Abstract: The present disclosure provides automated modules and instruments for improved detection of nuclease genome editing of live cells. The disclosure provides improved modules—including high throughput modules—for screening cells that have been subjected to editing and identifying and selecting cells that have been properly edited.

Abstract: A microbiological testing device for testing a liquid to be analysed that is liable to contain at least one microorganism, includes a closed inner space, a microbiological filtration member and an inlet port. The device has a nutritive layer in contact with the filtration member, and in that, in a configuration for providing the device an open/close member of the inlet port is in a closed state; the absolute gas pressure inside the closed inner space is strictly less than the standard atmospheric pressure, such that the device is able to create suction through the inlet port during a first opening of the open/close member.

Abstract: An exemplary mobile impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. The mobile cytometer may be controlled by a computer (e.g., smartphone) application. Calibration of the portable device may be performed using a component of known impedance value. With the developed portable flow cytometer, analysis may be performed on two sickle cell samples and a healthy cell sample. The acquired results may subsequently be analyzed to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals may be observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.

Abstract: A gas detecting module is disclosed. A gas-inlet concave and a gas-outlet concave are formed on a sidewall of a base. A gas-inlet-groove region and a gas-outlet-groove region are formed on a surface of the base. The gas-inlet concave is in communication with a gas-inlet groove of the gas-inlet-groove region, and the gas-outlet concave is in communication a gas-outlet groove of the gas-outlet-groove region. The gas-inlet-groove region and the gas-outlet-groove region are covered by a thin film to achieve the effectiveness of laterally inhaling and discharging out gas relative to the gas detecting module.

Abstract: A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel.

Abstract: A reaction processing vessel includes: a substrate; a channel for a sample to move that is formed on the substrate; a first air communication port and a second air communication port provided at respective ends of the channel; and a thermal cycle region for applying a thermal cycle to the sample that is formed between the first air communication port and the second air communication port in the channel. The channel includes a first branch channel and a second branch channel between the thermal cycle region and the first air communication port.

Abstract: A cell area extraction unit (241) extracts a cell area in a phase image that is created based on a hologram obtained by in-line holographic microscope (IHM). A background value acquisition unit (242) obtains a background value from phase values at a plurality of positions outside the cell area. An intracellular phase value acquisition unit (243) averages a plurality of phase values on a sampling line set at a position close to the periphery of a cell, while avoiding a central portion in which the phase value may be lowered in the cell area, to obtain an intracellular phase value. A phase change amount calculation unit (244) obtains the difference between the intracellular phase value and the background value. A phase change amount determination unit (245) compares the value of the difference with thresholds in two levels to determine whether the cell is in an undifferentiated state or an undifferentiation deviant state.

Abstract: The present disclosure relates to providing personalized prediction and prevention of various types of medical events (e.g., emergency department visits, hospital admissions, complications) using machine-learning algorithms. An exemplary method comprises: obtaining a plurality of feature values of the patient; providing the plurality of feature values to a set of one or more trained machine-learning models to obtain: a first probabilistic value indicating a likelihood of a future medical event, a second probabilistic value indicating a likelihood of a reason for the future medical event, a third probabilistic value indicating a likelihood that the future medical event can be prevented, displaying, on the display, a risk value of the future medical event based on the first probabilistic value, a reason of the future medical event based on the second probabilistic value, an interceptability value of the future medical event based on the third probabilistic value.

Abstract: The invention relates to the field of biotechnology and pharmaceutics. Proposed is a method for ultra-high performance screening of biological objects which is based on microfluidic generation of droplets of a biocompatible water-in-oil-in-water double emulsion, and also a method for producing a monodisperse biocompatible water-in-oil-in-water double emulsion. The invention can be used in diagnosing conditions and diseases in mammals, as well as for investigating biological objects.

Abstract: This disclosure provides methods and systems for classifying biological particles, e.g., blood cells, microbes, circulating tumor cells (CTCs). Using impedance flow cytometry, such as multi-frequency impedance cytometry, in conjunction with supervised machine learning, the disclosed methods and systems demonstrated improved accuracy in classifying biological particles.

