Streptomyces Patents (Class 435/253.5)
  • Patent number: 10364446
    Abstract: The present invention relates to a bacterial strain, Streptomyces psammoticus OMK-4, and a method for vanillin production by fermentation of the bacterial strain. The method of the fermentation includes the following steps: activating the strain, culturing the seed, fermenting, extracting and so on. This method using ferulic acid as one of the raw materials to produce vanillin through microbial fermentation is safe and environmentally friendly.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: July 30, 2019
    Assignee: XIAMEN OAMIC BIOTECHNOLOGY CO., LTD.
    Inventors: Xijing Zhao, Chenguang Xing, Zhiqiang Huang, Wei Liu, Hongcan Gong
  • Patent number: 9856461
    Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an ?-olefin, such as 1-hexene or butadiene. The present invention also provides for a host cell comprising the PKS and when cultured produces the ?-olefin.
    Type: Grant
    Filed: September 14, 2015
    Date of Patent: January 2, 2018
    Assignee: The Regents of the University of California
    Inventors: Jeffrey L. Fortman, Leonard Katz, Eric J. Steen, Jay D Keasling
  • Patent number: 9040259
    Abstract: The present invention relates to production of polyketides and other natural products and to libraries of compounds and individual novel compounds. One important area is the isolation and potential use of novel FKBP-ligand analogs and host cells that produce these compounds. The invention is particularly concerned with methods for the efficient transformation of strains that produce FKBP analogs and recombinant cells in which cloned genes or gene cassettes are expressed to generate novel compounds such as polyketide (especially rapamycin) FKBP-ligand analogs, and to processes for their preparation, and to means employed therein (e.g. nucleic acids, vectors, gene cassettes and genetically modified strains).
    Type: Grant
    Filed: March 11, 2011
    Date of Patent: May 26, 2015
    Assignee: Buck Institute for Research on Aging
    Inventors: Matthew Alan Gregory, Sabine Gaisser, Hrvoje Petkovic, Steven Moss
  • Publication number: 20150133564
    Abstract: Provided are a novel compound, preparing method thereof, and use thereof as inhibitor of histone demethylase. The compound represented by Chemical Formula 1 has activity which inhibits histone demethylase and thus is capable of specifically and effectively inhibit activity of histone demethylase.
    Type: Application
    Filed: April 2, 2014
    Publication date: May 14, 2015
    Applicants: SNU R&DB FOUNDATION, INDUSTRY-ACADEMIC COOPERATION FOUNDATION, YONSEI UNIVERSITY, KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Dong-Chan OH, Seong-Hwan KIM, Jongheon SHIN, Hak Cheol KWON, So Hee KWON
  • Publication number: 20150030637
    Abstract: The invention relates to a method for fermenting soy flour in the solid state in order to reduce non-starch polysaccharides and alpha-galactosides, said method comprising the following steps: a) preparation of the fermentation substrate; b) inoculation of the substrate with selected celluloytic bacterial strains; c) incubation; and, optionally, d) drying of the product, which generates a product with: an increase in protein of between 12 and 15% compared to non-fermented soy flour, degradation of the alpha-galactosides of more than 90% compared to non-fermented soy flour, a reduction in non-starch polysaccharides (NSPs) of between 15 and 25%, an amino acid profile similar to that of non-fermented soy flour, and immune-stimulating effects.
    Type: Application
    Filed: January 29, 2013
    Publication date: January 29, 2015
    Applicant: Universidad de Chile
    Inventors: Jaime Moisés Romero Ormazábal, Rafael José Opazo Salas
  • Publication number: 20140295457
    Abstract: The present invention refers to the gene cluster and genes comprised by the gene cluster which are involved in the biosynthesis of griselimycin and methylgriselimycin and to the use of the gene cluster, genes comprised thereby and proteins encoded thereby for the production of antibiotic agents.
    Type: Application
    Filed: October 11, 2012
    Publication date: October 2, 2014
    Inventors: Mark Broenstrup, Claudia Koenig, Luigi Toti, Joachim Wink, Wulf Leuschner, Johann Gassenhuber, Rolf Müller, Silke Wenzel, Tina Binz, Carsten Volz
  • Patent number: 8815548
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: December 28, 2012
    Date of Patent: August 26, 2014
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Alex T. Nielsen, Dmitrii V. Vaviline
  • Publication number: 20140221205
    Abstract: Novel actinomycete strain composition and its use.
