Methods Of Sampling Or Inoculating Or Spreading A Sample; Methods Of Physically Isolating An Intact Micro-organism Patents (Class 435/30)
  • Patent number: 10351894
    Abstract: A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ?10 ?m flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region.
    Type: Grant
    Filed: November 20, 2015
    Date of Patent: July 16, 2019
    Inventors: Dino Di Carlo, Soojung C. Hur, Albert J. Mach
  • Patent number: 10323222
    Abstract: The invention relates to a device (1) for automated inoculation of a culture medium, the device being of the type comprising: a clamp (2) suitable for gripping the inoculation tool; a clamp-holder (3); a support base (4) carrying the assembly formed by the clamp (2) and the clamp-holder (3); and a support structure (5) for supporting the support base (4) and for enabling the support base (4) to be moved along three axes.
    Type: Grant
    Filed: May 20, 2014
    Date of Patent: June 18, 2019
    Inventors: Christian Curel, Michel Roch, Jean-Louis Cariou
  • Patent number: 10213143
    Abstract: The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.
    Type: Grant
    Filed: May 25, 2018
    Date of Patent: February 26, 2019
    Assignee: Purdue Research Foundation
    Inventors: Robert Graham Cooks, Zheng Ouyang, Chien-Hsun Chen, Ziqing Lin, Livia Schiavinato Eberlin
  • Patent number: 10208331
    Abstract: A bioparticle collection device and an aerosol collection system. The bioparticle collection device includes a collection medium including a plurality of fibers formed into a fiber mat and configured to collect bioparticles thereon, and includes a viability enhancing material provider disposed in a vicinity of the plurality of fibers and configured to provide a viability enhancing material to the collected bioparticles to maintain viability of the bioparticles collected by the fiber mat. The aerosol collection system includes an aerosol pumping device configured to entrain particles in an gas stream, an aerosol saturation device configured to saturate the particles in the gas stream with a biocompatible liquid, and an aerosol collection medium downstream from the aerosol saturation device and including a plurality of fibers formed into a fiber mat for collection of the saturated aerosol particles.
    Type: Grant
    Filed: April 27, 2018
    Date of Patent: February 19, 2019
    Assignee: Research Triangle Institute
    Inventors: David Samuel Ensor, Howard Jerome Walls, Karin K. Foarde
  • Patent number: 10059975
    Abstract: The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.
    Type: Grant
    Filed: October 30, 2009
    Date of Patent: August 28, 2018
    Assignee: bioMerieux, Inc.
    Inventors: John Walsh, Jones M. Hyman, Thurman Thorpe, Bradford Clay
  • Patent number: 10058306
    Abstract: Methods are disclosed for non-invasively obtaining fetal cells from a pregnant female. The methods include placing an absorbent medium in an interlabial or intravaginal space or adjacent to the perineum at the vaginal opening of the pregnant female, and collecting vaginal fluid comprising cells in the absorbent medium while the absorbent medium is interlabial or intravaginal space or adjacent to the perineum at the vaginal opening. The absorbent medium is removed and cells are isolated from the absorbent medium to obtain the fetal cells. The fetal cells can be, for example, somatic cells, embroyic stem cells, fetal stem cells or trophoblast cells.
    Type: Grant
    Filed: June 24, 2013
    Date of Patent: August 28, 2018
    Assignee: Preprogen, LLC
    Inventors: William H. Fleming, Shalom Z. Hirschman
  • Patent number: 9861663
    Abstract: The present invention provides a composition including a poly-l-lactic acid (PLLA) and polylactic-co-glycolic-acid (PLGA) scaffold on which neuronal tissue is grown ex-vivo. Furthermore, the invention provides a method for making ex-vivo cellular vasculature networks, and a method for treating a neuronal injury in a subject by implanting the current composition.
