Involving Viable Micro-organism Patents (Class 435/29)
  • Patent number: 10663417
    Abstract: A magnetic resonance device for monitoring growth of tissue in one or more bioreactors. The device can include a first magnet and a second magnet that can form a uniform magnetic field of desired strength around at least one sample of effluent from at least one bioreactor. At the command of a controller, an RF signal can illuminate the at least one magnetized sample, and sensors can detect at least one echo signal from the at least one magnetized sample. The controller can characterize the at least one sample based on the at least one echo signal. A resonator can shape the at least one echo signal.
    Type: Grant
    Filed: November 30, 2018
    Date of Patent: May 26, 2020
    Assignee: DEKA Products Limited Partnership
    Inventors: David Blumberg, Jr., Michael C. Tilley, Derek G. Kane, David C. Nivens
  • Patent number: 10665445
    Abstract: A sample loading plate that includes at least one sample mounting spot that mount a sample thereon is provided with a substrate having a conductive surface and an insulating film that is laminated on the conductive surface of the substrate and that has at least an insulating surface, the insulating film being sparsely formed so that the conductive surface of the substrate is partially exposed at least in the sample mounting spot. Thus, a voltage applied to the sample loading plate can effectively place the sample in an electric field. As a result of which, in a mass spectrometric analysis of the sample, there is no charge up of the sample and appropriate ionization becomes possible.
    Type: Grant
    Filed: September 17, 2018
    Date of Patent: May 26, 2020
    Inventors: Kazuhiro Toriumi, Mitsunori Miyamoto, Satoru Fukumoto
  • Patent number: 10662405
    Abstract: A system for identifying Candida auris is disclosed. The system has two aspects. The first is a positive selection of C. auris based on C. auris's distinctive resistance to quaternary ammonium compounds (especially at elevated incubation temperatures). The second is a negative selection of C. auris based on C. auris's distinctive sensitive to tert-Butyl-hydroperoxide. C. auris can be identified in a sample through use of a positive-selection culture medium, which fosters C. auris colony growth while suppressing growth of other yeasts. The isolate can be confirmed as C. auris through use of a negative-selection culture medium, which suppresses C. auris colony growth while permitting growth of other yeasts.
    Type: Grant
    Filed: September 18, 2018
    Date of Patent: May 26, 2020
    Assignee: Santa Fe BioLabs LLC
    Inventor: Wolfram Siede
  • Patent number: 10655096
    Abstract: A method, apparatus, and system are provided for the printing and maturation of living tissue in an Earth-referenced reduced gravity environment such as that found on a spacecraft or on other celestial bodies. The printing may be three-dimensional structures. The printed structures may be manufactured from low viscosity biomaterials.
    Type: Grant
    Filed: August 1, 2016
    Date of Patent: May 19, 2020
    Assignee: Techshot, Inc.
    Inventors: John C. Vellinger, Eugene Boland, Michael A. Kurk, Krystal Milliner, Nester Samuel Logan
  • Patent number: 10648994
    Abstract: A device for processing and cataloging of a sample, in a container having a label with identifying data, has a frame; a plurality of cameras mounted to the frame; and a controller system coupled to the cameras, the controller system including a controller and a non-transitory memory storing instructions, which, when executed by the controller, cause carrying out of computer processes that produce a catalog record output characterizing the sample, such record also including the identifying data.
    Type: Grant
    Filed: June 25, 2019
    Date of Patent: May 12, 2020
    Assignee: VistaPath Biosystems Inc.
    Inventors: Timothy N. Spong, Benjamin T. Burley
  • Patent number: 10640751
    Abstract: A method of generating protein-induced pluripotent stem cells by delivering bacterially expressed reprogramming proteins into nuclei of starting somatic cells using the QQ-protein transduction technique, repeating several cell reprogramming cycles for creating reprogrammed protein-induced pluripotent stem cells, moving the reprogrammed cells into a feeder-free medium for expansion, and expanding and passaging the reprogrammed cells in a whole dish for generating homogeneous piPS cells. Also provided are the piPS cells formed using this method and uses thereof.
