Involving Oxidoreductase Patents (Class 435/25)
  • Patent number: 11513114
    Abstract: A method of choosing which undesired cell to destroy in a multi-cell fluorescent event includes detecting fluorescence of cells, converting photons detected in the fluorescence into an analog voltage output signal, and identifying at least two discernable peaks associated with the cells. By looking solely at properties measured within the multi-cell fluorescent event, a decision of which cell to target for elimination can be made. Using this method with large population sizes can result in an effective sex skewed product. The sex skewed product can, for example, be formed from bull semen which is then later used to inseminate cows which results in an increased likelihood of giving birth to female cattle.
    Type: Grant
    Filed: May 29, 2020
    Date of Patent: November 29, 2022
    Assignee: ABS Global, Inc.
    Inventors: Frederick Savage, Matthew Ebersole, Daniel McAda, David Appleyard, Zheng Xia
  • Patent number: 11499143
    Abstract: Provided are an amadoriase that is less likely to be influenced by oxygen concentration and a method and a reagent kit for measurement of HbA1c using such amadoriase. Provided are an amadoriase that is obtained via substitution of one or more amino acid residues at a position or positions corresponding to the position(s) selected from the group consisting of positions 280, 267, 269, 54, and 241 of the amadoriase derived from the genus Coniochaeta, a method for measurement of HbA1c, a reagent kit for measurement, and a sensor using such amadoriase. The modified amadoriase according to the invention has a lowered oxidase activity and an enhanced dehydrogenase activity, and this enables the use of an electron mediator, and this reduces the influence of oxygen concentration. Thus, HbA1c can be measured with high sensitivity.
    Type: Grant
    Filed: October 23, 2015
    Date of Patent: November 15, 2022
    Assignee: Kikkoman Corporation
    Inventors: Yosuke Masakari, Airi Komatsuzaki, Atsushi Ichiyanagi
  • Patent number: 11435341
    Abstract: The present invention provides rapid and simple methods of testing on whole blood samples for alerting cancer recurrence and assessing cancer progression relying on determination of the functionality of leucocytes (predominately neutrophils) to exhibit challenge-induced superoxide anion production with quantification by chemiluminescent measurement.
    Type: Grant
    Filed: October 2, 2017
    Date of Patent: September 6, 2022
    Assignee: SEROXO LIMITED
    Inventors: David Sarphie, Rubina Mian
  • Patent number: 11384381
    Abstract: Provided is a compound which accelerates the enzymatic reaction catalyzed by an oxidase. The present invention provides an oxidase reaction accelerating agent comprising a compound represented by formula (I) and a method using the same.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: July 12, 2022
    Assignee: Kikkoman Corporation
    Inventors: Atsushi Ichiyanagi, Kazuhiko Shimoji
  • Patent number: 11372007
    Abstract: Provided is a method of detecting a biological material, by which quantitative measurement can be performed easily. The method of detecting a biological material in a sample includes: mixing, with the sample, a fusion protein (C) in which a protein (A) capable of binding the biological material and a chemiluminescent protein (B) are fused together and a substrate for the chemiluminescent protein (B); and observing a luminescent signal from the sample, wherein the protein (A) and the protein (B) are linked in such a manner that resonance energy transfer can occur, the protein (A) is either a protein (A1) that can emit fluorescence in a state where the biological material is bound thereto or a protein (A2) capable of binding an autofluorescent molecule as the biological material, and the protein (B) can excite fluorescence or autofluorescence of the protein (A) with its luminescence energy.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: June 28, 2022
    Assignee: Osaka University
    Inventors: Takeharu Nagai, Yoshiyuki Arai
  • Patent number: 11344223
    Abstract: The present invention relates to a method and device for measuring acetone concentrations in breath. Measuring acetone concentrations is based on changes the spectral properties of the fluorophore caused by the chemical interaction of a specific fluorophore with the exhaled air containing acetone which.