    Type: Grant
    Filed: February 25, 2013
    Date of Patent: January 9, 2018
    Inventors: Shulamit Levenberg, Jacob Blumenthal
  • Patent number: 9834806
    Abstract: A disposable microbe-collecting carrier cartridge and a carrier treating apparatus which efficiently convert microbes collected on a carrier into a solution and prevent variations in the amount of retrieval of microbes upon concentration on a filter are provided. A cartridge formed of an upper lid having a plurality of through holes, a container in an inverted-cone shape having both of a liquid storage sink unit having a filter at the bottom and an air suction path, and a support base for setting up a thermoplastic carrier, and a carrier treating apparatus formed of a dispensing nozzle for liquid supply, a liquid supply mechanism, a liquid heating mechanism, and a suction pump for filtration are prepared. Warm water is supplied onto the filter set up on a bottom surface of the inverted-cone-shaped. The contact with the warm water causes the carrier to be solated filtered through the filter.
    Type: Grant
    Filed: June 25, 2009
    Date of Patent: December 5, 2017
    Inventors: Hideyuki Noda, Masahiro Okanojo, Kenko Uchida, Norihito Kuno
  • Patent number: 9675973
    Abstract: A specimen delivery apparatus includes a housing having a backplane. The backplane includes at least one fluid communication port. The housing has an open state and a closed state. A midplane has a cavity for holding a sample. The midplane is sealed within the housing when the housing is in the closed state. A first actuator is disposed to move fluid within the housing when the housing is in a closed state and the first bulb is actuated. Various embodiments provide for caching of the fluid as well as staging to permit further preparation of the specimen prior to delivery. Various features roil the fluid to assist in extraction, mixing, and transport of the sample with the fluid. A destructible seal prevents fluid communication through the fluid communication port while the seal is intact. Communication of fluid through the fluid communication port is enabled only when the destructible seal is not intact.
    Type: Grant
    Filed: June 14, 2013
    Date of Patent: June 13, 2017
    Inventors: John C. Carrano, Roland Schneider, John J. Carrano
  • Patent number: 9504739
    Abstract: The subject invention provides materials and methods that effectively support innate immunity and/or disperse pathogenic biofilms using readily available, nontoxic, natural substances, while supporting restoration of normal microbiotic homeostasis. In one embodiment, the subject invention provides anti-biofilm compositions comprising one or more probiotic organisms, anti-microbial honey, and other ingredients such as prebiotic compounds, other hive products, green tea derivatives, other plant derivatives, and vitamin D3.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: November 29, 2016
    Inventors: Eva A. Berkes, Nicholas T. Monsul
  • Patent number: 9506911
    Abstract: The present invention provides a device having an internal and an external functional element for producing a fluid stream having a core stream and an sheath stream. The device having an internal and an external functional element is distinguished by an internal component for shaping a core stream, an external functional element having a section for guiding an sheath stream and a section for focusing the sheath stream having core stream at the free end of the functional element, wherein the device having an internal and an external functional element is formed in such a manner that the functional element for shaping the core stream is spatially separated from the external functional element having a section for focusing the sheath stream and/or the section for guiding the sheath stream. In a further aspect, an apparatus for separating particles having this device having an internal and an external functional element is provided. In particular, this is a flow-cytometry apparatus in this case.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: November 29, 2016
    Assignee: Laser Zentrum Hannover e.V.
    Inventors: Ulrich Klug, Andre Neumeister, Detlefr Rath
  • Patent number: 9470681
    Abstract: Apparatuses for determining whether a substance is carried in a fluid are disclosed. One such apparatus includes a body having: a plurality of spaced-apart capture surfaces configured to contact the fluid and capture the substance from the fluid; and a plurality of fluid guiding surfaces spaced apart by a predetermined distance from respective at least portions of respective ones of the plurality of capture surfaces. Another such apparatus includes: a fluid conduit to direct flow of the fluid; and at least one element removably positioned in the fluid conduit and having at least one capture surface configured to contact the fluid and capture the substance from the fluid.
