Involving Viable Micro-organism Patents (Class 435/29)
  • Patent number: 10983120
    Abstract: Provided herein are methods for assessing response to inflammatory disease therapy. The methods include placing a subject on a therapeutic regimen and subsequently performing an immunoassay to generate a score based on quantitative data for expression of biomarkers relating to inflammatory biomarkers. The methods further include recommending that the subject either remains on the therapeutic regimen, or is removed from the therapeutic regimen, based on the score.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: April 20, 2021
    Assignee: Crescendo Bioscience
    Inventor: Eric Sasso
  • Patent number: 10983112
    Abstract: Methods for identification and quantification of bile acids are disclosed. Bile acids in plasma, serum and/or blood such as a dried blood spot are used to identify subjects with a Niemann-Pick disease. The methods include measuring levels of a bile acid, such as 3?,5?,6?-trihydroxycholanic acid, N-(3?,5?,6?-trihydroxy-cholan-24-oyl)glycine, N-(3?,5?,6?-trihydroxy-cholan-24-oyl)taurine, or a combination thereof. Detection of bile acids involve mass spectroscopy and/or a combination of mass spectroscopy and liquid chromatography such as a LC-MS/MS assay. The methods can be used with sphingomyelinase assays to detect, diagnose and differentiate between Niemann-Pick A/B and Niemann-Pick C (NPC) disease.
    Type: Grant
    Filed: April 28, 2017
    Date of Patent: April 20, 2021
    Assignee: Washington University
    Inventors: Daniel Ory, Xuntian Jiang
  • Patent number: 10975429
    Abstract: A system may include a flow cell through which a plurality of reagents may be pumped during a genetic sequencing operation, an effluent line that, in operation, is to conduct a used reagent, a used reagent valve to receive the used reagent from the effluent line and controllable to select one of a plurality of disposal paths for the used reagent, and control circuitry coupled to the used reagent valve that, in operation, is to control the used reagent valve to select a desired one of the disposal paths depending upon which reagent is being pumped though the flow cell.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: April 13, 2021
    Assignee: ILLUMINA, INC.
    Inventors: Bradley Kent Drews, Kevin James Cappa, Matthew William Hage
  • Patent number: 10976307
    Abstract: The disclosure provides methods for analysis of disease cell response to a therapeutic agent. In embodiments, a method comprises administering the therapeutic agent to a disease cell sample from the subject in a device that measures at least one physiological parameter of a cell; determining whether a change occurs in the physiologic parameter of the disease cell sample in response to the therapeutic agent as compared to a baseline measurement or the physiological parameter before administration of the therapeutic agent, and selecting the therapeutic agent that results in the change in the at least one physiologic parameter. In embodiments, the disease cells are whole, viable, and/or label free.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: April 13, 2021
    Assignee: Celcuity Inc.
    Inventors: Lance Gavin Laing, Brian Francis Sullivan
  • Patent number: 10961508
    Abstract: This invention provides a method for stably producing alveolar epithelial progenitor cells from pluripotent stem cells, including steps of culturing pluripotent stem cells in (1) a medium containing activin A and a GSK3? inhibitor, (2) a medium containing a BMP inhibitor and a TGF? inhibitor, and (3) a medium containing BMP4, retinoic acid, and a GSK3? inhibitor.
    Type: Grant
    Filed: April 14, 2014
    Date of Patent: March 30, 2021
    Assignee: KYOTO UNIVERSITY
    Inventors: Kenji Osafune, Shimpei Gotoh, Isao Ito, Michiaki Mishima
  • Patent number: 10954490
    Abstract: Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation.
    Type: Grant
    Filed: July 6, 2018
    Date of Patent: March 23, 2021
    Assignees: The Brigham and Women's Hospital, Inc., Massachusetts Institute of Technology
    Inventors: Jeffrey Michael Karp, Xiaolei Yin, Marc David Succi, Robert Samuel Langer
  • Patent number: 10954222
    Abstract: It has been found that efficient screening for a compound having anticancer activity can be achieved by selecting a compound having activity to induce alternative autophagy using, as an index, formation of fluorescent bright spots due to aggregation of a lysosomal protein to which a fluorescent protein is attached in cells expressing the lysosomal protein.
