Abstract: According to an embodiment of the present disclosure, provided is an apparatus for providing microorganism information, including: a receiving unit configured to receive a plurality of images obtained by photographing in time series an outgoing wave emitted from a sample; a detecting unit configured to extract a feature of a change over time from the plurality of images obtained by photographing in time series; a learning unit configured to machine-learn classification criteria based on the extracted feature; and a determining unit configured to classify the type or concentration of a microorganism included in the sample based on the classification criteria, wherein each of the plurality of images includes speckle information generated by multiple scattering by the microorganism due to waves incident on the sample.

Abstract: This application relates to a method for processing image data of a microbial culture medium to recognize colony forming unit (CFU). In one aspect, the method includes receiving, at a processor, captured image data of the microbial culture medium from a user device, and preprocessing, at the processor, the captured image data. The method may also include counting, at the processor, the number of CFUs included in the preprocessed image data to derive result data including the counted number of CFUs. The method may further include automatically inputting information included in the result data into a predetermined template to generate document data corresponding to the captured image data, and transmitting at least one of the result data or the document data to the user device.

Abstract: The present disclose provides methods and systems for amplifying and quantifying nucleic acids and for detecting the presence or absence of a target in a sample. The methods and systems provided herein may utilize a device comprising a plurality of partitions separated from an external environment by a gas-permeable barrier. Certain methods disclosed herein involve subjecting nucleic acid molecules in the plurality of partitions to conditions sufficient to conduct nucleic acid amplification reactions. The nucleic acid molecules may be subjected to controlled heating in the plurality of partitions to generate data indicative of a melting point(s) of the nucleic acid molecules.

Abstract: A system and process may be used to test water samples to measure ATP and/or estimate a microbial population, for example using an adenosine triphosphate (ATP) based assay. The system includes a device that is use in combination with single-use or disposable cartridges. The cartridge receives the water sample and is pre-loaded with one or more reagents. The device receives the cartridge and contains physical, electronic and/or mechatronic devices that interact with cartridge. One or more actions such as metering, mixing and conveying are performed automatically by elements of the device and/or cartridge. A sensor in the device measures light produced in the cartridge from a reaction with ATP in the water sample. Optionally, the cartridge also contains a pre-loaded amount of ATP, which is used to provide an internal reference or calibration measurement.

Abstract: An apparatus includes a bottom panel with a transparent region and ceramic sidewalls affixed to the bottom panel to form a container. Electrodes are disposed on the outer surface of the sidewalls at positions selected so that when a sample is positioned in the container, applying a voltage between the electrodes induces an electric field through the sample. Electrical conductors provide contact with the electrodes. All the components are sized and shaped to facilitate positioning of the container on the stage of an inverted microscope so that when the sample is positioned in the container, light emanating from a light source is free to travel along an optical path that passes through the sample, through the transparent region, and into the objective of the inverted microscope. The electrodes and conductors are positioned with respect to the transparent region so as not to interfere with the optical path.

Abstract: A variation of a method for estimating a quantity of a blood component in a fluid canister includes: within an image of a canister, identifying a reference marker on the canister; selecting an area of the image based on the reference marker; correlating a portion of the selected area with a fluid level within the canister; estimating a volume of fluid within the canister based on the fluid level; extracting a feature from the selected area; correlating the extracted featured with a concentration of a blood component within the canister; and estimating a quantity of the blood component within the canister based on the estimated volume and the concentration of the blood component within the canister.

Abstract: An incubator (1) and its use for cultivating microorganisms and/or for carrying out a chemical reaction are provided. The incubator (1) has a sample vessel holder (3) for insertion of a sample vessel (4), a heating device (5) for changing a temperature in the sample vessel holder (3) and/or in a sample vessel (4) inserted into the sample vessel holder (3), and a sample-inactivating device (6) for inactivation of a sample (7) in a sample vessel (4) that is inserted into the sample vessel holder (3).

