Abstract: Test strip for the dip and read colorimetric determination of substances dissolved in liquids. Such strips are composed of an inert supporting base covered with a layer of polymer beads in which the reagents are incorporated. The color reaction takes place within the beads themselves which improves sensitivity and reproducibility of the testing.

Abstract: Apparatus for determining a concentration of hydrogen peroxide in an aqueous solution featuring (A) a sampling system, (B) a reaction system, (C) an oxygen concentration determining system, (D) an automatic calibration system and (E) a sequence circuit system. One cycle of determination consists of three operating stages. In the first stage, a zero point adjustment and a span adjustment are established to properly calibrate the sensitivity of an oxygen detector, air saturated water or a fresh sample being used as the standard. In the second stage, a sample is introduced and hydrogen peroxide in the sample is decomposed with a hydrogen peroxide decomposing agent. The concentration of oxygen dissolved in the sample is then determined by means of the oxygen detector. In the third stage, the reaction vessel is washed with fresh water or a fresh sample after the discharging of the used sample. Determinations are repeated at a predetermined time interval under the control of the sequence circuit system.

Abstract: Method, kits and reagents for the simultaneous, kinetic spectrophotometric analysis of blood serum samples for multiple components. Pairs of components which may be simultaneously analyzed are: cholesterol and triglyceride; glucose and urea; uric acid and gamma glutamyl transferase; calcium and magnesium; albumins and total protein.

Abstract: For the determination of glycerol, the latter is incubated with galactose oxidase in an aqueous medium in the presence of oxygen and either the oxygen consumption or the amount of H.sub.2 O.sub.2 or glyceraldehyde that is formed is determined. A reagent suitable for this purpose consists of galactose oxidase and a system for the determination of H.sub.2 O.sub.2 or a system for the determination of glyceraldehyde. It can additionally contain an agent for the saponification of esterified glycerol.

Abstract: For the determination of glycerin by oxidation with oxygen in the presence of glycerinoxidase and measurement of the oxygen consumption or of the H.sub.2 O.sub.2 formation, a glycerinoxidase from Aspergillus spec. DSM 1729 is used. A reagent suitable for this method consists of glycerinoxidase from Aspergillus spec. DSM 1729 and a system for the determination of H.sub.2 O.sub.2 and contains additionally, if desired, an agent for the saponification of esterified glycerin.

Abstract: The present invention provides a process for the determination of .beta.-lactamases by the use of 7-cyanoacetylaminocephalosporanic acid and measurement of the colored material thereby formed, wherein ammonium ions are added to the material to be tested, together with phosphate ions and/or an agent splitting off oxygen.The present invention also provides a reagent for the determination of .beta.-lactamases based upon 7-cyanoacetylaminocephalosporanic acid, which reagent additionally contains ammonium ions, together with phosphate ions and/or an agent splitting off oxygen.

Abstract: This invention relates to a process for determining the uric acid content of biological material by contacting the biological material with a reagent composition comprised of uricase, catalase, aldehyde dehydrogenase, a lower alkanol, and nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotied phosphate, the improvement which comprises determining the uric acid content in the presence of 2-mercaptosuccinic acid.

Abstract: A test tube for the examination of samples, especially of urine samples, in clinical environment for the amount of a given ingredient in the sample, by contacting the sample with a first reagent that reacts with the ingredient in a reaction in which hydrogen peroxide is formed, and with a second reagent which, in cooperation with the so formed hydrogen peroxide, causes an indicator dye to change its color for indicating the amount of the ingredient in the sample, comprises catalase and oxidase contained in at least one layer on at least a part of the inner surface of the test tube, and a solid substance that releases oxygen under the influence of the catalase in the interior of the test tube, the oxidase acting to cause oxidation by the released oxygen of any other ingredient (such as ascorbic acid) of the sample (such as urine sample) which would otherwise react with the formed hydrogen peroxide and thus deleteriously influence the indicator dye color change.

Abstract: There is provided a method for the quantitative analysis of a free fatty acid which comprises (1) the first step of treating a sample containing the free fatty acid with acyl-coenzyme A synthetase in the presence of adenosine triphosphate and coenzyme A to form acyl-coenzyme A, (2) the second step of oxidizing said acyl-coenzyme A, in the presence of oxygen, with acyl-coenzyme A oxidase produced by a microorganism of the genus Candida to form enoyl-coenzyme A and hydrogen peroxide, and (3) the third step of (a) measuring the amount of the formed enoyl-coenzyme A or hydrogen peroxide or (b) measuring the amount of oxygen consumed in said oxidation reaction, to thereby determine the amount of free fatty acid in said sample.

