Bioreactor Patents (Class 435/289.1)
  • Patent number: 10792588
    Abstract: An organic material production system includes: a pretreatment device (12) that pulverizes a biomass material (11); a hydrothermal decomposition apparatus (14) that hydrothermally decomposes a pulverized biomass (13) by causing it to countercurrently contact with hot compressed water (15), elutes lignin components and hemicellulose components into the hot compressed water (15), and separates the lignin components and the hemicellulose components from a biomass solid residue; a first enzymatic hydrolysis device (19-1) that treats cellulose in a biomass solid residue (17), discharged from the hydrothermal decomposition apparatus, with an enzyme (18) to enzymatically hydrolyze it to a sugar solution containing hexose; a fermenter (21) that produces ethanol by fermentation using a sugar solution (20) obtained by the first enzymatic hydrolysis device (19-1); and a refiner 25 that refines an alcohol fermentation liquid (22), so as to separate it into ethanol (23) and a residue (24).
    Type: Grant
    Filed: September 19, 2008
    Date of Patent: October 6, 2020
    Assignees: MITSUBISHI HITACHI POWER SYSTEMS ENVIRONMENTAL SOLUTIONS, LTD., NATIONAL INSTITUTE OF ADVANCED SCIENCE AND TECHNOLOGY, INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
    Inventors: Minoru Genta, Ryosuke Uehara, Kinya Fujita, Tsuyoshi Sakaki, Noriyuki Yamada, Katsuichi Saito, Naoto Hashimoto, Yasuhiro Hasa
  • Patent number: 10775401
    Abstract: Aspects of the present disclosure include sample analysis methods and systems. According to certain embodiments, provided are methods of analyzing samples in an automated sample analysis system. The methods include introducing samples and sample preparation cartridges into the system, isolating and purifying an analyte (e.g., nucleic acids and/or proteins) present in the samples at a sample preparation station, and performing analyte detection assays in assay mixtures that include the purified analyte. Also provided are automated sample analysis systems that find use, e.g., in performing the methods of the present disclosure. In certain aspects, the methods and systems provide for continuous operator access during replenishment or removal of one or any combination of samples, bulk fluids, reagents, commodities, waste, and/or the like.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: September 15, 2020
    Assignee: Abbott Molecular Inc.
    Inventors: Jason E. Bryant, Daniel P. Casazza, Daniel J. Harris, Brett W. Johnson, Sonal Sadaria Nana, Timothy J. Patno, Frank Pawlowski, David M. Peters, Joseph P. Skinner, Mark Talmer
  • Patent number: 10758575
    Abstract: Provided herein are novel methods and composition utilizing adipose tissue-derived stromal stem cells for treating fistulae.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: September 1, 2020
    Assignees: TiGenix, S.A.U., UNIVERSIDAD AUTÓNOMA DE MADRID
    Inventors: María Gema Fernández Miguel, Manuel Ángel González De La Peña, Rosa Ana García Castro, Mariano García Arranz, Damián García Olmo
  • Patent number: 10755058
    Abstract: An information management system, comprising a first vessel having a first memory that stores first information, a second vessel having a second memory that stores second information, a detection circuit capable of detecting a proximity signal corresponding to approach of the second vessel to the first vessel, and a processor that adds the first information to the second memory that stores the second information, based on the proximity signal that has been detected by the detection circuit.
    Type: Grant
    Filed: November 6, 2018
    Date of Patent: August 25, 2020
    Assignee: OLYMPUS CORPORATION
    Inventors: Sumio Kawai, Wataru Hashimoto
  • Patent number: 10748278
    Abstract: An organism evaluation system for analyzing organisms within a fluid flow, comprising one or more of a stimulation section comprising a means for inducing a motive response in a living organism within the fluid flow passing through the stimulation section and a shepherding section comprising a means for temporarily separating such an organism from and then returning it to the fluid flow, a flow normalizing section in fluid communication with the stimulation section and/or shepherding section, and a viewing section in fluid communication with the flow normalizing section, the viewing section comprising a body having formed therein a body cavity defining a viewing port, the viewing section further comprising an optical system mounted relative to the body for viewing the fluid flow within the viewing port through a cavity first opening, whereby image data relating to the fluid flow and organisms therein is acquired via the optical system for analysis.
