Algae, Media Therefor Patents (Class 435/257.1)
  • Patent number: 10711232
    Abstract: The invention relates to a bioreactor comprising a tank containing a culture medium in which a cell culture composed of algae cells is dispersed. The cell culture of algae has a concentration greater than 0.1 g/L in the culture medium, and each algal cell has a minimum absorption in a specific range of wavelengths of light. The bioreactor comprises a light source that is capable of emitting incident light in the direction of the tank, 60% of the photons of the incident light having a wavelength which is included in said specific range of wavelengths of light. The invention also relates to the use of the bioreactor for the production of biomass.
    Type: Grant
    Filed: March 18, 2016
    Date of Patent: July 14, 2020
    Assignees: Inria Institut National De Recherche En Informatique Et En Automatique, Centre National De La Recherche Scientifique (C.N.R.S.), Universite Pierre Et Marie Curie (Paris 6)
    Inventors: Francis Mairet, Olivier Bernard, Antoine Sciandra, Eric Pruvost, Charlotte Combe
  • Patent number: 10370687
    Abstract: It is an object of the present invention to provide the eukaryotic microalgae, which have been genetically modified such that larger amounts of assimilation products produced by photosynthesis are directed to the synthesis of triglyceride (=triacylglycerol; TAG), and specifically, the present invention relates to a genetically modified strain of eukaryotic microalgae, in which a gene encoding an AGL1 protein is highly expressed, or a gene encoding an FAT1 protein and/or a gene encoding a DGAT2 protein are further highly expressed, as well as the gene encoding an AGL1 protein, wherein TAG productivity is improved in comparison to the parent strain thereof.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: August 6, 2019
    Assignees: CHUO UNIVERSITY, DENSO CORPORATION
    Inventors: Shigeaki Harayama, Yoko Ide, Jun Abe, Yuki Kasai, Norihide Kurano
  • Patent number: 10173913
    Abstract: A process of growing a culture of cyanobacteria or algae using chitin or chitosan as a source of nitrogen for photosynthetic growth is described. This process can be used to remove pollutants from nitrogen-deficient natural waters or wastewaters including buchu mercaptan production wastewater. Biomass that results from photosynthetic growth on chitin can be used, either as whole cells or the isolated components of the cells, for a large variety of commercial purposes.
    Type: Grant
    Filed: June 1, 2017
    Date of Patent: January 8, 2019
    Inventors: Carrine Blank, Nancy W. Hinman
  • Patent number: 10160945
    Abstract: The invention relates to the field of mass cultivation of microalgae and specifically relates to a horizontal submerged cover type carbon supplement device and carbon supplement method for cultivating microalgae in an open pond. The horizontal submerged cover type carbon supplement device for cultivating microalgae in an open pond, comprises: one or more than two covers and gas distributors, wherein the gas distributor is arranged at the bottom of the open pond and the cover is arranged above the gas distributor; the cover, in form of a cylinder with openings at both ends, is arranged along the flow direction of the culture solution, the two ends of the cover being a culture solution inlet and a culture solution outlet respectively; the gas distributor is kept away from the culture solution outlet of the cover by more than 40 cm.
    Type: Grant
    Filed: May 7, 2012
    Date of Patent: December 25, 2018
    Assignee: Institute of Process Engineering, Chinese Academy of Sciences
    Inventors: Wei Cong, Ming Liu, Xia Wu, Shumei Wen, Dongmei Zhang, Zhongliang Sun
  • Patent number: 10119095
    Abstract: A method for processing algae-based products using flue gas heat includes mixing flue gas with water, receiving a heated gas in a first enclosure of a heat exchanger, receiving an algal paste in a second enclosure of the heat exchanger, introducing an organic solvent to the algal paste, extracting an algal oil and a plurality of algal shells from the algal paste by dissipating heat from the first enclosure to the second enclosure; and extracting the algal oil from the organic solvent and the algal shells.
