Bioreactor Patents (Class 435/289.1)
  • Patent number: 11208626
    Abstract: The present invention provides a method for culturing cells in a cell culture container having a base section, a top section arranged in parallel with the top section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the container is composed of a flexible material, comprising culturing cells in a culture medium in the cell culture container.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: December 28, 2021
    Assignee: Ori Biotech Ltd.
    Inventors: Christopher Mason, Farlan Veraitch
  • Patent number: 11208627
    Abstract: A biocontainment vessel includes a vessel structure including a structural composition and an enhancement composition associated with the structural composition. The enhancement composition includes a co-polymer. The co-polymer is a poly(glycerol sebacate) or a poly(glycerol sebacate urethane). The enhancement composition may also include an augmentation agent associated with the co-polymer. The enhancement composition is located with respect to the structural composition such that the enhancement composition benefits biological cells contained in the biocontainment vessel. A composition includes a co-polymer and an augmentation agent contained by the co-polymer. A method of containing biological cells includes placing the biological cells in an augmented biocontainment vessel and storing them in the augmented biocontainment vessel under predetermined conditions. An augmented substrate includes a substrate and an enhancement composition coating a surface of the substrate.
    Type: Grant
    Filed: January 17, 2019
    Date of Patent: December 28, 2021
    Assignee: THE SECANT GROUP, LLC
    Inventors: Peter D. Gabriele, Jeremy J. Harris, Charles Brendan Nicholson, Steven Lu, Jeffrey H. Robertson, Gael Peron
  • Patent number: 11203019
    Abstract: Embodiments include a reaction vessel having a first reaction chamber filled with a first material; a first light absorbing region adhered to an interior-facing surface of the first reaction chamber; a second reaction chamber filled with a second material; a second light absorbing region adhered to an interior-facing surface of the second reaction chamber; a temperature sensor disposed within the second reaction chamber; and one or more energy sources configured to direct light at the first light absorbing region and the second light absorbing region. A processor may be employed to determine a first temperature of the first material from a second temperature of the second material measured by the temperature sensor. Methods of manufacturing such a reaction vessel are also disclosed.
    Type: Grant
    Filed: October 16, 2019
    Date of Patent: December 21, 2021
    Assignee: Kryptos Biotechnologies, Inc.
    Inventors: Jun Ho Son, Austin So
  • Patent number: 11198842
    Abstract: An in vitro microfluidic device includes a device configured to model a blood-brain barrier. The device includes a center well in fluidic communication with each of an inlet and an outlet. Each of the center well, inlet, and outlet includes a porous membrane that separates a “blood” portion (a fluid flow portion) from a “brain” portion (a fluid containing portion). The porous membrane is seeded with endothelial cells such as the human venule endothelial cells (HUVECs) on the blood side, and with astrocytes on the brain side, to accurately model the blood-brain barrier. Fluid flows between the inlet, the center well, and the outlet to test the permeability of the porous membrane, thereby providing an accurate in vitro model of a blood-brain barrier.
    Type: Grant
    Filed: February 24, 2020
    Date of Patent: December 14, 2021
    Assignee: University of South Florida
    Inventors: Subhra Mohapatra, Shyam S. Mohapatra, Tao Wang, Taylor Martinez
  • Patent number: 11192796
    Abstract: A co-assembly method for synthesizing inverse photonic structures is described. The method includes combining an onium compound with a sol-gel precursor to form metal oxide (MO) nanocrystals, where each MO nanocrystal has crystalline and amorphous content. The MO nanocrystals are combined with templating particles to form a suspension. A solvent is evaporated from the suspension to form an intermediate or compound product, which then undergoes calcination to produce an inverse structure.
    Type: Grant
    Filed: April 3, 2017
    Date of Patent: December 7, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Joanna Aizenberg, Tanya Shirman, Katherine Reece Phillips, Elijah Shirman, Theresa M. Kay
  • Patent number: 11174456
    Abstract: One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.
    Type: Grant
    Filed: July 30, 2019
    Date of Patent: November 16, 2021
    Assignee: Massachusetts Institute of Technology
    Inventor: Andreas Mershin
  • Patent number: 11174462
    Abstract: The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.
