Mutation Or Genetic Engineering Apparatus Patents (Class 435/285.1)
  • Patent number: 10842611
    Abstract: According to various embodiments, there is provided a tissue scaffold device including a porous core including a plurality of fibres; and an outer portion at least substantially surrounding the porous core, the outer portion including a plurality of pores elongated along a longitudinal axis of the tissue scaffold device.
    Type: Grant
    Filed: November 3, 2015
    Date of Patent: November 24, 2020
    Assignee: NATIONAL UNIVERSITY OF SINGAPORE
    Inventors: Eng San Thian, Zuyong Wang, Ee Jen Wilson Wang, Minghui Hong
  • Patent number: 10801008
    Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.
    Type: Grant
    Filed: July 15, 2020
    Date of Patent: October 13, 2020
    Assignee: Inscripta, Inc.
    Inventors: Phillip Belgrader, Don Masquelier, Andrew Garst, Richard Fox
  • Patent number: 10704991
    Abstract: A cell aspiration support system includes an image acquisition unit, an image processing unit, a display unit, an operation control unit and a cooling unit. The image acquisition unit acquires an image of the group of cells. The image processing unit identifies a cell from the acquired image to calculate feature quantities of each cell, and detects a cell whose feature quantities satisfy predetermined conditions. The display unit displays information relating to the group of cells in a state in which the detected cell is distinguishable. When receiving designation of the specific cell based on the information, the operation control unit moves the cell culture container such that a specific cell is placed at a predetermined aspiration position and moves an aspiration tip to the aspiration position. The cooling unit holds a specimen rack storing an aspirated tip after aspiration and cools the tip.
    Type: Grant
    Filed: August 8, 2018
    Date of Patent: July 7, 2020
    Assignee: Yokogawa Electric Coporation
    Inventors: Takayuki Kei, Hironori Takai
  • Patent number: 10557113
    Abstract: A picktool manipulator device collects a specimen from a culture medium. In a first mode of operation, a picktool is allowed to move in an axial direction relative to support structure of the device. A detector may generate a signal in response to movement of the body in the axial direction so as to determine a height at which the picktool contacts the medium. The device may operate in the first mode when collecting a specimen from a culture medium. A second mode of operation constrains or precludes axial movement of the picktool. In some cases, the device may operate in the second mode when receiving a new picktool or discarding a used picktool.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: February 11, 2020
    Assignee: BD KIESTRA B.V.
    Inventors: Martijn Xander Berntsen, Jurjen Sinnema, Martijn Kleefstra
  • Patent number: 10435661
    Abstract: This invention provides novel tools for surgery on single cells and substrates/devices for delivery of reagents to selected cells. In certain embodiments the substrates comprise a surface comprising one or more orifices, where nanoparticles and/or a thin film is deposited on a surface of said orifice or near said orifice, where the nanoparticles and/or a thin film are formed of materials that heat up when contacted with electromagnetic radiation. In certain embodiments the pores are in fluid communication with microchannels containing one or more reagents to be delivered into the cells.
    Type: Grant
    Filed: October 24, 2014
    Date of Patent: October 8, 2019
    Assignee: The Regents of the University of California
    Inventors: Pei-Yu E. Chiou, Ting-Hsiang S. Wu, Sheraz Kalim Butt, Michael A. Teitell
  • Patent number: 10391233
    Abstract: Disclosed is a drug delivery kit, including an apparatus for preparing a drug delivery system, which includes therein a drug carrier in a gel phase or a solid phase under the airtight condition, and has therein a mixing space configured such that a drug for forming a drug delivery system with the drug carrier is injected from the outside and is mixed with the drug carrier, and a syringe for injecting the drug into the apparatus for preparing a drug delivery system. Also, an apparatus for preparing a drug delivery system, which can be applied to the drug delivery kit, and a method of preparing a drug delivery system using the apparatus are provided. The drug delivery system can be easily prepared on site, whereby the drug can be delivered in vivo.
    Type: Grant
    Filed: April 19, 2016
    Date of Patent: August 27, 2019
    Assignee: TGEL BIO CO., LTD.
