Abstract: The present invention provides host cells having an oxidizing cytoplasm, which are capable of growing to high cell densities and producing recombinant proteins in soluble form. Also provided are methods for growing such host cells to high densities, methods for optionally modulating the growth rates of the host cells, and methods for inducing the host cells to produce the desired gene product in soluble form. Further aspects of the invention relate to host cell preparation and storage methods, which utilize the advantages of producing gene products in host cell cytoplasm to provide methods for long-term storage and/or transport of expressed gene products that are retained in a stable and soluble form within the host cells.

Abstract: The present invention is directed to methods of producing algal biomass and algal cell cultures, and lipid compounds and compositions thereof, including fatty acids, carotenoids and fat soluble vitamins. The present invention is further directed to methods of preparing related food products and industrial and pharmaceutical compositions. In various exemplary embodiments, the methods comprise growing algae in a juice based medium, including a medium that contains natural nitrogen or a medium that is free of chemical additives and preservatives, to produce algal cell cultures, algal biomass, algae derived lipid compounds and compositions, and related products, all that can be certified organic.

Abstract: Methods and materials for treating bacterial vaginosis (“BV”) are provided. Cervicovaginal secretions (“CVS”) from a woman with vaginal microbiota dominated (>50%) by one of the species of lactobacillus typically found in the human vagina, e.g. Lactobacillus crispatus, L. iners, L. gasseri, L jensenii, is transplanted to women with BV as a method for restoring beneficial vaginal microbial communities and/or increasing resistance to sexually transmitted disease. Efficacy can be enhanced, or the properties of the endogenous CVS improved, through administration of an acidifying agent such as lactic acid. The examples demonstrate the role of healthy CVS in disease resistance, and the effect of pH on CVS properties. The examples also describe the collection and transplantation of healthy beneficial CVS into women at risk for, or after treatment for, BV.

Abstract: Provided are superabsorbent materials composed of agar, and one or more water-soluble natural polysaccharides, and dietary compositions containing such superabsorbent materials. The disclosed superabsorbent materials have various food and therapeutic applications and can be used as loading vehicles for nutrients and therapeutic agents. Also provided are methods for preparing such superabsorbent materials.

Abstract: The present disclosure discloses a preparation method for hyperthermophilic aerobic fermentation inoculant prepared by using sewage sludge, the method includes the following steps: carrying out fermentation after the activation of hyperthermophilic aerobic bacteria, removing the supernatant from the fermentation products, and adding the protective agent and stirring until uniform, drying to obtain a product, pulverizing the product by a pulverizer, and sieving the product before sub-packing. The solution of the present disclosure has the following advantages.

Abstract: An object of the present invention is to provide a novel composition for promoting the secretion of FGF21. The object is achieved by a composition for promoting the secretion of FGF21, including a Bifidobacterium bacterium as an active ingredient.

Abstract: Compositions and methods for increasing the efficacy of pesticidal compositions containing nicotinic acetylcholine receptor disrupters or allosteric modulators and C6-C10 saturated or unsaturated aliphatic acids are described herein, including synergistic pesticidal compositions and methods for delivery of pesticidal active ingredients. Some pesticidal compositions and methods as described are directed to compositions and methods for increasing the efficacy of fungicides. Some pesticidal compositions and methods as described are directed to compositions and methods for increasing the efficacy of nematicides. Some pesticidal compositions and methods as described are directed to compositions and methods for increasing the efficacy of insecticides. Methods for enhancing the activity pesticidal active ingredients in pesticidal compositions in use are also described.

Abstract: Disclosed herein is a method of utilizing an enzyme in a nucleic acid manipulation process, the method comprising: a) transforming a microorganism with a non-native enzyme; b) inducing expression of the enzyme in the microorganism, thereby producing the non-native enzyme; c) adding the microorganism of step b) directly to a non-naturally occurring nucleic acid manipulation process, wherein the non-native enzyme is not purified from the microorganism prior to addition to the nucleic acid manipulation process; and carrying out the nucleic acid manipulation process using the enzyme. Importantly, this method can be carried out without the need to purify the enzyme from the cell producing it before it is used in the nucleic acid manipulation method. Also disclosed herein is a kit for carrying out a nucleic acid manipulation process, the kit comprising a) a microorganism expressing a non-native enzyme; b) nucleic acids of interest; and c) reagents for use in the nucleic acid manipulation process.

