Transformants Patents (Class 435/257.2)
  • Micoorganisms and Methods for the Production of Fatty Acids and Fatty Acid Derived Products
    Publication number: 20140051136
    Abstract: This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a fatty acid or fatty acid derived product, wherein the modified microorganism produces fatty acyl-CoA intermediates via a malonyl-CoA dependent but malonyl-ACP independent mechanism
    Type: Application
    Filed: August 9, 2013
    Publication date: February 20, 2014
    Applicant: OPX Biotechnologies, Inc.
    Inventors: Hans Liao, Eileen Spindler, Joseph R. Warner, Michael Louie, Wendy Ribble, Brittany Prather, Ron Evans, Tanya E.W. Lipscomb, Michael D. Lynch
  • Platensimycin biosynthetic gene cluster of
    Patent number: 8652838
    Abstract: The present invention relates to the cloning and sequence of a biosynthetic gene cluster from Streptomyces platensis that produces platensimycin and platencin. Also provided are engineered micro-organisms for the production of these compounds, and analogs thereto, as well as methods of screening for compounds with anti-bacterial activity.
    Type: Grant
    Filed: July 24, 2008
    Date of Patent: February 18, 2014
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Ben Shen, Michael J. Smanski
  • Methods and Compositions for Producing Alkenes of Various Chain Length
    Publication number: 20140038255
    Abstract: The NonA alkene synthase in Synechococcus sp. displays selective synthesis of 1-nonadecene. Heterologous recombination of a domain, i.e. the acyl binding domain, with other acyl binding proteins, affects acyl substrate chain-length binding selectivity and therefore the chain-length of the synthesized 1-alkenes. Compositions and methods are provided to selectively synthesize 1-alkenes of various chain lengths.
    Type: Application
    Filed: October 7, 2013
    Publication date: February 6, 2014
    Applicant: JOULE UNLIMITED TECHNOLOGIES, INC.
    Inventor: Christian Perry Ridley
  • ANTI-EPIDERMAL GROWTH FACTOR RECEPTOR VARIANT III CHIMERIC ANTIGEN RECEPTORS AND USE OF SAME FOR THE TREATMENT OF CANCER
    Publication number: 20140037628
    Abstract: The disclosure provides chimeric antigen receptors (CARs) comprising an antigen binding domain of human antibody 139, an extracellular hinge domain, a transmembrane domain, and an intracellular domain T cell receptor signaling domain. Nucleic acids, recombinant expression vectors, host cells, populations of cells, antibodies, or antigen binding portions thereof, and pharmaceutical compositions relating to the CARs are disclosed. Methods of detecting the presence of cancer in a host and methods of treating or preventing cancer in a host are also disclosed.
    Type: Application
    Filed: March 21, 2012
    Publication date: February 6, 2014
    Applicant: The United States of America, as rep., by the Secretary, Dept., of Health and Human Services.
    Inventors: Richard A. Morgan, Steven A. Rosenberg
  • MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY
    Publication number: 20140024089
    Abstract: The invention relates to recombinant expression of a taxadiene synthase enzyme and a geranylgeranyl diphosphate synthase (GGPPS) enzyme in cells and the production of terpenoids.
    Type: Application
    Filed: July 17, 2013
    Publication date: January 23, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: PARAYIL K. AJIKUMAR, GREGORY STEPHANOPOULOS, HENG PHON TOO
  • Tailored oils produced from recombinant oleaginous microorganisms
    Patent number: 8633012
    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: October 11, 2012
    Date of Patent: January 21, 2014
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky, Xinhua Zhao, Riyaz Bhat
  • TRANSGENIC ALGAE FOR DELIVERING ANTIGENS TO AN ANIMAL
    Publication number: 20140017272
    Abstract: Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.
    Type: Application
    Filed: January 2, 2013
    Publication date: January 16, 2014
    Applicants: The Ohio State University Research Foundation, ALGAEOMICS LLC
    Inventor: Richard T. Sayre
  • Photosynthetic organisms and compositions and methods of generating same
    Patent number: 8629259
    Abstract: An isolated polynucleotide is provided. The isolated polynucleotide comprising a nucleic acid sequence encoding a polypeptide of a Type II reaction center of a photosynthetic organism, the nucleic acid sequence being capable of imparting the type II reaction center with an activity under a temperature range different than that of the type II reaction center endogenous to the photosynthetic organism. Also provided are methods of using the sequences for generating photosynthetic organisms or tailor-made thermotolerance.