    Type: Grant
    Filed: February 8, 2019
    Date of Patent: May 5, 2020
    Assignee: Wayne State University
    Inventors: Jianjun Wang, Qianqian Li
  • Patent number: 10640807
    Abstract: Disclosed herein, inter alia, is method, kit and systems for detecting a pathogen infection in a bodily sample, the method comprising (i) staining said bodily sample with two or more dyes, comprising at least one dye predominantly staining DNA to thereby provide differential staining between DNA and at least one other cellular component being different from DNA; (ii) identifying at least a first stained area comprising the DNA, if exists in the sample, and at least one other stained area comprising the other cellular component; (iii) extracting structural features for the first stained area and the at least one other stained area, said structural features comprise at least one of (i) size of at least one of the first stained area and one other stained area and (ii) location of said first stained area and said at least one other stained area one with respect to the other; and (iv) determining the presence of a suspected pathogen in the bodily sample if a first stained area was identified and said structural fe
    Type: Grant
    Filed: December 27, 2012
    Date of Patent: May 5, 2020
    Assignee: S.D. Sight Diagnostics Ltd
    Inventors: Joseph Joel Pollak, Daniel Levner, Yonatan Bilu, Arnon Yafin, Noam Yorav-Raphael, Yuval Greenfield
  • Patent number: 10641761
    Abstract: A method of assaying metastasis can include: providing a device of one of the embodiments; introducing the at least one cancer cell into the at least one internal chamber or at least one fluid channel; and studying metastasis of the at least one cancer cell. Optionally: introducing cancer cells into a first internal chamber; detecting escape of the cancer cell from the first internal chamber into the fluid channel; detecting migration of the cancer cell through the fluid channel; detecting adhesion of the cancer cell to a coating on the fluid channel; detecting invasion of the cancer cell into a second internal chamber from the fluid channel; or visualizing metastasis of the cancer cell with a visualization device.
    Type: Grant
    Filed: February 15, 2018
    Date of Patent: May 5, 2020
    Assignee: CFD Research Corporation
    Inventors: Balabhaskar Prabhakarpandian, Kapil Pant
  • Patent number: 10634665
    Abstract: Disclosed herein are embodiments of a device that can be used to mimic the biochemical and physiological actions of a lung organ. Also disclosed herein are embodiments of components that are included in the device as well as methods of making and using the device. Further disclosed are platform device embodiments and various components used therein that can be used in combination with the lung organ devices disclosed herein. In some embodiments, the disclosed devices can be used to determine drug toxicity and also can be used with one or more disease models to determine methods of treating disease.
    Type: Grant
    Filed: September 24, 2015
    Date of Patent: April 28, 2020
    Assignees: Triad National Security, LLC, Vanderbilt University
    Inventors: Rashi Iyer, Jen-Huang Huang, Pulak Nath, Jennifer Harris, John P. Wikswo, Jr.
  • Patent number: 10625259
    Abstract: This disclosure describes single-use test cartridges, cell analyzer apparatus, and methods for automatically performing microscopic cell analysis tasks, such as counting and analyzing blood cells in biological samples. A small measured quantity of a biological sample, such as whole blood, is placed in a mixing bowl on the disposable test cartridge after being inserted into the cell analyzer. The analyzer also deposits a known amount of diluent/stain in the mixing bowl and mixes it with the blood. The analyzer takes a measured amount of the mixture and dispenses in a sample cup on the cartridge in fluid communication with an imaging chamber. The geometry of the imaging chamber is chosen to maintain the uniformity of the mixture, and to prevent cells from crowding or clumping as it is transferred into the imaging chamber by the analyzer. Images of all of the cellular components within the imaging chamber are counted and analyzed to obtain a complete blood count.
    Type: Grant
    Filed: June 7, 2017
    Date of Patent: April 21, 2020
    Assignee: Medica Corporation
    Inventors: Ronald Jones, Adrian Gropper, Robert Hagopian, Charles Rogers, Thomas Vitella, Tyler Cote, Donald Barry, Dirk Osterloh, Chen Yi
  • Patent number: 10626374
    Abstract: The present invention provides a new method for producing Engineered Heart Muscle (EHM) under chemically fully defined conditions and compounds all compatible with GMP regulations. The resulting human myocardium generates force and shows typical heart muscle properties.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: April 21, 2020
    Inventors: Wolfram-Hubertus Zimmermann, Malte Tiburcy, James Hudson
  • Patent number: 10628944
    Abstract: The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to methods and devices for determining the quality of an embryo. More specifically, the present invention relates to the use of three dimensional reconstructions for determining the quality of an embryo.
