Involving Viable Micro-organism Patents (Class 435/29)
  • Patent number: 11881311
    Abstract: In various embodiments, the present description relates to the use of factors related to survival. The methods, compositions and systems described herein may be used to determine factors affecting survival, assess survival risk based on factors related to survival and/or make suggestions to increase the likelihood of survival longer than otherwise predicted.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: January 23, 2024
    Assignee: BioAge Labs, Inc.
    Inventors: Kristen Patricia Fortney, Yonatan Nissan Donner, Eric Kim Morgen, Jonah Daniel Sinick, Andrew Jarai Ho
  • Patent number: 11867688
    Abstract: The present invention relates provides methods of predicting cell sensitivity to a test agent. In some embodiments, the cells are cultured in a culture medium having serum.
    Type: Grant
    Filed: July 27, 2020
    Date of Patent: January 9, 2024
    Assignee: DANA-FARBER CANCER INSTITUTE, INC.
    Inventors: Anthony Letai, Patrick Bhola, Jeremy Ryan
  • Patent number: 11858008
    Abstract: Systems and methods for particle sorting are presented including a monitoring system downstream of a particle separator or sorter. The system can utilize the monitoring system to adjust or calibrate operational parameters of the system in real time. When a particle of interest is mis-sorted, the probability is high that the particle of interest has been sorted into a non-targeted sortable unit that was adjacent in sequence to the sortable unit that was expected to include the particle of interest. The monitoring system monitors non-targeted sortable units in the system that were adjacent in sequence to targeted sortable units that are predicted to contain particles of interest. Signals from the monitoring system enable automated adjustment or calibration of operational parameters of the system such as sort delay or purity mask parameters.
    Type: Grant
    Filed: March 28, 2022
    Date of Patent: January 2, 2024
    Assignee: CYTONOME/ST, LLC
    Inventors: Gary Durack, William J. Williams, V
  • Patent number: 11850324
    Abstract: The present invention relates to bioinks based on undenatured collagen, kits containing the same, and methods of printing three-dimensional structures using the bioinks.
    Type: Grant
    Filed: October 12, 2017
    Date of Patent: December 26, 2023
    Assignee: Advanced Biomatrix, Inc.
    Inventors: David Bagley, Bowman Bagley, Dale Peterson
  • Patent number: 11841362
    Abstract: The present invention is related to a method for diagnosing Niemann-Pick disease in a subject comprising a step a), wherein the step a) comprises detecting a biomarker in a sample from the subject.
    Type: Grant
    Filed: December 22, 2021
    Date of Patent: December 12, 2023
    Assignee: Centogene GmbH
    Inventors: Arndt Rolfs, Hermann Mascher
  • Patent number: 11796449
    Abstract: A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
    Type: Grant
    Filed: February 19, 2019
    Date of Patent: October 24, 2023
    Assignee: ABS Global, Inc.
    Inventors: David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
  • Patent number: 11782050
    Abstract: The present disclosure relates to, inter alia, cell-based assays, recombinant cell lines, and methods for quantifying the potency and efficacy of cannabinoids and/or terpenoids.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: October 10, 2023
    Assignee: CannaMetrix, LLC
    Inventor: Harold C. Smith
  • Patent number: 11773375
    Abstract: The present invention is in the field of pluripotent stem cells, more particularly cardiomyocytes derived from pluripotent stem cells. The present invention provides a novel method for differentiating human pluripotent stem cells into a population of cardiomyocytes having an atrial phenotype, and use of said atrial cardiomyocytes for screening of drugs, AF disease model, and others. The method of the invention is particularly useful to generate cardiomyocytes having a more developed or mature atrialphenotype and/or to generate higher yield of cardiomyocytes having an atrialphenotype.
    Type: Grant
    Filed: March 27, 2017
    Date of Patent: October 3, 2023
    Assignee: Ncardia B.V.
    Inventors: Stefan Robbert Braam, Ana Catarina Martins Grandela, Karin Langenberg
  • Patent number: 11726081
    Abstract: The present disclosure provides methods for identifying compounds that cause structural changes in tau protein monomer and oligomer conformation. The methods include the use of cells that include tau proteins labeled with one or more chromophores, and exposing the cells to a test compound. The method further includes detecting a change in fluorescence resonance energy transfer (FRET) between the chromophores.
