Including Multiple Internal Compartments Or Baffles Patents (Class 435/288.2)
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Patent number: 11884908Abstract: This application relates to an automated three-dimensional (3D) cell culture system. The system includes a first body including a cell culture space in which a scaffold, on which cells are cultured, is provided and the cells are cultured thereon, and a medium storage space which is partitioned from the cell culture space by a first partition wall, in which a medium is provided and stored, and which surrounds the cell culture space, wherein the first partition wall has a drain hole to communicate the medium storage space with the cell culture space. The system also includes a communication pipe provided at the first partition wall to drain the medium of the cell culture space to the outside by a siphon action. The system further includes a second body including a medium recovery space located under the first body and recovering and externally draining the medium drained through the communication pipe.Type: GrantFiled: May 13, 2019Date of Patent: January 30, 2024Assignee: Danagreen Co., Ltd.Inventor: Seung Yon Ju
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Patent number: 11181447Abstract: In an apparatus for collecting aerosolised respirable particles of an inhalable medicinal formulation, aerosolised formulation is drawn pneumatically through a dose collection section comprising an inlet orifice (201) and an air-permeable filter (206), the filter being positioned opposed to said orifice, and extending across the pathway (4) for filtering the pneumatic flow so as to retain particulate material therein on the filter, and the orifice (201) being so dimensioned and configured that it has an unimpeded area that is no less than 75% of the area of the filter (201) on which the dose will be collected. In a method using the apparatus, particles (209, 210) collected on the filter may optionally be subjected to a dissolution test. A good correlation is obtainable between in vitro and in vivo doses with improved independence of loading.Type: GrantFiled: June 17, 2020Date of Patent: November 23, 2021Assignee: NANOPHARM LIMITEDInventors: Robert Price, Jagdeep Shur
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Patent number: 10884010Abstract: A diffusion cell includes: a donor chamber having bottom; a receptor chamber below and in fluid communication with the donor chamber, and having upper and lower ends; a membrane between and in contact with the bottom of the donor chamber and upper end of the receptor chamber, and adapted to diffuse some of the media in a liquid from the donor chamber to the receptor chamber; a conduit having a first port near the lower end of the receptor chamber and a second port above the first port and near the upper end of the receptor chamber; and a bubble trap in fluid communication with the upper end of the receptor chamber and having a third port higher than the second port; wherein circulation of a flow of fluid through the conduit and the receptor chamber removes bubbles from underneath the membrane and transports bubbles to the bubble trap.Type: GrantFiled: May 22, 2020Date of Patent: January 5, 2021Inventor: Andrew Wilt
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Patent number: 9909162Abstract: Described herein are methods and apparatus for rapid detection of microorganisms in biological samples (e.g. blood) for analysis to determine the presence or absence of infectious microorganisms in the samples. The apparatus includes a cartridge with a lid and a tray, a mechanism for isolating a bulk sample into multiple smaller samples, and a sensor disposed on the tray to determine the presence or absence of microorganisms. The cartridge lid includes projections that, in a first position, allow for sample to distribute evenly in the cartridge tray and, in a second position, isolate the sample into multiple smaller volume samples. The apparatus and method shorten the time-to-detection of a microorganism in a sample and reduce the steps required from sample collection to microorganism detection.Type: GrantFiled: March 11, 2013Date of Patent: March 6, 2018Assignee: Becton, Dickinson and CompanyInventor: Ming-Hsiung Yeh
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Patent number: 8999729Abstract: A device for conducting an agglutination assay comprising several reaction vessels, each reaction vessel comprising an upper chamber having an opening for accepting reactants and/or a sample; and a lower chamber comprising an end in communication with the upper chamber for receiving fluids from the upper chamber, a closed end opposite to the end, and a matrix for separating agglutinates from non-agglutinates; wherein the device further comprises a rotating support able to rotate around an axis and holding pivotally the reaction vessels in a way to allow the reaction vessels to pivot about an axis essentially perpendicular to the rotation axis of the support when the latter is rotated, such that the fluids remain in the upper chamber when the support is not rotated, and can flow from the upper chamber to the lower chamber and into the matrix when the support is rotated.Type: GrantFiled: June 21, 2011Date of Patent: April 7, 2015Assignee: Symbion Medical Systems SarlInventors: Isabelle Delafosse, Fabrice Ummel, Antonio Yanez
