Euglena Patents (Class 435/257.4)
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Patent number: 10766799Abstract: A method for improving the quality of pond water used in aquaculture applications by adding to the pond water active bacteria that are preferably germinated from spores on site using a nutrient-germinant composition and an incubation method for increased spore germination efficiency, in combination with a nitrification enhancement agent such as calcium carbonate or calcified seaweed, and an optional reaction surface area modifier such as calcified seaweed or plastic or metal particles or fragments. The nutrient-germinant composition comprises L-amino acids, D-glucose and/or D-fructose, a phosphate buffer, an industrial preservative, and may include bacteria spores (preferably of one or more Bacillus species) or they may be separately combined for germination. The incubation method comprises heating a nutrient germinant composition and bacteria spores, to a temperature range of 35° C. to 60° C.Type: GrantFiled: February 28, 2018Date of Patent: September 8, 2020Assignee: NCH CorporationInventors: Charles Greenwald, Gabriel F. K. Everett, Judy Pruitt, Amanda Rosmarin, Jordan Church, Daniel Aberle, George Aboagye, Skylar Rae White, Haibo Cao, Christopher Zetena, Kelly Gillespie
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Patent number: 9790461Abstract: A culture method for microalgae that cultures unicellular green microalgae belonging to the genus Coccomyxa and groups of organisms closely related thereto, or the Watanabea clade, in an open outdoor culture system using broth having a pH of 4 or lower. A culture method for microalgae that cultures microalgae of the genus Coccomyxa and groups of organisms closely related thereto, or Pseudococcomyxa, in an open outdoor culture system using broth having a pH of 4 or lower containing ammonia nitrogen.Type: GrantFiled: December 4, 2013Date of Patent: October 17, 2017Assignees: DENSO CORPORATION, Kyoto University, Chuo UniversityInventors: Minoru Kurata, Hiroaki Fukuda, Norihide Kurano, Hideaki Miyashita, Shigeaki Harayama
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Patent number: 9018013Abstract: The present invention provides compositions and methods for the genetic manipulation of Algal cells. The compositions and methods allow enhanced transfer of genetic material into Algal cells and the cloning and selection of genetically modified cells. Expression of proteins encoded by the genetic material will be enhanced by the methods and compositions of the invention.Type: GrantFiled: August 13, 2012Date of Patent: April 28, 2015Assignee: Life Technologies CorporationInventors: Farzad Haerizadeh, Todd Peterson, Wen Chen, Ewa Lis
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Publication number: 20150044738Abstract: New strains of microalgae belonging to the Euglena genus, allow production of lipids, in particular of EPA and/or ARA, in mixotrophic mode, and a method for selecting and culturing such strains, using a variable and/or discontinuous light source, in particular a flashing light.Type: ApplicationFiled: March 15, 2013Publication date: February 12, 2015Applicant: FERMENTALGInventors: Khadidja Romari, Pierre Calleja
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Patent number: 8951773Abstract: The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S1) of culturing in a culture solution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process (S2) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process (S3) of extracting the oils and fats out of the cells of the photosynthetic microorganisms, and a reforming process (S4) to reform the extracted oils and fats.Type: GrantFiled: May 25, 2011Date of Patent: February 10, 2015Assignees: Hitachi, Ltd., JX Nippon Oil & Energy Corporation, euglena Co., Ltd.Inventors: Hiroaki Kato, Ko Yamashita, Yukio Fukushima, Ken Amano, Takashi Kaneko, Iwao Ueda, Nobuo Aoki, Kengo Suzuki, Ryo Arashida, Ryohei Nakano
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Publication number: 20150010987Abstract: Provided is a method that is for producing Euglena having a high wax ester content, capable of more efficiently producing the Euglena having a high wax ester content by adding a nutrient before anaerobic fermentation to restore the efficiency of wax ester fermentation. The present invention relates to a method for producing Euglena having a high wax ester content. The main configuration of the method is that there is at least a first step of aerobically culturing microalga Euglena under nitrogen starvation conditions and a second step of holding the cells in an anaerobic state; and adding, before the second step, a nutrient source to the culture liquid which has passed through the first step.Type: ApplicationFiled: January 31, 2013Publication date: January 8, 2015Inventors: Ryo Arashida, Yuka Marukawa, Nobuo Aoki, Hitoshi Matsuda, Hiroaki Kato, Akira Yoneda
