Involving Peroxidase Patents (Class 435/28)
  • Patent number: 10823698
    Abstract: The invention relates to, among other things, a method for performing electrophoresis with in-situ calibration. The method includes combining a volume of a test sample with a volume or quantity of a calibrating sample to form a final volume, where the volume of the calibrating sample includes a known concentration of a calibrator and the final volume includes a known ratio of test sample to calibrating sample. The method also includes depositing a loading fraction in a receiving well of an electrophoretic gel, in which the loading fraction is a fraction of the final volume, and separating the loading fraction along a common separation lane of the electrophoretic gel such that components of the test sample and the calibrator are separated from one another along the common separation lane.
    Type: Grant
    Filed: March 27, 2015
    Date of Patent: November 3, 2020
    Assignee: Helena Laboratories Corporation
    Inventors: Philip Guadagno, Erin Summers
  • Patent number: 10794918
    Abstract: The present invention relates to the use of glial fibrillary acidic protein (GFAP) as a diagnostic marker for intracerebral hemorrhage. The invention especially relates to methods for the early detection of intracerebral hemorrhage. Such early and rapid detection can be performed rapidly e.g. by a test strip format assay. GFAP can be used as a stand-alone marker or in combination with one or more other markers.
    Type: Grant
    Filed: May 13, 2015
    Date of Patent: October 6, 2020
    Inventors: Matthias Sitzer, Christian Foerch
  • Patent number: 10675623
    Abstract: A method includes conveying a detection solution containing a target amplicon into a detection module of a molecular diagnostic test device. The detection module includes a detection surface including a series of capture probes to which a first portion of the target amplicon is bound when the detection solution is conveyed. A first reagent formulated to produce a visible signal indicating a presence of the target amplicon is then conveyed into the detection module. The first reagent is bound to a second portion of the target amplicon when the first reagent is conveyed. A second reagent is conveyed into the detection module. The second reagent includes a precipitating substrate formulated to catalyze the production of the visible signal by producing an insoluble colored product when the second reagent is in contact with the first reagent. The method includes viewing the visible signal via a transparent portion of the detection module.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: June 9, 2020
    Assignee: Visby Medical, Inc.
    Inventors: Boris Andreyev, Rajinder K. Bhatia, Victor Briones, Phoebe Cao, Jesus Ching, Brian Ciopyk, Adam De La Zerda, Jonathan H. Hong, Helen Huang, Colin Kelly, Adrienne C. Lam, Gregory Loney, Danielle McSheery, Keith E. Moravick, Valeria Revilla, Shaunak Roy, David D. Swenson
  • Patent number: 10583420
    Abstract: A process for manufacturing particles of water-absorbing material is provided. The process includes providing a powder bed composed of an absorptive powder comprising a water-absorbing polysaccharide onto a surface; releasing an aqueous solution from a solution dispenser so as to contact the powder bed, 5 thereby forming a solution-impregnated humid material; letting the solution-impregnated humid material agglomerate in substantially shear-less conditions to form an agglomerated humid material, the solution-impregnated humid material being supported by the surface; and drying the agglomerated humid material, thereby forming the particles.
    Type: Grant
    Filed: September 30, 2015
    Date of Patent: March 10, 2020
    Assignee: 7905122 CANADA INC.
    Inventors: Paul Jollez, Isabelle Bolduc
  • Patent number: 10551377
    Abstract: A method of quantifying an autoantibody includes the steps of reacting a biological sample containing the autoantibody as a test substance with an antigen that is specifically recognized by and bound to the autoantibody, in competition with a known amount of a competition antibody that competes with the autoantibody for binding to the antigen; reacting the autoantibody bound to the antigen with a detection antibody that recognizes and binds to the autoantibody, but does not recognize the competition antibody; measuring a signal derived from the detection antibody bound to the autoantibody; and calculating an amount of the autoantibody contained in the biological sample by utilizing, as an index therefor, an amount of the competition antibody that provides 50% reduction of the signal derived from the detection antibody which is bound to the autoantibody in absence of the competition antibody.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: February 4, 2020
    Assignee: MagArray, Inc.