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Patent number: 8993241Abstract: Provided is a device and a method whereby plural kinds of reaction operations and washing operations can be conducted in parallel without washing or replacing an instrument used in transferring a solution in each operation. A reaction device having a plurality of projecting barriers provided in a line on one surface of a substrate. The projecting barrier has a cutoff portion and an inner space capable of holding a droplet and having a contact angle to pure water of from 90 to 150 degrees. A reaction method using the reaction device wherein a substance immobilized on magnetic beads is sequentially transferred in and between droplets of a solution containing a surface tension reducing agent that are held in the spaces for holding a droplet by means of a magnet located on the opposite surface of the substrate to thereby conduct reactions and washings.Type: GrantFiled: January 16, 2009Date of Patent: March 31, 2015Assignee: National University Corporation University of ToyamaInventors: Masaharu Isobe, Nobuyuki Kurosawa
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Patent number: 8969073Abstract: The invention describes an appliance and a method, with the help of which specific bio-particles, but also dissolved bio-molecules can be recognized in and separated from fluids making use of suitable carriers and known immobilization methods. The appliance can be used both discontinuously and also for direct and continuous treatment of fluids. Fields of application of the invention are animals, bio-technology (including biological research) and medicinal diagnostics. Areas of application of the invention comprise, among others, therapy of humans, in particular direct treatment of blood.Type: GrantFiled: January 30, 2007Date of Patent: March 3, 2015Assignee: pluriSelect GmbHInventor: Hans Werner Heinrich
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Patent number: 8945481Abstract: Exemplary embodiments provide microfludic devices and methods for their use. The microfluidic device can include an array of M×N reaction sites formed by intersecting a first and second plurality of fluid channels of a flow layer. The flow layer can have a matrix design and/or a blind channel design to analyze a large number of samples under a limited number of conditions. The microfluidic device can also include a control layer including a valve system for regulating solution flow through fluid channels. In addition, by aligning the control layer with the fluid channels, the detection of the microfluidic devices, e.g., optical signal collection, can be improved by piping lights to/from the reaction sites. In an exemplary embodiment, guard channels can be included in the microfluidic device for thermal cycling and/or reducing evaporation from the reaction sites.Type: GrantFiled: June 24, 2008Date of Patent: February 3, 2015Assignee: Applied Biosystems, LLCInventors: Mark F. Oldham, Kenneth J. Livak, Jason E. Babcoke, H. Pin Kao, Stephen J. Gunstream, Kevin S. Bodner, Douglas P. Greiner, Nigel P. Beard, Dar Bahatt
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Patent number: 8940524Abstract: We describe a thermal cycler comprising a Peltier-type thermoelectric element used for cooling a sample block, and a non-Peltier-type heating device for heating the sample block. The cycler also includes a heat sink connected to the Peltier-type element by a heat pipe, which permits thermal energy to transfer from the Peltier-type element to the heat sink. This configuration operates more efficiently than conventional thermal cyclers which use Peltier-type elements for heating and cooling, and allows a more rapid cycling time as well as operation in a wider range of ambient temperatures. Certain embodiments utilise the Peltier-type element as a thermal gate to reduce thermal loss during heating when the Peltier-type element is switched off.Type: GrantFiled: September 22, 2011Date of Patent: January 27, 2015Assignee: Epistem LimitedInventor: Ben Cobb
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Patent number: 8932544Abstract: Disclosed are a method and a device for feeding and/or discharging fluids in microreactor arrays through one or several fluid ducts that extend into each individual microreactor and can be individually controlled and regulated, in order to control, regulate, influence, and/or verify processes. The fed or discharged amounts of fluid are introduced into or discharged from the volume of the reaction liquid during a continuous shaking process and are evenly mixed as a result of the shaking process. The continuous shaking process causes sufficient mass transfer and thorough mixing of the reaction partners or fluids while the reaction is not limited or restricted by the mass transfer conditions.Type: GrantFiled: June 28, 2007Date of Patent: January 13, 2015Assignee: m2p-labs GmbHInventors: Carsten Mueller, Ingo Klammer, Frank Kensy, Jochen Buechs, Mirko Hofmann, Andreas Buchenauer, Uwe Schnakenberg, Wilfried Mokwa