    Type: Grant
    Filed: July 22, 2007
    Date of Patent: January 14, 2014
    Assignee: Yeda Research and Development Co. Ltd.
    Inventors: Avigdor Scherz, Oksana Shlyk-Kerner, Ilan Samish, David Kaftan, Jorge Dinamarca
  • Genetically Modified Photoautotrophic Ethanol Producing Host Cells, Method For Producing The Host Cells, Constructs For The Transformation Of The Host Cells, Method For Testing A Photoautotrophic Strain For A Desired Growth Property And Method Of Producing Ethanol Using The Host Cells
    Publication number: 20140011264
    Abstract: The invention provides genetically modified photoautotrophic ethanol producing host cells. Ethanol production is controlled by an inducible promoter. Promoters utilized may be endogenous or heterologous, and genetic modification may be obtained by extrachromosomal means or chromosomal insertion.
    Type: Application
    Filed: July 12, 2013
    Publication date: January 9, 2014
    Applicant: Algenol Biofuels Inc.
    Inventors: Ulf Duhring, Heike Enke, Dan Kramer, Craig Smith, Robert Paul Woods, Kerstin Baier, Christine Oesterhelt
  • MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS
    Publication number: 20140004580
    Abstract: This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl-acyl carrier protein reductase (acyl-ACP reductase). These microorganisms may optionally overexpress one or more fatty acid synthesis proteins such as ACP and ACCase, and/or one or more polypeptides associated with glycogen breakdown. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids or wax esters and/or synthesizing triglycerides.
    Type: Application
    Filed: December 19, 2011
    Publication date: January 2, 2014
    Applicant: Matrix Genetics, LLC
    Inventors: James Roberts, Fred Cross, Brett K. Kaiser
  • Methods and Compositions for the Recombinant Biosynthesis of N-Alkanes
    Publication number: 20140005439
    Abstract: The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into n-alkanes, and in particular the use of such organisms for the commercial production of n-alkanes and related molecules.
    Type: Application
    Filed: June 10, 2013
    Publication date: January 2, 2014
    Applicant: JOULE UNLIMITED TECHNOLOGIES, INC.
    Inventors: Nikos Basil Reppas, Christian Perry Ridley
  • Genetic Switches for Butanol Production
    Publication number: 20140004526
    Abstract: The invention relates to suitable screening strategies for evaluating various candidate promoters for differential gene expression during the propagation and production phases of a fermentation process. The invention also relates to recombinant host cells that comprise identified promoter nucleic acid sequences and methods for producing fermentation products employing the same.
    Type: Application
    Filed: December 28, 2012
    Publication date: January 2, 2014
    Applicant: Butamax™ Advanced Biofuels LLC
    Inventor: Butamax™ Advanced Biofuels LLC
  • MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS
    Publication number: 20130344549
    Abstract: This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism.
    Type: Application
    Filed: December 19, 2011
    Publication date: December 26, 2013
    Applicant: Targeted Growth, Inc.
    Inventors: James Roberts, Fred Cross, Margaret Mary McCormick, Ernesto Javier Munoz, Brett K. Kaiser, Michael Carleton
  • Modified Fluorescent Proteins and Methods for Using Same
    Publication number: 20130344591
    Abstract: Nucleic acid molecules encoding improved fluorescent mutants of the mKate2 protein, variants and derivatives thereof are provided, as well as proteins and peptides encoded by these nucleic acids. Also provided are proteins that are substantially similar to, or derivatives, homologues, or mutants of, the above-referenced specific proteins. Also provided are fragments of the nucleic acids and the peptides encoded thereby, as well as antibodies specific to the proteins and peptides of the invention. In addition, host-cells, stable cell lines and transgenic organisms comprising above-referenced nucleic acid molecules are provided. The subject protein and nucleic acid compositions find use in a variety of different applications and methods, particularly for labeling of biomolecules, cells or cell organelles. Finally, kits for use in such methods and applications are provided.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 26, 2013
    Applicant: EVROGEN JSC
    Inventors: Sergey A. Lukyanov, Dmitry M. Chudakov
  • Mutant ?-5 desaturases mutated in the heme-binding motif (HPGG)
    Patent number: 8614075
    Abstract: The present invention relates to mutant ?5 desaturases, which have the ability to convert dihomo-?-linolenic acid [DGLA; 20:3 ?-6] to arachidonic acid [ARA; 20:4 ?-6] and/or eicosatetraenoic acid [ETA; 20:4 ?-3] to eicosapentaenoic acid [EPA; 20:5 ?-3] and which possess at least one mutation within the HPGG motif of the cytochome b5-like domain. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding ?5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant ?5 desaturases in oleaginous yeast, are disclosed.