    Type: Grant
    Filed: September 16, 2020
    Date of Patent: May 31, 2022
    Inventors: Slava Lebov, Sergey Babichenko, Alexander Dudelzak, Aleksei Kuznetsov, Innokenti Sobolev, Leino Vint
  • Patent number: 11220704
    Abstract: Provided is a method of avoiding the influence of a coexisting substance in the measurement of HbA1c % for a whole blood sample by an enzymatic method. Specifically, provided is a method of measuring a ratio of a hemoglobin A1c concentration to a hemoglobin concentration in a sample by an enzymatic method, the method including: a first step of optically measuring the hemoglobin concentration; and a second step of optically measuring the hemoglobin A1c concentration, wherein, when HbA1c % is calculated by dividing the hemoglobin A1c concentration measured in the second step by the hemoglobin concentration measured in the first step, the hemoglobin concentration serving as a denominator, which is measured at a first wavelength, is corrected using a result measured at a second wavelength.
    Type: Grant
    Filed: August 10, 2017
    Date of Patent: January 11, 2022
    Assignee: SEKISUI MEDICAL CO., LTD.
    Inventors: Satoshi Machida, Tomohisa Nishio, Kazuo Nakanishi
  • Patent number: 11198852
    Abstract: The present invention provides a composition that enables measurement of glycated hemoglobin in the presence of a stronger surfactant than conventional surfactants. To this end, the present invention provides an amadoriase in which one or more amino acids have been substituted at positions corresponding to positions selected from the group consisting of positions 80, 71, 175, 172, 279, 12, 9, 77, 30, 28, 13, 3, 4, 286, 204, 338, 44, 340, and 194 of the amadoriase derived from the genus Coniochaeta having the amino acid sequence as shown in SEQ ID NO: 1 as well as a composition for measurement of glycated hemoglobin comprising an amadoriase that retains activity in the presence of an anionic surfactant. The present invention can provide an enzyme and a composition for measurement of glycated hemoglobin that sufficiently remain stable even when exposed to anionic surfactants.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: December 14, 2021
    Assignee: Kikkoman Corporation
    Inventors: Yosuke Masakari, Airi Komatsuzaki
  • Patent number: 10969358
    Abstract: A method of calibrating a device for measuring the concentration of creatinine in a sample including one or more enzyme modulators, the device comprising an enzyme layer, the method comprising: determining sensitivities of the device for each of one or more calibration solutions; determining a degree of modulation for the sample to be measured, determining a degree of modulation for each calibration solution; wherein said determining of each of the degrees of modulation comprises estimating the concentration of an enzyme modulator in the enzyme layer of the device; and calculating the sensitivity of the device for the sample, wherein the said calculating comprises adjusting the sensitivity of the device for each calibration solution by a factor comprising the determined degrees of modulation of the sample and the calibration solution.
    Type: Grant
    Filed: June 20, 2016
    Date of Patent: April 6, 2021
    Assignee: RADIOMETER MEDICAL APS
    Inventors: Thomas Steen Hansen, Thomas Pedersen Nygaard
  • Patent number: 10962556
    Abstract: A method of measuring and calculating (preferably by a computer with output to a user) tGSH (total glutathione very particularly defined) with sample preparation and assay methods that have been confirmed to provide accurate and reliable tGSH and related diagnostic assays in blood or tissue from a patient.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: March 30, 2021
    Assignee: Definitek, Inc.
    Inventors: Howard M. Kingston, Mehmet Pamukcu
  • Patent number: 10913937
    Abstract: A composition comprised of a perodixase, a catalase and a peroxide that, in combination, is suitable for degradation of pollen wall sporopollenin, and coatings comprising sporopollenin in a matrix that can be selectively degraded through application of a peroxidase, a catalase and a peroxide to the sporopollenin matrix.
    Type: Grant
    Filed: April 17, 2018
    Date of Patent: February 9, 2021
    Assignee: Lafayette College
    Inventor: Anna F. Edlund
  • Patent number: 10907191
    Abstract: The present invention relates to a method for measuring activity of HAO which comprises bringing HAO into contact with hydroxylamine in the presence of a tetrazolium salt. Further, the present invention also provides a method for culturing microorganisms which comprises culturing microorganisms in a storage container of a sample containing microorganisms installed separately in a liquid medium storage container, wherein the storage container of a sample containing microorganisms has pores.
