Involving Oxidoreductase Patents (Class 435/25)
  • Patent number: 10302637
    Abstract: A device for detecting target biomolecules is provided. The device consists of an electrically conductive membrane having a biological recognition component configured to bind a target biomolecule immobilized thereon. The membrane is connected to an electric circuit by means of electrodes. A voltage source applies a voltage to the membrane and a resistance monitoring device monitors the resistance of the membrane as a selected volume of fluid sample suspected of containing the target biomolecule is delivered onto the membrane.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: May 28, 2019
    Assignee: Stellenbosch University
    Inventors: Christiaan Gunter Alwyn Viviers, Willem Jacobus Perold, Leon Milner Theodore Dicks, Giles Hubert Coyle Maybery
  • Patent number: 10294507
    Abstract: Disclosed herein is a device that functions as a glucose sensor. The device has a reference electrode; a counter electrode, a working electrode; an electrically conducting membrane; an enzyme layer; a semi-permeable membrane; a first layer of a first hydrogel in operative communication with the working electrode; the first layer of the first hydrogel being operative to store oxygen; wherein the amount of stored oxygen is proportional to the number of freeze-thaw cycles that the hydrogel is subjected to; and a second layer of the second hydrogel. Disclosed too is a method that comprises using periodically biased amperometry towards interrogation of implantable glucose sensors to improve both sensor's sensitivity and linearity while at the same time enable internal calibration against sensor drifts that originate from changes in either electrode activity or membrane permeability as a result of fouling, calcification and/or fibrosis.
    Type: Grant
    Filed: May 27, 2014
    Date of Patent: May 21, 2019
    Assignee: THE UNIVERSITY OF CONNECTICUT
    Inventors: Fotios Papadimitrakopoulos, Santhisagar Vaddiraju
  • Patent number: 10241121
    Abstract: Antioxidant-containing compositions and methods for confirming antioxidant activity of a composition formulated for topical application to skin. Methods for testing a composition for ability to inhibit both ultraviolet radiation-induced lipid peroxidation on skin and ultraviolet radiation-induced reactive oxygen species formation in the stratum corneum. Compositions and methods for treating and preventing photodamage to skin.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: March 26, 2019
    Assignee: BAYER HEALTHCARE LLC
    Inventors: Thomas A. Meyer, Donathan G. Beasley
  • Patent number: 10221401
    Abstract: Compositions and methods are provided for an O2 tolerant Fe—Fe hydrogenase. The hydrogenases of the invention comprise specific amino acid substitutions relative to the native, or wild-type enzymes.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: March 5, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: James Robert Swartz, Jamin Koo
  • Patent number: 10202455
    Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: February 12, 2019
    Assignee: IMMUNWORK INC.
    Inventors: Tse-Wen Chang, Hsing-Mao Chu
  • Patent number: 10192010
    Abstract: An example method may include identifying (i) a first group of reactions that corresponds to a first set of precursors and a first set of reaction products and (ii) a second group of reactions that corresponds to a second set of precursors and a second set of reaction products. No precursor in the first set of precursors is also in the second set of precursors, no reaction product in the first set of reaction products is also a precursor in the second set of precursors, and no reaction product in the second set of reaction products is also a precursor in the first set of precursors. The method may also include executing a first processing thread to iteratively calculate respective quantities of the precursors in the first set of precursors and executing a second processing thread to iteratively calculate respective quantities of the precursors in the second set of precursors.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: January 29, 2019
    Assignee: X Development LLC
    Inventors: Jonathan Ross, Jason Thompson
  • Patent number: 10050214
    Abstract: The present invention relates to a near-ultraviolet stimulated light-emitting compound, and more specifically relates to a fluorescent quinazolinone compound which is stimulated in the near ultraviolet region. The fluorescent quinazolinone compound according to the present invention is colorless in daylight but exhibits a high fluorescent intensity when irradiated with light in the near ultraviolet region. Also, the fluorescent quinazolinone compound can be easily used with printed and plastic molded products since the compound not only has a high degree of light fastness and thermal stability but also high dispersibility.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: August 14, 2018
    Assignees: NANO CMS CO., LTD., KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY
    Inventors: Je Young Park, In Ja Lee, Shi Surk Kim, Kun Jun, Seung Rim Shin, Kyoung Lyong An
  • Patent number: 10031119
    Abstract: Colorimetric explosive detection kits comprising compact and flexible packaging with an explosive collection assembly and detection reagents disposed thereon in a single-easy-to use system.