    Type: Grant
    Filed: January 8, 2013
    Date of Patent: December 24, 2013
    Assignee: E I du Pont de Nemours and Company
    Inventors: Quinn Qun Zhu, Dana M. Walters Pollak
  • Processes for Producing Polyunsaturated Fatty Acids in Transgenic Organisms
    Publication number: 20130340103
    Abstract: The present invention relates to polynucleotides from Ostreococcus lucimarinus which code for desaturases and elongases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides, and to the polypeptides encoded by the polynucleotides. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 19, 2013
    Applicant: BASF Plant Science GmbH
    Inventors: Jörg Bauer, Tom Wetjen
  • IDENTIFICATION OF ISOPRENE SYNTHASE VARIANTS WITH IMPROVED PROPERTIES FOR THE PRODUCTION OF ISOPRENE
    Publication number: 20130330796
    Abstract: The invention provides for compositions and methods for producing isoprene using isoprene synthase variants with improved properties.
    Type: Application
    Filed: March 13, 2013
    Publication date: December 12, 2013
    Applicants: The Goodyear Tire & Rubber Company, DANISCO US INC.
    Inventors: Zachary Q. BECK, David A. ESTELL, Jeffrey V. MILLER, James NGAI, Christopher L. RIFE, Derek H. WELLS
  • Tailored Oils
    Publication number: 20130331584
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Application
    Filed: July 12, 2013
    Publication date: December 12, 2013
    Applicant: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • ENHANCER OF CELL DIVISION
    Publication number: 20130333074
    Abstract: The present invention relates to a polypeptide (BIG1) and variants thereof capable of enhancing the rate of cell-division of a microorganism or plant cell, as well as nucleic acid molecules encoding said polypeptides, vectors comprising said nucleic acid molecules and host cells transformed or transfected with said vectors and expressing said polypeptides. The BIG1 polypeptide which has been identified in the marine centric diatom Thalassiosira pseudonana, variants thereof and nucleic acids encoding these may be used in methods of enhancing the rate of cell-division of microorganisms, plant cells or plants which produce useful sub stances or exhibit useful properties, to increase the yield thereof.
    Type: Application
    Filed: February 10, 2012
    Publication date: December 12, 2013
    Applicants: University of Washington through its Center for Commercialization, University of East Anglia
    Inventors: Thomas Mock, Rachel Elizabeth Hipkin
  • PROMOTERS AND TERMINATORS FOR USE IN EUKARYOTIC CELLS
    Publication number: 20130323780
    Abstract: The present invention provides novel promoter and terminator sequences for use in heterologous gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a heterologous gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the heterologous gene; and methods for identifying promoters.
    Type: Application
    Filed: June 1, 2012
    Publication date: December 5, 2013
    Applicant: EXXONMOBIL RESEARCH AND ENGINEERING COMPANY
    Inventors: JANE C. SCHNEIDER, BO LIU, REKHA SESHADRI
  • METHODS AND COMPOSITIONS FOR THE PRODUCTION OF EXTREMOPHILE ENZYMES FROM GREEN MICROALGAE AND CYANOBACTERIA
    Publication number: 20130323803
    Abstract: The present invention relates to compositions and methods for stable transformation of green microalgae and for production of transgenic green microalgae and/or cyanobacteria that produce extremophile enzymes as co-products during the growth of the green microalgae and/or cyanobacteria for lipid biofuel production. Thus, the present invention provides nucleic acid constructs and methods of transformation useful in the production of stably transformed green microalgae and/or cyanobacteria expressing extremophile enzymes in combination with lipid production for biofuel.