    Type: Grant
    Filed: April 17, 2018
    Date of Patent: November 16, 2021
    Assignees: EMULATE, Inc., Cedars-Sinai Medical Center
    Inventors: S. Jordan Kerns, Norman Wen, Carolina Lucchesi, Christopher David Hinojosa, Jacob Fraser, Geraldine Hamilton, Gad Vatine, Samuel Sanees, Clive Svendsen, Daniel Levner, Dhruv Sareen
  • Patent number: 11105824
    Abstract: The invention essentially consists in advantageously using and arranging at least one robot (8) relative to one or more storage recipients (4) and a transfer station (5) for transferring the containers to the unit in such a way as to take advantage of the flexibility of movement and grip offered by the arm or arms of the robot or robots and its/their gripping member, in order to automate all of the operations carried out manually by an operator in conventional biobanks.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: August 31, 2021
    Assignee: IRELEC
    Inventors: Matthieu Privat De Fortune, Gerard Marot, Aymeric Cunrath, Yohan Briand, Rattena Tang
  • Patent number: 11067571
    Abstract: This invention is in the field of surface modification. In particular, the invention relates to the surface modification of microfluidic devices to alter surface hydrophobicity characteristics.
    Type: Grant
    Filed: January 10, 2020
    Date of Patent: July 20, 2021
    Assignee: EMULATE, INC.
    Inventors: Daniel Levner, S. Jordan Kerns, Jefferson Puerta
  • Patent number: 11060058
    Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.
    Type: Grant
    Filed: June 5, 2015
    Date of Patent: July 13, 2021
    Assignee: Genzyme Corporation
    Inventors: Agata Villiger-Oberbek, Jianguo Yang, Yang Yang, Gabrielle Lorenzo
  • Patent number: 11054362
    Abstract: A system and method adapted to have high sensitivity to the formation of biofilm by mixed community bacteria in fluids. The system enhances corrosion imbalance by differentiating conditions between metal sensor elements immersed in the liquid being monitored. The liquid may be diverted to and flowed through a sample chamber where adjacent sensor elements may reside in different flow velocity or temperature regions. The differentiated conditions allow for different film formation on one of the sensor elements relative to the other, and also more quickly than in the main system from which the sampled liquid has been diverted. The differentiated formation allows the use of measurement of polarization current between the metal sensors to produce data with superior resolution relative to prior methods. The speed of film formation promoted by the differentiated conditions allows for determination of risk of film formation in the main system prior to that film's formation in the main system.
    Type: Grant
    Filed: July 21, 2017
    Date of Patent: July 6, 2021
    Inventor: David Robert Howland
  • Patent number: 11045396
    Abstract: Exemplary embodiments of the disclosure may be drawn to a device having a vessel configured to contain a source of lipids and a chamber fluidly connected to an outlet of the vessel. The chamber may contain immobilized lipase positioned within a flow path in the chamber along which the lipids flow when released from the vessel into the chamber. The device may also include an outlet through which the lipids flow after passing through the chamber.
    Type: Grant
    Filed: August 15, 2018
    Date of Patent: June 29, 2021
    Assignee: Alcresta Therapeutics, Inc.
    Inventors: Eric First, David Widom
  • Patent number: 11016007
    Abstract: The present invention describes an automated platform for inoculating a variety of receptacles with biological samples for testing and analysis. The lab automation system includes a plurality of modules used to automate the inoculation of media for subsequent analysis. In this regard, the lab automation system has one module to enter specimen/order information and store an inventory of petri dishes. Another module is used to label the sample receptacles with a unique identifier that associates the receptacles with the sample. Yet another module includes a robot for retrieving sample and inoculating the receptacles. The sample inoculation module also includes an apparatus that will receive slides, inoculate those slides, and further process the slides for analysis. Finally, the lab automation system includes a module that streaks the culture media with the sample. Thus, the automated lab system described herein provides consistent samples with minimal input from a lab operator.
    Type: Grant
    Filed: March 31, 2015
    Date of Patent: May 25, 2021
    Assignee: BD KIESTRA B.V.
    Inventors: Martijn Xander Berntsen, Martijn Kleefstra, Roger Petri, Tino Walter Van Der Zee
  • Patent number: 11015160
    Abstract: A filter holding device (1; 41; 75; 81) arranged for providing at least one filter (7; 43a, 43b; 85) inside a flexible bag (3; 71), said filter holding device comprising attaching means (19a-d, 21a-d; 79; 83) for attaching the filter holding device to an inner surface of the flexible bag such that the at least one filter held by the filter holding device is provided at a distance from the inner surface of the flexible bag.