    Type: Grant
    Filed: November 5, 2015
    Date of Patent: July 24, 2018
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Christopher D. Applegate, Joseph M. Laquidara
  • Patent number: 10023900
    Abstract: The invention relates to method for quantification of the absolute amount of allergen in an allergen sample comprising: a) providing a known amount of one or more allergen calibration standard peptide(s) having a sequence of amino acids which is identical with, and optionally unique for, a sequence to be found in the allergen to be quantified and optionally labelling said allergen calibration standard peptide(s), b) degrading the allergen sample to obtain a mixture of peptides, and optionally labelling said peptides with one or more labelling agent(s), wherein at least the peptides in the degraded allergen sample or the calibration standard peptides are labelled, and if both the peptides in the degraded allergen sample and the allergen calibration standard peptide(s) are labelled, the labelling agent(s) used for labelling the allergen calibration standard peptide(s) are different from the labelling agent(s) used for labelling the peptides of the degraded allergen sample, c) quantifying the absolute amount
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: July 17, 2018
    Assignee: ALK-ABELLO A/S
    Inventor: Ulla Seppälä
  • Patent number: 10011842
    Abstract: In this invention, cell lines are created for enzyme inhibitory testing of inhibitors against Plasmodium falciparum DHFR-TS and HPPK-DHPS. Provided the complementing DHFR-TS and HPPK-DHPS have sufficient activities to support growth of the surrogates in un-supplemented medium, the same surrogates could be used for screening inhibitors of targets against other parasite and pathogen species e.g. Plasmodium vivax, Trypanosoma brucei, Trypanosoma cruzi, Toxoplasma gondii or Mycobacterium tuberculosis. The cell lines in this invention are Escherichia coli strain whose thyA, folA, folK, and folP genes were disrupted using genetic knockout coupled with elimination of antibiotic resistance markers. The thyA KO, folP KO, folK KO, thyAfolA KO, folKfolP KO, thyAfolAfolP KO, thyAfolAfolK KO and thyAfolAfolKfolP KO E.
    Type: Grant
    Filed: September 18, 2014
    Date of Patent: July 3, 2018
    Assignee: National Science and Technology Development Agency
    Inventors: Nattida Suwanakitti, Yuwadee Talawanich, Yongyuth Yuthavong, Sumalee Kamchonwongpaisan
  • Patent number: 9984278
    Abstract: The present invention generally relates to non-linear imaging systems and methods for use in various assisted reproductive technologies and other applications. In one aspect, the present invention is generally directed to two-photon and other non-linear microscope techniques that can be used to determine the status or maturity state of eggs or egg follicles, for example by determining the presence of polar bodies within the egg follicles. In some embodiments, endogenous metabolites, such as NADH or FAD, can be determined, and in some cases without externally altering those metabolite concentrations or without adding other compounds. Other embodiments of the invention are also directed to computer programs or systems that can be used to determine the status or maturity state of eggs or egg follicles, kits in such systems, or the like.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: May 29, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Daniel Needleman, Manqi Deng, Timothy H. Sanchez
  • Patent number: 9975951
    Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: May 22, 2018
    Inventors: Tse-Wen Chang, Hsing-Mao Chu
  • Patent number: 9834578
    Abstract: The present disclosure provides methods and composition involving increased sensitivity of compounds using mass spectrometry. In one embodiment, a method of increasing the sensitivity for detection of a carbonyl group-containing compound by mass spectrometry can comprise derivatizing the carbonyl group-containing compound with an O-substituted hydroxylamine thereby producing an oxime, resulting in enhanced sensitivity of detection by mass spectrometry, as compared to the underivatized carbonyl group-containing compound. Additionally, a method for assaying a carbonyl group-containing compound can comprise reacting the carbonyl group-containing compound in a sample with an O-substituted hydroxylamine to produce an oxime and performing analysis with mass spectrometric detection of the oxime by a mass spectrometry instrument.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: December 5, 2017
    Assignee: University of Utah Research Foundation
    Inventors: Bingfang Yue, Alan L. Rockwood, Mark M. Kushnir
  • Patent number: 9816127
    Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: November 14, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Fen Huang, Dieter Klaubert, John Shultz, Wenhui Zhou
  • Patent number: 9795962
    Abstract: The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: October 24, 2017
    Assignee: Abbott Point of Care Inc.
