In Combination With A Dish, Plate, Or Tray Patents (Class 435/297.5)
  • Patent number: 10179928
    Abstract: In a first aspect, the present invention relates to a method for screening bacteria on their susceptibility against candidate compounds. In a further aspect, the present invention relates to a method for screening the antibiotic efficacy of candidate compounds suppose to have an antibiotic activity on bacteria. Moreover, the present invention relates to a method for forming a bacterial biofilm on a support, a system allowing in vitro and in vivo evaluation of biofilms formed by bacteria as well as methods for the stratification of the treatment regimen against bacterial infections.
    Type: Grant
    Filed: July 30, 2010
    Date of Patent: January 15, 2019
    Assignee: Helmholtz-Zentrum fuer Infektionsforschung GmbH
    Inventors: Mathias Müsken, Susanne Häußler
  • Patent number: 10053663
    Abstract: The invention is directed to a centrifuge chamber comprising a cylinder having a base plate and a cover plate, a rotational axis with at least one port for input and/or output of liquids, at least one port for input of gases and at least one layer for cell culturing, wherein the layer for cell culturing comprises a gas-permeable membrane on which cells can be cultured and wherein the at least one port for input and/or output of gases is connected to the gas-permeable membrane.
    Type: Grant
    Filed: May 23, 2016
    Date of Patent: August 21, 2018
    Assignee: Miltenyi Biotec, GmbH
    Inventors: Eiad Kabaha, Stefan Miltenyi, Ralf-Peter Peters
  • Patent number: 9976942
    Abstract: A cryogenic tissue sample embedding storage system belongs to the field of cryopreservation of biological tissue samples, which in particular, relates to a cryogenic tissue sample embedding storage system. The present invention provides a cryogenic tissue sample embedding storage system that can avoid the confusion of samples, prevent damage to identifier information in the cutting process, and has high embedding efficiency. The present invention comprises an embedded label, a label cover, and an embedding base, and in terms of its structure, the embedding base is provided with a groove structure for placing and limiting the position of the embedded label, and an embedding enclosure wall flexibly connected to the embedding base is disposed above the groove structure.
    Type: Grant
    Filed: February 15, 2017
    Date of Patent: May 22, 2018
    Assignee: THE FIRST HOSPITAL OF CHINA MEDICIAL UNIVERSITY
    Inventors: Ruiqun Qi, Xinghua Gao, Hongduo Chen
  • Patent number: 9434924
    Abstract: Provided is a means suitable for mass culture of adherent cells and which allows easy and safe collection of the cultured adherent cells. A cell culture kit has a culture bag having flexibility, such a level of hydrophilicity that the culture of adherent cells can be achieved, and permeability into a bag shape and which is filled with a culture medium; a dissociation solution bag filled with a dissociation solution containing a metal chelating agent or ornithine or a derivative thereof, and a solution sending circuit or a derivative thereof, and a solution sending circuit which connects the culture bag and the dissociation solution bag to each other and can send the dissociation solution from the dissociation solution bag to the culture bag.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: September 6, 2016
    Assignee: Nipro Corporation
    Inventors: Yoshihiro Yoshikawa, Naomi Morikawa, Ryo Tomii
  • Patent number: 9339818
    Abstract: A device for isolating and cultivating live cells on a filter or for extracting the genetic material thereof includes: a filter holder (108) connected to a filter; a compartment (102) having an upper opening and a lower opening; and an element (110) that is mobile relative to the compartment for applying a force on the holder and releasing the holder. According to the embodiments, the filter holder is mechanically connected to the compartment or to the mobile element until the application of the force. Preferably, the device further includes a removable end piece (104) tightly and removably attached and adapted for preventing the relative movement of the mobile element and the compartment for applying the force and releasing the holder.
    Type: Grant
    Filed: January 8, 2009
    Date of Patent: May 17, 2016
    Assignee: SCREENCELL
    Inventor: Yvon Cayre
  • Patent number: 9200245
    Abstract: A mulitwell plate having a plurality of picowells on the bottom of the wells of the plate as well as methods of producing the mulitwell plate are provided. Provided is also a method of handling living cells by providing an ordered array of living cells immobilized in a non-fluid matrix, contacting the living cells with a stimulus; and detecting a response to the stimulus. The present invention is also of a method of producing an ordered array of living cells.