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Patent number: 8883095Abstract: An analysis cartridge and an analysis system thereof are disclosed. The analysis cartridge comprises a cartridge body, a liquid storage box and a sealing film. The cartridge body has an accommodation portion. The liquid storage box is disposed within the accommodation portion and has a liquid storage tank and a pillar receiving hole. The sealing film covers the pillar receiving hole and seals an opening of the liquid storage tank.Type: GrantFiled: January 14, 2013Date of Patent: November 11, 2014Assignee: Lite-On Technology CorporationInventors: Cheng-Chang Lai, Fu-Chun Huang, Yuh-Jiuan Lin, Timothy Z. Liu
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Patent number: 8859264Abstract: A phantom device for in-vitro validation of radiation procedures under motion influence in consideration of an effective biological dose includes a phantom having a first biological detector with a first biological sample. The first biological sample includes a plurality of culturing and irradiation elements. Each of the culturing and irradiation elements are provided with a respective biological sub-sample so that the first biological detector is configured as a spatially resolving biological detector. A first motion device is configured to move the first biological detector so as to simulate a motion of a target volume.Type: GrantFiled: July 29, 2009Date of Patent: October 14, 2014Assignee: GSI Helmholtzzentrum fuer Schwerionenforschung GmbHInventors: Christoph Bert, Eike Rietzel, Alexander Gemmel
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Patent number: 8828654Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.Type: GrantFiled: July 8, 2011Date of Patent: September 9, 2014Assignee: Gen-Probe IncorporatedInventors: Norman C. Nelson, Gianluca Roma
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Patent number: 8821814Abstract: A microfluidic device including a platform and a cartridge is disclosed. The platform includes a chamber containing a fluid. The reagent cartridge is mounted to the platform. and contains a solid reagent for detecting material contained in the fluid.Type: GrantFiled: June 30, 2009Date of Patent: September 2, 2014Assignee: Samsung Electronics Co., Ltd.Inventors: Yoonkyoung Cho, Dogyoon Kim, Beomseok Lee, Jongmyeon Park, Hyunmin Kim, Yangui Lee, Jong Gun Lee
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Patent number: 8815178Abstract: The invention relates to a device and method for non-invasive detection of an analyte in a fluid sample. In one embodiment, the device comprises: a collection chamber containing an absorbent hydrogel material; a fluidic channel connected to the collection chamber; a sensing chamber connected to the fluidic channel, wherein the device is comprised of a compressible housing that allows transfer of fluid collected by the collection chamber to be transferred to be extracted and withdrawn to the sensing chamber upon compression of the device, wherein the sensing chamber contains a material that specifically detects the analyte and wherein the sensing chamber is operably linked to a processor containing a potentiostat that allows detection of the analyte using electrochemical sensing.Type: GrantFiled: March 25, 2010Date of Patent: August 26, 2014Assignee: Arizona Board of Regents on Behalf of Arizona State UniversityInventors: Daniel Bishop, Jeffrey La Belle
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Patent number: 8765367Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.Type: GrantFiled: December 12, 2008Date of Patent: July 1, 2014Assignees: Gen-Probe Incorporated, Qualigen, Inc.Inventors: Scott S. Breidenthal, Sara H. Fan, Richard S. Lee, Norman C. Nelson, Matthew J. Scott, Jason A. Taylor
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Patent number: 8741630Abstract: The invention relates to methods of detecting a target analyte in a biological sample using composite microsphere arrays having first and second assay locations. Preferred target analytes include nucleic acid, and more specifically, nucleic acid having one or more single nucleotide polymorphisms (SNPs).Type: GrantFiled: December 17, 2010Date of Patent: June 3, 2014Assignee: Illumina, Inc.Inventors: Todd Dickinson, Kenneth D. Coblentz, Edward Carlson
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Patent number: 8735055Abstract: A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample.Type: GrantFiled: December 12, 2008Date of Patent: May 27, 2014Assignees: Gen-Probe Incorporated, Qualigen, Inc.Inventors: Scott S. Breidenthal, Sara H. Fan, Richard S. Lee, Norman C. Nelson, Matthew J. Scott, Jason A. Taylor
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Patent number: 8709787Abstract: The present technology provides for a microfluidic substrate configured to carry out PCR on a number of polynucleotide-containing samples in parallel. The substrate can be a single-layer substrate in a microfluidic cartridge. Also provided are a method of making a microfluidic cartridge comprising such a substrate. Still further disclosed are a microfluidic valve suitable for use in isolating a PCR chamber in a microfluidic substrate, and a method of making such a valve.Type: GrantFiled: November 14, 2007Date of Patent: April 29, 2014Assignee: Handylab, Inc.Inventor: Kalyan Handique
