Chlorella Patents (Class 435/257.3)
  • Patent number: 10584344
    Abstract: Microbes can be genetically modified to express biomolecules that are beneficial to mammals and/or to reduce, or eliminate, expression of harmful virulence factors. The growth and viability of such genetically modified microbes can optionally be controlled by inducible promoters that regulate the expression of proteins that are essential to their growth and survival. Compositions comprising such genetically modified microbes as well as methods of making and using the same are disclosed herein.
    Type: Grant
    Filed: June 17, 2015
    Date of Patent: March 10, 2020
    Assignee: CROWN LABORATORIES, INC.
    Inventors: Thomas Hitchcock, Mun Su Rhee
  • Patent number: 9580730
    Abstract: New strains of microalgae belonging to the Nitzschia genus, allow high-yield production of lipids, in particular of docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA) and/or carotenoids, in particular fucoxanthin, in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 28, 2017
    Assignee: FERMENTALG
    Inventors: Khadidja Romari, Francois Godart, Pierre Calleja
  • Patent number: 9556468
    Abstract: Novel strains of microalgae belonging to the Scenedesmus genus enable the production of lipids, in particular lutein, in mixotrophic mode, as well as a method for selecting and culturing the strains using a variable and/or discontinuous supply of light, in particular in the form of flashes.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: January 31, 2017
    Assignee: FERMENTALG
    Inventors: Khadidja Romari, Francois Godart, Pierre Calleja
  • Patent number: 9506100
    Abstract: New strains of protists belonging to the Schizochytrium genus, allow high-yield production of lipids and carotenoids, in particular of astaxanthin and docosahexaenoic acid (DHA), in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: November 29, 2016
    Assignee: FERMENTALG
    Inventors: Khadidja Romari, Adeline Le Monnier, Cyril Rols, Cindy Merlet, Julien Pagliardini, Pierre Calleja, Claude Gudin
  • Publication number: 20150132830
    Abstract: The invention relates to the field of microalgae culture and specifically relates to a trap-type carbon supplement device and carbon supplement method for cultivating microalgae in an open pond. The trap-type carbon supplement device for cultivating microalgae in an open pond, comprises a trap-type container, a partition plate and a gas distributor, wherein the gas distributor is positioned at the culture solution inlet of the trap-type carbon supplement device; the thickness of the trap-type carbon supplement device on the side of the culture solution inlet is 0.5-2 times of the depth of the culture solution in the open pond; the gap between the lower end of the partition plate and the bottom of the trap-type container is 0.5-2 times of the thickness of the trap- type carbon supplement device on the side of the culture solution inlet; the upper end of the partition plate is higher than the wall of the trap-type container; and the width of the partition plate is matched with the trap-type container.
    Type: Application
    Filed: May 7, 2012
    Publication date: May 14, 2015
    Applicant: Institute of Process Engineering, Chinese Academy of Sciences
    Inventors: Wei Cong, Zhongliang Sun, Ming Liu, Xia Wu, Dongmei Zhang, Shumei Wen
  • Publication number: 20150118704
    Abstract: The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 30, 2015
    Inventors: Erika Geihe, Brian Trantow, Paul Wender, Joel M. Hyman, Bahram Parvin
  • Publication number: 20150118735
    Abstract: Methods of culturing microorganisms in non-axenic mixotrophic conditions are disclosed. A method of culturing microalgae mixotrophically and controlling bacterial contamination with an acetic acid/pH auxostat system is specifically described. Methods of culturing microalgae mixotrophically with an increased productivity through an increase in oxygen transfer to the culture, and controlling bacterial contamination with an oxidative agent are also described.
