Abstract: A gas dissolved liquid manufacturing device includes: a pump configured to pressurize a liquid; a pipe communicating with the pump; a nozzle disposed in the pipe, the nozzle being configured to generate micro bubbles using a supplied gas; and a gas-liquid separation tank whose upper part communicates with the pipe, the gas-liquid separation tank being configured to separate a gas-liquid mixture generated by the nozzle into a gas and a liquid.

Abstract: Disclosed is a jacketed, tiered baffle, bioreactor tank comprising an outer cylindrical-shaped jacket and a cylindrical tank having an inner tank surface defining a chamber configured for supporting a flexible bag disposed within the chamber, and an outer tank surface having tiered baffles configured for routing a heat exchange fluid around the entirety of the outer tank surface, the cylindrical tank disposed axially within the outer cylindrical-shaped jacket. The outer cylindrical-shaped jacket is sealed to the cylindrical tank in a manner sufficient to prevent or minimize loss of the heat exchange fluid.

Abstract: A reactor system is provided for improved fermentation of a gaseous substrate through the introduction of a secondary loop to a forced-circulation loop reactor. The reactor comprises a primary loop through which fermentation broth comprising a gaseous substrate is circulated through a riser segment and a downcomer section by a loop pump. Downstream of the loop pump a portion of fermentation broth is withdrawn from the downcomer section and is directed to the top of the reactor via a secondary loop. Further provided is a method for improving the mass transfer of a gaseous substrate to a fermentation broth in a fermentation vessel comprising a secondary loop. Further provided is a method for reducing foam in the headspace of a fermentation vessel comprising a secondary loop.

Abstract: Provided is a defoamer for fermentation which has excellent dispersibility in water and forms neither a precipitate nor oil droplets when the dispersion is heated, and which is highly effective in defoaming fermentation media. This defoamer contains a reaction product obtained by mixing a fat or oil having an iodine value of 40 to 130 with glycerin or like in a molar ratio of from 3/2 to 1/2 to obtain a mixture, causing 4 to 17 mol of propylene oxide to add to 1 mol of the mixture, and then causing 20 to 40 mol of ethylene oxide and 70 to 110 mol of propylene oxide to block-wise add thereto in this order, the reaction product having an ethylene oxide/propylene oxide molar ratio of from 1/4 to 2/5.

Abstract: The present invention concerns in one aspect a separator for separating the gas and liquid phases of a foam and, in another aspect, a foam reducing apparatus. The separator comprises a first side and a second side and having through-flow means provided therein for permitting a foam or a foam phase to pass from the first side to the second side, the separator further comprising at least one foam contacting surface having a low surface energy, and means for recovering at least one separated foam phase from the foam. The foam reducing apparatus comprises a low surface energy material and means for contacting foam, when said foam is input to the foam reduction apparatus, along a surface of said low surface energy material. The separator and the foam reducing apparatus may be used independently or in combination to good effect to more efficiently disrupt foam to provide separate gas and liquid phases.

Abstract: A portable, self-contained incubation apparatus has a cell container in which a cell culture can grow. The cell container resides on a base along with other components that maintain the environmental conditions inside of the container within a desired range. The self-contained incubation apparatus is portable allowing the conditions within the cell container to be precisely controlled even as it is being moved from one location to another. Further, at least one transparent surface of the cell container enables observation of the cell culture. Thus, the culture can be observed while the environmental conditions within the container are being controlled by components of the incubation apparatus. Since the cells can be observed without breaking the air-tight seal of the container, observation of the cells can be performed as often as is desired without introducing contaminants to the culture or otherwise significantly affecting the growth environment within the container.

Abstract: A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

Abstract: An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.

Abstract: An object of the present invention is to provide a cell culture vessel which is simple in structure and easy to handle, and is capable of preventing damage to the cells when separated, promoting transport of nutrients and excretion of effete matter, and elevating the culturing efficiency improving effect by the structural features. In order to attain the above object, there is provided a cell culture vessel including a culture section provided with a plurality of projections having an equivalent diameter smaller than the cells to be cultured and the culture section side walls enclosing the culture section, wherein the distance between an arbitrary position on the culture section/side wall boundary line and the nearest projection is smaller than the diameter of the cells to be cultured. The effect of the projections in the vessel given to the cultured cells is enhanced.

Abstract: A device for minimizing the formation of bubbles or foam in cell culture is disclosed. The device has a manifold which directs the inflow of cells and cell culture media into a cell culture vessel so as to allow for displaced air or gas to vent from the cell culture vessel without mixing with the incoming cell culture media, thereby preventing the mixing of air and cell culture media and minimizing the formation of bubbles or foam inside the cell culture vessel.

Abstract: A bioremediation assembly and method for bioremediation are described and which includes a bioremediation reservoir defining a cavity; a pump mounted within the bioremediation reservoir and which removes the aqueous bioremediating fluid from the bioremediation reservoir; and a fluid dispensing manifold positioned within the bioremediation reservoir and which is coupled in fluid flowing relation relative to the pump, and which directs a stream of bioremediating fluid downwardly so as to aerate the volume of bioremediating fluid.

Abstract: An apparatus for scum removal is disclosed wherein toothed paddle induces turbulent effect on the fluids present in the reactor by means of its rotary motion. This results in both breaking and radial displacement of the scum, accumulated on the fluid during the course of the biochemical or chemical reaction, towards the outlet of the reactor, thereby ensuring that the reaction continues in scum free environment uninterruptedly.

Abstract: There is disclosed a defoaming rotor capable of skimming foam from the surface of the fermentation broth in a bioreactor, separating the solid and liquid components from the gaseous component of the foam, and returning the solid and liquid components to the broth.

Abstract: There is disclosed a defoaming rotor capable of skimming foam from the surface of the fermentation broth in a bioreactor, separating the solid and liquid components from the gaseous component of the foam, and returning the solid and liquid components to the broth.

Abstract: A foam detecting sensor (7) and an ultrasonic oscillation horn (13) are mounted on a vertical pipeline between a culture tank (1) and a liquid-vapor separating device (5). When overflowed foams are subjected to ultrasonic irradiation, they burst and lose volume. The burst foams have high apparent density as compared to the foams before irradiation. Thus, the concurrent use of ultrasonic oscillation devices (12, 13) and the liquid-vapor separating device (5), such as cyclone, leads to an enhanced liquid-vapor separation efficiency and higher productivity by fermentation.

Abstract: A foam detecting sensor (7) and an ultrasonic oscillation horn (13) are mounted on a vertical pipeline between a culture tank (1) and a liquid-vapor separating device (5). When overflowed foams are subjected to ultrasonic irradiation, they burst and lose volume. The burst foams have high apparent density as compared to the foams before irradiation. Thus, the concurrent use of ultrasonic oscillation devices (12, 13) and the liquid-vapor separating device (5), such as cyclone, leads to an enhanced liquid-vapor separation efficiency and higher productivity by fermentation.

Abstract: The need to filter gas emanating from a containment vessel is reduced or eliminated by maintaining, within the vessel, a gradient that concentrates most of the gaseous contaminants away from the vessel exits. This is achieved by reducing the density of gas drawn from the region near its source and discharging the reduced-density gas to another region of the vessel. Owing to its reduced density, the discharged gas already has a lower contaminant concentration than the source gas. The concentration decreases further as convection draws the discharged gas back to its source. So long as the gas flows within the containment vessel are non-turbulent, the concentration gradient remains substantially static, and the vents remain exposed only to low concentrations of contaminants. The gas density may be reduced by heating and humidification, catalytic reaction, or reaction among constituents of the gas.