    Type: Grant
    Filed: January 14, 2011
    Date of Patent: October 18, 2016
    Assignee: The University of British Columbia
    Inventors: Deborah Roberts, Mina Hoorfar, Sina Jomeh, Rony Das
  • Patent number: 9250204
    Abstract: A method for forming a sensor includes forming a channel in substrate, forming a sacrificial layer in the channel, forming a sensor having a first dielectric layer disposed on the substrate, a graphene layer disposed on the first dielectric layer, and a second dielectric layer disposed on the graphene layer, a source region, a drain region, and a gate region, wherein the gate region is disposed on the sacrificial layer removing the sacrificial layer from the channel.
    Type: Grant
    Filed: January 26, 2015
    Date of Patent: February 2, 2016
    Assignee: International Business Machines Corporation
    Inventors: Dechao Guo, Shu-Jen Han, Chung-Hsun Lin, Ning Su
  • Patent number: 9157063
    Abstract: The present invention includes methods and apparatus to separate X or Y-chromosome bearing sperm cells in a population by first placing the population of sperm cells at physiological pH environment, and simultaneously contacting the population of sperm cells with one or more additional sub-environments with different pH values. The exposure allows mobile sperm cells bearing X or Y-chromosome to migrate to the different pH sub-environments, wherein each cell only exposed or come in contact with one pH sub-environment. Finally, the collecting X or Y-chromosome enriched population of sperm cells is performed.
    Type: Grant
    Filed: October 31, 2007
    Date of Patent: October 13, 2015
    Assignee: Texas Tech University System
    Inventors: Samuel D. Prien, Lindsay L. Penrose
  • Patent number: 9157887
    Abstract: A method for forming a sensor includes forming a channel in substrate, forming a sacrificial layer in the channel, forming a sensor having a first dielectric layer disposed on the substrate, a graphene layer disposed on the first dielectric layer, and a second dielectric layer disposed on the graphene layer, a source region, a drain region, and a gate region, wherein the gate region is disposed on the sacrificial layer removing the sacrificial layer from the channel.
    Type: Grant
    Filed: August 14, 2013
    Date of Patent: October 13, 2015
    Assignee: International Business Machines Corporation
    Inventors: Dechao Guo, Shu-Jen Han, Chung-Hsun Lin, Ning Su
  • Patent number: 9139806
    Abstract: A microorganism counting cell of the present invention includes bottomed cylindrical vessel that includes upper surface opening and cylindrical retaining body disposed vertically on a bottom surface in vessel, a collecting element provided on a lower end portion of a rod-shaped cotton swab being inserted in retaining body from upper surface opening. In the microorganism counting cell, elution protrusion is provided on an interior side surface of retaining body, and elution groove that pierces retaining body from an inside to an outside is provided on a side surface of retaining body.
    Type: Grant
    Filed: August 29, 2011
    Date of Patent: September 22, 2015
    Assignee: Panasonic Healthcare Holdings Co., Ltd.
    Inventor: Kazufumi Oouchi
  • Patent number: 9127245
    Abstract: A method of arranging a large number of living bodies, such as cells, embryos, or organisms rapidly, individually, and one by one, a holding sheet for the method, and a device for processing the living bodies. The method of holding living bodies includes using a sheet in which multiple through-holes with a size capable of holding one of the target living bodies, but not capable of holding two or more of the living bodies, are provided, to thereby arrange and hold the living bodies one by one in the multiple through-holes in the sheet together with a liquid.
    Type: Grant
    Filed: November 27, 2008
    Date of Patent: September 8, 2015
    Assignees: Canon Kabushiki Kaisha, Mie University
    Inventors: Takeshi Miyazaki, Kohei Watanabe, Toshio Tanaka, Yasuhito Shimada, Norihiro Nishimura
  • Patent number: 9096883
    Abstract: Methods and systems for detecting the presence of a target microorganism in a liquid sample are provided. Methods comprise the steps of passing the liquid sample through a surface filter, placing the surface filter into contact with a culture device, incubating the culture device for a period of time and detecting the presence of a target microorganism. Methods may be used with an automated detection system.