    Type: Application
    Filed: July 20, 2012
    Publication date: August 7, 2014
    Applicants: UNIVERSITE CADI AYYAD-FACULTE DES SCIENCES SEMLALIA, MARRAKESH-MAROC, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Marie-Joelle Virolle, Hanane Hamdali, Yedir Ouhdouch, Mohamed Hafidi, Ahmed Benhafrref, Ahmed Lebrihi, Marie Carmen Monje
  • Patent number: 8765441
    Abstract: The invention provides novel biologically pure cultures of microorganisms high in protease activity and capable of decomposing proteins recalcitrant to proteolysis as contained in garbage, waste water, organic waste liquids, industrial wastes and the like, a protease produced by such microorganisms and capable of decomposing proteins recalcitrant to proteolysis, and a method of utilizing the same. The novel culture is of a soil-derived microorganism belonging to Streptomyces sp., or a strain derived therefrom, which produces a protease capable of efficiently decomposing proteins recalcitrant to proteolysis as contained in waste water, organic waste liquids, industrial wastes and so forth.
    Type: Grant
    Filed: August 9, 2011
    Date of Patent: July 1, 2014
    Assignee: Microbial Chemistry Research Foundation
    Inventors: Hiroyasu Doi, Naoko Kinoshita, Tatsuzo Oka, Zhao Hui
  • Patent number: 8765404
    Abstract: Some aspects of this invention relate to methods useful for the conversion of a carbon source to a biofuel or biofuel precursor using engineered microbes. Some aspects of this invention relate to the discovery of a key regulator of lipid metabolism in microbes. Some aspects of this invention relate to engineered microbes for biofuel or biofuel precursor production.
    Type: Grant
    Filed: March 2, 2011
    Date of Patent: July 1, 2014
    Assignee: Massachusetts Institute of Technology
    Inventors: Gregory Stephanopoulos, Syed Hussain Imam Abidi
  • Publication number: 20140105862
    Abstract: The present invention relates to novel strains of Streptomyces microflavus and methods of their use for controlling diseases or pests of a plant. The invention also relates to a fermentation broth obtained by cultivating a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin. The invention also relates to a method of producing a fermentation broth of a gougerotin producing Streptomyces strain, wherein the fermentation broth contains at least about 1 g/L gougerotin, the method comprising cultivating the Streptomyces strain in a culture medium containing a digestible carbon source and a digestible nitrogen source under aerobic conditions, wherein the culture medium contains an amino acid at a concentration effective to achieve a gougerotin concentration of at least 1 g/L.
    Type: Application
    Filed: October 11, 2013
    Publication date: April 17, 2014
    Applicant: Bayer CropScience LP
    Inventors: Brian CAMPBELL, Damian CURTIS, Shaohua GUAN, Magalie GUILHABERT-GOYA, Daniel M. JOO, Tara LU, Jonathan S. MARGOLIS, Reed Nathan ROYALTY, Gerardo Bueno SALAZAR, David SESIN, Frisby Davis SMITH, Colleen TAYLOR, Hong ZHU
  • Publication number: 20140109249
    Abstract: The present invention relates to methods for the screening, identification and/or application of microorganisms of use in imparting beneficial properties to plants.
    Type: Application
    Filed: October 10, 2013
    Publication date: April 17, 2014
    Applicant: Biodiscovery New Zealand Limited
    Inventors: Susan Jane Turner, Peter John Wigley
  • Patent number: 8697403
    Abstract: Disclosed are processes for producing lipids for biofuel or lubricant and Streptomyces bacteria used in the processes. The processes include steps whereby bacterial cells of the germs Streptomyces are cultivated in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), wherein the organic waste(s) or residue(s) comprise lignocellulosic waste(s) or lignocellulosic residue(s), recovering lipids from the cells of the bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: April 15, 2014
    Assignee: Neste Oil Oyj
    Inventors: Maria Holmback, Miina Lehesto, Perttu Koskinen, Johan-Fredrik Selin
  • Publication number: 20140087442
    Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: August 30, 2013
    Publication date: March 27, 2014
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Patent number: 8664186
    Abstract: The present invention relates to Stambomycin compounds, their derivatives and their pharmaceutically acceptable salt thereof.
