With Means For Applying An Electric Current Or Charge (e.g., Electrofusion, Electroporation, Etc.) Patents (Class 435/285.2)
  • Patent number: 10363093
    Abstract: A method of inducing local cell death in patient tissue is provided. The method includes generating first and second radiation, conveying the radiation to a focusing element, and focusing the radiation on a target with the focusing element. A system for inducing local cell death in patient tissue is also provided. The system includes a power source for generating narrowband and/or ultra-wideband radiation, and a focusing element for focusing the radiation on a target.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: July 30, 2019
    Assignee: Old Dominion University Research Foundation
    Inventors: Karl H. Schoenbach, Shu Xiao
  • Patent number: 10261089
    Abstract: Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: April 16, 2019
    Assignee: Trustees of Tufts College
    Inventors: David R. Walt, David M. Rissin
  • Patent number: 9982251
    Abstract: An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm.sup.?1) defined by the quotient of the electrode gap squared (cm.sup.2) divided by the chamber volume (cm.sup.3), wherein the geometric factor is less than or equal to 0.1 cm.sup.?1, wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 50 microSiemens/cm to 500 microSiemens/cm, wherein the suspension is enclosed in the chamber during treatment, and b. treating the suspension enclosed in the chamber with one or more pulsed electric fields.
    Type: Grant
    Filed: March 15, 2004
    Date of Patent: May 29, 2018
    Assignee: CELLECTIS S.A.
    Inventors: Richard E. Walters, Alan D. King
  • Patent number: 9526823
    Abstract: A cell treatment device which allows cell dispersion liquid including cancer cells to pass therethrough to cause at least the cancer cells to be subjected to at least one of a physical action, a chemical action, and a biologically activating action, includes: a slit member having a first surface and a second surface and including a through hole having a slit shape penetrating from the first surface to the second surface and extending in one direction, the through hole having a tapered portion, wherein the width of a cross section of the tapered portion orthogonal in the one direction reduces from the first surface toward the second surface side, and is smaller than an average diameter of the cancer cells at an end on the second surfaces side, and the cell dispersion liquid is allowed to pass from the first surface side through the through hole to the second surface side.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: December 27, 2016
    Assignee: Seiko Epson Corporation
    Inventor: Satomi Yoshioka
  • Patent number: 9399771
    Abstract: Described herein are methods and compositions for high efficiency transfection of siRNA into a cell population. Such methods and compositions utilize a low voltage pre-conditioning pulse to modulate the efficiency of siRNA transfection. In some embodiments, the methods and compositions permit spatial and temporal control of siRNA transfection efficiency within a population of cells. The disclosed methods and compositions, in some embodiments, are amenable to high throughput applications such as siRNA library-based phenotypic screening.
    Type: Grant
    Filed: September 25, 2013
    Date of Patent: July 26, 2016
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Arati Sridharan, Jitendran Muthuswamy
  • Patent number: 9382633
    Abstract: Devices, systems and methods for processing cellulosic material to produce fermentable sugars are provided. Devices, systems and methods for increasing fermentation rates of microbes via biostimulation are provided. Electrodes are preferably positioned along an interior or exterior of a tube-shaped component to administer electromagnetic/electric pulses to a solution comprising a microbe. Systems can advantageously be used in new biofuels production plants, or in existing biofuels production plants without the need for significant retrofits.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: July 5, 2016
    Assignee: Colorado Energy Research Technologies, LLC
    Inventors: Wayne J. Powell, Robert D. Boehmer, Lee L. Johnson
  • Patent number: 9382510
    Abstract: An apparatus for electroporation of biological cells is provided. The apparatus includes a sample container having an insulator chamber for holding the cells. The sample container has a first electrode and a second electrode to provide electrical connection for electroporation. The insulator chamber is configured to contain at least one cell monolayer. The apparatus also includes a pulse generator that can generate a predetermined pulse for electroporation of the cells.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: July 5, 2016
    Inventor: Jian Chen
  • Patent number: 9340318
    Abstract: It is intended to provide an object-holding sheet and an object-treating equipment, which are useful for arranging objects such as biological materials (e.g., cells, embryos or individuals) or particles having the similar size thereto and efficiently conducting procedures such as analysis and fractionation on the objects. The present invention provides an object-holding sheet for holding two or more objects, the object-holding sheet including: a sheet member having two or more through-holes; and an anti-drop member corresponding to each of the holes, wherein the holes have a size that enables only one of the objects to be held, and the anti-drop member is disposed in the vicinity of one opening of each of the holes so as not to permit the object held in each of the holes to pass through the opening.