    Type: Grant
    Filed: January 31, 2017
    Date of Patent: November 6, 2018
    Assignee: T2 Energy, LLC
    Inventors: Mark Randall, Mark Olson, Elmar Schmid
  • Patent number: 10059968
    Abstract: The present disclosure relates to a method for increasing lipid content in microorganisms. The method comprises decreasing the expression of molecules involved in the protein synthesis to decrease protein synthesis and thereby increase lipid synthesis in the microorganisms. The present disclosure also provides a modified microorganism having increased lipid content.
    Type: Grant
    Filed: May 28, 2015
    Date of Patent: August 28, 2018
    Assignee: RELIANCE INDUSTRIES LIMITED
    Inventors: Gautam Das, Santanu Dasgupta, Venkatesh Prasad, Vinodhkumar Vijayakumar, Pranali Deore, Kannadasan Kaliyamoorthy, Sujata Kumari
  • Patent number: 10012574
    Abstract: Provided herein is a method comprising one or more of the following steps: (a) lysing cells of a biological sample and contacting the biological sample with an amount of ionic liquid sufficient to denature intracellular metabolic enzymes in the biological sample to produce a contacted cellular sample; (b) mixing the contacted cellular sample with an organic solvent to produce an ionic liquid-organic solvent composition; (c) mixing the contacted cellular sample with the organic solvent to produce a dispersed microdroplet ionic liquid-organic solvent composition; (d) contacting the ionic liquid-organic solvent composition with an ion exchange composition to produce a second ionic liquid-organic solvent composition; (d) separating the ionic liquid from the organic solvent; and (e) extracting metabolites from the ionic liquid. Kits and systems for practicing the subject methods are also provided.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: July 3, 2018
    Assignee: Agilent Technologies, Inc.
    Inventor: James Alexander Apffel, Jr.
  • Patent number: 10006071
    Abstract: Methods are provided to control the harvesting of desired metabolic byproducts from hypersaline photosynthetic algae. Methods are presented to control secretion of said metabolic products in monocultures and co-cultures after accumulation of byproducts. The metabolic byproduct secretion is controlled by controlling caspase activity.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: June 26, 2018
    Assignee: Institute for System Biology
    Inventors: Nitin S. Baliga, Monica V. Orellana, Kenia Whitehead, W. Lee Pang
  • Patent number: 9944958
    Abstract: The purpose of the present invention is to effectively accumulate triacylglycerol in algae cells, the present invention providing a method for introducing a triacylglycerol synthetase gene, a phosphorus starvation-inducible promoter, and a 3? untranslated region into algae.
    Type: Grant
    Filed: March 12, 2015
    Date of Patent: April 17, 2018
    Assignee: TOKYO INSTITUTE OF TECHNOLOGY
    Inventors: Masako Iwai, Hiroyuki Ohta, Mie Shimojima
  • Patent number: 9932554
    Abstract: Bioreactors and methods for cultivating microalgae are provided herein. The bioreactor and methods include features and modifications to improve heterotrophic growth efficiency by providing a light signal.
    Type: Grant
    Filed: September 17, 2010
    Date of Patent: April 3, 2018
    Assignee: PHYCOIL BIOTECHNOLOGY INTERNATIONAL, INC.
    Inventors: Chung-Soon Im, Jane Kim
  • Patent number: 9919978
    Abstract: The present invention discloses a selenium-doped nano-silica sol capable of both inhibiting the absorption and accumulation of heavy metal in rice and producing a selenium-rich rice, and a preparation method thereof. The selenium-doped nano-silica sol of the present invention is prepared mainly by using a nano-silica sol as a carrier, sodium selenite and the like as a raw material, vitamin C and the like as a reducing agent, and polyvinylpyrrolidone and the like as an emulsifier, and doping and dispersing selenium in a specific ratio in a silica sol so as to form a selenium-doped nano-silica sol with a special structure and function. The selenium-doped nano-silica sol prepared according to the invention has high stability and high concentration, and is uniform and transparent, and has a silica content of up to 20% or more, a selenium content of up to 1% or more, and low impurity content.