    Type: Grant
    Filed: January 18, 2017
    Date of Patent: February 2, 2021
    Assignee: NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
    Inventors: Toshimasa Yamazaki, Yuki Nishigaya
  • Patent number: 10871484
    Abstract: Polyaromatic hydrocarbons (PAH) from industrial waste and oil spills are a recurrent threat to food safety due to their stability and toxicity. Existing methods to establish the concentrations of PAH in environmental and food samples rely on expensive and laborious methodology. The teachings herein provide methods and kits for detecting the presence or absence of PAH. In certain embodiments, the quantity of PAH in a sample can be determined.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: December 22, 2020
    Assignee: BIOO Scientific Corporation
    Inventors: Nicolas M. Kosa, Joseph F. Krebs, Darshan N. Kasal
  • Patent number: 10775281
    Abstract: Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: September 15, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Christopher Bieniarz, Brian D. Kelly, Jerome W. Kosmeder, II, Eric J. May, Larry Morrison
  • Patent number: 10760108
    Abstract: The present invention relates to a novel variant RNA polymerase sigma factor 70 (?70) polypeptide, a polynucleotide encoding the same, a microorganism containing the polypeptide, and a method for producing L-threonine by using the microorganism.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: September 1, 2020
    Assignee: CJ CHEILJEDANG CORPORATION
    Inventors: Ji Sun Lee, Kwang Ho Lee, Hyo Jin Kim, Keun Chul Lee, Young Bin Hwang
  • Patent number: 10731198
    Abstract: A sensor determines whether a specimen includes a first substance. In an exemplary embodiment, the sensor includes a base. In an exemplary embodiment, the sensor is configured to determine whether the specimen includes the first substance and further includes, on the base, a detection unit on which a second substance is immobilized. The second substance includes a bond that is cleft by the reaction with hydrogen peroxide. In an exemplary embodiment, the specimen that is brought in contact with an enzyme that generates the hydrogen peroxide by the reaction with the first substance is introduced to the detection unit in the sensor configured to determine whether the specimen includes the first substance.
    Type: Grant
    Filed: June 26, 2013
    Date of Patent: August 4, 2020
    Assignees: KYOCERA Corporation, Kyushu University, National University Corporation
    Inventors: Hideharu Kurioka, Shinsuke Sando
  • Patent number: 10696964
    Abstract: Disclosed are methods for identifying bio-molecules with desired properties (or which are most suitable for a round of directed evolution) from complex bio-molecule libraries or sets of such libraries. Some embodiments of the present disclosure provide methods for virtually screening proteins for beneficial properties. Some embodiments of the present disclosure provide methods for virtually screening enzymes for desired activity and/or selectivity for catalytic reactions involving particular substrates. Some embodiments combine screening and directed evolution to design and develop proteins and enzymes having desired properties. Systems and computer program products implementing the methods are also provided.
    Type: Grant
    Filed: September 26, 2014
    Date of Patent: June 30, 2020
    Assignee: Codexis, Inc.
    Inventors: Xiyun Zhang, Russell Javiniar Sarmiento, Donald Scott Baskerville, Gjalt W. Huisman
  • Patent number: 10620210
    Abstract: The present invention provides aptamers that specifically bind to the EGF receptor in a sample, and diagnostic and analytical methods using those aptamers. In some embodiments, the aptamers include a 3? cap. In some embodiments, the 3? cap is an inverted deoxythymidine. In some embodiments the aptamers include a spacer and at least one moiety selected from the group consisting of binding pair member and a detectable label, wherein the spacer is attached to the 5?-end of the aptamer and the moiety is attached the 5? end of the spacer. In some embodiments the spacer is hexaethylene glycol. In some embodiments, the binding pair member biotin. In some embodiments the detectable label is a fluorophore.
    Type: Grant
    Filed: May 5, 2017
    Date of Patent: April 14, 2020
    Assignees: Quest Diagnostics Investments Incorporated, Somalogic, Inc.