    Inventors: Changsoon Hwang, Sun Jong Kim, Han Weon Cho, Sunhee Ham, Hyesook Chung
  • Patent number: 10159952
    Abstract: The invention provides a method for lysing a single cell embedded in a tissue from the inside of the cell, and collecting the intracellular lysate for use in analytical methods. This method preserves the state of molecules of the cell, and therefore allows for transformation of a single target cell in live tissue into a format that can be evaluated using analytical methods.
    Type: Grant
    Filed: June 14, 2017
    Date of Patent: December 25, 2018
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Alexei Finski, Gavin MacBeath
  • Patent number: 10119151
    Abstract: Systems and methods for manipulating molecular material are provided. In one aspect, for example, a method for manipulating molecular material may include positioning an uncharged needle structure in electrical proximity with a charged molecular material at a first locus in a liquid environment, charging the needle structure such that at least a portion of the charged molecular material becomes associated with the needle structure, moving the needle structure and the first locus relative to one another, and discharging the needle structure to disassociate the charged molecular material at a second locus.
    Type: Grant
    Filed: December 11, 2013
    Date of Patent: November 6, 2018
    Assignee: Brigham Young University
    Inventors: Quentin T. Aten, Larry L. Howell, Brian D. Jensen, Sandra Burnett
  • Patent number: 10101250
    Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: October 16, 2018
    Assignee: Berkeley Lights, Inc.
    Inventors: Mark P. White, Randall D. Lowe, Jr.
  • Patent number: 9775917
    Abstract: Described herein are compositions in nanofiber form including one or more bioactive compounds releasably incorporated thereon. In one embodiment a composition is described comprising at least one nanofiber and at least one bioactive compound. The nanofibers are formed from a base material that is solubilized with the bioactive or bioactives in an aqueous based solvent solution and the base material and bioactives are together spun via electrospinning to form dry fibers with the bioactives chemically bonded to the nanofibers and the bioactives remaining stable during storage of the composition under ambient conditions substantially free of moisture. On exposure to moisture, the nanofibers dissolve, thereby releasing the bioactives.
    Type: Grant
    Filed: March 12, 2014
    Date of Patent: October 3, 2017
    Assignee: Active Fibres Limited
    Inventors: Glenn Francis Vile, Iain Cameron Hosie, Simon Vaughan Feasey
  • Patent number: 9766228
    Abstract: The invention relates to a biocompatible scaffold for three dimensional cultivation of cells, said scaffold comprise one or more fibers randomly oriented to form a scaffold with open spaces for cultured cells. The one or more fibers are also coated with a bio-active coating and have a diameter of 100-3000 nm.
    Type: Grant
    Filed: May 16, 2012
    Date of Patent: September 19, 2017
    Assignee: 3Dtro AB
    Inventors: Till Benjamin Puschmann, Milos Pekny, Carl Zanden, Johan Liu
  • Patent number: 9422520
    Abstract: System for generating the motion of a tool, in particular for the work on biological cell material, which includes an apparatus, the apparatus providing a tool section, at which a tool can be arranged, and at least one actuator element arranged to move the tool section, the system further comprising a system control device.
