Incubator Patents (Class 435/303.1)
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Patent number: 12180455Abstract: This culture device comprises: a duct having an inner wall surface which faces a culture space, and a mounting surface which faces the portion to be mounted to of the inner surface, the mounting surface being mounted to the portion to be mounted to, and an air passage being formed with the inner wall surface; and a microbial light source provided within the passage. A protrusion which comes into close contact with the mounting surface, and which protrudes towards the mounting surface, is formed on the portion to be mounted to, or a protrusion which comes into close contact with the portion to be mounted to and which protrudes towards the portion to be mounted to is formed on the mounting surface.Type: GrantFiled: March 1, 2021Date of Patent: December 31, 2024Assignee: PHC CORPORATIONInventors: Hikaru Aoki, Hiroki Hirai, Manami Baba
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Patent number: 12178074Abstract: A display device includes a display panel, a mold frame, and graphite sheets. The mold frame includes a mold slot portion therethrough to support the display panel. The graphite sheets include a central portion on a rear surface of the display panel, and end portions passing through the mold slot portion to be on the rear surface of the mold frame. The graphite sheet is rolled and arranged in a mounting space between the display panel and a back cover, such that heat generated from the display panel may be dissipated to the outside via the graphite sheet and the back cover.Type: GrantFiled: December 10, 2021Date of Patent: December 24, 2024Assignee: Samsung Display Co., Ltd.Inventors: Jonghyeon Choi, Sungwon Kim, Seokhyun Nam, Haengwon Park, Il Hyun Yang, Daehwan Jang
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Patent number: 12171950Abstract: A music matching application system with use of AGEs and a method thereof, using a sensor unit of a wearable device to detect a first parameter value of a skin surface, followed by using a computation processing unit to perform computation process based on the first parameter value in order to obtain an electric signal and to convert into a corresponding concentration value of the AGEs. In addition, a music unit is driven to correspondingly play one of the first music menus according to the concentration value, and different music program can be provided according to the concentration level, and a second music menu is further played according to a concentration change amount corresponding to a time interval. Consequently, the effect of the music program can be enhanced.Type: GrantFiled: September 23, 2021Date of Patent: December 24, 2024Assignee: NATIONAL CHENG KUNG UNIVERSITYInventor: Chen-Hsun Weng
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Patent number: 12076725Abstract: Fluidic device includes a manifold body having first and second body sides. The first body side has receiving ports forming a port array that defines a reaction region. The second body side has open-sided recesses forming reaction chambers when the fluidic device is mounted onto a sample substrate. The reaction chambers form a chamber array that defines a fluid-delivery region. The reaction region is greater than the fluid-delivery region. The manifold body also includes vent openings that open to an exterior. The fluidic device also includes upstream channels extending through the manifold body. Each of the upstream channels fluidly couples a corresponding receiving port of the port array to a corresponding reaction chamber of the chamber array. The fluidic device also includes venting channels extending through the manifold body. Each of the venting channels fluidly couples a corresponding reaction chamber of the chamber array to a corresponding vent opening.Type: GrantFiled: April 29, 2021Date of Patent: September 3, 2024Assignee: Illumina, Inc.Inventor: Darryl Donovan
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Patent number: 12064772Abstract: A chemical and/or biochemical apparatus (10) includes a thermal mount (14) having wells (26) for receiving reaction vessels (12), a thermal module (16) having a first side thermally coupled to the thermal mount (14), a first heat sink (18) thermally coupled to a second side of the thermal module, the heat sink (18) having a body and thermally conductive fins (32) extending outwards from the body of the first heat sink (18), and a printed circuit board (54) having electronic components for controlling at least the thermal module (16), an excitation light source (62), and a light sensor (52). A first set of light waveguides (60) delivers excitation light to a reaction vessel, and a second set of light waveguides (38) receives light from a reaction vessel and delivers the light to the light sensor (52). The printed circuit board (54), the excitation light source (62), the light sensor (52) and the light waveguides (38, 60) are arranged within an interior space (5) of the first heat sink (18).Type: GrantFiled: February 15, 2021Date of Patent: August 20, 2024Assignee: IT-IS International LimitedInventors: James Richard Howell, Benjamin Masterman Webster
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Patent number: 12036550Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.Type: GrantFiled: February 19, 2021Date of Patent: July 16, 2024Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Jered Haun, Xiaolong Qiu, Elliot Hui, Amrith Karunaratne, Erik Werner