    Inventor: Hideyuki Iwata
  • Patent number: 10254288
    Abstract: A method for species-independent measurement of complement (C) activation in animals. The method comprises taking samples in the range of 3-100 microliter of anticoagulated blood, plasma or serum of an animal (specimen), mixing the specimen with a specificity converting protein matrix (SCM), mixing to the specimen/SCM mixture an activator of the C system (Act), incubating the specimen/SCM/Act mixture at a temperature between 36° C. to 38° C. for a time of 5-120 min and determining the production of one or more human proteins by ELISA or other analytical methods.
    Type: Grant
    Filed: August 16, 2013
    Date of Patent: April 9, 2019
    Assignee: Quidel Corporation
    Inventors: Marieluise Wippermann, Janos Szebeni
  • Patent number: 10001492
    Abstract: The present invention relates to a method for diagnosing inflammatory bowel disease (IBD), the method comprising determining the concentration of at least one IBD-specific biomarker in a sample of the colonic mucocellular layer obtained from a subject.
    Type: Grant
    Filed: November 7, 2013
    Date of Patent: June 19, 2018
    Assignee: Diagnodus Limited
    Inventors: Alexandre Loktionov, Tatiana Bandaletova
  • Patent number: 9980668
    Abstract: A composition for sensing an analyte can include a photoluminescent nanostructure complexed to a sensing polymer, where the sensing polymer includes an organic polymer non-covalently bound to the photoluminescent nanostructure and an analyte-binding protein covalently bound to the organic polymer, and where the analyte-binding protein is capable of selectively binding the analyte, and the analyte-binding protein undergoes a substantial conformational change when binding the analyte. Separately, a composition for sensing an analyte, can include a complex, where the complex includes a photoluminescent nanostructure in an aqueous surfactant dispersion and a boronic acid capable of selectively reacting with an analyte. The compositions can be used in devices and methods for sensing an analyte.
    Type: Grant
    Filed: July 31, 2012
    Date of Patent: May 29, 2018
    Assignee: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
    Inventors: Michael S. Strano, Paul W. Barone, Jin-Ho Ahn, Kyungsuk Yum, Thomas P. McNicholas
  • Patent number: 9772330
    Abstract: The present invention concerns methods for the detection f target molecules in a sample including several steps of signal amplification allowing the detection of a very low number of target molecules in the tested sample. The detection assay is based on the use of a universal probe which enables the signal amplification. The specific recognition of the target molecule is achieved by using a specific binding agent, preferably an aptamer. The invention further concerns kits and methods for the diagnosis of pathological conditions.
    Type: Grant
    Filed: December 10, 2014
    Date of Patent: September 26, 2017
    Assignee: APTATECK BIO LTD.
    Inventor: Ofer Nussbaum
  • Patent number: 9737890
    Abstract: The present invention provides a microfluidic device which comprises a drive module and a microfluidic platform. The drive module further comprises a rotary unit and a vibration unit for driving the microfluidic platform, and the microfluidic platform further comprises multiple microfluidic elements for performing tests. The present invention also provides a method for operating a microfluidic device. The method comprises steps using the rotary unit and steps using the vibration unit to distribute sample in a microfluidic structure.
    Type: Grant
    Filed: November 30, 2014
    Date of Patent: August 22, 2017
    Assignee: SHAOXING PUSHKANG BIOTECHNOLOGY CO., LTD.
    Inventors: Chia-Hui Lin, Bo Yu, Yi-Feng Yang
  • Patent number: 9663815
    Abstract: An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: May 30, 2017
    Assignee: Massachusetts Institute of Technology
    Inventors: Alice Y. Ting, Jeffrey Daniel Martell
  • Patent number: 9651546
    Abstract: The invention provides peptide compositions and mixtures useful for the detection of antibodies that bind to Ehrlichia antigens. The peptide compositions and mixtures comprise polypeptide sequences based on an immunogenic fragment of the Ehrlichia Outer Membrane Protein 1 (OMP-1) protein. The invention also provides devices, methods, and kits comprising such peptide compositions and mixtures useful for the detection of antibodies that bind to Ehrlichia antigens and the diagnosis of monocytic and/or granulocytic ehrlichiosis.
