Bioreactor Patents (Class 435/289.1)
  • Patent number: 10435658
    Abstract: A fluid mixing system includes a support housing having an interior surface bounding a chamber. A flexible bag is disposed within the chamber of the support housing, the flexible bag having an interior surface bounding a compartment. An impeller is disposed within the chamber of the flexible bag. A drive shaft is coupled with the impeller such that rotation of the drive shaft facilitates rotation of the impeller. A drive motor assembly is coupled with the draft shaft and is adapted to rotate the drive shaft. An adjustable arm assembly is coupled with the drive motor assembly and is adapted to move the drive motor assembly which in turn moves the position of the drive shaft and impeller. An electrical controller can control movement of the adjustable arm.
    Type: Grant
    Filed: October 24, 2012
    Date of Patent: October 8, 2019
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones
  • Patent number: 10435663
    Abstract: This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.
    Type: Grant
    Filed: December 8, 2016
    Date of Patent: October 8, 2019
    Assignee: ABEC, INC.
    Inventor: Cameron Knight
  • Patent number: 10427155
    Abstract: Systems and methods for conducting designated reactions utilizing a base instrument and a removable cartridge. The removable cartridge includes a fluidic network that receives and fluidically directs a biological sample to conduct the designated reactions. The removable cartridge also includes a flow-control valve that is operably coupled to the fluidic network and is movable relative to the fluidic network to control flow of the biological sample therethrough. The removable cartridge is configured to separably engage a base instrument. The base instrument includes a valve actuator that engages the flow-control valve of the removable cartridge. A detection assembly held by at least one of the removable cartridge or the base instrument may be used to detect the designated reactions.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: October 1, 2019
    Assignee: Illumina, Inc.
    Inventors: Alex Aravanis, Boyan Boyanov, M. Shane Bowen, Dale Buermann, Alexander Hsiao, Behnam Javanmardi, Tarun Khurana, Poorya Sabounchi, Hai Quang Tran
  • Patent number: 10364410
    Abstract: One or more light sources may apply stimuli to a colony of organisms. The stimuli may include visible and non-visible light. The stimuli, taken together, may tend to favor survival of organisms that are adapted to perform photosynthesis which involves absorbing energy from infrared or ultraviolet light. In some cases, the set of stimuli may include illuminating the entire colony of organisms with green light, illuminating only a first portion of the colony with pulsed ultraviolet light, and illuminating only a second portion of the colony with pulsed infrared light.
    Type: Grant
    Filed: April 10, 2017
    Date of Patent: July 30, 2019
    Assignee: Massachusetts Institute of Technology
    Inventor: Andreas Mershin
  • Patent number: 10330585
    Abstract: A counting device counts the number of counting objects in liquid in a flexible container. The counting device includes a mounting table on which the container is mounted and a pressing table arranged opposite to a container-arranged side of the mounting table. The mounting table and the pressing table move relatively in a direction of sandwiching the container, and a light-passing hole facing a surface of the container is provided in at least one of the mounting table and the pressing table. The pressing table includes one or more thickness-setting members that sandwich the container between the pressing table and the mounting table in a periphery of the light-passing hole and on the side opposite to the container in a protruding manner.
    Type: Grant
    Filed: December 21, 2015
    Date of Patent: June 25, 2019
    Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
    Inventors: Osamu Koseki, Ryo Suenaga, Masahiro Kuninori
  • Patent number: 10273441
    Abstract: The invention relates to a perfusion manifold assembly that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with an assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. The invention further relates to a drop-to-drop connection scheme for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: April 30, 2019
    Assignee: EMULATE, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Guy Robert Thompson, II, Petrus Wilhelmus Martinus van Ruijven, Matthew Daniel Solomon, Christian Alexander Potzner, Patrick Sean Tuohy, Joshua Gomes, Norman Wen, Jacob Freake, Doug Sabin
  • Patent number: 10233416
    Abstract: A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate. A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: March 19, 2019
    Assignee: EMULATE, INC.