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Patent number: 11952618Abstract: This invention provides biochip cartridges and instrument devices for the detection and/or analysis of target analytes from patient samples.Type: GrantFiled: January 8, 2021Date of Patent: April 9, 2024Assignee: ROCHE MOLECULAR SYSTEMS, INC.Inventors: Jon Faiz Kayyem, Jayashankar Srinivasan, Sean Ford
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Patent number: 11922800Abstract: A test system includes a testing base including a plurality of testing base containers, and a plurality of electrodes integrated into the plurality of testing base containers. The test system further includes a plurality of drive-sense circuits coupled to the plurality of electrodes, where, when enabled, the plurality of drive-sense circuits detect changes in electrical characteristics of the plurality of electrodes. The test system further includes a processing module operably coupled to receive, from the drive-sense circuits, changes in the electrical characteristics of the plurality of electrodes, and interpret the changes in the electrical characteristics of the plurality of electrodes as impedance values representative of electrical characteristics of biological material present in the test container. The test system further includes a communication module operably coupled to communicate the electrical characteristics of the biological material.Type: GrantFiled: April 29, 2021Date of Patent: March 5, 2024Assignee: SIGMASENSE, LLC.Inventors: Daniel Keith Van Ostrand, Richard Stuart Seger, Jr., Gerald Dale Morrison, Patrick Troy Gray, Phuong Huynh, Timothy W. Markison, Patricia M. Healy
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Patent number: 11892462Abstract: Devices and methods for sampling, detecting and/or characterizing particles, for example, via collection, growth and analysis of viable biological particles such as microorganisms. Devices and methods of the invention include particle samplers and impactors including a sampling head comprising one or more intake apertures, a selectively removable cover, an impactor base connected to the sampling head, and one or more magnets fixed to the sampling head, the selectively removable cover and/or the impactor base. The one or more magnets allow for robotic manipulation of the impactor devices.Type: GrantFiled: January 19, 2021Date of Patent: February 6, 2024Assignees: Pharma Integration SRL, Particle Measuring Systems, Inc.Inventors: Giovanni Scialò, Davide Recchia, Claudio Bechini
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Patent number: 11872550Abstract: A safety cabinet includes a work space where work is performed; a front panel disposed in a front surface of the work space; and an air cleaner that cleans air to be supplied to the work space. An imaging device is disposed on one side surface side of side surfaces of the work space, and a connection opening is disposed on the other side surface side facing one side surface.Type: GrantFiled: June 29, 2020Date of Patent: January 16, 2024Assignee: Hitachi Industrial Equipment Systems Co., Ltd.Inventors: Takeshi Kaneko, Takeshi Matsumura
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Patent number: 11814614Abstract: A cell culture device includes a control unit that includes a first gas supply source, a second gas supply source, and a decompression source, and a culture unit that includes a first container, a second container, a first gas channel that connects a third container, the first gas supply source, the second container to a connection point, a second gas channel, a third gas channel, a first liquid channel, a second liquid channel, a gas-liquid channel, a first valve, a second valve, a third valve, a fourth valve, and a fifth valve.Type: GrantFiled: November 2, 2020Date of Patent: November 14, 2023Assignee: Hitachi, Ltd.Inventors: Masaharu Kiyama, Midori Kato
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Patent number: 11795427Abstract: A culture vessel that can suppress foaming in a culture medium when a common nozzle is used to supply a liquid culture medium and a gas together. This culture vessel includes: a vessel body having an opening portion that communicates with a housing space; a lid that closes the opening portion; and a nozzle that passes through the and extends to the housing space, and supplies a liquid culture medium and a gas together. The nozzle has a vent opening portion that is formed so as to open in a side of the nozzle and serves as a movement path of the gas from inside of the nozzle into the housing space.Type: GrantFiled: March 22, 2019Date of Patent: October 24, 2023Assignee: Sumitomo Bakelite Co., Ltd.Inventors: Ryohei Tsukada, Toru Yakabe, Haruo Okubo, Masaharu Kiyama, Midori Kato, Hikaru Saito
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Patent number: 11768158Abstract: An implement analyzing device that is sized for receiving, more than one fluid retainer cartridge assembly is disclosed. The implement analyzing device includes a support member, a housing, a cartridge receiver, at least one cartridge heater, an Imaging, device and an implement analyzing device integrated circuit. The cartridge receiver is disposed within a cavity in the implement analyzing device and defines at least one cartridge viewing window. At least one cartridge heater disposed within the cavity and connected to the cartridge receiver. The imaging device is disposed within the cavity and arranged opposite the at least one cartridge viewing window. The implement analyzing device integrated circuit is communicatively coupled to the at least one cartridge heater and the imaging device.Type: GrantFiled: October 7, 2020Date of Patent: September 26, 2023Assignee: NEOGEN CORPORATIONInventors: Frank Eric Klein, Andrew John Wheeler, Andrew John DenHartigh, William Hoerner, Nathaniel Banner, Damon Borich, Andrea Grbavac, Alejandro Silveyra, Michelle Silveyra, Zwckxally Obregon