    Inventors: Chris Bock, Deborah Ayers, Malti P. Nikrad, Bharat Nathu Gawande, Jennifer C. Bertino, Weimin Sun, Charles M. Strom, Noh Jin Park
  • Patent number: 10486154
    Abstract: A microfluidic device 10 is provided that includes a porous substrate 12 and a plurality of reaction channels 14 disposed on a first side 36 of the porous substrate 12. The reaction channels 14 are defined by a barrier material 16 disposed on the substrate 12 in a user-defined pattern 13. At least one reagent 18 is disposed within each reaction channel 14 in an amount effective to test for the presence of at least one analyte or property in a sample introduced to the device 10.
    Type: Grant
    Filed: April 13, 2015
    Date of Patent: November 26, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: John Benco, Joyce Jordan
  • Patent number: 10458984
    Abstract: A method for evaluation of an interaction between an analyte in a fluid sample and a ligand immobilized on a sensor surface of a biosensor, which comprises the steps of: providing a reference binding curve, representing a reference interaction for a predetermined acquisition cycle, acquiring, using the biosensor, a sample binding curve for the analyte ligand interaction for the predetermined acquisition cycle, registering the deviation of the sample binding curve from the reference binding curve, and classifying the analyte ligand interaction as equivalent to the reference interaction when the registered deviation is less than a predetermined deviation criteria. There is further provided a biosensor system and a computer program.
    Type: Grant
    Filed: January 29, 2015
    Date of Patent: October 29, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventor: Robert Karlsson
  • Patent number: 10330630
    Abstract: Fluid collection devices, analysis instruments and methods for making and using same are disclosed. The fluid collection device is provided with a device and an electrochemical cell. The device has first and second walls defining a microfluidic channel, and a sample application port communicating with the microfluidic channel. The first wall and the second wall are spaced a distance less than 150 microns. The electrochemical cell is disposed on the first wall to contact a sample travelling through the microfluidic channel. The electrochemical cell comprising molecule receptors such that a physical property of the first electrochemical cell is effected upon one or more of the molecule receptors binding to an electroactive species within the sample.
    Type: Grant
    Filed: February 24, 2017
    Date of Patent: June 25, 2019
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Jennifer A. Samproni
  • Patent number: 10302637
    Abstract: A device for detecting target biomolecules is provided. The device consists of an electrically conductive membrane having a biological recognition component configured to bind a target biomolecule immobilized thereon. The membrane is connected to an electric circuit by means of electrodes. A voltage source applies a voltage to the membrane and a resistance monitoring device monitors the resistance of the membrane as a selected volume of fluid sample suspected of containing the target biomolecule is delivered onto the membrane.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: May 28, 2019
    Assignee: Stellenbosch University
    Inventors: Christiaan Gunter Alwyn Viviers, Willem Jacobus Perold, Leon Milner Theodore Dicks, Giles Hubert Coyle Maybery
  • Patent number: 10294507
    Abstract: Disclosed herein is a device that functions as a glucose sensor. The device has a reference electrode; a counter electrode, a working electrode; an electrically conducting membrane; an enzyme layer; a semi-permeable membrane; a first layer of a first hydrogel in operative communication with the working electrode; the first layer of the first hydrogel being operative to store oxygen; wherein the amount of stored oxygen is proportional to the number of freeze-thaw cycles that the hydrogel is subjected to; and a second layer of the second hydrogel. Disclosed too is a method that comprises using periodically biased amperometry towards interrogation of implantable glucose sensors to improve both sensor's sensitivity and linearity while at the same time enable internal calibration against sensor drifts that originate from changes in either electrode activity or membrane permeability as a result of fouling, calcification and/or fibrosis.
    Type: Grant
    Filed: May 27, 2014
    Date of Patent: May 21, 2019
    Assignee: THE UNIVERSITY OF CONNECTICUT
    Inventors: Fotios Papadimitrakopoulos, Santhisagar Vaddiraju
  • Patent number: 10241121
    Abstract: Antioxidant-containing compositions and methods for confirming antioxidant activity of a composition formulated for topical application to skin. Methods for testing a composition for ability to inhibit both ultraviolet radiation-induced lipid peroxidation on skin and ultraviolet radiation-induced reactive oxygen species formation in the stratum corneum. Compositions and methods for treating and preventing photodamage to skin.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: March 26, 2019
    Assignee: BAYER HEALTHCARE LLC
    Inventors: Thomas A. Meyer, Donathan G. Beasley
  • Patent number: 10221401
    Abstract: Compositions and methods are provided for an O2 tolerant Fe—Fe hydrogenase. The hydrogenases of the invention comprise specific amino acid substitutions relative to the native, or wild-type enzymes.