    Inventors: Christopher David Hinojosa, Guy Robert Thompson, II, Joshua Gomes, Jacob Freake, Doug Sabin
  • Patent number: 10184102
    Abstract: A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: January 22, 2019
    Assignee: Emulate, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Joshua Gomes, Jose Fernandez-Alcon
  • Patent number: 10179901
    Abstract: The invention provides methods and compositions relating to differentiated cell populations that derive from pluripotent stem cells. The methods relate to reducing the number of residual stem cells present in such populations. The compositions include differentiated cell populations that contain reduced number of stem cells or that contain no stem cells. Pluripotent stem cells may be reduced in number and/or function through exposure to low oxygen levels.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: January 15, 2019
    Assignee: Massachusetts Institute of Technology
    Inventors: Clark K. Colton, Jeffrey R. Millman
  • Patent number: 10175140
    Abstract: The present invention provides a portable luminescence detector combined with a mobile device, which mainly comprises a body, a focusing lens module, a light source module, a sample tray, and an image capturing module, wherein the image capturing module can be a lens of the mobile device, the body can be disassembled into multiple parts to act as holders for the portable luminescence detector. When the portable luminescence detector of the present invention is used in conjunction with the mobile device, the sample tray is placed at a position corresponding to that of the focusing lens module, and the light source module is used for providing a light source band, then the image capturing module works with the focus lens module to capture an image of the sample under the light source band and to provide the image for RGB spectral analysis or real-time image RGB spectral analysis.
    Type: Grant
    Filed: November 1, 2017
    Date of Patent: January 8, 2019
    Assignee: SOOCHOW UNIVERSITY
    Inventors: Mei-Lin Ho, Chun-Yen Lin, Wei-Ren Lin, Ying-Chu Wang, Kuen-Yuh Chung
  • Patent number: 10125342
    Abstract: Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: November 13, 2018
    Assignee: EMULATE, Inc.
    Inventors: Daniel Levner, Josiah Daniel Sliz, Christopher David Hinojosa, Guy Robert Thompson, II, Petrus Wilhelmus Martinus van Ruijven, Matthew Daniel Solomon, Christian Alexander Potzner, Patrick Sean Tuohy
  • Patent number: 10092906
    Abstract: Disclosed are cartridges and modules that may be utilized in diagnostic systems and methods. The cartridge includes a flexible seal that caps the cartridge. The flexible seal has an opening for a pipette tip, and the flexible seal is configured to create a sealed environment when a pipette tip is positioned in the opening of the flexible seal and when the pipette tip is moved in the X-axis, Y-axis, and Z-axis.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: October 9, 2018
    Assignee: LUMINEX CORPORATION
    Inventors: Tom Westberg, Bruce Richardson, Ryan Griswold, Chris Lewis
  • Patent number: 10093953
    Abstract: A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: October 9, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Vijayanand Suryakant Moholkar
  • Patent number: 10093540
    Abstract: According to the present invention, organic material is converted to biogas through anaerobic digestion and the biogas is purified to yield a combustible fluid feedstock comprising methane. A fuel production facility utilizes or arranges to utilize combustible fluid feedstock to generate renewable hydrogen that is used to hydrogenate crude oil derived hydrocarbons in a process to make transportation or heating fuel. The renewable hydrogen is combined with crude oil derived hydrocarbons that have been desulfurized under conditions to hydrogenate the liquid hydrocarbon with the renewable hydrogen or alternatively, the renewable hydrogen can be added to a reactor operated so as to simultaneously desulfurize and hydrogenate the hydrocarbons. The present invention enables a party to receive a renewable fuel credit for the transportation or heating fuel.