    Inventor: Cary James Miller
  • Patent number: 9730935
    Abstract: Methods and compositions are provided for treating an individual having cancer. Aspects of the methods include administering to the individual an inhibitor of a non-canonical Notch signaling pathway gene in an amount effective to treat the cancer. Also provided are reagents, devices and kits thereof that find use in practicing the subject methods.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: August 15, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Bingwei Lu, Kyu-Sun Lee
  • Patent number: 9671348
    Abstract: The present invention provides a method for measuring a component to be measured in a sample, comprising converting the component to be measured in the sample into hydrogen peroxide and measuring the formed hydrogen peroxide in the presence of an ?-keto acid using an oxidative-coloring chromogen. The present invention also provides a method for suppressing the influence of a peroxide on a method of converting a component to be measured in a sample into hydrogen peroxide and measuring the formed hydrogen peroxide using an oxidative-coloring chromogen, the suppression method comprising using an ?-keto acid. The measuring method and the suppression method of the present invention using an ?-keto acid suppress the influence of a peroxide to give an accurate measurement of the component to be measured in the sample.
    Type: Grant
    Filed: December 12, 2011
    Date of Patent: June 6, 2017
    Assignee: KYOWA MEDEX CO., LTD.
    Inventor: Tomomi Murakami
  • Patent number: 9642922
    Abstract: Provided is an activatable probe that undergoes intramolecular cyclization and subsequent aggregation in apoptotic tumor cells upon peptidase-initiated, and most advantageously caspase-3, activation. These caspase-sensitive nano-aggregation probes (C-SNAFs) are generally biocompatible, possess NIR spectral properties or may serve as PET or MRI imaging agents, and have a mechanism of target-mediated nanostructure self-assembly amenable to in vivo use. The probes encompass biocompatible condensation chemistry products that comprise D-cysteine and 2-cyano-6-hydroxyquinoline (CHQ) moieties linked to an amino-luciferin scaffold, and which can be activated by a two-step reaction requiring caspase-3/7-mediated cleavage of an aspartate-glutamate-valine-aspartate (L-DEVD) capping peptide and the free intracellular thiol-mediated reduction of the disulfide bond.
    Type: Grant
    Filed: August 20, 2014
    Date of Patent: May 9, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jianghong Rao, Deju Ye, Adam Shuhendler, Frederick Te-Ning Chin, Jongho Jeon, Bin Shen
  • Patent number: 9550979
    Abstract: The present invention relates to mutant alcohol dehydrogenase enzymes, microorganisms comprising the mutant alcohol dehydrogenase enzymes, and methods for the production of products by microbial fermentation using the microorganisms.
