that is a dual-action catalyst that utilizes UDP-GlcUA and UDP-GlcNAc to synthesize a polysaccharide having the structure [-4-D-GlcUA-?1,4-D-GlcNAc-?1-]n.
Type:
Application
Filed:
January 31, 2012
Publication date:
June 5, 2014
Applicant:
The Board of Regents of the University of Oklahoma
Abstract: Monospecific and bispecific EGFR and/or c-Met FN3 domain containing molecules, isolated nucleotides encoding the molecules, vectors, host cells, and methods of making thereof are useful in the generation of therapeutic molecules and treatment and diagnosis of diseases and disorders.
Type:
Application
Filed:
November 21, 2013
Publication date:
June 5, 2014
Applicant:
Janssen Biotech, Inc.
Inventors:
Anderson Mark, Ricardo Attar, Michael Diem, Linus Hyun, Steven Jacobs, Alastair King, Donna Klein, Sheri Moores, Karyn O'Neil, Kristen Picha
Abstract: Monospecific and bispecific EGFR and/or c-Met FN3 domain containing molecules, isolated nucleotides encoding the molecules, vectors, host cells, and methods of making thereof are useful in the generation of therapeutic molecules and treatment and diagnosis of diseases and disorders.
Type:
Application
Filed:
November 20, 2013
Publication date:
June 5, 2014
Applicant:
Janssen Biotech, Inc.
Inventors:
Anderson Mark, Ricardo Attar, Michael Diem, Linus Hyun, Steven Jacobs, Alastair King, Donna Klein, Sheri Moores, Karyn O'Neil, Kristen Picha
Abstract: Novel materials and methods useful for expressing heterologous proteins in prokaryotic cells are provided, including prokaryotic cells expressing FkpA and/or Skp.
Type:
Application
Filed:
February 3, 2012
Publication date:
June 5, 2014
Inventors:
Raphael D. Levy, Chung-Leung Chan, Kiranjit K. Ahluwalia, Toshihiko Takeuchi
Abstract: The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof.
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains.
Type:
Application
Filed:
April 26, 2012
Publication date:
May 29, 2014
Applicant:
Novozymes, Inc.
Inventors:
Nikolaj Spodsberg, Brett McBrayer, Pierre Cassland
Abstract: The present invention relates to variants of alpha amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Application
Filed:
July 6, 2012
Publication date:
May 29, 2014
Applicant:
NOVOZYMES A/S
Inventors:
Tomoko Matsui, Aki Tomiki, Guillermo Coward-Kelly
Abstract: The present invention relates to polypeptides that bind to H3K4 methylated chromatin, and in particular to the use of reagents comprising such polypeptides for epigenetic/epigenomic analysis.
Type:
Application
Filed:
March 28, 2012
Publication date:
May 29, 2014
Applicant:
UNIVERSITY OF OSLO
Inventors:
Reidunn B. Aalen, Tage Thorstensen, Rein Aasland, Verena Hoppmann
Abstract: The present invention relates to novel Varicella-zoster virus strain and to a chicken pox and herpes zoster virus vaccine using the same. More particularly, the present invention relates to genome DNA of VZV MAV/06 strain isolated from a Korean patient, to an open reading frame (ORF) thereof, to a protein coded by the genome DNA of VZV MAV/06 strain and the ORF thereof, and to a vaccine composition which contains the protein as an active ingredient.
Type:
Application
Filed:
February 24, 2012
Publication date:
May 29, 2014
Applicant:
MOGAM BIOTECHNOLOGY RESEARCH INSTITUTE
Inventors:
Geun Hee Kim, Shi Nae Kwon, Chan Hee Lee, Yeop Yoon
Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.
Type:
Grant
Filed:
October 26, 2012
Date of Patent:
May 27, 2014
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Abstract: The invention provides means and methods for producing one or more Ig-like molecules in a single host cell. Novel CH3 mutations enabling the production of monospecific and/or bispecific Ig-like molecules of interest are also provided.