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Patent number: 11732242Abstract: The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells.Type: GrantFiled: March 5, 2021Date of Patent: August 22, 2023Assignee: New York Stem Cell Foundation, Inc.Inventors: Scott Noggle, Kevin C. Eggan, Stephen Chang, Susan L. Solomon
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Patent number: 11732315Abstract: Compositions and methods are described that are directed to specific and sensitive methods of target nucleic acid detection and more specifically detecting target nucleic acids directly from biological samples. The compositions and methods were developed to be easy to use involving a minimum number of steps and giving rapid and consistent results either at point of care or in high throughput situations. The compositions and methods are directed to labelled probes and their uses in Loop-Mediated Isothermal Amplification (LAMP) diagnostic tests to detect target DNA from the environment or from an individual and also to detect specific variants of the target DNA, both with similar sensitivity.Type: GrantFiled: September 28, 2022Date of Patent: August 22, 2023Assignee: New England Biolabs, Inc.Inventors: Nathan Tanner, Yinhua Zhang, Eric Hunt, Gregory Patton, Guoping Ren, Zhiru Li, Andrew Barry, Nicole Nichols, Catherine B. Poole, Harriet M. Strimpel, Ivan R. Correa, Jr., Clotilde Carlow, Esta Slayton, Thomas C. Evans, Jr.
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Patent number: 11684916Abstract: A chip for polymerase chain reaction, a method of operating a chip for polymerase chain reaction, and a reaction device are provided. The chip includes: a sample adding region, a mixing region, a temperature cycling region in a sequential arrangement, and at least one driving unit group. The at least one driving unit group includes a plurality of driving units and is configured to drive a liquid drop to move and sequentially pass through the sample adding region, the mixing region, and the temperature cycling region.Type: GrantFiled: January 9, 2019Date of Patent: June 27, 2023Assignees: BEIJING BOE OPTOELECTRONICS TECHNOLOGY CO., LTD., BOE TECHNOLOGY GROUP CO., LTD.Inventors: Wenliang Yao, Nan Zhao, Haochen Cui, Peizhi Cai, Fengchun Pang, Yue Geng, Le Gu, Yuelei Xiao, Hui Liao, Yingying Zhao, Chuncheng Che
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Patent number: 11679388Abstract: A cartridge for delivering a payload to cells of a cell suspension is provided, wherein the cartridge comprises an input channel that delivers the cell suspension to a first plurality of branch channels, and wherein the first plurality of branch channels each deliver the cell suspension into a respective one or a plurality of microfluidic chips or filters. Cell suspension exiting a microfluidic chip or filter flows into a respective one of a second plurality of branch channels, and is then delivered to an output channel by which it exits the cartridge. The cartridge may comprise a plurality of removable covers that hold the chips or filters in place against a body of the cartridge in which the input channel, output channel, and branch channels are formed.Type: GrantFiled: April 6, 2020Date of Patent: June 20, 2023Assignee: SQZ Biotechnologies CompanyInventor: Maisam Dadgar
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Patent number: 11608521Abstract: The object of the invention is a method of detecting genetic material in a biological sample in which the biological sample is loaded into the reaction cartridge (6) and then the reaction cartridge (6) is placed in the control device, the collected biological sample is taken to the isolation chamber (7), isolation of biological material from the tested sample by heating the isolation chamber (7), the isolated genetic material is moved into a plurality of reaction chambers (8.1, 8.2, 8.3, 8.4), genetic material is amplified by heating the reaction chambers (8.1, 8.2, 8.3, 8.4), lyophilized reagents for genetic material amplification together with lyophilized fluorescent tag intercalating with genetic material are present in the reaction chambers (8.1, 8.2, 8.3, 8.4), and signal detection from fluorescent tags is carried out along with the genetic material amplification stage.Type: GrantFiled: August 25, 2020Date of Patent: March 21, 2023Assignee: Genomtec S.A.Inventors: Miron Tokarski, Henryk Waldemar Roguszczak
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Patent number: 11592381Abstract: A system and method for high-throughput, high-resolution gas sorption screening are provided. An example system includes a sample chamber with a hermetic seal and a heat exchanger system. The heat exchanger system includes a heat exchanger disposed in the sample chamber, a coolant circulator fluidically coupled to the heat exchanger, and a sample plate comprising sample wells in contact with the cooling fluid from the coolant circulator. The system also includes a gas delivery system. The gas delivery system includes a gas source and a flow regulator. A temperature measurement system is configured to sense the temperature of the sample wells.Type: GrantFiled: January 29, 2021Date of Patent: February 28, 2023Assignee: Saudi Arabian Oil CompanyInventors: Mohammad G. Alhashim, Mohammed Alshammasi