    Type: Grant
    Filed: September 2, 2016
    Date of Patent: March 5, 2019
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: James Robert Swartz, Jamin Koo
  • Patent number: 10202455
    Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: February 12, 2019
    Assignee: IMMUNWORK INC.
    Inventors: Tse-Wen Chang, Hsing-Mao Chu
  • Patent number: 10192010
    Abstract: An example method may include identifying (i) a first group of reactions that corresponds to a first set of precursors and a first set of reaction products and (ii) a second group of reactions that corresponds to a second set of precursors and a second set of reaction products. No precursor in the first set of precursors is also in the second set of precursors, no reaction product in the first set of reaction products is also a precursor in the second set of precursors, and no reaction product in the second set of reaction products is also a precursor in the first set of precursors. The method may also include executing a first processing thread to iteratively calculate respective quantities of the precursors in the first set of precursors and executing a second processing thread to iteratively calculate respective quantities of the precursors in the second set of precursors.
    Type: Grant
    Filed: May 25, 2016
    Date of Patent: January 29, 2019
    Assignee: X Development LLC
    Inventors: Jonathan Ross, Jason Thompson
  • Patent number: 10050214
    Abstract: The present invention relates to a near-ultraviolet stimulated light-emitting compound, and more specifically relates to a fluorescent quinazolinone compound which is stimulated in the near ultraviolet region. The fluorescent quinazolinone compound according to the present invention is colorless in daylight but exhibits a high fluorescent intensity when irradiated with light in the near ultraviolet region. Also, the fluorescent quinazolinone compound can be easily used with printed and plastic molded products since the compound not only has a high degree of light fastness and thermal stability but also high dispersibility.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: August 14, 2018
    Assignees: NANO CMS CO., LTD., KOREA RESEARCH INSTITUTE OF CHEMICAL TECHNOLOGY
    Inventors: Je Young Park, In Ja Lee, Shi Surk Kim, Kun Jun, Seung Rim Shin, Kyoung Lyong An
  • Patent number: 10031119
    Abstract: Colorimetric explosive detection kits comprising compact and flexible packaging with an explosive collection assembly and detection reagents disposed thereon in a single-easy-to use system.
    Type: Grant
    Filed: November 5, 2015
    Date of Patent: July 24, 2018
    Assignee: The United States of America as Represented by the Secretary of the Army
    Inventors: Christopher D. Applegate, Joseph M. Laquidara
  • Patent number: 10023900
    Abstract: The invention relates to method for quantification of the absolute amount of allergen in an allergen sample comprising: a) providing a known amount of one or more allergen calibration standard peptide(s) having a sequence of amino acids which is identical with, and optionally unique for, a sequence to be found in the allergen to be quantified and optionally labelling said allergen calibration standard peptide(s), b) degrading the allergen sample to obtain a mixture of peptides, and optionally labelling said peptides with one or more labelling agent(s), wherein at least the peptides in the degraded allergen sample or the calibration standard peptides are labelled, and if both the peptides in the degraded allergen sample and the allergen calibration standard peptide(s) are labelled, the labelling agent(s) used for labelling the allergen calibration standard peptide(s) are different from the labelling agent(s) used for labelling the peptides of the degraded allergen sample, c) quantifying the absolute amount
    Type: Grant
    Filed: March 5, 2014
    Date of Patent: July 17, 2018
    Assignee: ALK-ABELLO A/S
    Inventor: Ulla Seppälä
  • Patent number: 10011842
    Abstract: In this invention, cell lines are created for enzyme inhibitory testing of inhibitors against Plasmodium falciparum DHFR-TS and HPPK-DHPS. Provided the complementing DHFR-TS and HPPK-DHPS have sufficient activities to support growth of the surrogates in un-supplemented medium, the same surrogates could be used for screening inhibitors of targets against other parasite and pathogen species e.g. Plasmodium vivax, Trypanosoma brucei, Trypanosoma cruzi, Toxoplasma gondii or Mycobacterium tuberculosis. The cell lines in this invention are Escherichia coli strain whose thyA, folA, folK, and folP genes were disrupted using genetic knockout coupled with elimination of antibiotic resistance markers. The thyA KO, folP KO, folK KO, thyAfolA KO, folKfolP KO, thyAfolAfolP KO, thyAfolAfolK KO and thyAfolAfolKfolP KO E.