    Type: Grant
    Filed: May 26, 2010
    Date of Patent: May 17, 2016
    Assignee: Canon Kabushiki Kaisha
    Inventors: Tsuyoshi Nomoto, Kohei Watanabe, Takeshi Miyazaki, Toshio Tanaka, Yasuhito Shimada
  • Patent number: 9279101
    Abstract: Devices, systems and methods for processing cellulosic material to produce fermentable sugars are provided. Devices, systems and methods for increasing fermentation rates of microbes via biostimulation are provided. Electrodes are preferably positioned along an interior or exterior of a tube-shaped component to administer electromagnetic/electric pulses to a solution comprising a microbe. Systems can advantageously be used in new biofuels production plants, or in existing biofuels production plants without the need for significant retrofits.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: March 8, 2016
    Assignee: Colorado Energy Research Technologies, LLC
    Inventors: Wayne J. Powell, Robert D. Boehmer, Lee L. Johnson
  • Patent number: 9133496
    Abstract: An object of the present invention is to provide a screening system that targets ion channels and has superior efficiency. The present invention provides a material for screening for compounds that act on a target ion channel, comprising cells which retain at least one first DNA encoding a voltage-dependent Na ion channel that has been inhibited from being inactivated, and in which a K ion channel has been activated so that a resting membrane potential becomes deeper in a negative direction.
    Type: Grant
    Filed: June 29, 2011
    Date of Patent: September 15, 2015
    Assignees: PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY, CHANNELOSEARCH TECHNOLOGY
    Inventors: Yuji Imaizumi, Masato Fujii, Susumu Ohya, Hisao Yamamura
  • Publication number: 20150147803
    Abstract: A glass pipette such as an electrode for electrophysiological recording is coated with quantum dots. This greatly aids the ability to observe the glass pipette, particular in tissue as the quantum dots provide an excellent performance under two-photon illumination used to visualize objects at depths of hundreds of microns.
    Type: Application
    Filed: February 5, 2014
    Publication date: May 28, 2015
    Applicants: Institute of Experimental Medicine of the Hungarian Academy of Sciences, Howard Hughes Medical Institute, The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Igor L. Medintz, Bertalan K. Andrasfalvy, Kimihiro Susumu, James B. Delehanty, Alan L. Huston, John J. Macklin, Mladen Barbic
  • Patent number: 9014800
    Abstract: The present invention provides a system and method of directing, focusing, or concentrating electrical charges within a defined electric field so that these charges can be used to exert forces on cells and tissues in vivo and/or cell cultures in vitro. The present invention reduces and/or eliminates the damage at a target site that would normally be caused by an electrode that acts as a current source or sink to accomplish the same task.
    Type: Grant
    Filed: October 7, 2008
    Date of Patent: April 21, 2015
    Assignee: University of South Florida
    Inventors: Jose I. Rey, Richard Gilbert, Mark J. Jaroszeski, Richard Heller
  • Publication number: 20150093813
    Abstract: An electroporation apparatus comprising an elongated hollow member in order to provide a uniform electric field during electroporation, wherein specifically, electroporation is carried out by applying electric pulses through a couple of electrodes from both end parts of the elongated hollow member, after the hollow member is charged with fluid specimen including cells and material which would be injected into the cells.
    Type: Application
    Filed: November 14, 2014
    Publication date: April 2, 2015
    Inventors: Jun-Keun Chang, Keun-Chang CHO, Chan-Il CHUNG, Young-Shik SHIN, Jeong-Ah KIM, Neon-Cheol JUNG
  • Patent number: 8992835
    Abstract: Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: March 31, 2015
    Assignee: Becton, Dickinson and Company
    Inventors: Gerrit Jan van den Engh, Timothy Wayne Petersen
  • Patent number: 8962305
    Abstract: A biosensor chip with nano-structures. The biosensor chip includes a RF biosensor, comprising an isolated substrate; a ground plane; a filtering circuit; at least one cell detection area with nano-structures and a protection layer. The RF biosensor can detect the existence of the cancer cells, high frequency biological effects and the cells relationship between transfers by noninvasive method. The RF biosensor according to the invention can provide high accuracy and sensitivity in cancer cells detection.