    Type: Grant
    Filed: October 11, 2013
    Date of Patent: May 16, 2017
    Assignee: ABAXIS, INC.
    Inventors: Rajesh K. Mehra, Kenneth P. Aron, Dennis M. Bleile, Timothy P. Forsyth, Jeremy D. Walker, Cristina R. Cuesico
  • Patent number: 9495514
    Abstract: A multi-variable statistical predictive leading-indicator approach is employed for identifying newborns at risk of clinically significant hyperbilirubinemia and for determining to administer interventions to at-risk newborns. In embodiments, a multi-variable logistic regression statistical model capable of calculating a probability of clinically significant hyperbilirubinemia is generated. Using an input data set for a newborn and the multi-variable logistic regression statistical model, a probability of clinically significant hyperbilirubinemia is determined for the newborn and presented to a clinician.
    Type: Grant
    Filed: December 23, 2009
    Date of Patent: November 15, 2016
    Assignee: CERNER INNOVATION, INC.
    Inventor: Douglas S. McNair
  • Patent number: 9399657
    Abstract: The present invention relates to chemiluminescent method and regent to detect analyte. One aspect of the current invention relates to using enzyme substrate that can be cleaved by target enzyme to release chemiluminescent compound giving light signal for the detection of varieties of target enzymes. Another aspect of the current invention relates to use chemiluminescent enzyme coupled with analyte binding molecules to detect specific analyte molecules in a homogenous phase.
    Type: Grant
    Filed: October 15, 2008
    Date of Patent: July 26, 2016
    Inventors: Tianxin Wang, Xingxiang Li
  • Patent number: 9354241
    Abstract: The present application provides compositions and methods useful for detecting and monitoring acquired aplastic anemia.
    Type: Grant
    Filed: October 8, 2011
    Date of Patent: May 31, 2016
    Assignee: SHANGHAI KEXIN BIOTECH CO., LTD.
    Inventors: Yue Zhang, Jun Bao, Hua Mao, Fei Xiang, Hongbin Sun, Jie Qian, Yongjun Han, Weina Situ
  • Patent number: 9310387
    Abstract: A test apparatus, which may compare detection data of a detector with reference data and correct a positional error in the detector due to the failing of a motor, and a method of controlling the same are provided. The test apparatus includes a detector configured to irradiate light to a plurality of chambers of a reaction device and detect a detection target, a motor configured to move the detector such that light is irradiated to the plurality of chambers, and a controller configured to compare detection data of the detector regarding the reaction device with reference data, determine a positional error in the detector, and correct the positional error.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: April 12, 2016
    Assignee: SAMSUNG ELECTRONICS CO., LTD.
    Inventors: Se Do Gwon, Kyu Tae Kang
  • Patent number: 9291515
    Abstract: The invention discloses a rapid test device, includes a cup vessel (4), which comprises a first chamber (41) and a dosing piston assembling hole (44) communicating with the first chamber (41); a base (8) fastened at the bottom of the cup vessel (4); a second chamber (81), formed between the base (8) and the cup vessel (4) and communicating with the dosing piston assembling hole (44); a partition (7), arranged in the second chamber (81) and provided with test paper; a dosing piston (6), provided with a dosing slot (61) thereon and provided with a key hole at one end, inserted in the dosing piston assembling hole (44); and a key (1) matching the key hole. In present invention the sample in the dosing slot can be transmitted into the second chamber by rotating the dosing piston. The sample is absorbed by the test paper after flowing by the test paper, and test result appears on the test paper.
    Type: Grant
    Filed: April 22, 2011
    Date of Patent: March 22, 2016
    Assignee: ASSURE TECH. (HANGZHOU) CO., LTD.