    Type: Grant
    Filed: July 1, 2015
    Date of Patent: April 21, 2020
    Inventors: Samir Hamamah, Elodie Scalici
  • Patent number: 10613078
    Abstract: The current disclosure relates to technology for predicting cardiotoxicity of compounds. In vitro methods using human stem-cell derived cardiomyocytes cultured in specific media are disclosed, as well as kits for use in the method. In the method, the cells are treated with a test compound in combination with a mitochondrial toxicant.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: April 7, 2020
    Assignee: NCARDIA B.V.
    Inventors: Stefan Robbert Braam, Fleur Stevenhagen
  • Patent number: 10590393
    Abstract: In one embodiment, the present invention is a method of creating a fully-human blood-brain barrier (BBB) model, comprising the steps of (a) obtaining a mixture of neural cells and brain microvascular endothelial cells (BMECs), wherein the neural cells and BMECs that comprise the mixture were produced from the differentiation of human pluripotent stem cells (hPSCs); (b) purifying BMECs from the mixture of neural cells and BMECs of step (a); and (c) co-culturing the purified BMECs with a cell type selected from the group consisting of pericytes, astrocytes and differentiated neural progenitor cells (NPCs), wherein a blood brain barrier model is created.
    Type: Grant
    Filed: March 11, 2013
    Date of Patent: March 17, 2020
    Inventors: Eric V. Shusta, Sean P. Palecek, Ethan S. Lippmann, Samira M. Azarin
  • Patent number: 10589275
    Abstract: A device for separating and concentrating target particles or molecules from a fibrinogen containing sample of liquid comprises a container (1) for collecting the sample and a closure (2). The container (1) comprises a first end and a second end and at least one interior wall defining a reservoir portion (5) for receiving the sample. The reservoir portion (5) comprises at least one anchor element (4) to locally catch a polymerized fibrin pellet formed upon the addition of the sample into the container. The separation and concentration process is operated by trapping the target particles or molecules into the so-formed polymerized fibrin pellet that are captured on the anchor element (4).
    Type: Grant
    Filed: June 1, 2015
    Date of Patent: March 17, 2020
    Inventors: Amar Rida, Sélène Ferrari
  • Patent number: 10585033
    Abstract: There are provided a microparticle measuring device capable of analyzing microparticles with increased accuracy and a microparticle analysis method. According to a microparticle measuring device 1, transmission images of microparticles in the liquid sample are captured by a plurality of image capturing units that are disposed in mutually different orientations with respect to a liquid feed pipe when viewed in a cross section orthogonal to the flowing direction of a liquid sample in the liquid feed pipe, and the microparticles are analyzed by an analyzing unit on the basis of the transmission images.
    Type: Grant
    Filed: December 7, 2018
    Date of Patent: March 10, 2020
    Assignee: Sumitomo Electric Industries, Ltd.
    Inventors: Takuya Okuno, Akinori Kimura, Ichiro Sogawa, Hiroshi Suganuma
  • Patent number: 10584366
    Abstract: Embodiments disclose apparatus, methods and software for performing biological screening and analysis implemented using an instrument platform capable of detecting a wide variety of cell-based secretions, expressed proteins, and other cellular components. The platform may be configured for simultaneous multiplexed detection of a plurality biological components such that a large number of discrete samples may be individually sequestered and evaluated to detect or identify constituents from the samples in a highly parallelized and scalable manner.
    Type: Grant
    Filed: December 3, 2015
    Date of Patent: March 10, 2020
    Assignee: IsoPlexis Corporation
    Inventors: Patrick Paczkowski, Sean MacKay, Sean McCusker
  • Patent number: 10584153
    Abstract: The present application discloses a method of growing or proliferating nerve cells by contacting the cells with phosphatase and tensin homolog (PTEN) lipid phosphatase inhibiting peptide.
    Type: Grant
    Filed: April 29, 2016
    Date of Patent: March 10, 2020
    Assignee: Kolon TissueGene, Inc.
    Inventors: Kwan Hee Lee, Moon Jong Noh, Kwangwook Ahn
  • Patent number: 10577583
    Abstract: Provided herein are methods of perfusion culturing an adherent mammalian cell using a shake flask and a plurality of microcarriers, and various methods that utilize these culturing methods.