    Type: Application
    Filed: December 30, 2014
    Publication date: April 30, 2015
    Inventors: Eneko GANUZA, Anna Lee TONKOVICH, Jason D. LICAMELE
  • Patent number: 9018013
    Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
    Type: Grant
    Filed: August 13, 2012
    Date of Patent: April 28, 2015
    Assignee: Life Technologies Corporation
    Inventors: Farzad Haerizadeh, Todd Peterson, Wen Chen, Ewa Lis
  • Patent number: 9017985
    Abstract: The invention relates to the enrichment of photosynthetic microorganisms in organic selenium using selenohydroxy acid compounds, in particular 2-hydroxy-4-methylselenobutanoic acid, in D or L form, or an enantiomer, salt or ester or amide derivative of these compounds, and also to the use of the microorganisms thus enriched in animal or human nutrition, in cosmetics or in pharmacy.
    Type: Grant
    Filed: August 28, 2009
    Date of Patent: April 28, 2015
    Assignee: Metabolium
    Inventors: Bernard Kudla, Frédéric De Baene, Marc Lange
  • Publication number: 20150004673
    Abstract: The present invention relates to natural crude salt formulations arid their cost-effective preparation. In particular, the invention discloses the fractionation of seawater into three fractions which can thereafter be suitably combined to reconstitute seawater in its pristine form for its various applications such as growth of marine microbes, flora and fauna in a marine environment, especially where such environments have to be created in locations far removed from the sea and/or where there is a requirement to modify the composition of seawater to better its performance. The fractions can also be blended appropriately to exclude the major constituent, namely sodium chloride, and thereby be useful for mineralization/re-mineralization of waters which are deficient in mineral nutrients such as calcium, magnesium, potassium, sulphate and bicarbonate including rain water and desalinated waters obtained through thermal/RO desalination.
    Type: Application
    Filed: December 27, 2012
    Publication date: January 1, 2015
    Inventors: Pushpito Kumar Ghosh, Sumesh Chandra Upadhyay, Sandhya Chandrikaprasad Mishra, Vadakke Puthoor Mohandas, Divesh Narayan Srivastava, Vinod Kumar Shahi, Rahul Jasvantrai Sanghavi, Sreekumaran Thampy, Babulal Surabhai Makwana, Imran Pancha, Ruma Pal, Ramkrishna Sen
  • Publication number: 20150004674
    Abstract: Provided is a method for removing radionuclides using microalgae and A composition for removing radionuclides. The method includes bring the radionuclides into contact with microalgae, and thus a method for easily removing radionuclides in eco-friendly manner is provided, and also a method for removing radionuclides with high efficiency is provided. A composition for removing radionuclides includes microalgae.
    Type: Application
    Filed: May 22, 2014
    Publication date: January 1, 2015
    Applicant: SOGANG UNIVERSITY RESEARCH FOUNDATION
    Inventors: Seung-Yop LEE, Kwang-Hwan JUNG, Sang-Hyo LEE, Keon Ah LEE, Ju Eun LEE, Seung Yeop LEE
  • Publication number: 20140318000
    Abstract: The invention relates to the field of culturing algae and use of algae-derived products. The invention also relates to the reduction of CO2 emission. More in particular the invention relates to combining the capture and conversion of CO2 from flue gas or biogas and the growth of algae. The invention provides a method for advantageously combining reduction of CO2 emission with algal growth.
    Type: Application
    Filed: August 10, 2012
    Publication date: October 30, 2014
    Applicant: Nederlandse Organisatie voor toegepast-natuurwetenschappelijk onderzoek TNO
    Inventors: Earl Lawrence Vincent Goetheer, Leo Jacques Pierre Van Den Broeke, Judith Jahn, Willempje Antonie Patricia Van Den Bos, Cornelis Petrus Marcus Roelands
  • Publication number: 20140315267
    Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.
    Type: Application
    Filed: May 13, 2014
    Publication date: October 23, 2014
    Applicant: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, Janice Wee, George Rudenko, Jeffrey L. Moseley, Walt Rakitsky
  • Publication number: 20140295448
    Abstract: The invention provides method and compositions to minimize the chlorophyll antenna size of photosynthesis by decreasing TLA2 gene expression, thereby improving solar conversion efficiencies and photosynthetic productivity in green microalgae, under bright sunlight conditions.