    Type: Grant
    Filed: July 20, 2004
    Date of Patent: December 1, 2015
    Assignee: Seng Enterprises Ltd.
    Inventors: Mordechai Deutsch, Max Herzberg, Reuven Tirosh, Assaf Deutsch
  • Patent number: 9200246
    Abstract: An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.
    Type: Grant
    Filed: December 1, 2011
    Date of Patent: December 1, 2015
    Assignee: ACEA Biosciences, Inc.
    Inventors: Donald Thomas, Wei Li, Ning Ke, Nan Li, Adrian Papas, Manfred Watzele, Alexander Seiler, Tianxing Wang, Yama Abassi, Xiaobo Wang
  • Patent number: 9176060
    Abstract: The present invention provides apparatus and methods for measuring the optical density of a fluid by measuring the light transmission through a reconfigurable optical path. The optical path is reconfigured by the deflection of an elastic membrane wherein the optical path length through the fluid is determined by the position of the membrane relative to a fixed surface. The optical density can be determined from two measurements with different optical path lengths through the fluid.
    Type: Grant
    Filed: April 9, 2012
    Date of Patent: November 3, 2015
    Assignee: Pharyx, Inc.
    Inventors: Harry Lee, Kevin Shao-Kwan Lee
  • Patent number: 9175254
    Abstract: Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of the culture vessel, top and bottom. A gas-permeable membrane that includes perfluorocarbons or fluorocarbon derivatives (e.g., fluorinated silane, partially fluorinated silane) in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which the cell culture apparatus is incubated.
    Type: Grant
    Filed: October 7, 2013
    Date of Patent: November 3, 2015
    Assignee: University of Miami
    Inventors: Christopher A. Fraker, Juan Dominguez-Bendala, Camillo Ricordi, Luca Inverardi
  • Patent number: 9139804
    Abstract: An insert for cell cultures has a hollow housing (1) of a cylinder-like shape, with an upper, in particular circular, opening (2) and a membrane-like base located opposite the opening (2). On the peripheral opening edge (6) outward protruding support arms (5) are spaced apart from one another. The housing (1) has on its outer peripheral surface (12), between at least two support arms (5), a flattened region (11) extending from the opening edge (6) in the direction of the base (3), so that in a plane perpendicular to the central longitudinal axis (Y) of the housing (1) in the region of the flattened region (11) there is a radial clearance of the peripheral surface (12) relative to the longitudinal axis (Y) which is smaller than the radial clearance and/or outer radius of the peripheral surface (12) in the non-flattened region of the peripheral surface (12).
    Type: Grant
    Filed: January 23, 2012
    Date of Patent: September 22, 2015
    Assignee: Sabeu Kunststoffwerk Northeim GmbH
    Inventor: Nikolas Dicke
  • Patent number: 9057047
    Abstract: A device for cell culture includes a container body, which has an internal compartment to contain a quantity of cells to be cultured and a culture surface for the cells placed in the internal compartment; an introduction opening for introducing the cells to be cultured in the internal compartment; the container body having a first half shell and a second half shell, which can be fixed in mutually facing relation by a removable fixing system and which together define the internal compartment and the culture surface; and at least one out feed opening for a transport and/or culture fluid for the cells.
    Type: Grant
    Filed: August 6, 2009
    Date of Patent: June 16, 2015
    Assignee: RIGENERAND SRL
    Inventors: Pierfranco Conte, Massimo Dominici, Gianni Bellini, Matteo Brogli
  • Publication number: 20150093818
    Abstract: Methods and apparatuses for generating microscopic patterns of macromolecules such as proteins on a solid surface are described. Pulsed laser light is used to alter surface portions of a solid surface substrate in a predetermined pattern by removing macromolecules from surface portions of the substrate where the light is focused. The same wavelength light at lower intensity can be used to visualize the removal by its reflection from the specimen surface along the path to the detector. Select macromolecules introduced to the substrate selectively adhere to select surface portions, thereby depositing macromolecules in the predetermined pattern on the solid surface.