    Type: Grant
    Filed: February 13, 2020
    Date of Patent: August 15, 2023
    Assignee: REGENTS OF THE UNIVERSITY OF MINNESOTA
    Inventors: Jonathan N. Sachs, Chih Hung Lo
  • Patent number: 11714076
    Abstract: Methods for determining the toxicity of fresh-water and marine sediments and sediment pore water containing indigenous or introduced toxicants from each as a one-time analysis and/or for analysis over a period of time are provided. The present disclosure further provides kits assembled for the afore-mentioned determination. The methods and kits can be used for analyzing sediment and pore water samples from, among other locations, all environments where species having a dormant life stage may exist including, for example, natural zooplankton.
    Type: Grant
    Filed: April 22, 2019
    Date of Patent: August 1, 2023
    Assignee: University of North Carolina Wilmington
    Inventor: Joseph A. Covi
  • Patent number: 11708397
    Abstract: A chemigenetic calcium indicator and a method of measuring calcium are provided. The chemigenetic calcium indicator includes a calcium-binding protein domain attached to a ligand binding protein domain. The method of measuring calcium includes administering a chemigenetic calcium indicator to a subject and determining changes in fluorescence, the chemigenetic calcium indicator including a ligand binding protein domain having a calcium-binding protein domain and a dye-ligand conjugate attached thereto.
    Type: Grant
    Filed: December 31, 2018
    Date of Patent: July 25, 2023
    Assignee: HOWARD HUGHES MEDICAL INSTITUTE
    Inventors: Eric R Schreiter, Luke D. Lavis, Claire Deo, Hersh Bhargava, Ahmed Abdelfattah
  • Patent number: 11703427
    Abstract: The present disclosure provides methods and systems for cell and bead processing or analysis. A method for processing a cell or bead may include subjecting a bead to conditions sufficient to change a first characteristic or set of characteristics (e.g., cell or bead size). Such a method may further include subjecting the cell or bead to conditions sufficient to change a second characteristic or set of characteristics. In some cases, crosslinks may be formed within the cell or bead.
    Type: Grant
    Filed: June 7, 2019
    Date of Patent: July 18, 2023
    Assignee: 10X GENOMICS, INC.
    Inventors: Christopher Hindson, Andrew D. Price, Michael Schnall-Levin
  • Patent number: 11666914
    Abstract: A cartridge, in particular a disposable cartridge, for use in an electrowetting sample processing system. The cartridge contains an internal gap with at least one hydrophobic surface for enabling an electrowetting induced movement of a microfluidic droplet that has magnetic beads and further has a bead accumulation zone, into which the microfluidic droplet is transferable by electrowetting force and the magnetic beads are exposable to a magnetic force of a bead manipulation magnet. The internal gap has a bead extraction opening adjacent to the bead accumulation zone. The bead extraction opening provides a passage from the gap to an exterior space of the cartridge and is configured to removably receive the bead manipulation magnet for enabling an extraction of the magnetic beads from the microfluidic droplet by a removal of the bead manipulation magnet.
    Type: Grant
    Filed: May 9, 2018
    Date of Patent: June 6, 2023
    Assignee: TECAN TRADING AG
    Inventors: Patrick Kinney, Sujata Iyer, Tin Ngo, Jennifer Ji, Marta Matvienko, Tiffany Ding (Lay)
  • Patent number: 11656235
    Abstract: The subject invention provides materials and methods for single-step detection of target molecules in a sample. The methods and assays of the subject invention employ a dye-displacement strategy, in which aptamers complexed with a cyanine dye for sensitive and rapid detection of targets of interest. In the presence of a target, aptamer-target binding liberates the non-covalently bound aptamer-binding dye, resulting in optical changes that can be observed spectrophotometrically or with the naked eye. The methods and assays of the subject invention enable the colorimetric detection of targets of interest regardless of their structure, sequence, target-binding affinity, and physicochemical properties of their targets.