    Type: Grant
    Filed: September 18, 2014
    Date of Patent: July 3, 2018
    Assignee: National Science and Technology Development Agency
    Inventors: Nattida Suwanakitti, Yuwadee Talawanich, Yongyuth Yuthavong, Sumalee Kamchonwongpaisan
  • Patent number: 9984278
    Abstract: The present invention generally relates to non-linear imaging systems and methods for use in various assisted reproductive technologies and other applications. In one aspect, the present invention is generally directed to two-photon and other non-linear microscope techniques that can be used to determine the status or maturity state of eggs or egg follicles, for example by determining the presence of polar bodies within the egg follicles. In some embodiments, endogenous metabolites, such as NADH or FAD, can be determined, and in some cases without externally altering those metabolite concentrations or without adding other compounds. Other embodiments of the invention are also directed to computer programs or systems that can be used to determine the status or maturity state of eggs or egg follicles, kits in such systems, or the like.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: May 29, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Daniel Needleman, Manqi Deng, Timothy H. Sanchez
  • Patent number: 9975951
    Abstract: The present disclosure provides various molecular constructs having a targeting element and an effector element. Methods for treating various diseases using such molecular constructs are also disclosed.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: May 22, 2018
    Inventors: Tse-Wen Chang, Hsing-Mao Chu
  • Patent number: 9834578
    Abstract: The present disclosure provides methods and composition involving increased sensitivity of compounds using mass spectrometry. In one embodiment, a method of increasing the sensitivity for detection of a carbonyl group-containing compound by mass spectrometry can comprise derivatizing the carbonyl group-containing compound with an O-substituted hydroxylamine thereby producing an oxime, resulting in enhanced sensitivity of detection by mass spectrometry, as compared to the underivatized carbonyl group-containing compound. Additionally, a method for assaying a carbonyl group-containing compound can comprise reacting the carbonyl group-containing compound in a sample with an O-substituted hydroxylamine to produce an oxime and performing analysis with mass spectrometric detection of the oxime by a mass spectrometry instrument.
    Type: Grant
    Filed: May 24, 2011
    Date of Patent: December 5, 2017
    Assignee: University of Utah Research Foundation
    Inventors: Bingfang Yue, Alan L. Rockwood, Mark M. Kushnir
  • Patent number: 9816127
    Abstract: The present invention provides an assay for detection of oxidized glutathione (GSSG).
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: November 14, 2017
    Assignee: PROMEGA CORPORATION
    Inventors: Fen Huang, Dieter Klaubert, John Shultz, Wenhui Zhou
  • Patent number: 9795962
    Abstract: The invention is to devices and methods for rapid determination of analytes in liquid samples. The devices and methods incorporate a sample dilution feature and multiple immunosensors for performing a ratiometric immunoassay on a first analyte and a second analyte, for example, hemoglobin and hemoglobin A1c or albumin and glycosylated albumin. The devices are preferably capable of being used in the point-of-care diagnostic field.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: October 24, 2017
    Assignee: Abbott Point of Care Inc.