    Type: Application
    Filed: October 24, 2012
    Publication date: October 2, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Anastasios Melis, Henning Kirst
  • Patent number: 8802422
    Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.
    Type: Grant
    Filed: June 2, 2008
    Date of Patent: August 12, 2014
    Assignee: Solazyme, Inc.
    Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
  • Publication number: 20140220638
    Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher Pmax per unit chlorophyll with little to no reduction in Pmax per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.
    Type: Application
    Filed: December 6, 2013
    Publication date: August 7, 2014
    Applicant: Synthetic Genomics, Inc.
    Inventors: Shaun Bailey, Jay McCarren, Soyan Leung Lieberman, Jonathan E. Meuser, Anna E. Romano, Daniel Yee, Leah Soriaga, Robert C. Brown, Joseph C. Weissman, Roger C. Prince, Robert D. Nielsen, Ariel S. Schwartz
  • Patent number: 8790914
    Abstract: The invention provides methods of cultivating oil-bearing microbes using cellulosic material. Also provided are microorganisms that manufacture non-alcohol-based fuels and fuel feedstocks through a process of converting cellulosic materials into oils. Also provided are compositions comprising depolymerized cellulosic materials and oil-bearing microbes. Some methods of microbial fermentation are provided that comprise combining depolymerized cellulosic materials with other non-cellulosic feedstocks to enhance the economics of renewable fuel manufacturing. Particular advantages of the processes provided herein include production of oils rather than alcohols through cellulosic processes. Additional advantages include methods for manufacturing high nutrition oils from non-edible feedstocks such as wood chips, switchgrass, and bagasse.
    Type: Grant
    Filed: June 2, 2008
    Date of Patent: July 29, 2014
    Assignee: Solazyme, Inc.
    Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
  • Publication number: 20140186842
    Abstract: The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.
    Type: Application
    Filed: December 5, 2013
    Publication date: July 3, 2014
    Applicant: Synthetic Genomics, Inc.
    Inventors: Rekha Seshadri, Ariel S. Schwartz, Leah Soriaga, Robert C. Brown
  • Patent number: 8759615
    Abstract: Exemplary methods include a method for transforming an algal cell by preparing a transformation construct, preparing a particle for bombarding the algal cell, adhering the transformation construct to the particle, bombarding the algal cell with the particle, and growing the algal cell into a colony. The transformation construct is replicated within a nuclear genome of the algal cell and the growing of the algal cell is in a nutrient medium. Another exemplary method may include a method for genetically modifying an algal cell, by adding nucleic acid to the algal cell while the algal cell is suspended in a solution of low conductivity, introducing the nucleic acid into the algal cell by application of an electrical pulse resulting in a transformed algal cell, and selecting a colony that includes the transformed algal cell.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: June 24, 2014
    Assignee: Aurora Algae, Inc.
    Inventors: Bertrand Vick, Oliver Kilian
  • Publication number: 20140170733
    Abstract: As a simple and convenient method for promoting the proliferation of algae, a method for cultivating the algae while conducting a procedure S1 for irradiating a red illuminative light to the algae and a procedure S2 for irradiating a blue illuminative light to the algae separately and independently of each other within a certain time period is provided.
    Type: Application
    Filed: March 27, 2012
    Publication date: June 19, 2014
    Applicants: YAMAGUCHI UNIVERSITY, SHOWA DENKO K.K.