    Type: Grant
    Filed: December 18, 2008
    Date of Patent: August 4, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Stephen B. Roscoe, Manjiri T. Kshirsagar, Cynthia D. Zook
  • Publication number: 20150148255
    Abstract: This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a plurality of processing vessels and a collector. The collector funnels portions of the target material from the suspension through a cannula and into the processing vessels. Sequential density fractionation is the division of a sample into fractions or of a fraction of a sample into sub-fractions by a step-wise or sequential process, such that each step or sequence results in the collection or separation of a different fraction or sub-fraction from the preceding and successive steps or sequences. In other words, sequential density fractionation provides individual sub-populations of a population or individual sub-sub-populations of a sub-population of a population through a series of steps.
    Type: Application
    Filed: January 30, 2015
    Publication date: May 28, 2015
    Applicant: RareCyte, Inc.
    Inventors: Lance U'ren, Daniel Campton, Joshua Nordberg, Steve Quarre, Jonathan Lundt
  • Patent number: 9034596
    Abstract: The method for fluorescent staining of cellular and intracellular membranes includes contacting a cell sample, such as a cell culture, with a fluorescent probe including Zn(II) meso-tetrakis(N-n-hexylpyridinium-4-yl)porphyrin tetrachloride (ZnTnHex-4-PyPCl4). The fluorescent probe is an amphiphilic, water-soluble compound. The fluorescent probe does not interfere with cell culture media components or other staining products. The fluorescent probe is not harmful to cells.
    Type: Grant
    Filed: April 17, 2014
    Date of Patent: May 19, 2015
    Inventors: Ludmil Tzvetanov Benov, Rima Abdul Ghani Ezzeddine, Anwar Gh. A. Gh. Albanaw, Ines Batinic-Haberle, James D. Craik, Artak Tovmasyan
  • Publication number: 20150132793
    Abstract: A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample.
    Type: Application
    Filed: June 26, 2013
    Publication date: May 14, 2015
    Applicant: Biocartis NV
    Inventors: Roel Penterman, Bart Van Meerbergen
  • Publication number: 20150132746
    Abstract: Provided herein is technology relating to the collection of biological samples and particularly, but not exclusively, to compositions, methods, and uses related to using a biopolymer substrate to collect biological samples for analysis.
    Type: Application
    Filed: May 29, 2013
    Publication date: May 14, 2015
    Inventors: Astrid Gjelstad, Lars Erik Eng Eibak, Anne Bee Hegge, Knut Einar Rasmussen, Stig Pedersen-Bjergaard
  • Publication number: 20150125896
    Abstract: Cell binding peptides are provided for binding to cells including urothelial and thyroid follicular cells. The peptides are useful for detection and diagnosis of cancer including bladder and thyroid cancer. A device and method for using the device for capturing cells is provided, the device includes a support having attached cell binding peptide. The support can be a slide and the device can be used for detection and diagnosis of cancer including bladder and thyroid cancer. A kit is provided with instructions for capturing cells and a support with attached cell binding peptide for detection and diagnosis of bladder and thyroid cancer.
    Type: Application
    Filed: October 24, 2014
    Publication date: May 7, 2015
    Inventors: William David Culp, Martyn Kerry Darby, Dalia Isolda Juzumiene, Magdalena Krajewska, Natalia Lygina, Juhua Morrison, Shrikumar Ambujakshan Nair, William Bourchier Siesser, Danuta Wronska
  • Publication number: 20150125895
    Abstract: Various embodiments disclosed herein provide for reagents and methods for rapidly isolating viable microbial cells, including S. pneumoniae, from positive blood culture samples. The resulting microbial pellet can be used for both identification and growth-based methods such as antimicrobial susceptibility testing. The buffers described herein may contain a base solution, non-ionic detergents, thiols, and optionally, ammonium chloride. The disclosed methods provide a process for rapidly isolating and concentrating viable microorganism (s) from PBC samples using only one sample preparation tube and centrifugation while removing cellular debris from the mammalian blood cells that may interfere with identification methods.