    Type: Application
    Filed: February 3, 2012
    Publication date: December 5, 2013
    Applicant: North Carolina State University
    Inventors: Amy Michele Grunden, Heike Inge Ada Sederoff
  • Tailored Oils
    Publication number: 20130323382
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.
    Type: Application
    Filed: July 12, 2013
    Publication date: December 5, 2013
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Tailored Oils
    Publication number: 20130323823
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Application
    Filed: July 12, 2013
    Publication date: December 5, 2013
    Applicant: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Production of Branched-Chain Alcohols by Photosynthetic Microorganisms
    Publication number: 20130316415
    Abstract: The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses.
    Type: Application
    Filed: May 21, 2013
    Publication date: November 28, 2013
    Applicant: Synthetic Genomics, Inc.
    Inventor: Synthetic Genomics, Inc.
  • CONTROL OF PATHOGENS AND PARASITES
    Publication number: 20130315883
    Abstract: The present invention relates to the genetic control of parasites and pathogens via the expression of silencing RNA in transgenic plants, including microalgae. In one aspect, the invention exploits the ability of plants to express the silencing RNA in a form within chloroplasts that is efficiently taken up, after ingestion where it can act to suppress the expression of target genes within the pathogen or parasite.
    Type: Application
    Filed: October 24, 2011
    Publication date: November 28, 2013
    Inventors: Richard Sayre, Anil Kumar
  • Compositions and Methods for Altering Tocotrienol Content
    Publication number: 20130316457
    Abstract: The invention provides isolated nucleic acids and their encoded polypeptides that alter tocol content in seeds. The invention further provides expression cassettes, host cells and transformed plants containing the nucleic acids. The present invention further provides methods for altering tocol content in seeds.
    Type: Application
    Filed: May 7, 2013
    Publication date: November 28, 2013
    Applicant: E.I. DuPont de Nemours and Company
    Inventors: Edgar B. Cahoon, Sean J. Coughlan, Rebecca E. Cahoon, Karlene H. Butler
  • PHOTOSYNTHETIC MICROORGANISMS EXPRESSING THERMOSTABLE LIPASE
    Publication number: 20130316458
    Abstract: The present invention encompasses a photosynthetic microorganism that produces biofuels and biofuel precursors.
    Type: Application
    Filed: May 20, 2013
    Publication date: November 28, 2013
    Applicant: The Arizona Board of Regents for and on Behalf of Arizona State University
    Inventors: Roy Curtiss, Xinyao Liu
  • Tailored Oils
    Publication number: 20130316410
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Application
    Filed: April 18, 2013
    Publication date: November 28, 2013
    Applicant: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Tailored oils produced from recombinant heterotrophic microorganisms
    Patent number: 8592188
    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Grant
    Filed: May 27, 2011
    Date of Patent: November 26, 2013
    Assignee: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
  • HUWENTOXIN-IV VARIANTS AND METHODS OF USE
    Publication number: 20130310324
    Abstract: The present invention relates to Huwentoxin-IV variants, polynucleotides encoding them, and methods of making and using the foregoing.
    Type: Application
    Filed: March 15, 2013
    Publication date: November 21, 2013
    Applicant: JANSSEN BIOTECH, INC.
    Inventors: Mack Flinspach, Michael Hunter, Yi Liu, Robert Neff, Alan Wickenden, Alan Gibbs
  • METHOD OF MICROALGAL CHLOROPLAST TRANSFORMATION USING FUNCTIONAL SELECTION IN THE ABSENCE OF ANTIBIOTICS
    Publication number: 20130302812
    Abstract: This invention relates to a new method of selecting for transgene transformants in the absence of antibiotic selective pressure, where the method is based on recovery of function.
    Type: Application
    Filed: November 7, 2011
    Publication date: November 14, 2013
    Applicant: The Regents of the University of California
    Inventors: Anastasios Melis, Hsu-Ching Chen Wintz
  • Pre-Ligand assembly domain of the IL-17 receptor
    Publication number: 20130302886
    Abstract: The invention provides isolated Pre-Ligand Assembly Domain (PLAD) polypeptides which inhibit multimerization of a receptor complex comprising an IL-17R family member. Related PLAD-binding polypeptides, chimeric proteins, conjugates, nucleic acids, vectors, host cells, and methods are provided herein.