    Inventors: Shisheng Ling, Xuehao Ji, Keyuan Yao
  • Patent number: 9091691
    Abstract: The present invention relates to immunochemical visualization and quantification of single target entities, such as single molecules, single molecular structures, single particles, etc. in samples wherein said single entities are immobilized. In particular, the invention relates to methods for visualization and quantification of single units of biological or chemical targets, in particular to immunochemical visualization of single molecules of biological targets in histological samples. The methods of the invention comprise a step of forming discrete deposits of detectable molecules at single target sites of sample mediated by an enzyme with oxydoreductase activity, wherein a single target site comprises a single unit of a target. The invention also relates to assays comprising the present visualization and quantification methods and diagnostic applications of said methods.
    Type: Grant
    Filed: October 15, 2010
    Date of Patent: July 28, 2015
    Assignee: Dako Denmark A/S
    Inventor: Jesper Lohse
  • Patent number: 9029092
    Abstract: Methods, reagents, kits and systems are disclosed for determining an analyte in a sample suspected of containing the analyte where all reagents are soluble in aqueous solution. One assay method includes treating a sample suspected of containing the analyte under conditions such that if the analyte is present, an activator is brought into reactive configuration with a chemiluminescent compound to activates it. The sample is also treated with an agent to reduce signal not related to analyte. Finally, the sample is treated with a trigger solution thereby producing light from the activated chemiluminescent compound. No reagents are associated with a surface or other solid phase.
    Type: Grant
    Filed: February 26, 2010
    Date of Patent: May 12, 2015
    Assignee: Beckman Coulter, Inc.
    Inventors: Hashem Akhavan-Tafti, Dean G. Binger, Ying Chen, Renuka De Silva, Terri McLernon, James Mendoza, Bruce H. Odegaard, Michael Salvati, Nir Shapir, Wenhua Xie
  • Publication number: 20150111228
    Abstract: The present disclosure provides an optical biosensor including a complex including a porous support and an enzyme supported inside the pores of the porous support and a method for preparing the same. Since a dye does not flow but is concentrated in one place, the optical biosensor of the present disclosure has remarkably improved sensitivity. In addition, it is possible to carry out quantitative determination and qualitative analysis since color intensity increases with time.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 23, 2015
    Applicants: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION, GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Jungbae Kim, Min-Gon Kim, Hyou-Arm Joung, Yongho Wee
  • Patent number: 9012170
    Abstract: An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.
    Type: Grant
    Filed: January 16, 2014
    Date of Patent: April 21, 2015
    Assignee: Massachusetts Institute of Technology
    Inventors: Alice Y. Ting, Jeffrey Daniel Martell
  • Publication number: 20150104819
    Abstract: The present invention is directed to methods of diagnosing the presence or absence of a bacterial infection in a patient, in particular, a tuberculosis infection, by measuring exhaled, isotopically labeled nitrogen gas after administration of isotopic ally labeled isoniazid.
    Type: Application
    Filed: March 7, 2013
    Publication date: April 16, 2015
    Inventors: Graham Timmins, Seong Choi, Zachary Sharp, Viorel Atudorei, Mamoudou Maiga, William Bishai
  • Patent number: 8993313
    Abstract: Provided are an analytical instrument and an analytical method that allow for the direct analysis of a target substance in an undiluted specimen by a transmission photometry in a tightly closed cell container space. An analytical instrument is an analytical instrument for analyzing a target substance contained in a specimen flown in the tightly closed cell container by utilizing an oxidative color-developing agent and an oxidative enzyme reaction, in which an upper substrate and a lower substrate are arranged facing each other and at least a part of the upper substrate and/or at least a part of the lower substrate are/is made of a material transmitting light used for the analysis and having oxygen transmission properties.
    Type: Grant
    Filed: October 12, 2012
    Date of Patent: March 31, 2015
    Assignee: ARKRAY, Inc.