    Inventors: Masayoshi Shigyo, Hiroshi Suzuki, Naoki Yamauchi, Hironori Ara, Akihiro Shimokawa, Misato Matsumoto, Yuki Tonooka
  • Patent number: 8741628
    Abstract: An energy efficient process for the preparation of marine microalgae Chlorella fatty acid methyl ester (CME) from hydrolysate of deoiled cake of Jatropha (JOCH) and crude glycerol co-product stream (GL7 and GL8) along with seawater diluted with tap water (1:2). A small part of the crude glycerol layer in case of JME is processed to recover glycerol for glycerol washing and the otherwise problematic still bottom is utilized for microbial synthesis of PHAs and the rest is utilized for Microalgal conversion of JME byproducts into CME. The remaining part of the methanol-depleted glycerol layer is utilized, along with hydrolysate of the Jatropha deoiled cake (JOCH), for single-stage Microalgal production of lipids by a marine Microalgal isolate (Chlorella sp.) without the need for any other nutrients. Waste streams from the microalgal processes can be discharged directly into agricultural fields as biofertilizer or recycled back in the mass cultivation.
    Type: Grant
    Filed: September 22, 2011
    Date of Patent: June 3, 2014
    Assignee: Council of Scientific and Industrial Research
    Inventors: Pushpito Kumar Ghosh, Sandhya Chandrika Prasad Mishra, Mahesh Ramniklal Gandhi, Sumesh Chandra Upadhyay, Sanjiv Kumar Mishra, Imran Pancha, Anupama Vijaykumar Shrivastav, Deepti Jain, Bhumi Shethia, Subama Maiti, Krushnadevsinh Sukhdevsingh Zala
  • Patent number: 8735140
    Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.
    Type: Grant
    Filed: January 30, 2012
    Date of Patent: May 27, 2014
    Assignee: Kuehnle AgroSystems, Inc.
    Inventor: Adelheid R. Kuehnle
  • Patent number: 8722389
    Abstract: A system and method for culturing algae are presented. The system and method utilize a fog of growth medium that is delivered to an algal mat generator along with a stream of CO2 to promote growth of algal cells contained in the generator.
    Type: Grant
    Filed: April 19, 2011
    Date of Patent: May 13, 2014
    Assignee: U.S. Department of Energy
    Inventors: Keshav C. Das, Benjamin R. Cannon, Ashish Bhatnagar, Senthil Chinnasamy
  • Patent number: 8697402
    Abstract: The invention provides methods of manufacturing biodiesel and other oil-based compounds using glycerol and combinations of glycerol and other feedstocks as an energy source in fermentation of oil-bearing microorganisms. Methods disclosed herein include processes for manufacturing high nutrition edible oils from non-food feedstock materials such as waste products from industrial waste transesterification processes. Also included are methods of increasing oil yields by temporally separating glycerol and other feedstocks during cultivation processes. Also provided herein are oil-bearing microbes containing exogenous oil production genes and methods of cultivating such microbes on glycerol and other feedstocks.
    Type: Grant
    Filed: August 19, 2008
    Date of Patent: April 15, 2014
    Assignee: Solazyme, Inc.
    Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
  • Publication number: 20140073011
    Abstract: Method for the production of aqueous nutrient source for macro and micro algae aquaculture farming and nutrient source thus formed. Phosphorous containing rocks are dissolved in at least one mineral acid to yield a solution containing a blend of ions of the minerals included in the rocks and in the acid in a form digestible by the algae and adding carbon in the form of CO2, carbonic acid, or carbonate salts. The invention is also directed to a method for feeding an algae aquaculture farm with such nutrient source.
    Type: Application
    Filed: October 11, 2013
    Publication date: March 13, 2014
    Inventors: Oyvind BRANDVOLL, Harald JOHANSEN, Ingo MACHENBACH, Arne RAAHEIM
  • Publication number: 20130309757
    Abstract: The present invention relates to a method and apparatus for producing cells without injury and fat-soluble materials by from cell culturing in an inexpensive and highly efficient manner. The apparatus according to the present invention comprises a culturing device 10, a solvent device 20, a mixing device 30, a separation device 40, a fractionation device 50, a cell accommodation device 60, and a fat-soluble material solvent accommodation device 70.