    Type: Application
    Filed: May 8, 2013
    Publication date: November 14, 2013
    Applicants: RESEARCH FOUNDATION OF THE STATE UNIVERSITY OF NEW YORK, AMGEN INC.
    Inventors: Sarah L. GAFFEN, Fang Shen, Walter Hanel, Jill Kramer, James P. Malone, Michael Wittekind, Raymond Paxton
  • COMPOSITIONS AND METHODS RELATING TO MOUSE PAPILLOMA VIRUS
    Publication number: 20130295125
    Abstract: Methods and compositions for detecting MusPV, also known as MmuPv1, infection of a rodent subject are described according to aspects of the present invention. In specific aspects, the present invention relates to assays for detecting MusPV infection of a rodent subject; vaccine compositions for inducing an immunological response against MusPV in a rodent subject; methods of inducing an immunological response to MusPV in a rodent subject; isolated MusPV protein; isolated antibodies which specifically binds to an MusPV protein, a fragment or variant thereof; isolated recombinantly expressed MusPV proteins; expression constructs comprising a nucleic acid encoding an MusPV protein; host cells comprising the expression construct; and hybridoma cell lines expressing an anti-MusPV monoclonal antibody specific for MusPV.
    Type: Application
    Filed: March 14, 2013
    Publication date: November 7, 2013
    Applicants: UNIVERSITY OF LOUISVILLE RESEARCH FOUNDATION, THE JACKSON LABORATORY
    Inventors: John P. Sundberg, Joongho Joh, A. Bennett Jenson, Shin-Je Ghim
  • COBALAMIN ACQUISITION PROTEIN AND USE THEREOF
    Publication number: 20130296257
    Abstract: The present invention relates to a cobalamin acquisition protein, compositions containing the cobalamin acquisition protein, and the use of such compositions.
    Type: Application
    Filed: April 30, 2013
    Publication date: November 7, 2013
    Inventor: Woods Hole Oceanographic Institution
  • ISOPRENE SYNTHASE VARIANTS WITH IMPROVED SOLUBILITY FOR PRODUCTION OF ISOPRENE
    Publication number: 20130295632
    Abstract: The present invention provides methods and compositions of variant polypeptides having isoprene synthase activity with improved solubility. In particular, the present invention provides isoprene synthase variant for increased isoprene production in recombinant host cells.
    Type: Application
    Filed: October 26, 2012
    Publication date: November 7, 2013
    Applicants: The Goodyear Tire & Rubber Company, Danisco US Inc.
    Inventors: Christopher L. RIFE, Derek H. WELLS
  • RECOMBINANT LIGNOCELLULOSE DEGRADATION ENZYMES FOR THE PRODUCTION OF SOLUBLE SUGARS FROM CELLULOSIC BIOMASS
    Publication number: 20130288310
    Abstract: The invention relates to C1 lignocellulose degradation enzyme nucleic acid and protein sequences and expression of recombinant C1 lignocellulose degradation enzymes. The invention provides methods for degrading a cellulosic biomass by contacting the biomass with a recombinant C1 lignocellulose degradation enzyme of the invention.
    Type: Application
    Filed: August 22, 2011
    Publication date: October 31, 2013
    Applicant: Codexis, Inc
    Inventors: Louis Clark, Dipnath Baidyaroy
  • INCREASED YIELDS OF PHAS FROM HYDROGEN FEEDING AND DIVERSE CARBON FIXATION PATHWAYS
    Publication number: 20130288317
    Abstract: Disclosed are methods including organisms genetically engineered to make useful products when grown on glucose as a carbon source. The organisms are genetically engineered to produce various useful products such as polyhydroxyalkanoates (PHA) monomers, polymers, and copolymers, diols, alcohols, and other useful chemicals.