Abstract: A process is provided for bioconversion of carbon monoxide and carbon dioxide. More specifically, the process includes fermenting carbon monoxide and carbon dioxide containing substrate with acetogenic bacteria. The process provides for high levels of carbon monoxide and carbon dioxide conversions and utilization of hydrogen.

Abstract: A CO2, bioconversion process includes providing a CO2 containing substrate to a bioreactor, the CO2 containing substrate including about 5 to about 90 mole % CO2; and fermenting the CO2 containing substrate with an acetogenic bacteria carrying a sodium translocating ATPase. The medium including less than about 0.01 grams per liter yeast extract, less than about 0.01 grams per liter carbohydrate, a sodium ion concentration provided by a sodium ion feed rate of about 290 to about 8750 ?g/gram of cells/minute, and a pH of about 4 to about 6.9.

Abstract: There is provided a method for treating silage which includes adding to the silage a silage inoculant including a silage preserving effective amount of Lactobacillus hilgardii. The silage inoculant being effective to prevent or reduce aerobic spoilage.

Abstract: A brain-penetrating composition of amyloid-ß binding peptide is disclosed. This may be useful in the treatment of Alzheimer's disease, for example as a bifunctional molecule, comprising a blood-brain barrier crossing antibody and an amyloid-ß targeting peptide linked via an Fc fragment that is able to transmigrate across the blood-brain barrier into the brain, and compositions comprising same. Methods of using this composition for treating Alzheimer's disease are disclosed.

Abstract: The application describes compositions that include maltosyl-isomalto-oligosaccharides with a desirable mass average molecular weight distribution. In some cases, the compositions can contain at least 3% mannitol.

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

Abstract: An isolated strain of Bifidobacterium longum NCIMB 42020 is useful for the treatment of viral infections, especially viral respiratory infections such as influenza, rhinovirus and RSV. Bifidobacterium longum NCIMB 42020 is also useful for clearing secondary bacterial infections.

Abstract: This disclosure provides intact bacterially derived minicells containing nucleic acids adjuvants or plasmids encoding nucleic acids adjuvants that can produce a desired immune response in target cells. This disclosure further provides methods that employ minicells to deliver nucleic acids adjuvants for use in the treatment of diseases, including neoplastic disease and cancer.

Abstract: Described herein are a solution, composition, and kit of poly (acetyl, arginyl) glucosamine (PAAG), methods of making the solution, and method of treating wounds with the solution, the method comprising administering to a subject an effective amount of a solution comprising PAAG, wherein the PAAG when administered topically contacts the wound, thereby treating the wound.

Abstract: The invention relates to a method for enriching protists with lipids rich in polyunsaturated fatty acids (PUFA) in particular of the omega 3 (?3) class, said method comprising the culture of protists in a culture medium comprising a selenium-containing compound as well as the protists enriched with lipids rich in PUFA obtained by this method. The invention also relates to a method for producing lipids rich in PUFA, comprising, after the enrichment method according to the invention, a method for treating the protists by extraction from the biomass of selenium-containing lipids rich in PUFA. The invention finally relates to any food, cosmetic or pharmaceutical product comprising either selenium-containing lipids thus extracted, or selenium-containing biomass originating from the enrichment method.

Abstract: A combination product comprising an isolated strain of Bifidobacterium NCIMB 41003 and another strain which does not adversely interact with Bifidobacterium NCIMB 41003 improves gastrointestinal symptoms associated with IBS and improves one or more of mood, stress, anxiety, sleep quality and depression associated with IBS.

Abstract: The present invention relates to a method including culturing a C1 compound-assimilating bacterium, which is a methylotroph, and/or a yeast by using a medium comprising, for example, a C1 compound and/or glycerol as a carbon source, to thereby produce EGT.

Abstract: The present invention is related to a novel plant defense signaling peptide and applications thereof.

Abstract: A method is described for recovering resources from a microbe supporting environment such as a water treatment system, comprising the steps of using microbial DNA sequencing to analyze the microbiome of the microbe supporting environment and identifying adjustments to the microbial content of the microbiome that will be useful in extracting resources from the microbe supporting environment such as a water treatment system, wherein the resources extracted can include, for example, methane released by microbes, nitrogen, phosphorus, or other contaminants generated by microbes, and/or clean water obtained by removing contaminants in a water treatment system.