    Type: Grant
    Filed: December 23, 2010
    Date of Patent: August 23, 2016
    Assignee: EPPENDORF AG
    Inventors: Andreas Schirr, Jens Lembke, Dieter Knofe, Andreas Graff
  • Patent number: 9394547
    Abstract: The present invention is concerned with a system and method for introducing a substance into cells. The system has an assembly including a plurality of elongate non-hollow nanoneedles forming a nanoneedle array or patch for delivering the substance into the cells, at least some of the nanoneedles have a non-uniform diameter with a wider upper end, a narrower lower end for penetration into the cells and a length from substantially 200 nm to 100 um. The lower end has a diameter from substantially 20-436 nm. Adjacent nanoneedles are spaced apart by substantially 5-50 um. The nanoneedles are made from a material selected from the group consisting of diamond, cubic boron nitride, carbon nitride, boron nitride, boron carbon nitride, metal borides and essentially boron materials, allowing the nanoneedles to maintain sufficient thinness and yet adequate rigidity during penetration. The nanoneedles are applied onto the cells grown on substrates at a preferred rate from 1 to 5 m/s.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: July 19, 2016
    Assignee: CITY UNIVERSITY OF HONG KONG
    Inventors: Xianfeng Chen, Wenjun Zhang, Peng Shi
  • Patent number: 9352136
    Abstract: The present invention relates to a nano-enhanced device for substance transfer between the device and a tissue. The device comprises a substrate with substantially aligned carbon nanotubes anchored within the substrate, and with at least one end of the carbon nanotubes protruding from the substrate. The protruding nanotube ends may be coated with a drug for delivery of the drug into body tissue. The present invention may be incorporated into an angioplasty catheter balloon or into a patch that is worn on the skin. The carbon nanotubes can be grouped in clusters to effectively form nano-needles which can transfer fluid to or from the subdermal tissue. The nano-needles can be used in conjunction with a sensor to ascertain body fluid information such as pH, glucose level, etc.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: May 31, 2016
    Assignee: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Morteza Gharib, Adrianus I. Aria, Elijah Bodhi Sansom
  • Patent number: 9354156
    Abstract: The method, device, and system relate to particle analysis, and in particular, to a microfluidic device designed for trapping particles for analysis. Particles include beads and cells.
    Type: Grant
    Filed: January 25, 2008
    Date of Patent: May 31, 2016
    Assignee: EMD Millipore Corporation
    Inventors: Philip Janmin Lee, Paul Ju-Sung Hung, Narendra Maheshri
  • Patent number: 9205173
    Abstract: A nerve graft includes a lyophobic substrate, a carbon nanotube film structure, a protein layer, and a nerve network. The carbon nanotube film structure is located on a surface of the lyophobic substrate. The protein layer is located on a surface of the carbon nanotube film structure away from the lyophobic substrate. The nerve network is positioned on a surface of the protein layer away from the lyophobic substrate.
    Type: Grant
    Filed: December 30, 2010
    Date of Patent: December 8, 2015
    Assignees: Tsinghua University, HON HAI PRECISION INDUSTRY CO., LTD.
    Inventors: Li Fan, Chen Feng, Wen-Mei Zhao
  • Patent number: 9163338
    Abstract: The invention relate to fibrous mats comprising chitosan nanofibers and, optionally, at least one filler material, at least one additive, or both. The invention also relates to methods of making same, and devices that include a fibrous mat comprising chitosan nanofibers.
    Type: Grant
    Filed: April 11, 2008
    Date of Patent: October 20, 2015
    Assignee: Drexel University
    Inventors: Caroline L. Schauer, Jessica D. Schiffman
  • Patent number: 9101937
    Abstract: A temperature controlling unit (X1) includes a holder (11) for a liquid receiver (40), a heating block (12) for heating the liquid in the liquid receiver (40), and a cooling block (13) for cooling the liquid in the liquid receiver (40). The holder (11) maintains a first temperature for keeping the temperature of the liquid in the liquid receiver (40) at a lower target temperature. The heating block (12) maintains a second temperature higher than a higher target temperature above the lower target temperature. The cooling block (13) maintains a third temperature lower than the lower target temperature. A temperature controlling method of the present invention includes a heating step for bringing a heating block (12) into contact with the liquid receiver (40) held by the holder (11) and a cooling step for bringing a cooling block (13) into contact with the liquid receiver (40) held by the holder (11).
    Type: Grant
    Filed: October 29, 2010
    Date of Patent: August 11, 2015
    Assignee: ARKRAY, Inc.
    Inventors: Shigeru Kitamura, Naoyuki Nakanishi
  • Publication number: 20150132795
    Abstract: Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
    Type: Application
    Filed: September 8, 2014
    Publication date: May 14, 2015
    Applicant: GeneWeave Biosciences, Inc.
    Inventors: Ryan C. GRISWOLD, Bruce J. RICHARDSON, Rick V. STELLMACHER, Victor H. YEE
  • Patent number: 9017991
    Abstract: Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition.