    Type: Grant
    Filed: January 18, 2018
    Date of Patent: March 3, 2020
    Assignee: Genzyme Corporation
    Inventors: Jianguo Yang, Yang Yang, Jennifer Tengtrakool, Weichang Zhou
  • Patent number: 10577580
    Abstract: Described herein are new approaches for the selection of S. cerevisiae strains with increased ethanol production from hydrolyzed starch derived sugars. An industrial production strain of Saccharomyces cerevisiae AS400 was subjected to positive selection of mutants resistant to toxic concentrations of oxythiamine, trehalose, 3-bromopyruvate, glyoxylic acid, and glucosamine. The selected mutants are characterized by 5-8% increase in ethanol yield (g g?1 of consumed glucose) as compared to the parental industrial ethanol-producing strain. A multiple-step selection approach that consisted of the sequential selection using glyoxylic acid, glucosamine and bromopyruvate as selective agents resulted in a 12% increase in ethanol yield during fermentation on industrial media. These results indicate that the selection methods provided herein are useful for producing a variety of strains that are promising candidates for industrial ethanol production.
    Type: Grant
    Filed: March 30, 2016
    Date of Patent: March 3, 2020
    Inventors: Charles A Abbas, Andriy Sibirny, Kostyantyn Dmytruk, Barbara Kshanovska
  • Patent number: 10564159
    Abstract: The present invention provides methods and compositions useful in the diagnosis and management of autoimmune diseases. In particular, the present invention provides improved methods and compositions for the diagnosis and management of Graves' disease. The methods of the present invention not only avoids the need for radioactivity and are much simpler, economical, and rapid than methods traditionally used for the diagnosis of Graves' disease, but also improve upon the sensitivity and detection abilities of previous luciferase-based autoantibody detection assays. Such improvements are based upon the superior performance of assays comprising a chimeric TSH receptor in the presence of a glucocorticoid including, but not limited to, dexamethasone.
    Type: Grant
    Filed: September 26, 2017
    Date of Patent: February 18, 2020
    Assignee: Diagnostic Hybrids, Inc.
    Inventors: Leonard Kohn, James L. Brown, David R. Scholl, Yunsheng Li, Giorgio Napolitano
  • Patent number: 10557775
    Abstract: A system for generating data relating to a tissue core comprises a core needle biopsy module configured to obtain a tissue core from a locus within the body, and a tissue disintegration module operably connected to the core needle biopsy module and configured to receive a tissue core from the core needle biopsy module and convert at least a portion of the tissue core into gaseous tissue molecules. The system also comprises first vacuum pump means configured to convey a tissue core from the needle biopsy module to the tissue disintegration module, and second vacuum pump means configured to convey gaseous tissue molecules from the tissue disintegration module to an analyser module.
    Type: Grant
    Filed: April 4, 2016
    Date of Patent: February 11, 2020
    Assignee: DIOPSY LIMITED
    Inventor: Maximillian Frederick Ryan
  • Patent number: 10545133
    Abstract: Carcinomas typically invade as a cohesive multicellular unit, a process termed collective invasion. It remains unclear how different subpopulations of cancer cells contribute to this process. We developed three-dimensional (3D) organoid assays to identify the most invasive cancer cells in primary breast tumors. Collective invasion was led by specialized cancer cells that were defined by their expression of basal epithelial genes, such as cytokeratin-14 (K14) and p63. Furthermore, K14+ cells led collective invasion in the major human breast cancer subtypes. Importantly, lumenal cancer cells were observed to convert phenotypically to invasive leaders following induction of basal epithelial genes. Although only a minority of cells within lumenal tumors expressed basal epithelial genes, knockdown of either K14 or p63 was sufficient to block collective invasion. Our data reveal that heterotypic interactions between epithelial subpopulations are critical to collective invasion.
    Type: Grant
    Filed: May 13, 2014
    Date of Patent: January 28, 2020
    Assignee: The Johns Hopkins University
    Inventors: Andrew Josef Ewald, Kevin Cheung
  • Patent number: 10542883
    Abstract: A system and method for diagnosing and treating glaucoma is presented. The system imparts pressure on the anterior chamber of a eye using a coupling mechanism, while capturing three-dimensional imagery of the eye using optical coherence tomography angiography. Applying pressure in various areas of the eye, imparts changes that can help detect parts of the eye, or diagnose certain disorders related to the drainage of aqueous humor. A controller coupled to the optical coherence tomography angiography scanner may be used to guide a laser for excision of the trabecular meshwork for enhancing aqueous humor drainage thus lowering intraocular pressure and preventing glaucoma.