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

Abstract: A microfabricated device having at least one gas-entrapping feature formed therein in a configuration that entraps air bubbles upon wetting the feature with a solvent or solution is described. The device includes a sacrificial residue in contact with the gas-entrapping feature, the dissolution of which guides the wetting of the gas-entrapping feature.

Abstract: One type of tissue-based assay, the companion diagnostic (“CDx”) allows for the identification of individuals within a larger patient population who are more likely to respond to a therapy. The CDx paradigm typically applies to drugs that target a specific gene product or biologic pathway involving a gene product of interest. It is possible, especially for popular therapeutic targets, for multiple drugs and multiple associated CDx to be developed for a single gene product or biologic pathway involving the gene product. Currently, each of these similar CDx must be applied to identify the best therapy. The present invention can determine the outcome of one CDx using an image of a tissue section used for another CDx. Using a single tissue section and a single CDx, it becomes possible to obtain the outcome of multiple, related CDx.

Abstract: Methods, systems, and devices for sampling/isolating material from cells. An exemplary system may comprise a chip including an electrode array of sampling electrodes arranged along a surface of the chip. A cell-receiving area may be located adjacent the surface of the chip. The system also may comprise a tag array of tags supported by the chip and aligned with the electrode array. Each tag of the tag array may include an identifier that is unique to the tag within the tag array. Each tag may be configured to bind nucleic acids, or a capturing agent distinct from the tag may be aligned with each sampling electrode of the electrode array to capture a protein or other analyte of interest. The system further may comprise a control circuit configured to apply an individually controllable voltage to each sampling electrode of the electrode array and measure an electrical property of the sampling electrode.

Abstract: A system and method for rapid detection of viable microorganisms (e.g., pathogens) in liquid media suspensions utilizes at least two electrodes in electrical communication with a suspension (e.g., liquid media possibly containing microorganisms). Electrical response to an electrical pulse in a short initial time window (e.g., no longer than a time required to attain 95% (or another threshold percentage) of a steady state electrical response value after a change in state of the pulse) permits bulk electrical response of the suspension between the electrodes to be determined before electrical response signals are dominated by double layer formation at surfaces of the electrodes. Pulse application and detection of electrical response to a change in state of a pulse may be repeated over time, with changes in such response being useful to detect microorganism proliferation in a bulk suspension.

Abstract: Described herein are methods, systems, and non-transitory computer-readable media to non-destructively acquire three-dimensional profiles of cellular microbiological samples growing on the surface of a solid growth medium. Acquisitions can be performed by an optical microscope that includes a vertical scanning interferometer. The three-dimensional profiles can enable measurement of sample parameters of microcolonies, which can be made of microbial colony forming units. The methods and systems enable early and rapid detection and quantification of microbes.

Abstract: Process for producing biomethane from a biogas stream including methane, carbon dioxide and at least one impurity chosen from ammonia, volatile organic compounds, water, sulfur-based impurities (H2S) and siloxanes. A biogas stream is dried, the at least one impurity is at least partially removed by solidification and removal of the impurity. The methane and the carbon dioxide contained in the biogas obtained from the second step are separated so as to produce a biomethane stream and a CO2 stream.

Abstract: Provided are compositions and methods for differentiating and diagnosing ischemic stroke and subgroups thereof (e.g., cardioembolic stroke, large vessel stroke, atherothrombotic stroke, lacunar stroke) from intracerebral hemorrhage.

Abstract: Method and apparatus for optimizing analyte sensor calibration including receiving a current blood glucose measurement, retrieving a time information for an upcoming scheduled calibration event for calibrating an analyte sensor, determining temporal proximity between the current blood glucose measurement and the retrieved time information for the upcoming calibration event, initiating a calibration routine to calibrate the analyte sensor when the determined temporal proximity is within a predetermined time period, and overriding the upcoming scheduled calibration event using the current blood glucose measurement are provided.