    Type: Grant
    Filed: February 7, 2013
    Date of Patent: March 23, 2021
    Assignee: National University Corporation Tokyo Medical and Dental University
    Inventors: Shigeomi Shimizu, Takamitsu Hosoya, Michiko Murohashi, Suguru Yoshida
  • Patent number: 10955409
    Abstract: The present invention provides methods of identifying and distinguishing different types of nerve cells in ex vivo cell culture, the method comprising the steps of: a) culturing somato-sensory nerve cells ex vivo, b) loading the nerve cells with a calcium, sodium, or voltage-sensitive indicator or expressing a genetically encoded calcium, sodium, or voltage-sensitive indicator, c) pulsing the nerve cells with an electrical train of bipolar square waves at two different voltages and two different frequencies; wherein the first voltage is 10 V/cm or less (low voltage) and the second voltage is between 12 and 20 V/cm (high voltage); and wherein the first frequency is 5 Hz or less (low frequency) and the second frequency is between 15 and 20 Hz (high frequency), and d) detecting activation of the nerve cell by measuring the changes in the signal intensity of the indicator, wherein low voltage and low frequency activation indicates a first type of cell and activation detected only at high voltage indicates a secon
    Type: Grant
    Filed: April 19, 2018
    Date of Patent: March 23, 2021
    Assignee: AnaBios Corporation
    Inventors: Andrea Ghetti, Yannick Miron, Claudio Ghetti, Paul Miller
  • Patent number: 10945428
    Abstract: This invention relates to a preservative solution and a method to preserve whole blood for cellular and molecular analysis.
    Type: Grant
    Filed: March 3, 2017
    Date of Patent: March 16, 2021
    Assignee: Menarini Silicon Biosystems S.p.A.
    Inventors: Mark Connelly, Chandra Galla Rao, Denis Smirnov
  • Patent number: 10947577
    Abstract: A biological sterilization indicator device is provided. The device comprises a body, a plurality of test microorganisms, and an oxygen-modulated first fluorescent sensor. The body comprises a first layer attached to a second layer, forming at least one isolatable microchamber and at least one primary passageway that provides fluidic communication between ambience and the at least one microchamber. The microchamber has an isolated volume of about 0.5 microliters to about 9.5 microliters. The plurality of test microorganisms and the oxygen-modulated first fluorescent sensor are disposed in the microchamber. A method of using the device to determine the effectiveness of a sterilization process is also provided.
    Type: Grant
    Filed: February 11, 2013
    Date of Patent: March 16, 2021
    Assignee: 3M Innovative Properties Company
    Inventors: Francois Ahimou, Sailaja Chandrapati, Kurt J. Halverson, Heather M. Webb, Michael G. Williams
  • Patent number: 10940477
    Abstract: In accordance with some embodiments, a fluid sample collection and retrieval apparatus including a microfluidic chip, a retrieval tube, a first switch, a second switch and a processor is provided. The microfluidic chip includes a first sample channel, a first fluid directing channel assembly, a first confluence chamber, a first collection channel, a first waste channel, and a retrieval hole. The retrieval hole passes through an outer surface of the microfluidic chip. The retrieval tube is connected to the retrieval hole. The first switch is connected to the microfluidic chip. The second switch is attached to the retrieval tube. The processor is configured to activate the first switch to operate the flow adjustment of the first fluid directing channel assembly and activate the second switch to operate a sample collection in the first collection channel within duration of operating the flow adjustment of the first fluid directing channel assembly.
    Type: Grant
    Filed: July 19, 2018
    Date of Patent: March 9, 2021
    Assignee: MiCareo Taiwan Co., Ltd.
    Inventors: Hui-Min Yu, Jui-Lin Chen, Wei-Feng Fang
  • Patent number: 10935550
    Abstract: This disclosure provides a system for detecting rare cells. The system includes a substrate, an extension coupled to the substrate and extending outwardly from the substrate, and a functionalized graphene oxide disposed on the extension. This disclosure also provides a method for detecting rare cells using the system of this disclosure. The method includes the steps of providing the system and introducing a sample of bodily fluid to the system such that the sample interacts with the functionalized graphene oxide.
    Type: Grant
    Filed: February 3, 2017
    Date of Patent: March 2, 2021
    Assignee: The Regents of the University of Michigan
    Inventors: Sunitha Nagrath, Hyeun Joong Yoon
  • Patent number: 10926257
    Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. Experiments using animal organs show that the digestion device with hydro-mincing capabilities is superior to conventional scalpel mincing and digestion based on recovery of DNA and viable single cells. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities.