Type:
Application
Filed:
November 15, 2013
Publication date:
May 22, 2014
Applicant:
MERUS B.V.
Inventors:
Cornelis A. DE KRUIF, Linda Johanna Aleida HENDRIKS, Ton LOGTENBERG
Abstract: The present disclosure provides antibodies that bind complement C1s protein; and nucleic acid molecules that encode such antibodies. The present disclosure also provides compositions comprising such antibodies, and methods to produce and use such antibodies, nucleic acid molecules, and compositions.
Type:
Application
Filed:
November 1, 2013
Publication date:
May 22, 2014
Inventors:
Peter Van Vlasselaer, Graham Parry, Nancy E. Stagliano, Sandip Panicker
Abstract: The invention provides a process for reacting a carboxylic acid ester of the formula (I) R1-A-COOR2 ??(I), wherein R1 is hydrogen, —CH2OH, —CHO, —COOR3, —CH2SH, —CH2OR3 or —CH2NH2, R2 is an alkyl group,R3 is hydrogen or an alkyl group, and A is a substituted, unsubstituted, linear, branched and/or cyclic alkylene, alkenylene, arylene or aralkylene radical having at least 4 carbons,in the presence of a cell. The process comprises a) contacting the cell with said carboxylic acid ester in an aqueous solution,wherein the cell is a recombinant cell which has reduced activity of a polypeptide comprising SEQ ID NO: 2 or a variant thereof over the wild-type cell.
Abstract: This document describes biochemical pathways for producing butadiene by forming two vinyl groups in a butadiene synthesis substrate. These pathways described herein rely on enzymes such as mevalonate diphosphate decarboxylase, isoprene synthase, and dehydratases for the final enzymatic step.
Type:
Application
Filed:
June 12, 2013
Publication date:
May 22, 2014
Applicant:
INVISTA North America S.á r.l.
Inventors:
Paul S. Pearlman, Changlin Chen, Adriana Leonora Botes, Alex Van Eck Conradie
Abstract: The present invention relates to variants of a parent alpha-amylase. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Type:
Application
Filed:
June 29, 2012
Publication date:
May 22, 2014
Inventors:
Svend Kaasgaard, Jens Oebro, Signe Eskildsen Larsen, Allan Svendsen, Annette Helle Johansen, Michael Skjoet, Carsten Andersen, Lars Beier, Esben Peter Friis, Miguel Duarte Guilherme Pereira Toscano, Mads Bjoernvad, Frank Winther Rasmussen, Liv Spaanger Christiansen
Abstract: The present disclosure relates to methods of controlling the sulfide (S2˜) content in systems, such as oil and gas reservoirs and pipelines, by the use of chlorine oxyanions and microorganisms with (per)chlorate-reducing activity.
Type:
Application
Filed:
May 31, 2012
Publication date:
May 15, 2014
Applicant:
The Regents of the University of California
Abstract: The present invention provides novel mammalian alpha-kinase proteins: melanoma alpha-kinase (MK), heart alpha-kinase (HK), kidney alpha-kinase (KK), skeletal muscle alpha-kinase (SK), and lymphocyte alpha-kinase (LK). In particular, a novel kinase type is herein provided, characterized by the presence of an alpha-kinase catalytic domain and an ion channel domain. Isolated nucleic acids of the alpha-kinases MK, HK, KK, SK and LK are provided. Methods for making the novel alpha-kinases, cells that express the alpha-kinases and methods for treating an animal in need of either increased or decreased activity of the alpha-kinases are provided.
Abstract: The present invention relates to anti-IL-1 beta binding members and in particular to monovalent high potency IL-1 beta-binding antibody fragments being highly stable and soluble. Such binding members may be used in the treatment of inflammatory and other diseases as well as in diagnostics. Also provided are related nucleic acids, vectors, cells, and compositions.