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Patent number: 8685708Abstract: The invention provides a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA.Type: GrantFiled: April 12, 2013Date of Patent: April 1, 2014Assignee: PathoGenetix, Inc.Inventors: John L. Harris, Adrian Mark Thomas West, Dirk Peter Ten Broeck, Ekaterina Protozanova
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Patent number: 8652828Abstract: Nanochannel arrays that enable high-throughput macromolecular analysis are disclosed. Also disclosed are methods of preparing nanochannel arrays and nanofluidic chips. Methods of analyzing macromolecules, such as entire strands of genomic DNA, are also disclosed, as well as systems for carrying out these methods.Type: GrantFiled: October 30, 2008Date of Patent: February 18, 2014Assignee: The Trustees of Princeton UniversityInventors: Robert H Austin, Zhaoning Yu, Jonas O Tegenfeldt, Stephen Y Chou, Han Cao
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Patent number: 8603806Abstract: The methods generally relate to three-dimensional culturing of cells with the steps of seeding a cell support structure in a device with at least one vessel for cell culture having a bottom, at least one wall and a cell support structure for three-dimensional cell culture. The cell support structure is inside the at least one vessel and the device is configured such that assays can be performed. Devices for performing cell culture and assays are also provided. It will be understood that the devices can have many different suitable configurations. In general, the devices are selected such that they can be used with suitable radiation based assays.Type: GrantFiled: November 2, 2006Date of Patent: December 10, 2013Assignee: The Ohio State Universtiy Research FoundationInventors: Shang-Tian Yang, Xudong Zhang, Yuan Wen
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Patent number: 8597938Abstract: Provided are methods and oligonucleotides useful as primers and templates for internal controls designed for use in Real Time Reverse Transcriptase Polymerase Chain Reactions. Use of the present methods and oligonucleotides allows validation of assay parameters and of the results that an assay set.Type: GrantFiled: October 10, 2008Date of Patent: December 3, 2013Assignee: QIAGEN Sciences LLCInventors: Jingping Yang, Li Shen
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Patent number: 8585982Abstract: A field colorimetric test device includes a housing having at least one interior compartment. A sampling device is configured to be associated with a distal end of the housing. A selection device is attached to a proximal end of the housing.Type: GrantFiled: June 12, 2012Date of Patent: November 19, 2013Assignee: The United States of America as represented by the Secretary of the NavyInventors: Roxanne L. Quintana, Martin W. Krauss, Alvin L. Quintana
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Patent number: 8551714Abstract: Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.Type: GrantFiled: February 6, 2012Date of Patent: October 8, 2013Assignee: IntegenX Inc.Inventors: Stevan Bogdan Jovanovich, Iuliu Ioan Blaga
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Patent number: 8492140Abstract: The invention provides systems, modules, bioreactor and methods for the automated culture, proliferation, differentiation, production and maintenance of tissue engineered products. In one aspect is an automated tissue engineering system comprising a housing, at least one bioreactor supported by the housing, the bioreactor facilitating physiological cellular functions and/or the generation of one or more tissue constructs from cell and/or tissue sources. A fluid containment system is supported by the housing and is in fluid communication with the bioreactor. One or more sensors are associated with one or more of the housing, bioreactor or fluid containment system for monitoring parameters related to the physiological cellular functions and/or generation of tissue constructs; and a microprocessor linked to one or more of the sensors. The systems, methods and products of the invention find use in various clinical and laboratory settings.Type: GrantFiled: April 8, 2003Date of Patent: July 23, 2013Assignee: Octane Biotech Inc.Inventors: Timothy J. N. Smith, Martin R. Pecaric, Sydney J. Pugh, Rupert Hagg, Roberto Tommasini, Yves Larcher, Lowell D. Misner
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Patent number: 8445265Abstract: The invention relates to a reaction vessel and a reaction controller, wherein temperature control of a liquid stored within the vessel can be performed with a high accuracy and faithful responsiveness. The reaction container and the reaction controller comprise one or a plurality of reaction chambers in which liquid is storable, and a wall that surrounds the reaction chamber, and the entirety or a part of the wall is formed by a temperature raising and lowering body that can raise or lower the temperature thereof according to a signal from an instructing part provided outside.Type: GrantFiled: October 5, 2005Date of Patent: May 21, 2013Assignee: Universal Bio Research Co., Ltd.Inventors: Hideji Tajima, Yoshinao Hirahara, Osamu Segawa