    Type: Application
    Filed: December 16, 2011
    Publication date: November 21, 2013
    Inventor: Sung-Chun Kim
  • Patent number: 8586344
    Abstract: The invention relates to a tubular photobioreactor that has a core structure in the shape of a truncated cone and one or more transparent or translucent tubes which are helically wound around the outer surface and/or inner surface of the core structure. The tubular photobioreactor is characterized in that the transparent or translucent tube has at least two chambers, through at least one of which the cultivation medium flows and through at least one of which a heat transfer medium flows.
    Type: Grant
    Filed: October 19, 2010
    Date of Patent: November 19, 2013
    Assignee: Wacker Chemie AG
    Inventors: Christoph Mueller-Rees, Rupert Pfaller, Christian Walter, Fritz Cotta
  • Patent number: 8557249
    Abstract: The invention provides cosmetic compositions comprising microalgal biomass, whole microalgal cells, and/or microalgal oil in combination with one or more other cosmetic ingredients, and methods of making such compositions. In preferred embodiments, the microalgal components of the cosmetic compositions are derived from microalgal cultures grown heterotrophically and which comprise at least 10% oil by dry weight.
    Type: Grant
    Filed: November 9, 2009
    Date of Patent: October 15, 2013
    Assignee: Solazyme, Inc.
    Inventors: Geoffrey Brooks, Scott Franklin
  • Publication number: 20130244310
    Abstract: A system and method of growing and harvesting algae provided whereby the system encompasses incubation tanks, internal lighting, chilled air diffusers, and an inline incubation tank for continuous batch processing. A centrifuge separates algae from growth media, and the media is processed through a series of reclamation steps so that cleaned water is reused for fresh media.
    Type: Application
    Filed: October 9, 2012
    Publication date: September 19, 2013
    Inventor: Geronimos Dimitrelos
  • Patent number: 8497116
    Abstract: The invention provides methods of manufacturing oils and oil-based products such as transportation fuels, industrial chemicals, lubricants and plastics using sugar cane, sugar cane juice, corn steep liquor, corn starch or depolymerized cellulosic material as a feedstock for bioproduction processes. Oils are manufactured using microalgae expressing sucrose invertase, which allows the microalgae to produce oil using sucrose-based feedstocks, along or in combination with other feedstocks.
    Type: Grant
    Filed: December 3, 2010
    Date of Patent: July 30, 2013
    Assignee: Solazyme, Inc.
    Inventors: Donald E. Trimbur, Chung-Soon Im, Harrison F. Dillon, Anthony G. Day, Scott Franklin, Anna Coragliotti
  • Patent number: 8445257
    Abstract: The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses.
    Type: Grant
    Filed: December 28, 2011
    Date of Patent: May 21, 2013
    Assignee: Synthetic Genomics, Inc.
    Inventors: Paul Gordon Roessler, Bo Liu, Jessica Roxane Kristof
  • Publication number: 20130102055
    Abstract: A continuous process for efficiently harvesting microalgae from aqueous systems is described herein. The method and apparatus of the present invention allows continuous harvesting of algae from a variety of source waters including saltwater, brackish water, fresh water, and treated wastewater. High concentration factors are achievable and the system produces a deflocculated product that is readily processed for biofuel or pharmaceutical applications. The process of the present invention does not add contaminants that can limit the downstream usage possibilities for the algae concentrate produced. The effluent water from the process is suitable for conventional discharge or recycling to the growth system. The process of the present invention is inexpensive, scalable, and generates useful effluent water and algae concentrate as products.
    Type: Application
    Filed: October 20, 2012
    Publication date: April 25, 2013
    Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
    Inventor: BOARD OF REGENTS, THE UNIVERSITY OF TE
  • Publication number: 20130065313
    Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.