    Type: Grant
    Filed: March 19, 2012
    Date of Patent: February 24, 2015
    Assignee: Kun Shan University
    Inventors: Hung-Wei Wu, Yong-Han Hong, Yu-Fu Chen, Yung-Wei Chen, Shu-Ting Teng
  • Patent number: 8932850
    Abstract: An electroporation apparatus comprising an elongated hollow member in order to provide a uniform electric field during electroporation, wherein specifically, electroporation is carried out by applying electric pulses through a couple of electrodes from both end parts of the elongated hollow member, after the hollow member is charged with fluid specimen including cells and material which would be injected into the cells.
    Type: Grant
    Filed: June 13, 2005
    Date of Patent: January 13, 2015
    Assignee: Invitrogen Singapore Pte. Ltd.
    Inventors: Jun-Keun Chang, Keun-Chang Cho, Chan-Il Chung, Young-Shik Shin, Jeong-Ah Kim, Neon-Cheol Jung
  • Patent number: 8921092
    Abstract: A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode is connected to the upper body. Both electrodes are connected to a pulse generator. Each electrode has a sample contact surface such that the first electrode and the second electrode may be positioned to hold a surface tension constrained sample between the two sample contact surfaces and the sample may receive a selected electric pulse.
    Type: Grant
    Filed: February 12, 2013
    Date of Patent: December 30, 2014
    Assignee: Applied Biosystems LLC
    Inventors: Richard Jarvis, Mike Byrom, Dmitriy Ovcharenko
  • Patent number: 8921041
    Abstract: The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.
    Type: Grant
    Filed: May 17, 2012
    Date of Patent: December 30, 2014
    Assignee: ACEA Biosciences, Inc.
    Inventors: Xiaobo Wang, Yama A. Abassi, Josephine Atienza, Xiao Xu, Junquan Xu
  • Publication number: 20140363881
    Abstract: A pulse generation system for applying electric pulses across a load includes a first plurality of energy storage modules connected in series on a positive chain and configured to apply a positive potential to the load and a second plurality of energy storage modules connected in series on a negative chain and configured to apply a negative potential to the load. Each energy storage module of the positive chain and the negative chain includes a rectifier and a storage element, and at least one control element.
    Type: Application
    Filed: June 6, 2013
    Publication date: December 11, 2014
    Inventors: Antonio Caiafa, Vasile Bogdan Neculaes, Andrew Soliz Torres, Allen Lawrence Garner
  • Patent number: 8906696
    Abstract: A testing device uses a selectively deformable substrate to capture and retain spherical beads for genetic experimentation. A method of fabricating the device is described in which a silicon substrate can be coated with a photosensitive, bio-compatible polymer for photolithographic patterning using a single mask exposure. The polymer is patterned with a matrix of wells, each well capable of expansion to accept placement of a bead in the well, and contraction to secure the bead in the well. The polymer can exhibit piezoelectric properties that cause it to respond mechanically to a selected electrical excitation.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: December 9, 2014
    Assignee: STMicroelectronics Asia Pacific Pte Ltd
    Inventor: Archit Giridhar
  • Patent number: 8835362
    Abstract: A mechanism is provided for utilizing a nanodevice to distinguish molecules with different structure. The molecules translocate through or across a nanochannel filled with a electrolyte solution. An electrical signal through the nanochannel is measured for every translocation event. Inner surfaces of the nanochannel include a functional layer, which is a coating to functionalize the nanochannel, in which the functional layer is configured to interact with predetermined ones of the molecules during translocation events. It is determined that a combination of at least two different molecules is formed based on predetermined ones of the molecules interacting with the functional layer to change the electrical signal and/or change a translocation time for the translocation event.