    Type: Grant
    Filed: August 7, 2021
    Date of Patent: May 23, 2023
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Yi Xiao, Obtin Alkhamis, Haixiang Yu
  • Patent number: 11629330
    Abstract: The invention encompasses methods for reducing the proportion of sperm cells with abnormal morphology, and unviable sperm cells, in a sperm cell population.
    Type: Grant
    Filed: March 18, 2020
    Date of Patent: April 18, 2023
    Assignee: Inguran, LLC
    Inventors: Juan Moreno, Kenneth Michael Evans, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
  • Patent number: 11629320
    Abstract: Zebrafish are a powerful model for investigating cardiac repair due to their unique regenerative abilities, scalability, and compatibility with many genetic tools. However, characterizing the regeneration process in live adult zebrafish hearts has proved challenging because adult fish are opaque and explanted hearts in conventional culture conditions experience rapid declines in morphology and physiology. To overcome these limitations, we fabricated a fluidic device for culturing explanted adult zebrafish hearts with constant media perfusion that is also compatible with live imaging. Unlike hearts cultured in dishes for one week, the morphology and calcium activity of hearts cultured in the device for one week were largely similar to freshly explanted hearts.
    Type: Grant
    Filed: October 16, 2019
    Date of Patent: April 18, 2023
    Assignees: University of Southern California, Children's Hospital Los Angeles
    Inventors: Megan McCain, Joycelyn Yip, Ching-Ling Lien, Michael Harrison
  • Patent number: 11613742
    Abstract: Provided herein are CasX:gNA systems comprising CasX polypeptides, guide nucleic acids (gNA), and optionally donor template nucleic acids useful in the modification of a SOD1 gene. The systems are also useful for introduction into cells, for example eukaryotic cells having mutations in the SOD1 protein or the SOD1 regulatory element. Also provided are methods of using such CasX:gNA systems to modify cells having such mutations and utility in methods of treatment of a subject with a SOD1-related disease.
    Type: Grant
    Filed: September 23, 2021
    Date of Patent: March 28, 2023
    Assignee: SCRIBE THERAPEUTICS INC.
    Inventors: Benjamin Oakes, Sean Higgins, Hannah Spinner, Sarah Denny, Brett T. Staahl, Kian Taylor, Katherine Baney, Isabel Colin, Maroof Adil, Cole Urnes
  • Patent number: 11604141
    Abstract: The compounds relate to zinc-sensitive fluorescent probes, compositions and methods utilizing the same. Such compounds provide an emission-ratiometric fluorescence response upon binding of an analyte. In some embodiments, compounds can be used for two-photon excitation microscopy or conventional fluorescence microscopy. The compounds described herein can also contain one or more functional groups to improve the emission-ratiometric fluorescence response.
    Type: Grant
    Filed: June 12, 2018
    Date of Patent: March 14, 2023
    Assignee: Georgia Tech Research Corporation
    Inventors: Christoph J. Fahrni, Adam M. McCallum, Michael Thomas Morgan
  • Patent number: 11591561
    Abstract: Transfer of genetic and other materials to cells is conducted in a hands-free, automated and continuous process that includes flowing the cells between electroporation electrodes to facilitate delivery of a payload into the cells, while acoustophoretically focusing the cells. Also described is a control method for the acoustophoretic focusing of cells that includes detecting locations of cells flowing through a channel, such as with an image analytics system, and modulating a drive signal to an acoustic transducer to change the locations of the cells flowing in the channel. Finally, an electroporation driver module is described that uses a digital to analog converter for generating an electroporation waveform and an amplifier for amplifying the electroporation waveform for application to electroporation electrodes.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: February 28, 2023
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Vishal Tandon, Charles A. Lissandrello, Ryan A. Dubay, Rebecca Christianson, Jenna Leigh Balestrini, Peter Hsi, Jason Fiering
  • Patent number: 11585806
    Abstract: The present invention relates to detection molecules comprising at least one binding molecule, at least one linker and at least one label, and detection methods making use of same. The invention provides a high-throughput method for detection, isolation and/or identification of specific entities or cells.