    Type: Grant
    Filed: September 13, 2011
    Date of Patent: April 28, 2015
    Assignee: Tufts University
    Inventor: Thomas J. Diefenbach
  • Patent number: 8990023
    Abstract: An automated cell injection system and method are described, which can perform automatic, reliable, and high-throughput cell injection of foreign genetic materials, proteins, and other compounds. The system and method overcome the problems inherent in traditional manual injection that is characterized by poor reproducibility, human fatigue, and low throughput. The present invention is particularly suited for zebrafish embryo injection but can be readily extended to other biological injection applications such as mouse embryo, drosophila embryo, and C. elegans injections, capable of facilitating high-throughput genetic research at both academic and industry levels. A novel vacuum based cell-holding device is also provided.
    Type: Grant
    Filed: February 25, 2007
    Date of Patent: March 24, 2015
    Inventors: Yu Sun, Wenhui Wang, Xinyu Liu
  • Patent number: 8980604
    Abstract: The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. The present invention also relates to a mutant glycerol dehydrogenase (GlyDH) comprising at least one amino acid residue in the protein sequence of the parent enzyme replaced by a different amino acid residue at the same position wherein the mutant enzyme has retained more than 50% of the glycerol dehydrogenase activity of the parent enzyme and the glycerol dehydrogenase activity of the mutant GlyDH is less inhibited by NAD+ and/or by its substrate as compared to the parent enzyme and/or by its product as compared to the parent enzyme.
    Type: Grant
    Filed: July 30, 2010
    Date of Patent: March 17, 2015
    Assignee: Metabolic Explorer
    Inventors: Francois Voelker, Laurence Dumon-Seignovert, Isabelle Meynial-Salles, Philippe Soucaille
  • Publication number: 20150037890
    Abstract: In exemplary implementations, transplantation of nucleic acids into cells occurs in microfluidic chambers. The nucleic acids may be large nucleic acid molecules with more than 100 kbp. In some cases, the microfluidic chambers have only one orifice that opens to a flow channel. In some cases, flow through a microfluidic chamber temporarily ceases due to closing one or more valves. Transplantation occurs during a period in which the contents of the chambers are shielded from shear forces. Diffusion, centrifugation, suction from a vacuum channel, or dead-end loading may be used to move cells or buffers into the chambers.
    Type: Application
    Filed: July 31, 2014
    Publication date: February 5, 2015
    Applicant: Massacusetts Institute of Technology
    Inventors: Andreas Mershin, James Pelletier, Neil Gershenfeld, John Glass, Elizabeth Strychalski
  • Patent number: 8945486
    Abstract: A microwell device is provided. The device includes a plate having a upper surface. The upper surface has first and second recesses formed therein. Each recess has an outer periphery. First and second portions of microwells are formed in upper surface of the plate. The first portion of microwells are spaced about the outer periphery of the first recess and the second portion of microwells spaced about the outer periphery of the first recess. A first barrier is about a first portions of the microwells for fluidicly isolating the first portion of the microwells and a second barrier about a second portions of microwells for fluidicly isolating the second portion of the microwells.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: February 3, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Jay W. Warrick, John Yin, Stephen M. Lindsay
  • Publication number: 20150024496
    Abstract: Disclosed herein include embodiments related to delivery of molecular molecules by a sperm cell to an egg cell for expression, including transient expression, in a fertilized progeny. Further embodiments relate to computerized systems for assisting in the disclosed methods.
    Type: Application
    Filed: July 19, 2013
    Publication date: January 22, 2015
    Inventors: Roderick A. Hyde, Tony S. Pan, Lowell L. Wood, JR.
  • Patent number: 8936766
    Abstract: A lipoaspirate collection device to aid in the collection and processing of human tissue and fluid obtained during liposuction for use in point-of-care cell therapy. The collection device includes a collection body and a collection cap. The collection cap may have a fluid port, a lipoaspirate port, a vacuum port, and a relief valve. Within the central cavity of the collection device, a cone shaped may be positioned such that the apex of the cone is positioned underneath the lipoaspirate inlet through which the lipoaspirate fluid and tissue are introduced.