    Type: Grant
    Filed: October 10, 2014
    Date of Patent: March 20, 2018
    Assignee: Guangdong Institute of Eco-Environmental Science & Technology
    Inventors: Chuanping Liu, Fangbai Li, Jianghu Cui, Xianghua Xu
  • Patent number: 9890402
    Abstract: The present invention relates to the continuous process of sequestration of nutrients from waste effluents released from gas fermentation plants or green-house gases emission managing plants by novel Thraustochytrids. Particularly this invention relates to the methods and systems to enhance sequestration rate and productivity of the process. This invention also relates to rapid biotransformation of nutrients present in waste effluents into high value omega-3 fatty acids like Docosahexaenoic acid (DHA), Docosapentaenoic acid (DPA), Eicosapentaenoic acid (EPA) and lipids for biodiesel. This disclosure is about means of processing of waste streams and producing value added products out of it.
    Type: Grant
    Filed: January 22, 2016
    Date of Patent: February 13, 2018
    Assignees: INDIAN OIL CORPORATION LIMITED, DEPARTMENT OF BIOTECHNOLOGY
    Inventors: Anshu Shankar Mathur, Dilip Singh, Preeti Mehta, Ravi Prakash Gupta, Deepak Kumar Tuli
  • Patent number: 9850555
    Abstract: The present application is generally directed to separation and recovery of rare earths using biomass, liposomes, and/or other materials. In some embodiments, a composition comprising rare earths is exposed to biomass, where some of the rare earths are transferred to the biomass, e.g., via absorption. The composition may then be separated from the biomass. A solution may be exposed to the biomass thereby enriching the solution in one or more rare earths, relative to other rare earths in the biomass. The solution and the biomass may then be separated, and the rare earths recovered from the solution. In some cases, this process may be repeated with different solutions, which may result in different solutions enriched in various rare earths. Similar processes may be used to separate the rare earths from thorium and uranium. Liposomes may be used instead of and/or in addition to biomass.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: December 26, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: William Daley Bonificio, David Clarke
  • Patent number: 9783581
    Abstract: An object of the present invention is to construct a production system that enables efficient production of organic acids using blue-green algae, which are photosynthetic microorganisms, by utilizing carbon dioxide and thereby increasing an amount of organic acids produced. The present invention relates to blue-green algae overexpressing a clock protein gene and a method for producing organic acids by culturing the blue-green algae.
    Type: Grant
    Filed: January 29, 2015
    Date of Patent: October 10, 2017
    Assignee: Riken
    Inventors: Takashi Osanai, Masami Hirai, Kazuki Saito, Hiroko Iijima, Ayuko Kuwahara
  • Patent number: 9643867
    Abstract: A waste processing system includes: an input module for converting input organic waste into processed input waste; a digester module, coupled to the input module, for generating biogas and digester effluent from the processed input waste, including: a digester tank; baffles affixed within the digester tank; a digester process solution in the digester tank including, hydrolytic bacteria, acidogenic bacteria, acetogenic bacteria, methanogenic bacteria, or a combination thereof, for converting the processed input waste into the biogas and the digester effluent; and an output module, coupled to the digester module, for collecting the biogas and digester effluent after a residence period.
    Type: Grant
    Filed: June 24, 2014
    Date of Patent: May 9, 2017
    Assignee: Biovessel Technologies Inc.
    Inventors: Jeff Brooks, Chaiyos Siripoke
  • Patent number: 9593067
    Abstract: The present invention relates to a method for the recovery of intracellular components of materials of a biological origin, such as yeasts, algae, bacteria and/or mildews, after fermentation/cultivation, which comprises a thermal lysing treatment via nebulization and the use of a nebulization solvent capable of extracting said intracellular components once the nebulization has been completed.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: March 14, 2017
    Assignee: ENI S.p.A.
    Inventors: Roberta Miglio, Alfredo Montini
  • Patent number: 9464304
    Abstract: Methods are disclosed for cultivation of oleaginous microorganisms on feedstocks including depolymerized cellulosic material such as corn stover, Miscanthus, forage sorghum, sugar beet pulp and sugar cane bagasse. Also disclosed are methods of manufacturing novel triglyceride oil compositions using cellulosic feedstocks, including oil compositions comprising fatty acids such as those having chain lengths of 8, 10, 12, and 14 carbon atoms.