    Type: Grant
    Filed: June 15, 2015
    Date of Patent: February 21, 2023
    Assignees: Immudex ApS, Herlev Hospital
    Inventors: Henrik Pedersen, Søren Jakobsen, Sine Reker Hadrup, Amalie Kai Bentzen, Kristoffer Haurum Johansen
  • Patent number: 11565094
    Abstract: Disclosed herein are devices for use in transplanting cells. The devices can include a housing defining a cavity; and a support structure separating the cavity into a cell chamber and a reservoir chamber, wherein the support structure comprises a membrane for fluid communication between the cell chamber and reservoir chamber. The cell chamber can define a first opening comprising a microstructure containing an array of micro-channels, each having a diameter to facilitate growth of vascular tissues; and an array of micro-reservoirs, each having a diameter to facilitate housing of cell aggregates individually. The membrane can define a surface area that is at least 50% of a total surface area of the support structure. Methods of treating a subject for a disease condition, such as diabetes, are also disclosed.
    Type: Grant
    Filed: October 17, 2018
    Date of Patent: January 31, 2023
    Assignee: THE METHODIST HOSPITAL SYSTEM
    Inventors: Marco Farina, Andrea Ballerini, Daniel W. Fraga, Usha Ramachandran Thekkedath, Omaima M. Sabek, Ahmed Osama Gaber, Alessandro Grattoni
  • Patent number: 11566216
    Abstract: A cell culture insert with a hollow cylindrical housing having an upper end-face opening delimited by an opening edge and a lower end-face base designed as a membrane, support feet being arranged on the base edge of the housing and/or on the base and at least one support arm. The legs protrude downward for supporting the housing on a support in a non-tipping manner with a small uniform spacing between the base and the support. The at least one support arm protrudes outwards at the opening edge and can be placed on an edge of a well plate in which a plurality of wells. The support arm is an edge hanging element, and for this purpose, has a support section that runs radially relative to the housing and a hanging section that runs from the support section downwards in the direction of the base of the housing.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: January 31, 2023
    Assignee: BRAND GMBH + CO KG
    Inventors: Frank Nicolas, Antonio Romaguera, Peter Prokopp
  • Patent number: 11555773
    Abstract: An inflammatory marker is calculated using a nonlinear function including, as variables, a parameter associated with an erythrocyte aggregation and another parameter associated with an erythrocyte density. The parameter associated with the erythrocyte aggregation is calculated based on a syllectogram measured from a blood specimen. The parameter associated with the erythrocyte density is measured from the blood specimen.
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: January 17, 2023
    Assignee: NIHON KOHDEN CORPORATION
    Inventor: Makoto Higuchi
  • Patent number: 11547995
    Abstract: The invention(s) cover systems and methods for target detection in a multiplexed and rapid manner. Embodiments of the system can include: a base substrate; and an array of sample processing regions defined at a broad surface of the base substrate, wherein each of the array of sample processing regions includes: a set of microwell subarrays arranged in a gradient by volumetric capacity between an upstream end and a downstream end of each respective sample processing region, and a boundary separating each respective sample processing region from adjacent sample processing regions. The system can support methods, with example implementation by an automated platform, for returning preliminary results from a subset of microwells of the samples processing regions, as well as results pertaining to specific and non-specific amplification, for multiple targets of a sample.
    Type: Grant
    Filed: October 12, 2021
    Date of Patent: January 10, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Kalyan Handique, Ronald Lebofsky
  • Patent number: 11543349
    Abstract: Method for calibrating or monitoring performance of an optical measurement device that includes using a robotic arm to move a reference device having an optical reference material into a signal-detecting position of the optical measurement device. With the optical measurement device, detecting an emission emitted by the optical reference material of the reference device in the signal-detecting position. Then generating a reference signal representing a characteristic of the emission detected by the optical measurement device and comparing the reference signal to an expected reference signal for the emission to calibrate or monitor the performance of the optical measurement device.
    Type: Grant
    Filed: May 17, 2021
    Date of Patent: January 3, 2023
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Norbert D. Hagen, David Opalsky, George T. Walker, Byron J. Knight
  • Patent number: 11542471
    Abstract: The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on.