    Type: Application
    Filed: November 21, 2014
    Publication date: April 2, 2015
    Inventors: Paul C. Bridgman, Stephen G. Turney
  • Patent number: 8980625
    Abstract: A cell culture plate has a plurality of flow channels, uneven pattern areas and through holes. The flow channels are formed between the uneven pattern areas, and culture solution flows from the flow channels into the uneven pattern areas or from the uneven pattern areas into the flow channels. The uneven pattern area has uneven patterns that create a cell culture space.
    Type: Grant
    Filed: February 24, 2006
    Date of Patent: March 17, 2015
    Assignees: National Food Research Institute, Kuraray Co., Ltd.
    Inventors: Yuji Kikuchi, Hiroko Kikuchi, Taiji Nishi, Takenori Kitani, Yukihiro Yanagawa, Motohiro Fukuda
  • Patent number: 8969073
    Abstract: The invention describes an appliance and a method, with the help of which specific bio-particles, but also dissolved bio-molecules can be recognized in and separated from fluids making use of suitable carriers and known immobilization methods. The appliance can be used both discontinuously and also for direct and continuous treatment of fluids. Fields of application of the invention are animals, bio-technology (including biological research) and medicinal diagnostics. Areas of application of the invention comprise, among others, therapy of humans, in particular direct treatment of blood.
    Type: Grant
    Filed: January 30, 2007
    Date of Patent: March 3, 2015
    Assignee: pluriSelect GmbH
    Inventor: Hans Werner Heinrich
  • Patent number: 8951788
    Abstract: The invention relates to a filtration unit, comprising a membrane filter that can be disposed on a filter support of a bottom part and an attachment that can be placed on the bottom part, wherein the membrane filter has a reinforcing edge, and wherein the membrane filter can be clamped to a clamping part of a cover of a culture medium unit for removal purposes and introduced into the culture media unit. The invention further relates to a method for the microbiological analysis of liquid samples following a filtration process, wherein after removing an attachment a membrane filter is lifted off a filter support for pouring in the liquid sample and set down on a surface of a culture medium disposed in the bottom part of a culture medium unit, and the bottom part is covered by a cover. The cover is placed on the membrane resting on the filter support such that a clamping part present on the filter support clamps to a reinforcing edge of the membrane.
    Type: Grant
    Filed: February 16, 2008
    Date of Patent: February 10, 2015
    Assignee: Sartorius Stedim Biotech GmbH
    Inventor: Karl Pflanz
  • Patent number: 8940528
    Abstract: A dish for cell cultivation and microscopy is provided, comprising a ring and an outer ridge upwardly and axially protruding therefrom, wherein the ring is covered at its bottom side with a transparent membrane and, thus, forms a recess delimited by the inner side of the ring and the membrane, characterized in that the recess is filled with an adhesive filling. Alternatively, a dish for cell cultivation and microscopy is provided, comprising a base and an outer ridge protruding upwardly and axially at its rim, wherein the base and the outer ridge are made of a transparent plastic, the base together with the inner side of the outer ridge forms a recess filled with an adhesive filling, and the thickness of the base and the filling together is less than about 1 mm.
    Type: Grant
    Filed: July 20, 2009
    Date of Patent: January 27, 2015
    Assignee: Molecular Machines & Industries AG
    Inventor: Stefan Niehren
  • Publication number: 20150024478
    Abstract: The invention relates to the use of single- or multilayer plastic web plates in a sloped channel-type solid material separator with a lamella package.
    Type: Application
    Filed: February 20, 2013
    Publication date: January 22, 2015
    Applicants: BAYER TECHNOLOGY SERVICES GMBh, MAYER INTELLECTUAL PROPERTY GMBH
    Inventors: Andre Pastor, Juri Seletzky, Helmut Brod, Joerg Kauling, Peter Commer
  • Patent number: 8927267
    Abstract: The present invention relates generally to a multi-layer system for containing cells in culture, and a system for visualizing cells cultured in the multi-layered system. More specifically, the present invention relates to a multi-layer cell culture device having a specialized bottom plate and a microscope adaptor which can accommodate a microscope to allow microscopic visualization of cells cultured in the device.