    Type: Grant
    Filed: December 26, 2012
    Date of Patent: September 16, 2014
    Assignees: International Business Machines Corporation, The University of Melbourne, Florey Institute of Neuroscience and Mental Health
    Inventors: Matthew Downtown, Natalie Gunn, Stefan Harrer, Priscilla Rogers, John Wagner, Ross Bathgate, Daniel James Scott, Stan Skafidas
  • Patent number: 8815574
    Abstract: A system and method is provided for non-invasively measuring changes in a specimen suspected of containing one or more microbes, by monitoring changes in the dielectric constant of the specimen caused by metabolic processes of such microbes.
    Type: Grant
    Filed: December 22, 2005
    Date of Patent: August 26, 2014
    Assignee: Becton, Dickinson and Company
    Inventors: Nicholas R. Bachur, Jr., John W. Neubert
  • Publication number: 20140227785
    Abstract: Systems and methods for manipulating molecular material are provided. In one aspect, for example, a method for manipulating molecular material may include positioning an uncharged needle structure in electrical proximity with a charged molecular material at a first locus in a liquid environment, charging the needle structure such that at least a portion of the charged molecular material becomes associated with the needle structure, moving the needle structure and the first locus relative to one another, and discharging the needle structure to disassociate the charged molecular material at a second locus.
    Type: Application
    Filed: December 11, 2013
    Publication date: August 14, 2014
    Applicant: Brigham Young University
    Inventors: Quentin T. Aten, Larry L. Howell, Brian D. Jensen, Sandra Burnett
  • Patent number: 8785177
    Abstract: Methods for creating a transient nanoscale opening in a cell membrane and methods for transporting a desired species through the nanoscale opening are provided. A nano-sized needle-like tip can be used to mechanically slice the cell membrane to create a transient, localized nanoscale slit. The nanoscale slit may be used for transferring exogenous molecules into a living cell.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: July 22, 2014
    Assignee: The Board of Trustees of the University of Illinois, a body Corporate and Politic of the State of Illinois
    Inventors: Min-Feng Yu, Kyungsuk Yum, Ning Wang
  • Patent number: 8785144
    Abstract: Disclosed is an analysis method comprising the steps of: (a) reacting a substance to be analyzed with at least a specific partner which exhibits a selective interaction with the substance, converting a soluble substance to an insoluble substance by an insolubilization reaction, in correlation with the amount of the substance to be analyzed contained in a sample, and depositing the insoluble substance on a sensing part, and (b) electrically analyzing the insoluble substance deposited on the sensing part, wherein at least one of steps (a) and (b) is carried out under flow conditions.
    Type: Grant
    Filed: March 17, 2009
    Date of Patent: July 22, 2014
    Assignee: Mitsubishi Chemical Medience Corporation
    Inventors: Yasuo Ifuku, Nagamoto Murai
  • Publication number: 20140199765
    Abstract: Systems, devices, and methods for delivering a biological material into a cell are provided. In one example, a lance device for introducing biological material into a cell and configured for use in a nanoinjection system including a microscope is provided. Such a device can include a lance having a tip region and a shaft region, wherein the lance is structurally configured to allow entry and movement of the tip region into the cell along an elongate axis of the tip region and along a focal plane of the microscope. In another example, the lance can be configured to allow substantially horizontal entry and movement of the tip region into the cell.
    Type: Application
    Filed: December 17, 2013
    Publication date: July 17, 2014
    Applicant: Brigham Young University
    Inventors: Quentin T. Aten, Sandra H. Burnett, Brian D. Jensen, Larry L. Howell
  • Patent number: 8765452
    Abstract: Devices, systems and methods for increasing fermentation rates of microbes via biostimulation are provided. Electrodes are preferably positioned along an interior or exterior of a tube-shaped component to administer electromagnetic/electric pulses to a solution comprising a microbe. Systems can advantageously be used in new biofuels production plants, or in existing biofuels production plants without the need for significant retrofits.
    Type: Grant
    Filed: December 26, 2012
    Date of Patent: July 1, 2014
    Assignee: Colorado Energy Research Technologies, LLC
    Inventors: Wayne J. Powell, Robert D. Boehmer, Lee L. Johnson, Ken Swartz, Donald E. Lenci
  • Patent number: 8753573
    Abstract: Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.