Abstract: The disclosed systems and method utilize the autofluorescence, optic imaging, and heat transfer resistance technologies to monitor the same simulated surface area for deposits. The systems and methods may provide continuous monitoring, detection, characterization and quantification of deposits. Utilizing this information, an associated control system may initiate alarms, initiate a chemical treatment operation, and adjust corresponding chemical treatment and preventive protocols to minimize and/or eradicate the issue.

Abstract: An electronic package assembly includes a glass substrate including an upper glass cladding layer, a lower glass cladding layer, a glass core layer coupled to the upper glass cladding layer and the lower glass cladding layer, where the upper glass cladding layer and the lower glass cladding layer have a higher etch rate in an etchant than the glass core layer, a first cavity positioned within one of the upper glass cladding layer or the lower glass cladding layer, and a second cavity positioned within one of the upper glass cladding layer or the lower glass cladding layer, a microprocessor positioned within the first cavity, and a micro-electronic component positioned within the second cavity.

Abstract: Disclosed herein are systems and methods for imaging cells. Quantitative phase imaging uses variations in the index of refraction of a sample as a source of endogenous contrast, providing label-free information of sub-cellular structures and allowing for the reconstruction of valuable biophysical parameters, such as cell dry-mass at femtogram scales, mass transport, and sample thickness and fluctuations at nanometer scales. As a result, QPI has become a valuable tool in biology and medicine. However, QPI has suffered from the need for trans-illumination through relatively thin objects in order to gain access to the forward-scattered field, which carries crucial low spatial frequency information of a sample and avoid contributions from multiple scattered light or out-of-focus planes. The disclosed methods and systems can provide for reconstruction of QPI and corresponding analysis for imaging samples of cells in thick samples using an epi-illumination configuration.

Abstract: The present invention relates to a bioreactor including at least one reactor vessel in form of a plastic bag, a tray (4) for holding said at least one bag, a rocking device for limited rocking motions of the tray (4) around a rocking axis (2). According to the invention the bioreactor comprises a device (6,7,8,9) for enabling swinging of the tray (4) around a second axis (7) parallel to and distanced from the rocking axis (2).

Abstract: A system and method for analyzing bodily fluid include a sample holder holding a bodily fluid sample, an image capture device generating an image of the bodily fluid sample comprising a plurality of fields of view. An image processor is programmed to determine a biofilm in the bodily fluid sample from the image, determine a biofilm area or volume within each of the plurality of fields of view to form a plurality of biofilm areas, determine a total biofilm area or total biofilm volume by adding the plurality of biofilm areas, determine a first value corresponding to a comparison of the total biofilm area or the total biofilm volume and a total volume of the bodily fluid sample, and classify the first value into a classification. An analyzer, using the classification, displays an indicator on a display for indicating the classification of the biofilm within the bodily fluid sample.

Abstract: An apparatus and associated a method are described for performing gas analysis on a gas sample. The method comprising exciting the gas sample with one or more electromagnetic energy sources and obtaining optical absorption signals associated with the gas sample prior to application of a catalytic process to the gas sample as well as during and/or after application of the catalytic process to the gas sample. The obtained optical absorption signals may then be processed using differential calculation approaches to derive information associated with the gas sample, which may include for example information conveying concentrations of certain specific gases in the gas sample. In some implementations, the optical absorption measurement system is configured to use the one or more electromagnetic energy sources to excite the gas sample to produce first optical absorption signals.

Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analogue of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.

Abstract: In one embodiment, a fluorescence histo-tomography (FHT) system is disclosed. The FHT system includes an imaging housing, a fluorescence camera located within the housing, a white light camera located within the imaging housing, and a fluorescence light source located within the imaging housing. The FHT system further includes a support mount configured to support the imaging housing within a chamber of a slicing apparatus such that the cameras and fluorescence light source are aimed towards a block face of a tissue specimen retained within the chamber.