    Inventor: Cary James Miller
  • Patent number: 9730935
    Abstract: Methods and compositions are provided for treating an individual having cancer. Aspects of the methods include administering to the individual an inhibitor of a non-canonical Notch signaling pathway gene in an amount effective to treat the cancer. Also provided are reagents, devices and kits thereof that find use in practicing the subject methods.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: August 15, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Bingwei Lu, Kyu-Sun Lee
  • Patent number: 9671348
    Abstract: The present invention provides a method for measuring a component to be measured in a sample, comprising converting the component to be measured in the sample into hydrogen peroxide and measuring the formed hydrogen peroxide in the presence of an ?-keto acid using an oxidative-coloring chromogen. The present invention also provides a method for suppressing the influence of a peroxide on a method of converting a component to be measured in a sample into hydrogen peroxide and measuring the formed hydrogen peroxide using an oxidative-coloring chromogen, the suppression method comprising using an ?-keto acid. The measuring method and the suppression method of the present invention using an ?-keto acid suppress the influence of a peroxide to give an accurate measurement of the component to be measured in the sample.
    Type: Grant
    Filed: December 12, 2011
    Date of Patent: June 6, 2017
    Assignee: KYOWA MEDEX CO., LTD.
    Inventor: Tomomi Murakami
  • Patent number: 9642922
    Abstract: Provided is an activatable probe that undergoes intramolecular cyclization and subsequent aggregation in apoptotic tumor cells upon peptidase-initiated, and most advantageously caspase-3, activation. These caspase-sensitive nano-aggregation probes (C-SNAFs) are generally biocompatible, possess NIR spectral properties or may serve as PET or MRI imaging agents, and have a mechanism of target-mediated nanostructure self-assembly amenable to in vivo use. The probes encompass biocompatible condensation chemistry products that comprise D-cysteine and 2-cyano-6-hydroxyquinoline (CHQ) moieties linked to an amino-luciferin scaffold, and which can be activated by a two-step reaction requiring caspase-3/7-mediated cleavage of an aspartate-glutamate-valine-aspartate (L-DEVD) capping peptide and the free intracellular thiol-mediated reduction of the disulfide bond.
    Type: Grant
    Filed: August 20, 2014
    Date of Patent: May 9, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Jianghong Rao, Deju Ye, Adam Shuhendler, Frederick Te-Ning Chin, Jongho Jeon, Bin Shen
  • Patent number: 9550979
    Abstract: The present invention relates to mutant alcohol dehydrogenase enzymes, microorganisms comprising the mutant alcohol dehydrogenase enzymes, and methods for the production of products by microbial fermentation using the microorganisms.
    Type: Grant
    Filed: April 5, 2013
    Date of Patent: January 24, 2017
    Assignee: Lanza Tech New Zealand Limited
    Inventors: Michael Koepke, Wayne Michael Patrick, Danielle Joan Maddock, Monica Gerth
  • Patent number: 9528990
    Abstract: The invention relates to fluorescent/fluorogenic probes of formula (I?) or (II?): wherein Z is chosen between: —NH2 and —OH, or in the fluorescence quencher group consisting of: —NO2; —N?N—R1; R1 being any organic group that does not obscure the corresponding azo bond; —NHCO-Pept.; Pept. being a peptide residue or any organic group that does not obscure the corresponding amide bond; —O-Glyc.; Glyc. being a oligoglycoside residue that does not obscure the corresponding glycosidic bond; —O—C(O)—R2; —O—P(O)(OR2)(OR2?) and —O—S(O)2—R2; R2 and R2? being independently a hydrogen atom or an organic group that does not obscure the corresponding ester bond; and ?Ra, Rb, Rc, Rd and Re being independently a hydrogen atom or any organic group that does not obscure the corresponding arylether bond so as not to prevent its possible cleavage by a myeloperoxidase activity.
    Type: Grant
    Filed: May 22, 2015
    Date of Patent: December 27, 2016
    Assignees: BIOMERIEUX S.A., CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE ROUEN
    Inventors: Sylvain Orenga, Valérie Chalansonnet, Arnaud Chevalier, Pierre-Yves Renard, Anthony Romieu, Benoit Roubinet
  • Patent number: 9506939
    Abstract: Provided herein are assay control materials comprising stable analytes and lyophilized unstable analytes, and methods of making and using the same.