    Type: Grant
    Filed: April 24, 2015
    Date of Patent: October 9, 2018
    Assignee: Iogen Corporation
    Inventor: Brian Foody
  • Patent number: 10088398
    Abstract: Fluid containment pressure vessel that eliminates the outer support housing present in certain conventional vessels, and provides an improved cap-to-body interface. In certain embodiments, the cap is threaded and configured to threadingly engage with the body of the pressure vessel, and has sufficient structural integrity to withstand the pressures in the device without the need for a support housing. In certain embodiments, an interlock mechanism is provided that prevents the cap from being opened (e.g., removed from the body) while the device is under pressure. In certain embodiments, a pressure relief valve is provided with a pre-loaded biasing mechanism to achieve the required pressure release rate. The vessel can be used for sample preparation, including purification or concentration of samples, particularly protein samples.
    Type: Grant
    Filed: January 13, 2016
    Date of Patent: October 2, 2018
    Assignee: EMD Millipore Corporation
    Inventors: Phillip Clark, Kurt Greenizen, John Doyle
  • Patent number: 10087414
    Abstract: A continuous device for culturing mammalian cells in a three-dimensional structure for the transplantation or implantation in vivo is described. The culturing device comprises (a) a scaffold formed by a matrix of interconnected growth surfaces spaced at regular intervals and (b) a fluid distribution means at the inlet and the exit of the growth areas. The device is particularly useful for culturing bone cells for dental implants or bone reconstruction.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: October 2, 2018
    Assignee: VIVABIOCELL, S.p.A.
    Inventors: Eric Berry, Francesco Curcio
  • Patent number: 10066198
    Abstract: A device for in-vitro modelling in-vivo tissues of organs that includes a first body portion with at least one access chamber, a second body portion with at least one culturing chamber, and a culturing membrane dividing the at least one access chamber from the culturing chamber. The device further includes a third body portion with at least one actuation chamber having at least one limitation cavity, and an actuation membrane dividing the at least one culturing chamber from the at least one actuation chamber. With the device, a robust actuation system can be provided that does not depend on the mechanical properties of the actuation membrane material, nor on the pressure, and that allows to mimic three-dimensional deformations of the tissue, in particular of lung alveoli.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: September 4, 2018
    Assignee: Universität Bern
    Inventors: Olivier Thierry Guenat, Marcel Felder, Andreas Stucki, Janick Daniel Stucki, Thomas Geiser
  • Patent number: 10030254
    Abstract: The present invention provides a method of producing hydrogen from a waste material, comprising steps of fermenting 100 a fermentation mixture comprising the waste material in a reactor 1 with a headspace 1a under anaerobic conditions, removing 200 hydrogen from a gas from the headspace 1a during fermentation to produce a hydrogen gas and a remainder gas and recirculating 300 at least a portion of the remainder gas back to the headspace 1a. An apparatus for producing hydrogen and recirculating at least a portion of the remainder gas to the headspace 1a is also provided.
    Type: Grant
    Filed: September 2, 2015
    Date of Patent: July 24, 2018
    Assignee: Drexel University
    Inventor: Charles Nathan Haas
  • Patent number: 10022720
    Abstract: A fluidic device for a cartridge for testing biological samples is disclosed herein. In an embodiment, the fluidic device includes a fluidic chamber, at least one microfluidic channel in fluid communication with the fluidic chamber, a venting port configured to apply a pneumatic force to the fluidic chamber, at least one passive valve located within the at least one microfluidic channel and configured to allow or stop fluid flow through the at least one microfluidic channel based on a pressure difference, and a controller configured to control the pneumatic force applied to the fluidic chamber via the venting port.
    Type: Grant
    Filed: June 8, 2016
    Date of Patent: July 17, 2018
    Assignee: CYTOCHIP INC.
    Inventors: Wendian Shi, Yuzhe Ding
  • Patent number: 10004185
    Abstract: A closed loop compost tea brewing system with a hydroponic reservoir and a compost tea brewing assembly configured to brew compost tea. The compost tea brewer assembly includes a tea brewing reservoir. The system includes has a closed loop arrangement fluidly connecting the tea brewing reservoir and the hydroponic reservoir. The closed loop arrangement is configured to provide continuous fluid flow from the tea brewing reservoir to the hydroponic reservoir, which is recirculated back from the hydroponic reservoir to the tea brewing reservoir.