    Type: Grant
    Filed: July 23, 2010
    Date of Patent: March 4, 2014
    Assignees: Universite de Lorraine, University of Warwick, Institut National de la Recherche Agronomique
    Inventors: Bertrand Aigle, Gregory Challis, Luisa Laureti, Lijiang Song, Pierre Leblond
  • Publication number: 20140051152
    Abstract: There are provided novel A-87774 compounds or salts thereof having a herbicidal activity or a plant growth regulating activity, a microorganism producing the same, a production method thereof, an agrochemical (in particular, herbicide or plant growth regulator) containing the same as an active ingredient, a method for using the same, and a culture of the microorganism.
    Type: Application
    Filed: October 15, 2013
    Publication date: February 20, 2014
    Applicant: MITSUI CHEMICALS AGRO, INC.
    Inventors: Yoshio Shigematsu, Yoshiko Sugie, Masaaki Kizuka
  • Publication number: 20140030242
    Abstract: Disclosed are a novel strain with antibacterial activity, identified as Streptomyces sp. AN1542 strain (Accession No.: KCTC 12113BP), an antibacterial composition comprising a compound represented by Chemical Formula 1 or 2, or a strain producing the same as an active ingredient, and a method for producing the same. Exhibiting potent antibacterial activity against pathogenic microbes and antibiotic-resistant microbes, particularly, MRSA, QRSA, VRSA and VRE, the compound or the strain of the present invention can be applied to the treatment of infectious diseases caused by superbacteria.
    Type: Application
    Filed: January 4, 2012
    Publication date: January 30, 2014
    Inventors: Won Gon Kim, Yun Ju Kwon, Mi Jin Sohn
  • Patent number: 8603799
    Abstract: Methods of controlling plant diseases mediated by bacterial or fungal plant pathogens. The method comprises providing Streptomyces scopuliridis strain RB72 or an isolated protein or polypeptide comprising the amino acid of SEQ ID NO:1 and applying the Streptomyces scopuliridis strain RB72 or the isolated protein or polypeptide comprising the amino acid of SEQ ID NO:1 to plants or plant seeds under conditions effective to treat plant diseases mediated by bacterial or fungal plant pathogens. Also disclosed is a plant or plant seed treated by this method, a planting composition, and a method of enhancing growth.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: December 10, 2013
    Assignee: Bioworks, Inc.
    Inventors: William R. Martin, Jr., Nicole J. Scott
  • Publication number: 20130316408
    Abstract: The invention discloses marine Streptomyces sp., a Pyranosesquiterpene compound, as well as a preparation method and uses thereof. Streptomyces sp. SCSIO 01689 was collected in China Center for Type Culture Collection (CCTCC) (Address: Wuhan University, Wuhan City, China) with the collection number of CCTCC NO: M 2011257 on Jul. 18, 2011. The Streptomyces sp. SCSIO 01689 can produce the Pyranosesquiterpene compound with better anti-Escherichia coli activity and anti-Artemia activity, as well as Cyclo(D)-Pro-(D)-Ile, Cyclo(D)-Pro-(D)-Leu and Cyclo(D)-trans-4-OH-Pro-(D)-Phe with better anti-Vibrio anguillarum activity and anti-Artemia activity, thereby providing a new way for preparing the Cyclo(D)-Pro-(D)-Ile, the Cyclo(D)-Pro-(D)-Leu and the Cyclo(D)-trans-4-OH-Pro-(D)-Phe. The Pyranosesquiterpene compound can be used for preparing anti-Escherichia coli medicaments and anti-Artemia medicaments, as well as preparing condiments as a condiment precursor compound.
    Type: Application
    Filed: June 18, 2012
    Publication date: November 28, 2013
    Applicant: South China Sea Institute Of Oceanology, Chinese Academy of Sciences
    Inventors: Lijuan Long, Xinpeng Tian, Jie Li, Xiongming Luo, Zhenxiong Qi, Tuan Yin
  • Publication number: 20130288340
    Abstract: An object of the present invention is to provide a microorganism which is novel at the elemental composition level, and to provide a technique for providing such a microorganism. The present inventor has succeeded in causing a microorganism to efficiently contain a non-essential element by decreasing the content of an essential nutrient source for the microorganism, a C source, an N source, a P source, or an S source, and by adding an X compound containing the non-essential element as a constitutive element in a manner to make up for the decreased amount, and then culturing the microorganism.