    Type: Grant
    Filed: August 26, 2016
    Date of Patent: January 28, 2020
    Inventors: Patrick Gooi, Kevin Warrian
  • Patent number: 10537891
    Abstract: A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.
    Type: Grant
    Filed: March 12, 2018
    Date of Patent: January 21, 2020
    Inventors: Oliver Egeler, Steven Woodside
  • Patent number: 10526380
    Abstract: A nucleic acid molecule encoding a fusion protein composed of a plant cryptochrome at the amino terminus, and a GTPase-Activating Protein SH3 Domain-Binding Protein (G3BP) is provided for light-dependent, G3BP-mediated stress granule formation.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: January 7, 2020
    Assignee: St. Jude Children's Research Hospital
    Inventors: Joseph Paul Taylor, Peipei Zhang
  • Patent number: 10501735
    Abstract: Devices for use in extracting an analyte of interest from a sample are described. In one embodiment, a device is comprised of a first plurality of chambers, where one or more chambers in the plurality of chambers has a deep end and a shallow end with a depth d1. A channel disposed between at least two adjacent chambers in the plurality of chambers has a depth greater than d1. The dimensions of the chamber and channel provide control of fluid movement in the device, particularly when introducing fluid into the device for its use and during use of the device.
    Type: Grant
    Filed: August 21, 2013
    Date of Patent: December 10, 2019
    Assignees: Quidel Corporation, Northwestern University
    Inventors: David M. Kelso, Kunal Sur, Tom Westberg, Zaheer Parpia, Mark J. Fisher
  • Patent number: 10493153
    Abstract: The invention relates to a method for photoreleasing a moiety G from a compound of the formula A-G. The methods of the present invention comprise irradiating a composition comprising the compound of the formula A-G at a wavelength of >500 nm, so as to photorelease the moiety G from the moiety A; wherein the moiety A comprises a chromophore and the moiety G comprises an organic compound.
    Type: Grant
    Filed: September 24, 2018
    Date of Patent: December 3, 2019
    Assignee: Iowa State University Research Foundation, Inc.
    Inventor: Arthur Henry Winter
  • Patent number: 10494651
    Abstract: Process are provided which are effective for controlling medium conductivity during fermentation of a CO-containing gaseous substrate while providing an STY of about 10 g ethanol/(L·day) or more. The process includes balancing medium conductivity, specific carbon uptake or cell density levels.
    Type: Grant
    Filed: November 14, 2017
    Date of Patent: December 3, 2019
    Inventors: Ryan H. Senaratne, Peter Simpson Bell, Song Liu, Syrona R. Scott
  • Patent number: 10488402
    Abstract: A computer system is provided for determining the relative effectiveness of anti-cancer drugs. The interface has selectable options, including an option to manage drug testing parameters, and enables user selection of desired drug testing parameters in relation to a virtual well plate associated with a physical well plate of a spectrophotometer. The computer system causes the spectrophotometer to start a drug test, wherein the physical well plate includes at least one test well containing viable cancer cells; and at least one drug candidate in a predetermined concentration; and at least one control well containing the viable cancer cells alone. The system records the optical density of the well at a predetermined wavelength at selected time intervals for a selected duration of time, and stores the optical density and time measurements in the database.
    Type: Grant
    Filed: October 4, 2016
    Date of Patent: November 26, 2019
    Assignee: Pierian Biosciences, LLC
    Inventors: Mathieu Perree, Allan E. Hallquist, Olivier Petit
  • Patent number: 10487311
    Abstract: Methods for expanding proliferating populations of neuronal subtype-specific progenitors and creating substantially pure populations of motor neurons are provided herein. In particular, the present invention provides methods for maintaining the unique gene profile and differentiation potential of neuronal subtype-specific progenitors, such as motor neuron progenitors and hindbrain serotonergic neural progenitors.