    Type: Grant
    Filed: May 6, 2014
    Date of Patent: November 29, 2016
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Alireza Ebrahim, Karl De Vore, Christopher Spates
  • Patent number: 9458491
    Abstract: The present invention provides a method for measuring the concentration of an analyte in a test solution wherein the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1 and an enzyme that catalyzes a reaction represented by Reaction Formula 2 to act on a test solution containing mevalonic acid and/or 3 -hydroxymethylglutaryl coenzyme A in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same.
    Type: Grant
    Filed: January 19, 2010
    Date of Patent: October 4, 2016
    Assignee: ASAHI KASEI PHARMA CORPORATION
    Inventor: Takeshi Matsuoka
  • Patent number: 9394562
    Abstract: The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.
    Type: Grant
    Filed: January 2, 2015
    Date of Patent: July 19, 2016
    Assignees: AMOREPACIFIC CORPORATION, SNU R&DB FOUNDATION
    Inventors: Jun Seong Park, Duck Hee Kim, Han Kon Kim, Eun Mi Kim, Byung Gee Kim
  • Patent number: 9366645
    Abstract: Embodiments of the present invention provide binding molecule-functionalized high electron mobility transistors (HEMTs) that can be used to detect toxins, pathogens and other biological materials. In a specific embodiment, an antibody-functionalized HEMT can be used to detect botulinum toxin. The antibody can be anchored to a gold-layered gate area of the HEMT through immobilized thioglycolic acid. Embodiments of the subject detectors can be used in field-deployable electronic biological applications based on AlGaN/GaN HEMTs.
    Type: Grant
    Filed: January 7, 2014
    Date of Patent: June 14, 2016
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Fan Ren, Stephen John Pearton, Tanmay P. Lele
  • Patent number: 9327230
    Abstract: A gas cleaning system including a wet scrubber (14) for removal of sulphur dioxide from a gas and an oxidation basin system (42) for receiving effluent generated in the removal of sulphur dioxide from the process gas. The oxidation basin system (42) comprises an oxidation basin (43) for containing the effluent during treatment thereof, an oxygen supply system (47) for supplying oxygen to the effluent in the oxidation basin (43), and a contacting system for bringing the effluent into contact with an enzyme which catalyzes the oxidation of bisulphite and/or sulphite ions.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: May 3, 2016
    Assignee: ALSTOM Technology Ltd
    Inventors: Fredrik Jens Brogaard, Mikael Larsson
  • Patent number: 9302227
    Abstract: A membrane separation assembly that includes an integrated filter element and at least one membrane module housed within a first vessel and a second vessel containing at least one membrane module, which is stacked or aligned adjacent to the first vessel. The first vessel is configured to allow liquids to be trapped and removed from the assembly, and gases to flow to and through the membrane modules of the first vessel and the membrane modules of the second vessel, which are ultimately withdrawn from the assembly. The assembly is useful in the conditioning of fuel gas to separate methane from C2+ hydrocarbons.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: April 5, 2016
    Assignee: Membrane Technology and Research, Inc.
    Inventor: Kaaeid A. Lokhandwala
  • Patent number: 9284596
    Abstract: Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
    Type: Grant
    Filed: February 13, 2014
    Date of Patent: March 15, 2016
    Assignee: Battelle Energy Alliance, LLC
    Inventors: David Neil Thompson, Emily DeCrescenzo Henriksen, David William Reed, Jill Renee Jensen
  • Patent number: 9284599
    Abstract: A method of testing for the presence of a preselected target nucleic acid, protein or antigen in a biological sample by exposing nucleic acids, proteins or antigens to a probe having a catalytic element and binding element. The catalytic element catalyses at least one reaction that results in a physical change such that identifiable elements provide an indication of the presence of the target.
    Type: Grant
    Filed: March 4, 2011
    Date of Patent: March 15, 2016
    Assignee: Eluceda Limited
    Inventors: Ian Eastwood, Andrew Reid, Mark Saw
  • Patent number: RE47607
    Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.
    Type: Grant
    Filed: October 13, 2016
    Date of Patent: September 17, 2019
    Assignee: GENE STREAM PTY LTD.
    Inventors: Marco Peter Leu, John Michael Daly