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Patent number: 8425864Abstract: A container assembly for storing, treating, transporting and stabilizing a biological sample includes a container, a cap and a sample holder removably received in the container. The sample holder can be a platform-like device dimensioned to be supported on a ledge formed in the side wall of the container. The sample holder includes a central cavity for receiving the sample and immersing the sample in the stabilizing agent in the container. In another embodiment, the sample holder has a closure member for closing the open top end of the cavity. The container includes a liquid reagent in an amount sufficient to treat the biological sample. The biological sample is retained in a predetermined containment area of the container to maintain the biological sample immersed in the reagent without regard to the orientation of the container.Type: GrantFiled: November 14, 2011Date of Patent: April 23, 2013Assignee: Becton, Dickinson and CompanyInventors: Bruce C. Haywood, Jamieson William Maclean Crawford, Bradley M. Wilkinson
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Patent number: 8415140Abstract: An analysis device includes: a separation cavity 18 for separating a test liquid into a solution component and a solid component by using a centrifugal force; a higher specific gravity component quantitative cavity 23 for holding a portion of the separated solid component which has been transferred; a sample solution overflow cavity 22 arranged between the higher specific gravity component quantitative cavity 23 and the separation cavity 18 and connected to a connecting channel 21 for transporting the sample liquid from the separation cavity 18; and a capillary cavity 19 formed in the separation cavity 18 for temporarily holding a separated solution component (blood plasma) in the separation cavity 18. A blood plasma component 57a remaining in the separation cavity 18 is trapped by the capillary cavity 19.Type: GrantFiled: October 3, 2008Date of Patent: April 9, 2013Assignee: Panasonic CorporationInventors: Hiroshi Saiki, Takashi Miki, Takuya Suzuki
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Patent number: 8394625Abstract: This invention generally relates to an integrated ‘lab-on-a-Pipette’™ which will provide sample-to-answer single cell genetic diagnosis for preimplantation genetic diagnosis (PGD) and other forms of single cell analysis (SCA). SCA is a quickly growing field with substantial impact in prenatal testing, cancer biopsies, diabetes, stem cell research, and our overall understanding of heterogeneity in biology. However, single cell genetic analysis is challenging, inaccurate, and in many cases impossible, due to the small amount of sample (5 pg), and difficulties in handling small sample volumes (50-100 pL). The ‘lab-on-a-pipette’ device integrates a microaspiration tip with microfluidic analysis components to conduct in-situ, real-time single cell genetic diagnosis in a single device. The microaspiration tip extracts and encapsulate a cell into an ultra-low volume plug (˜300 pL).Type: GrantFiled: May 2, 2011Date of Patent: March 12, 2013Inventors: Angelo Gaitas, Amar Basu
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Patent number: 8383422Abstract: The present invention provides an immunochromatographic device, which contains the following (a) and (b): (a) a first device part holding a first insoluble carrier used for developing a complex formed with an analyte and a labeling substance comprising a metal labeled with a first binding substance that can bind to the analyte and capturing the analyte and the labeling substance at a reaction portion containing a second binding substance that can bind to the analyte, and (b) a second device part holding a second insoluble carrier used for developing a liquid and a third insoluble carrier used for absorbing a liquid, in such a way that the first insoluble carrier does not come into contact with the second insoluble carrier and the third insoluble carrier.Type: GrantFiled: May 26, 2009Date of Patent: February 26, 2013Assignee: FUJIFILM CorporationInventors: Junichi Katada, Hideyuki Karaki, Takayoshi Oyamada
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Patent number: 8383421Abstract: An object of the present invention is to provide a cartridge for automatic measurement used in an automatic measuring device, capable of automatically performing measurement including heat treatment of a sample, and a measuring device using the cartridge. The present invention relates to a cartridge for use in measuring a component to be measured contained in a sample, comprising: at least a heat-treatment well for performing heat treatment of the sample; and a reaction well for reacting the component to be measured in the sample with a material specifically reacting therewith.Type: GrantFiled: January 17, 2006Date of Patent: February 26, 2013Assignee: Mitsubishi Chemical Medience CorporationInventors: Atsushi Yanagida, Takashi Kurihara, Hiroyuki Yokoi, Atsushi Koyata, Yoshikazu Okamura, Daishi Miyamoto