Abstract: A method for determining a value indicative of the permittivity of a cell population in the context of impedance spectroscopy comprises the following steps: generating an excitation current through the cell population, which oscillates with an excitation frequency; measuring a voltage in the cell population between a first measuring electrode (12) and a second measuring electrode (14); sampling the excitation current, wherein first sampled values for the excitation current are generated; sampling the voltage between the first measuring electrode (12) and the second measuring electrode (14), wherein second sampled values for the voltage between the first measuring electrode and the second measuring electrode are generated; and determining the value indicative of the permittivity of the cell population on the basis of the first sampled values and the second sampled values.

Abstract: The purpose of the present invention is to provide a method for accurately measuring and controlling intracellular potential by a simple method that is less invasive to the cell and does not require a skilled technique. The present invention makes it possible to provide an intracellular recording electrode inside the cytoplasm by introducing conductive nanoparticles into a cell cultured on a conductive plate electrode, attracting the conductive nanoparticles inside the cell to the side of the cell adhered to the conductive plate electrode, and causing the conductive nanoparticles to pass through the cell membrane. Measuring the current or voltage between the intracellular recording electrode and an extracellular electrode in extracellular solution makes it possible to measure the intracellular potential.

Abstract: A device and method for detecting particles by using electrical impedance measurement, in particular, relating to an improved electrical impedance measurement microfluidic chip and an improved particle detection method. The device comprises a sample injection part, a main channel (4) and an electrical impedance detection part. By means of said device and method, the present invention can accurately distinguish, detect and count different particles.

Abstract: Method and apparatus for optimizing analyte sensor calibration including receiving a current blood glucose measurement, retrieving a time information for an upcoming scheduled calibration event for calibrating an analyte sensor, determining temporal proximity between the current blood glucose measurement and the retrieved time information for the upcoming calibration event, initiating a calibration routine to calibrate the analyte sensor when the determined temporal proximity is within a predetermined time period, and overriding the upcoming scheduled calibration event using the current blood glucose measurement are provided.

Abstract: The invention relates generally to the THAP1 gene and mutations in this gene, as well as the THAP1 protein and mutations in this protein, that are associated with dystonia. The invention relates to the identification, isolation, cloning and characterization of the DNA sequence corresponding to the wild type and mutant THAP1 genes, as well as isolation and characterization of their transcripts and gene products. The invention further relates to methods and kits useful for detecting mutations in THAP1 that are associated with dystonia, as well as to methods and kits useful for diagnosing dystonia. The present invention also relates to therapies for treating dystonia, including gene therapeutics and protein/antibody based therapeutics.

Abstract: Biosensors utilizing 4-acetoxyphenol are described. The biosensors typically include 4-acetoxyphenol in a substrate and utilize one or more enzymes to detect the presence of pollutant agents. Also described are related methods using the biosensors to detect the presence of pollutant agents in water such as As(III).

Abstract: The present invention provides apparatus and methods for the rapid determination of analytes in liquid samples by immunoassays incorporating magnetic capture of beads on a sensor capable of being used in the point-of-care diagnostic field.

Abstract: Methods of measuring attributes of animal urine, using a test pad comprising multiple test patches and urine detection patches in a BAYER pattern is described. The pad comprises different test patches, each surrounded by urine detection patches. When a camera, electronics and software automatically detect fresh urine from a color change of a detection patch, nearby test patches are read with a color camera, after a specific time delay, and compared to color reference spots. Multiple layers and isolation zones in the test pad allow urine to enter the test and detection patches, while keeping urine puddles from spreading. Once used, detection and test patches are not used again. An array of many detection and test patches allows the test pad to be used for multiple urine samples in one vivarium cage before replacing. Embodiments use a mix of IR and white light, and IR cameras and color cameras.

Abstract: The present invention relates to analytical testing devices comprising fluidic junctions and methods for assaying coagulation in a fluid sample received within the fluidic junctions. For example, the present invention may be directed to a sample analysis cartridge including an inlet chamber, a first conduit comprising a first junction configured to split a biological sample into at least first and second segments, a second conduit comprising a first reagent, a first sensor region, and a first fluidic lock valve, and a third conduit comprising a second reagent, a second sensor region, and a second fluidic lock valve. The sample analysis cartridge further includes a pump configured to push the first segment over the first sensor region to the first fluidic lock valve, and push the second segment over the second sensor region to the second fluidic lock valve.