    Type: Grant
    Filed: April 26, 2012
    Date of Patent: June 17, 2014
    Assignee: Becton, Dickinson and Company
    Inventors: Gerrit Jan van den Engh, Timothy Wayne Petersen
  • Publication number: 20140127813
    Abstract: Systems, devices, and methods for delivering a biological material into an organelle of a cell are provided. In one aspect, for example, a method for introducing biological material into an organelle of a cell can include bringing into proximity outside of a cell a lance and a preselected biological material, charging the lance with a polarity and a charge sufficient to electrically associate the preselected biological material with a tip portion of the lance, and penetrating an outer portion of the cell with the lance and directing and inserting the lance into the cell but outside of the organelle. The method can further include discharging the lance to release at least a portion of the biological material, charging the lance with an opposite polarity and charge sufficient to electrophoretically drive at least a portion of the biological material into the organelle, and withdrawing the lance from the cell.
    Type: Application
    Filed: October 18, 2013
    Publication date: May 8, 2014
    Applicant: Brigham Young University
    Inventors: Quentin T. Aten, Regis A. David, Sandra H. Burnett, Brian D. Jensen, Larry L. Howell
  • Patent number: 8697446
    Abstract: The present invention relates to a cell fusion chamber in which two types of cells having different diameters are fused, the cell fusion chamber including: a cell fusion region in which cell fusion is carried out; a pair of electrodes formed by a conductor and disposed opposite to each other in the cell fusion region; and a partition wall having at least one fine pore; near the fine pore, a cell fusion device including a cell fusion container containing a cell fusion region; a pair of electrodes; a spacer; and an insulator disposed between the spacer and one of the electrodes and having at least one fine pore; and an electronic power supply which applies an alternating voltage and a voltage pulsed direct current to the electrodes, and a cell fusion method using the same.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: April 15, 2014
    Assignee: Tosoh Corporation
    Inventors: Toru Futami, Takahiro Maruyama, Atsushi Morimoto
  • Publication number: 20140093964
    Abstract: Systems, devices, and methods for delivering a biological material into an organelle of a cell are provided. In one aspect, for example, a method for introducing biological material into an organelle of a cell includes bringing into proximity a lance and a preselected biological material outside of a cell and charging the lance with a polarity and a charge sufficient to electrically associate the preselected biological material with a tip portion of the lance. The method also includes penetrating an outer portion of the cell with the lance and directing and inserting the lance into an organelle, discharging the lance to release at least a portion of the biological material into the organelle, and withdrawing the lance from the cell.
    Type: Application
    Filed: September 30, 2013
    Publication date: April 3, 2014
    Applicant: Brigham Young University
    Inventors: Quentin T. Aten, Sandra H. Burnett, Brian D. Jensen, Larry L. Howell
  • Patent number: 8679827
    Abstract: The present invention relates to methods for measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit. A solution comprising a substrate for said enzyme and at least one air segment contacts the sensor to remove unbound analyte and labeled antibody from a region of the sensor. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.
    Type: Grant
    Filed: June 13, 2012
    Date of Patent: March 25, 2014
    Assignee: Abbott Point of Care Inc.
    Inventors: Graham Davis, Imants Lauks, Chao Lin, Cary James Miller
  • Patent number: 8679806
    Abstract: A real-time method employing a portable peptide-containing potentiometric biosensor, can directly detect and/or quantify bacterial spores. Two peptides for specific recognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolayer immobilization (PMI) technique. The sensors translate the biological recognition event into a potential change by detecting, for example, B. subtilis spores in a concentration range of 0.08-7.3×104 CFU/ml. The sensing method exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectivity coefficients of the sensors for other kinds of spores are in the range of 0-1.0×10?5. The biosensor method not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensing method can distinguish a Bacillus subtilis 1A700 from other B.