    Type: Grant
    Filed: April 5, 2013
    Date of Patent: January 24, 2017
    Assignee: Lanza Tech New Zealand Limited
    Inventors: Michael Koepke, Wayne Michael Patrick, Danielle Joan Maddock, Monica Gerth
  • Patent number: 9528990
    Abstract: The invention relates to fluorescent/fluorogenic probes of formula (I?) or (II?): wherein Z is chosen between: —NH2 and —OH, or in the fluorescence quencher group consisting of: —NO2; —N?N—R1; R1 being any organic group that does not obscure the corresponding azo bond; —NHCO-Pept.; Pept. being a peptide residue or any organic group that does not obscure the corresponding amide bond; —O-Glyc.; Glyc. being a oligoglycoside residue that does not obscure the corresponding glycosidic bond; —O—C(O)—R2; —O—P(O)(OR2)(OR2?) and —O—S(O)2—R2; R2 and R2? being independently a hydrogen atom or an organic group that does not obscure the corresponding ester bond; and ?Ra, Rb, Rc, Rd and Re being independently a hydrogen atom or any organic group that does not obscure the corresponding arylether bond so as not to prevent its possible cleavage by a myeloperoxidase activity.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: December 27, 2016
    Assignees: BIOMERIEUX S.A., CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE ROUEN
    Inventors: Sylvain Orenga, Valérie Chalansonnet, Arnaud Chevalier, Pierre-Yves Renard, Anthony Romieu, Benoit Roubinet
  • Patent number: 9506939
    Abstract: Provided herein are assay control materials comprising stable analytes and lyophilized unstable analytes, and methods of making and using the same.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: November 29, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Alireza Ebrahim, Karl De Vore, Christopher Spates
  • Patent number: 9458491
    Abstract: The present invention provides a method for measuring the concentration of an analyte in a test solution wherein the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1 and an enzyme that catalyzes a reaction represented by Reaction Formula 2 to act on a test solution containing mevalonic acid and/or 3 -hydroxymethylglutaryl coenzyme A in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same.
    Type: Grant
    Filed: January 19, 2010
    Date of Patent: October 4, 2016
    Assignee: ASAHI KASEI PHARMA CORPORATION
    Inventor: Takeshi Matsuoka
  • Patent number: 9394562
    Abstract: The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.
    Type: Grant
    Filed: January 2, 2015
    Date of Patent: July 19, 2016
    Assignees: AMOREPACIFIC CORPORATION, SNU R&DB FOUNDATION
    Inventors: Jun Seong Park, Duck Hee Kim, Han Kon Kim, Eun Mi Kim, Byung Gee Kim
  • Patent number: 9366645
    Abstract: Embodiments of the present invention provide binding molecule-functionalized high electron mobility transistors (HEMTs) that can be used to detect toxins, pathogens and other biological materials. In a specific embodiment, an antibody-functionalized HEMT can be used to detect botulinum toxin. The antibody can be anchored to a gold-layered gate area of the HEMT through immobilized thioglycolic acid. Embodiments of the subject detectors can be used in field-deployable electronic biological applications based on AlGaN/GaN HEMTs.
    Type: Grant
    Filed: January 7, 2014
    Date of Patent: June 14, 2016
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Fan Ren, Stephen John Pearton, Tanmay P. Lele
  • Patent number: 9327230
    Abstract: A gas cleaning system including a wet scrubber (14) for removal of sulphur dioxide from a gas and an oxidation basin system (42) for receiving effluent generated in the removal of sulphur dioxide from the process gas. The oxidation basin system (42) comprises an oxidation basin (43) for containing the effluent during treatment thereof, an oxygen supply system (47) for supplying oxygen to the effluent in the oxidation basin (43), and a contacting system for bringing the effluent into contact with an enzyme which catalyzes the oxidation of bisulphite and/or sulphite ions.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: May 3, 2016
    Assignee: ALSTOM Technology Ltd
    Inventors: Fredrik Jens Brogaard, Mikael Larsson
  • Patent number: 9302227
    Abstract: A membrane separation assembly that includes an integrated filter element and at least one membrane module housed within a first vessel and a second vessel containing at least one membrane module, which is stacked or aligned adjacent to the first vessel. The first vessel is configured to allow liquids to be trapped and removed from the assembly, and gases to flow to and through the membrane modules of the first vessel and the membrane modules of the second vessel, which are ultimately withdrawn from the assembly. The assembly is useful in the conditioning of fuel gas to separate methane from C2+ hydrocarbons.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: April 5, 2016
    Assignee: Membrane Technology and Research, Inc.