Abstract: The present invention provides a membrane carrier 3 comprising a flow path 2 and a detection zone 3y, wherein a microstructure is provided at the bottom of the flow path 2 and a mean surface roughness of the microstructure is 0.005 to 10.0 ?m.

Abstract: A microfluidic device includes a first substrate including at least one microfluidic channel and a plurality of microwells, as well as a cooperating second substrate defining multiple split-walled cell trap structures that are registered with and disposed within the plurality of microwells. A method for performing an assay includes flowing cells and a first aqueous medium into a plurality of microwells of a microfluidic device, wherein each microwell includes a cell trap structure configured to trap at least one cell. The method further comprises flowing a nonpolar fluid with low permeability for analytes of interest through a microfluidic channel to flush a portion of the first aqueous medium from the microfluidic channel while retaining another portion of the first aqueous medium and at least one cell within each microwell. Surface tension at a non-polar/polar medium interface prevents molecule exchange between interior and exterior portions of microwells.

Abstract: A first amount of sperm of a first animal and a second amount of sperm of a second animal of the same species, the first amount of sperm and the second amount of sperm sex-selected sperm of the same sex, useful in the in-vivo or in-vitro fertilization of an egg of a female animal of the same species of animal for the production of sex-selected embryos and sex-selected offspring.

Abstract: The present invention provides a membrane carrier 3 comprising a flow path 2 and a detection zone 3y, wherein a microstructure is provided at the bottom of the flow path 2 and a mean surface roughness of the microstructure is 0.005 to 10.0 ?m.

Abstract: A carrier plate, which is used within an electrophoresis process, and to a holding device tailored to the carrier plate, the carrier plate having a region with a magnetic property and a positioning device. The magnetic property is designed to fix the carrier plate in the laboratory device. The positioning device is designed to guarantee a position of the carrier plate in the laboratory device.

Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.

Abstract: A transport device transports a container used in a sample measurement device or a rack housing the container. The transport device may include: a robotic arm configured to transport the container or the rack between a first sample measurement device and each of other sample measurement devices different from the first sample measurement device; and a controller that controls operation of the robotic arm.

Abstract: One example includes a device that may include a heating element and a molecular binding site. The heating element may heat a fluid volume, interfaced with the heating element, in response to a voltage being applied to the heating element, the heat transforming the fluid volume from a liquid state into a vaporized state to generate fluid motion within the fluid volume. The molecular binding site may be disposed proximate to the heating element, in which a portion of the fluid volume expands when the fluid volume transforms from the liquid state into the vaporized state, the vaporized state of the fluid volume generating the fluid motion within a target fluid that is disposed within the molecular binding site.

Abstract: Provided is a material-fixing substrate that does not have to use copper as a catalyst because the substrate-bonding site includes a cyclic alkyne to form a covalent bond with a surface of the substrate, and therefore that can reduce damage to a cell, for example, in a case where a to-be-fixed material is the cell. The material-fixing substrate has a to-be-fixed material fixed thereon via a material-fixing agent. The material-fixing agent includes: a substrate-bonding site that forms a covalent bond with a surface of the substrate and includes at least a cyclic alkyne; a hydrophilic site that is bonded to the substrate-bonding site; a light-responsive site that is bonded to the hydrophilic site and changes the skeleton thereof by irradiation with light; and an attachment site to which the to-be-fixed material is attached.

Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.

Abstract: A method for metering and storing liquids by means of a permanently open container (1), wherein: the container (1) comprises a liquid reservoir (11) in which at least one liquid (2) is contained for storage; the container (1) has at least one opening (15) for the metered discharging of the liquid from the liquid reservoir (11); the opening (15) is permanently open; influencing factors affect at least one change in pressure (4) in the container (1); influencing factors render the system formed of the container (1) and liquid (2) unstable; the pressure (4) is detected in at least one location in the container (1); and, subject to at least one detected change in pressure (4), at least one reaction (9) is initiated that is suitable for maintaining the system of the container (1) and liquid (2) in a stable state or for re-establishing this stable state.