    Type: Application
    Filed: August 13, 2012
    Publication date: March 14, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Farzad Haerizadeh, Todd Peterson, Wen Chen, Ewa Lis
  • Patent number: 8362318
    Abstract: The present invention is related to biosynthetic oil compositions and methods of making thereof. In some embodiments, the invention relates to the use of endogenous enzymes in plants capable of synthesizing oil. In preferred embodiments, said plants are algae. In further embodiments, said algae are from the family Chlamydomonas, Nannochloropsis, Dunaliella, Chlorella and Scenedesmus. In still further embodiments, said endogenous enzymes are diacylglycerol acyltransferases.
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: January 29, 2013
    Assignee: Board of Trustees of Michigan State University
    Inventors: Christoph Benning, Rachel Miller, Eric R. Moellering
  • Patent number: 8349335
    Abstract: The present invention relates to a method for preparing chlorophyll-a and chlorin e6. This invention extracts chlorophyll-a by use of undisrupted chlorella cells themselves, thereby preparing chlorin e6 from the chlorophyll-a extract. The high contents of chlorophyll-a may be obtained by the pretreatment procedure of chlorella cells themselves selected in this invention. The present method is performed according to relatively simple procedures, and is suitable in the mass production of chlorin e6.
    Type: Grant
    Filed: May 3, 2007
    Date of Patent: January 8, 2013
    Assignee: Gwangju Institute of Science and Technology
    Inventors: Yong Chul Kim, Zee Yong Park, Hyo Jun Kim
  • Publication number: 20120329138
    Abstract: A process for separation of a mixture containing a microbial substance and a liquid using deformable filter is provided.
    Type: Application
    Filed: December 19, 2011
    Publication date: December 27, 2012
    Applicant: SHELL OIL COMPANY
    Inventors: Henry Van Kaathoven, Johannes Pieter Haan, Wilhelmus Maria Bond, Johannes Leendert Willem Cornelis Den Boestert
  • Publication number: 20120323029
    Abstract: Methods, biological oils, biofuels, units, and/or organisms directed to use in compression engines. A method of producing biological oils includes producing an organism and having the organism consume a feedstock. The organism includes a lipid containing fatty acids. The organism meets or exceeds at least two metrics. The metrics include: A) a cell density of at least about 115 grams per liter; B) a fatty acid content of at least about 49 percent on a dry mass basis; C) a fatty acid productivity of at least about 15 grams per liter per day; D) a fatty acid yield of at least about 0.175 grams of fatty acids produced per grams of the feedstock consumed; E) a 24 hour peak fatty acid productivity of at least about 30 grams per liter per day; F) an extraction efficiency on a percent of total fatty acid content basis of at least about 50 percent; and/or G) yield of fatty acids on oxygen of more than about 0.4 as grams of fatty acids produced per gram of oxygen consumed basis.
    Type: Application
    Filed: March 8, 2011
    Publication date: December 20, 2012
    Applicant: BP Biofuels IK Limited
    Inventors: Joseph W. Pfeifer, III, Paul W. Behrens, Michael A. Johnson, Jon M. Hansen, Kirk E. Apt, William R. Barclay, David Christian Martin, Jacob Borden
  • Patent number: 8329449
    Abstract: A reverse phase extraction method for the recovery of triglycerides from aqueous slurries of algae is described herein. The present invention discloses the use of immobilized anion exchange and lipid binding resins. As the algae flows past the resin, triglycerides adhere while the bulk of the algae flows through. The lipids, useful for generating biofuels are then are eluted off the resin. The method of the present invention does not require prior drying of the algae, is inexpensive, and does not destroy the algal biomass which can be used for other purposes.