    Type: Application
    Filed: September 5, 2011
    Publication date: October 31, 2013
    Applicants: PARA MICROBIOS LABORATORIES LLC
    Inventor: Yoshitake Tanaka
  • Patent number: 8563266
    Abstract: There are provided novel A-87774 compounds or salts thereof having a herbicidal activity or a plant growth regulating activity, a microorganism producing the same, a production method thereof, an agrochemical (in particular, herbicide or plant growth regulator) containing the same as an active ingredient, a method for using the same, and a culture of the microorganism.
    Type: Grant
    Filed: August 13, 2010
    Date of Patent: October 22, 2013
    Assignee: Mitsui Chemicals Agro, Inc.
    Inventors: Yoshio Shigematsu, Yoshiko Sugie, Masaaki Kizuka
  • Publication number: 20130237490
    Abstract: The present invention relates to novel therapeutics with antibacterial activity, processes for their preparation, and pharmaceutical, veterinary and nutritional compositions containing them as active ingredients. The present invention also relates to uses of the novel therapeutics, for example, as medicants or food additives in the treatment of bacterial infections or to aid body mass gain in a subject.
    Type: Application
    Filed: September 17, 2010
    Publication date: September 12, 2013
    Applicant: Albany Molecular Research, Inc.
    Inventors: Grant J. CARR, David D. MANNING, Zhicai YANG, Cheng GUO, Jun-Ho MAENG, John RABENSTEIN, Peter C. MICHELS, Matthew W. CHASE
  • Publication number: 20130211100
    Abstract: The present invention describes a method to generate new chemical entities (NCEs) that have well-defined activities such as, but not limited to, anti-bacterial, antifungal and anthelmintic effects. The NCEs are generated through adaptive evolution of one microbe (the producer) against another organism or cell type (the target). The producer is made to compete against the target over time by co-culturing the two together and serially passing the producer organism until the producer adaptively evolves by synthesizing an NCE(s) that inhibits growth of or kills the target. The molecular structure of the chemical entity (or entities) is then clucidated using tools from genomics, molecular biology, computational biology, analytical chemistry, organic chemistry and related fields.
    Type: Application
    Filed: October 28, 2011
    Publication date: August 15, 2013
    Inventors: Bernhard Palsson, Pep Charusanti
  • Publication number: 20130189764
    Abstract: Disclosed is a method of selectively inhibiting growth of non-target cells in a mixed population of target and non-target cells, the method comprising the steps of: (a) contacting the mixed population with a selective agent which comprises a carrier moiety linked by a scissile linkage to a toxic moiety; wherein the selective agent is able to enter non-target cells in which the scissile linkage is cleaved, releasing the toxic moiety to exert a toxic effect on the non-target cells causing inhibition of the growth of the non-target cells, whereas the selective agent is unable to enter target cells and/or the scissile linkage is not cleaved in target cells and/or toxic moiety, if released from the selective agent, does not exert a toxic effect on the target cell; and (b) culturing the cells in conditions which allow for growth of non-inhibited cells.
    Type: Application
    Filed: March 11, 2013
    Publication date: July 25, 2013
    Applicant: Oxide Limited
    Inventor: Oxide Limited
  • Publication number: 20130189760
    Abstract: The purpose of the present invention is to provide a nuclease that secretes natural nonpathogenic microorganisms extracellularly, has higher specific activity than conventional nucleases, and is useful in nucleolytic degradation on an industrial scale. This purpose is achieved with an extracellularly secreted nuclease derived from Streptomyces bacteria, the nuclease having specific activity equal to or greater than the specific activity of Benzonase® when supplied to double-stranded DNA for 30 minutes at 37° C. in 20 mM Tris/HCl (pH 8.5) containing 1 mM MgCl2 and 1 mM CaCl2 after purification, using double-stranded DNA, single-stranded DNA, and RNA as substrates.
    Type: Application
    Filed: September 15, 2011
    Publication date: July 25, 2013
    Applicant: Independent Administrative Institution Japan Agency For Marine-Earth Science
    Inventors: Kozue Mori, Yukari Ohta, Yuji Hatada, Nobuyuki Nakamura, Masayuki Miyazaki
  • Patent number: 8431369
    Abstract: A method for the preparation of the polyunsaturated fatty acids-containing phosphatidylserine, the method comprising: combining L-serine with a fish liver phosphatidylcholine having a polyunsaturated fatty acid to form a mixture; reacting the mixture with phospholipase D to effect transphosphatidylation of L-serine and the phosphatidylcholine having polyunsaturated fatty acids to produce the polyunsaturated fatty acids-containing phosphatidylserine.