Type:
Application
Filed:
November 5, 2013
Publication date:
May 15, 2014
Applicant:
Delenex Therapeutics AG
Inventors:
Stefanie Grabulovski, Titus Kretzschmar, Simone Schmitt, Abdijapar Shamshiev
Abstract: The invention features methods producing isoprene and a co-product, such as ethanol, 1,3-propanediol, or hydrogen from cultured cells. The invention also provides compositions that include these cultured cells. The invention provides compositions comprising isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen. Additionally, the invention provides methods of co-producing isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen by culturing cells under conditions suitable for co-production of isoprene and ethanol, isoprene and 1,3-propanediol, and isoprene and hydrogen.
Type:
Application
Filed:
January 6, 2014
Publication date:
May 8, 2014
Applicants:
The Goodyear Tire & Rubber Company, Danisco US Inc.
Inventors:
Gopal K. CHOTANI, Joseph C. MCAULIFFE, Caroline M. PERES, Karl J. SANFORD, Derek H. WELLS
Abstract: Three biosynthetic pathways are disclosed for microorganism bio-production of 1,4-Butanediol from various carbon sources. Exemplary methods are provided. The recombinant microorganisms comprising any of these 1,4-Butanediol biosynthesis pathways may also comprise genetic modifications directed to improved tolerance for 1,4-Butanediol.
Abstract: The present invention relates to an isolated polypeptide comprising (a) the amino acid sequence set forth in SEQ ID NO: 21; or (b) an amino acid sequence that is at least 95% identical to SEQ ID NO: 21, and to therapeutic treatments based thereon.
Abstract: The present disclosure relates to antigen-binding moieties that specifically bind to MIF and D-DT and compositions and methods of use thereof.
Abstract: The present invention provides compositions and methods for the expression of recombinant ?-glucosidase variants, as well as their use in the production of fermentable sugars from cellulosic biomass.
Type:
Application
Filed:
December 19, 2013
Publication date:
April 24, 2014
Applicant:
Codexis, Inc.
Inventors:
Dipnath Baidyaroy, Louis Clark, Lisa M. Newman
Abstract: The invention relates to methods for the screening, identification and/or application of microorganisms of use in imparting beneficial properties to plants. In one embodiment, the method involves subjecting one or more plants to a growth medium in the presence of a set of microorganisms, selecting one or more plant, acquiring one or more microorganisms associated with the selected plant(s) and repeating the process one or more times. The method further involves the step of subjecting the one or more microorganisms to a conditioning and/or directed evolution process.
Abstract: The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
December 13, 2013
Publication date:
April 24, 2014
Applicants:
Novozymes, Inc., Novozymes A/S
Inventors:
Lan Tang, Ye Liu, Junxin Duan, Yu Zhang, Christian Isak Jorgensen, Randall Kramer
Abstract: An acetyl xylan esterase variant having perhydrolytic activity is provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. More specifically, a Thermotoga maritima acetyl xylan esterase gene was modified using error-prone PCR and site-directed mutagenesis to create an enzyme catalyst characterized by an increase in specific activity. The variant acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
Type:
Application
Filed:
October 22, 2012
Publication date:
April 24, 2014
Applicant:
E. I. DU PONT DE NEMOURS AND COMPANY
Inventors:
ROBERT DICOSIMO, MARK SCOTT PAYNE, JOHN EDWARD GAVAGAN
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Type:
Application
Filed:
October 11, 2013
Publication date:
April 24, 2014
Inventors:
Suchindra Maiyuran, Randall Kramer, Paul Harris
Abstract: The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present invention relates to a method for constructing a host cell expressing a polypeptide of interest from at least two ORF's stably integrated onto the chromosome of the host cell comprising the steps of: a) providing the at least two ORF's encoding the same polypeptide, wherein the at least two DNA sequences of the ORF's differ in at least one position; b) integrating the at least two ORF's in the same orientation on the host cell chromosome.