    Type: Grant
    Filed: October 21, 2015
    Date of Patent: June 26, 2018
    Inventor: Matthew Fingerle
  • Patent number: 10005997
    Abstract: The present invention relates to a port installed in a closed-ended photochemical reactor, and more particularly, to a multi-functional port for microalgae cultivation and harvesting that supplies gas for cultivation for culturing microalgae by connecting to the closed-ended photochemical reactor; supplies gas for prevention of precipitation for lifting microalgae during cultivation; and assembles by taking samples of microalgae or selectively combining a plurality of valves which can harvest microalgae, whose cultivation is completed.
    Type: Grant
    Filed: June 1, 2016
    Date of Patent: June 26, 2018
    Assignee: KOREA DISTRICT HEATING CORP.
    Inventors: Won Seok Chang, Deog Yong Ahn, Sang Ho Shin, Ji Hye Yu, Young Jae Lee, Won Kwon Lee, Kwang Keun Choi, Sung Min Kim
  • Patent number: 10006002
    Abstract: A continuous device for culturing mammalian cells in a three-dimensional structure for the transplantation or implantation in vivo is described. The culturing device comprises (a) a scaffold formed by a matrix of interconnected growth surfaces spaced at regular intervals and (b) a fluid distribution means at the inlet and the exit of the growth areas. The device is particularly useful for culturing bone cells for dental implants or bone reconstruction.
    Type: Grant
    Filed: December 7, 2015
    Date of Patent: June 26, 2018
    Assignee: VIVABIOCELL S.P.A.
    Inventor: Francesco Curcio
  • Patent number: 9988651
    Abstract: A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: June 5, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Maxim A. Promtov, Vijayanand Suryakant Moholkar
  • Patent number: 9950015
    Abstract: A portable apparatus for collection and processing of human biological material containing adipose, such as extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with an internal containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. The apparatus has fluid suction features including a suction port and a conduit providing fluid communication from the suction port to a location within the filtrate volume within a tapered portion of the internal containment volume, and optionally including a second suction port and a second conduit to a different location in the filtrate volume with the second conduit being configured to permit elevation adjustment within the filtrate volume.
    Type: Grant
    Filed: September 23, 2015
    Date of Patent: April 24, 2018
    Assignee: The GID Group, Inc.
    Inventors: William W. Cimino, Adam J. Katz, Ramon Llull
  • Patent number: 9944964
    Abstract: A process for increasing alcohol yield from biomass (the form or agro- or forest residue, grains, hops, etc.), involving multiple hydrodynamic cavitation treatments of biomass filtrate—both before and after fermentation. Carbohydrates extracted from biomass are subjected to a first cavitation treatment to promote additional conversion into carbohydrates. The carbohydrates are then combined with bacterial species and nutrients, and allowed to ferment. The fermentation product is subjected to a second hydrodynamic cavitation treatment to promote further conversion of carbohydrates into bioalcohol. After distillation, the bioalcohol is subjected to a second hydrodynamic cavitation treatment to increase its purity.
    Type: Grant
    Filed: February 13, 2017
    Date of Patent: April 17, 2018
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Maxim A. Promtov, Vijayanand Suryakant Moholkar
  • Patent number: 9938493
    Abstract: A method for producing a single-use cell culture container at least partially made of polymer material, wherein the cell culture container is treated with gamma radiation for sterilization, characterized in that the cell culture container is treated with inert gas and/or a defined amount of oxygen before the irradiation in order to prevent the occurrence of an “extended lag phase”.