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Patent number: 11441115Abstract: When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.Type: GrantFiled: March 12, 2019Date of Patent: September 13, 2022Assignees: DAI NIPPON PRINTING CO., LTD., OSAKA UNIVERSITY, TERUMO KABUSHIKI KAISHAInventors: Taro Nagai, Yoshiki Sawa, Shigeru Miyagawa, Kenji Oyama
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Patent number: 11430927Abstract: A semiconductor light-emitting device includes a semiconductor light-emitting element that emits ultraviolet radiation at a wavelength of not more than 360 nm, a package substrate that houses the semiconductor light-emitting element, a thin film layer that is formed on the package substrate and has a predetermined thickness, and a sealing material made of a silicone resin which is provided on the thin film layer so as to have a lens shape and seals the semiconductor light-emitting element, in which the sealing material forms a contact angle of not less than 15° with the thin film layer.Type: GrantFiled: April 10, 2020Date of Patent: August 30, 2022Assignee: Nikkiso Co., Ltd.Inventors: Shuichiro Yamamoto, Hiroyasu Ichinokura
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Patent number: 11377653Abstract: The invention relates to a method for extracting DNA, comprising the steps of: providing (400) a lysate (11) in a sample vessel (1), feeding (500) an DNA absorbing substance (15) to the sample vessel (1), closing (700) the sample vessel (1) by means of a wash filter element (3), feeding (600) a wash fluid (12) to the sample vessel (1) through the wash filter element (3), and discharging (700) the wash fluid (12) from the sample vessel (1) through the wash filter element (3). The DNA absorbing substance can be retained from the wash filter element (3).Type: GrantFiled: November 8, 2017Date of Patent: July 5, 2022Assignee: FĂĽll Process SAInventors: Joel Johannes Frank, Thomas Brinz
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Patent number: 11351553Abstract: Provided is a heating mechanism for a biochemical reaction device, including: a heat-conducting body including: at least one accommodating groove each including a chamber and an opening communicating with the chamber; a clamping hole, in communication with the opening and for inserting a reaction tube; and at least one heat-conducting block, movably disposed in the chamber and having one end connected with an elastic element and another opposite end provided with an abutting portion, the elastic element enabling the abutting portion of the heat-conducting block to protrude from the opening and locate in the clamping hole; and a temperature control element connected to the heat-conducting body for heating and regulating a temperature of the heat-conducting block.Type: GrantFiled: September 19, 2017Date of Patent: June 7, 2022Assignee: GENEREACH BIOTECHNOLOGY CORP.Inventors: Chun-Ming Lee, Ching-Ko Lin, Yun-Lung Tsai, Pei-Yu Lee, Chen Su, Hsiao-Fen Chang, Fu-Chun Li
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Patent number: 11344886Abstract: A heater for a microfluidic test card is disclosed herein. In a general example embodiment, a test card for analyzing a fluid sample includes at least one substrate layer including a microchannel extending through at least a portion of one of the substrate layers, and a printed substrate layer that is bonded to or printed on one substrate layer of the at least one substrate layer. The printed substrate layer includes a heater printed on the printed substrate layer so as to align with at least a portion of the microchannel. The heater includes two electrodes aligned on opposite sides of the microchannel, and a plurality of heater bars electrically connecting the two electrodes. The plurality of heater bars includes a central heater bar disposed between outer heater bars.Type: GrantFiled: January 24, 2020Date of Patent: May 31, 2022Assignee: FluxErgy, LLCInventors: Ashwin Raghunathan, Steve Lee, Ryan Alan Revilla, Tej Rushikesh Patel
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Patent number: 11338296Abstract: There is described a variable-temperature reactor for hosting a predetermined reaction therein. The reactor comprises a reaction cell, a heater, and a heat sink. The reaction cell has a reaction volume with thickness Hv and width Wv where Wv>4Hv and is defined by faces with one of the larger area faces of the reaction volume being bounded by an outer wall with thickness Hw. The heater is in contact with the said outer wall. The heater comprises a heat-generating heater element located on the face closer to the reaction volume and a heater support on the opposite face. The heater support is in contact with a heat sink, such that the heater support provides a thermal resistance RT between the heater element and the heat sink. The reactor, when filled with reagents having thermal diffusion coefficient Dv has a diffusion time tv, in the thickness direction, tv=Hv2/Dv. tv is less than the reaction time constant tR.Type: GrantFiled: May 19, 2021Date of Patent: May 24, 2022Assignee: Lex Diagnostics Ltd.Inventors: Justin Buckland, Tom Jellicoe, Alex Stokoe, Amaru Araya-Williams