    Type: Grant
    Filed: September 19, 2011
    Date of Patent: January 6, 2015
    Assignee: Corning Incorporated
    Inventors: Scott Matthew Bennett, David Alan Kenney, Gregory Roger Martin, Jeffry J Santman, Allison Jean Tanner
  • Publication number: 20140349382
    Abstract: The present invention relates to an apparatus for, and methods of assessing cell viability in a biological sample comprising cells or tissue. In particular the present invention provides quality assurance assays for complex biomaterials, especially but not exclusively cells derived from the pancreas, liver, kidney, lung, bone marrow and stem cells, for use in biomedical procedures. The present invention provides inter alia methods of improving assessment of donor cell/tissue viability, methods of improving the success of donor cell/tissue transplant procedures, methods of assessing the functional properties of complex biological materials prior to their use in regenerative therapies and kits therefor.
    Type: Application
    Filed: December 21, 2012
    Publication date: November 27, 2014
    Applicant: University of Leeds
    Inventors: Brian Mark Thomson, Mark Frederick Smith
  • Patent number: 8852881
    Abstract: A device for characterizing the biological properties of cells can include a plurality of dual-compartment assay chambers wherein the compartments of each chamber are separated by a cell layer across which ions can flow. The biological properties of the cell layer in the presence or absence of experimental compounds can be determined by measuring an electrical gradient across the layer. A individual dual-compartment chamber of this type may be referred to as an “Ussing chamber.
    Type: Grant
    Filed: November 29, 2006
    Date of Patent: October 7, 2014
    Assignee: Vertex Pharmaceuticals, Inc.
    Inventors: Paul A. Negulescu, Alec T. Harootunian, Philip E. Salzmann, Javier H. Flores, James E. Sinclair, Minh Vuong, Ashvani K. Singh, Fred F. VanGoor
  • Patent number: 8808541
    Abstract: A dialysis cell is provided for the measurement of free thyroxine. The dialysis cell preferably includes a polyhedral housing including a top, a bottom and four sides. The dialysis cell is made up of a buffer portion and serum portion. The buffer portion includes a cavity and the serum portion includes a cavity which, when the buffer portion and serum portion are assembled together, form a central chamber. The central chamber is divided by a vertically aligned dialysis membrane held in place by two O-rings. Buffer is introduced into the dialysis cell's buffer portion through a buffer inlet extending from the dialysis cell's top side to the buffer portion's cavity. Similarly, serum is introduced into the serum portion through an inlet which extends from an opening formed on the dialysis cell's top to the serum portion's cavity.
    Type: Grant
    Filed: March 3, 2008
    Date of Patent: August 19, 2014
    Inventor: Marwan Nasralla
  • Patent number: 8809044
    Abstract: This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.
    Type: Grant
    Filed: December 7, 2007
    Date of Patent: August 19, 2014
    Assignee: Wilson Wolf Manufacturing Corporation
    Inventor: John R. Wilson
  • Patent number: 8802391
    Abstract: The present invention is directed to a method for measuring the migration of cells in a channel under the influence of an analyte wherein said cells are separated from said analyte by a semi-permeable membrane and said cells are subjected to controlled flow conditions while the analyte is static and in the form of a viscous substance or gel.
    Type: Grant
    Filed: October 10, 2012
    Date of Patent: August 12, 2014
    Assignee: Cellix Limited
    Inventors: Dmitry Kashanin, Igor Shvets, Vivienne Williams, Frank O'Dowd
  • Patent number: 8785181
    Abstract: Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided.
    Type: Grant
    Filed: October 16, 2012
    Date of Patent: July 22, 2014
    Assignee: Terumo BCT, Inc.
    Inventor: Glen Delbert Antwiler
  • Patent number: 8779779
    Abstract: Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.
    Type: Grant
    Filed: October 24, 2008
    Date of Patent: July 15, 2014
    Assignees: CapitalBio Corporation, Tsinghua University
    Inventors: Lei Wang, Jing Zhu, Cheng Deng, Jing Cheng
  • Publication number: 20140178992
    Abstract: A membrane separation culture device includes an upper structure including a vessel in which at least a portion of the bottom thereof is formed with a separation membrane having pores that allow stem cells to permeate therethrough, and a lower structure including a vessel that retains a fluid in which the membrane of the upper structure is immersed.