    Type: Grant
    Filed: October 4, 2011
    Date of Patent: January 20, 2015
    Assignee: SpineSmith Partners, L.P.
    Inventors: Ted Sand, Kevin Dunworth, Greg Forman
  • Publication number: 20150004678
    Abstract: The invention relates to an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material with the object of providing an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material whereby a biological material can be efficiently introduced into a host. The invention comprises: one or more packing units in which a mixture solution containing a large number of magnetic supports carrying a biological material to be introduced into a host such as cells upon using, together with a large number of the hosts in a liquid is pooled; and an introduction treatment unit in which a magnetic force affecting the inside of the packing unit is controlled so as to move the magnetic supports relatively with respect to the host so that the biological material can be introduced into the host.
    Type: Application
    Filed: October 9, 2013
    Publication date: January 1, 2015
    Applicants: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventors: Hideji TAJIMA, Yoshiro OKAMI
  • Publication number: 20140329325
    Abstract: A device for use in laser optical transfection of biological targets including an optofluidic microdevice and a piece of optical glass. The optofluidic microdevice has a central vertical outlet and a microchannel network that includes a plurality of entrapping channels with narrowings. The microchannel network is fused with the optical glass. In one aspect the device is used with a petri dish having an optical window. In another aspect the device is used with a well plate having a plurality of wells and associated optical windows. In a third aspect the device is used with a barrier. Each of the aspects forms a peripheral space around the optofluidic microdevice capable of retaining a live culture of biological targets and material that is desired to be injected into those biological targets. Polymer tubing is inserted into the central vertical outlet which connects the device to an external pump.
    Type: Application
    Filed: April 18, 2014
    Publication date: November 6, 2014
    Inventor: David Fozdar
  • Publication number: 20140315282
    Abstract: A microinjector needle mounting module and a needle holder assembly, comprising a micro-injection needle (1), a needle seat (2) and a needle holder (3); the microinjector mounting module is formed by means of inserting the rear end of the microinjector (1) into a hole in the base (21) of the needle seat; the needle holder (3) is provided with an inner hole (32) passing through the barrel body; a conical socket (24) is provided inside the needle seat (2) and one end of the needle-holder (3) is provided with a conical connector (31), said conical connector (31) and conical socket (24) thus constituting a mutually-complementary insertion connection. In addition to a micro-injection needle being assembled with the needle seat to form a mounting module, other micro-operation needles such as holding pipettes or biopsy needles can also be assembled into a mounting module with said needle seat.
    Type: Application
    Filed: January 10, 2013
    Publication date: October 23, 2014
    Inventor: Jianjun Peng
  • Publication number: 20140315311
    Abstract: The invention relates to a cell modification device, comprising a centrifugation chamber with at least one cell modifying surface with a normal vector having an angle of 135-45° to the rotational axis of the centrifugation chamber, wherein the centrifugation chamber comprises at least one input/output port and the cells to be modified are immobilized at the cell modifying surfaces by the rotation of the centrifugation chamber at 2 to 2000 g.
    Type: Application
    Filed: November 13, 2012
    Publication date: October 23, 2014
    Applicant: MILTENYI BIOTEC GMBH
    Inventors: Stefan Miltenyi, Alexander Scheffold
  • Patent number: 8852508
    Abstract: The present invention discloses a microinjection apparatus (100) for microinjection of substances into individual substances comprising at least one carrier (120, 130) on which at least one sample is immobilizable. In embodiments, the apparatus comprises at drivable support (110) on which at least one carrier is positioned, wherein the support drives the at least one carrier in a closed loop to a respective plurality of stations along the loop. The plurality of stations constitutes at least one sample-substance-providing station (141), at least one sample-substance microinjection station (142) and at least one sample-extraction station (143).