    Type: Grant
    Filed: November 30, 2009
    Date of Patent: October 11, 2016
    Assignee: TerraVia Holdings, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Karen Espina, George Rudenko, Penelope Chua
  • Patent number: 9447374
    Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises detecting the contamination and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: September 20, 2016
    Assignee: HELIAE DEVELOPMENT LLC
    Inventors: Laura Carney, Kristine Sorensen
  • Patent number: 9447373
    Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with hydrogen peroxide and salt are described herein. The method comprises dosing the culture comprising a concentration of salt with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: September 20, 2016
    Assignee: HELIAE DEVELOPMENT LLC
    Inventors: Laura Carney, Kristine Sorensen
  • Patent number: 9441252
    Abstract: The present invention provides a cost effective biotechnological process for production of bio-fuels from isolated and characterized microalgae. The algal strains used in the present invention having higher biomass, higher lipid productivity, higher pH and temperature tolerance are selected from the group consisting of Chlorella vulgaris iOC-1, Chlorella vulgaris iOC-2, Chlorella kessleri, Botrococcus bruni, Dunaliella salina and Nannochloris oculat or a combination thereof having 95-100% similarity with 18s ribosomal nucleic acids nucleotide sequences (rDNA) given for Seq. ID I, Seq. ID 2, Seq. ID 3, Seq. ID 4, Seq. ID 5 and Seq. ID 6. The present process of bio-fuel production comprises the steps of producing lipid from green algae in bioreactors by various novel steps and extracting oil from dried algal cells and ultimately producing biodiesel by transesterification of the said extracted oil.
    Type: Grant
    Filed: October 11, 2012
    Date of Patent: September 13, 2016
    Assignee: INDIAN OIL CORPORATION LTD.
    Inventors: Mahendra Pratap Singh, Manoj Kumar, Dheer Singh, Deepak Kumar Tuli, Ravinder Kumar Malhotra
  • Patent number: 9428425
    Abstract: The present invention comprises methods and compositions comprising a microalgae consortium. A composition comprises at least one genera of microalgae flocculated by Paenibacillus polymyxa strain, Strain 2, deposited under the Budapest Treaty as ATCC Accession No. PTA-12841. Methods of treating the soil comprise adding a composition of the present invention to soil.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: August 30, 2016
    Assignee: Core Intellectual Properties Holdings, LLC
    Inventors: Luke Blotsky, Karin Hastings, Derex Q Zellars
  • Patent number: 9416346
    Abstract: A method of damaging cell structure of an aquatic substance includes: providing an aquatic substance raw material, where the aquatic substance raw material includes an aquatic substance; adjusting a water content in the aquatic substance raw material to form an aquatic substance slurry to be processed; placing the aquatic substance slurry to be processed in a pressure container; introducing a compressed gas into the pressure container to enable the compressed gas and the water in the aquatic substance slurry to be processed to form an acidic fluid, and making the cell structure of the aquatic substance hydrolyzed and damaged by the acidic fluid; and performing a depressurizing step to separate the compressed gas.