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Patent number: 8383393Abstract: A titer plate and a method for detecting an analyte, and the use thereof are disclosed. According to at least one embodiment of the invention, it is proposed that a plurality of depressions and a biochip of the titer plate sposed adjacent thereto be surrounded by a wall in order to effectively prevent sample contamination when there is a high degree of spatial integration.Type: GrantFiled: May 22, 2009Date of Patent: February 26, 2013Assignee: Siemens AktiengesellschaftInventors: Walter Gumbrecht, Peter Paulicka
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Patent number: 8372343Abstract: Embodiments of the present invention relate to multiple coagulation test cartridges and methods of using such cartridges. In one embodiment the cartridge is a disposable single-use cartridge for use in evaluating blood clotting. The cartridge includes multiple containers, such as tubes, each of which includes one or more coagulation affecting substances. The containers can be pre-filled with substances in amounts suitable for use with a single patient's blood sample. The cartridge may include one or more containers, each of which has multiple sections, or volumes, each section storing a different coagulation affecting substance. In another embodiment the cartridge is used in a method for determining at least one appropriate coagulation affecting substance for modifying a patient's coagulation status using a multiple coagulation test system.Type: GrantFiled: December 7, 2007Date of Patent: February 12, 2013Inventor: Sheldon Goldstein
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Patent number: 8372658Abstract: A chemical analytic apparatus of the present invention is the one which proposes that a miniaturization, a making low-cost and portability are possible and also the operation of each process of separation, concentration and dilution of specimen is possible, and, which includes: an introduction means (S1) that introduces a droplet to which magnetic ultrafine particles are mixed into another liquid that differs from the droplet while maintaining a single droplet; a conveyance means by which the droplet that includes the magnetic particles is conveyed in another liquid of the introduction means by applying magnetic field externally to the magnetic ultrafine particles; and processing means (S2 to S6) by which operations for processing of chemical analysis are performed one by one in the process in which the droplet to which the magnetic ultrafine particles are mixed is conveyed by the conveyance means.Type: GrantFiled: February 7, 2012Date of Patent: February 12, 2013Assignee: Japan Science and Technology AgencyInventors: Mitsuhiro Shikida, Kazuo Sato, Hiroyuki Honda, Kohta Inouchi
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Patent number: 8349279Abstract: A samples storage system for pharmaceutical development in which the usable volumes of ultramicrotubes (384 tubes) are increased and smooth insertion and extraction of ultramicrotubes is possible irrespective of the positions of the ultramicrotubes. The samples storage system includes tubes in which samples are sealed and a storage rack for vertically accommodating a plurality of the tubes in a grid pattern. Each tube is of a rectangular hollow tubular cross-section and the intersect is tapered toward the bottom portion of the tube. Corner portions of the outer four side surfaces of the tubes are chamfered. The storage rack has engagement partition walls forming open-ended sections in a grid pattern inside the rack frame. The height of the walls is smaller than the length of the tube, and tube-supporting pins project vertically from the intersections of the grid.Type: GrantFiled: July 16, 2010Date of Patent: January 8, 2013Assignee: Tsubakimoto Chain Co.Inventors: Hiroyuki Taike, Kazuhiro Tsutsumi, Hisao Nishii, Nobuaki Matsumoto, Yukio Ueyama
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Patent number: 8323953Abstract: A device for the detection of micro particles that can be marked by probes or antibodies capable of being detected by radiation has a filter, a supply system, and a detection system. Fluid to be examined is passed over a filter to filter out the micro particles and to perform the marking steps by supplying corresponding marking substances to the filter.Type: GrantFiled: April 29, 2008Date of Patent: December 4, 2012Assignee: EADS Deutschland GmbHInventors: Alois Friedberger, Ulrich Reidt, Gerhard Müller