    Type: Grant
    Filed: January 27, 2011
    Date of Patent: April 30, 2013
    Inventors: Su Chen, Hung Kwong
  • Patent number: 8361762
    Abstract: The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene.
    Type: Grant
    Filed: September 15, 2009
    Date of Patent: January 29, 2013
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Anthony R. Calabria, Michael C. Miller, Dmitrii V. Vaviline, Alex T. Nielsen
  • Patent number: 8344119
    Abstract: A process for the production of natural ferulic acid, coniferyl alcohol and/or vanillin, includes the bio-conversion of eugenol by a bacteria belonging to the Streptomyces genes including at least one nucleotide sequence SEQ ID NO:1 or SEQ ID NO:8 or any nucleotide sequence having at least 70%, preferably 80% and very preferably 90%, identity with the sequence SEQ ID NO:1 or SEQ ID NO:8.
    Type: Grant
    Filed: February 20, 2008
    Date of Patent: January 1, 2013
    Assignee: V. Mane Fils
    Inventors: Fanny Lambert, Joseph Zucca, Jean Mane
  • Patent number: 8247211
    Abstract: The invention provides compounds of formula Wherein R1, R2, R3, R4, R5, R6a, R6b, R6c, R6d, R6e and R7 are defined in the specification. These compounds are useful as antibiotic agents.
    Type: Grant
    Filed: February 8, 2011
    Date of Patent: August 21, 2012
    Assignees: American Cyanamid Company, Wyeth Holdings Corporation
    Inventors: Jason Arnold Lotvin, Mark Edward Ruppen
  • Publication number: 20120202273
    Abstract: This invention relates to enzymatic removal of type A and B antigens from blood group A, B, and AB reactive cells in blood products, and thereby converting these to non-A and non-B reactive cells. The invention further relates to using unique ?-N-acetylgalactosaminidases and ?-galactosidases with superior kinetic properties for removing the immunodominant monosaccharides of the blood group A and B antigens and improved performance in enzymatic conversion of red blood cells. The preferred unique ?-N-acetylgalactosaminidases and ?-galactosidases exhibit the following characteristics: (i) exclusive, preferred or no less than 10% substrate specificity for the type A and B branched polysaccharide structures relative to measurable activity with simple mono- and disaccharide structures and aglycon derivatives hereof; (ii) optimal performance at neutral pH with blood group oligosaccharides and in enzymatic conversion of cells; and (iii) a favorable kinetic constant Km with mono- and oligosaccharide substrates.
    Type: Application
    Filed: August 8, 2011
    Publication date: August 9, 2012
    Inventors: Henrik Clausen, Humberto de la Vega, Chery Hill, Qiyong Peter Liu
  • Patent number: 8206966
    Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.
    Type: Grant
    Filed: November 3, 2008
    Date of Patent: June 26, 2012
    Assignee: Danisco US Inc.
    Inventors: Luis G. Cascāo-Pereira, James T. Kellis, Jr., Bradley A. Paulson, Scott D. Power, Sandra W. Ramer, Vivek Sharma, Andrew Shaw, Jayarama K. Shetty, Donald E. Ward
  • Publication number: 20120149575
    Abstract: There are provided novel A-87774 compounds or salts thereof having a herbicidal activity or a plant growth regulating activity, a microorganism producing the same, a production method thereof, an agrochemical (in particular, herbicide or plant growth regulator) containing the same as an active ingredient, a method for using the same, and a culture of the microorganism.
    Type: Application
    Filed: August 13, 2010
    Publication date: June 14, 2012
    Applicant: MITSUI CHEMICALS AGRO, INC.
    Inventors: Yoshio Shigematsu, Yoshiko Sugie, Masaaki Kizuka
  • Publication number: 20120124898
    Abstract: This invention provides co-cultures of photosynthetic microorganisms and biofuel producing microorganisms. In certain embodiments, polysaccharide-producing, photosynthetic microorganisms are microalgae having frustules provide a substrate on which biofuel-producing microorganisms can grow. In other embodiments, the photosynthetic microorganisms produce a lipid and the non-photosynthetic microorganisms produce a solvent in which the lipid is soluble.