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Publication number: 20140220638Abstract: Mutant photosynthetic microorganisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutants have a locked in high light-acclimated phenotype, in which many of the photosynthetic parameters characteristic of high light acclimated wild type cells are found in the LIHLA mutants when acclimated to low light, such as reduced chlorophyll, reduced NPQ, higher qP, higher Ek, higher Pmax per unit chlorophyll with little to no reduction in Pmax per cell, and higher rates of electron transport through photosystem II over a wide range of light intensities. Provided herein are constructs for attenuating or disrupting genes are provided for generating mutants having the LIHLA phenotype. Also provided are methods of culturing LIHLA mutants for the production of biomass or other products.Type: ApplicationFiled: December 6, 2013Publication date: August 7, 2014Applicant: Synthetic Genomics, Inc.Inventors: Shaun Bailey, Jay McCarren, Soyan Leung Lieberman, Jonathan E. Meuser, Anna E. Romano, Daniel Yee, Leah Soriaga, Robert C. Brown, Joseph C. Weissman, Roger C. Prince, Robert D. Nielsen, Ariel S. Schwartz
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Patent number: 8735140Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.Type: GrantFiled: January 30, 2012Date of Patent: May 27, 2014Assignee: Kuehnle AgroSystems, Inc.Inventor: Adelheid R. Kuehnle
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Publication number: 20130303752Abstract: Immune function of an animal can be modulated by administration of a composition that includes algae meal or beta glucan. The algae meal can be made by growing Euglena using particular methods and conditions, including certain continuous, semi-continuous, fed-batch, and repeat batch methods in sterile fermenters. Euglena provides a form of beta glucan that is different from other organisms, where the beta glucan is predominantly unbranched beta-1,3-glucan. Use of algae meal and beta glucan produced by the disclosed processes can improve the wellbeing of an animal or human, and may augment or even replace the use of antibiotics in certain circumstances.Type: ApplicationFiled: May 7, 2013Publication date: November 14, 2013Applicant: Algal Scientific CorporationInventor: Algal Scientific Corporation
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Patent number: 8445257Abstract: The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses.Type: GrantFiled: December 28, 2011Date of Patent: May 21, 2013Assignee: Synthetic Genomics, Inc.Inventors: Paul Gordon Roessler, Bo Liu, Jessica Roxane Kristof
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Publication number: 20130115666Abstract: Provided is a method for producing a euglena highly containing a wax ester, comprising aerobically culturing a microalga euglena by photosynthesis using carbon dioxide as a carbon source, then further culturing the microalga euglena under nitrogen-starvation conditions to increase paramylon accumulation per cell, and subsequently putting the microalga euglena under anaerobic conditions, so that a euglena highly containing a wax ester can be produced; and a method for producing a wax ester. The present invention relates to a method for producing a euglena highly containing a wax ester. The method comprises a first step of aerobically culturing a microalga euglena, a second step of further culturing a medium under nitrogen-starvation conditions, and a third step of retaining the cells under anaerobic conditions.Type: ApplicationFiled: July 13, 2011Publication date: May 9, 2013Applicant: EUGLENA CO., LTD.Inventors: Ryo Arashida, Sharbanee Mitra
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Publication number: 20130102055Abstract: A continuous process for efficiently harvesting microalgae from aqueous systems is described herein. The method and apparatus of the present invention allows continuous harvesting of algae from a variety of source waters including saltwater, brackish water, fresh water, and treated wastewater. High concentration factors are achievable and the system produces a deflocculated product that is readily processed for biofuel or pharmaceutical applications. The process of the present invention does not add contaminants that can limit the downstream usage possibilities for the algae concentrate produced. The effluent water from the process is suitable for conventional discharge or recycling to the growth system. The process of the present invention is inexpensive, scalable, and generates useful effluent water and algae concentrate as products.Type: ApplicationFiled: October 20, 2012Publication date: April 25, 2013Applicant: BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEMInventor: BOARD OF REGENTS, THE UNIVERSITY OF TE