    Type: Grant
    Filed: August 28, 2018
    Date of Patent: February 23, 2021
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Jered Haun, Xiaolong Qiu, Elliot Hui, Amrith Karunaratne, Erik Werner
  • Patent number: 10913969
    Abstract: A microfluidic device comprises a substrate transparent for imaging and having a plurality of spatially defined and separated cell channels having a dimension to accommodate cells in monolayer. A respective first end of the cell channels is in fluid connection with a flow input channel having a first end in fluid connection with a first fluid port and a second end in fluid connection with a second fluid port. A respective second end of the cell channels is in fluid connection with a first end of a respective wash channel having a second end in fluid connection with a flow output channel. The flow output channel is in fluid connection with a third fluid port. The wash channels have a dimension too small to accommodate the cells.
    Type: Grant
    Filed: July 19, 2018
    Date of Patent: February 9, 2021
    Assignee: ASTREGO DIAGNOSTICS AB
    Inventors: Johan Elf, Özden Baltekin, Dan I. Andersson
  • Patent number: 10900954
    Abstract: A method using variable bead-oscillation-based stress parameters, including relative bead dimensionality or other structural features as well as stress duration or intensity or overall magnitude, in inducing different shear stresses in biological samples, for testing the effects of different combinations of various defined stress conditions.
    Type: Grant
    Filed: April 13, 2016
    Date of Patent: January 26, 2021
    Assignee: Blaze Medical Devices, Inc.
    Inventors: Kenneth Alfano, Michael Tarasev, Sumita Chakraborty
  • Patent number: 10894093
    Abstract: The present invention relates to therapeutic cells for immunotherapy to treat patients with cancer. In particular, the inventors develop a method of engineering drug-specific hypersensitive T-cell, which can be depleted in vivo by the administration of said specific drug in case of occurrence of a serious adverse even. The invention opens the way to standard and affordable adoptive immunotherapy strategies for treating cancer.
    Type: Grant
    Filed: April 13, 2017
    Date of Patent: January 19, 2021
    Assignee: CELLECTIS
    Inventors: Julien Valton, Veronique Zennou, Philippe Duchateau, Laurent Poirot
  • Patent number: 10889616
    Abstract: The present invention provides one or more compounds of formula (I) for conjugation to small molecules, polymers, peptides, proteins, antibodies, antibody fragments etc.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: January 12, 2021
    Assignee: Tube Pharmaceuticals GmbH
    Inventor: Wolfgang Richter
  • Patent number: 10883936
    Abstract: Provided are methods, for selectively analyzing a cell sample. Viable cells are dyed with a membrane-permeable fluorescent dye, and nonviable cells are dyed with a membrane-impermeable fluorescent quenching dye. The cells are illuminated to cause fluorescent emission from the membrane-permeable fluorescent dye in the viable cells and the membrane-impermeable fluorescent quenching dye in the non-viable cells. The cells are then quenched for at least a portion of fluorescence of the membrane-permeable fluorescent dye in the nonviable cells by the membrane-impermeable fluorescent quenching dye. The cells are then analyzed for viable and nonviable cells.
    Type: Grant
    Filed: October 11, 2019
    Date of Patent: January 5, 2021
    Assignee: SageMedic Corporation
    Inventors: Ekaterina Moroz, Christian Apfel, Kraig K. Anderson
  • Patent number: 10884005
    Abstract: The present invention provides biomarkers useful for evaluating the risk that a subject will develop diabetes, monitoring such risk, identifying members of a population at risk of developing diabetes, calculating risk of a subject developing diabetes, advising subjects of risk for developing diabetes, providing diagnostic tests for identifying subjects at risk for developing diabetes or kits there for, and providing diagnostic tests for determining risk of a subject developing diabetes and kits there for. The present invention also provides compounds and methods for treating subjects.
    Type: Grant
    Filed: October 18, 2016
    Date of Patent: January 5, 2021
    Inventor: Wei Jia
  • Patent number: 10875970
    Abstract: The invention provides a metallopolymer coordination network comprising one or more coinage or similar metals and a glyme or glyme-equivalent. The composition has an amorphous polymer network that is significantly stronger than previously reported supramolecular hydrogels synthesized without glyme. Glyme chain length and water content strongly influence the mechanical, electronic, and optical behavior of the network.