    Inventor: Kaaeid A. Lokhandwala
  • Patent number: 9284599
    Abstract: A method of testing for the presence of a preselected target nucleic acid, protein or antigen in a biological sample by exposing nucleic acids, proteins or antigens to a probe having a catalytic element and binding element. The catalytic element catalyses at least one reaction that results in a physical change such that identifiable elements provide an indication of the presence of the target.
    Type: Grant
    Filed: March 4, 2011
    Date of Patent: March 15, 2016
    Assignee: Eluceda Limited
    Inventors: Ian Eastwood, Andrew Reid, Mark Saw
  • Patent number: 9284596
    Abstract: Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: March 15, 2016
    Assignee: Battelle Energy Alliance, LLC
    Inventors: David Neil Thompson, Emily DeCrescenzo Henriksen, David William Reed, Jill Renee Jensen
  • Patent number: 9279127
    Abstract: The present invention is directed to constructs, compositions and methods for modulating platelet-type 12 lipoxygenase (12-LO) in adipose tissue in vivo. Specifically, the invention provides constructs encoding expression-inhibiting oligonucleic acids, e.g., antisense and RNA interfering (RNAi) molecules, targeted to a platelet-type 12-LO gene or a transcript thereof, which are capable of reducing or silencing platelet-type 12-LO expression specifically in adipocytes and pre-adipocytes. Vectors of these constructs (including, but not limited to, viral vectors), compositions of them and methods of using same for the treatment and amelioration of conditions associated with excess fat cell mass and obesity are also provided.
    Type: Grant
    Filed: November 1, 2007
    Date of Patent: March 8, 2016
    Assignee: The Medical Research Fund at the Tel-Aviv Sourasky Medical Center
    Inventors: Gary Weisinger, Rona Limor, Naftali Stern
  • Patent number: 9179869
    Abstract: The invention provides an implantable membrane for regulating the transport of analytes therethrough that includes a matrix including a first polymer; and a second polymer dispersed throughout the matrix, wherein the second polymer forms a network of microdomains which when hydrated are not observable using photomicroscopy at 400× magnification or less. In one aspect, the homogeneous membrane of the present invention has hydrophilic domains dispersed substantially throughout a hydrophobic matrix to provide an optimum balance between oxygen and glucose transport to an electrochemical glucose sensor.
    Type: Grant
    Filed: September 10, 2014
    Date of Patent: November 10, 2015
    Assignee: DexCom, Inc.
    Inventors: Mark A. Tapsak, Rathbun K. Rhodes, Mark C. Shults, Jason D. McClure
  • Patent number: 9063119
    Abstract: Provided are methods for determining the amount of vitamin C in a sample using mass spectrometry. The methods generally involve ionizing vitamin C in a sample and detecting and quantifying the amount of the ion to determine the amount of vitamin C in the sample.
    Type: Grant
    Filed: June 16, 2014
    Date of Patent: June 23, 2015
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Qibo Jiang, Richard E Reitz, Sum Chan
  • Publication number: 20150140124
    Abstract: The present invention relates to the BAD pathway's influence on development, progression, chemo-sensitivity, and overall survival for multiple human cancers and its potential as a therapeutic target to increase chemo-sensitivity. BAD pathway expression was associated with the development and/or progression of breast, colon, and endometrial cancers, relapse-free survival from breast cancer, and overall survival from ovarian, colon, and brain cancers. Expression was also associated with in vitro sensitivity to a range of cytotoxic agents. pBAD levels were higher in cancer versus immortalized normal cells and chemo-resistant versus—sensitive cancer cells and associated with increased cell proliferation.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 21, 2015
    Inventors: Johnathan M. Lancaster, Douglas C. Marchion, Yin Xiong
  • Publication number: 20150140560
    Abstract: The disclosure provides a label-free viscosity-based analyte detection system using paramagnetic beads as an asynchronous magnetic bead rotation (AMBR) microviscometer. It is disclosed herein that the bead rotation period is linearly proportional to the viscosity of a solution comprising analytes surrounding the paramagnetic bead. Optical measurement of asynchronous microbead motion determines solution viscosity precisely in microscale volumes, thus allowing an estimate of analyte concentration. The results demonstrate the feasibility of viscosity-based analyte detection using AMBR in microscale aqueous volumes.