    Type: Grant
    Filed: January 22, 2009
    Date of Patent: October 7, 2014
    Assignee: CSEM Centre Suisse d'Electronique et de Microtechnique SA—Recherche et Developpement
    Inventors: Siegfried Graf, Helmut Knapp
  • Publication number: 20140295558
    Abstract: The present invention is concerned with a system and method for introducing a substance into cells. The system has an assembly including a plurality of elongate non-hollow nanoneedles forming a nanoneedle array or patch for delivering the substance into the cells, at least some of the nanoneedles have a non-uniform diameter with a wider upper end, a narrower lower end for penetration into the cells and a length from substantially 200 nm to 100 um. The lower end has a diameter from substantially 20-436 nm. Adjacent nanoneedles are spaced apart by substantially 5-50 um. The nanoneedles are made from a material selected from the group consisting of diamond, cubic boron nitride, carbon nitride, boron nitride, boron carbon nitride, metal borides and essentially boron materials, allowing the nanoneedles to maintain sufficient thinness and yet adequate rigidity during penetration. The nanoneedles are applied onto the cells grown on substrates at a preferred rate from 1 to 5 m/s.
    Type: Application
    Filed: January 23, 2014
    Publication date: October 2, 2014
    Applicant: CITY UNIVERSITY OF HONG KONG
    Inventors: Xianfeng CHEN, Wenjun ZHANG
  • Publication number: 20140273180
    Abstract: Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity.
    Type: Application
    Filed: October 8, 2013
    Publication date: September 18, 2014
    Applicant: GENEWEAVE BIOSCIENCES, INC.
    Inventors: Ryan C. GRISWOLD, Kenneth G. OLSON, Bruce J. RICHARDSON, Rick V. STELLMACHER, Victor H. YEE
  • Patent number: 8778663
    Abstract: A thermalcycler includes a first thermalcycler body section having a first face and a second thermalcycler body section having a second face. A cavity is formed by the first face and the second face. A thermalcycling unit is positioned in the cavity. A heater trace unit is connected to a support section, to the first thermalcycler body section, to the second thermalcycler body section, and to the thermalcycling unit. The first thermalcycler body section and the second thermalcycler body section are positioned together against the support section to enclose the thermalcycling unit and the heater trace unit.
    Type: Grant
    Filed: September 18, 2007
    Date of Patent: July 15, 2014
    Assignee: Lawrence Livermore National Security, LLC.
    Inventors: William J. Benett, John T. Andreski, John M. Dzenitis, Anthony J. Makarewicz, Dean R. Hadley, Satinderpall S. Pannu
  • Patent number: 8765077
    Abstract: Multiplex binding assay assemblies are disclosed. The assemblies generally include at least one assay bar that includes a top side, a bottom side, and at least one well accessible from the top side of the assay bar. Each well includes a side surface, a bottom surface, an open top end, and at least one secondary container, with each secondary container including a capillary tube that (i) begins at a location within an interior volume of the well and (ii) ends at a location beneath the bottom surface of the assay bar. The assemblies further include a dispenser bar that is adapted to be positioned adjacent to the top side of the assay bar, which includes one or more reservoirs that are configured to provide one or more reagents to the at least one secondary container located in each well of the assay bar.
    Type: Grant
    Filed: August 2, 2011
    Date of Patent: July 1, 2014
    Inventor: Pierre F. Indermuhle
  • Patent number: 8765078
    Abstract: There is provided a biochip stamping device. The biochip stamping device includes a stamping jig in which a first biochip is aligned; an inverting mechanism vertically inverting a second biochip; and a movement mechanism transferring the vertically inverted second biochip on the stamping jig to combine the first biochip and the second biochip.
    Type: Grant
    Filed: January 26, 2012
    Date of Patent: July 1, 2014
    Assignee: Samsung Electro-Mechanics Co., Ltd.
    Inventors: Sang Youl Jeon, Bo Sung Ku, Hee Ju Son
  • Patent number: 8697005
    Abstract: Multiplex binding assay assemblies are disclosed. The assemblies include at least one assay bar that has a top side, a bottom side, and at least one well accessible from the top side of the assay bar, with each well including a side surface, a bottom surface, an open top end. Each well also includes at least one secondary container, with each secondary container including a capillary tube that (i) begins at a location within an interior volume of the well and (ii) ends at a location beneath the bottom side of the assay bar. The assemblies further include a guiding track, which includes a set of two rails, with each rail having its own separate groove. Such grooves are configured to run parallel to each other with a distance between such grooves, with a first groove configured to receive a protruding element (or an end) of a first side of the assay bar, and a second groove configured to receive a protruding element (or an end) of a second side of the assay bar.