Abstract: A device is provided for capturing a nucleic acid in a single cell, having: a two-dimensional array comprising a substrate, a plurality of single cell capturing holes provided on one surface of the substrate, and a nucleic acid capturing region comprising, on the inside of the substrate, a nucleic acid capturing body, which is configured to capture the nucleic acids extracted from the individual cells respectively captured by the single cell capturing hole; a flow channel which is provided adjacent to the nucleic acid capturing region of the substrate, and is configured to discharge a solution in the nucleic acid capturing region; and a cylindrical structure body which is arranged on the substrate at the time of introducing a cell suspension and encloses a plurality of the single cell capturing holes, wherein the cylindrical structure body is removed after the cells are captured by the single cell capturing holes.

Abstract: The application provides heat-treated Limulus amebocyte lysates useful for detecting ?-glucans.

Abstract: An apparatus comprising a spectral filter (26) with a variable spectral filter transmission (FT(?)); an event-based imaging sensor (27) configured to produce measurement events (y1, y2, . . . , yn) which correspond to a change in a filter response (Y(t)) that is generated by an observed spectrum (X(?)), and a processor (28) configured to control the filter transmission (FT(?)) of the spectral filter (26) so that it sweeps over wavelength (?) with time (t), and to generate an estimation (X*(?)) of the observed spectrum (X(?)) based on the measurement events (y1, y2, . . . , yn) that correspond to the filter response (Y(t)) and based on the filter transmission (FT(?)).

Abstract: Methods and apparatus for detecting, quantifying, enriching, and/or separating bacterial species in fluid sample are provided. The fluid sample is provided as input to a microfluidic passage of a microfluidic device, wherein the microfluidic device comprises at least one electrode disposed adjacent to the microfluidic passage. The at least one electrode is activated to capture bacteria in the sample using dielectrophoresis, wherein the capture efficiency of bacteria is at least 99%.

Abstract: Techniques regarding integrated purification-detection devices for detecting one or more biomarkers are provided. For example, one or more embodiments described herein are directed to an apparatus, comprising a housing and a microfluidic chip contained within the housing. The microfluidic chip comprises a separation unit that separates, using one or more nano deterministic lateral displacement (nanoDLD) arrays, target biological entities having a defined size range from other biological entities included in a biological fluid sample. The microfluidic chip further comprises a detection unit that facilitates detecting presence of one or more biomarkers associated with the target biological entities using one or more detection molecules or macromolecules that chemically reacts with the one or more biomarkers.

Abstract: A manufacturing facility for the continuous production of biopharmaceuticals integrated with single-use disposable technology.

Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analogue of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.

Abstract: A bioreactor outlet air conditioning system (10) has a bioreactor vessel (100) and a heat exchanger. The heat exchanger has a fluid flow path (196) from a headspace (108) in the vessel for venting air from the vessel, and a temperature control element (300) in thermal contact with the fluid flow path (196). The fluid flow path (196) may be defined by a disposable portion of the bioreactor vessel (100). A method of controlling evaporation within a bioreactor vessel (100), dependent on the required evaporation rate, adjusts the temperature of the temperature control element (300) to control the rate of evaporation of the liquid media (106) from the vessel (100), and may include monitoring fluids in the fluid flow path (196) to detect at least water content of the fluids exiting the vessel to adjust the temperature and control the rate of evaporation dependent on the detected water content levels.

Abstract: Modules, devices, systems and methods for measuring or detecting cysteine and/or methionine metabolite levels in a sample from a subject are disclosed. Various embodiments of the present invention concern modules, devices, systems and methods for prognosing or diagnosing cancer, for example, prostate, colon, ovarian or breast cancer; predicting the risk or probability of cancer recurrence; and/or for predicting, detecting and/or monitoring cystinuria or cystine stone disease.

Abstract: A cartridge and a method for testing a biological sample are provided, wherein the cartridge is filled, in an open packaging, with the sample to be tested, and wherein the packaging holds and/or supports the cartridge in a latching manner in an open state.