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Patent number: 8318479Abstract: A system has been constructed that recapitulate the features of a capillary bed through normal human tissue. The system facilitates perfusion of three-dimensional (3D) cell monocultures and heterotypic cell co-cultures at the length scale of the capillary bed. A major feature is that the system can be utilized within a “multiwell plate” format amenable to high-throughput assays compatible with the type of robotics commonly used in pharmaceutical development. The system provides a means to conduct assays for toxicology and metabolism and as a model for human diseases such as hepatic diseases, including hepatitis, exposure-related pathologies, and cancer. Cancer applications include primary liver cancer as well as metastases. The system can also be used as a means of testing gene therapy approaches for treating disease and inborn genetic defects.Type: GrantFiled: May 19, 2005Date of Patent: November 27, 2012Assignees: Massachusetts Institute of Technology, Univeristy of Pittsburgh—Of the Commonwealth System of Higher EducationInventors: Karel Domansky, Linda G. Griffith, Steven R. Tannenbaum, Alan Wells, Samuel Walker Inman
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Patent number: 8313941Abstract: Microfluidic devices having active features such as valves, peristaltic pumps, and mixing portions are fabricated to have a thin elastomeric membrane over the active features. The active features are activated by a tactile actuator external to the membrane, for example, a commercial Braille display. The display may be computer controlled, for example by simple text editor software, to activate individual Braille protrusions or a plurality of protrusions to actuate the active portions of the microfluidic device. Integral devices can incorporate the tactile actuators in a single device, but still external to the membrane.Type: GrantFiled: May 20, 2010Date of Patent: November 20, 2012Assignee: The Regents of The University of MichiganInventors: Shuichi Takayama, Xiaoyue Zhu, Wei Gu, Gary Daniel Smith, Yunseok Heo, Brenda S. Cho, Nobuyuki Futai
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Patent number: 8278091Abstract: Provided is an assay device and kit for detecting the presence or amount of an analyte of interest.Type: GrantFiled: December 18, 2007Date of Patent: October 2, 2012Assignee: Alere Switzerland GmbHInventors: Paul Rutter, Chris Jones, Andrew Ledgeway, James Gani, Bryan Tissington
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Patent number: 8273312Abstract: A liquid testing assembly for testing a liquid, the assembly comprising a test vessel and a stopper adapted to fit into a free end of the vessel. The stopper substantially hermetically seals the test vessel from the ambient. Further, the assembly includes a support coated with one or more identifying materials for identifying one or more constituents of the liquid. The support is fixed in the stopper and/or the vessel and extends into its interior for a predetermined distance. The liquid testing assembly when assembled is pre-evacuated to a predetermined vacuum sufficient to draw a predetermined volume of liquid to be sampled into the test vessel. The predetermined volume is of such an amount that it wets the one or more identifying materials ensuring identification of one or more constituents present in the liquid. A kit employing the liquid testing assembly is also discussed.Type: GrantFiled: January 22, 2008Date of Patent: September 25, 2012Assignee: Association for Public HealthInventors: Gadi Porat, Joel Stern, Boaz Rimon, Yoram Cohen
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Patent number: 8268611Abstract: The invention relates to a microtiter plate and use thereof for conducting fermentation under fed-batch conditions. In order to produce a microtiter plate which permits screening under fed-batch conditions, the invention proposes that the cavities (2) of the microtiter plate according to the invention be filled with a culturing fluid and nutrient solution and be designed in such a way that each of the cavities (2) of the microtiter plate which is filled with nutrient solution is connected by a channel (4) to at least one other further cavity (3) of the microtiter plate which is filled with a culturing fluid. A diffusion barrier (13) arranged in the material permeable channel (4) controls the kinetics of the material transfer of nutrients from the cavity containing the nutrient solution to the cavities containing the culturing fluid.Type: GrantFiled: May 21, 2007Date of Patent: September 18, 2012Assignee: RWTH AachenInventor: Jochen Büchs
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Patent number: 8263387Abstract: The invention relates generally to fluid processing and, in particular aspects, processing fluids for detection, selection, trapping and/or sorting of particulate moieties. Sheath flow devices described allow isolation of target species from fluid samples while avoiding non-specific binding of unwanted species to the surfaces of the separation device. Biological fluid processing, detection, sorting or selection of cells, proteins, and nucleic acids is described. The invention finds particular use in diagnostic settings, analyzing a patient's medical condition, monitoring and/or adjusting a therapeutic regimen and producing cell based products.Type: GrantFiled: June 10, 2010Date of Patent: September 11, 2012Assignee: Cynvenio Biosystems, Inc.Inventors: Paul Pagano, Yanting Zhang, Jiangrong Karen Qian, Hyongsok Tom Soh, Paul W. Dempsey, Andre′ De Fusco