    Type: Grant
    Filed: November 26, 2014
    Date of Patent: August 16, 2016
    Assignee: METAL INDUSTRIES RESEARCH & DEVELOPMENT CENTRE
    Inventors: Chun-Hung Hung, Chi-Hui Chen, Ching-Jui Tsai
  • Patent number: 9392793
    Abstract: Methods of treating contamination, particularly fungal contamination, in cultures of Haematococcus pluvialis with salt are described herein. The method comprises dosing the culture comprising with a concentration of salt based on the stage of the cells in the culturing process to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 9, 2015
    Date of Patent: July 19, 2016
    Assignee: HELIAE DEVELOPMENT LLC
    Inventors: Craig Erny, Laura Carney, Kristine Sorensen
  • Patent number: 9347034
    Abstract: Methods of treating lysis in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises detecting lysis in the culture and then dosing the culture with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 10, 2015
    Date of Patent: May 24, 2016
    Assignee: Heliae Development LLC
    Inventors: Laura Carney, Kristine Sorensen
  • Patent number: 9222065
    Abstract: The present invention relates to a method for photoculturing and harvesting microalgae, and more particularly to a method for photoculturing and harvesting microalgae, in which a light emitting diode (LED) is used as a light source to increase the efficiency with which microalgae are cultured, and the cultured microalgae are harvested using a natural polymer flocculant and air microbubbles so that the operating cost is reduced and the range of application of microalgae is expanded, and also a semi-continuous culture method is applied so that the maximum concentration of microalgae can be produced again within a short time, even though the amount of microalgae harvested is small.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: December 29, 2015
    Assignee: PUKYONG NATIONAL UNIVERSITY INDUSTRY-UNIVERSITY COOPERATION FOUNDATION
    Inventors: Tae yoon Lee, Jun-Heok Lim, Jea-Keun Lee
  • Patent number: 9200235
    Abstract: A method and system for processing algae involves the use of an ionic liquid-containing clarified cell lysate to lyse algae cells. The resulting crude cell lysate may be clarified and subsequently used to lyse algae cells. The process may be repeated a number of times before a clarified lysate is separated into lipid and aqueous phases for further processing and/or purification of desired products.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: December 1, 2015
    Assignee: Streamline Automation, LLC
    Inventors: Geoffrey Chew, Tabitha Boggs, H. Waite H. Dykes, Jr., Stephen J. Doherty
  • Patent number: 9200244
    Abstract: The invention relates to a method for cultivating photoautotrophic micro-organisms, comprising the following steps: provision of a photosynthesis layer, which contains a photosynthesis medium and photoautotrophic micro-organisms suspended therein, in a closed accommodation space having a gas-diffusion-inhibiting outer boundary, wherein the photosynthesis layer has an average thickness of no more than 10 mm; exposure of the photosynthesis layer without eddying or feeding through a gas in the photosynthesis medium while limiting the diffusive gas exchange of the accommodation space by the environment, wherein the supply of micro-organisms in the photosynthesis medium with hydrogen carbonate ions is maintained by storing hydrogen carbonate ions in the photosynthesis medium and/or providing a photosynthesis substrate in the form of carbon dioxide and hydrogen carbonate from a cell-free carbon storage medium outside the photosynthesis layer; and conducting a gas formed during photosynthesis out of the accommodatio
    Type: Grant
    Filed: November 16, 2012
    Date of Patent: December 1, 2015
    Assignee: Celldeg GbR
    Inventors: Rudolf Ehwald, Lars Bähr, Arne Wüstenberg, Joel Herve Soh
  • Patent number: 9180401
    Abstract: A method and system are described for on-board treatment of an exhaust stream containing CO2 emitted by a hydrocarbon-fueled internal combustion engine (ICE) used to power a vehicle in order to reduce the amount of CO2 discharged into the atmosphere which include: a.
    Type: Grant
    Filed: January 20, 2012
    Date of Patent: November 10, 2015
    Assignee: Saudi Arabian Oil Company
    Inventor: Esam Zaki Hamad
  • Patent number: 9132157
    Abstract: The present invention is an oral dosage composition for prevention and treatment of hepatic inflammation such as NASH. The present invention is an oral dosage composition for prevention or treatment of hepatic inflammation, contains the radical scavenging active spirulina powder effective in preventing, ameliorating, and curing NASH or the like.
    Type: Grant
    Filed: March 18, 2013
    Date of Patent: September 15, 2015
    Assignee: SPIRULINA BIO-LAB CO., LTD.
    Inventors: Yasumasa Kodo, Hiroshi Nishigaki
  • Patent number: 9096875
    Abstract: The present invention provides bicarbonate containing and/or bicarbonate-producing compositions and methods to induce lipid accumulation in an algae growth system, wherein the algae growth system is under light-dark cycling condition. By adding said compositions at a specific growth stage, said methods lead to much higher lipid accumulation and/or significantly reduced total time required for accumulating lipid in the algae growth system.
    Type: Grant
    Filed: September 26, 2011
    Date of Patent: August 4, 2015
    Assignee: Montana State University
    Inventors: Robert Gardner, Brent Peyton, Keith E. Cooksey
  • Patent number: 9085759
    Abstract: The invention relates to a process for the production of algal biomass with a high lipid content, comprising: (a) the production of inocula in order to effect phase (b), in photoreactors; (b) the massive cultivation of the algal biomass in open ponds, inoculated with phase (a); (c) a thickening phase of the algal biomass, effected blandly; (d) an induction phase of the lipid production, wherein modules are used consisting of photoreactors or open ponds; (e) a separation phase of the biomass with a high lipid content.