    Type: Application
    Filed: November 4, 2014
    Publication date: May 21, 2015
    Inventors: Raoul Kopelman, Mark A. Burns, Yunzi Li
  • Publication number: 20150141385
    Abstract: Methods for diagnosing, treating, and preventing catabolism-related vitamin D deficiency and related disorders, related compositions, apparatus and kits, are disclosed. A method involves measuring CYP24 expression and/or activity, or a proxy thereof such as FGF23 level, in a patient and correlating abnormally elevated CYP24 expression and/or activity with catabolism-related vitamin D deficiency or with susceptibility for catabolism-related vitamin D deficiency. In response to abnormally elevated CYP24 expression and/or activity, the method further includes administering a CYP24 inhibitor to the vitamin D deficient or at-risk patient, and preferably avoiding activation of the vitamin D binding receptor, such as by avoiding administration of active vitamin D compounds to such patients. Optionally, a vitamin D prohormone or prohormone can be administered.
    Type: Application
    Filed: January 28, 2015
    Publication date: May 21, 2015
    Inventors: P. Martin Petkovich, Christian F. Helvig, Joel Z. Melnick
  • Publication number: 20150140591
    Abstract: The invention concerns the field of cell culture technology. It concerns RNA having a specific sequence, expression vectors encoding said RNA, production host cell lines comprising said RNA, and methods of producing recombinant biopharmaceutical products using engineered host cell with altered levels of said RNAs, such as small non-coding RNAs, preferably microRNAs (miRNAs). The invention also relates to engineered host cells with altered levels in one or more of said RNAs. Those cell lines have improved secretion and/or growth characteristics in comparison to control cell lines.
    Type: Application
    Filed: June 4, 2013
    Publication date: May 21, 2015
    Inventors: Lore Florin, Hitto Kaufman, Angelika Hausser, Monilola Olayioye, Michaela Strotbek
  • Publication number: 20150140592
    Abstract: Methods and compositions are provided for engineering microorganisms, which permit enhanced H2 production. The methods and compositions provided include novel chimeric gene constructs encoding H2-forming H2ase and maturation proteins, allowing for generation of H2 continuously in large quantities. In one illustrated embodiment, novel engineered algae are provided with increased levels of H2 production.
    Type: Application
    Filed: November 14, 2014
    Publication date: May 21, 2015
    Inventor: Svetlana Oard
  • Patent number: 9034593
    Abstract: A diagnostic kit for biomarkers of beneficial vaginal health is described. The kit includes a first detection zone within which a D-lactic acid detection signal is capable of being generated, wherein the presence or quantity of D-lactic acid, is determinable from the D-lactic acid detection signal. The kit further includes a second detection zone within which a hydrogen peroxide detection signal is capable of being generated, wherein the presence or quantity of hydrogen peroxide, is determinable from the hydrogen peroxide detection signal.
    Type: Grant
    Filed: November 22, 2010
    Date of Patent: May 19, 2015
    Assignee: Kimberly-Clark Worldwide, Inc.
    Inventors: Stephanie M. Martin, Ronnie L. Phillips, SangWook Lee, JunMo Gil
  • Publication number: 20150129425
    Abstract: An enzyme electrode includes an electrode, and a detection layer that is in contact with the electrode and includes an oxidoreductase, a water-soluble conductive polymer, and conductive particles, electrons being transferred between the enzyme and the electrode by direct electron transfer in the detection layer.