    Inventor: Toshihiro Imai
  • Publication number: 20150079620
    Abstract: Provided is a measuring method of a component to be measured in a specimen while suppressing an influence of bilirubin. A measuring method of a component to be measured, comprising converting the component to be measured in the specimen to hydrogen peroxide through an enzymatic reaction, reacting the formed hydrogen peroxide with an oxidative-coloring chromogen in the presence of a peroxidase, and measuring an absorbance of the colored reaction solution to thereby determine the component to be measured, wherein a fatty acid or a salt thereof coexists. The measuring method of a component to be measured in a specimen according to the present invention is useful in clinical diagnosis.
    Type: Application
    Filed: April 18, 2013
    Publication date: March 19, 2015
    Inventors: Hideyuki Kuwata, Tomoko Aratake, Kenta Kinjo
  • Publication number: 20150079583
    Abstract: One aspect of the present disclosure relates to a calorimeter for detecting the presence of a target analyte in a fluid sample. The calorimeter can include a substrate, a hermetically-sealed, thermally decoupled central reaction zone associated with the substrate, at least one droplet transport region, and detection electronics. The at least one droplet transport region can be associated with the substrate and configured to merge a reagent droplet with a sample droplet including the fluid sample to form a reaction droplet in the central reaction zone. The detection electronics can be in electrical and/or thermal communication with the central reaction zone and associated with the substrate. The calorimeter can be configured to detect a heat of reaction produced by a reaction event between the target analyte and a capture reagent upon formation of the reaction droplet.
    Type: Application
    Filed: August 22, 2014
    Publication date: March 19, 2015
    Inventors: Franz Baudenbacher, Raymond Mernaugh, John Mayo, Brad Lubbers
  • Patent number: 8980241
    Abstract: A method of covalently bonding a cyclic nitroxide radical compound to a hydrophobic block of a specific hydrophylic-phobic block copolymer, and polymerized cyclic nitroxide radical compound copolymerized in this manner, as well as use of such a compound, for instance, in the medical field are provided. The compound demonstrates long term stability in vivo under reductive environment.
    Type: Grant
    Filed: December 4, 2008
    Date of Patent: March 17, 2015
    Assignee: University of Tsukuba
    Inventors: Yukio Nagasaki, Toru Yoshitomi, Hirofumi Matsui, Aki Hirayama, Takashi Mamiya, Akira Matsumura, Kensuke Suzuki, Hideo Tsurushima, Aiki Marushima, Kazuko Toh, Daisuke Miyamoto
  • Patent number: 8980544
    Abstract: A boronic or borinic acid compound is used to inhibit the activity of a sulfenic acid-containing protein. Thus, a biologically-active sulfenic acid-containing protein is contacted with an activity-inhibiting effective amount of a boronic or borinic acid compound of Formula I or a salt, hydrate or solvate thereof, whose components are disclosed within, and that contact is maintained for a time sufficient to inhibit the biological activity of the protein.
    Type: Grant
    Filed: August 27, 2013
    Date of Patent: March 17, 2015
    Assignee: The Penn State Research Foundation
    Inventors: Stephen Benkovic, Chunyu Liu, John W. Tomsho
  • Publication number: 20150072367
    Abstract: Provided is a method for measuring a substance in a blood sample, which allows avoidance of the influences of both of bilirubin and hemoglobin by simple operations. Provided is a method for measuring a substance in a blood sample by an enzymatic method using an oxidizable color reagent, the method including (1) bringing the blood sample into contact with a non-ionic surfactant; and then (2) bringing the resultant sample into contact with a betaine-type amphoteric surfactant, to perform an enzyme reaction and a color reaction by an oxidizable color reagent at the same time as the contact or after the contact.
    Type: Application
    Filed: April 1, 2013
    Publication date: March 12, 2015
    Applicant: SEKISUI MEDICAL CO., LTD.
    Inventor: Toyomi Yamaguchi
  • Publication number: 20150064192
    Abstract: Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.