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Patent number: 8288150Abstract: A method for breaking the cell walls of microalgae includes cultivating microbes in a cultivating liquid, adding microalgae into the cultivating liquid to mix with the microbes, releasing a hydrolysis ferment from the microbes, hydrolyzing cell walls of the microalgae by the hydrolysis ferment of the microbes to decompose the cell walls of the microalgae into saccharide, and removing the microalgae from the cultivating liquid. Thus, the microbes release the hydrolysis ferment after the microalgae touch the microbes so as to hydrolyze and decompose the cell walls of the microalgae in a moderate manner without breaking the contents of the microalgae so that the contents of the microalgae can be released, absorbed and used completely.Type: GrantFiled: July 9, 2010Date of Patent: October 16, 2012Assignee: Analytica Bioenergy, Inc.Inventors: Jiunn-Jye Chuu, Shun-Lai Li, Hsiao-Hui Hsieh, Chia-Hui Cheng
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Publication number: 20120252105Abstract: Cultivators and methods of cultivating microorganisms are provided. In some examples, a cultivator may include a retaining wall defining a cavity for retaining liquid and may also include a horizontally orientated frame at least partially positioned within the cavity and having media for supporting microorganisms. In other examples, the horizontal frame may be partially submerged in the liquid retained in the cavity and rotatable to selectively submerge and unsubmerge portions of the frame and media.Type: ApplicationFiled: September 22, 2011Publication date: October 4, 2012Applicant: BIOPROCESSH20 LLCInventors: Toby D. Ahrens, Shawn R. Kitchner, John W. Haley, III
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Publication number: 20120225472Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, storage, and production of algae seedstock and uses thereof.Type: ApplicationFiled: January 30, 2012Publication date: September 6, 2012Applicant: KUEHNLE AGROSYSTEMS, INC.Inventor: ADELHEID R. KUEHNLE
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Publication number: 20120220021Abstract: Disclosed herein are transformed non-vascular photosynthetic organisms that are herbicide resistant, nucleotides and vectors useful in conducting such transformations, and transformed strains produced by such transformations.Type: ApplicationFiled: September 15, 2010Publication date: August 30, 2012Applicant: SAPPHIRE ENERGY ,INC.Inventors: Tanya Joy Shin, Yan Poon, Melisa C. Low, Shane Edward Hopkins, Alvin Aram Cho
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Patent number: 8124400Abstract: The present invention provides genes, polypeptides and expression constructs therefor, recombinant photosynthetic microorganisms, and method of use thereof, such as for the production of branched-chain alcohols (including 2-methyl-1-butanol, 3-methyl-1-butanol, and isobutanol) and derivatives thereof for a variety of uses.Type: GrantFiled: December 10, 2009Date of Patent: February 28, 2012Assignee: Synthetic Genomics, Inc.Inventors: Paul Gordon Roessler, Bo Liu, Jessica Roxane Kristof
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Publication number: 20110312063Abstract: The present invention relates to a method for collecting algae from an algae containing aqueous solution. The method comprises first, providing an organic coagulant to said solution and mixing the formed solution. Subsequently, an inert inorganic clay material is provided with mixing to the solution for coagulating said algae. The resulting solution is agitated and the algae is separated and collected from the solution.Type: ApplicationFiled: June 3, 2011Publication date: December 22, 2011Applicant: NESTE OIL OYJInventors: Annika Malm, Reijo Tanner
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Publication number: 20110287544Abstract: Certain embodiments and aspects of the present invention relate to a photobioreactor including photobioreactor units through which a liquid medium stream and a gas stream flow. The photobioreactor units are floated on a body of water such as a pond or a lake. The liquid medium comprises at least one species of phototrophic organism therein. Certain methods of using the photobioreactor system as part of fuel generation system and/or a gas-treatment process or system at least partially remove certain undesirable pollutants from a gas stream. In certain embodiments, the photobioreactor units are formed of flexible, deformable material and are configured to provide a substantially constant thickness of liquid medium. In certain embodiments, a barrier between the photobioreactor unit and the body of water upon which the unit is floated facilitates thermal communication between the liquid medium and the body of water.Type: ApplicationFiled: July 18, 2011Publication date: November 24, 2011Inventors: Isaac Berzin, Javier de Luis, David Fair, Joe Parrish, Keith Richtman, Fan Chen, Benjamin F. Polito, Miles Walker, Ben Fowler, Kevin Lockwood, Miguel Olaizola