Abstract: The present invention provides self-contained systems for performing an assay for determining a chemical state, the system including a stationary cartridge for performing the assay therein, at least one reagent adapted to react with a sample; and at least one reporter functionality adapted to report a reaction of the at least one reagent with said sample to report a result of the assay, wherein the at least one reagent, the sample and the at least one reporter functionality are contained within the cartridge.

Abstract: A biological sample reaction vessel comprising a reagent storage portion and a push rod movable relative to the reagent storage portion is provided. The reagent storage portion comprises at least one reagent containing cavity, and the reagent containing cavity is sealed by a sealing element; and the push rod is connected to the sealing element, and the push rod is used for cooperation with an external test device to separate the sealing element from the reagent storage portion. In reaction, the biological sample reaction vessel cooperates with a test cassette. By inserting the biological sample reaction vessel into the external device, the reagent in the reagent storage portion can be released rapidly.

Abstract: Methods and apparatuses for tracking and correlating particles include an optical detector that captures a first and a second image of the particles. A video detector is used to capture a plurality of video frames of the particles. The video detector captures the video frames of the particles at a rate faster than the rate at which images are captured by the optical detector to track the movement of particles. A first image position of a particle in the first image of the particles is identified, and then the first image position of the particle is correlated to a second image position of the particle in the second image using the plurality of video frames.

Abstract: An example system for inspecting a surface includes a laser, an optical system, a gated camera, and a control system. The laser is configured to emit pulses of light, with respective wavelengths of the pulses of light varying over time. The optical system includes at least one optical element, and is configured to direct light emitted by the laser to points along a scan line one point at a time. The gated camera is configured to record a fluorescent response of the surface from light having each wavelength of a plurality of wavelengths at each point along the scan line. The control system is configured to control the gated camera such that an aperture of the gated camera is open during fluorescence of the surface but closed during exposure of the surface to light emitted by the laser.

Abstract: A hand-held microfluidic testing device is provided that includes a housing having a cartridge receiving port and a cartridge for input to the cartridge receiving port. An optical detection system in the housing is capable of providing an illuminated electric field useful for Raman spectroscopy. The cartridge may have a sample well. The sample well is loaded with a mixture of water containing the analyte, Raman-scattering nanoparticles and a calibration solution. The calibration solution contains an analogue of the analyte differing in its Raman response, for example an isotope of the analyte. Optionally, a chemical compound capable of increasing interaction between the analyte and the nanoparticles may be added.

Abstract: The present disclosure relates to a method for high precision laser processing of sapphire with submicron-order section plane using a picosecond-order pulse laser which has high transmittance wavelength to sapphire. The laser triggers ultrafine phase transformation points or electronic state removal points from a lower surface of sapphire. After elevating focal points, a trace which is parallel to laser incident direction is formed. Under a chemical corrosion environment, points of the laser trace are arranged to intersect with other another according to the cutting route to form the corresponding phase transformation region and electronic state removal region. At the same time, by utilizing the catalysis effect of microthermal effect of picosecond laser on chemical corrosion, separation of the sapphire sample along the processing path is obtained.

Abstract: Apparatus and methods for using a flow cell array are provided herein. A method includes delivering multiple items of chemical matter independently to multiple reaction sites of a flow cell array across multiple distinct instances of time; imaging multiple parallel chemical reactions at the multiple reaction sites of the flow cell array; and recording an emission from each of the multiple chemical reactions site.

Abstract: A cell-spreading device may include a microchamber chip having a microchamber capable of enclosing and retaining a cell, a channel-forming frame united with the microchamber chip to form a channel on the microchamber, an inlet provided in the channel-forming frame to allow a cell suspension to flow into the channel, and an outlet provided in the channel-forming frame to allow the cell suspension, which has been allowed to flow into the channel through the inlet, to flow out from the channel. When an aperture of the microchamber is projected perpendicularly to a longitudinal width of the microchamber chip, the void ratio that is a ratio of the sum total of voids to the longitudinal width is not more than 5%, the void being a length of a portion where the projected aperture of the microchamber is not present against the longitudinal width.