    Type: Grant
    Filed: May 8, 2017
    Date of Patent: August 18, 2020
    Assignee: SoBru Solutions, Inc.
    Inventor: John Miles Brubacher
  • Patent number: 10738270
    Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.
    Type: Grant
    Filed: March 13, 2020
    Date of Patent: August 11, 2020
    Assignee: The University of British Columbia
    Inventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
  • Patent number: 10704017
    Abstract: A cell culture carrier module and a cell culture system having the same are provided. A cell tank and a culture medium module respectively communicate with the carrier module. The carrier module includes a reactor, a first fixer, a second fixer and a plurality of cell culture carriers. The reactor has a chamber and at least one inlet/outlet. The inlet/outlet communicates with the chamber. The first fixer is fixed to the reactor and located in the chamber. The second fixer is disposed in the chamber and is movable relative to the first fixer. Two ends of each cell culture carrier are fixed to the first fixer and the second fixer, respectively. The cell culture carriers are in an untwisted state or a twisted state according to a variation in a distance between the first fixer and the second fixer due to a movement of the second fixer.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: July 7, 2020
    Assignee: Industrial Technology Research Institute
    Inventors: Ying-Wen Shen, Ing-Kae Wang, Chia-Jung Lu, Yea-Tzy Deng, Yu-Bing Liou, Sing-Ying Hsieh, Hsin-Hsin Shen, Hsiu-Ying Wang
  • Patent number: 10661275
    Abstract: Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: May 26, 2020
    Assignee: EMULATE, INC.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Joshua Gomes, Kyung Jin Jang
  • Patent number: 10662585
    Abstract: It is an object of the present invention to provide a method for producing ultrafine fibrous cellulose, which is capable of efficiently obtaining ultrafine fibrous cellulose having phosphoric acid groups with a high yield. The present invention relates to a method for producing fibrous cellulose having a fiber width of 1000 nm or less, comprising: a (A) of introducing phosphoric acid groups into cellulose fibers to form crosslinked structures via the phosphoric acid groups, thereby obtaining crosslinked phosphorylated cellulose fibers, a (B) of breaking some or all of the crosslinked structures to obtain crosslink-broken phosphorylated cellulose fibers, and a (C) of performing a mechanical treatment on the crosslink-broken phosphorylated cellulose fibers to obtain fibrous cellulose having a fiber width of 1000 nm or less, wherein, in the (A), crosslinked structures in an amount of 0.05 mmol/g or more and 2.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: May 26, 2020
    Assignee: OJI HOLDINGS CORPORATION
    Inventors: Yuichi Noguchi, Ikue Homma, Yusuke Matsubara
  • Patent number: 10626435
    Abstract: Nanofluidic devices and methods of the invention are capable of autonomously isolating individual microbial cells using constrictive channels and growing monocultures of the cells for automated characterization of their biochemical properties and interactions with mammalian cells. Single microbial cells, such as bacterial cells, are isolated directly from environmental sources and cultured using chemical factors from their native environment.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: April 21, 2020
    Assignee: Northeastern University
    Inventors: Edgar Daniel Goluch, Yoshiteru Aoi, Slava Epstein
  • Patent number: 10570360
    Abstract: A method of testing an analyte can include using a cell culture device that includes: at least three distinct chambers between the top wall and bottom wall. The chambers can include: an internal chamber defined by at least one porous internal wall; one or more boundary layer chambers having at least an inner boundary layer chamber defined by the at least one porous internal wall and at least one porous inner boundary layer wall, the at least one porous internal wall having a plurality of pores fluidically coupling the central internal chamber to the one or more boundary layer chamber; and an outer chamber.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: February 25, 2020
    Assignee: CFD RESEARCH CORPORATION
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant
  • Patent number: 10457906
    Abstract: An apparatus to prevent bio-filming during the delivery of photonic energy to a culture or set of organisms in a fluid culture medium, the device including a culture medium, a container configured to house the culture medium, and an assembly to generate and deliver a liquid energy transfer medium between the culture medium and the photonic device, the assembly including one or more apertures sized and shaped to form one or more liquid replenishing lenses and a fluid-light unit in the container to house the photonic device and the liquid energy transfer medium, the photonic device is at least one from among a photoreceptor, a photoemitter, or a combination of the photoreceptor and the photoemitter, and further including a pump coupled to the fluid-light unit, the assembly is configured to convey the photonic energy between the photonic device and the culture medium through the liquid energy transfer medium.