    Type: Grant
    Filed: May 23, 2013
    Date of Patent: April 10, 2018
    Assignee: SARTORUIS STEDIM BIOTECH GMBH
    Inventors: Wolfgang Ludwig Erl, Joseph Seidl
  • Patent number: 9932562
    Abstract: The invention provides a method of increasing product yield per culture in a population of product-secreting cells bound to a scaffold, for example, a microcarrier, at least partially immersed in a culture medium in a bioreactor. The method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration while leaving the scaffold with the bound cells in the bioreactor; re-feeding the cells by adding a fresh culture medium to increase the culture medium in the bioreactor to approximately the original volume; agitating the culture medium to allow the cells to grow and release a second-secreted product concentration; harvesting product by removing the culture medium with the second-secreted product concentration while leaving the scaffold with the bound cells behind. Also disclosed is a method of increasing virus yield per culture in a population of virus-infected cells bound to a microcarrier suspended in a culture medium.
    Type: Grant
    Filed: October 15, 2013
    Date of Patent: April 3, 2018
    Assignee: GE HEALTHCARE BIO-SCIENCES CORP.
    Inventors: Patrick M. Guertin, Joseph D. Crowell
  • Patent number: 9932550
    Abstract: A cell culture device can include: at least 3 distinct chambers between the top wall and bottom wall. The perimeter walls can include: an internal chamber defined by at least one porous internal wall; one or more boundary layer chambers having at least an inner boundary layer chamber defined by the at least one porous internal wall and at least one porous inner boundary layer wall, the at least one porous internal wall having a plurality of pores fluidically coupling the central internal chamber to the one or more boundary layer chamber; and an outer chamber.
    Type: Grant
    Filed: November 26, 2013
    Date of Patent: April 3, 2018
    Assignee: CFD RESEARCH CORPORATION
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant
  • Patent number: 9909094
    Abstract: A portable apparatus for collection and processing of human biological material, such as containing adipose extracted during a lipoplasty procedure, is useful for multi-step processing to prepare a concentrated product (e.g., stromal vascular fraction) or a fat graft composition. The apparatus has a container with a containment volume with a tissue retention volume and a filtrate volume separated by a filter and with a tapered portion to a collection volume for collecting concentrate product. The apparatus includes a mixing device disposed at least in part within the tissue retention volume, and wherein the mixing device has a rotatable shaft extending from outside of to inside of the internal containment volume and has at least one mixing member that is disposed in the tissue retention volume with at least a portion disposed in the tapered portion of the internal containment volume and that is connected with the shaft to move through the tissue retention volume when the shaft is rotated.
    Type: Grant
    Filed: August 21, 2015
    Date of Patent: March 6, 2018
    Assignee: The GID Group, Inc.
    Inventors: William W. Cimino, Adam J. Katz, Ramon Llull
  • Patent number: 9873121
    Abstract: A system for connecting a reagent container to a socket. The container has a cap with an outlet comprising a collar having an opening on its side, the collar surrounding a spout with a movable valve. The valve has a tube surrounded by a disk. A plug is centrally arranged within one end of the tube so the outer walls of the spout form-fit with the inner side of the tube and the inner walls of the spout form-fit with the outer side of the plug for closing the spout. The plug is connected to one end of the tube and a socket for accommodating the outlet of the container's cap. The socket comprises at least two movable levers to engage into openings of the collar and a socket gasket on one side for taking up the tube of the valve and tubing on the opposite side to drain liquids.
    Type: Grant
    Filed: April 11, 2016
    Date of Patent: January 23, 2018
    Assignee: STRATEC Biomedical AG
    Inventors: Martin Trump, Thomas Lowien, Matthias Barth
  • Patent number: 9752164
    Abstract: Overlay processes are disclosed for making ethanol that not only increase ethanol conversion but do so in a cost effective manner with a reduction in energy requirements per unit of ethanol production. The processes can provide, if desired, higher organic compound as a co-product with ethanol.
    Type: Grant
    Filed: June 14, 2013
    Date of Patent: September 5, 2017
    Assignee: MICROVI BIOTECH, INC.