Abstract: Disclosed are methods, systems, and apparatuses for non-invasive detection of colitis in a subject. The methods involve depositing a bodily fluid sample from the subject on an internal reflection element (IRE). A beam of infrared (IR) radiation can then be directed through the IRE under conditions such that the IR radiation interacts with the bodily fluid sample. An absorption spectrum can then be recorded over a range of preselected frequencies to detect peaks that are affected by colitis. In preferred embodiments, the methods and systems involve Fourier Transform Infrared Spectroscopy (FTIR).

Abstract: A single-use, consumable diagnostic cartridge and method of performing a diagnostic test are provided. The cartridge has a sample inlet opening, an inlet port, a sample receiving chamber, an analysis chamber and a fluid channel extending between the sample receiving chamber and analysis chamber. A rupturable blister contains a fluid for selective mixing with a sample selectively disposed in the sample receiving chamber. A valve member, having a sample through port and a fluid passage, is selectively moveable between closed and open states. In the closed state, the fluid passage is out of fluid communication with the sample, and the sample through port is registered with the sample inlet opening and the sample receiving chamber. In the open state, the fluid passage extends between the inlet port and the sample receiving chamber to allow fluid dispelled from the blister to transport the sample to the analysis chamber.

Abstract: The invention relates to a method for quantitative measurement of a biomarker by in situ immunofluorescence and uses thereof. In particular, the invention relates to a method which is a useful tool for use in the field of diagnosis, prevention and/or treatment of disease or disorders, in particular in the field of cancer management and therapy.

Abstract: Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Abstract: Provided are nanograting structures and methods of fabrication thereof that allow for stable, robust gratings and nanostructure embedded gratings that enhance electromagnetic field, fluorescence, and photothermal coupling through surface plasmon or, photonic resonance. The gratings produced exhibit long term stability of the grating structure and improved shelf life without degradation of the properties such as fluorescence enhancement. Embodiments of the invention build nanograting structures layer-by-layer to optimize structural and optical properties and to enhance durability.

Abstract: A cell culture apparatus including cell culture units U and a measurement part (5). The measurement part (5) is provided with an imaging part (51) and is disposed so as to be capable of moving up and down between a back panel part (21) of a casing (2) and a culture container accommodating shelf (32). When the measurement unit (5) reaches a measurement position of each cell culture unit U which is a measurement subject, the culture container accommodating shelf (32) slides toward the measurement unit (5) so that portions to be measured of culture containers U20, U30 in each cell culture unit U come in an imaging range of the imaging part (51).

Abstract: A diagnostic cartridge and method of performing a diagnostic test are provided. The cartridge includes a body having a sample chamber for receipt of a sample, an analysis chamber, and a reagent-containing dispensing member. A valve member is coupled to the body for selective movement between first and second states. The valve member has a fluid passage with a hydroscopic, gas permeable vent. In the first state, the fluid passage is out of fluid communication with the sample chamber and is registered for fluid communication with the reagent-containing dispensing member. The vent prevents fluid from passing therethrough and allows air to vent therefrom as reagent flows into and fills the fluid passage. In the second state, the fluid passage remains in fluid communication with the reagent-containing dispensing member and is brought into fluid communication with the sample chamber to facilitate transporting the sample to the analysis chamber.

Abstract: A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

Abstract: Described here is a microfabricated particle sorting device that uses a transient pulse of fluidic pressure to deflect the target particle. The transient pulse may be generated by a microfabricated (MEMS) actuator, which pushes a volume of fluid into a channel, or sucks a volume of fluid from the channel. The transient pressure pulse may divert a target particle into a sort channel.