    Type: Grant
    Filed: April 14, 2015
    Date of Patent: October 29, 2019
    Assignee: Staterra LLC
    Inventors: Jared P. Green, Eric R. Brooks
  • Patent number: 10435658
    Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: October 8, 2019
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones
  • Patent number: 10435663
    Abstract: This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: October 8, 2019
    Assignee: ABEC, INC.
    Inventor: Cameron Knight
  • Patent number: 10427155
    Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: October 1, 2019
    Assignee: Illumina, Inc.
    Inventors: Alex Aravanis, Boyan Boyanov, M. Shane Bowen, Dale Buermann, Alexander Hsiao, Behnam Javanmardi, Tarun Khurana, Poorya Sabounchi, Hai Quang Tran
  • Patent number: 10364410
    Abstract: One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: July 30, 2019
    Assignee: Massachusetts Institute of Technology
    Inventor: Andreas Mershin
  • Patent number: 10330585
    Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: June 25, 2019
    Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
    Inventors: Osamu Koseki, Ryo Suenaga, Masahiro Kuninori
  • Patent number: 10273441
    Abstract: The invention relates to a perfusion manifold assembly that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with an assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. The invention further relates to a drop-to-drop connection scheme for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: April 30, 2019
    Assignee: EMULATE, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Guy Robert Thompson, II, Petrus Wilhelmus Martinus van Ruijven, Matthew Daniel Solomon, Christian Alexander Potzner, Patrick Sean Tuohy, Joshua Gomes, Norman Wen, Jacob Freake, Doug Sabin
  • Patent number: 10233416
    Abstract: A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: March 19, 2019
    Assignee: EMULATE, INC.
    Inventors: Christopher David Hinojosa, Guy Robert Thompson, II, Joshua Gomes, Jacob Freake, Doug Sabin
  • Patent number: 10184102
    Abstract: A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: January 22, 2019
    Assignee: Emulate, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Joshua Gomes, Jose Fernandez-Alcon
  • Patent number: 10179901
    Abstract: The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: January 15, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Clark K. Colton, Jeffrey R. Millman
  • Patent number: 10175140
    Abstract: The present invention provides a portable luminescence detector combined with a mobile device, which mainly comprises a body, a focusing lens module, a light source module, a sample tray, and an image capturing module, wherein the image capturing module can be a lens of the mobile device, the body can be disassembled into multiple parts to act as holders for the portable luminescence detector. When the portable luminescence detector of the present invention is used in conjunction with the mobile device, the sample tray is placed at a position corresponding to that of the focusing lens module, and the light source module is used for providing a light source band, then the image capturing module works with the focus lens module to capture an image of the sample under the light source band and to provide the image for RGB spectral analysis or real-time image RGB spectral analysis.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: January 8, 2019
    Assignee: SOOCHOW UNIVERSITY
    Inventors: Mei-Lin Ho, Chun-Yen Lin, Wei-Ren Lin, Ying-Chu Wang, Kuen-Yuh Chung
  • Patent number: 10125342
    Abstract: Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: November 13, 2018
    Assignee: EMULATE, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Guy Robert Thompson, II, Petrus Wilhelmus Martinus van Ruijven, Matthew Daniel Solomon, Christian Alexander Potzner, Patrick Sean Tuohy
  • Patent number: 10092906
    Abstract: Disclosed are cartridges and modules that may be utilized in diagnostic systems and methods. The cartridge includes a flexible seal that caps the cartridge. The flexible seal has an opening for a pipette tip, and the flexible seal is configured to create a sealed environment when a pipette tip is positioned in the opening of the flexible seal and when the pipette tip is moved in the X-axis, Y-axis, and Z-axis.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: October 9, 2018
    Assignee: LUMINEX CORPORATION
    Inventors: Tom Westberg, Bruce Richardson, Ryan Griswold, Chris Lewis
  • Patent number: 10093953
    Abstract: A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: October 9, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Vijayanand Suryakant Moholkar
  • Patent number: 10093540
    Abstract: According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the liquid hydrocarbon with the renewable hydrogen or alternatively, the renewable hydrogen can be added to a reactor operated so as to simultaneously desulfurize and hydrogenate the hydrocarbons. The present invention enables a party to receive a renewable fuel credit for the transportation or heating fuel.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: October 9, 2018
    Assignee: Iogen Corporation
    Inventor: Brian Foody
  • Patent number: 10088398
    Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: October 2, 2018
    Assignee: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, John Doyle
  • Patent number: 10087414
    Abstract: A continuous device for culturing mammalian cells in a three-dimensional structure for the transplantation or implantation in vivo is described. The culturing device comprises (a) a scaffold formed by a matrix of interconnected growth surfaces spaced at regular intervals and (b) a fluid distribution means at the inlet and the exit of the growth areas. The device is particularly useful for culturing bone cells for dental implants or bone reconstruction.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: October 2, 2018
    Assignee: VIVABIOCELL, S.p.A.