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Patent number: 8232084Abstract: A device for measuring an extracellular potential of a test cell includes a substrate having a well formed in a first surface thereof and a first trap hole formed therein. The well has a bottom. The first trap hole includes a first opening formed in the bottom of the well and extending toward a second face of the substrate, a first hollow section communicating with the first opening via a first connecting portion, and a second opening extending reaching the second surface and communicating with the first hollow section via a second connecting portion. The first connecting portion has a diameter smaller than a maximum diameter of the first hollow section, greater than a diameter of the second connecting portion, and smaller than a diameter of the test cell. The device can retain the test cell securely and accept chemicals and the test cell to be put into the device easily.Type: GrantFiled: October 3, 2008Date of Patent: July 31, 2012Assignee: Panasonic CorporationInventors: Masaya Nakatani, Hiroaki Oka, Fumiaki Emoto
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Patent number: 8216826Abstract: A fluid analyzing apparatus. The fluid analyzing apparatus sequentially or simultaneously detects and analyzes a multiplex fluid sample with suitable analyzing elements. Meanwhile, the fluid analyzing apparatus may be disassembled to a first unit, a second unit and a third unit, such that the analyzing elements therein are easily disposed and replaced. The fluid analyzing apparatus analyzes and detects the multiplex fluid sample by allowing the multiplex fluid sample to sequentially or simultaneously flow through a plurality of target chambers. The fluid analyzing apparatus sequentially or simultaneously transports the multiplex fluid sample to the target chambers, thereby enhancing the speed and analysis thereof.Type: GrantFiled: October 14, 2008Date of Patent: July 10, 2012Assignee: Industrial Technology Research InstituteInventors: Kun Feng Lee, Pei-Shin Jiang, Yuh-Jiuan Lin
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Patent number: 8211367Abstract: A method of separating a cell-containing sample into a substantially cell-depleted portion, and a cell-containing portion comprising at least one of a stem cell, a lymphocyte, and a leukocyte comprises a step in which the sample is received in a vessel with at least one flexible wall. In another step, an additive and particles are added to the sample, wherein the additive substantially binds to the at least one of the stem cell, lymphocyte, and leukocyte, and the particles and wherein the particles substantially bind to the at least one of the stem cell, lymphocyte, and leukocyte, and the additive, thereby producing a cell-containing network. In a further step, the network is separated from the substantially cell-depleted portion by applying a magnetic force.Type: GrantFiled: January 18, 2011Date of Patent: July 3, 2012Inventors: James Wyatt, Matthew Scott, Vijay Mahant
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Patent number: 8206918Abstract: The present invention relates to systems, devices, and methods for performing biological reactions. In particular, the present invention relates to the use of lipophilic, water immiscible, or hydrophobic barriers in sample separation, purification, modification, and analysis processes.Type: GrantFiled: June 16, 2011Date of Patent: June 26, 2012Assignee: Northwestern UniversityInventors: David M. Kelso, Kunal Sur, Zaheer Parpia
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Patent number: 8183033Abstract: The invention relates to methods for preparing and performing quantitative PCR analyses, a new sealing device and a new use. According to the invention, a sample vessel containing the samples to be analyzed is sealed by placing a planar sealing device on the vessel to cover the samples and applying pressure on the sealing device in order to deform the sealing device so as to form a light-refracting geometry individually for the samples to be analyzed. The invention offers a convenient way of sealing the vessel and forming analysis-improving optical lenses over the samples simultaneously.Type: GrantFiled: March 21, 2008Date of Patent: May 22, 2012Assignee: Bioinnovations OyInventor: Bruce R Turner
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Patent number: 8163536Abstract: A device for preparing an undifferentiated cell, the device comprises means for contacting a more committed cell with an agent that causes the more committed cell to retrodifferentiate into an undifferentiated cell.Type: GrantFiled: May 9, 2001Date of Patent: April 24, 2012Assignee: Tristem Trading (Cyprus) LimitedInventor: Ilham Mohamed Saleh Saeed Abuljadayel
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Patent number: 8163537Abstract: A nested permeable support device is described herein that can be used to perform various experiments to test new therapeutic compounds (new chemical entities). In one application, the nested permeable support device is used to perform a first pass assay to determine the bioavailability of a new chemical entity following absorption through the digestive tract and metabolism by the liver.Type: GrantFiled: April 8, 2009Date of Patent: April 24, 2012Assignee: Corning IncorporatedInventors: Gregory R. Martin, Allison J. Tanner