Type:
Application
Filed:
September 23, 2013
Publication date:
April 17, 2014
Inventors:
Steen Troels Jorgensen, Poul Erik Pedersen
Abstract: Recombinant cells and methods are provided that relate to the use of isolated, engineered recombinant cells to directly or indirectly treat diseases or disorders in a mammalian host such as endocrine, gastrointestinal or autoimmune disorders. A recombinant cell is provided that comprises a signal sequence and a promoter, wherein: the signal sequence is capable of regulating signal-dependent expression of a target nucleic acid in a host or is capable of regulating signal-dependent expression of a target nucleic acid in response to an environmental stimulus, the cell is derived from an enteric or a commensal bacterium, and the target nucleic acid encodes a mammalian factor that promotes normal functioning of a physiological process in the host or is effective in preventing onset, establishment, or spread of a non-infectious disease in the host. The recombinant cell is administered to the host to treat the disease or disorder.
Abstract: IL-17 Receptor binding proteins, including non-naturally occurring and recombinantly modified proteins, methods of making and using such molecules as therapeutic, prophylactic and diagnostic agents are provided.
Abstract: The present invention relates to a method for production and purification of polypeptides. In particular, the present invention relates to a fusion protein comprising a solubility-enhancing peptide tag moiety, a self-aggregating peptide moiety and a moiety of target peptide and to a method for production and purification of target peptides through expressing said fusion protein.
Abstract: Genetically encoded calcium indicator (GECI) polypeptides and the nucleic acid molecules encoding such polypeptides are provided. In addition, methods of using such nucleic acids and polypeptides in methods of screening for agonists or antagonists of G-protein coupled receptor (GPCR) or ion channels and methods of monitoring neural activity also are provided.
Type:
Application
Filed:
March 13, 2013
Publication date:
April 10, 2014
Applicant:
HOWARD HUGHES MEDICAL INSTITUTE
Inventors:
Loren Looger, Karel Svoboda, Douglas Kim, Rex Kerr
Abstract: The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of Achromobacter xylosoxidans.
Type:
Grant
Filed:
May 24, 2012
Date of Patent:
April 8, 2014
Assignee:
Butamax Advanced Biofuels LLC
Inventors:
Michael G. Bramucci, Andrew C. Eliot, Lori Ann Maggio-Hall, Charles E. Nakamura
Abstract: Disclosed herein are isolated immunogens including variant gp120 polypeptides. In an example, a variant gp120 polypeptide includes a deletion of at least 8 consecutive residues of the fourth conserved loop (C4) between residues 419 and 434 of gp120 according to HXB2 numbering. Also provided are isolated nucleic acid molecules encoding the disclosed isolated immunogens. In an example, an isolated nucleic acid molecule further includes a nucleic acid molecule encoding a hepatitis B surface antigen or a variant thereof. Compositions including the isolated immunogens including variant gp120 polypeptides are also disclosed. In some examples, a composition further includes a carrier protein, such as a hepatitis B surface antigen or a variant thereof (natural or recombinant). Viral-like particles are also provided including any of the disclosed isolated immunogens or compositions.
Type:
Application
Filed:
November 25, 2013
Publication date:
April 3, 2014
Applicant:
The United States of America, as Represented by the Secretary, Dept. of Health & Human Service
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The present disclosure provides aromatic-cationic peptide compositions and methods of using the same. The methods comprise use of the peptides in electron transport and electrical conductance.
Type:
Application
Filed:
March 22, 2012
Publication date:
April 3, 2014
Applicants:
Stealth Peptides International, Inc., Cornell University
Inventors:
Hazel H. Szeto, Alexander V. Birk, D. Travis Wilson
Abstract: The invention relates to conjugates comprising a targeting moiety specific for the CXCR4 based on the polyphemusin-derived peptide and a therapeutic or imaging agent. The invention relates as well to the application of said conjugates for the therapy and diagnostics which require the specific targeting to CXCR4+ cells.