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Patent number: 11299702Abstract: The present invention is directed to compositions, tools, methods and devices to culture microorganisms and, in particular, to compositions, tools, methods and devices for the detection of microorganisms in biological samples.Type: GrantFiled: June 11, 2018Date of Patent: April 12, 2022Assignee: Vax-Immune, LLCInventors: Bhairavi Parikh, James Stone
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Patent number: 11193099Abstract: Provided is a highly reliable temperature adjustment apparatus which uses a temperature adjustment element and quantitatively evaluates the temperature adjustment performance of the temperature adjustment element. The temperature adjustment apparatus is provided with: a temperature adjustment element; one or more temperature detection elements provided near the temperature adjustment element; a calculation unit for calculating the output of the temperature detection element; and a display unit for displaying at least one of a time calculated by the calculation unit as a time at which the temperature adjustment performance of the temperature adjustment is predicted to be below a desired level, the number of operations and the time of current conduction or a warning based on the result of calculation.Type: GrantFiled: July 27, 2016Date of Patent: December 7, 2021Assignee: HITACHI HIGH-TECH CORPORATIONInventors: Wataru Sato, Nobuyuki Isoshima, Kohshi Maeda, Daisuke Morishima
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Patent number: 11029504Abstract: Disclosed are a window apparatus for obtaining a microscopic image of in vivo breast tissue and a method for using the window apparatus to obtain cell-level and molecular-level microscopic images of in vivo breast tissue while maintaining the biological environment of the in vivo breast tissue. In one embodiment, a window apparatus comprises a first chamber having a ring structure with an open window on the center, and a cover glass disposed on the upper part and breast tissue placed on the lower part; a second chamber having an opening window on the center coupled to the first chamber to support the breast tissue; and a chamber holder for fixing the first and second chambers having a tilting mount placing unit with the tilting mount placed to have the cover glass and an object lens of a confocal microscope system stay parallel to each other.Type: GrantFiled: August 31, 2016Date of Patent: June 8, 2021Assignees: MEDICINAL BIOCONVERGENCE RESEARCH CENTER, KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Pil Han Kim, Yeseul Kim, Inwon Park, Sunghoon Kim
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Patent number: 10967379Abstract: An incubator stand for holding a plurality of incubators in a stacked arrangement, the incubator stand comprising: a stand base; a shaft element upstanding from the stand base; and a plurality of incubator bases rotatably received on the shaft element, each incubator base forming a support for or forming part of an incubator. A stop element is also provided which prevents or limits angular displacement of each incubator base, so that each incubator base can be pivoted out from a stack of said incubator bases to in use access an incubator associated therewith without causing the stack to topple. An incubator docking system and incubator system for transferring incubators between different environmental conditions are also provided.Type: GrantFiled: September 21, 2017Date of Patent: April 6, 2021Assignee: Planer LimitedInventors: Stephen James Butler, Stephen Mark Joseph Wilkins
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Patent number: 10266801Abstract: According to the present invention, a simple structure can be used to achieve reliable liquid delivery with no residual air, and simple attachment/detachment of a culture vessel, and thus a closed-system cell culture device exhibiting high reliability can be constructed at low cost. In the present invention, a liquid is supplied or discharged while a culture vessel is in an inclined state. The culture vessel is provided with two flow paths, namely an intake flow path and a discharge flow path, which connect a culture chamber and a connection member. Points where the flow paths join with the culture chamber are respectively configured as an intake port and a discharge port. The discharge port is provided in the position nearest to the axis of inclination of the culture vessel. The intake port is provided in a plane projected from a vertical plane including the aforementioned axis of inclination.Type: GrantFiled: December 24, 2014Date of Patent: April 23, 2019Assignee: Hitachi High-Technologies CorporationInventors: Akihiro Shimase, Kazumichi Imai, Eiichiro Takada, Sadamitsu Aso
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Patent number: 10087410Abstract: In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610, and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.Type: GrantFiled: March 25, 2013Date of Patent: October 2, 2018Assignee: Hitachi, Ltd.Inventors: Takayuki Nozaki, Guangbin Zhou, Masaharu Kiyama, Taku Nakamura, Shizu Takeda, Ryota Nakajima, Masakazu Sugaya, Koichi Terada