    Type: Grant
    Filed: November 21, 2019
    Date of Patent: December 29, 2020
    Assignee: Colorado State University Research Foundation
    Inventors: William Scott Compel, Christopher J. Ackerson, O. Andrea Wong
  • Patent number: 10870111
    Abstract: A fluidic device includes a plurality of reaction wells, typically in a dense array, with at least one bead retention segment in fluid communication with and spatially separated from at least one signal detection segment. A respective bead retention segment can be configured to hold a single bead, which can have a reagent attached thereto.
    Type: Grant
    Filed: July 19, 2016
    Date of Patent: December 22, 2020
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Michael Ramsey, William Hampton Henley
  • Patent number: 10870733
    Abstract: A process for preparing an algal powder containing a reduced content of proteins, a bioplastic composition formed from such a powder, a process for manufacturing a plastic product obtained from such an algal powder and also the plastic product obtained in this way. Process for preparing an algal powder, especially intended for the manufacture of a plastic product, including the successive steps of: culturing and/or harvesting an algal biomass; reducing by at least 10% the intrinsic amount of proteins of the algae, by weight relative to the weight of proteins of the harvested biomass; drying; and reducing to give powder or granules.
    Type: Grant
    Filed: September 16, 2016
    Date of Patent: December 22, 2020
    Assignee: ERANOVA
    Inventors: Philippe Lavoisier, Ronan Pierre, Maud Benoit
  • Patent number: 10868214
    Abstract: A light emitting diode, a manufacturing method thereof and a display device are provided. The light emitting diode includes a first electrode, an active layer and a second electrode. The active layer is on the first electrode; the second electrode is on a side of the active layer away from the first electrode, and includes a first conductive layer and a second conductive layer sequentially arranged along a direction away from the active layer; the first conductive layer includes a plurality of micropores; and the second conductive layer includes a plurality of conductive nanoparticles.
    Type: Grant
    Filed: August 23, 2018
    Date of Patent: December 15, 2020
    Assignee: BOE TECHNOLOGY GROUP CO., LTD.
    Inventor: Boris Kristal
  • Patent number: 10865416
    Abstract: The present invention is in the field of recombinant biotechnology, in particular in the field of protein expression. The invention generally relates to a method of expressing a protein of interest (POI) from a host cell. The invention relates particularly to improving a host cell's capacity to express and/or secrete a protein of interest and use of the host cell for protein expression. The invention also relates to cell culture technology, and more specifically to culturing cells to produce desired molecules for medical purposes or food products.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: December 15, 2020
    Assignees: Boehringer Ingelheim RCV GmbH & Co KG, Vaitdogen GmbH, Lonza Ltd.
    Inventors: Brigitte Gasser, Diethard Mattanovich, Markus Buchetics
  • Patent number: 10858398
    Abstract: The presently disclosed subject matter is directed to an assay that detects and quantitatively determines the activity of a lytic peptide that exhibits antimicrobial activity, such as LL-37. Particularly, the assay comprises inducing and/or transfecting bacteria to produce high levels of an enzyme, such as ?-galactosidase. The bacteria are then preserved by lyophilization. After a desired amount of time, the bacteria are hydrated with a target sample from a subject suspected of having a specific disease or disorder characterized by an increase in levels of lytic peptide. In the presence of lytic peptide, the enzyme is released from the interior of the bacteria, which can then be detected by alteration of the enzyme substrate. In the absence of lytic peptide, the enzyme remains within the bacteria and no detection of the enzyme occurs.
    Type: Grant
    Filed: April 2, 2019
    Date of Patent: December 8, 2020
    Assignee: Deetex, LLC
    Inventors: James Hartmann, Ryan Landis, Michelle Ryan, Yousseff Mottii
  • Patent number: 10857830
    Abstract: The invention provides a silk fibroin-based anti-counterfeiting mark comprising a silk fibroin film layer and a gold film layer located on the silk fibroin film layer, and the area of the gold film layer is less than that of the silk fibroin film layer. The inventive also provides a method for preparing the silk fibroin-based optical anti-counterfeiting mark, the method comprises preparing an aqueous silk fibroin solution, coating the aqueous silk fibroin solution onto a substrate to obtain a silk fibroin film, and sputtering a layer of gold film on the silk fibroin film with a mask to obtain the silk fibroin-based optical anti-counterfeiting mark. In the invention a thin layer of Au film is sputtered on a silk fibroin film such that an anti-counterfeiting mark pattern appears at a high humidity, the method is simple and can significantly the production cost of optical anti-counterfeiting mark.