    Type: Application
    Filed: June 25, 2013
    Publication date: May 14, 2015
    Applicant: BIOENGINEERING LABORATORIES, LLC
    Inventors: Masashi Tsukada, Mayumi Tajima
  • Patent number: 9029098
    Abstract: A date-rape drug detector includes an elongated shaft having an upper end and a lower end. The upper end of the shaft is bendable and includes a plurality of notches that grip the upper rim of a beverage container. Proximal the lower end is a chamber having a testing strip therein that is deployable to a perpendicular position relative to the shaft. Along the length of the strip are a plurality of reactive spots that change color in the presence of a date-rape drug. Each successive spot is covered with a progressively thicker layer of water-soluble material than a preceding spot so that each spot requires a different submersion time in order to be exposed. Therefore, a user can test a beverage for the presence of a date-rape drug at progressive intervals.
    Type: Grant
    Filed: January 15, 2014
    Date of Patent: May 12, 2015
    Inventor: Kathy Barbosa Holcombe
  • Publication number: 20150125889
    Abstract: The present invention relates to the use of at least one azo compound for detecting at least one microorganism in a sample. More precisely, the present invention relates to a process for detecting, in a biological sample, at least one microorganism with azoreductase activity, including the steps: placing the sample in contact with a reaction medium including at least one azo compound, incubating the reaction medium, and detecting the reduction of the azo compound by the microorganism, indicating the presence of the at least one microorganism.
    Type: Application
    Filed: January 18, 2013
    Publication date: May 7, 2015
    Inventors: Arthur James, Céline Roger-Dalbert, Claire Mercier, Sylvain Orenga
  • Publication number: 20150118702
    Abstract: The present invention discloses functional nitrite reductase as a potential drug target for anti-tubercular drug development. The present invention also relates to the development of an easy method for identification of nitrite in clinical samples as well as its correlation with the severity of the disease. Presence of active as well as dormant/latent stages of Mycobacterium tuberculosis (MTB) could be identified from nitrite in clinical samples like sputum of potential TB patients.
    Type: Application
    Filed: November 3, 2014
    Publication date: April 30, 2015
    Inventor: Dhiman Sarkar
  • Publication number: 20150111228
    Abstract: The present disclosure provides an optical biosensor including a complex including a porous support and an enzyme supported inside the pores of the porous support and a method for preparing the same. Since a dye does not flow but is concentrated in one place, the optical biosensor of the present disclosure has remarkably improved sensitivity. In addition, it is possible to carry out quantitative determination and qualitative analysis since color intensity increases with time.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 23, 2015
    Applicants: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Jungbae Kim, Min-Gon Kim, Hyou-Arm Joung, Yongho Wee
  • Patent number: 9012169
    Abstract: The invention is concerned with the identification of compounds as flavors or fragrances, their precursors, or as modulators of the perception of fragrances or flavors. The method comprises a reaction of a compound with a metabolic enzyme expressed in the nose, mouth or respiratory tract, followed by a method of identification of the compound or its metabolites as a fragrance or flavor, their precursor, or a modulator of their perception or of the perception of their respective leads.
    Type: Grant
    Filed: July 15, 2005
    Date of Patent: April 21, 2015
    Assignee: Givaudan S.A.
    Inventor: Boris Schilling
  • Publication number: 20150101929
    Abstract: The present invention relates to a stabilizing composition useful for improving the stability of reagent for redox reaction, and a reagent composition for redox reaction having an improved stability. The reagent composition for redox reaction can be applied for a reagent for electrochemical biosensor.