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Publication number: 20110020914Abstract: The present invention encompasses a method for enhancing the growth, lipid content and/or protein content of bacteria, microalgae, and fungus grown in an aquatic growth medium.Type: ApplicationFiled: July 16, 2010Publication date: January 27, 2011Applicant: NOVUS INTERNATIONAL INCInventor: Ibrahim Abou-Nemeh
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Publication number: 20100159567Abstract: The present invention relates to compositions and uses of a novel Dunaliella salina HT04 microorganism. In addition, the present invention relates to novel methods for culturing harvesting, preservation, production of algae seedstock and uses thereof.Type: ApplicationFiled: November 9, 2009Publication date: June 24, 2010Inventors: Adelheid R. Kuehnle, Michele Champagne
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Publication number: 20100112649Abstract: Embodiments of the present invention report compositions, methods and uses for growth and/or production of byproducts, products and/or co-products from algal cultures. Certain embodiments of the present invention report using wastewaters to grow microorganisms for producing one or more products, byproducts or co-products from the microorganisms. Other embodiments report compositions and methods for rapid growth of any photosynthetic organism in culture.Type: ApplicationFiled: June 4, 2009Publication date: May 6, 2010Inventors: Bryan Dennis WILLSON, Gail M. BERARDINO-LANG, Amy L. BOCZON, Anna ETTINGER, Nicholas A. RANCIS, Mark Thomas MACHACEK
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Patent number: 7585898Abstract: The present invention relates to a composition for a copious bloom of diatom algae comprising macro and or micro nutrients adsorbed on metalate modified silica sol. The present invention further relates to a process for preparing an aqueous dispersion of a composition comprising of iron adsorbed onto metalate modified silica sol containing 0.01% to 50% of Fe, and a micronutrient adsorbed onto metalate modified silica sol consisting of Mn, Zn, Co, Cu, Mo, B, V, Ni, Se, I, Fl, Cr, Cd and mixtures thereof in the proportion 0.001% to 30% of the weight of silica and macronutrient adsorbed onto metalate modified silica sol consisting of N, P, K, Mg, Ca, S, Cl, Na the elements of each macronutrient varying from 1% to 50% of the silica.Type: GrantFiled: June 7, 2005Date of Patent: September 8, 2009Inventor: Sampath Kumar Thothathri
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Patent number: 7485451Abstract: Storage stable compositions of biological materials, including bioactive biological materials are provided in the form of a water-in-oil emulsion, comprising: (a) cellular material selected from living and/or dormant prokaryotic and/or eukaryotic cells and tissues, the cellular material being compatible with water-in-oil emulsions; (b) one or more oils selected from vegetable oils and fish oils; (c) an oil-soluble nonionic polymeric surfactant having a molecular weight of from about 2500 to about 15000; and (d) water. The compositions may also contain a thickener such as a hydrophobic fumed silica or bentonite. Compositions may be used for various purposes, depending on the contained biological material. Specific examples include compositions containing Fusarium lateritium control of Eutypa lata in plant wounds made by cutting or pruning, and compositions containing Lagenidium giganteum for control of mosquitoes.Type: GrantFiled: November 18, 2004Date of Patent: February 3, 2009Assignee: Regents of the University of CaliforniaInventors: Jean S. VanderGheynst, Herbert B. Scher
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Patent number: 6465240Abstract: A method is provided for treating a waste stream by contacting the waste stream sequentially with a consortium of prokaryotic microorganisms, preferably purple non-sulfur bacteria, followed by a the green algae Chlorella. The consortium of prokaryotic microorganisms assimilate a first portion of the wastes, and the green algae assimilate the remaining portion of the wastes to produce a substantially purified effluent stream. Isolated microorganisms made by the above method are valuable commercial products.Type: GrantFiled: December 11, 1998Date of Patent: October 15, 2002Assignees: Biotechna Environmental International, Ltd.Inventors: Howard M. Wexler, Joseph F. Startari