    Type: Grant
    Filed: September 12, 2016
    Date of Patent: May 25, 2021
    Assignee: Cytiva Sweden AB
    Inventors: Eric Faldt, Ralph Stankowski
  • Patent number: 11001797
    Abstract: A system and methods for aerosol delivery of an entity or agent are disclosed. The system and methods can include a target application surface. A nebulizer can be located in close proximity to the target application surface. The nebulizer can include a chamber to hold the entity, a nozzle plate including one nozzle, and a piezoelectric element coupled to the nozzle plate. A power source can be coupled to the piezoelectric element. The power source, when activated, can energize the piezoelectric element to vibrate the nozzle plate to cause the entity to be nebulized through the nozzle to impact the target application surface.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: May 11, 2021
    Assignee: President and Fellows of Harvard College
    Inventors: Karel Domansky, Michael Karpelson, Donald E. Ingber
  • Patent number: 10981111
    Abstract: The present invention relates to a carbon dioxide conversion reactor and more particularly, to a carbon dioxide conversion reactor capable of converting carbon dioxide contained in flue gas into an aqueous bicarbonate solution that may be used in many applications; and at the same time, preventing back pressure from increasing due to supplied flue gas by allowing a conversion process to rapidly proceed, thereby significantly reducing the level of carbon dioxide contained in flue gas with high efficiency and high conversion speed, a series reactor for converting and capturing carbon dioxide including the carbon dioxide conversion reactor, and a process of converting and capturing carbon dioxide using the carbon dioxide conversion reactor.
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: April 20, 2021
    Assignee: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: Jungbae Kim, Han Sol Kim, Sung Gil Hong
  • Patent number: 10961496
    Abstract: A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
    Type: Grant
    Filed: December 2, 2016
    Date of Patent: March 30, 2021
    Assignee: EMULATE, INC.
    Inventors: Daniel Levner, Christopher David Hinojosa, Norman Wen, Antonio Varone, Justin Nguyen, Lina Williamson, S. Jordan Kerns, Catherine Karalis, Geraldine Hamilton, Carol Lucchesi
  • Patent number: 10961495
    Abstract: The cell structure producing apparatus including needles, a sticking unit configured to detachably hold an end portion of each of the needles, descend the each of the needles with respect to a cell tray in which cell aggregates are held, stick and penetrate each of the cell aggregates with the tip end, and ascend the needle after sticking, at least one time or more, a base unit, and a control unit configured to move the sticking unit so that for the each of the needles, each of the needles sticking the cell aggregates is positioned at a predetermined position over the base unit, descend each of the needles by a predetermined amount to stick the tip end into the base unit at the predetermined position on the base unit, and control the sticking unit.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: March 30, 2021
    Assignee: CYFUSE BIOMEDICAL K.K.
    Inventors: Yasuto Kishii, Norihiko Tokunaga
  • Patent number: 10960393
    Abstract: Apparatus and methods for providing a single-use, disposable module or manifold for testing and analysis of bioprocesses, as well as a portable and compact device which can be used with the module or manifold. A module comprising a valve, a filter or guard column, an affinity column, and a flow cell is provided with several ports for receiving tubing connections for a sample and one or more solvents, as well as one or more outlet ports for connections to one or more waste reservoirs. The flow cell may use UV light to determine a protein concentration of a sample in one particular example. The module can be connected directly or indirectly to a bioreactor containing the bioprocess and material to be sampled, and can be disposed of once a production run has been completed. In addition, manifolds are provided which can be embodied as a valve assembly, and which can comprise the same components and features as the disposable module. The manifolds and modules are compact and easy to use.