    Type: Grant
    Filed: October 5, 2017
    Date of Patent: November 26, 2019
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Su-Chun Zhang, Zhongwei Du
  • Patent number: 10478157
    Abstract: Methods and devices for delivering an agent to a solid tissue in vivo for assessment of efficacy are described. One method involves withdrawing of a needle from and injecting of the agent into the solid tissue; another method involves delivering the agent using a plurality of microdialysis probes to a solid tissue.
    Type: Grant
    Filed: November 28, 2016
    Date of Patent: November 19, 2019
    Inventors: Jason Frazier, Richard Klinghoffer, Marc Grenley
  • Patent number: 10472674
    Abstract: A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: November 12, 2019
    Assignee: GENAPSYS, INC.
    Inventors: Hesaam Esfandyarpour, Mark Oldham
  • Patent number: 10471427
    Abstract: A fluidic manifold cartridge includes a plurality of source fluid inlets and fluid outlets. A plurality of fluid input flow channels are provided. Each fluid inlet is in fluid communication with a fluid input flow channel. Each fluid input flow channel directs fluid from the fluid inlet past a plurality of valves. A plurality of fluid output flow channels are in fluid communication with a fluid outlets. Each valve includes a valve seat, a portion of membrane, and a control fluid opening. Each valve has an open and closed condition. The valve in the open condition directs fluid from a fluid input flow channel to a fluid output flow channel. The control fluid opening directs control fluid to move the membrane so as to change the valve between the open and closed conditions. Systems and methods for fluidic manifold are also disclosed.
    Type: Grant
    Filed: April 24, 2017
    Date of Patent: November 12, 2019
    Inventor: Felipe Echeverri
  • Patent number: 10471146
    Abstract: An object of the present invention is to provide an anticancer drug capable of treating cancer by finding a target molecule specifically expressed in cancer cells and by specifically acting on the target molecule, and to provide a cancer testing method including a step of measuring the target molecule in a sample. The present invention provides an anticancer drug containing, as an active ingredient thereof, an anti-transmembrane protein 180 (TMEM-180) antibody or an antigen-binding fragment thereof. In addition, the present invention provides a cancer testing method including a step of measuring the amount of TMEM-180 in a sample collected from a subject.
    Type: Grant
    Filed: September 8, 2015
    Date of Patent: November 12, 2019
    Assignees: National Cancer Center, RIN Institute Inc.
    Inventors: Yasuhiro Matsumura, Masahiro Yasunaga, Shinji Saijou, Shingo Hanaoka
  • Patent number: 10458997
    Abstract: A method for determining a background shift is presented. The method includes obtaining a plurality of optical measurement sets of a sample taken by a sample analyzer at different points in time. Each set includes one measurement taken in a first channel and another measurement taken in a second channel different from the first channel. The plurality of optical measurement sets includes one set group and another set group. The measurements in the one set group show a higher signal change than the measurements in the other group. A first background signal is determined based on a relation of measurements in the first and second channels in the one group. A second background signal is determined based on a relation of measurements in the first and second channels in the one group and the other group. A background shift is determined based on the first and second background signals.
    Type: Grant
    Filed: August 30, 2017
    Date of Patent: October 29, 2019
    Assignee: Roche Diagnostics Operations, Inc.
    Inventor: Rolf Knobel
  • Patent number: 10437909
    Abstract: A food safety management tool that utilizes a mathematical model based on differential equations that is generalized for describing the continuous growth-death kinetics of microbial populations in foodstuffs. The method is used to provide a way to control target microorganisms when designing product formulations of minimally processed foodstuffs or when processing foods with high pressures, temperatures, or other lethal agents to achieve effective pasteurization, disinfection, or sterilization of foodstuffs, and includes the use of model parameters to predict food formulations to inhibit the growth of microorganisms and the processing times needed to reduce microbial hazards to levels that ensure consumer safety.
    Type: Grant
    Filed: September 1, 2009
    Date of Patent: October 8, 2019
    Assignee: Brandeis University
    Inventors: Christopher J Doona, Edward W Ross, Florence E Feeherry, Kenneth Kustin
  • Patent number: 10422789
    Abstract: The present invention is related to a method for diagnosing Niemann-Pick disease in a subject comprising a step a), wherein the step a) comprises detecting a biomarker in a sample from the subject.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: September 24, 2019
    Assignee: Centogene AG
    Inventors: Arndt Rolfs, Hermann Mascher
  • Patent number: 10422748
    Abstract: The device for the microbiological analysis of a support (41) adapted to contain microorganisms marked by a fluorophore agent comprises illuminating means adapted to illuminate said support (41) comprising two bases (21) on each of which is mounted at least one group of light sources (22), with said sources (22) of that group being regularly spaced from each other to form an array for illuminating said support (41), said bases (21) being fixed to a frame of said device, one on each side of an opening (9) formed in said frame, with said bases (21) being inclined towards each other in the direction of a predetermined location for reception of said support (41) in said device.