    Type: Application
    Filed: March 30, 2012
    Publication date: June 26, 2014
    Applicant: National Center for Geriatrics and Gerontology
    Inventors: Misako Nakashima, Koichiro Iohara, Kazumasa Yamada, Masaaki Shimagaki, Masahiro Osabe
  • Publication number: 20140134716
    Abstract: A tissue culture device includes a container having one or more upstanding walls extending upwardly from a floor. The floor of the container has a media having nutrients or growth substances therein. A plurality of plant tissues are within the container compartment and are placed upon a screen between the plant tissues and the media. The screen is tamped downwardly onto the media so that the plant tissues can get nutrients from the media, and so that waste products are transferred into the media. The screen is also removable through the open end of the container so as to remove all of the plurality of the plant tissues from the container at once. The plant tissue device can then be placed in another container having any cultured media as needed for the correct maintenance, propagation and development of plant tissue in culture.
    Type: Application
    Filed: January 21, 2014
    Publication date: May 15, 2014
    Inventors: Steven M. Becker, Sam Ellis, Yinghong Li, Igor C. Oliveira
  • Patent number: 8709796
    Abstract: The invention concerns a device for preparing a body fluid for a bacteriological analysis thereof comprising a container provided with a chamber (4) wherein a piston (5) is mobile between an opening position and a closing position, the chamber (4) including a separation zone (7) and means for introducing (15, 16) a fluid into said chamber and the piston (5) including a closure means (8) co-operating with the separation zone (7) so as to define an upper volume (9) and a lower volume (10) on either side of said zone, the upper volume (9) and the lower volume (10) mutually communicating when the piston (5) is in opening position and being tightly isolated from each other when the piston (5) is in closing position. The invention also concerns a preparation method using such a device (1).
    Type: Grant
    Filed: July 11, 2006
    Date of Patent: April 29, 2014
    Assignee: Becton, Dickinson and Company
    Inventors: Isabelle Besson Faure, Jean-Pierre Hermet, Sébastien Ribault
  • Patent number: 8697443
    Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.
    Type: Grant
    Filed: April 2, 2010
    Date of Patent: April 15, 2014
    Assignee: Wilson Wolf Manufacturing Corporation
    Inventors: John R. Wilson, Douglas A. Page, Daniel P. Welch, Alison Robeck
  • Publication number: 20140099717
    Abstract: Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of the culture vessel, top and bottom. A gas-permeable membrane that includes perfluorocarbons or fluorocarbon derivatives (e.g., fluorinated silane, partially fluorinated silane) in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which the cell culture apparatus is incubated.
    Type: Application
    Filed: October 7, 2013
    Publication date: April 10, 2014
    Inventors: Christopher A. Fraker, Juan Dominguez-Bendala, Camillo Ricordi, Luca Inverardi
  • Patent number: 8685345
    Abstract: An insert for a sample chamber of a multi-well plate, which delimits the sample chamber upwardly, so as to form a measurement space, when the insert is in the state in which it is fitted into the sample chamber. The insert has at least two portions with outer contours differing from one another, one of the portions being a sealing portion with a continuous sealing surface, which sealing portion is adapted to a corresponding, in particular hollow-cylindrical sealing portion, with a likewise continuous sealing surface, of the sample chamber, in such a way that the insert sealing portion and the sample chamber sealing portion can be connected to one another releasably and in a liquid-tight manner by means of a press fit.
    Type: Grant
    Filed: August 20, 2012
    Date of Patent: April 1, 2014
    Assignee: Erwin Quarder Systemtechnik GmbH
    Inventor: Eike Kottkamp
  • Patent number: 8597590
    Abstract: Systems and methods for multiple analyte detection include a system for distribution of a biological sample that includes a substrate, wherein the substrate includes a plurality of sample chambers, a sample introduction channel for each sample chamber, and a venting channel for each sample chamber. The system may further include a preloaded reagent contained in each sample chamber and configured for nucleic acid analysis of a biological sample that enters the substrate and a sealing instrument configured to be placed in contact with the substrate to seal each sample chamber so as to substantially prevent sample contained in each sample chamber from flowing out of each sample chamber. The substrate can be constructed of detection-compatible and assay-compatible materials.