    Inventors: Eric Berry, Francesco Curcio
  • Patent number: 10066198
    Abstract: A device for in-vitro modelling in-vivo tissues of organs that includes a first body portion with at least one access chamber, a second body portion with at least one culturing chamber, and a culturing membrane dividing the at least one access chamber from the culturing chamber. The device further includes a third body portion with at least one actuation chamber having at least one limitation cavity, and an actuation membrane dividing the at least one culturing chamber from the at least one actuation chamber. With the device, a robust actuation system can be provided that does not depend on the mechanical properties of the actuation membrane material, nor on the pressure, and that allows to mimic three-dimensional deformations of the tissue, in particular of lung alveoli.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: September 4, 2018
    Assignee: Universität Bern
    Inventors: Olivier Thierry Guenat, Marcel Felder, Andreas Stucki, Janick Daniel Stucki, Thomas Geiser
  • Patent number: 10030254
    Abstract: The present invention provides a method of producing hydrogen from a waste material, comprising steps of fermenting 100 a fermentation mixture comprising the waste material in a reactor 1 with a headspace 1a under anaerobic conditions, removing 200 hydrogen from a gas from the headspace 1a during fermentation to produce a hydrogen gas and a remainder gas and recirculating 300 at least a portion of the remainder gas back to the headspace 1a. An apparatus for producing hydrogen and recirculating at least a portion of the remainder gas to the headspace 1a is also provided.
    Type: Grant
    Filed: September 2, 2015
    Date of Patent: July 24, 2018
    Assignee: Drexel University
    Inventor: Charles Nathan Haas
  • Patent number: 10022720
    Abstract: A fluidic device for a cartridge for testing biological samples is disclosed herein. In an embodiment, the fluidic device includes a fluidic chamber, at least one microfluidic channel in fluid communication with the fluidic chamber, a venting port configured to apply a pneumatic force to the fluidic chamber, at least one passive valve located within the at least one microfluidic channel and configured to allow or stop fluid flow through the at least one microfluidic channel based on a pressure difference, and a controller configured to control the pneumatic force applied to the fluidic chamber via the venting port.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: July 17, 2018
    Assignee: CYTOCHIP INC.
    Inventors: Wendian Shi, Yuzhe Ding
  • Patent number: 10004185
    Abstract: A closed loop compost tea brewing system with a hydroponic reservoir and a compost tea brewing assembly configured to brew compost tea. The compost tea brewer assembly includes a tea brewing reservoir. The system includes has a closed loop arrangement fluidly connecting the tea brewing reservoir and the hydroponic reservoir. The closed loop arrangement is configured to provide continuous fluid flow from the tea brewing reservoir to the hydroponic reservoir, which is recirculated back from the hydroponic reservoir to the tea brewing reservoir.
    Type: Grant
    Filed: October 21, 2015
    Date of Patent: June 26, 2018
    Inventor: Matthew Fingerle
  • Patent number: 10005997
    Abstract: The present invention relates to a port installed in a closed-ended photochemical reactor, and more particularly, to a multi-functional port for microalgae cultivation and harvesting that supplies gas for cultivation for culturing microalgae by connecting to the closed-ended photochemical reactor; supplies gas for prevention of precipitation for lifting microalgae during cultivation; and assembles by taking samples of microalgae or selectively combining a plurality of valves which can harvest microalgae, whose cultivation is completed.