    Type: Grant
    Filed: August 21, 2018
    Date of Patent: March 30, 2021
    Assignee: IDEX HEALTH AND SCIENCE LLC
    Inventors: Victor Simonyi, Darrin Kurt Pickle, James Smyth, Craig Love
  • Patent number: 10954482
    Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: March 23, 2021
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Donald E. Ingber, Anthony Bahinski, Robert Cunningham, Josue A. Goss, Geraldine A. Hamilton, Christopher David Hinojosa, Daniel Levner, Kevin Kit Parker
  • Patent number: 10865374
    Abstract: An apparatus 100 for cultivating bacteria, comprising a conduit 101 having an upstream and a downstream section and the downstream section of the conduit 101 comprising first Venturi eductor means 111 with at least two inlet ports 117, 118 wherein one of said inlet ports 117 is in fluid communication with a supply of nutrient and another one of said inlet ports 118 is in fluid communication with a supply of bacteria such that, in use, nutrient and bacteria are drawn into said Venturi eductor means 111 by a fluid passing along said conduit 101 and said Venturi eductor means 111.
    Type: Grant
    Filed: July 20, 2017
    Date of Patent: December 15, 2020
    Assignee: Delaware Capital Formation, Inc.
    Inventors: David Fielding, Chris Dyer
  • Patent number: 10852296
    Abstract: Cell and organ (or tissue) cultures provide a micro-environment with respect to nutrients, gas exchange, and scaffolding in order to encourage specific cell function, and in some cases to mimic in-vivo cellular expression under in-vitro conditions. We describe apparatus and methods to chemically, spatially, and temporally measure diffusible molecules produced, or used by cells or tissues in culture. In this manner, mechanisms of cell-cell interaction and other chemical signaling, detailed biochemical pathways, and the action of potential pharmaco-therapy agents can be better understood at a molecular level. In addition to basic science, the technical advantages of process monitoring and control can be applied to optimize culture products in bioreactors. Embodiments of this device are intended to simulate and monitor [input and output] the behavior of vascular capillary beds in higher species vascular systems.
    Type: Grant
    Filed: December 27, 2012
    Date of Patent: December 1, 2020
    Assignee: BiOMICom Incorporated
    Inventors: James D. Buchner, Ross C. Willoughby
  • Patent number: 10844413
    Abstract: Disclosed herein are methods, systems, and compositions for the pretreatment of biomass within seconds with low inhibitor formation. The pretreatment process is used to convert biomass to a fuel or other useful chemicals by subjecting the feedstock to a rapid retention time under pressure and temperature and/or chemical reactant. The system includes a continuously-operating valve discharge apparatus to discharge pretreated feedstock while maintaining uniform pressure on the pretreatment system.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: November 24, 2020
    Assignee: Sweetwater Energy, Inc.
    Inventor: Robert E. Lumpkin
  • Patent number: 10828638
    Abstract: An in vitro microfluidic “organ-on-chip” is described herein that mimics the structure and at least one function of specific areas of the epithelial system in vivo. In particular, a multicellular, layered, microfluidic culture is described, allowing for interactions between lamina propria-derived cells and the associated tissue specific epithelial cells and endothelial cells. This in vitro microfluidic system can be used for modeling inflammatory tissue, e.g., autoimmune disorders involving epithelia and diseases involving epithelial layers. These multicellular, layered microfluidic “organ-on-chip”, e.g. “epithelia-on-chip” further allow for comparisons between types of epithelia tissues, e.g., lung (Lung-On-Chip), bronchial (Airway-On-Chip), skin (Skin-On-Chip), cervix (Cervix-On-Chip), blood brain barrier (BBB-On-Chip), etc., in additional to neurovascular tissue, (Brain-On-Chip), and between different disease states of tissue, i.e. healthy, pre-disease and diseased areas.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: November 10, 2020
    Assignee: Emulate, Inc.
    Inventors: S. Jordan Kerns, Riccardo Barrile, Geraldine Hamilton, Catherine Karalis, Daniel Levner, Carolina Lucchesi, Antonio Varone, Remi Villenave
  • Patent number: 10792588
    Abstract: An organic material production system includes: a pretreatment device (12) that pulverizes a biomass material (11); a hydrothermal decomposition apparatus (14) that hydrothermally decomposes a pulverized biomass (13) by causing it to countercurrently contact with hot compressed water (15), elutes lignin components and hemicellulose components into the hot compressed water (15), and separates the lignin components and the hemicellulose components from a biomass solid residue; a first enzymatic hydrolysis device (19-1) that treats cellulose in a biomass solid residue (17), discharged from the hydrothermal decomposition apparatus, with an enzyme (18) to enzymatically hydrolyze it to a sugar solution containing hexose; a fermenter (21) that produces ethanol by fermentation using a sugar solution (20) obtained by the first enzymatic hydrolysis device (19-1); and a refiner 25 that refines an alcohol fermentation liquid (22), so as to separate it into ethanol (23) and a residue (24).