Abstract: Provided herein is a microphysiological system that may be used in various cell culture applications and biological system studies, that facilitates co-culture, monitoring, and study of functional interactions among different types of cellular and biological materials under various environmental conditions. The system is configured for fast assembly and disassembly, thereby minimizing damage or contamination of the biological materials and environmental conditions inside the system. The system comprises a base, a lid, and a plurality of clamps that are connected to the base and the lid. The base has a recess surface for receiving cell culture support layers. The lid has a stepped surface configured to exert a contact force to a top layer of one or more cell culture support layers during use. The clamp involves an engagement mechanism that generates a contact force allowing for sealing of the cell culture support layers in a manner that minimizes leakage of fluids.

Abstract: The present disclosure relates to FR901464 and/or spliceostatin C compounds and their use as herbicides. Moreover, the invention relates to compositions for crop protection and to a method for controlling unwanted vegetation.

Abstract: The invention provides methods and compositions for treating fibromyalgia (FM) and chronic fatigue syndrome (CFS) in an individual. The methods provided herein entail administering a composition comprising an isolated Mycobacterium or antigenic fragments derived therefrom. Also provided herein are methods for assessing alleviation of symptoms and/or alteration of immune system functioning following administration of a composition comprising an isolated Mycobacterium or antigenic fragments derived therefrom.

Abstract: Nonnaturally occurring host cells altered to increase their ability to transfer genetic molecules into the host cells as compared to an unaltered host cell are provided. Also provided are methods for identifying endogenous loci of a host cell which inhibit transformation efficiency and/or electroporation of genetic molecules into the cell as well as methods for producing nonnaturally occurring host cells with enhanced transformation efficiency and/or the modified ability to allow for genomic integration of an exogenous DNA sequence via electroporation. Methods for producing biochemicals and products produced with the nonnaturally occurring host cells are also provided.

Abstract: The present invention provides a method for reducing plant water stress which comprises contacting plants with dry milled fibre, particularly wheat fibre and pea fibre. Said contacting is carried out in a manner selected from the group consisting of coating seeds with a mixture comprising dry milled fibre, pelletizing seeds with a mixture comprising dry milled fibre, applying a mixture comprising dry milled fibre in the form of granules, and applying a mixture comprising dry milled fibre in the form of tablets. Prior to said contacting, dry milled fibre is admixed with soil for plants, and the resulting mixture may optional comprise further additives such as fillers, binders, inert powders, colouring agents, fertilizers, fungicides, growth hormones, or microorganisms for inoculation.

Abstract: There is provided A process for producing a biocompatible soil mixture from cremated ash remains, the process comprising: mixing cremated ash remains with a soil base material and a nutrient composition to produce a pre-conditioned mixture, the nutrient composition comprising at least one of paramagnetic material, soil conditioner and fertilizer; and conditioning the pre-conditioned mixture using a microbial conditioner to adjust the adverse chemical properties of the cremated ash remains biologically to produce a conditioned soil mixture being biocompatible for a legacy tree, wherein the pre-conditioned mixture comprises less than 10% of the cremated ash remains by volume.

Abstract: Provided are modified Gram-negative bacteria having an increased periplasmic volume. Also provided are methods of expressing exogenous genes in the bacteria and targeting protein production to the periplasmic space.

Abstract: Decontaminating access structures that allow the partial insertion of decontamination tools into glove boxes and the like to easily and safely decontaminate harmful materials are provided.

Abstract: The present invention, in embodiments, is a composition and method of treatment using a composition that includes: a first component comprising: 75 to 97 weight percent of medium chain triglycerides; wherein the medium chain triglycerides comprise 6 to 12 carbon fatty acid esters of glycerol; 4 to 15 weight percent of soy lecithin; 0.05 to 1 weight percent of biotin; and 0.01 to 0.2 weight percent of antioxidant, and olive oil, wherein the antioxidant is selected from the group consisting of tocopherol, ascorbyl palmitate, Rosemary extract and mixtures thereof.

Abstract: The application relates to a computer-implemented method for creating a model of bioreaction—fermentation process or whole-cell catalysis process—with an organism on the basis of measured data.

Abstract: The purpose of the present invention is to provide a detection means whereby Staphylococcus aureus can be identified at a high accuracy. An aspect of the present invention relates to a medium for detecting S. aureus which comprises one or more kinds of nutrient components, a color developing agent capable of developing a color in the presence of ?-glucosidase, a color developing agent capable of developing a color in the presence of phosphatase and 0.5 mg/cm3 or more of sodium colistin methanesulfonate. Another aspect of the present invention relates to a sheet for detecting S. aureus, said sheet comprising the aforesaid medium, and a method for detecting S. aureus with the use of the medium and sheet as described above.