    Type: Application
    Filed: February 28, 2013
    Publication date: May 7, 2015
    Applicant: Becton, Dickinson and Company
    Inventors: Susan M. Kircher, Vanda White, William B. Brasso, Dyan Luper, James Y. Zhou, Julie L. Rosales, Jeffery H. Bruton, John D. Mantlo, Adrien P. Malick, Donald R. Callihan, Ben Turng, Liping Feng, Curtis M. Gosnell, Patrick Shawn Beaty, John P. Douglass
  • Publication number: 20150118704
    Abstract: The introduction of tools to study, control or expand the inner-workings of algae has been slow to develop. Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. The methods of the disclosure have been shown to work in wild-type algae that have an intact cell wall. Developed using Chlamydomonas reinhardtii, this method is also successful with less studied algae, including Neochloris oleoabundans and Scenedesmus dimorphus, thus providing a new and versatile tool for algal research and modification. The method of delivering a cargo compound to an algal cell comprises contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 30, 2015
    Inventors: Erika Geihe, Brian Trantow, Paul Wender, Joel M. Hyman, Bahram Parvin
  • Publication number: 20150111241
    Abstract: An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet.
    Type: Application
    Filed: April 17, 2013
    Publication date: April 23, 2015
    Inventors: Allison Hubel, Jacob Hanna
  • Publication number: 20150111242
    Abstract: The present invention provides an apparatus and method for microdissecting a biological sample. In particular, apparatuses of the invention include a cell collecting device 100 that is operated by a pneumatic device 200. In one particular embodiment, apparatuses of the invention are used for collecting and transferring cell sample to and from cell collecting device 100.
    Type: Application
    Filed: April 25, 2013
    Publication date: April 23, 2015
    Applicant: The Regents of the University of Colorodo, a body corporate
    Inventors: Wilbur Franklin, Aaron Lieberman, Mark Palmer, Willem Berglund, Stephen DeMars, Andrew Hanuszek, Dara Aisner, Qing Ren, Eric Kelso
  • Publication number: 20150111196
    Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.
    Type: Application
    Filed: December 22, 2014
    Publication date: April 23, 2015
    Inventors: Zheng XIA, Yu ZHOU, John LARSEN, Guocheng SHAO, Shane PETERSON, Marjorie FAUST
  • Publication number: 20150104824
    Abstract: A separation device that can include a separation tube or container that has a wall defining an internal volume into which a material can be placed is disclosed. The material can include a multi-component mixture or solution. A secondary tube or withdrawal cannula can be placed and/or moved within the tube to withdrawal a material form the tube.
    Type: Application
    Filed: October 9, 2014
    Publication date: April 16, 2015
    Inventors: Trenton WALKER, Matthew D. Landrigan, Michael D. Leach, Scott R. King, Randel Dorian, Richard W. Storrs
  • Publication number: 20150104825
    Abstract: A cell capture, disruption, and extraction apparatus includes a disruption chamber configured to receive cell solution and having therein a plurality of abrasives, which can include diamond powder, variably and multi dimensionally disbursed therein, and a pestle positioned in the disruption chamber. The apparatus includes an actuation device configured to agitate the disruption chamber and/or pestle, movement of the abrasives tearing cell structure in the solution to access its contents. A binding column or size exclusion column can be positioned downstream of the disruption chamber. The pestle can rotate with respect to the disruption chamber. The pestle can include a nut-shaped core or axle, and/or include a plurality of extrusions. Cell solution can first be introduced in the disruption chamber, the abrasives capturing the cells and allowing therethrough and purging the waste content, then breaking the cell content through the foregoing agitation process.
    Type: Application
    Filed: October 11, 2014
    Publication date: April 16, 2015
    Inventor: Kianoosh Peyvan
  • Publication number: 20150093743
    Abstract: Systems, including apparatus and methods, for the microfluidic manipulation, dispensing, and/or sorting of particles, such as cells and/or beads.
    Type: Application
    Filed: September 5, 2014
    Publication date: April 2, 2015
    Inventors: Amir M. SADRI, Kun GUO, Daniel Y. CHU, Nenad KIRCANSKI, Paul J. PATT, Tal ROSENZWEIG
  • Publication number: 20150093774
    Abstract: There is provided a renewable sensor for sensing or selectively capturing a targeted bacteria in a fluid. The renewable sensor includes a surface, the surface includes a substrate, a biologically or bacterially non-adhesive feature disposed on or in functional proximity to at least a portion of the substrate; and an adhesive elements disposed on or in functional proximity to the non-adhesive feature, a flow channel in operative contact with the surface; and a detector configured to detect the targeted cell type captured on the surface. Also provided are method for using the sensor and the surface.