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Patent number: 10023834Abstract: A culture apparatus that cultivates a culture, which includes an outer case, an inner case configured with metal plates inside the outer case, a heater outside the inner case, and a door, which opens/closes an opening formed in front faces of the inner cases, includes: shelf rests on which side parts of a bottom plate of a shelf are to be placed, the shelf rests formed by pressing on side plates of the inner case; and a heat transfer sheet attached to an outer surface of at least one of the side plates and configured to transfer heat of the heater to the inner case, the heat transfer sheet including slits, intermittently formed, to bend the sheet to depressions of the outer surface of the side plate at positions corresponding to the shelf rests, after the heat transfer sheet is attached to the outer surface.Type: GrantFiled: March 9, 2016Date of Patent: July 17, 2018Assignee: PHC Holdings CorporationInventor: Tomoyoshi Tokumaru
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Patent number: 9927605Abstract: A certain material irregularly expressed in an observation area is effectively observed. An observing apparatus includes a first observing unit performing a time lapse shooting of a predetermined observation area, a first discriminating unit discriminating whether or not a first material is expressed in the observation area based on an image obtained by the first observing unit, and a second observing unit starting a time lapse shooting relating to a part where the first material is expressed at a timing when the first material is expressed in the observation area, in which a shooting frequency of the time lapse shooting by the second observing unit is higher than a shooting frequency of the time lapse shooting by the first observing unit.Type: GrantFiled: August 2, 2012Date of Patent: March 27, 2018Assignee: NIKON CORPORATIONInventor: Hiroaki Kii
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Patent number: 9803168Abstract: An incubator 1 includes a cultivating chamber 4 for cultivating culture in a plurality of containers 3 and a supply device 5 provided outside the cultivating chamber 4 and supplying steam into the cultivating chamber 4 for humidification. The supply device 5 includes a supply chamber 5A in which a tray 42 for reserving water is accommodated and an ultrasonic atomizing device 43 for atomizing water. The water in the tray 42 is easily made into steam by the ultrasonic atomizing device 43, passes through an HEPA filter 45 provided in an opening portion 5B and is supplied into the circulation passage 37 of the cultivating chamber 4.Type: GrantFiled: January 25, 2016Date of Patent: October 31, 2017Assignee: SHIBUYA CORPORATIONInventors: Tetsuya Nishimura, Masaomi Shioya, Katsuki Hashimoto
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Patent number: 9789459Abstract: A nucleic acid amplification reaction vessel includes a first inner wall, and a second inner wall that is arranged opposite to the first inner wall, in which a distance between the first inner wall and the second inner wall is a length in which a nucleic acid amplification reaction solution comes into contact with both the first inner wall and the second inner wall when the nucleic acid amplification reaction solution is poured.Type: GrantFiled: February 18, 2015Date of Patent: October 17, 2017Assignee: Seiko Epson CorporationInventor: Toshiro Murayama
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Patent number: 9657259Abstract: The present invention relates to a cell culture flask for culturing a cell using a culture solution, particularly to a cell culture flask for culturing a cell without any contamination, to automatically introduce and discharge the culture solution or gases and to be stacked in turn, and a cell culture device having the same. The present invention provides a cell culture flask comprising a culture space; one or more culture solution inlet ports; one or more culture solution outlet ports; and one or more gas inlet ports, wherein the cell culture flask is made airtight and formed of a transparent material so that a user can see an internal portion of the cell culture flask, and a cell culture device having the same.Type: GrantFiled: May 1, 2015Date of Patent: May 23, 2017Assignee: Corestem Co., Ltd.Inventors: Kyung Suk Kim, Jai Jun Choung
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Patent number: 9617508Abstract: A culture container includes a first transparent member being capable of keeping a predetermined temperature; a second transparent member facing to the first transparent member; a housing member to which the first transparent member and the second transparent member are adhered forming a culture space being capable of housing a well plate together with the first transparent member and the second transparent member; and a sealing member for sealing a liquid injected into the culture space between the first transparent member and the housing member.Type: GrantFiled: September 16, 2013Date of Patent: April 11, 2017Assignee: Sony CorporationInventors: Eiichi Tanaka, Shinsuke Haga, Shin Hasegawa, Tatsuya Minakawa, Suguru Dowaki, Eriko Matsui
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Patent number: 9588069Abstract: The present disclosure provides apparatus, systems, and methods for conducting rapid, accurate, and consistent heated amplifications and/or thermal melt analyses.Type: GrantFiled: July 31, 2013Date of Patent: March 7, 2017Assignee: GEN-PROBE INCORPORATEDInventors: David Opalsky, Norbert D. Hagen, Rolf Silbert, Sean Siyao Chiu, Haitao Li