Abstract: A method for the quantitative determination of sugars in a fluid sample. The sugar sample, which may contain free glucose, is passed through a scavenger stage where substantially all of the glucose is removed, so that it does not interfere with the determination. The glucose-free sample is then passed through an immobilized glucose generating stage to produce glucose in molar proportion to the total initial concentration of the sugars present. The generated glucose is passed through an immobilized hydrogen peroxide generating stage to form gluconic acid and hydrogen peroxide. The generated hydrogen peroxide is detected in suitable detection means, typically a polarographic cell. Total free glucose is determined by measuring the total sugars in the sample by bypassing the scavenger stage, determining total sugar, then determining the glucose in the sample without bypassing the scavenger and measuring free glucose as the difference.

Abstract: The invention relates to an improvement in a conventional method for the determination of uric acid involving uricase/catalase/aldehyde dehydrogenase, resulting in the formation of produced NAD(P)H as a measure of the initial uric acid present, which improvement comprises adding at least one compound selected from oxalates, malonic acid mono-lower alkyl esters, trihaloethanols, pyrazole, pyridine, substituted pyrazole and pyridine wherein the substituents are selected from lower alkyl and halogen, pyridine carboxylic acids, pyridine carboxylic acids substituted with a lower radical, pyridine carboxylic acid amides and pyridine carboxylic acid lower alkyl esters, thiourea, isobutyramide and chelate-forming complexing agents, in order to suppress disturbance-causing creep reactions in said method. Reagents containing such additive compounds are also provided.

Abstract: The invention provides a diagnostic agent for the detection of (a) hydroperoxides or of substances which react with the liberation of hydroperoxides or of (b) peroxidase or of peroxidatively-active substances, comprising a stabilized oxidation indicator and, in case (a), peroxidase or a peroxidatively active substance or, in case (b), hydroperoxide or a substance which reacts with the liberation of hydroperoxides; wherein the stabilizer is a 1-arylsemicarbazide of the formulaAr--NH--NH--CO--NH.sub.2in which Ar is aryl or aryl substituted with alkyl, alkoxy or halogen.

Abstract: Glycerol oxidase is an oxidizing enzyme of glycerol characterized by its ability to oxidize glycerol in the presence of oxygen to form hydrogen peroxide and glyceraldehyde. This enzyme is produced by cultivation of a microorganism belonging to the genus Aspergillus or the genus Neurospora in a nutrient medium.

Abstract: A novel procedure for the quantitative determination of hydrogen peroxide concentration in aqueous solutions is described. This is characterized by reacting hydrogen peroxide with an alcohol in the presence of the catalyst catalase and by reacting the resulting aldehyde in the presence of aldehyde dehydrogenase with NAD or NADP followed by determination of NADH or NADPH formed.

Abstract: A method and apparatus is provided for determining the concentration or quantity of enzyme molecules, microorganisms or substrate molecules by monitoring directly the concentration and/or the quantity of vaporous by-product produced from the selective reaction of the molecule catalyzed with a known amount of substrate or of an enzyme or a microorganism. The apparatus comprises a membrane permeable to the vaporous product or substrate, a known amount of microorganism or an enzyme adjacent to the membrane, means for introducing a liquid sample into contact with the microorganism or enzyme within a small volume adjacent the membrane and means for measuring the amount of vaporous by-product passing through the membrane. A mass spectrometer located adjacent the membrane surface is suitable for measuring the amount of vaporous product or substrate passing through the membrane.

Abstract: This invention relates to a method and apparatus for rapid quantitative determination of .alpha.-amylase in aqueous samples such as blood serum, etc. The method comprises a flow-through of the sample through various immobilized reagents contained in sequential stages. A scavenger stage initially removes glucose originally present in the sample and comprises immobilized glucose oxidase and catalase. The glucose-free sample flows through an immobilized starch stage substrate preferably containing a high percentage of amylose to quantitatively react with the .alpha.-amylase in the sample and produce oligosaccharides. The sample with the oligosaccharides flows through a glucose-generating stage containing immobilized glucoamylase which converts the oligosaccharides to glucose. The glucose-containing sample enters a detection stage wherein the glucose is converted to H.sub.2 O.sub.2 by flowing through immobilized glucose oxidase, and the H.sub.2 O.sub.