    Type: Grant
    Filed: May 27, 2010
    Date of Patent: December 3, 2013
    Assignee: Applied Biosystems, LLC
    Inventors: Min Yue, David M. Liu, Joy Roy, Yuh-Min Chiang, Joon Mo Yang, Dennis Lehto, Charles S. Vann, Nigel P. Beard, Ian A. Harding, John R. Van Camp, Alexander Dromaretsky, Sergey V. Ermakov, Mark F. Oldham, Maryam Shariati, Umberto Ulmanella
  • Patent number: 8551770
    Abstract: Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging. Oxygen diffusion occurs on both sides of the culture vessel, top and bottom. A gas-permeable membrane that includes perfluorocarbons in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which the cell culture apparatus is incubated.
    Type: Grant
    Filed: July 9, 2007
    Date of Patent: October 8, 2013
    Assignee: University of Miami
    Inventors: Christopher A. Fraker, Juan Dominguez-Bendala, Ricordi Camillo, Luca Inverardi
  • Patent number: 8551771
    Abstract: A gel molding apparatus is adapted to be used in combination with a vessel that has a plurality of solution chambers, and includes a lid plate and a plurality of columns that project from a top surface of the lid plate. Each of the columns has a bottom side connected to the top surface of the lid plate, a top side opposite to the bottom side, and a well recessed from the top side for receiving a gel suspension and having a depth from the top side. A method for gel molding is conducted via the gel molding apparatus. A culture apparatus includes the vessel and the gel molding apparatus. A method for culture is performed through the culture apparatus.
    Type: Grant
    Filed: December 10, 2009
    Date of Patent: October 8, 2013
    Assignee: Chung Gung University
    Inventors: Min-Hsien Wu, Wan-Chen Tsai
  • Patent number: 8518692
    Abstract: Gas permeable devices and methods are disclosed that provide highly efficient cell culture. Gas compartments, made up at least in part of gas permeable material, are dispersed within the culture device in locations that allow cells to remain within a fixed distance from a gas transmission location as the device scales in the horizontal direction. Gas permeable walls of the gas compartment(s) allow gas exchange with the ambient gas. Such an arrangement provides many advantages including the ability to eliminate the need for a gas-liquid interface, allow cell culture to proceed in the static mode (i.e. absent the need for media or gas to be pumped through the device), allow the scale of the device to increase in both the horizontal direction and vertical direction, reduce the rate of media evaporation, allow uncomplicated and low cost device fabrication, and provide the capacity for reduced feeding frequency.
    Type: Grant
    Filed: July 8, 2009
    Date of Patent: August 27, 2013
    Assignee: Wilson Wolf Manufacturing Corporation
    Inventor: John R. Wilson
  • Patent number: 8513000
    Abstract: Transfecting biology cells with nucleic acid molecules (DNA, siRNA) is an essential prerequisite in elucidating how genes function in complex cellular context and how their activities could be modulated for therapeutic intervention. Traditionally studies are carried out on a low throughput gene-by-gene scale, which has created a huge bottleneck in functional genomic study and drug discovery. Development of high-throughput cell transfection technology will permit functional analysis of massive number of genes and how their activities could be modulated by chemical or biological entities inside cells. This invention describes design, construction of device and apparatus for high throughput effective cell transfection. Procedures and protocols for using the device and apparatus are also described in the application. Novel methods of using the device in cell-based assays are also disclosed.
    Type: Grant
    Filed: April 15, 2010
    Date of Patent: August 20, 2013
    Assignee: Rational Biotechnology, Inc.