    Type: Application
    Filed: September 30, 2014
    Publication date: April 2, 2015
    Inventors: Maria Santore, Bing Fang
  • Patent number: 8993235
    Abstract: The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: March 31, 2015
    Inventor: Lee H. Angros
  • Publication number: 20150087010
    Abstract: The present invention provides a filter member. The filter member comprises: a filter for discriminating cells to be analyzed in a sample from other components; a first filter holding member which comprises a first through hole and has a plate-like shape; and a second filter holding member which comprises a second through hole and is fitted into the first filter holding member. When the first and second filter holding members are integrated by fitting the second filter holding member into the first filter holding member, the filter is sandwiched between the first filter holding member and the second filter holding member, and the first through hole is opposed to the second through hole through the filter. A first elastic body is formed on a surface of the first filter holding member, the surface being in contact with the filter. A second elastic body is formed on a surface of the second filter holding member, the surface being in contact with the filter.
    Type: Application
    Filed: September 17, 2014
    Publication date: March 26, 2015
    Inventor: Ryuichiro EBI
  • Patent number: 8986537
    Abstract: Provided are multiple correlations for relationships between MI value for a brightstock extract and the distillation cut point temperature used for separation of the vacuum resid that is used to form the brightstock extract. Based on these correlations, a BSE having a desired MI value can be formed based on an adjustment of the distillation cut point temperature. A first correlation establishes a relationship between a fractional weight boiling temperature for a vacuum resid fraction and a distillation cut point temperature for separating the vacuum resid fraction from at least one distillate fraction in a feedstock. A second correlation establishes a relationship between a fractional weight boiling temperature for a brightstock extract derived from the vacuum resid fraction, and the fractional weight boiling temperature for the vacuum resid fraction. A third correlation has been established between the fractional weight boiling temperature for the brightstock extract and a mutagenicity index value.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: March 24, 2015
    Inventors: Cristina M. Sircar, Keith K. Aldous, James J. Freeman, Katy O. Goyak
  • Patent number: 8986944
    Abstract: The present invention includes methods of enriching rare cells, such as cancer cells, from biological samples, such as blood samples. The methods include performing at least one debulking step on a blood sample and selectively removing at least one type undesirable component from the blood sample to obtain a blood sample that is enriched in a rare cell of interest. In some embodiments magnetic beads coupled to specific binding members are used to selectively removed components.
    Type: Grant
    Filed: August 2, 2006
    Date of Patent: March 24, 2015
    Assignee: Aviva Biosciences Corporation
    Inventors: Douglas T. Yamanishi, Paul G. Hujsak, Sara F. Snyder, George Walker, Junquan Xu, Mingxian Huang, Guoliang Tao, Lei Wu, Xiaobo Wang, Joe Ouyang, Charina Schmitigal, Jing Cheng, Jia Xu
  • Patent number: 8986945
    Abstract: Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.
    Type: Grant
    Filed: August 20, 2007
    Date of Patent: March 24, 2015
    Assignee: Aviva Biosciences Corporation
    Inventors: Ping Lin, Andrea Ghetti, Wenge Shi, Mengjia Tang, Gioulnar I. Harvie, Huimin Tao, Guoliang Tao, Lei Wu, David Cerny, Jia Xu, Douglas T. Yamanishi
  • Publication number: 20150079582
    Abstract: A bismuth-containing concentration agent for microorganisms is provided. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided.