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

Abstract: A pipette based on surface charges includes a pipette body. A plurality of support rods are provided below the pipette body. Connecting rods are provided at the lower ends of two adjacent support rods. A substrate is provided between the connecting rods. A porous SiO2 coating is provided on the substrate. A hydrophobic molecule layer is provided on the surface coating by the vapor deposition method. A superamphiphobic surface is formed on the substrate by the surface coating and the hydrophobic molecule layer. The substrate and the surface coating are made of dielectric materials. A circular limit plate is provided above the pipette body. The pipette body, the support rods, the connecting rods and the limit plate are integrally formed, and are electrically non-conductive. An electrically conductive sliding rod is movably provided within the pipette body and the limit plate.

Abstract: Biologically low invasive vessels are filled with biological factors that have the activity of mobilizing specific functional cells in the body. The vessels are indwelled in the body. After specific functional cells are mobilized into the vessels, the vessels are removed from the body to collect functional cell populations mobilized to the vessels. Alternatively, the cells are directly collected from the vessels indwelled in the body.

Abstract: Provided is a cell culture apparatus including a cell supply unit that supplies cells; a culture medium supply unit that supplies a culture medium; an additive supply unit that supplies an additive for inducing the differentiation of undifferentiated cells; a stirring unit that stirs a processing target; a separation unit that separates a component contained in the processing target; a culture vessel that cultures the cells; a first flow channel that forms a circulation route passing through the cell supply unit, the stirring unit, the separation unit, and the culture vessel; a second flow channel that connects the culture medium supply unit and the first flow channel; a third flow channel that connects the additive supply unit and the first flow channel; and a control unit that controls the feeding of liquid through the first flow channel, the second flow channel, and the third flow channel.

Abstract: A sample preparation module accepts a sample including a target analyte. The sample preparation module processes the sample through several reaction chambers and a solid phase column. Different reagents are present in the reaction chambers. The eluted analyte is then transferred to the amplification module, where it is further processed and amplified for optical analysis.

Abstract: A method includes identifying a first user device potentially associated with a biohazard and identifying a geographic area associated with the biohazard based on previous location information of the first user device. The method further includes identifying a second user device potentially associated with the biohazard based on the geographic area associated with the biohazard and previous location information of the second user device. The method further includes issuing a safety notification to the second user device, where the safety notification includes one or more of the geographic area associated with the biohazard, a safety status request, a safety status level of the first user device, a subset of the previous location information of the first user device, and a subset of the previous location information of the second user device.

Abstract: A composition and a method for clearing tissue for subsequent three-dimensional analysis. The clearing composition comprises: (1) a homogenizing agent such as N-methylglucamine, urea, or ethylenediamine; (2) a cytoplasmic, water-soluble refractive index adjusting agent such as iohexol, sodium thiosulfate, or polyethylene glycol; and (3) a membrane, lipid-soluble RI adjusting agent such as 2,2?-thiodiethanol (TDE) or propylene glycol. The tissue clearing composition is particularly suitable for use with long-term fixed human tissues.

Abstract: The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

Abstract: A biological sample analysis device that is an easily portable free-standing device for determining the rate of deterioration of biological samples.

Abstract: The present invention is related to an electrochemical reactor (1) and a microfluidic platform (20) comprising this reactor (1), controlling pH in a closed environment, wherein this reactor (1) comprises at least one cell (2), wherein each cell (2) containing at least one micro-well (3a) able to contain a liquid and reagents and a cap (7) to close the said cell (2) and wherein the cell (2) further comprises at least one working electrode (5) producing reversible REDOX reactions.

Abstract: The present invention provides methods, devices, and compositions to rapidly detect analytes, including small analytes, using a lateral flow device. Described herein is such a lateral flow device that can detect and quantify vitamin D in a whole blood, serum, or plasma sample by employing a sandwich-based immunoassay.