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Patent number: 9579657Abstract: This disclosure provides a thermocycler system for performing polymerase chain reaction. The thermocycler system can comprise a plurality of bus bars, a microplate, a clamp and a current application device in electrical communication with the bus bars. The clamp is suitable for forming a low resistance electrical connection between the microplate and an individual bus bar of the plurality of bus bars. In some instances, the clamp includes a spring-loaded pivot and a ram. Operation of the clamp forces the microplate into contact with the bus bar, or vice versa, to provide an electrical contact between the microplate and the bus bar. The microplate can comprise a substrate having a metallic material for heating samples, and a barrier layer disposed adjacent to the substrate.Type: GrantFiled: May 23, 2013Date of Patent: February 28, 2017Assignee: BJS IP LTDInventors: Nicholas Burroughs, Richard Lewis, Ian Gunter
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Patent number: 9558912Abstract: The present invention aims at providing an ion milling apparatus for emitting an ion beam to a sample to process the sample and capable of controlling the temperature of the sample with high accuracy regardless of deformation or the like of the sample being irradiated with the ion beam, and proposes an ion milling apparatus including at least one of a shield holding member for supporting a shield for shielding the sample from the ion beam while exposing a part of the sample to the ion beam; a shifting mechanism for shifting a surface of the sample stand in contact with the sample following deformation of the sample during irradiation with the ion beam, the shifting mechanism having a temperature control mechanism for controlling temperature of at least one of the shield holding member and the sample stand; and a sample holding member disposed between the shield and the sample, the sample holding member deforming following deformation of the sample during irradiation with the ion beam, for example.Type: GrantFiled: April 28, 2014Date of Patent: January 31, 2017Assignee: Hitachi High-Technologies CorporationInventors: Asako Kaneko, Hisayuki Takasu, Hirobumi Mutou, Toru Iwaya, Mami Konomi
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Patent number: 9492797Abstract: Method of detection for droplet-based assays. In an exemplary method, an open end of a channel network may be placed into an emulsion. Droplets of the emulsion may be driven along a flow path from the open end, through a confluence region where a dilution fluid is introduced into the flow path from at least one dilution inlet channel to increase an average distance between droplets, and through an examination region disposed downstream of the confluence region. Light may be detected from the examination region as droplets pass through. The channel network may include a droplet inlet channel that meets the at least one dilution inlet channel at the confluence region. The droplet inlet channel may form a tapered region that is sized such that droplets leave the tapered region in single file.Type: GrantFiled: January 20, 2014Date of Patent: November 15, 2016Assignee: Bio-Rad Laboratories, Inc.Inventors: Anthony J. Makarewicz, Amy L. Hiddessen
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Patent number: 9464266Abstract: In order to enable the inside of a constant-temperature device to be sterilized using sterilization gas and prevent contamination during incubation, even when provided with an atmosphere measuring means having a CO2 sensor and an oxygen concentration sensor, a sensor unit (100) is arranged inside or close to an incubation chamber, and the internal atmosphere is measured by sucking-in the atmosphere of the incubation chamber (2) by means of an air-flow generation means (20). Furthermore, high-precision filters (23, 24) are arranged in the flow channel through which the atmosphere within the incubation chamber (2) is taken-in/discharged, preventing bacteria and/or cells from flowing in, and also preventing the diffusion of sterilization gas to the inside of a sensor unit (100) during sterilization.Type: GrantFiled: June 11, 2012Date of Patent: October 11, 2016Assignee: RORZE CORPORATIONInventors: Seishi Yamashita, Tomonori Miwada
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Patent number: 9115336Abstract: Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.Type: GrantFiled: May 25, 2011Date of Patent: August 25, 2015Assignees: Muffin Incorporated, Cook Biotech IncorporatedInventors: Michael C. Hiles, Chad E. Johnson, Neal E. Fearnot, Thomas Payne, Ronald Jankowski
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Patent number: 9040288Abstract: This patent application describes an integrated apparatus for processing polynucleotide-containing samples, and for providing a diagnostic result thereon. The apparatus is configured to receive a microfluidic cartridge that contains reagents and a network for processing a sample. Also described are methods of using the apparatus.Type: GrantFiled: March 26, 2007Date of Patent: May 26, 2015Assignee: HandyLab, Inc.Inventors: Kalyan Handique, Sundaresh N. Brahmasandra, Karthik Ganesan, Betty Wu, Nikhil Phadke, Gene Parunak, Jeff Williams