    Type: Grant
    Filed: July 25, 2016
    Date of Patent: December 8, 2020
    Assignee: NANTONG TEXTILE & SILK INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE
    Inventors: Keqin Zhang, Qingsong Li, Yu Peng, Ning Qi, Xiaohua Zhang
  • Patent number: 10859498
    Abstract: A method for visualization of conjunctival cells using fluoroquinolone antibiotics and a method for diagnosis of ocular lesions using the same. The method for visualization of conjunctival cells using fluoroquinolone antibiotics includes staining goblet cells of ocular conjunctiva with moxifloxacin, which is a fluoroquinolone antibiotic, and exciting the stained goblet cells with single photons in the near-UV region or in the visible region, followed by fluorescence photographing of the goblet cells, thereby enabling acquisition of morphological information on living tissue without damage to or destruction of the ocular conjunctiva.
    Type: Grant
    Filed: February 28, 2019
    Date of Patent: December 8, 2020
    Assignee: Postech Academy-Industry Foundation
    Inventors: Seonghan Kim, Ki Hean Kim, Myoung Joon Kim, Seunghun Lee, Hoon Cheol Jang, Viet Hoan Le, Soo Hyun Park
  • Patent number: 10858690
    Abstract: Provided herein are porous polymer monolith materials and processes that enable integration of blood fractionation, specific nucleic acid amplification and/or detection of nucleic acids from whole blood.
    Type: Grant
    Filed: May 25, 2017
    Date of Patent: December 8, 2020
    Assignee: Monolythix, Inc.
    Inventors: Ivan Rueda, Ian McFadden, Mark D. Dobbs, Victor Shum, Keith A. Oberg, Anthony Spence, Walt Caldwell
  • Patent number: 10837957
    Abstract: The present invention generally relates to in vitro methods for mimicking in vivo pathological or physiologic conditions. The methods comprise applying shear forces to a cell type or cell type plated on a surface within a cell culture container. Methods for testing drugs or compounds in such systems are also described.
    Type: Grant
    Filed: May 19, 2017
    Date of Patent: November 17, 2020
    Assignee: HemoShear, LLC
    Inventors: Brett R. Blackman, Brian R. Wamhoff, Ajit Dash, Michael B. Simmers, Ryan E. Feaver
  • Patent number: 10841503
    Abstract: An image capture system and method for imaging biological samples disposed in culture media supported by a plate. The system has a calibration module, an image acquisition module and an image presentation module. When the system receives a culture plate for imaging, default values for the culture plate and media are used to begin image acquisition at a given time. The captured image is then used to create a pixel by pixel map of the image. The system inspects the pixel-by-pixel map for saturated pixels and for signal to noise ratio and acquires a new image if the number of saturated pixels is at or above a predetermined threshold or the signal to noise ratio for the pixel is below a predetermined threshold. From this inspection a new value of photon flux and/or exposure time is determined and a new image is captured using the new value and the steps are repeated.
    Type: Grant
    Filed: December 13, 2018
    Date of Patent: November 17, 2020
    Assignee: BD KIESTRA B.V.
    Inventors: Raphael R. Marcelpoil, Cedrick Orny, Didier Morel
  • Patent number: 10815125
    Abstract: Polymers having pendant polycyclic aromatic hydrocarbon (PAH) groups covalently bound to the polymer backbone via thioester bonds are provided. The PAH groups are covalently bound to the backbone of the polymer by a molecular linker that includes a thioester bond. Also provided are dispersions of polymer-coated carbon nanotubes and carbon nanotube films formed from the dispersions.
    Type: Grant
    Filed: January 5, 2018
    Date of Patent: October 27, 2020
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Padma Gopalan, Michael Scott Arnold, Catherine Kanimozhi Kansiusarulsamy, Matthew John Shea
  • Patent number: 10808384
    Abstract: A well management system provides Internet-enabled monitoring, configuration, and control of a well system that may include a pump and a pressure tank. A controller is operatively coupled to the pump. The controller controls the pump based on pressure readings from a pressure sensor to maintain a configured pressure range within the system. The controller can communicate with a management system via a communications network. The management system is further in communication with one or more client devices, which may be associated with a homeowner, a service provider, a manufacturer, or the like. Via the management system, the client devices can receive information from the controller and send commands to the controller.