Abstract: A detector arrangement is disclosed for a blood culture bottle incorporating a colorimetric sensor which is subject to change of color due to change in pH or CO2 of a sample medium within the blood culture bottle. The detector arrangement includes a sensor LED illuminating the colorimetric sensor, a reference LED illuminating the colorimetric sensor, a control circuit for selectively and alternately activating the sensor LED and the reference LED, and a photodetector. The photodetector measures reflectance from the colorimetric sensor during the selective and alternating illumination of the colorimetric sensor with the sensor LED and the reference LED and generates intensity signals. The reference LED is selected to have a peak wavelength of illumination such that the intensity signals of the photodetector from illumination by the reference LED are not substantially affected by changes in the color of the colorimetric sensor.

Abstract: Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

Abstract: An optical distance measuring apparatus includes: a scanning element scanning a coherent irradiation light from a light source and sending it to an object under measurement; a photo detector receiving the irradiation light modulated by being passed through the object under measurement in accordance with the scanning, and performing photoelectric conversion on the irradiation light; and a measuring unit obtaining phase information of the object under measurement based on a signal photoelectrically converted by the photo detector and a signal to be a reference for the scanning by the scanning element, and obtaining a measurement value regarding the object under measurement based on the phase information.

Abstract: A cell picking support device, a display device and a culture container that can enhance work efficiency. An adapter fixes a well plate that holds cells cultured in a cell culture observation device. A control unit reads culture observation information that includes cell information and coordinate information, from a memory card that is fitted to an external memory I/F. A display unit is provided on a placing surface side of the well plate that is fixed by the adapter, and in response to control by the control unit, displays the cell information, read from the memory card, at display positions corresponding to positions on the bottom face of the well plate, as specified by the coordinate information. Embodiments can be used, for instance, as a cell picking support device that supports a picking operation that is performed in a clean bench.

Abstract: The present invention relates to devices and methods for measuring the quantity of multiple analytes in a sample. The device is designed such that each of the analyte sensing elements is configured to measure the quantity of a predetermined analyte and where the machine executable instructions are configured to select the proper analyte sensing element corresponding to the analyte to be measured.

Abstract: Disclosed is an analyte detection method for detecting an analyte contained in a biological sample, constituted by irradiating a light of a first peak wavelength on a complex containing an analyte and fluorescent substance which gives off light of a second peak wavelength of 450 nm or higher but not exceeding 900 nm when irradiated with light of the first wavelength of 190 nm or higher but not exceeding 350 nm, detecting the light of the second peak wavelength given off by the fluorescent substance when irradiated by light of the first peak wavelength, by a photodetector, wherein the photodetector has a quantum efficiency in the second peak wavelength that is two or more times the quantum efficiency in the first peak wavelength.

Abstract: A 3D printing device is provided. The 3D printing device is adapted to print a 3D object including a plurality of layer objects. The 3D printing device includes a weight sensor, which is configured to measure an actual weight value of the layer objects formed on a carrying surface or configured to measure an actual weight value of a feed tray. A controller of the 3D printing device estimates an ideal weight value corresponding to a first layer object according to a printing sequence of the first layer object in the layer objects. The controller receives the actual weight value corresponding to the first layer object from the weight sensor, and compares the ideal weight value with the actual weight value to determine whether printing abnormity is occurred. According to the disclosure, the printing quality of the 3D printing device is improved.

Abstract: An embodiment of a device for automatically executing a process of generating an emulsion containing nucleic acids, amplifying the nucleic acids in the emulsion, breaking the emulsion, and separating and purifying said amplified nucleic acids, is described that comprises an emulsion generation unit for sealing beads to which nucleic acids are bound in a water-in-oil type emulsion; a nucleic acid amplification unit provided with a reaction vessel for amplifying said nucleic acids and a heating and cooling part for heating and cooling the reaction vessel; an emulsion breaking unit for breaking the emulsion after nucleic acid amplification; and a nucleic acid purification unit for recovering said amplified nucleic acids from said emulsion breaking unit.