    Type: Grant
    Filed: October 13, 2010
    Date of Patent: December 11, 2012
    Assignee: Board of Regents, The University of Texas System
    Inventors: Martin Poenie, Jessica Jones, James Beach
  • Patent number: 8288150
    Abstract: A method for breaking the cell walls of microalgae includes cultivating microbes in a cultivating liquid, adding microalgae into the cultivating liquid to mix with the microbes, releasing a hydrolysis ferment from the microbes, hydrolyzing cell walls of the microalgae by the hydrolysis ferment of the microbes to decompose the cell walls of the microalgae into saccharide, and removing the microalgae from the cultivating liquid. Thus, the microbes release the hydrolysis ferment after the microalgae touch the microbes so as to hydrolyze and decompose the cell walls of the microalgae in a moderate manner without breaking the contents of the microalgae so that the contents of the microalgae can be released, absorbed and used completely.
    Type: Grant
    Filed: July 9, 2010
    Date of Patent: October 16, 2012
    Assignee: Analytica Bioenergy, Inc.
    Inventors: Jiunn-Jye Chuu, Shun-Lai Li, Hsiao-Hui Hsieh, Chia-Hui Cheng
  • Publication number: 20120252105
    Abstract: Cultivators and methods of cultivating microorganisms are provided. In some examples, a cultivator may include a retaining wall defining a cavity for retaining liquid and may also include a horizontally orientated frame at least partially positioned within the cavity and having media for supporting microorganisms. In other examples, the horizontal frame may be partially submerged in the liquid retained in the cavity and rotatable to selectively submerge and unsubmerge portions of the frame and media.
    Type: Application
    Filed: September 22, 2011
    Publication date: October 4, 2012
    Applicant: BIOPROCESSH20 LLC
    Inventors: Toby D. Ahrens, Shawn R. Kitchner, John W. Haley, III
  • Publication number: 20120225472
    Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.
    Type: Application
    Filed: January 30, 2012
    Publication date: September 6, 2012
    Applicant: KUEHNLE AGROSYSTEMS, INC.
    Inventor: ADELHEID R. KUEHNLE
  • Publication number: 20120220021
    Abstract: Disclosed herein are transformed non-vascular photosynthetic organisms that are herbicide resistant, nucleotides and vectors useful in conducting such transformations, and transformed strains produced by such transformations.
    Type: Application
    Filed: September 15, 2010
    Publication date: August 30, 2012
    Applicant: SAPPHIRE ENERGY ,INC.
    Inventors: Tanya Joy Shin, Yan Poon, Melisa C. Low, Shane Edward Hopkins, Alvin Aram Cho
  • Publication number: 20120214198
    Abstract: The present invention relates to bioprocess engineering methods in which aqueous phases of anaerobic biologically treated organic suspensions are supplied to algal cultures as media components. The present invention also relates to the use of aqueous phases of anaerobic biologically treated organic suspensions as media components of algal cultures. Additionally, the present invention relates to the use of aqueous phases of anaerobic biologically treated organic suspensions to improve the growth conditions of algae in photobioreactors. Furthermore, the present invention relates to the use of algae for the treatment of aqueous phases of anaerobic biologically treated organic suspensions, in particular of anaerobic biologically treated sewage filtrate. The present invention also relates to bioprocess engineering devices (100) comprising a bioreactor (1), in particular a fermentation tower and a photobioreactor (3).
    Type: Application
    Filed: October 15, 2010
    Publication date: August 23, 2012
    Applicant: FRAUNHOFER-GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
    Inventor: Walter Trösch
  • Publication number: 20120202290
    Abstract: The invention relates to a tubular photobioreactor that has a core structure in the shape of a truncated cone and one or more transparent or translucent tubes which are helically wound around the outer surface and/or inner surface of the core structure. Said tubular photobioreactor is characterized in that the transparent or translucent tube has at least two chambers, through at least one of which the cultivation medium flows and through at least one of which a heat transfer medium flows.
    Type: Application
    Filed: October 19, 2010
    Publication date: August 9, 2012
    Applicant: WACKER CHEMIE AG
    Inventors: Christoph Mueller-Rees, Rupert Pfaller, Christian Walter, Fritz Cotta
  • Patent number: 8227237
    Abstract: The present disclosure identifies pathways and mechanisms to confer production of carbon-based products of interest such as ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof in photoautotrophic organisms such that these organisms efficiently convert carbon dioxide and light into carbon-based products of interest, and in particular the use of such organisms for the commercial production of ethanol, ethylene, chemicals, polymers, n-alkanes, isoprenoids, pharmaceutical products or intermediates thereof.