    Type: Grant
    Filed: December 3, 2008
    Date of Patent: July 21, 2015
    Assignee: ENI S.p.A.
    Inventors: Giacomo Rispoli, Emiliano Fioravanti, Renzo Bignazzi, Ezio Nicola D'Addario, Francesca De Ferra, Federico Capuano
  • Publication number: 20150147801
    Abstract: Provided is a method of culturing green algae which promotes the growth of the green algae which is in a state of being a green swarm cell by irradiating the green algae that accumulate astaxanthin with an artificial light, in which the green algae are grown in a liquid medium while maintaining a state in which the color of a culture solution of the green algae is green or brown by intermittently radiating a blue illumination light while continuously radiating a red illumination light.
    Type: Application
    Filed: February 4, 2014
    Publication date: May 28, 2015
    Applicant: SHOWA DENKO K.K.
    Inventors: Noriko Ohtake, Tadashi Yoneda
  • Patent number: 9040283
    Abstract: A photosynthetic reactor (1) suitable for cultivating photosynthetic microorganisms, notably algae, comprising at least one photosynthetic reaction pipe (2) at least one return pipe (3), at least one means (4) for circulating the liquid culture medium, at least one gas injection means (5) and at least one exhaust means (6) positioned in the high portion of the reactor (1), wherein the placement of the gas injection means (5) and/or the conformation of the reaction pipe (2) or of the return pipe (3) are designed so that the gas injected by the injection means (5) rises again to the exhaust means (6) by circulating in the reaction pipe (2), in a low-to-high flow direction, so that the injected gas and the liquid culture medium establish a gas/liquid two-phase flow in a substantially horizontal reaction section (23) of the reaction channel (2). The present invention finds application in the field of cultivating photosynthetic microorganisms and notably algae.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: May 26, 2015
    Assignee: MICROPHYT
    Inventor: Arnaud Muller-Feuga
  • Publication number: 20150140642
    Abstract: Provided is a method of culturing green algae which promotes the growth of the green algae which is in a state of being a green swarm cell by irradiating the green algae that accumulate astaxanthin with an artificial light, in which the green algae are grown in a liquid medium while maintaining a state in which the color of a culture solution of the green algae is green or brown by intermittently radiating a red illumination light while continuously radiating a blue illumination light.
    Type: Application
    Filed: February 4, 2014
    Publication date: May 21, 2015
    Applicant: SHOWA DENKO K.K.
    Inventors: Noriko Ohtake, Tadashi Yoneda
  • Patent number: 9034632
    Abstract: This disclosure provides compositions and methods for culturing microorganisms. The disclosure includes mixtures of different microorganisms, especially mixtures of cyanobacteria with fermentative microorganisms. For example, methods and compositions related to co-cultures of yeast and cyanobacteria are provided. Also provided are feedstocks derived from cyanobacteria as well as methods of making such feedstocks and methods of culturing microorganisms in such feedstocks.
    Type: Grant
    Filed: December 19, 2012
    Date of Patent: May 19, 2015
    Assignee: HelioBioSys, Inc.
    Inventors: Rocco Mancinelli, David Smernoff
  • Publication number: 20150132830
    Abstract: The invention relates to the field of microalgae culture and specifically relates to a trap-type carbon supplement device and carbon supplement method for cultivating microalgae in an open pond. The trap-type carbon supplement device for cultivating microalgae in an open pond, comprises a trap-type container, a partition plate and a gas distributor, wherein the gas distributor is positioned at the culture solution inlet of the trap-type carbon supplement device; the thickness of the trap-type carbon supplement device on the side of the culture solution inlet is 0.5-2 times of the depth of the culture solution in the open pond; the gap between the lower end of the partition plate and the bottom of the trap-type container is 0.5-2 times of the thickness of the trap- type carbon supplement device on the side of the culture solution inlet; the upper end of the partition plate is higher than the wall of the trap-type container; and the width of the partition plate is matched with the trap-type container.