    Type: Application
    Filed: July 21, 2011
    Publication date: May 24, 2012
    Inventor: Pamela R. Contag
  • Publication number: 20120028799
    Abstract: Methods of controlling plant diseases mediated by bacterial or fungal plant pathogens. The method comprises providing Streptomyces scopuliridis strain RB72 or an isolated protein or polypeptide comprising the amino acid of SEQ ID NO:1 and applying the Streptomyces scopuliridis strain RB72 or the isolated protein or polypeptide comprising the amino acid of SEQ ID NO:1 to plants or plant seeds under conditions effective to treat plant diseases mediated by bacterial or fungal plant pathogens. Also disclosed is a plant or plant seed treated by this method, a planting composition, and a method of enhancing growth.
    Type: Application
    Filed: July 29, 2011
    Publication date: February 2, 2012
    Applicant: BIOWORKS, INC.
    Inventors: William R. MARTIN, JR., Nicole J. SCOTT
  • Publication number: 20120010163
    Abstract: The present invention relates to novel therapeutics with antibacterial activity, processes for their preparation, and pharmaceutical, veterinary and nutritional compositions containing them as active ingredients. The present invention also relates to uses of the novel therapeutics, for example, as medicants or food additives in the treatment of bacterial infections or to aid body mass gain in a subject.
    Type: Application
    Filed: September 17, 2010
    Publication date: January 12, 2012
    Applicant: Albany Molecular Research, Inc.
    Inventors: GRANT J. CARR, DAVID D. MANNING, ZHICAL YANG, CHENG GUO, JUN-HO MAENG, JOHN RABENSTEIN, PETER C. MICHELS, MATTHEW W. CHASE
  • Publication number: 20120003740
    Abstract: This document discloses electroporation vessels, electrocompetent cells that have been aliquoted and frozen in electroporation vessels, and a number of other apparatuses, kits, and methods for electroporation. Some embodiments of electroporation vessels described herein may include a pair of opposing walls that are downwardly angled toward one another in a gap between two electrode surfaces. Further embodiments of devices and methods described herein may eliminate the need for an end user to transfer competent cells from a capped tube to electroporation cuvette, thereby saving time and producing less waste.
    Type: Application
    Filed: December 28, 2010
    Publication date: January 5, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Laura Vozza-Brown, Harry Yim, John Cameron, Matthew O'Banion, Adam Scott Henry, Lisa Marie Olivier
  • Publication number: 20110294173
    Abstract: The present invention provides a process for producing lipids for biofuel or lubricant and Streptomyces bacteria used in the process. The process comprises cultivating bacterial cells of the genus Streptomyces in a medium comprising organic waste(s) or residue(s) or mixtures thereof as carbon and/or nutrient source(s), recovering lipids from the cells of said bacteria or from the cultivation medium, and using the recovered lipids or a fraction thereof as biofuel and/or lubricant, or as a starting material for biofuel and/or lubricant production. In addition, in one aspect the invention provides products obtained by using the process according to this disclosure.
    Type: Application
    Filed: May 24, 2011
    Publication date: December 1, 2011
    Applicant: NESTE OIL OYJ
    Inventors: Maria Holmback, Miina Lehesto, Perttu Koskinen, Johan-Fredrik Selin
  • Publication number: 20110295009
    Abstract: The invention relates to streptospirole derivatives of the general formula (I), wherein R1, R2, R3, X1, X2, Y1 and Y2 are as defined herein, a process for the preparation of said compounds by fermenting the microorganism Streptomyces sp. ST 108140 (DSM 19369) and optionally derivatizing the compounds produced by said microorganism, a pharmaceutical composition comprising at least one compound of the formula (I), and the use of a compound of the formula (I) for the preparation of a medicament for the treatment and/or prophylaxis of bacterial infections.
    Type: Application
    Filed: July 16, 2009
    Publication date: December 1, 2011
    Applicant: SANOFI-AVENTIS
    Inventors: Cosima Dufour-Schroif, Joachim Wink, Martin Gerlitz, Helene Olivan, Michael Kurz
  • Patent number: 8058026
    Abstract: The invention provides novel biologically pure cultures of microorganisms high in protease activity and capable of decomposing proteins recalcitrant to proteolysis as contained in garbage, waste water, organic waste liquids, industrial wastes and the like, a protease produced by such microorganisms and capable of decomposing proteins recalcitrant to proteolysis, and a method of utilizing the same. The novel culture is of a soil-derived microorganism belonging to Streptomyces sp., or a strain derived therefrom, which produces a protease capable of efficiently decomposing proteins recalcitrant to proteolysis as contained in waste water, organic waste liquids, industrial wastes and so forth.