Type:
Application
Filed:
January 13, 2012
Publication date:
April 3, 2014
Inventors:
Antonio Pedro Villaverde Corrales, Esther Vázquez Gómez, Neus Ferrer Miralles, Ugutz Unzueta Elorza, Ramón Mangues Bafalluy, Maria Virtudes Céspedes Navarro, Isolda Casanova Rigat
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
Type:
Application
Filed:
October 12, 2012
Publication date:
April 3, 2014
Applicant:
Novozymes, Inc.
Inventors:
Kimberly Brown, Paul Harris, Elizabeth Zaretsky, Edward Re, Elena Vlasenko, Keith McFarland, Alfredo Lopez de Leon
Abstract: The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.
Type:
Grant
Filed:
February 3, 2010
Date of Patent:
April 1, 2014
Assignee:
LG Chem, Ltd.
Inventors:
Taek Ho Yang, Si Jae Park, Eun Jeong Lee, Hye Ok Kang, Tae Wan Kim, Sang Hyun Lee
Abstract: The invention provides for compositions and methods for producing isoprene by using recombinantly engineered cells that utilize a system of dual IspG enzymes in addition to isoprene synthase.
Type:
Grant
Filed:
December 22, 2011
Date of Patent:
April 1, 2014
Assignees:
Danisco US Inc., The Goodyear Tire & Rubber Company
Abstract: The invention relates to novel 7?-hydroxysteroid dehydrogenase mutants, to the sequences which encode these enzyme mutants, to processes for the preparation of the enzyme mutants and to their use in enzymatic reactions of cholic acid compounds, in particular in the preparation of ursodeoxycholic acid (UDCS). The invention also relates to novel processes for the synthesis of UDCS using the enzyme mutants; and to the preparation of UDCS using recombinant, multiply-modified microorganisms.
Type:
Application
Filed:
December 16, 2011
Publication date:
March 27, 2014
Applicant:
Pharmazell GMBH
Inventors:
Dirk Weuster-Botz, Michael Braun, Arno Aigner, Boqiao Sun, Christina Kantzow, Sven Bresch, Daniel Bakonyi, Werner Hummel
Abstract: In an aspect, the invention relates to compositions and methods production of n-butanol by aerobic hydrogen bacteria. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.
Type:
Application
Filed:
February 24, 2012
Publication date:
March 27, 2014
Applicant:
OHIO STATE INNOVATION FOUNDATION
Inventors:
F. Robert Tabita, Richard A. Laguna, Christopher J. Rocco, Sriram Satagopan, Andrew W. Dangel, Jon-David Swift Sears
Abstract: The invention provides fusion proteins comprising at least two fluorescent proteins, with the fluorescent proteins emitting different wavelengths of light from one another, at least one plant hormone binding domain that changes three-dimensional conformation upon specifically binding to a plant hormone, and two linker peptides, with the first linker linking the first fluorescent protein to the N-terminus of the plant hormone binding domain and the second linker linking the second fluorescent protein to the C-terminus of the plant hormone binding domain. The invention also provides for methods of using the fusion proteins of the present invention and nucleic acids encoding the fusion proteins.
Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
Type:
Application
Filed:
August 30, 2013
Publication date:
March 27, 2014
Applicant:
Danisco US Inc.
Inventors:
Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Abstract: The invention provides isolated amino acid sequences comprising the framework regions of an immunoglobulin heavy chain or light chain polypeptide, wherein certain amino acid residues of the framework regions are replaced with different amino acid residues that confer increased thermostability in vitro or in vivo. The invention also provides an isolated amino acid sequence of the constant region of an immunoglobulin heavy chain polypeptide wherein certain amino acid residues of the constant region are replaced with different amino acid residues that confer increased thermostability in vitro or in vivo.
Type:
Application
Filed:
September 19, 2013
Publication date:
March 20, 2014
Inventors:
Audrey MCCONNELL, David J. King, Peter M. Bowers