    Type: Grant
    Filed: August 14, 2018
    Date of Patent: January 3, 2023
    Assignees: SUMITOMO CHEMICAL COMPANY, LIMITED, RIKEN
    Inventors: Tokushige Nakano, Satoshi Ando, Yoshiki Sasai, Mototsugu Eiraku
  • Patent number: 11504810
    Abstract: In order to cut a plurality of clumps having an approximately uniform shapes and approximately uniform dimensions out of a cell aggregate which has proliferated and appropriately eliminate contamination with fragments of different shapes or dimensions, when cutting the clumps of approximately uniform shape out of the cell aggregate which has proliferated, cutting lines along which the clumps of a specific shape are cut out are set such that the area of a peripheral part of the cell aggregate which is not cut by the cutting line exceeds the surface area of one of the clumps, and the cell aggregate is cut by irradiating with laser light in such a way as to trace the cutting lines.
    Type: Grant
    Filed: April 5, 2016
    Date of Patent: November 22, 2022
    Assignee: KATAOKA CORPORATION
    Inventors: Masami Suzuki, Norio Nishi, Junichi Matsumoto
  • Patent number: 11484877
    Abstract: A blood sample collection and/or storage device includes a two-piece housing that encompasses a port at which a fingertip blood sample is collected at a sample port. After the sample is taken, the two-piece housing is moved to a closed position deposit the sample onto a storage membrane Embodiments of the device include a one or more capillaries with movable plungers that dispense fluid from the sample port onto the membrane as the housing is closed. A desiccant, which may be held in place by a backbone structure within the housing, is positioned adjacent the membrane. The housing may also be opened to access the stored sample for further processing.
    Type: Grant
    Filed: May 24, 2019
    Date of Patent: November 1, 2022
    Assignee: WEAVR HEALTH CORP.
    Inventor: Brandon T. Johnson
  • Patent number: 11473061
    Abstract: Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.
    Type: Grant
    Filed: February 1, 2017
    Date of Patent: October 18, 2022
    Assignee: Cedars-Sinai Medical Center
    Inventors: Robert Barrett, Clive Svendsen, Stephan R. Targan, Michael Workman, Dhruv Sareen, Uthra Rajamani
  • Patent number: 11462785
    Abstract: A battery device (100) is disclosed, comprising a plurality of cells (110), an enclosure (120) configured to accommodate cells when they are at least partly immersed in a thermal management liquid, and at least one flow unit (130) arranged within the enclosure to control a flow of the thermal management liquid through the enclosure. The at least one flow unit comprises a first electron (131) and a second electrode (132) that are arranged offset from each other and being connectable to a voltage source so as to affect the flow between the electrodes.
    Type: Grant
    Filed: June 24, 2019
    Date of Patent: October 4, 2022
    Inventors: Are Björneklett, Peter Nilsson, Robert Thorslund
  • Patent number: 11422091
    Abstract: This invention is in the field of fluorescence imaging and relates to a new near infrared (NIR) reactive oxygen species (ROS) sensor designed with controlled fluorescence on-off switching mechanism.
    Type: Grant
    Filed: June 3, 2020
    Date of Patent: August 23, 2022
    Assignee: UNIVERSITY OF MASSACHUSETTS
    Inventor: Maolin Guo
  • Patent number: 11419499
    Abstract: A device for cancer screening and triage using an optical coherence tomography (OCT) imaging system integrated with optical imaging probe is provided. Endoscopic OCT images are generated using the OCT system having a helical probe. The images are further analyzed to generate a depth resolved intensity OCT signal to classify the region of tissue into variable grades of dysplasia to guide the physician's biopsy or resection.
    Type: Grant
    Filed: January 22, 2019
    Date of Patent: August 23, 2022
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Andrew Emon Heidari, Zhongping Chen, Bryan S. Benn
  • Patent number: 11415503
    Abstract: A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.
    Type: Grant
    Filed: October 7, 2021
    Date of Patent: August 16, 2022
    Assignee: ABS GLOBAL, INC.