    Type: Grant
    Filed: January 18, 2008
    Date of Patent: November 15, 2011
    Assignee: Microbial Chemistry Research Foundation
    Inventors: Hiroyasu Doi, Naoko Kinoshita, Tatsuko Oka, Zhao Hui
  • Publication number: 20110151546
    Abstract: The invention provides compounds of formula Wherein R1, R2, R3, R4, R5, R6a, R6b, R6c, R6d, R6e and R7 are defined in the specification. These compounds are useful as antibiotic agents.
    Type: Application
    Filed: February 8, 2011
    Publication date: June 23, 2011
    Applicants: AMERICAN CYANAMID COMPANY, Wyeth Holdings Corporation
    Inventors: Jason Arnold Lotvin, Mark Edward Ruppen
  • Publication number: 20110144355
    Abstract: A compound having a structure expressed by the following Structural Formula (1) and a compound having a structure expressed by the following Structural Formula (2):
    Type: Application
    Filed: February 23, 2011
    Publication date: June 16, 2011
    Applicant: KINKI UNIVERSITY
    Inventors: Masayuki Igarashi, Ryutaro Utsumi
  • Publication number: 20110143394
    Abstract: The invention relates to the field of biochemistry, molecular biology and microbiology. More specific the invention relates to methods and means for metabolic engineering and improved product formation by a filamentous micro-organism or a low G+C gram-positive bacterium. The invention discloses that DasR and DasR binding sites play an important and universal role in the control of gene expression in micro-organisms Based on this finding, the invention provides multiple useful applications, such as a method for regulating the expression of a gene of interest, a method for controlling metabolism, a method for decreasing undesired expression and many more. Moreover, the invention also provides means that can be used to establish said methods: for example a micro-organism in which the DasR binding site in operable linkage with a particular gene has been modified to obtain increased or decreased expression of a protein (being a desired or undesired protein) encoded by said gene.
    Type: Application
    Filed: February 14, 2007
    Publication date: June 16, 2011
    Applicants: Universiteit Leiden, University of Liege, Friedrich-Alexander-Universitat Erlangen-Nurnberg
    Inventors: Sebastien Rigali, Mannfred F.B. Titgemeyer, Gilles P. van Wezel
  • Publication number: 20110144176
    Abstract: This invention relates, in part, to macrolide compounds, actinomycete strains for producing them, and pharmaceutical compositions containing them.
    Type: Application
    Filed: December 15, 2010
    Publication date: June 16, 2011
    Applicant: Wyeth LLC
    Inventors: Mia Haruna Yoshino SUMMERS, Edmund Idris Graziani, Margaret M. Leighton, Kevin Pong, Roger Alan Kele, David P. Labeda
  • Publication number: 20110143940
    Abstract: A new Streptomyces beta-vulgaris strain that is deposited at the CNCM under number I-3639, and the use of this strain or a mutant strain for obtaining a composition (culture filtrate) and active compounds (secondary metabolites) by fermentation. Also, the use of the new strain, a mutant strain, the culture filtrate, or active compounds for the protection of plants and the biological struggle against phytopathogenic agents and/or for stimulating the growth of plants.
    Type: Application
    Filed: June 19, 2009
    Publication date: June 16, 2011
    Applicants: AGRONUTRITION, INSTITUT NATIONAL POLYTECHNIQUE DE TOULOUSE
    Inventors: Ahmed Lebrihi, Rafik Errakhi, Mustapha Barakate
  • Publication number: 20110117606
    Abstract: The present invention provides a nucleic acid molecule comprising: (a) a nucleotide sequence as shown in SEQ ID No. 1; or (b) a nucleotide sequence which is the complement of SEQ ID No. 1; or (c) a nucleotide sequence which is degenerate with SEQ ID No. 1; or (d) a nucleotide sequence having at least 85% sequence identity (preferably at least 87%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity) with SEQ ID No. 1; or (e) a part of any one of (a) to (d), wherein said nucleic acid molecule encodes or is a complementary to a nucleic acid molecule encoding one or more polypeptides, or comprises or is complementary to a nucleic acid molecule comprising one or more genetic elements, having functional activity in the synthesis of a polyketide-based or macrolactam molecule.