    Inventor: Yong Huang
  • Patent number: 8512974
    Abstract: The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for analyzing cell invasion.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: August 20, 2013
    Assignee: Platypus Technologies, LLC
    Inventors: Christopher Murphy, Barbara Israel, Nicholas Abbott
  • Patent number: 8507265
    Abstract: Transfecting biology cells with nucleic acid molecules (DNA, siRNA) is an essential prerequisite in elucidating how genes function in complex cellular context and how their activities could be modulated for therapeutic intervention. Traditionally studies are carried out on a low throughput gene-by-gene scale, which has created a huge bottleneck in functional genomic study and drug discovery. Development of high-throughput cell transfection technology will permit functional analysis of massive number of genes and how their activities could be modulated by chemical or biological entities inside cells. This invention describes design, construction of device and apparatus for high throughput effective cell transfection. Procedures and protocols for using the device and apparatus are also described in the application. Novel methods of using the device in cell-based assays are also disclosed.
    Type: Grant
    Filed: February 16, 2010
    Date of Patent: August 13, 2013
    Assignee: Rational Biotechnology, Inc.
    Inventor: Yong Huang
  • Patent number: 8486692
    Abstract: A cell culture apparatus consists of a vessel or array of vessels, each comprising a substantially flat bottom and substantially vertical sides, and composed of a substantially gas permeable material. The inner bottom surface of the vessel is commonly textured to provide the cells with adequate features for attachment and spreading. The vessel may include an integral annular flange which can be used to suspend the vessel from a suspensory element of a rack structure. To promote cell attachment and growth, the inner surfaces of the vessel bottom and sides may be coated with commonly available bio-active materials. Cells cultured in the system commonly grow in three dimensions for extended periods of time, and often produce significantly higher quantities of cellular products than cells grown in conventional labware.
    Type: Grant
    Filed: November 14, 2007
    Date of Patent: July 16, 2013
    Assignee: Acme Biosystems LLC
    Inventor: Eric M. Simon
  • Publication number: 20130177929
    Abstract: The present invention relates to a new three-dimensional co-culture method of podocytes and endothelial cells, and a relative co-culture system. Furthermore, the invention relates to the use of said co-culture system as an in vitro study model of pathologies affecting the kidneys, and in particular the renal glomerular filtration barrier.
    Type: Application
    Filed: December 16, 2010
    Publication date: July 11, 2013
    Applicant: FONDAZIONE IRCCS CA' GRANDA-OSPENDALE MAGGIORE POLICLINICO
    Inventors: Maria Pia Rastaldi, Min Li
  • Publication number: 20130143254
    Abstract: An insert device and co-culture device assembly incorporating the insert device, which includes an insert chamber with two fluid impermeable side walls that extend from a microporous bottom to an open top to form an inner cavity. The first side wall is shaped to form a convex arc that follows between about half of a circumference of the well and less than an entire circumference of the well. The second side wall joins ends of the first side wall and protrudes inward towards a center of the convex arc. A flange extends outward from or beneath the top and is notched to form a gap adjacent to the second side wall, which forms an access port allowing access to the lower chamber with a micropipette tip when the insert device is inserted into a well of a single or multi-well plate.
    Type: Application
    Filed: December 1, 2011
    Publication date: June 6, 2013
    Applicants: ROCHE DIAGNOSTICS OPERATIONS, INC., ACEA BIOSCIENCES, INC.
    Inventors: Donald Thomas, Wei Li, Ning Ke, Nan Li, Adrian Papas, Manfred Watzele, Alexander Seiler, Tianxing Wang, Yama Abassi, Xiaobo Wang
  • Publication number: 20130059339
    Abstract: A bioreactor (1) for the culture of cells (C) comprising a stack of carriers (7) for cell (C) adherence and liquid medium (M) distribution. The carriers (7) are stacked so as to define levels (6) between adjacent carriers (7) for the flow of the liquid medium (M). Adjacent levels (6) are fluidly interconnected via open spaces (2) so that the liquid medium (M) can flow from one level (6) to an adjacent level (6). The open spaces (2) between a first and an adjacent second level (6?) do not overlap with the one or more open spaces (2) between the second level (6?) and an adjacent third level (6?). One or more of the carriers may also include an area adapted to prevent cell adhesion or growth, thereby allowing for the viewing of cell growth on adjacent carriers from a vantage point external to the bioreactor. Related methods are also disclosed.