    Type: Application
    Filed: January 25, 2013
    Publication date: April 16, 2015
    Inventors: Sung-Kwon Jung, Moon Hwan Kim, Eun Hye Im, Myeongho Lee, Ung Ki Lee, Geun Sig Cha, Hakhyun Nam
  • Patent number: 9005914
    Abstract: A device and method are disclosed for use in the conduct of a non-invasive analysis of a bodily fluid to determine the presence and level of a certain constituent carried by the bodily fluid, which analysis utilizes an indicator formulation that changes color in response to exposure to the constituent to provide a visible indication of the presence and level of the constituent carried by the bodily fluid. A stabilizing formulation is carried in a vessel for mixing with a sample of the bodily fluid to be analyzed. The stabilizing formulation includes a first component for promoting formation of a film, a second component for adjusting the pH of the sample and inhibiting microbial digestion of the certain constituent carried by the bodily fluid, and a third component for reducing interference of ascorbic acid present in the bodily fluid. Where the certain constituent is glucose, the stabilizing formulation includes a microbial digestion inhibitor.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: April 14, 2015
    Assignee: Pop Test LLC
    Inventors: Randice Lisa Altschul, Myron Rapkin, Rebecca O'Brien
  • Publication number: 20150093354
    Abstract: In various embodiments, the present disclosure provides a method and enzyme for forming various compounds, such as monoterpenes and monoterpenoid compounds. In a specific example, the present disclosure provides a method for producing one or more of (?)-ipsdienol, (?)-ipsenol, ipsenone, and ipsdienone. The present disclosure also provides methods of using compounds formed from the disclosed method and enzyme.
    Type: Application
    Filed: December 8, 2014
    Publication date: April 2, 2015
    Applicant: Board of Regents of the Nevada System of Higher Education, on Behalf of the University of Nevada
    Inventors: Claus Tittiger, Rubi Figueroa-Teran, Gary J. Blomquist
  • Patent number: 8993255
    Abstract: The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from Phaeosphaeria nodorum, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.
    Type: Grant
    Filed: October 6, 2009
    Date of Patent: March 31, 2015
    Assignees: Toyo Boseki Kabushiki Kaisha, Gekkeikan Sake Co., Ltd.
    Inventors: Rie Hirao, Masao Kitabayashi, Yoshiaki Nishiya, Hiroki Ishida, Yoji Hata
  • Patent number: 8993313
    Abstract: Provided are an analytical instrument and an analytical method that allow for the direct analysis of a target substance in an undiluted specimen by a transmission photometry in a tightly closed cell container space. An analytical instrument is an analytical instrument for analyzing a target substance contained in a specimen flown in the tightly closed cell container by utilizing an oxidative color-developing agent and an oxidative enzyme reaction, in which an upper substrate and a lower substrate are arranged facing each other and at least a part of the upper substrate and/or at least a part of the lower substrate are/is made of a material transmitting light used for the analysis and having oxygen transmission properties.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: March 31, 2015
    Assignee: ARKRAY, Inc.
    Inventor: Toshihiro Imai
  • Publication number: 20150087551
    Abstract: Apparatus, systems, and methods for detecting, screening and sampling of cells are disclosed.
    Type: Application
    Filed: July 22, 2014
    Publication date: March 26, 2015
    Inventor: Rolf U. Halden
  • Publication number: 20150079619
    Abstract: An analysis method determines a functional parameter of an organ by measuring 13CO2 content in exhaled air of an individual to which a substrate has been administered. The reaction of the substrate in the body of the individual enriches the exhaled air with 13CO2. The maximum reaction rate of the substrate in the body is determined via a change of the measured 13CO2 content in the exhaled air using zero-order enzyme kinetics. An aqueous methacetin solution having a pH greater than 7.0 is used in the analysis method. A face mask separates the exhaled air from inhaled air. The face mask is configured such that inhaled and exhaled air flows essentially completely through the face mask. Exhalation and inhalation valves in the face mask allow a flow of inhaled and exhaled air through the face mask. A diagnostic method is used to determine the functional parameters of the organ.
    Type: Application
    Filed: November 25, 2014
    Publication date: March 19, 2015
    Inventors: Martin STOCKMANN, Björn RIECKE