    Inventors: Fatemeh Razavi-Shirazi, Ameen Razavi, Norman Louis Balmer
  • Patent number: 9744507
    Abstract: A disposable container, such as a deformable bag, for a fluid, having one or more inlets and one or more outlets and an impeller assembly within the container to cause mixing, dispersing, homogenizing and/or circulation of one or more ingredients contained or added to the container. The region within the container that can contain liquid is funnel shaped, which allows very low fluid level mixing, dispensing while mixing, and a reduction or elimination of vortex formation.
    Type: Grant
    Filed: November 5, 2013
    Date of Patent: August 29, 2017
    Assignee: EMD Millipore Corporation
    Inventors: Martin Morrissey, Brian Pereira
  • Patent number: 9725687
    Abstract: The invention provides integrated Organ-on-Chip microphysiological systems representations of living Organs and support structures for such microphysiological systems.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: August 8, 2017
    Assignees: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, VANDERBILT UNIVERSITY
    Inventors: John P. Wikswo, Philip C. Samson, Frank Emmanuel Block, III, Ronald S. Reiserer, Kevin Kit Parker, John A. McLean, Lisa Joy McCawley, Dmitry Markov, Daniel Levner, Donald E. Ingber, Geraldine A. Hamilton, Josue A. Goss, Robert Cunningham, David E. Cliffel, Jennifer Robin McKenzie, Anthony Bahinski, Christopher David Hinojosa
  • Patent number: 9695372
    Abstract: Provided herein are compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to effect production, expression, or both, of products. The methods and compositions are particularly useful in the petrochemical industry.
    Type: Grant
    Filed: September 10, 2008
    Date of Patent: July 4, 2017
    Assignee: Sapphire Energy, Inc.
    Inventors: Jason Pyle, Alex Aravanis
  • Patent number: 9670448
    Abstract: A bioreactor has a reactor bag (12) with a predominantly flexible bag wall (121) that has a rigid bearing area. A stirrer arrangement (14) is in the bearing area and includes rigid profile ring (16) made of a non-magnetic material and fixed in the bearing area. The stirrer arrangement (14) also includes a shaftless stirrer (18) rotatably engaged with the profile ring (16) and having parts that are permanently magnetic. An electric coil arrangement (20) is outside the reactor bag (12) and can produce a rotating magnetic field that interacts with the permanent-magnetic areas of the stirrer (18) to produce a torque that rotates the stirrer (18) and circulates the contents of the bag (12).
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: June 6, 2017
    Assignee: Sartorius Stedim Biotech GmbH
    Inventors: Stefan Zeuch, Thomas Regen, Gerhard Greller, Oscar-Werner Reif
  • Patent number: 9623414
    Abstract: Individual temperature control in multiple reactions performed simultaneously in a spatial array such as a multi-well plate is achieved by thermoelectric modules with individual control, with each module supplying heat to or drawing heat from a single region within the array, the region containing either a single reaction vessel or a group of reaction vessels.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: April 18, 2017
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Jeff Ceremony, Daniel Y. Chu
  • Patent number: 9617521
    Abstract: Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided.
    Type: Grant
    Filed: October 21, 2014
    Date of Patent: April 11, 2017
    Assignee: HemoShear, LLC
    Inventors: Brian R. Wamhoff, Brett R. Blackman, Robert A. Figler, Daniel G. Gioeli, Michael B. Simmers
  • Patent number: 9611496
    Abstract: A process for extracting carbohydrates from biomass and creating bioalcohol from the extracted carbohydrates. Subjecting the biomass to acid or alkali hydrolysis in a first hydrodynamic cavitation process. Filtering the first cavitated biomass to separate a first filtrate containing extracted carbohydrates. Fermenting the first filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. Subjecting the biomass to enzymatic hydrolysis in a second hydrodynamic cavitation process. Filtering the second cavitated biomass to separate a second filtrate containing extracted carbohydrates. Fermenting the second filtrate to create a bioalcohol and separating the bioalcohol by distillation or similar process. The first and second filtrates may be combined and fermented in a single step.