    Type: Grant
    Filed: September 11, 2009
    Date of Patent: September 24, 2019
    Assignee: EMD Millipore Corporation
    Inventors: Gael Waiche, Sebastien Ribault
  • Patent number: 10416065
    Abstract: The present invention refers to a device for the analysis of a particle comprising an analysis chamber adapted to contain a positioning fluid. A parameter of the particle suspended in the positioning fluid is detected by means of a detection and control unit. A positioning unit, during a particle analysis operation, is activated and deactivated on the basis of the detected parameter of the particle. The detection and control unit can activate the at least one positioning unit so as to generate a temporary positioning flow in the positioning fluid, such that said temporary positioning flow acts directly on the particle and drives the position of the particle so as to move it into a predefined position in the analysis chamber. The detection and control unit can also deactivate the at least one positioning unit when the particle to be analyzed is in the predefined position, such that the positioning fluid is at rest.
    Type: Grant
    Filed: December 16, 2015
    Date of Patent: September 17, 2019
    Assignee: CELLDYNAMICS I.S.R.L.
    Inventors: Daniele Gazzola, Simone Pasqua, Emanuele Centurioni
  • Patent number: 10413940
    Abstract: A transducer laminate in which electrical contact is made between electrical conductors (C1, C2) and a transducer layer (TY). The transducer laminate includes two adhesive-coated foils (F1, F2), whose adhesive coatings (AC1, AC2) are arranged to face each other. At a first position (A-A?) along the length of the two electrical conductors (C1, C2) the two electrical conductors (C1, C2) are sandwiched between the adhesive coatings (AC1, AC2) of the two adhesive-coated foils, and the transducer layer (TY) is also sandwiched between the two electrical conductors (C1, C2) such that electrical contact is made with the electrodes (E1, E2) on the transducer layer (TY). At a second position (B-B?) along the length of the two electrical conductors (C1, C2) the two electrical conductors (C1, C2) are sandwiched between the adhesive coatings (AC1, AC2) of the two adhesive-coated foils and there is no transducer layer (TY) sandwiched between the two electrical conductors (C1, C2).
    Type: Grant
    Filed: July 21, 2016
    Date of Patent: September 17, 2019
    Inventors: Willem-Jan Arend De Wijs, Johannes Wilhelmus Weekamp
  • Patent number: 10406188
    Abstract: The present disclosure relates to a method for inhibiting telomerase in cancer cells by means of luterion and, more particularly, to a method for inhibiting telomerase activity in cancer tissues, cancer cells or a cancer patient, the method in which administered are: an anti-cancer composition comprising luterion; a composition for inhibiting telomerase activity in cancer cells; and luterion. Luterion, according to the present disclosure, has the advantage of effectively inhibiting only the proliferation of cancer cells, without affecting normal cells, by inhibiting telomerase activity in cancer cells.
    Type: Grant
    Filed: July 5, 2017
    Date of Patent: September 10, 2019
    Assignee: LUTERION CO., LTD.
    Inventors: Young Ah Kwon, Won Cheol Choi, Suk Hoon Choi, Chang Hoon Choi
  • Patent number: 10408842
    Abstract: Electrophoretic separation methods and systems for performing the same are provided. The methods and systems find use in a variety of different electrophoretic separation applications, such as sub-cellular Western blotting of single cells.