    Type: Application
    Filed: March 14, 2013
    Publication date: October 31, 2013
    Inventors: Thomas M. Ramseier, Christopher W.J. McChalicher
  • COMPOSITIONS AND METHODS FOR MODULATING THE SENSITIVITY OF CELL TO AHAS INHIBITORS
    Publication number: 20130288377
    Abstract: Methods and materials useful for modulating the sensitivity of cells to an inhibitor of acetohydroxyacid synthase (AHAS) are disclosed. For example, nucleic acid molecules encoding AHAS large subunits are disclosed as well as methods for using such nucleic acid molecules to transform microbial cells and plant cells, and to confer modulated sensitivity to AHAS-inhibiting compounds onto such cells. Further provided are materials and methods useful for modulating growth, development, activity, and characteristics of host cells and organisms.
    Type: Application
    Filed: April 26, 2013
    Publication date: October 31, 2013
    Applicant: Synthetic Genomics, Inc.
    Inventors: Michele M. Champagne, Jun Urano
  • BIOFUEL AND CHEMICAL PRODUCTION BY RECOMBINANT MICROORGANISMS VIA FERMENTATION OF PROTEINACEOUS BIOMASS
    Publication number: 20130288325
    Abstract: Provided herein are metabolically modified microorganisms characterized by having an increased keto-acid flux when compared with the wild-type organism and comprising at least one polynucleotide encoding an enzyme that when expressed results in the production of a greater quantity of a chemical product when compared with the wild-type organism. The recombinant microorganisms are useful for producing a large number of chemical compositions from various nitrogen containing biomass compositions and other carbon sources.
    Type: Application
    Filed: November 3, 2011
    Publication date: October 31, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: James C. Liao, Kwang Myung Cho, Yajun Yan, Yixin Huo
  • Novel Fatty Acid Desaturases, Elongases, Elongation Components and Uses Thereof
    Publication number: 20130291228
    Abstract: The invention provides isolated nucleic acid molecules which encodes a novel fatty acid desaturase, KCS, KCR, DH and ECR from Nannochloropsis oculata. The invention also provides recombinant expression vectors containing desaturase, KCS, KCR, DH and ECR nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA.
    Type: Application
    Filed: October 19, 2011
    Publication date: October 31, 2013
    Applicant: BASF PLANT SCIENCE COMPANY GmbH
    Inventors: Toralf Senger, Jörg Bauer, Laurent Marty
  • Materials and methods for the production of biodiesel
    Patent number: 8569021
    Abstract: The subject invention provides novel microorganisms comprising polynucleotides and polypeptides encoding a methyltransferase. The subject invention provides novel methods for the production of biodiesel.
    Type: Grant
    Filed: December 14, 2010
    Date of Patent: October 29, 2013
    Assignee: University of Tennessee Research Foundation
    Inventors: Feng Chen, Nan Zhao
  • Reagents and Methods for Cyanobacterial Production of Bioplastics and Biomaterials
    Publication number: 20130280772
    Abstract: The present invention provides reagents and methods for biomaterial production from cyanobacteria.
    Type: Application
    Filed: June 6, 2013
    Publication date: October 24, 2013
    Inventors: Hatem Mohammed, Wim Vermaas
  • CELL SYSTEMS AND METHODS FOR IMPROVING FATTY ACID SYNTHESIS BY EXPRESSION OF DEHYDROGENASES
    Publication number: 20130280793
    Abstract: The invention relates to methods for producing lipids such as fatty acid products in recombinant host cells engineered to express a non-native gene encoding a dehydrogenase. The recombinant microorganisms are able to proliferate at a higher rate as compared with microorganisms that do not express a non-native dehydrogenase gene, and cultures of microorganisms that are engineered for lipid production and that express a non-native dehydrogenase produce more lipid than cultures of control microorganisms that do not include a non-native dehydrogenase gene.
    Type: Application
    Filed: April 23, 2012
    Publication date: October 24, 2013
    Applicant: ExxonMobil Research and Engineering Company
    Inventors: Robert Christopher Brown, Jennifer Coppersmith, Prachee Prakash, Srividya Akella, Rekha Seshadri
  • Transgenic Algae with Enhanced Oil Expression
    Publication number: 20130280770
    Abstract: The present invention provides transgenic algal cells that produce an increased amount of oil, methods of making transgenic algal cells, and methods of obtaining biofuel from the transgenic algal cells.
    Type: Application
    Filed: April 22, 2011
    Publication date: October 24, 2013
    Applicant: Senesco Technologies, Inc.