    Type: Grant
    Filed: July 26, 2018
    Date of Patent: October 20, 2020
    Assignee: AMTROL LICENSING INC.
    Inventors: Christopher A. Van Haaren, Michael Cogliati
  • Patent number: 10791960
    Abstract: Described are methods and uses employing metabolomic data to diagnose an asthma disease state or a Chronic Obstructive Pulmonary Disease (COPD) state. Further described are methods of uses of employing metabolomic data to distinguish between asthma and COPD. In particular, urinary metabolomic profiles are employed to enable differential diagnosis of asthma and COPD.
    Type: Grant
    Filed: June 3, 2016
    Date of Patent: October 6, 2020
    Assignee: University of Saskatchewan
    Inventors: Darryl Adamko, Anas El-Aneed
  • Patent number: 10788308
    Abstract: Aspects of the subject technology relate to particulate matter sensors for electronic devices. A particulate matter sensor may include three lasers, three total-internal-reflection lenses, and three detectors for detecting changes in the operation of the three lasers due to the principles of self-mixing interferometry. The three total-internal-reflection lenses may use internally reflective surfaces to tilt the three beams into three corresponding directions that form an orthogonal basis in the three dimensional space, so that a gas flow speed can be determined while maintaining a small, modular form factor for implementation of the sensor in portable electronic devices.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: September 29, 2020
    Assignee: Apple Inc.
    Inventors: Mehmet Mutlu, James J. Dudley, Mark T. Winkler, Wenrui Cai
  • Patent number: 10780436
    Abstract: A multi-channel microfluidic device for multi-parallel analyte detection includes a substrate and a multi-channel microfluidic assembly formed in the substrate. The multi-channel microfluidic assembly comprises a synchronized port; a plurality of separate ports; a plurality of channels arranged in parallel, where each of the plurality of channels includes a first end and a second end opposite to the first end; a first branch channel assembly; and a plurality of second branch assemblies. The synchronized port is connected with all the first ends of the plurality of the channels via the first branch channel assembly. Each of the plurality of the separate ports is in connection with the second end of each of the plurality of the channels via each of the plurality of the second branch channel assemblies.
    Type: Grant
    Filed: September 26, 2018
    Date of Patent: September 22, 2020
    Assignee: Advanced NanoBio Technologies Limited
    Inventors: Yeaun Jau Liou, Nien-Tsu Huang
  • Patent number: 10780185
    Abstract: A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Photoacoustic emission from the construct correlates with cellular membrane potential.
    Type: Grant
    Filed: July 10, 2018
    Date of Patent: September 22, 2020
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: James B. Delehanty, Michael H. Stewart, Okhil Nag, Jeffrey R. Deschamps, Kimihiro Susumu, Eunkeu Oh, Lauren D. Field, Alexander L. Efros, Alan L. Huston, Igor L. Medintz, Philip E. Dawson, Nashaat Rasheed, Parag V. Chitnis, John R. Cressman
  • Patent number: 10768113
    Abstract: Embodiments generally relate to a system for reading fluorescent-labelled diagnostic assays for in-vitro diagnostic applications. The system comprises a receiving member adapted to receive a fluorescent-labelled diagnostic assay cartridge carrying a fluorescent-labelled diagnostic assay; at least one excitation module configured to illuminate the diagnostic assay, when the diagnostic assay cartridge is placed in the receiving member; a camera module for capturing an image of the illuminated diagnostic assay placed in the receiving member; a processor for receiving the captured image from the camera module and determining whether or not a target analyte was present in the diagnostic assay captured by the camera module; and memory storing firmware, the firmware including a brightness compensation module configured to adjust the intensity of an image of a diagnostic cartridge captured by the camera module, in order to emulate a uniform field of illumination over the diagnostic cartridge.