    Type: Grant
    Filed: May 29, 2015
    Date of Patent: September 10, 2019
    Assignee: The Regents of the University of California
    Inventors: Kevin A. Yamauchi, Amy E. Herr
  • Patent number: 10408934
    Abstract: An object information acquiring apparatus according to the present invention includes: a supporter which supports a plurality of conversion elements each converting a photoacoustic wave from an object into an electrical signal; an imager which acquires an image of the object; a scanner which changes a relative position between the object and the supporter; an acquirer which acquires first and second specific information of the inside of the object based on an electrical signal acquired at a first and second relative position between the object and the supporter; and a corrector which corrects a deviation between the first and the second specific information based on positional information of the object.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: September 10, 2019
    Inventor: Jiro Tateyama
  • Patent number: 10383897
    Abstract: The present invention provides placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. The invention also provides methods of differentiating the placental stem cells. The invention further provides methods of using the placental stem cells in assays and for transplanting.
    Type: Grant
    Filed: June 10, 2015
    Date of Patent: August 20, 2019
    Assignee: Celularity, Inc.
    Inventors: James Edinger, Qian Ye, Jai-Lun Wang, Robert Hariri
  • Patent number: 10379049
    Abstract: Embodiments generally relate to a system for reading fluorescent-labelled diagnostic assays for in-vitro diagnostic applications. The system comprises a receiving member adapted to receive a fluorescent-labelled diagnostic assay cartridge carrying a fluorescent-labelled diagnostic assay; at least one excitation module configured to illuminate the diagnostic assay, when the diagnostic assay cartridge is placed in the receiving member; a camera module for capturing an image of the illuminated diagnostic assay placed in the receiving member; a processor for receiving the captured image from the camera module and determining whether or not a target analyte was present in the diagnostic assay captured by the camera module; and memory storing firmware, the firmware including a brightness compensation module configured to adjust the intensity of an image of a diagnostic cartridge captured by the camera module, in order to emulate a uniform field of illumination over the diagnostic cartridge.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: August 13, 2019
    Assignee: Lumos Diagnostics IP PTY LTD
    Inventor: William Samuel Hunter
  • Patent number: 10365220
    Abstract: Raman spectra of cells, such as normal human T- and B-cells from peripheral blood or human tonsil and the corresponding transformed cells are obtained by optically trapping the cells and obtaining their Raman spectra. The trapped cells can be subjected to one, two, or more different excitation wavelengths, and each wavelength of the corresponding Raman spectra can be stored in a separate channel. In preferred embodiments, two spectra are subtracted from each other to give a difference spectrum and each channel is analyzed independently to characterize the trapped cell. Alternatively, the Raman spectrum can be subjected to Principal Component Analysis (PCA) in order to characterize the trapped cell. The trapped cell thus classified can be sorted, or further manipulated.
    Type: Grant
    Filed: May 31, 2006
    Date of Patent: July 30, 2019
    Inventors: James W. Chan, Thomas R. Huser, Stephen M. Lane, John C. Rutledge, Douglas S. Taylor, Theodore Zwerdling, Ronald Li
  • Patent number: 10364473
    Abstract: Methods of diagnosing and treating patients afflicted with acne, including diagnosing one as having acne if the individual possesses RT4, RT5, RT7, RT8, RT9, or RT10. Methods for treating acne include administering an effective amount of a drug specifically targeting RT4, RT5, RT7, RT8, RT9, or RT10, such as small molecules, antisense molecules, siRNAs, biologics, antibodies, phages, vaccines, or combination thereof.
    Type: Grant
    Filed: September 6, 2016
    Date of Patent: July 30, 2019
    Inventors: Huiying Li, Shuta Tomida, Robert L. Modlin, Jeffery F. Miller, Sorel T. Fitz-Gibbon
  • Patent number: 10357772
    Abstract: Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.
    Type: Grant
    Filed: August 20, 2018
    Date of Patent: July 23, 2019
    Assignees: President and Fellows of Harvard College, Brandeis University
    Inventors: Seth Fraden, Hakim Boukellal, Yanwei Jia, Seila Selimovic, Amy Rowat, Jeremy Agresti, David A. Weitz
  • Patent number: 10314943
    Abstract: The present invention refers to a process, and to the related apparatus, for the in vitro preparation of a biomimetic tissue prostheses of the tympanic membrane from mesenchymal stem cells; such prostheses are used for repairing or reconstructing the injured tympanic membrane in patients needing it, for example as a consequence of various trauma or pathologies.
    Type: Grant
    Filed: September 17, 2014
    Date of Patent: June 11, 2019
    Assignee: Azienda Ospedaliero-Universitaria Pisana
    Inventors: Serena Danti, Stefano Berrettini, Stefano Marrazza, Cesare Stefanini