    Type: Grant
    Filed: August 2, 2011
    Date of Patent: April 15, 2014
    Inventor: Pierre F. Indermuhle
  • Publication number: 20140093946
    Abstract: An embodiment of a system is provided herein, wherein the system allows for the analysis and selection of numerous experimental conditions to optimize transfection efficiency and cell viability. The system is used for magnetic particle based nucleic acid delivery by optimizing various parameters. The system comprises a control module; an incubation module for incubating magnetic nanoparticle and nucleic acid; a transfection module and an analysis module.
    Type: Application
    Filed: September 27, 2013
    Publication date: April 3, 2014
    Applicant: General Electric Company
    Inventors: Jean-Baptiste Mathieu, Brian Michael Davis, Vasile Bogdan Neculaes, Evelina Roxana Loghin, Kenneth Roger Conway
  • Patent number: 8623296
    Abstract: Microfluidic devices, assemblies, and systems are provided, as are methods of manipulating micro-sized samples of fluids. Microfluidic devices having a plurality of specialized processing features are also provided.
    Type: Grant
    Filed: August 4, 2010
    Date of Patent: January 7, 2014
    Assignee: Applied Biosystems, LLC
    Inventors: Sean M. Desmond, Zbigniew T. Bryning, John Shigeura, Gary Lim, Adrian Fawcett, Jacob K. Freudenthal, Gary Bordenkircher
  • Publication number: 20130345094
    Abstract: The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells. The invention system is useful for isolating somatic cells from tissue samples, producing iPSC lines from adult differentiated cells by reprogramming such cells, identifying the pluripotent reprogrammed adult cells among other cells, and expanding and screening the identified reprogrammed cells.
    Type: Application
    Filed: November 30, 2012
    Publication date: December 26, 2013
    Applicant: New York Stem Cell Foundation
    Inventor: New York Stem Cell Foundation
  • Patent number: 8614088
    Abstract: The present invention relates to a system and method for the infiltration of plants in a continuous or quasi-continuous operation mode. The system and method of the present invention combine mechanical (automatic and manual) movable and manipulable units and/or containers with vacuum infiltration chambers to enable large-scale infiltration of plants.
    Type: Grant
    Filed: January 23, 2009
    Date of Patent: December 24, 2013
    Assignee: Bayer Innovation GmbH
    Inventors: Ralf Dujardin, Sebastian Schmidt, Markus Zamponi
  • Patent number: 8580544
    Abstract: The invention relates to an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material with the object of providing an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material whereby a biological material can be efficiently introduced into a host. The invention comprises: one or more packing units in which a mixture solution containing a large number of magnetic supports carrying a biological material to be introduced into a host such as cells upon using, together with a large number of the hosts in a liquid is pooled; and an introduction treatment unit in which a magnetic force affecting the inside of the packing unit is controlled so as to move the magnetic supports relatively with respect to the host so that the biological material can be introduced into the host.
    Type: Grant
    Filed: October 16, 2003
    Date of Patent: November 12, 2013
    Assignees: Universal Bio Research Co. Ltd.
    Inventors: Hideji Tajima, Yoshiro Okami
  • Patent number: 8569041
    Abstract: The present invention relates to automated methods of introducing multiple nucleic acid sequences into one or more target cells.
    Type: Grant
    Filed: March 5, 2012
    Date of Patent: October 29, 2013
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Harris H. Wang, Farren J. Isaacs
  • Patent number: 8545759
    Abstract: A pair or receptacles capable of housing an emitter probe and a detector probe are installed inside a bioreactor to monitor the properties of the nutrient media without contacting the nutrient media.
    Type: Grant
    Filed: October 21, 2011
    Date of Patent: October 1, 2013
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz Niazi
  • Publication number: 20130210123
    Abstract: A biomaterial handling device is described that will provide for functionality for performing both IVF procedures and washing techniques in a single device. Disclosed embodiments provide increased protection to biomaterial samples during processing and handling. Embodiments of the invention reduce labor intensive processes for both IVF and washing treatments and address reduced risks of contamination of biological samples by providing an increasingly sterile environment.