    Type: Application
    Filed: May 7, 2012
    Publication date: May 14, 2015
    Applicant: Institute of Process Engineering, Chinese Academy of Sciences
    Inventors: Wei Cong, Zhongliang Sun, Ming Liu, Xia Wu, Dongmei Zhang, Shumei Wen
  • Publication number: 20150132829
    Abstract: The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.
    Type: Application
    Filed: January 23, 2015
    Publication date: May 14, 2015
    Applicant: SAPPHIRE ENERGY, INC.
    Inventors: Michael MENDEZ, Craig BEHNKE, Yan POON, Philip LEE
  • Patent number: 9029123
    Abstract: Methods and compositions are provided wherein microorganisms are used to alter the interface of hydrocarbons and hydrocarbon-coated surfaces to increase oil recovery, for improved bioremediation and/or to benefit pipeline maintenance.
    Type: Grant
    Filed: September 7, 2011
    Date of Patent: May 12, 2015
    Assignee: E I du Pont de Nemours and Company
    Inventors: Edwin R. Hendrickson, Abigail K. Luckring, Michael P. Perry
  • Publication number: 20150125923
    Abstract: The present invention generally relates to compositions and methods of delivering substances in a dry mode, wherein the compositions include a surface layer disposed on the outer surface of the composition that is permeable to carbon dioxide and oxygen. The compositions may be used to deliver microorganisms to remove contaminates, such as oil, chemical, waste, or sewage, from soil, water, or air. In other embodiments, the compositions can also be used for delivering liquid food, liquid food additives, liquid biotech agricultural ingredients, conventional liquid agricultural ingredients, liquid human wellness and dietary supplements, and liquid fragrances and beauty products.
    Type: Application
    Filed: October 5, 2011
    Publication date: May 7, 2015
    Applicant: DryLet LLC
    Inventors: Ramiro Trevino, Steven R. Ellis
  • Patent number: 9023625
    Abstract: The present invention comprises a method of producing algae derived oils comprising growing, stressing, and harvesting algae and then extracting total lipids from which specific oil fractions are purified.
    Type: Grant
    Filed: June 14, 2011
    Date of Patent: May 5, 2015
    Assignee: IO-Mega Holding Corporation
    Inventors: Raveendran Pottathil, Sanjay Kumar Amrutrao Deshmukh
  • Publication number: 20150118753
    Abstract: A method and system for achieving a gas-liquid mass transfer includes delivering into a compartment of a container a liquid, the liquid having an exposed top surface disposed within the compartment. A stream of a gas is passed over the top surface of the liquid so that the stream of gas produces turbulence on the top surface that is sufficient to achieve the gas-liquid mass transfer. In one embodiment the liquid is a culture that includes cells or microorganisms and the mass transfer functions to oxygenate the culture sufficient to sustain the cells or microorganisms.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 30, 2015
    Inventors: Christopher D. Brau, Nephi D. Jones, Benjamin R. Madsen, Michael E. Goodwin
  • Publication number: 20150118704
    Abstract: The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 30, 2015
    Inventors: Erika Geihe, Brian Trantow, Paul Wender, Joel M. Hyman, Bahram Parvin
  • Patent number: 9017985
    Abstract: The invention relates to the enrichment of photosynthetic microorganisms in organic selenium using selenohydroxy acid compounds, in particular 2-hydroxy-4-methylselenobutanoic acid, in D or L form, or an enantiomer, salt or ester or amide derivative of these compounds, and also to the use of the microorganisms thus enriched in animal or human nutrition, in cosmetics or in pharmacy.