    Type: Grant
    Filed: December 9, 2013
    Date of Patent: April 4, 2017
    Assignee: Cavitation Technologies, Inc.
    Inventors: Roman Gordon, Igor Gorodnitsky, Vijayanand Suryakant Moholkar
  • Patent number: 9606035
    Abstract: A system for applying mechanical stimulation to a biologic sample includes a first biologic sample chamber having a biologic sample holder therein, a support structure for holding the first biologic sample chamber, and a first actuator that can supply a mechanical load to a biologic sample held by the biologic sample holder. The actuator is configured to move into a first position proximate to the chamber in which the actuator can transmit the load to the biologic sample via a first transmission path that includes the biologic sample holder. A controller is configured to automatically move the first actuator into the first position.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: March 28, 2017
    Assignee: TA Instruments-Waters LLC
    Inventors: Chrysanthi Williams, Troy D. Nickel, Brock N. Jacobites, Harry Malkasian, Aaron M. Owens
  • Patent number: 9587283
    Abstract: A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: March 7, 2017
    Assignee: Adello Biologics
    Inventor: Sarfaraz K. Niazi
  • Patent number: 9580793
    Abstract: Methods are disclosed for forming subwavelength coatings for use in the UV, visible, or infrared part of the electromagnetic spectrum. A first material and a second material are deposited onto a substrate. The first material may include dielectric spheres of subwavelength size that self-assemble on the substrate to form a template or scaffold with subwavelength size voids between the spheres into which the second material is deposited or filled. First and second materials are heated on the substrate at a preselected temperature to form the subwavelength coating.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: February 28, 2017
    Assignee: BATTELLE MEMORIAL INSTITUTE
    Inventors: Kyle J. Alvine, Bruce E. Bernacki
  • Patent number: 9545633
    Abstract: This disclosure describes an improved heat transfer system for use in reaction vessels used in chemical and biological processes. In one embodiment, a heat transfer baffle comprising two sub-assemblies adjoined to one another is provided.
    Type: Grant
    Filed: January 28, 2014
    Date of Patent: January 17, 2017
    Assignee: ABEC, Inc.
    Inventor: Cameron Knight
  • Patent number: 9540606
    Abstract: A method of mixing a fluid includes positioning a flexible bag into the chamber of a support housing, the support housing having a plurality of baffles positioned so that the bag folds around the baffles. A fluid is delivered into a compartment of the flexible bag. A mixing element, such as an impeller, is moved within the compartment of the flexible bag so as to mix the fluid therein.
    Type: Grant
    Filed: December 17, 2013
    Date of Patent: January 10, 2017
    Assignees: Life Technologies Corporation, Baxalta Incorporated, Baxalta GmbH
    Inventors: Kurt T. Kunas, Robert V. Oakley, Fauad F. Hasan, Michael E. Goodwin, Jeremy K. Larsen, Nephi D. Jones
  • Patent number: 9500381
    Abstract: A septum is positioned within a disposable vessel and defines a lower chamber and an upper chamber. The septum includes a plurality of apertures that provide fluid communication between the upper chamber and lower chamber. Compressed gas is introduced in the lower chamber to produce fine bubbles rising up throughout the vessel to produce a mixing and gasification needed for the growth of a biological culture and manufacture of a biological product in a nutrient medium. Adding a binding resin to the upper chamber allows harvesting, separation and purification of biological products in the reactor as a single unit operation.
    Type: Grant
    Filed: September 3, 2011
    Date of Patent: November 22, 2016
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz K. Niazi
  • Patent number: 9462801
    Abstract: An object of the present invention is to provide a method for preserving pancreatic islet, a container for preserving pancreatic islet and a kit for transplanting pancreatic islet in order to effectively preserve the pancreatic islet. The present invention presents; a method for preserving a liquid including pancreatic islet in a container for preserving pancreatic islet, wherein at least a part of the container wall face consists of a film having an oxygen permeation coefficient of 2500 cm3/m2·day·atm or greater; a method for preserving a liquid including pancreatic islet in a container for preserving pancreatic islet, wherein at least a part of the container wall face consists of a film having a carbon dioxide permeation coefficient of 1000 to 20000 cm3/m2·day·atm; and a container for preserving pancreatic islet for use in the above-described method, and a kit for transplanting pancreatic islet comprising the container for preserving pancreatic islet.