    Type: Grant
    Filed: June 1, 2016
    Date of Patent: June 26, 2018
    Assignee: KOREA DISTRICT HEATING CORP.
    Inventors: Won Seok Chang, Deog Yong Ahn, Sang Ho Shin, Ji Hye Yu, Young Jae Lee, Won Kwon Lee, Kwang Keun Choi, Sung Min Kim
  • Patent number: 10006002
    Abstract: A continuous device for culturing mammalian cells in a three-dimensional structure for the transplantation or implantation in vivo is described. The culturing device comprises (a) a scaffold formed by a matrix of interconnected growth surfaces spaced at regular intervals and (b) a fluid distribution means at the inlet and the exit of the growth areas. The device is particularly useful for culturing bone cells for dental implants or bone reconstruction.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: June 26, 2018
    Assignee: VIVABIOCELL S.P.A.
    Inventor: Francesco Curcio
  • Patent number: 9988651
    Abstract: A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: June 5, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Maxim A. Promtov, Vijayanand Suryakant Moholkar
  • Patent number: 9950015
    Abstract: A portable apparatus for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with an internal containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. The apparatus has fluid suction features including a suction port and a conduit providing fluid communication from the suction port to a location within the filtrate volume within a tapered portion of the internal containment volume, and optionally including a second suction port and a second conduit to a different location in the filtrate volume with the second conduit being configured to permit elevation adjustment within the filtrate volume.
    Type: Grant
    Filed: September 23, 2015
    Date of Patent: April 24, 2018
    Assignee: The GID Group, Inc.
    Inventors: William W. Cimino, Adam J. Katz, Ramon Llull
  • Patent number: 9944964
    Abstract: A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: April 17, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Maxim A. Promtov, Vijayanand Suryakant Moholkar
  • Patent number: 9938493
    Abstract: A method for producing a single-use cell culture container at least partially made of polymer material, wherein the cell culture container is treated with gamma radiation for sterilization, characterized in that the cell culture container is treated with inert gas and/or a defined amount of oxygen before the irradiation in order to prevent the occurrence of an “extended lag phase”.
    Type: Grant
    Filed: May 23, 2013
    Date of Patent: April 10, 2018
    Assignee: SARTORUIS STEDIM BIOTECH GMBH
    Inventors: Wolfgang Ludwig Erl, Joseph Seidl
  • Patent number: 9932562
    Abstract: The invention provides a method of increasing product yield per culture in a population of product-secreting cells bound to a scaffold, for example, a microcarrier, at least partially immersed in a culture medium in a bioreactor. The method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration while leaving the scaffold with the bound cells in the bioreactor; re-feeding the cells by adding a fresh culture medium to increase the culture medium in the bioreactor to approximately the original volume; agitating the culture medium to allow the cells to grow and release a second-secreted product concentration; harvesting product by removing the culture medium with the second-secreted product concentration while leaving the scaffold with the bound cells behind. Also disclosed is a method of increasing virus yield per culture in a population of virus-infected cells bound to a microcarrier suspended in a culture medium.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: April 3, 2018
    Assignee: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: Patrick M. Guertin, Joseph D. Crowell
  • Patent number: 9932550
    Abstract: A cell culture device can include: at least 3 distinct chambers between the top wall and bottom wall. The perimeter walls can include: an internal chamber defined by at least one porous internal wall; one or more boundary layer chambers having at least an inner boundary layer chamber defined by the at least one porous internal wall and at least one porous inner boundary layer wall, the at least one porous internal wall having a plurality of pores fluidically coupling the central internal chamber to the one or more boundary layer chamber; and an outer chamber.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: April 3, 2018
    Assignee: CFD RESEARCH CORPORATION
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant
  • Patent number: 9909094
    Abstract: A portable apparatus for collection and processing of human biological material, such as containing adipose extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. The apparatus includes a mixing device disposed at least in part within the tissue retention volume, and wherein the mixing device has a rotatable shaft extending from outside of to inside of the internal containment volume and has at least one mixing member that is disposed in the tissue retention volume with at least a portion disposed in the tapered portion of the internal containment volume and that is connected with the shaft to move through the tissue retention volume when the shaft is rotated.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: March 6, 2018
    Assignee: The GID Group, Inc.