    Type: Application
    Filed: May 11, 2011
    Publication date: March 7, 2013
    Inventors: Didier Karerangabo, Julien Cardon, Jose Antonio Castillo Gonzalez
  • Patent number: 8309347
    Abstract: Cell expansion systems and methods of use are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second circulation loops (intracapillary loops and extracapillary loops) associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. Detachable flow circuits and methods of expanding cells are also provided.
    Type: Grant
    Filed: March 5, 2008
    Date of Patent: November 13, 2012
    Assignee: Terumo BCT, Inc.
    Inventor: Glen Delbert Antwiler
  • Patent number: 8268614
    Abstract: The present invention relates to the field of assays of cell movement. In particular, the present invention provides methods for assaying cell movement where inserts are used to confine cells to a defined area in a well of a multiwell plate and movement from the defined area is assayed.
    Type: Grant
    Filed: November 10, 2004
    Date of Patent: September 18, 2012
    Assignee: Platypus Technologies, LLC
    Inventors: Christopher Murphy, Barbara Israel, Nicholas Abbot
  • Patent number: 8241867
    Abstract: An integrated filtration and detection device for collecting and detecting the growth of microorganisms in a specimen includes a container defining a chamber therein. The container has an inlet and an outlet in fluid communication with the chamber. A filter is mounted in the chamber between the inlet and the outlet. A sensor is mounted in the chamber. The sensor is operative to exhibit a change in a measurable property thereof upon exposure to changes in the chamber due to microbial growth.
    Type: Grant
    Filed: August 24, 2010
    Date of Patent: August 14, 2012
    Assignee: bioMérieux, Inc.
    Inventors: James L. DiGuiseppi, Diederik Engbersen, Scott R. Jeffrey, John Walsh
  • Patent number: 8227240
    Abstract: An apparatus for culturing a bio-artificial tissue including a multi-well plate and a scaffold is disclosed herein. Also disclosed is a tissue indentation system that includes a probe, an isometric force transducer, a computer and a computer-controlled motor. A tissue response system is provided and has a well including a scaffold with a bio-artificial tissue suspended on the scaffold and the tissue indentation system.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: July 24, 2012
    Assignee: The Washington University
    Inventors: Elliot Elson, William B. McConnaughey, Tetsuro Wakatsuki
  • Patent number: 8222027
    Abstract: An auto-nucleating device includes a tube containing a crystalline cholesterol matrix. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within, and an opposite end that is initially open to receive the liquid. Another vessel includes an adaptor mounted to liquid container with a tubular branch closed by a needle septum and another tubular branch provided with a barbed fitting for engaging a flexible tube that terminates in a needle septum. In another embodiment, the vessel includes an inlet and vent branch at the top of the container and an outlet septum at a bottom opening.
    Type: Grant
    Filed: December 17, 2008
    Date of Patent: July 17, 2012
    Assignee: Cook General Biotechnolgy, LLC
    Inventors: Erik John Woods, John K. Critser
  • Patent number: 8216828
    Abstract: A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.
    Type: Grant
    Filed: September 16, 2008
    Date of Patent: July 10, 2012
    Assignee: Corning Incorporated
    Inventors: Henry J. Cattadoris, Gregory R. Martin, Allison J. Tanner
  • Patent number: 8168432
    Abstract: Gas permeable devices and methods are disclosed for cell culture, including cell culture devices and methods that contain medium at heights, and certain gas permeable surface area to medium volume ratios. These devices and methods allow improvements in cell culture efficiency and scale up efficiency.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: May 1, 2012
    Assignee: Wilson Wolf Manufacturing
    Inventors: John R. Wilson, Douglas A. Page, Dan Welch, Alison Robeck
  • Patent number: 8163540
    Abstract: A petri dish includes a cover and base, the cover having at least one circular opening in the top of the cover. A number of securing members are connected to the underside of the cover around the opening to secure at least one filter under the opening, the filter(s) being used to prevent any contaminants from the air from entering the petri dish. A guard member such as a plastic disc is disposed under the filter(s) to prevent any oil in the base of the petri dish from contaminating the filter(s). A sealing member is provided on a peripheral edge of the base to seal the petri dish when the petri dish is closed.
    Type: Grant
    Filed: July 18, 2008
    Date of Patent: April 24, 2012
    Inventor: Carlo Acosta