    Type: Grant
    Filed: August 28, 2009
    Date of Patent: April 28, 2015
    Assignee: Metabolium
    Inventors: Bernard Kudla, Frédéric De Baene, Marc Lange
  • Patent number: 9018013
    Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
    Type: Grant
    Filed: August 13, 2012
    Date of Patent: April 28, 2015
    Assignee: Life Technologies Corporation
    Inventors: Farzad Haerizadeh, Todd Peterson, Wen Chen, Ewa Lis
  • Patent number: 9005918
    Abstract: The present disclosure provides an algae bioreactor and process. The algae bioreactor includes a container with an inlet and an outlet. An algae suspension present in the container interior moves from an inlet to an outlet along a flowpath. A light assembly, a liftwall, and a gas conduit are located in the container interior. The gas conduit extends along a length of the container and emits gas bubbles into the algae suspension. A diffuser is located on a bottom wall of the container, The flowpath, the liftwall, the gas conduit, and the diffuser produce an active flow of the algae from the container inlet to the container outlet. The container may be a deep-vessel container.
    Type: Grant
    Filed: December 15, 2011
    Date of Patent: April 14, 2015
    Assignee: DVO, Inc.
    Inventors: Stephen W. Dvorak, Douglas Van Ornum
  • Publication number: 20150093806
    Abstract: The present invention relates to a method for photoculturing and harvesting microalgae, and more particularly to a method for photoculturing and harvesting microalgae, in which a light emitting diode (LED) is used as a light source to increase the efficiency with which microalgae are cultured, and the cultured microalgae are harvested using a natural polymer flocculant and air microbubbles so that the operating cost is reduced and the range of application of microalgae is expanded, and also a semi-continuous culture method is applied so that the maximum concentration of microalgae can be produced again within a short time, even though the amount of microalgae harvested is small.
    Type: Application
    Filed: October 1, 2013
    Publication date: April 2, 2015
    Inventors: Tae yoon Lee, Jun-Heok Lim, Jea-Keun Lee
  • Patent number: 8993314
    Abstract: A system for culturing photosynthesizing microorganisms includes a source of a gaseous fluid a mixer that creates micron bubbles within an aqueous medium using the gaseous fluid. The mixing chamber holds the aqueous medium including the micron bubbles before the micron bubbles and aqueous medium are mixed with a culture of photosynthesizing microorganism in a reaction chamber.
    Type: Grant
    Filed: July 28, 2007
    Date of Patent: March 31, 2015
    Assignee: Ennesys SAS
    Inventors: Nicholas Eckelberry, T. Riggs Eckelberry
  • Publication number: 20150087048
    Abstract: A method and apparatus for processing microorganisms is provided. The method comprises mixing microorganisms with a working fluid to form an working fluid slurry, and injecting a transport fluid through a transport fluid nozzle into the working fluid slurry in order to disrupt the cellular structure of the microorganisms.
    Type: Application
    Filed: June 22, 2012
    Publication date: March 26, 2015
    Inventors: Marcus Brian Mayhall Fenton, Olga Koroleva, Michelle Gina Elizabeth Gothard, Christopher Drake
  • Patent number: 8980618
    Abstract: Methods are provided for separating algae from an aqueous environment. An aqueous feed containing algae can be aged for a period of at least about 90 hours without purging or other aeration. The aging of the aqueous feed can result in formation of aggregated algae, which can then be filtered under pressure. Due to the larger size of the algae aggregates, the pressurized filtration can reduce/minimize the tendency to clog the filter, as the algae aggregates can generally be too large to fit within the pores of the filter. The filter can preferably be located at the bottom of the vessel holding the aqueous feed. This can assist in allowing the algae to remain in a cake above the filter, as opposed to having the algae dissolve back into the aqueous feed.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: March 17, 2015
    Assignee: Exxonmobil Research and Engineering Company
    Inventor: Ramesh Varadaraj
  • Publication number: 20150072400
    Abstract: Methods of cultivating autotrophic microorganisms, particularly microalgae or diatoms, in a bioreactor by entraining a culture of the microorganisms in a tenuous, gelated, thixotrophic carrier medium having nutrients therefor and moving the medium along a passage at a sufficiently slow speed to enable laminar flow which in cross section is closed and which has transparent walls through which the culture is irradiated to enable photosynthesis. The method includes effecting convective turnover of the culture and medium as they flow along the passage by differentially heating the medium laterally relative to the flow direction so as to produce a generally helical flow of the culture and medium. Also described are processing methods, both physical and chemical, performed underground e.g.
    Type: Application
    Filed: May 5, 2014
    Publication date: March 12, 2015
    Inventor: William Severn Clarke