    Type: Application
    Filed: February 13, 2013
    Publication date: August 15, 2013
    Inventors: JOAN MALCOLM, Shannon Byers, Rob Taft, Paul C. Sabin, Andrew David Zdeblick, Ivan Goryachev
  • Patent number: 8507258
    Abstract: A method of perforating a membrane and an apparatus therefor is presented. The method includes bringing a membrane-denaturing substance into contact with or close proximity to at least a site of the membrane, the substance inducing a membrane-denaturing reaction by a stimulus; providing the stimulus to the substance so as to denature the membrane; and perforating the membrane with a membrane-destroying member. The stimulus is carried through the membrane-destroying member. The present invention eliminates the influence of the membrane-denaturing agent on the substance to be injected into the cell. The present invention also enables applying the stimulus locally, with a simple construction.
    Type: Grant
    Filed: November 27, 2007
    Date of Patent: August 13, 2013
    Assignee: Akita Prefectural University
    Inventor: Takashi Saito
  • Publication number: 20130164828
    Abstract: An integrated fibre based device for transfecting material into a cell comprising an optical fiber that has a lens formed at its end for directing light to a surface of the cell, and a channel for delivery of the material for transfection into the cell.
    Type: Application
    Filed: June 10, 2011
    Publication date: June 27, 2013
    Applicant: University Court of The University of St. Andrews
    Inventors: Kishan Dholakia, Na Ma, Praveen Cheriyan Ashok, David Stevenson, Francis James Gunn-Moore
  • Publication number: 20130102060
    Abstract: Systems, devices, and methods for delivering a biological material into a biological structure are provided. In one aspect, for example, a lance device for introducing biological material into a biological structure and configured for use in a nanoinjection system including a microscope is provided. Such a device can include a substrate including a handle region located between a manipulator coupling region and a lance shaft region, a lance tip operable to introduce biological material into a biological structure, the lance tip being coupled to the lance shaft region, and an electrically conductive layer extending from the manipulator coupling region to the lance tip, the conductive layer being configured to electrically couple to a power source. Thus, the conductive layer provides an electrical connection from the power source to the lance tip when in use.
    Type: Application
    Filed: October 22, 2012
    Publication date: April 25, 2013
    Applicant: NANOINJECTION TECHNOLOGIES, L.L.C.
    Inventor: NanoInjection Technologies, L.L.C.
  • Publication number: 20130102078
    Abstract: Systems, devices, and methods for injecting biological material into a micro-object such as a cell are provided. In one aspect, for example, a cellular injection device can include a housing, an injection lance coupled to the housing and having a working tip extending outward from the housing, and a biological material delivery device coupled to the housing and having an effluent tip extending outward from the housing. The effluent tip can be positioned sufficiently proximal to the working tip such that biological material expelled from the effluent tip substantially contacts the working tip. In one aspect, the injection lance is removably coupled to the housing. In another aspect, the biological material delivery device is removably coupled to the housing.
    Type: Application
    Filed: October 22, 2012
    Publication date: April 25, 2013
    Applicant: NANOINJECTION TECHNOLOGIES, L.L.C.
    Inventor: NanoInjection Technologies, L.L.C.
  • Patent number: 8409847
    Abstract: Systems and methods are provided for controlling the diameter of a mammalian hybrid coronary bypass graft. The system includes a controller having at least one input for receiving information and feedback information and an output for outputting control signals, including at least one steady flow system control signal; and a pressure/flow loop subsystem coupled to the controller. The pressure/flow loop subsystem includes a specimen holder, an external flow loop system coupled to the specimen holder, a steady flow system, and an output for outputting the feedback information. The pressure/flow loop subsystem receives the control signals and is capable of adjusting a diameter of a specimen in accordance with the control signals, when the specimen holder contains the specimen.
    Type: Grant
    Filed: December 28, 2007
    Date of Patent: April 2, 2013
    Assignee: ICE Development Technologies, LLC
    Inventor: Michael Dancu