    Type: Application
    Filed: November 14, 2014
    Publication date: March 5, 2015
    Inventors: DANIEL M. GORMAN, TROY D. RANDALL, ALBERT ZLOTNIK
  • Publication number: 20150037829
    Abstract: An imaging method comprising expressing in cells a Class I heme peroxidase, which optionally is fused with a protein of interest or a cellular localization signal peptide, and contacting the cells with a substrate of the Class I heme peroxidase to allow conversion of the substrate into a product via an oxidation reaction catalyzed by the Class I heme peroxidase, wherein the product releases a signal detectable by a microscope such as an electron microscope. Also disclosed herein are monomeric mutants of a Class I heme peroxidase and mutants of the enzyme that exhibit elevated enzymatic activity as compared to the corresponding wild-type counterpart.
    Type: Application
    Filed: September 14, 2012
    Publication date: February 5, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Alice Y. Ting, Jeffrey Daniel Martell
  • Patent number: 8927226
    Abstract: The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: January 6, 2015
    Assignee: SurModics, Inc.
    Inventors: Gary Opperman, Wendy Nelson
  • Publication number: 20140363837
    Abstract: The present invention relates to recombinant heme-containing horseradish peroxidase isoenzymes with improved properties. In particular, the present invention relates to a plant enzyme kit comprising recombinant peroxidase isoenzymes, preferably horseradish peroxidase isoenzymes.
    Type: Application
    Filed: October 19, 2012
    Publication date: December 11, 2014
    Applicant: Technische Universitaet Graz (Geaz University of Technology
    Inventors: Florian Krainer, Laura Naeaetsaari, Anton Glieder, Martin Kulterer, Victoria Reichel
  • Publication number: 20140363817
    Abstract: The present invention concerns a method for labeling specifically living bacteria of a given category in a sample, the method including: a) incubating said bacteria of said sample with at least one analog of a monosaccharide compound, and b) contacting the bacteria with a labeling molecule having a second reactive group.
    Type: Application
    Filed: January 16, 2013
    Publication date: December 11, 2014
    Inventors: Sam Dukan, Audrey Dumont, Monzer Awwad, Annie Malleron, Boris Vauzeilles
  • Patent number: 8900825
    Abstract: The invention provides novel immunogenic proteins LigA and LigB from Leptospira for use in the development of effective vaccines and antibodies, as well as improved diagnostic methods and kits.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: December 2, 2014
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Yung-Fu Chang, Raghavan U. M. Palaniappan
  • Publication number: 20140349870
    Abstract: A chemo-mechano-chemical (C1-M-C2) system includes a base supporting an actuatable structure, said structure comprising a functionalized portion and being embedded in an environmentally responsive gel capable of volume change in response to an environmental stimulus; a first fluid layer disposed over the base and in contact with the actuatable structure, said first fluid layer comprising the environmentally responsive gel; and a second fluid layer in contact with the actuatable structure, wherein the layers are positioned such that the functionalized portion is in contact with the second layer in a first relaxed state and in contact with the first layer in a second actuated state and wherein the functionalized portion interacts with at least one of the layers to provide a chemical or physical response.
    Type: Application
    Filed: May 2, 2014
    Publication date: November 27, 2014
    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Joanna AIZENBERG, Ximin HE, Michael AIZENBERG
  • Publication number: 20140335505
    Abstract: Systems and methods are provided for collecting, preparing, and/or analyzing a biological sample. A sample collection site may be utilized with one or more sample processing device. The sample processing device may be configured to accept a sample from a subject. The sample processing device may perform one or more sample preparation step and/or chemical reaction involving the sample. Data related to the sample may be sent from the device to a laboratory. The laboratory may be a certified laboratory that may generate a report that is transmitted to a health care professional. The health care professional may rely on the report for diagnosing, treating, and/or preventing a disease in the subject.