    Type: Grant
    Filed: September 19, 2008
    Date of Patent: October 6, 2020
    Assignees: MITSUBISHI HITACHI POWER SYSTEMS ENVIRONMENTAL SOLUTIONS, LTD., NATIONAL INSTITUTE OF ADVANCED SCIENCE AND TECHNOLOGY, INCORPORATED ADMINISTRATIVE AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
    Inventors: Minoru Genta, Ryosuke Uehara, Kinya Fujita, Tsuyoshi Sakaki, Noriyuki Yamada, Katsuichi Saito, Naoto Hashimoto, Yasuhiro Hasa
  • Patent number: 10775401
    Abstract: Aspects of the present disclosure include sample analysis methods and systems. According to certain embodiments, provided are methods of analyzing samples in an automated sample analysis system. The methods include introducing samples and sample preparation cartridges into the system, isolating and purifying an analyte (e.g., nucleic acids and/or proteins) present in the samples at a sample preparation station, and performing analyte detection assays in assay mixtures that include the purified analyte. Also provided are automated sample analysis systems that find use, e.g., in performing the methods of the present disclosure. In certain aspects, the methods and systems provide for continuous operator access during replenishment or removal of one or any combination of samples, bulk fluids, reagents, commodities, waste, and/or the like.
    Type: Grant
    Filed: March 15, 2017
    Date of Patent: September 15, 2020
    Assignee: Abbott Molecular Inc.
    Inventors: Jason E. Bryant, Daniel P. Casazza, Daniel J. Harris, Brett W. Johnson, Sonal Sadaria Nana, Timothy J. Patno, Frank Pawlowski, David M. Peters, Joseph P. Skinner, Mark Talmer
  • Patent number: 10758575
    Abstract: Provided herein are novel methods and composition utilizing adipose tissue-derived stromal stem cells for treating fistulae.
    Type: Grant
    Filed: November 11, 2014
    Date of Patent: September 1, 2020
    Assignees: TiGenix, S.A.U., UNIVERSIDAD AUTÓNOMA DE MADRID
    Inventors: María Gema Fernández Miguel, Manuel Ángel González De La Peña, Rosa Ana García Castro, Mariano García Arranz, Damián García Olmo
  • Patent number: 10755058
    Abstract: An information management system, comprising a first vessel having a first memory that stores first information, a second vessel having a second memory that stores second information, a detection circuit capable of detecting a proximity signal corresponding to approach of the second vessel to the first vessel, and a processor that adds the first information to the second memory that stores the second information, based on the proximity signal that has been detected by the detection circuit.
    Type: Grant
    Filed: November 6, 2018
    Date of Patent: August 25, 2020
    Assignee: OLYMPUS CORPORATION
    Inventors: Sumio Kawai, Wataru Hashimoto
  • Patent number: 10748278
    Abstract: An organism evaluation system for analyzing organisms within a fluid flow, comprising one or more of a stimulation section comprising a means for inducing a motive response in a living organism within the fluid flow passing through the stimulation section and a shepherding section comprising a means for temporarily separating such an organism from and then returning it to the fluid flow, a flow normalizing section in fluid communication with the stimulation section and/or shepherding section, and a viewing section in fluid communication with the flow normalizing section, the viewing section comprising a body having formed therein a body cavity defining a viewing port, the viewing section further comprising an optical system mounted relative to the body for viewing the fluid flow within the viewing port through a cavity first opening, whereby image data relating to the fluid flow and organisms therein is acquired via the optical system for analysis.
    Type: Grant
    Filed: May 8, 2017
    Date of Patent: August 18, 2020
    Assignee: SoBru Solutions, Inc.
    Inventor: John Miles Brubacher
  • Patent number: 10738270
    Abstract: Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device.