    Type: Application
    Filed: March 20, 2009
    Publication date: May 19, 2011
    Inventors: Hanne Jørgensen, Trond Erling Ellingsen, Kristin Fløgstad Degnes, Per Bruheim, Håvard Sletta, Espen Fjaervik, Geir Klinkenberg, Sergey Zotchev
  • Publication number: 20110105376
    Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amy-lases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.
    Type: Application
    Filed: March 20, 2008
    Publication date: May 5, 2011
    Inventors: George England, Marc Kolkman, Casper Vroemen
  • Publication number: 20110105420
    Abstract: [Object] Disclosed is a compound useful as an active ingredient for a pharmaceutical composition having an ?-amylase inhibitory activity, particularly a pharmaceutical composition for the treatment of diabetes. [Solution] An investigation has been made on a compound having an ?-amylase inhibitory activity among the compounds produced by Streptomyces sp. strain 6982, which is a actinomycetes belonging to the genus Streptomyces, and it was confirmed that an aminosugar compound has an ?-amylase inhibitory activity, thus completing the invention. The aminosugar compound of the present invention has an ?-amylase-inhibitory activity, and therefore can be used as a prophylactic or therapeutic agent for diabetes, obesity, NASH (non-alcoholic steatohepatitis), particularly as an ameliorating agent for postprandial blood glucose elevation.
    Type: Application
    Filed: April 9, 2009
    Publication date: May 5, 2011
    Applicant: Astellas Pharma Inc.
    Inventors: Satoshi Sasamura, Hiromi Sasamura, Shinya Nishiwaki
  • Patent number: 7935360
    Abstract: The invention concerns the application of compositions of micro-organisms in biological control of vine cryptogamic diseases. Said composition comprises a mixture of at least one bacterium and at least one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant. The invention also concerns bacterial and yeast strains, as well as biofungicide formulations containing an efficient amount of at least one composition of micro-organisms including in mixture at least one bacterium and one yeast, the bacterium or bacteria and the yeast(s) being non-toxic for the plant, and a composition of filamentous fungi, in particular of the genus Pichia, Pythium, Trichoderma, Gliocladium, Ampelomyces, Talaromyces, Epicococcum, combined with an inert carrier. The invention is useful for treating cryptogamic plant diseases, in particular crop plants and vine.
    Type: Grant
    Filed: July 1, 2003
    Date of Patent: May 3, 2011
    Assignee: Universite de Bourgogne
    Inventor: Bernard Paul
  • Publication number: 20110045569
    Abstract: This invention relates to enzymatic removal of type A and B antigens from blood group A, B, and AB reactive cells in blood products, and thereby converting these to non-A and non-B reactive cells. The invention further relates to using unique ?-N-acetylgalactosaminidases and ?-galactosidases with superior kinetic properties for removing the immunodominant monosaccharides of the blood group A and B antigens and improved performance in enzymatic conversion of red blood cells. The preferred unique ?-N-acetylgalactosaminidases and ?-galactosidases exhibit the following characteristics: (i) exclusive, preferred or no less than 10% substrate specificity for the type A and B branched polysaccharide structures relative to measurable activity with simple mono- and disaccharide structures and aglycon derivatives hereof; (ii) optimal performance at neutral pH with blood group oligosaccharides and in enzymatic conversion of cells; and (iii) a favorable kinetic constant Km with mono- and oligosaccharide substrates.
    Type: Application
    Filed: May 28, 2010
    Publication date: February 24, 2011
    Inventors: Henrik Clausen, Humberto De La Vega, Cheryl Hill, Qiyong Peter Liu
  • Publication number: 20110015248
    Abstract: The present invention relates to substituted heterocycles, processes for their preparation, and their use in medicaments, especially for the treatment of inflammatory disease, i.e. asthma, or cancer.
    Type: Application
    Filed: January 8, 2009
    Publication date: January 20, 2011
    Applicant: INTERMED DISCOVERY GMBH
    Inventors: Marc STADLER, Stephan Seip, Hartwig Müller, Anke Mayer-Bartschmid, Michael-Alexander Brüning, Jordi Benet-Buchholz, Hiroko Togame, Reiko Dodo, Peter Reinemer, Kevin Bacon, Kinji Fuchikami, Satoko Matsukawa, Klaus Urbahns