    Type: Grant
    Filed: July 21, 2008
    Date of Patent: March 25, 2014
    Assignee: Polytechnic Institute of New York University
    Inventors: Kalle Levon, Bin Yu, Yanxiu Zhou
  • Publication number: 20140080176
    Abstract: The invention provides an organism for expressing foreign DNA, the organism engineered to accept standard DNA carriers. The genome of the organism codes for intracytoplasmic membranes and features an interruption in at least one of the genes coding for restriction enzymes. Further provided is a system for producing biological materials comprising: selecting a vehicle to carry DNA which codes for the biological materials; determining sites on the vehicle's DNA sequence susceptible to restriction enzyme cleavage; choosing an organism to accept the vehicle based on that organism not acting upon at least one of said vehicle's sites; engineering said vehicle to contain said DNA; thereby creating a synthetic vector; and causing the synthetic vector to enter the organism so as cause expression of said DNA.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 20, 2014
    Applicant: UCHICAGO ARGONNE, LLC
    Inventors: Philip D. Laible, Deborah K. Hanson
  • Patent number: 8673623
    Abstract: An apparatus for performing magnetic electroporation is disclosed. A required electric field for electroporation is generated using a pulsed magnetic field through a closed magnetic yoke, such as a toroid, placed in a flow path of a fluid medium to be processed. The fluid medium flows through the orifice of the magnetic yoke, with the fluid medium flowing through and around the yoke. The required power to send a maximum flux through the magnetic yoke is less than the required power in a conventional apparatus for performing electroporation.
    Type: Grant
    Filed: August 26, 2008
    Date of Patent: March 18, 2014
    Assignee: Board of Regents, The University of Texas System
    Inventor: Kent Davey
  • Publication number: 20140057337
    Abstract: A system and method are described for electroporating a sample that utilizes one or more sets of electrodes that are spaced apart in order to hold a surface tension constrained sample between the electrodes. The first electrode is connected to the lower body of the system while the second electrode is connected to the upper body. Both electrodes are connected to a pulse generator. Each electrode has a sample contact surface such that the first electrode and the second electrode may be positioned to hold a surface tension constrained sample between the two sample contact surfaces and the sample may receive a selected electric pulse.
    Type: Application
    Filed: February 12, 2013
    Publication date: February 27, 2014
    Applicant: APPLIED BIOYSYSTEMS, LLC
    Inventors: Richard JARVIS, Michael Byrom, Dimitriy Ovcharenko
  • Patent number: 8642306
    Abstract: A liquid sample flow containing living cells is irradiated with measurement laser light and the photo data of at least either scattering light or fluorescence that is generated by each of the living cells in the liquid sample flow due to the irradiation with the measurement laser light is acquired. Based on the photo data thus acquired, it is determined whether each of the cells assignable to the respective photo data is an unnecessary living cell or a target living cell. Based on the determination results, a pulse voltage is then applied exclusively to the living cells having been determined as unnecessary living cells so that the unnecessary living cells are damaged and killed.
    Type: Grant
    Filed: June 11, 2008
    Date of Patent: February 4, 2014
    Assignee: Mitsui Engineering & Shipbuilding Co., Ltd.
    Inventors: Shigeyuki Nakada, Noriaki Kimura, Akihide Ito, Kenji Yasuda
  • Patent number: 8642322
    Abstract: The present invention relates to methods for measuring an amount of an analyte using an electrochemical assay in a conduit comprising a sensor, wherein said sensor comprises an electrode having a surface layer of immobilized antibody that binds said analyte, and a counter/reference electrode disposed within said conduit. A solution comprising a substrate for said enzyme and at least one air segment contacts the sensor to remove unbound analyte and labeled antibody from a region of the sensor. The disclosed invention is adaptable to the point-of-care clinical diagnostic field, including use in accident sites, emergency rooms, surgery, nursing homes, intensive care units, and non-medical environments.
    Type: Grant
    Filed: April 12, 2013
    Date of Patent: February 4, 2014
    Assignee: Abbott Point of Care Inc.
    Inventors: Graham Davis, Imants Lauks, Chao Lin, Cary James Miller
  • Patent number: 8580544
    Abstract: The invention relates to an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material with the object of providing an apparatus for introducing a biological material, a method of introducing a biological material, and a magnetic support for introducing a biological material whereby a biological material can be efficiently introduced into a host. The invention comprises: one or more packing units in which a mixture solution containing a large number of magnetic supports carrying a biological material to be introduced into a host such as cells upon using, together with a large number of the hosts in a liquid is pooled; and an introduction treatment unit in which a magnetic force affecting the inside of the packing unit is controlled so as to move the magnetic supports relatively with respect to the host so that the biological material can be introduced into the host.
    Type: Grant
    Filed: October 16, 2003
    Date of Patent: November 12, 2013
    Assignees: Universal Bio Research Co. Ltd.