    Type: Application
    Filed: July 18, 2014
    Publication date: November 13, 2014
    Inventor: Elizabeth A. Holmes
  • Publication number: 20140335553
    Abstract: Methods are provided measuring L-lysine using a variant enzyme, an L-lysine measurement kit, and an enzyme sensor. Variant L-amino acid oxidase having a predetermined amino acid mutation, and having oxidase activity that is highly substrate-specific for L-lysine; a method for measuring L-lysine using this variant enzyme; an L-lysine measurement kit; and an enzyme sensor are also provided.
    Type: Application
    Filed: July 29, 2014
    Publication date: November 13, 2014
    Applicants: AJINOMOTO CO., INC., TOYAMA PREFECTURE
    Inventors: Yasuhisa Asano, Daisuke Matsui
  • Patent number: 8883436
    Abstract: Enzymes and/or polypeptides and/or mixtures of interest are evaluated during hydrolysis of cellulosic material by the use of indicator constituents such as fluorescent agents, resulting in efficient high-throughput analysis of enzymes and/or polypeptides. A high-throughput assay for the analysis of inter alia, pretreated corn stover (PCS) hydrolysis is also disclosed.
    Type: Grant
    Filed: July 13, 2010
    Date of Patent: November 11, 2014
    Assignee: Novozymes A/S
    Inventors: Marco Malten, Keith McFarland
  • Patent number: 8883439
    Abstract: The invention is a method for determining the concentration of an analyte in whole blood, wherein the analyte is a component which is contained in the blood, is different from a component occurring only in a red blood cell, and can generate hydrogen peroxide upon the reaction with an oxidase. Whole blood is utilized in the method. The method comprises the steps of hemolyzing the whole blood and detecting hydrogen peroxide generated by the reaction between the analyte and the oxidase. The measurement method can avoid the inhibition of color development by hemoglobin and the interference with the measurement by hemoglobin. Further it can be used for biological tests that are carried out in a household, an individual doctor's clinic or at the bedside of patients without the need for any blood cell separation procedure or the like, because the measurement utilizes whole blood.
    Type: Grant
    Filed: July 22, 2009
    Date of Patent: November 11, 2014
    Assignee: Nippon Kayaku Kabushiki Kaisha
    Inventors: Reiko Machida, Yayoi Irie, Masahiko Yabuuchi, Yoshihiko Umegae
  • Publication number: 20140329258
    Abstract: A test strip to assist in determining the concentration of an analyte in a fluid sample comprises a base, at least one tab and a break line. The base includes a capillary channel and a test element. The capillary channel is in fluid communication with the test element. The test element is adapted to receive the fluid sample. The at least one tab is removably attached to the base. The capillary channel extends from the base into a portion of the tab. The break line intersects the capillary channel in which an inlet to the capillary channel is exposed along the break line when the tab is separated from the base.
    Type: Application
    Filed: July 15, 2014
    Publication date: November 6, 2014
    Inventor: Roger Fleming
  • Patent number: 8852953
    Abstract: The invention relates to novel immunogens, antibodies, methods and kits for use in immunoassays to detect and quantify zaleplon, metabolites of zaleplon and indiplon. These are the first described immunoassays for these compounds and have greater sensitivity than alternative analytical techniques.
    Type: Grant
    Filed: July 16, 2010
    Date of Patent: October 7, 2014
    Assignee: Randox Laboratories Ltd.
    Inventors: Elouard Benchikh, Stephen Peter Fitzgerald, Philip Andrew Lowry, Ivan Robert McConnell
  • Publication number: 20140273055
    Abstract: A noninvasive method for diagnosing skin health in a subject comprising collecting a skin sample/epithelial cell sample from the subject; detecting a level of one or more biomarkers selected from the group consisting of Myeloperoxidase and oxidized lipids in the epithelial cell sample/skin cell sample; diagnosing the subject as having oxidative stress and/or oxidative damage based on the level of a detected biomarker. Further, a noninvasive method for evaluating the efficacy of products for skin health.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Applicant: The Procter & Gamble Company
    Inventors: Kathleen Marie KERR, Angela Marie FIENO, Kenneth Robert WEHMEYER, Lijuan LI, James Robert SCHWARTZ, Raymond Alan GRANT
  • Patent number: 8834878
    Abstract: A method for the treatment of cancer is provided wherein a cancer recognition (CARE) antigen or CARE antibody is administered to a patient. Administration of the CARE antigen or antibody will induce an immune or promote a response of IgM CARE antibodies which will bind to CARE antigen bound to cancer cells, inducing an immune response to destroy the cancer cells. Subsequent to said treatment, ELISA assays may be used to detect levels of said CARE antigen to monitor the efficacy of the treatment, and to govern the further administration of CARE antigens.