    Type: Application
    Filed: March 4, 2013
    Publication date: March 19, 2015
    Inventors: Manjiri T. Kshirsagar, Evan Koon Lun Yuuji Hajime, Andrew W. Rabins
  • Publication number: 20150079625
    Abstract: The present invention provides a novel category of naphthalene-based two-photon fluorescent probes having a general formula I, wherein: X is selected from the X1, X2, X3 and X4; The mentioned two-photon fluorescent probes have a low fluorescence background in the non-tumor cells and tissues, and have a strong and specific fluorescent signal in the tumor cells and tissues. These probes have a certain level of water-solubility, while having good membrane permeability. In addition, they have a bigger effective two-photon absorption cross section. The compounds of the present invention also have a lower biotoxicity, phototoxicity and photobleaching. There is sufficient difference between the spectral range thereof and that of a biological sample.
    Type: Application
    Filed: March 5, 2012
    Publication date: March 19, 2015
    Inventors: Xiaojun Peng, Hua Zhang, Jiangli Fan, Jingyun Wang
  • Publication number: 20150079614
    Abstract: The present invention provides a liquid sampling, storage, transfer and delivery device comprising housing containing a porous nib. The porous nib in the device contacts the sample, collects the sample, stores the sample, transports the sample inside its porous matrix and releases the sample from the porous matrix upon demand.
    Type: Application
    Filed: November 21, 2014
    Publication date: March 19, 2015
    Inventors: Guoqiang Mao, James P. Wingo
  • Publication number: 20150079588
    Abstract: The present invention relates to methods and devices for observing or studying cells having a cell wall or invertebrate embryos with an oblong eggshell, the devices comprising wells having a conical or frustoconical shape.
    Type: Application
    Filed: March 28, 2013
    Publication date: March 19, 2015
    Inventor: Daniel Riveline
  • Publication number: 20150064740
    Abstract: The present invention is to present a specimen processing apparatus, comprising: an imaging device for imaging a cap of a covered specimen container containing a specimen; an aspirating device including a specimen aspirating tube, moving the specimen aspirating tube so as to pass the specimen aspirating tube through the cap of the covered specimen container and aspirating the specimen contained in the covered specimen container via the specimen aspirating tube; an aspiration controller for controlling a movement of the specimen aspirating tube into the covered specimen container based on an image obtained by the imaging device; and a specimen processing device for processing the specimen aspirated by the aspirating device.
    Type: Application
    Filed: November 11, 2014
    Publication date: March 5, 2015
    Inventors: Takaaki NAGAI, Tokihiro KOSAKA, Yuichi HAMADA
  • Patent number: 8968682
    Abstract: A well plate includes a including a top portion, a bottom portion and a membrane disposed between the top portion and the bottom portion. The top portion defines a sample well in fluid communication with an opening defined by the membrane and in fluid communication with a reservoir defined by the bottom portion. The well plate is configured to be used in a centrifugation process of a test sample including a sample material and a wash liquid. The test sample configured to be received within the sample well and the reservoir. The membrane configured to filter the wash liquid from the test sample during the centrifugation process such that the wash liquid can pass from the reservoir, through the membrane and can be captured within a collection chamber while the sample material remains within the reservoir.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: March 3, 2015
    Assignee: Cytosaver LLC
    Inventor: Matthew Hale
  • Publication number: 20150056611
    Abstract: A method of mixing magnetic particles (3) in a reaction chamber (2) that is part of a microfluidic device and that contains the said particles in suspension, comprises the steps: (a) providing an electromagnetic means (1,1?,6,7) to generate magnetic field sequences having polarity and intensity that vary in time and a magnetic field gradient that covers the whole space of the said reaction chamber (2); (b) applying a first magnetic field sequence to separate or confine the particles (3) so the particles occupy a sub-volume in the volume of the reaction chamber (2); (c) injecting a defined volume of the said reagent in the reaction chamber; and (d) applying a second magnetic field sequence to leads the particles (3) to be homogenously distributed and dynamically moving over a substantial portion of the whole reaction chamber volume.