    Inventors: Hideji Tajima, Yoshiro Okami
  • Publication number: 20130260467
    Abstract: A molecular delivery device including a plurality of nanowires (e.g., Si NWs) coated with an electrically conductive layer. Also disclosed are methods for delivering a molecule by nanowire-mediated electroporation.
    Type: Application
    Filed: September 28, 2011
    Publication date: October 3, 2013
    Applicant: President and Fellows of Harvard
    Inventors: Hongkun Park, Jacob Robinson, Amy Sutton, Marsela Jorgolli, Alexander K. Shalek
  • Publication number: 20130230895
    Abstract: The invention relates to a method for subjecting adherent cells to at least one electric field, in which the electric field is generated by applying a voltage to at least two active electrodes 63, wherein at least three electrodes 63, 64 are provided, and wherein at least two electrodes 63, 64 are active electrodes 63 when the voltage is applied in order to generate a first electric field, and in which at least one second electric field is generated, wherein at least one of the two previously active electrodes 63 is a potential-free electrode 64 when the voltage is applied.
    Type: Application
    Filed: June 21, 2011
    Publication date: September 5, 2013
    Applicant: LONZA COLOGNE GMBH
    Inventors: Thomas Koblizek, Andreas Heinze, Timo Gleissner, Herbert Mueller-Hartmann, Andreas Wirth
  • Patent number: 8513019
    Abstract: A fluid containing cells and free genetic material is acoustically coupled to a propulsion surface of a diaphragm. A blast-receiving surface of the diaphragm is acoustically coupled to an explosion chamber in which an explosive material is disposed. An ignition system ignites the explosive material in the explosion chamber to create a blast wave. The diaphragm transfers momentum from the blast wave to the fluid containing cells and free genetic material sufficient to cause the cells to take up the free genetic material.
    Type: Grant
    Filed: July 27, 2012
    Date of Patent: August 20, 2013
    Assignee: Indian Institute of Science
    Inventor: Gopalan Jagadeesh
  • Patent number: 8513000
    Abstract: Transfecting biology cells with nucleic acid molecules (DNA, siRNA) is an essential prerequisite in elucidating how genes function in complex cellular context and how their activities could be modulated for therapeutic intervention. Traditionally studies are carried out on a low throughput gene-by-gene scale, which has created a huge bottleneck in functional genomic study and drug discovery. Development of high-throughput cell transfection technology will permit functional analysis of massive number of genes and how their activities could be modulated by chemical or biological entities inside cells. This invention describes design, construction of device and apparatus for high throughput effective cell transfection. Procedures and protocols for using the device and apparatus are also described in the application. Novel methods of using the device in cell-based assays are also disclosed.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: August 20, 2013
    Assignee: Rational Biotechnology, Inc.
    Inventor: Yong Huang
  • Publication number: 20130196441
    Abstract: Provided herein are the concept that “singularity-based configuration” electrodes design and method can produce in an ionic substance local high electric fields with low potential differences between electrodes. The singularity-based configuration described here includes: an anode electrode; a cathode electrode; and an insulator disposed between the anode electrode and the cathode electrode. The singularity-based electrode design concept refers to electrodes in which the anode and cathode are adjacent to each other, placed essentially co-planar and are separated by an insulator. The essentially co-planar anode/insulator/cathode configuration bound one surface of the volume of interest and produce desired electric fields locally, i.e., in the vicinity of the interface between the anode and cathode. In an ideal configuration, the interface dimension between the anode and the cathode tends to zero and becomes a point of singularity.
    Type: Application
    Filed: May 31, 2011
    Publication date: August 1, 2013
    Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Boris Rubinsky, Gregory D. Troszak
  • Patent number: 8465955
    Abstract: Described are methods for electrical treatment of biological cells, in particular using electrical field pulses, involving the steps: arrangement of the cells (1) on apertures (2) of a solid planar carrier element (3) which divides a measuring chamber (10) into two compartments (11, 12); and temporary formation of an electrical treatment field which permeates the cells, wherein an alternating-current impedance measurement takes place on the carrier element (3), and from the result of the alternating-current impedance measurement, a degree of coverage of the carrier element and/or healing of the cells after electrical treatment are/is acquired. Also described are devices for implementing the methods.