    Type: Grant
    Filed: March 13, 2020
    Date of Patent: August 11, 2020
    Assignee: The University of British Columbia
    Inventors: Carl L. G. Hansen, Veronique Lecault, James M. Piret, Anupam Singhal
  • Patent number: 10704017
    Abstract: A cell culture carrier module and a cell culture system having the same are provided. A cell tank and a culture medium module respectively communicate with the carrier module. The carrier module includes a reactor, a first fixer, a second fixer and a plurality of cell culture carriers. The reactor has a chamber and at least one inlet/outlet. The inlet/outlet communicates with the chamber. The first fixer is fixed to the reactor and located in the chamber. The second fixer is disposed in the chamber and is movable relative to the first fixer. Two ends of each cell culture carrier are fixed to the first fixer and the second fixer, respectively. The cell culture carriers are in an untwisted state or a twisted state according to a variation in a distance between the first fixer and the second fixer due to a movement of the second fixer.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: July 7, 2020
    Assignee: Industrial Technology Research Institute
    Inventors: Ying-Wen Shen, Ing-Kae Wang, Chia-Jung Lu, Yea-Tzy Deng, Yu-Bing Liou, Sing-Ying Hsieh, Hsin-Hsin Shen, Hsiu-Ying Wang
  • Patent number: 10661275
    Abstract: Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: May 26, 2020
    Assignee: EMULATE, INC.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Joshua Gomes, Kyung Jin Jang
  • Patent number: 10662585
    Abstract: It is an object of the present invention to provide a method for producing ultrafine fibrous cellulose, which is capable of efficiently obtaining ultrafine fibrous cellulose having phosphoric acid groups with a high yield. The present invention relates to a method for producing fibrous cellulose having a fiber width of 1000 nm or less, comprising: a (A) of introducing phosphoric acid groups into cellulose fibers to form crosslinked structures via the phosphoric acid groups, thereby obtaining crosslinked phosphorylated cellulose fibers, a (B) of breaking some or all of the crosslinked structures to obtain crosslink-broken phosphorylated cellulose fibers, and a (C) of performing a mechanical treatment on the crosslink-broken phosphorylated cellulose fibers to obtain fibrous cellulose having a fiber width of 1000 nm or less, wherein, in the (A), crosslinked structures in an amount of 0.05 mmol/g or more and 2.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: May 26, 2020
    Assignee: OJI HOLDINGS CORPORATION
    Inventors: Yuichi Noguchi, Ikue Homma, Yusuke Matsubara
  • Patent number: 10626435
    Abstract: Nanofluidic devices and methods of the invention are capable of autonomously isolating individual microbial cells using constrictive channels and growing monocultures of the cells for automated characterization of their biochemical properties and interactions with mammalian cells. Single microbial cells, such as bacterial cells, are isolated directly from environmental sources and cultured using chemical factors from their native environment.
    Type: Grant
    Filed: March 27, 2013
    Date of Patent: April 21, 2020
    Assignee: Northeastern University
    Inventors: Edgar Daniel Goluch, Yoshiteru Aoi, Slava Epstein
  • Patent number: 10570360
    Abstract: A method of testing an analyte can include using a cell culture device that includes: at least three distinct chambers between the top wall and bottom wall. The chambers can include: an internal chamber defined by at least one porous internal wall; one or more boundary layer chambers having at least an inner boundary layer chamber defined by the at least one porous internal wall and at least one porous inner boundary layer wall, the at least one porous internal wall having a plurality of pores fluidically coupling the central internal chamber to the one or more boundary layer chamber; and an outer chamber.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: February 25, 2020
    Assignee: CFD RESEARCH CORPORATION
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant
  • Patent number: 10457906
    Abstract: An apparatus to prevent bio-filming during the delivery of photonic energy to a culture or set of organisms in a fluid culture medium, the device including a culture medium, a container configured to house the culture medium, and an assembly to generate and deliver a liquid energy transfer medium between the culture medium and the photonic device, the assembly including one or more apertures sized and shaped to form one or more liquid replenishing lenses and a fluid-light unit in the container to house the photonic device and the liquid energy transfer medium, the photonic device is at least one from among a photoreceptor, a photoemitter, or a combination of the photoreceptor and the photoemitter, and further including a pump coupled to the fluid-light unit, the assembly is configured to convey the photonic energy between the photonic device and the culture medium through the liquid energy transfer medium.