Abstract: Growth medium are disclosed for use in fermenting a marine microorganism. The medium comprise Potassium, Calcium, Strontium, Borate and Fluoride at specific concentrations. Alternatively, the growth medium comprises cobalt at specified concentrations or comprises vitamin B12 at specified concentrations. Methods of producing certain desired compound by fermentation of a marine microorganism are also disclosed.

Abstract: Compositions and methods are provided employing a helper strain system for promoting genetic alterations in a fungal host cell, e.g., a filamentous fungal host cell.

Abstract: Provided are a microorganism of the genus Escherichia for producing O-succinylhomoserine and a method for producing O-succinylhomoserine using the same.

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.

Abstract: The present invention relates to the topical use of Bifidobacterium longum subsp. longum CNCM I-2618, as a pro-pigmenting agent, in particular in the prevention and/or treatment of alterations of skin and/or hair color homogeneity and for creating a uniform complexion.

Abstract: Insecticidal toxins derived from Bacillus thuringiensis, polynucleotides encoding such toxins, use of such toxins to control Coleopteran plant pests, and transgenic plants that produce, and are protected, by these toxins are described.

Abstract: Provided herein are compositions and methods for the treatment or prevention of pathogenic infections.

Abstract: The present invention relates to beta-ketoacyl ACP synthase genes of the KASI/KASIV type and proteins encoded by these genes. The genes can be included in nucleic acid constructs, vectors or host cells. Expression of the gene products can alter the fatty acid profile of host cells. The KAS genes can be combined with a FATA or FATB thioesterase gene to create a cell that produces an increased amount of C8-C16 fatty acids. Suitable host cells include plastidic cells of plants or microalgae. Oleaginous microalga host cells with the new genes are disclosed.

Abstract: The present invention relates to a polypeptide having a resistant to feedback inhibition by methionine and an activity of homoserine O-succinyltransferase, a O-succinyl homoserine-producing microorganism expressing the polypeptide, and a method for producing O-succinyl homoserine using the same.

Abstract: The present disclosure is directed to methods of administering and compositions comprising Lactobacillus plantarum CECT 7478, Lactobacillus plantarum CECT 7485, and Pediococcus acidilactici CECT 7483 or a mutant or variant thereof. The compositions and methods are useful in the probiotic treatment of bowel diseases or conditions such as Inflammatory Bowel Disease, Irritable Bowel Syndrome, and abdominal distension and bloating.

Abstract: Probiotic Bifidobacterium strain AH1714 is significantly immunomodulatory following oral consumption. The strain is useful as an immunomodulatory biotherapeutic agent.

Abstract: The invention relates to a bacterial strain belonging to the genus Rhodococcus which is a producer of a nitrile hydratase. The invention also relates to a method for producing acrylamide by hydration of acrylonitrile using a biomass of the bacterial strain and to a method of culturing the bacterial strain.

Abstract: The subject invention pertains to methods for reducing the incidence or severity of citrus greening in desirable plants, such as orange trees, grapefruit trees, lime trees, lemon trees and the like.

Abstract: Provided is a separation and purification method for vancomycin hydrochloride of high purity. The method comprises the following steps: (1) obtaining a vancomycin hydrochloride solution from a crude vancomycin product by ion exchange chromatography and obtaining a concentrate by nanofiltration desalination and concentration; (2) adjusting the concentrate with a hydrochloric acid solution and then performing a column chromatography using a reverse chromatography column for the adjusted concentrate; (3) collecting the chromatographic solution of vancomycin to obtain a mixed chromatographic solution; (4) adjusting the mixed chromatographic solution, and separating the solution and the salts by nanofiltration desalination and concentration to obtain a concentrate; and (5) obtaining a vancomycin dry powder with a chromatographic purity of up to 99% and a pure white appearance by dehydrating and drying the concentrate of step (4), or by solvent crystallization or salting-out crystallization.

Abstract: An isolated promoter sequence comprising a nucleic acid of between 600 and 1700 nucleotides in length having at least 90% identity to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, or SEQ ID NO:7.

Abstract: A process is provided for fermentation of syngas that is effective for reducing conductivity and providing an alcohol STY of about 10 g ethanol/(L·day). The process includes introducing the syngas into a reactor vessel and providing a nitrogen feed rate to the reactor vessel of about 100 mg or more nitrogen/gram of cells produced. Fermentation of the syngas is effective for providing a fermentation medium having an average conductivity of about 16 mS/cm or less and an STY of 10 g ethanol/(L·day) or more.