    Type: Grant
    Filed: June 30, 2011
    Date of Patent: July 24, 2012
    Assignee: Joule Unlimited Technologies, Inc.
    Inventors: Nikos B. Reppas, Martha Sholl, Sriram Kosuri, Brian D. Green, Christian P. Ridley, Frank A. Skraly, Dan E. Robertson, David A. Berry, Noubar B. Afeyan
  • Patent number: 8216825
    Abstract: Methods and compositions for increasing the productivity of algae cultures by controlling grazers are described herein.
    Type: Grant
    Filed: March 7, 2011
    Date of Patent: July 10, 2012
    Inventor: Alex Yuan
  • Publication number: 20120171753
    Abstract: System and method are provided for treating materials in a liquid in a pool having a bottom, using at least one of a stirring device, a brushing device and a wiping device, and an automatic moving arrangement configured for moving any of these devices in the pool. The stirring device comprises at least one wing having a longitudinal axis and at least two bottom contacting elements spaced from each other along the longitudinal axis of the wing so that, when the wing is in such close proximity to the bottom of the pool that the contacting elements contact its bottom, the contact of the wing with the bottom is prevented by the contacting elements.
    Type: Application
    Filed: September 6, 2011
    Publication date: July 5, 2012
    Applicant: SEAMBIOTIC LTD.
    Inventor: Itai IVRY
  • Patent number: 8206984
    Abstract: Disclosed are nucleic acid and amino acid sequences for acetolactate synthase, acetolactate synthase regulatory regions, ?-tubulin promoter, a promoter from a Thraustochytriales polyketide synthase (PKS) system, and fatty acid desaturase promoter, each from a Thraustochytriales microorganism. Also disclosed are recombinant vectors useful for transformation of Thraustochytriales microorganisms, as well as a method of transformation of Thraustochytriales microorganisms. The recombinant nucleic acid molecules of the present invention can be used for the expression of foreign nucleic acids in a Thraustochytriales microorganism as well as for the deletion, mutation, or inactivation of genes in Thraustochytriales microorganisms.
    Type: Grant
    Filed: December 22, 2004
    Date of Patent: June 26, 2012
    Assignee: Merck Biosciences Corporation
    Inventors: Paul G. Roessler, T. Dave Matthews, Tom M. Ramseier, James G. Metz
  • Publication number: 20120088279
    Abstract: Methods for increasing the levels of lipids in oleaginous algae are described. Lipid levels in algae can be increased by stress, such as nutrient stress, after which the lipid can be harvested from the algae using a non-destructive extraction process. The stress may be provided in a periodic or “pulsed” fashion. Lipid levels in oleaginous algae can also be increased using simulated stress by treating the algae with a chemical inhibitor or by using recombinant technology to insert a sequence expressing a protein such as a nitrate reductase inhibitor that is expressed when a stressed state is desired. A method for maintaining the temperature and water levels of algae ponds using buoyant spheres is also described.
    Type: Application
    Filed: May 11, 2010
    Publication date: April 12, 2012
    Applicant: Phycal, Inc.
    Inventors: Andrew Keith Swanson, Bradley Lynn Postler, Richard T. Sayre
  • Publication number: 20120077250
    Abstract: This invention provides methods for growing and maintaining photosynthetic microorganisms such as algae and cyanobacteria. More specifically, the invention provides methods of cultivation of photosynthetic microorganisms on bacterial cellulose.
    Type: Application
    Filed: September 23, 2010
    Publication date: March 29, 2012
    Applicant: UT-BATTELLE, LLC
    Inventor: Barbara R. Evans