    Inventors: Holly Loucas, Tzann-wei Wang, John Thompson
  • Methods and Compositions for Targeting Heterologous Integral Membrane Proteins to the Cyanobacterial Plasma Membrane
    Publication number: 20130280780
    Abstract: This disclosure pertains to the functional localization of heterologous integral plasma membrane proteins (HIPMPs) lacking cleavable signal sequences into the plasma membrane (PM) of cyanobacterial hosts, e.g., JCC138 (Synechococcus sp. PCC 7002) or an engineered derivative thereof. More specifically, the disclosure provides chimeric integral plasma membrane proteins comprising pseudo leader sequences (PLSs) that promote increased hydrocarbon (e.g., alkane) export capabilities when expressed in a photosynthetic organism, e.g., a cyanobacterium.
    Type: Application
    Filed: June 18, 2013
    Publication date: October 24, 2013
    Inventor: Nikos B. Reppas
  • Hyperphotosynthetic Organisms
    Publication number: 20130273613
    Abstract: The present disclosure identifies pathways and mechanisms to confer improved industrial fitness on engineered organisms. It also discloses engineered organisms having improved industrial fitness. Synthetic biologic engineering modules are disclosed that provide for light capture, carbon dioxide fixation, NADH production, NADPH production, thermotolerance, pH tolerance, flue gas tolerance, salt tolerance, nutrient independence and near infrared absorbance. The disclosed engineered organisms can include one or more of these modules. Also provided are methods of using the engineered organism to produce carbon-based products of interest, biomass or pharmaceutical agents.
    Type: Application
    Filed: March 12, 2013
    Publication date: October 17, 2013
    Inventors: Eric James Devroe, Sriram Kosuri, David Arthur Berry, Noubar Boghos Afeyan, Frank Anthony Skraly, Dan Eric Robertson, Brian Green, Christian Perry Ridley
  • ENHANCED GENE EXPRESSION IN ALGAE
    Publication number: 20130273638
    Abstract: The invention provides eukaryotic unicellular algae engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.
    Type: Application
    Filed: June 25, 2013
    Publication date: October 17, 2013
    Inventors: George Oyler, Julian Rosenberg
  • TAL-EFFECTOR ASSEMBLY PLATFORM, CUSTOMIZED SERVICES, KITS AND ASSAYS
    Publication number: 20130274129
    Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.
    Type: Application
    Filed: April 4, 2013
    Publication date: October 17, 2013
    Inventors: Federico KATZEN, Jonathan Chesnut, Frank Notka, Matthias Arnold, Kevin Hoff, Stephen Whiteside
  • Ethanol Production in Microorganisms
    Publication number: 20130252300
    Abstract: The present disclosure relates to methods and compositions for engineering photoautotrophic organisms to convert carbon dioxide and light into fatty acid esters and other molecules, including biofuels. The molecules are then secreted by the organism into a growth medium.
    Type: Application
    Filed: May 6, 2013
    Publication date: September 26, 2013
    Applicant: Joule Unlimited Technologies, Inc.
    Inventors: Brian D. Green, Nikos Basil Reppas, Dan Eric Robertson
  • SECRETION OF RECOMBINANT POLYPEPTIDES IN THE EXTRACELLULAR MEDIUM OF DIATOMS
    Publication number: 20130244265
    Abstract: A transformed diatom includes a nucleic acid sequence operatively linked to a promoter, wherein the nucleic acid sequence encodes an amino acid sequence including (i) an heterologous signal peptide and (ii) a polypeptide, the heterologous signal peptide leading to the secretion of the polypeptide in the extracellular medium of the transformed diatom; a method for producing a polypeptide which is secreted in the extracellular medium, the method including the steps of (i) culturing a transformed diatom, (ii) harvesting the extracellular medium of the culture and (iii) purifying the secreted polypeptide in the extracellular medium; and use of the transformed diatom for the secretion of a polypeptide in the extracellular medium.
    Type: Application
    Filed: October 20, 2011
    Publication date: September 19, 2013
    Applicant: ALGENICS
    Inventors: Alexandre Lejeune, Rémy Michel, Jean-Paul Cadoret, Aude Carlier