    Type: Grant
    Filed: July 10, 2019
    Date of Patent: September 8, 2020
    Assignee: Lumos Diagnostics IP PTY LTD
    Inventor: William Samuel Hunter
  • Patent number: 10768087
    Abstract: The invention relates to a counting chamber for a microscope, adjustable in height. The method measures a cell count at two different chamber heights and deduces the concentration of particles without an exact calibration of the absolute height, as the height difference gives a measurement of the “missing” volume and therefore an estimate of the particle concentration.
    Type: Grant
    Filed: January 30, 2017
    Date of Patent: September 8, 2020
    Assignee: MINITUB GMBH
    Inventors: Georg Wippenbeck, Christian Simmet
  • Patent number: 10761086
    Abstract: The present invention relates provides methods of predicting cell sensitivity to a test agent. In some embodiments, the cells are cultured in a culture medium having serum.
    Type: Grant
    Filed: April 27, 2016
    Date of Patent: September 1, 2020
    Assignee: Dana-Farber Cancer Institute, Inc.
    Inventors: Anthony Letai, Patrick Bhola, Jeremy Ryan
  • Patent number: 10760072
    Abstract: The present invention relates to a method to detect the interaction between a target ligand and a moiety of interest using an adenylate cyclase enzyme (AC) and calmodulin (CaM) as interacting partners, said method comprising: i) expressing in a suitable host cell: (a) a low number of molecules of a first chimeric polypeptide containing AC, and (b) a low number of molecules of a second chimeric polypeptide containing CaM, wherein said AC in said first chimeric polypeptide and/or said CaM in said second chimeric polypeptide has decreased affinity for its interacting partner, wherein said AC in said first chimeric polypeptide is fused to a moiety of interest and said CaM in said second chimeric polypeptide is fused to a target ligand, or conversely, and wherein, when said moiety of interest and said target ligand interact, said AC is activated, and ii) detecting the activation of said AC.
    Type: Grant
    Filed: July 15, 2015
    Date of Patent: September 1, 2020
    Inventor: Daniel Ladant
  • Patent number: 10752879
    Abstract: The present invention provides a culture method for culturing, in recesses (10), a population including two or more cells including a cell derived from a stem cell and a mesenchymal cell. The cell derived from a stem cell is a cell obtained by differentiating a stem cell in vitro. The cell is a cell of one or more types selected from the group consisting of an endodermal cell, an ectodermal cell, and a mesodermal cell. The population is cultured in the recesses (10) together with a vascular cell or a secretor factor. Each recess (10) includes a space in which cells are movable. When a volume of the space is represented by V mm3 and the number of mesenchymal cells seeded in the space is represented by N, V is 400 or less and N/V is in a range from 35 to 3000.
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: August 25, 2020
    Assignees: Corning Incorporated, Public University Corporation Yokohama City University
    Inventors: Yoko Ejiri, Hideki Taniguchi, Takanori Takebe
  • Patent number: 10748288
    Abstract: Methods and systems for determining quality of an oocyte to reach various reproductive milestones, including fertilizing, developing into a viable embryo (blastocyst), implanting into the uterus, and reaching a clinical pregnancy, through visual assessment (non-invasive) from a single image using artificial intelligence software.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: August 18, 2020
    Inventors: Dan Nayot, Rene Michael Bharti, Jim Meriano, Alexandr Krivoi
  • Patent number: 10744505
    Abstract: A microfluidic device for in vitro 3D cell culture experimentation comprises a body in which is provided a cell culture chamber that is at least partly filled with a scaffolding substance for maintaining a cell culture. In the body a fluid path is provided that communicates with the cell culture chamber for directing a fluid stream along the scaffolding substance. The culture chamber above the scaffolding substance opens into an access port which is provided at an outer top surface of the body to provide direct access to the scaffolding substance. The scaffolding substance forms a fluid flow barrier which separates the fluid path from the access port.
    Type: Grant
    Filed: May 20, 2016
    Date of Patent: August 18, 2020
    Assignee: INTECRYPT B.V.
    Inventor: Mikhail Alexandrovich Ponomarenko
  • Patent number: 10745425
    Abstract: Disclosed a chemiluminescence enhancer and a chemiluminescence immunodetection kit. The chemiluminescence enhancer is specifically a betaine and can be used for enhancing the luminance of a luminescent acridine substance. The chemiluminescence immunodetection kit comprising the chemiluminescence enhancer and a luminescent acridine substance features greatly enhanced luminescence signals and high detection sensibility, and can be widely applied to fields such as clinical diagnoses, scientific research, and sanitation analysis.