    Inventors: David Appleyard, Jeff Betthauser, Marjorie Faust, John Larsen, Guocheng Shao, Zheng Xia, Yu Zhou
  • Patent number: 11416991
    Abstract: A system for identification of bacterial cells in free solution in a sample. A sample handler is adapted to position the sample. A light source illuminates a large volume of the sample. An imager is located to receive light scattered from the sample. A computer it is coupled to receive data transmitted from the imager. A controller is coupled to send control signals to the sample handler and the computer. The imager processes the scattered light to form images of the bacteria and transmits bacteria image information to the computer, wherein the bacteria image information includes intensity values and position data for the bacteria images from which the computer determines the presence of bacteria.
    Type: Grant
    Filed: September 4, 2018
    Date of Patent: August 16, 2022
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    Inventors: Nongjian Tao, Yunze Yang
  • Patent number: 11408825
    Abstract: A forensic detector for remotely sensing a porphyrin-containing substance on a surface of an object is provided. The forensic detector includes an excitation light source for generating an excitation light beam, plural optical elements, one or more sensors for measuring a first and second radiation powers, and a computer processor. The excitation light beam has an excitation wavelength capable of exciting porphyrin to luminesce at a target wavelength centered between 665-675 nm. The first and second radiation powers are in non-overlapping wavelength regions of the object-originated light beam. The computer processor is configured to compute a SNR according to the first radiation power and a background noise power level, and to determine if the porphyrin-containing substance is present on the surface according to the SNR. The background noise power level is obtained from the one or more second radiation powers.
    Type: Grant
    Filed: August 28, 2020
    Date of Patent: August 9, 2022
    Assignee: Hong Kong Applied Science and Technology Research Institute Company Limited
    Inventor: Hoi Ching Cheung
  • Patent number: 11401463
    Abstract: The present invention concerns a luminescent particle comprising a nanoparticle of formula A1-xLnxVO4(1-y)(PO4)y??(I) in which A is selected from yttrium (Y), gadolinium (Gd), lanthanum (La), and mixtures thereof; Ln is selected from europium (Eu), dysprosium (Dy), samarium (Sm), neodymium (Nd), erbium (Er), ytterbium (Yb), and mixtures thereof; 0<x<1; and 0?y<1; characterized in that the nanoparticle on its surface has tetraalkylammonium cations in an amount such that said nanoparticle has a zeta potential, ?, of less than or equal to ?28 mV in an aqueous medium with a pH?5, more particularly with a pH?5.5, and with an ionic conductivity >100 ?S·cm?1. It also concerns a method for preparing such luminescent particles, a colloidal suspension of these particles, and the use thereof as a diagnostic agent, and also a diagnostic kit comprising such luminescent particles.
    Type: Grant
    Filed: May 18, 2018
    Date of Patent: August 2, 2022
    Assignees: ECOLE POLYTECHNIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
    Inventors: Thierry Gacoin, Maximilian Richly, Pascal Preira, Antigoni Alexandrou, Cédric Bouzigues, Rabei Mohammedi
  • Patent number: 11396644
    Abstract: In related-art methods of differentiating pluripotent stem cells into a desired cell type, there has not been established a differentiation induction method using human ES/iPS cells and being highly efficient. Many attempts have been made, including a stepwise differentiation induction method based on the control of culture conditions or the addition of, for example, various cell growth factors/differentiation factors to a culture solution, but the use of complicated culture steps is a big problem. A method of inducing differentiation into a desired cell type within a short period of time and with high efficiency by use of a pluripotent stem cell that actively undergoes cell differentiation, which is obtained by reducing an undifferentiated state of the pluripotent stem cell, has been developed, and thus the present invention has been completed.
    Type: Grant
    Filed: January 31, 2017
    Date of Patent: July 26, 2022
    Assignee: KEIO UNIVERSITY
    Inventor: Minoru Ko
  • Patent number: 11370823
    Abstract: Compositions and provided to induce cells of the inner ear to renter the cell cycle and to proliferate. In particular, hair cells are induced to proliferate by administration of a composition which activates the Myc and Notch. Supporting cells are induced to transdifferentiate to hair cells by inhibition of Myc and Notch activity or the activation of Atoh1. Methods of treatment include the intracellular delivery of these molecules to a specific therapeutic target.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: June 28, 2022
    Assignees: Massachusetts Eye and Ear Infirmary, President and Fellows of Harvard College
    Inventors: Zheng-Yi Chen, David R. Liu, Margie Li, David B. Thompson, John Zuris
  • Patent number: 11371022
    Abstract: The invention relates to a method of creating a human blood-brain barrier (BBB) model from the differentiation of human pluripotent stem cells (hPSCs), wherein the BBB exhibits sustained transendothelial electrical resistances (TEER) over 2000 ?·cm2 for at least 3 days after seeding.