    Type: Grant
    Filed: April 14, 2015
    Date of Patent: October 29, 2019
    Assignee: Staterra LLC
    Inventors: Jared P. Green, Eric R. Brooks
  • Patent number: 10435658
    Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: October 8, 2019
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones
  • Patent number: 10435663
    Abstract: This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: October 8, 2019
    Assignee: ABEC, INC.
    Inventor: Cameron Knight
  • Patent number: 10427155
    Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: October 1, 2019
    Assignee: Illumina, Inc.
    Inventors: Alex Aravanis, Boyan Boyanov, M. Shane Bowen, Dale Buermann, Alexander Hsiao, Behnam Javanmardi, Tarun Khurana, Poorya Sabounchi, Hai Quang Tran
  • Patent number: 10364410
    Abstract: One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: July 30, 2019
    Assignee: Massachusetts Institute of Technology
    Inventor: Andreas Mershin
  • Patent number: 10330585
    Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: June 25, 2019
    Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
    Inventors: Osamu Koseki, Ryo Suenaga, Masahiro Kuninori
  • Patent number: 10273441
    Abstract: The invention relates to a perfusion manifold assembly that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with an assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. The invention further relates to a drop-to-drop connection scheme for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: April 30, 2019
    Assignee: EMULATE, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Guy Robert Thompson, II, Petrus Wilhelmus Martinus van Ruijven, Matthew Daniel Solomon, Christian Alexander Potzner, Patrick Sean Tuohy, Joshua Gomes, Norman Wen, Jacob Freake, Doug Sabin
  • Patent number: 10233416
    Abstract: A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: March 19, 2019
    Assignee: EMULATE, INC.
    Inventors: Christopher David Hinojosa, Guy Robert Thompson, II, Joshua Gomes, Jacob Freake, Doug Sabin
  • Patent number: 10184102
    Abstract: A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: January 22, 2019
    Assignee: Emulate, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Joshua Gomes, Jose Fernandez-Alcon
  • Patent number: 10179901
    Abstract: The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: January 15, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Clark K. Colton, Jeffrey R. Millman
  • Patent number: 10175140
    Abstract: The present invention provides a portable luminescence detector combined with a mobile device, which mainly comprises a body, a focusing lens module, a light source module, a sample tray, and an image capturing module, wherein the image capturing module can be a lens of the mobile device, the body can be disassembled into multiple parts to act as holders for the portable luminescence detector. When the portable luminescence detector of the present invention is used in conjunction with the mobile device, the sample tray is placed at a position corresponding to that of the focusing lens module, and the light source module is used for providing a light source band, then the image capturing module works with the focus lens module to capture an image of the sample under the light source band and to provide the image for RGB spectral analysis or real-time image RGB spectral analysis.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: January 8, 2019
    Assignee: SOOCHOW UNIVERSITY
    Inventors: Mei-Lin Ho, Chun-Yen Lin, Wei-Ren Lin, Ying-Chu Wang, Kuen-Yuh Chung
  • Patent number: 10125342
    Abstract: Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: November 13, 2018
    Assignee: EMULATE, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Guy Robert Thompson, II, Petrus Wilhelmus Martinus van Ruijven, Matthew Daniel Solomon, Christian Alexander Potzner, Patrick Sean Tuohy
  • Patent number: 10093953
    Abstract: A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: October 9, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Vijayanand Suryakant Moholkar
  • Patent number: 10093540
    Abstract: According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the liquid hydrocarbon with the renewable hydrogen or alternatively, the renewable hydrogen can be added to a reactor operated so as to simultaneously desulfurize and hydrogenate the hydrocarbons. The present invention enables a party to receive a renewable fuel credit for the transportation or heating fuel.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: October 9, 2018
    Assignee: Iogen Corporation
    Inventor: Brian Foody
  • Patent number: 10092906
    Abstract: Disclosed are cartridges and modules that may be utilized in diagnostic systems and methods. The cartridge includes a flexible seal that caps the cartridge. The flexible seal has an opening for a pipette tip, and the flexible seal is configured to create a sealed environment when a pipette tip is positioned in the opening of the flexible seal and when the pipette tip is moved in the X-axis, Y-axis, and Z-axis.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: October 9, 2018
    Assignee: LUMINEX CORPORATION
    Inventors: Tom Westberg, Bruce Richardson, Ryan Griswold, Chris Lewis