    Type: Grant
    Filed: July 3, 2007
    Date of Patent: October 11, 2016
    Assignees: NIPRO CORPORATION, TOHOKU UNIVERSITY
    Inventors: Masafumi Goto, Yoshihiro Yoshikawa, Kei Matsuo
  • Patent number: 9464282
    Abstract: There are provided a radiofrequency device for increasing amount of a bioactive substance in a plant cell and a plant cell culture method for increasing amount of useful intracellular secondary metabolites by using the radiofrequency device. The cell culture method of the present invention makes it possible to increase specific secondary metabolites such as daidzein, equol, and the like in a cell and thus can be used for development into various medicines, agricultural pesticides, spices, pigments, food additives, and cosmetics containing bioactive substances. Further, the cell culture method of the present invention improves conventional cell culture methods limitedly used for specific cells or specific metabolites for increasing amount of intracellular bioactive substances and thus can be widely applied to production of cells and secondary metabolites.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: October 11, 2016
    Assignee: BIO-FD&C CO., LTD.
    Inventors: Sang-Hyun Moh, Jeong-Hun Lee, Hyo-Hyun Seo, Dong-Sun Shin, Moon-Jin Cho, Hyo-Seok Kang, Hae-Soo Jung, Hyun-Jin Oh, Yeong-Wook Yu, Ji-Hong Moh, Mun-Seong Heo, Ser-Jong Yoo, Hyun-E Lee, Jin-Hyeong Lee, Yu-Ri Lee, Eun-Ae Kim, Seung-Jun Lee
  • Patent number: 9453252
    Abstract: An apparatus for assaying a tumor drug delivery vehicle and or drug can include an idealized microvascular network (IMN) of one or more interconnected idealized flow channels in fluid communication through a porous wall with a tissue space (e.g., idealized tissue space) containing animal cells and means for quantifying drug delivery through the IMN to the animal cells.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: September 27, 2016
    Assignee: CFD RESEARCH CORPORATION
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant, Charles Joseph Garson
  • Patent number: 9416429
    Abstract: A method for optimization of a response of a biological system includes: (1) obtaining measurements of a biological system in response to varying concentrations of drugs in a drug combination; (2) fitting the measurements into a model of the biological system; and (3) using the model of the biological system, predicting concentrations of the drugs in the drug combination to yield an optimized response of the biological system.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: August 16, 2016
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Chih-Ming Ho, Pak Kin Wong, Ren Sun, Fuqu Yu
  • Patent number: 9328323
    Abstract: This invention relates generally to systems and methods for digestion of solid waste that simplify solids handling. In certain embodiments, anaerobic methane extraction takes place for a period of time (e.g., from 1 to 4 weeks), after which an aerobic composting process begins in the same chamber. The organic waste remains in place and oxygen (e.g., in air) is forced into the chamber for an additional period of time (e.g., from 2 to 4 weeks). At the conclusion of the aerobic phase, the process yields a rough compost product that is stable and pathogen free. The rough compost can be further processed and blended to create high value engineered soils.
    Type: Grant
    Filed: June 28, 2013
    Date of Patent: May 3, 2016
    Assignee: AIKAN NORTH AMERICA, INC.
    Inventor: Christian Boggild Smed Christensen
  • Patent number: 9322043
    Abstract: The invention provides methods for treatment of feedstock to reduce the relative viscosity and promote release of fermentable sugars.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: April 26, 2016
    Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.
    Inventors: Lonnie O. Ingram, Claudia C. Geddes, James J. Peterson, Michael T. Mullinnix, Keelnatham Shanmugam