    Type: Grant
    Filed: February 16, 2017
    Date of Patent: June 28, 2022
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Tae-Eun Park, Anna Herland, Edward Anthony Fitzgerald, Donald Elliot Ingber
  • Patent number: 11366299
    Abstract: A method for illuminating a sample slice uses a light beam or a light sheet during single plane illumination microscopy (SPIM). The light beam or light sheet is deflected by an angle mirror having a first and second reflective surface reflecting a first and second portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the deflecting. Alternatively, the light beam or light sheet is refracted by a refractive optical component comprising a first and second refractor surface refracting a first portion of the light beam or light sheet, respectively, whereby the first and second portions of the light beam or light sheet spatially overlap one another after the refracting.
    Type: Grant
    Filed: October 10, 2016
    Date of Patent: June 21, 2022
    Assignee: LEICA MICROSYSTEMS CMS GMBH
    Inventor: Felix Neugart
  • Patent number: 11362236
    Abstract: The present disclosure provides a quantum dot and a manufacturing method for the same, and a luminescent material, a light-emitting element and a display device applying the quantum dot. The quantum dot includes a core and a shell layer. The core is at least one selected from the group consisting of a XII-XV group compound semiconductor nano-crystal, a XII-XVI group compound semiconductor nano-crystal, a XIII-XV group compound semiconductor nano-crystal and a XIII-XVI group compound semiconductor nano-crystal. The core contains a cadmium element and a selenium element. The shell layer contains a zinc element and a sulfur element. The shell layer encloses the core.
    Type: Grant
    Filed: November 12, 2019
    Date of Patent: June 14, 2022
    Assignee: CHIMEI CORPORATION
    Inventors: Wei-Ta Chen, Keng-Chu Lin
  • Patent number: 11352651
    Abstract: The invention relates to the isolation of polyunsaturated fatty acids (PUFAs) from a lipids containing biomass. The main characteristics of the method are that a suspension of cells containing PUFAs is obtained and the cells are optionally lysed. The suspension is concentrated and then incubated for at least an hour at a controlled temperature while adding a specific range of base equivalents to biomass and maintaining a moderate pH. The isolation of lipid may be performed in the absence of organic solvents and without the addition of salt.
    Type: Grant
    Filed: December 20, 2017
    Date of Patent: June 7, 2022
    Assignees: Evonik Operations GmbH, DSM IP Assets B.V.
    Inventors: Michael Diehl, Xiao Daniel Dong, Annika Hartmann, Martin Heining, Michael Benjamin Johnson, Jochen Lebert, Neil Francis Leininger, Kirt Lyvell Matthews, Sr., Mark Edward Nejako, II, Holger Pfeifer, Christian Rabe, Shannon Elizabeth Ethier Resop, Ginger Marie Shank, Vinod Tarwade, David Allen Tinsley
  • Patent number: 11352606
    Abstract: Low oxygen tension is a critical regulator of the developing or regenerating vasculature. The present invention is based on the determination that low oxygen tension during early stages of early vascular cell (EVC) derivation induces endothelial commitment and maturation of pluripotent stem cells. Inhibition of reactive oxygen species generation during the early stages of differentiation abrogates the endothelial inductive effects of the low oxygen environments. Methods of generating various types of cells from pluripotent stem cells (PSCs) are described, as well as compositions and methods of use thereof. In particular, generation of EVCs, bicellular vascular populations, early endothelial cells (ECs) and pericytes via culture in a low oxygen environment is described.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: June 7, 2022
    Assignee: The Johns Hopkins University
    Inventors: Sharon Gerecht, Sravanti Kusuma
  • Patent number: 11345890
    Abstract: The present invention features a neural organoid that recapitulates in vitro most characteristics of the brain (e.g., human), and methods of using this neural organoid to study disease and to identify therapeutic agents for the treatment of neurological diseases and disorders.