    Type: Grant
    Filed: February 22, 2008
    Date of Patent: June 18, 2013
    Assignee: Eppendorf AG
    Inventors: Ulrich Zimmermann, Vladimir Sukhorukov, Kurt Lucas
  • Patent number: 8460921
    Abstract: A neuronal network analysis plate having alternating rows of recording wells and amplifying wells. The recording wells contain a neural cell network and a series of electrodes for recording the action potential signals of the neurons. The electrodes are connected to amplifiers in adjacent amplifying wells. The close proximity of these amplifiers ideal because it permits the parallel, non-multiplexed recording of action potential signals from multiple different active nerve cell networks. The amplifiers in the amplifying wells can then be connected to external amplification equipment. The neuronal network analysis plate may be contained within a single commercially available 24 or 96 well plate. The neuronal network analysis plate can be used to detect and quantify pharmacological and toxicological responses of the neural cells to one or more agents in vitro.
    Type: Grant
    Filed: September 12, 2007
    Date of Patent: June 11, 2013
    Assignee: University of North Texas
    Inventor: Guenter W. Gross
  • Patent number: 8450098
    Abstract: The method disclosed herein, relates generally to introducing molecules such as biomolecules (e.g., nucleic acids) into a filamentous fungus. More specifically, the methods disclosed herein relate to introducing one or more nucleic acids into a filamentous fungus.
    Type: Grant
    Filed: May 21, 2008
    Date of Patent: May 28, 2013
    Assignee: Danisco US Inc.
    Inventors: Steven Kim, Andrei Miasnikov
  • Patent number: 8450102
    Abstract: A device 1 with at least one electrode 8 for generating an electrical field in a chamber 7 is disclosed. The device 1 comprises at least one input channel 6 for introducing a fluid into the chamber 7, and at least one output channel 11 for discharging the fluid from the chamber 7. Also disclosed is a method for stabilizing the flow of a fluid through a chamber 7 in which an electrical field is generated and which has at least one input channel 6 for introducing the fluid into the chamber and at least one output channel 11 for discharging the fluid from the chamber 8. To avoid undesirable backflow of the fluid due to gas formation, the average inside diameter of the input channel 6 of device 1 is smaller than the average inside diameter of the output channel 11.
    Type: Grant
    Filed: February 22, 2008
    Date of Patent: May 28, 2013
    Assignee: Lonza Cologne GmbH
    Inventors: Andreas Wirth, Michael Kazinski
  • Patent number: 8420363
    Abstract: A device for detecting cells and/or molecules on an electrode surface is disclosed. The device detects cells and/or molecules through measurement of impedance changes resulting from the cells and/or molecules. A disclosed embodiment of the device includes a substrate having two opposing ends along a longitudinal axis. A plurality of electrode arrays are positioned on the substrate. Each electrode array includes at least two electrodes, and each electrode is separated from at least one adjacent electrode in the electrode array by an expanse of non-conductive material. The electrode has a width at its widest point of more than about 1.5 and less than about 10 times the width of the expanse of non-conductive material. The device also includes electrically conductive traces extending substantially longitudinally to one of the two opposing ends of the substrate without intersecting another trace. Each trace is in electrical communication with at least one of the electrode arrays.
    Type: Grant
    Filed: December 1, 2008
    Date of Patent: April 16, 2013
    Assignee: Acea Biosciences, Inc.
    Inventors: Xiaobo Wang, Xiao Xu
  • Publication number: 20130089931
    Abstract: A microdevice for fusing cells including: a membrane with a plurality of pores having a diameter smaller than the smallest diameter among the first kind of cells and second kind of cells; a first chamber where the first cell is located and a second chamber where the second cell is located, wherein the membrane is disposed therebetween; a first electrode combined to the first chamber; a second electrode combined to the second chamber; and a power generator applying a voltage to the first and second electrodes. Accordingly, the first and second cells across the membrane may be arranged in a one-to-one manner between the first and second electrodes, and thus the first and second cells having different traits may be smoothly fused in a one-to-one manner when electric signals are sequentially applied thereto.
    Type: Application
    Filed: October 27, 2011
    Publication date: April 11, 2013
    Inventors: Sang-Woo JOO, Shizhi Qian, Ning Hu