    Inventors: William W. Cimino, Adam J. Katz, Ramon Llull
  • Patent number: 9873121
    Abstract: A system for connecting a reagent container to a socket. The container has a cap with an outlet comprising a collar having an opening on its side, the collar surrounding a spout with a movable valve. The valve has a tube surrounded by a disk. A plug is centrally arranged within one end of the tube so the outer walls of the spout form-fit with the inner side of the tube and the inner walls of the spout form-fit with the outer side of the plug for closing the spout. The plug is connected to one end of the tube and a socket for accommodating the outlet of the container's cap. The socket comprises at least two movable levers to engage into openings of the collar and a socket gasket on one side for taking up the tube of the valve and tubing on the opposite side to drain liquids.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: January 23, 2018
    Assignee: STRATEC Biomedical AG
    Inventors: Martin Trump, Thomas Lowien, Matthias Barth
  • Patent number: 9752164
    Abstract: Overlay processes are disclosed for making ethanol that not only increase ethanol conversion but do so in a cost effective manner with a reduction in energy requirements per unit of ethanol production. The processes can provide, if desired, higher organic compound as a co-product with ethanol.
    Type: Grant
    Filed: June 14, 2013
    Date of Patent: September 5, 2017
    Assignee: MICROVI BIOTECH, INC.
    Inventors: Fatemeh Razavi-Shirazi, Ameen Razavi, Norman Louis Balmer
  • Patent number: 9744507
    Abstract: A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.
    Type: Grant
    Filed: November 5, 2013
    Date of Patent: August 29, 2017
    Assignee: EMD Millipore Corporation
    Inventors: Martin Morrissey, Brian Pereira
  • Patent number: 9725687
    Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: August 8, 2017
    Assignees: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, VANDERBILT UNIVERSITY
    Inventors: John P. Wikswo, Philip C. Samson, Frank Emmanuel Block, III, Ronald S. Reiserer, Kevin Kit Parker, John A. McLean, Lisa Joy McCawley, Dmitry Markov, Daniel Levner, Donald E. Ingber, Geraldine A. Hamilton, Josue A. Goss, Robert Cunningham, David E. Cliffel, Jennifer Robin McKenzie, Anthony Bahinski, Christopher David Hinojosa
  • Patent number: 9695372
    Abstract: Provided herein are compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to effect production, expression, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.
    Type: Grant
    Filed: September 10, 2008
    Date of Patent: July 4, 2017
    Assignee: Sapphire Energy, Inc.
    Inventors: Jason Pyle, Alex Aravanis
  • Patent number: 9670448
    Abstract: A bioreactor has a reactor bag (12) with a predominantly flexible bag wall (121) that has a rigid bearing area. A stirrer arrangement (14) is in the bearing area and includes rigid profile ring (16) made of a non-magnetic material and fixed in the bearing area. The stirrer arrangement (14) also includes a shaftless stirrer (18) rotatably engaged with the profile ring (16) and having parts that are permanently magnetic. An electric coil arrangement (20) is outside the reactor bag (12) and can produce a rotating magnetic field that interacts with the permanent-magnetic areas of the stirrer (18) to produce a torque that rotates the stirrer (18) and circulates the contents of the bag (12).
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: June 6, 2017
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Stefan Zeuch, Thomas Regen, Gerhard Greller, Oscar-Werner Reif
  • Patent number: 9623414
    Abstract: Individual temperature control in multiple reactions performed simultaneously in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawing heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: April 18, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jeff Ceremony, Daniel Y. Chu
  • Patent number: 9617521
    Abstract: Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.
    Type: Grant
    Filed: October 21, 2014
    Date of Patent: April 11, 2017
    Assignee: HemoShear, LLC
    Inventors: Brian R. Wamhoff, Brett R. Blackman, Robert A. Figler, Daniel G. Gioeli, Michael B. Simmers
  • Patent number: 9611496
    Abstract: A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step.
    Type: Grant
    Filed: December 9, 2013
    Date of Patent: April 4, 2017
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Vijayanand Suryakant Moholkar