    Type: Grant
    Filed: January 12, 2017
    Date of Patent: May 31, 2022
    Inventor: Rene Anand
  • Patent number: 11345963
    Abstract: The present disclosure relates to compositions and methods for marking/tagging objects for identification. In particular, tagging objects with a nucleic acid taggant (genetic tag based merchandise authentication).
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: May 31, 2022
    Assignee: eBay Inc.
    Inventor: Sergio Pinzon Gonzales, Jr.
  • Patent number: 11340165
    Abstract: A sample observation device includes an imaging unit that images observation light generated due to irradiation with the planar light that is transmitted through a membrane filter and outputs fluorescent light image data, a partial image generation unit that specifies a first area corresponding to a first sample holding space and a second area corresponding to a second sample holding space in the fluorescent light image data, and generates first partial image data corresponding to the first area and second partial image data corresponding to the second area, an observation image generation unit that generates first observation image data and second observation image data on the basis of the partial image data, and an analysis unit that analyzes a sample on the basis of the first observation image data and the second observation image data.
    Type: Grant
    Filed: April 10, 2018
    Date of Patent: May 24, 2022
    Assignee: HAMAMATSU PHOTONICS K.K.
    Inventors: Norikazu Sugiyama, Masanori Matsubara, Satoshi Yamamoto
  • Patent number: 11340244
    Abstract: A method and apparatus for removing magnetic beads together with molecular targets of interest from a fluid is described. A consumable bead collection probe is placed in a vessel containing magnetic beads suspended in the fluid. A magnet outside the vessel concentrates magnetic beads on a surface of the collection probe so they may be manipulated in and out of vessels during washing or processing steps without transferring the fluid into or out of a vessel. In a further embodiment, a vibrational source is attached to the collection probe such that, in combination with the magnet in a position external to the vessel, beads may be washed without releasing them from the collection probe.
    Type: Grant
    Filed: January 28, 2020
    Date of Patent: May 24, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Hannah Pezzi
  • Patent number: 11326198
    Abstract: A method to classify and identify microorganisms using the FTIR ATR technique.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: May 10, 2022
    Assignee: ALIFAX S.R.L.
    Inventor: Paolo Galiano
  • Patent number: 11327002
    Abstract: A size reduction in an optical system that detects a fluorescent photon is achieved. A photon detection operation of a photon detection unit (21) is controlled on the basis of timing of an irradiation operation of an excitation light source (50), and after excitation light emitted to a microfluidic channel (10) is switched off, a fluorescent photon generated by a target flowing in the microfluidic channel (10) is detected.
    Type: Grant
    Filed: June 5, 2018
    Date of Patent: May 10, 2022
    Assignees: SHARP KABUSHIKI KAISHA, THE UNIVERSITY OF TOKYO
    Inventors: Kunihiko Iizuka, Yoshihisa Fujimoto, Takeshi Mitsunaka, Teruo Fujii, Soo-Hyeon Kim
  • Patent number: 11318459
    Abstract: A method for detecting the presence of a microorganism in a fluid sample is disclosed. The method includes providing a fluid specimen within a specimen collection container having a sidewall defining an interior therein. The interior includes a mechanical separator adapted for separating the fluid sample into first and second phases within the specimen collection container and a sensing element capable of detecting the presence of a microorganism disposed therein. The method includes subjecting the specimen collection container to applied rotational force to isolate a concentrated microorganism region. The method also includes detecting by a sensing element the presence or absence of a microorganism within the concentrated microorganism region.
    Type: Grant
    Filed: September 4, 2013
    Date of Patent: May 3, 2022
    Assignee: Becton, Dickinson and Company
    Inventors: Song Shi, Mei Yang, Klaus W. Berndt, Robert J. Losada, Jamieson W. Crawford, Ravi Attri, Christopher A. Battles, Benjamin R. Bartfeld, Gregory R. Hires