    Type: Application
    Filed: November 10, 2014
    Publication date: February 26, 2015
    Inventor: Amar Rida
  • Patent number: 8956826
    Abstract: Aspects of the present invention provide novel multi-targeted microbiological screening and monitoring methods having substantial utility for monitoring and control of microbial growth and contaminants, microbiological processes, predictive microbiology, and for exposure and risk assessment. Microbial markers shared by both target and index microbes are used in novel methods for microbial monitoring, monitoring of microbial performance potential, trend analysis, and statistical process control (SPC) in processes or systems that are receptive to a plurality of genetically distinct microbes.
    Type: Grant
    Filed: April 15, 2005
    Date of Patent: February 17, 2015
    Assignee: Institute for Environmental Health, Inc.
    Inventor: Mansour Samadpour
  • Patent number: 8951575
    Abstract: A concentration agent for capture of microorganisms, including diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof, and methods for making the concentration agent.
    Type: Grant
    Filed: September 11, 2013
    Date of Patent: February 10, 2015
    Assignee: 3M Innovative Properties Company
    Inventors: Manjiri T. Kshirsagar, Tushar A. Kshirsagar, Thomas E. Wood
  • Publication number: 20150037833
    Abstract: The present invention relates to a system for collecting and preparing a body fluid sample, the system comprising a sample container (10) comprising a sample cup (38) for receiving the sample, said sample cup comprising graduated indicator markings (14) corresponding to equal increments of sample volume, a removable lid (16) for sealably covering said sample cup, said lid having an access point which is sealed by a septum, and a removable cap (22) which is effective to cover said access point, and a delivery device for containing a plurality of predetermined reagent doses which are to be added to a sample within the sample container in the predetermined doses relative to the volume of the sample.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 5, 2015
    Applicant: Northwestern University
    Inventors: Mark James Fisher, Sally M. McFall, Robert D. Hillman, Zachary J. Walker, Jacqueline Rene Groves, Jennifer Reed, David M. Kelso
  • Publication number: 20150031071
    Abstract: A system and method for distributing and agitating an amount of a liquid over a microscope slide, wherein a distribution plate having a microtextured lower surface and at least one passage extending through the plate is secured at a predetermined distance of 10-250 ?m above the microscope slide. Liquid passes through the passage in the distribution plate, and the distribution plate is transversely reciprocated relative to the microscope slide in the plane of the lower surface of the distribution plate or the plane of the upper surface of the microscope slide.
    Type: Application
    Filed: March 1, 2013
    Publication date: January 29, 2015
    Applicant: Victorious Medical Systems ApS
    Inventor: Lars Winther
  • Publication number: 20150031073
    Abstract: An apparatus and method are described that achieve independent and simultaneous processing of a plurality of substrate-supported biological samples. In one embodiment, substrate holders arranged in a minor arc are independently moveable between a processing position and an access position, and reagents are delivered to substrates held in the substrate holders through a nozzle plate that moves along the arc of substrate holders. The disclosed apparatus and method are particularly suited for implementation of lean processing of biological samples.
    Type: Application
    Filed: October 6, 2014
    Publication date: January 29, 2015
    Inventors: Charles D. Lemme, William Richards, Glen Ward, Austin Ashby, Andrew Ghussen, Lisa Jensen-Long, Kevin Knapp, Rodney Kugizaki, Alain Larson, Paul Richards, Wayne Showalter, Chad Wilkinson
  • Publication number: 20150031072
    Abstract: Sample taking device for extracting a fluid sample from a vessel comprising: a cover portion for closing the vessel such that, in use a headspace is defined between the cover portion and fluid held in the vessel, a sampling chamber comprising a sampling inlet and a sampling outlet, a cannula adapted to provide, in use, fluid communication between the fluid held in the vessel and the sampling chamber through the sampling inlet, and ports to operate the device in at least two operating conditions: a sample preparing condition in which a pressure in the headspace is greater than that of the sampling chamber such that fluid flows through the cannula towards the sampling chamber and can be retained therein, and a sample dispensing condition in which fluid retained in the sampling chamber can be delivered through the sampling outlet and fluid remaining inside the cannula can return to the vessel.
    Type: Application
    Filed: March 7, 2013
    Publication date: January 29, 2015
    Inventors: Andreu Fontova Sosa, Enric SarrĂ´ Casanovas