    Type: Grant
    Filed: June 6, 2007
    Date of Patent: September 16, 2014
    Inventor: Jerry T. Thornthwaite
  • Patent number: 8822168
    Abstract: The present invention includes assays and kits for detecting the assembly of an RNA binding protein-RNA complex and for detecting the activity of an RNA binding protein.
    Type: Grant
    Filed: March 10, 2006
    Date of Patent: September 2, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Gideon Dreyfuss, Lili Wan, Elizabeth Ottinger
  • Patent number: 8815532
    Abstract: Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays.
    Type: Grant
    Filed: May 23, 2012
    Date of Patent: August 26, 2014
    Assignee: Diagnostic Innovations, LLC
    Inventor: Victor Kovalenko
  • Publication number: 20140234874
    Abstract: The invention provides compositions, kits, and methods for performing colorimetric analysis. A substrate is reacted to generate a chromogenic reaction product, and a reaction stop reagent that is a sulfonic acid is added to stop and stabilize the reaction product. The absorbance properties of the chromogenic reaction product can be maintained over significantly longer periods of time of that of conventional reagents and methods. The sulfonic acid can be used in assays such as ELISAs in order to provide a more accurate and safer detection of analytes in a biological sample.
    Type: Application
    Filed: April 30, 2014
    Publication date: August 21, 2014
    Applicant: SurModics, Inc.
    Inventors: Gary Opperman, Wendy Nelson
  • Publication number: 20140220612
    Abstract: The invention provides novel fluorinated resorufin compounds that are of use in a variety of assay formats. Also provided are methods of using the compounds and kits that include a compound of the invention and instructions detailing the use of the compound in one or more assay formats.
    Type: Application
    Filed: April 8, 2014
    Publication date: August 7, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Robert BATCHELOR, Yue GE, Iain JOHNSON, Wai-Yee LEUNG, Jixiang LIU, Brian PATCH, Peter SMALLEY, Thomas STEINBERG, Kyle GEE
  • Patent number: 8795646
    Abstract: The present application relates generally to oral pharmaceutical formulations for the treatment of human canities.
    Type: Grant
    Filed: September 7, 2012
    Date of Patent: August 5, 2014
    Inventor: Garfield Coore
  • Publication number: 20140212895
    Abstract: The invention provides for detecting target subpopulations of cells that have high proliferative and renewal properties in animals, including circulating tumor cells, cancer stem cells, hematopoietic stem cells and endothelial progenitor cells. The invention utilizes a defined substrate and media of known property to enrich target cell subpopulations which can be used for future genetic, proteomic and morphological analyses. The method can use image-capture and analysis software to characterize cells based on physical properties, such as size, morphology and kinetic properties.
    Type: Application
    Filed: January 24, 2014
    Publication date: July 31, 2014
    Applicant: Xcell Biosciences, Inc.
    Inventor: James Lim
  • Publication number: 20140206013
    Abstract: An imaging method utilizing a split peroxidase is described herein. Imaging methods involve contacting a cell with a split peroxidase and a substrate thereof to allow conversion of a substrate into a product via an enzymatic reaction catalyzed by the reconstitute split peroxidase. Also disclosed herein are split peroxidases, related products and kits.
    Type: Application
    Filed: January 16, 2014
    Publication date: July 24, 2014
    Applicant: Massachusetts Institute of Technology
    Inventors: Alice Y. Ting, Jeffrey Daniel Martell