    Type: Grant
    Filed: July 1, 2016
    Date of Patent: August 18, 2020
    Assignee: SHENZHEN YHLO BIOTECH CO., LTD.
    Inventors: Kunhui Hu, Gang Wang, Fuzhen Xia, Chungen Qian, Liang Zhu, Dingbiao Zou
  • Patent number: 10730049
    Abstract: In accordance with an embodiment, a microfluidic chip including a first cells enrichment system and a second cells enrichment system is provided. Channel layouts of the first and the second cells enrichment systems are symmetric with respect to a reflection plane vertical to the microfluidic chip. Each of the first the second cells enrichment systems includes a first fluid channel, a second fluid channel, a sample channel, an inlet channel, and a filtration chamber. The sample channel of the first cells enrichment system has a first inner wall and a first outer wall. The sample channel of the second cells enrichment system has a second inner wall and a second outer wall. The first outer wall and the second outer wall are both far from the reflection plane. A distance between the first outer wall and the second outer wall is in the range from about 10 ?m to about 1 cm.
    Type: Grant
    Filed: May 10, 2018
    Date of Patent: August 4, 2020
    Assignee: MiCareo Taiwan Co., Ltd.
    Inventors: Hui-Min Yu, Wen-Feng Chung, Jui-Lin Chen
  • Patent number: 10724043
    Abstract: Systems and methods are provided for transfecting immune competent cells with RNA at high efficiency and viability.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: July 28, 2020
    Assignee: NantBio, Inc.
    Inventors: Marcos Sixto, Kayvan Niazi, Ting Wu, Chihwei Chang
  • Patent number: 10718756
    Abstract: Mitochondria are central to the intrinsic apoptotic pathway of programmed cell death. Commitment to cell death occurs upon mitochondrial outer membrane permeabilization, and dysregulation of this vital apoptotic control point is implicated in various pathologies. Provided herein are novel sensors which can detect, in real time and with great sensitivity, the release of protons from intact mitochondria caused by mitochondrial outer membrane permeabilization. Embodiments include graphene based ion-sensitive field effect transistors.
    Type: Grant
    Filed: October 27, 2017
    Date of Patent: July 21, 2020
    Assignee: The Regents of the University of California
    Inventors: Peter Burke, Ted Pham, Phi Pham
  • Patent number: 10712268
    Abstract: This invention is in the field of fluorescence imaging and relates to a new near infrared (NIR) reactive oxygen species (ROS) sensor designed with controlled fluorescence on-off switching mechanism.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: July 14, 2020
    Assignee: University of Massachusetts
    Inventor: Maolin Guo
  • Patent number: 10709323
    Abstract: Disclosed is a method of identifying if a subject is sufficiently prepared to permit successful colonoscopic examination, the method comprising the steps of: (a) obtaining a stool sample from a subject having undergone preparation for a colonoscopic examination; (b) analysing the sample to detect the presence and/or amount of faecal matter, if any, in the stool sample; and (c) from the analysis in step (b), identifying whether the subject has been sufficiently prepared.
    Type: Grant
    Filed: February 5, 2018
    Date of Patent: July 14, 2020
    Assignee: Owlstone Medical Limited
    Inventors: Aditya Malkar, Lauren Brown, Marc Van Der Schee
  • Patent number: 10705092
    Abstract: A construct for detecting cellular membrane potential includes a nanoparticle operable as an electron donor; a modular peptide attached to the nanoparticle, the peptide comprising a nanoparticle association domain, a motif configured to mediate peptide insertion into the plasma membrane, and at least one attachment point for an electron acceptor positioned at a controlled distance from the nanoparticle; and an electron acceptor. The nanoparticle can be a quantum dot and the electron acceptor can be C60 fullerene. Emission correlates with cellular membrane potential.
    Type: Grant
    Filed: January 29, 2018
    Date of Patent: July 7, 2020
    Assignee: The Government of the United States of Americam as Represented by the Secretary of the Navy
    Inventors: James B. Delehanty, Michael H. Stewart, Okhil Nag, Jeffrey R. Deschamps, Kimihiro Susumu, Eunkeu Oh, Lauren D. Field, Alexander L. Efros, Alan L. Huston, Igor L. Medintz, Philip E. Dawson