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Patent number: 9040292Abstract: Provided is constant-temperature equipment wherein maintenance is facilitated with the least failure, and highly reliable culturing and testing can be carried out. Mechanical and electrical structures are eliminated from the inside of a temperature-controlled chamber (15) by using a non-contact magnetic arrangement as a drive transmission for a sample table (5) and a sample table drive (6), thus reducing failure and enhancing maintainability. In addition, a conveyor (11) is provided with a pass box to minimize change in atmosphere during conveying. The sample table drive (6) and the conveyor (11) can be attached removably to the temperature-controlled chamber (15) to permit sterilization at high temperature.Type: GrantFiled: June 30, 2009Date of Patent: May 26, 2015Assignee: Rorze CorporationInventor: Seishi Yamashita
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Patent number: 9040291Abstract: Constant-temperature equipment wherein mechanical and electrical structures are eliminated from the inside of a temperature-controlled chamber (15) by using a non-contact magnetic arrangement as a drive transmission for a sample table (5) and a sample table drive mechanism (6), thus reducing failure and enhancing maintainability. In addition, a conveyance mechanism (11) is provided with a pass box adjacent which sliding shielding plates (9) are stacked vertically, and the shielding plates (9) are linked with the conveyance mechanism (11) by an engaging mechanism provided in the conveyance mechanism (11) to allow the plates to be opened and closed by a travel mechanism (12), thus simplifying the structure and minimizing change in atmosphere during conveying. The sample table drive mechanism (6) and the conveyance mechanism (11) can be attached removably to the temperature-controlled chamber (15) to permit sterilization at high temperature.Type: GrantFiled: June 30, 2009Date of Patent: May 26, 2015Assignee: Rorze CorporationInventor: Seishi Yamashita
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Publication number: 20150140570Abstract: An incubator including a container having an interior chamber capable of receiving a sample holder having samples, a lower heated surface disposed within the interior chamber of the container, and a movement mechanism coupled to the container and the lower heated surface. In one embodiment, the movement mechanism is configured to move the lower heated surface to a position in which the lower heated surface essentially contacts the sample holder. A method of rapidly heating and incubating a sample for biochemical or immunological testing is further disclosed.Type: ApplicationFiled: May 28, 2013Publication date: May 21, 2015Inventors: Haojun Fu, Daniel M. Mueth, Sergio O. Guevara, Neil Rosenbaum
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Patent number: 9034635Abstract: A thermocycler apparatus and method for rapidly performing the PCR process employs at least two thermoelectric modules which are in substantial spatial opposition with an interior space present between opposing modules. One or multiple sample vessels are placed in between the modules such that the vessels are subjected to temperature cycling by the modules. The sample vessels have a minimal internal dimension that is substantially perpendicular to the modules that facilitates rapid temperature cycling. In embodiments of the invention the sample vessels may be deformable between: a) a shape having a wide mouth to facilitate filling and removing of sample fluids from the vessel, and b) a shape which is thinner for conforming to the sample cavity or interior space between the thermoelectric modules of the thermocycler for more rapid heat transfer.Type: GrantFiled: February 19, 2009Date of Patent: May 19, 2015Assignee: Streck, Inc.Inventors: Joel R. Termaat, Hendrik J. Viljoen, Scott E. Whitney
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Publication number: 20150118745Abstract: A cell culture system having a cell culture vessel, a composition controlling fluid storage vessel, a culture fluid composition controlling means having an inlet and an outlet for a cell culture fluid, an inlet-connected fluid feeding circuit from the cell culture vessel to the inlet of the culture fluid composition controlling means, an outlet-connected fluid feeding circuit from the cell culture vessel to the outlet of the culture fluid composition controlling means, a means which perfuses the cell culture fluid from the inlet-connected fluid feeding circuit to the outlet-connected fluid feeding circuit through the culture fluid composition controlling means, and a means which controls the amount of fluid in the cell culture vessel, in which compositions of the cell culture fluid in the cell culture vessel and compositions of the composition controlling fluid in the composition controlling fluid storage vessel can be controlled in a continuous manner.Type: ApplicationFiled: April 24, 2013Publication date: April 30, 2015Applicants: ASAHI KASEI KABUSHIKI KAISHA, TOKYO WOMEN'S MEDICAL UNIVERSITYInventors: Ushio Iwamoto, Michi Sato, Kanako Konishi, Katsuhisa Matsuura, Tatsuya Shimizu, Teruo Okano
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Patent number: 9012211Abstract: A harvested sample preparation system includes an operation isolator 3 in which aseptic manipulation is executed, a harvested sample preparation personal box 4 which can be connected with the operation isolator 3 and a storage 5 that stores a plurality of harvested sample preparation personal boxes 4. The harvested sample preparation personal box 4 includes a first housing chamber 4Aa and a second housing chamber 4Ab, and a fluid appropriate for cell culturing is supplied to the first housing chamber 4Aa from a fluid supply pipe 16 included by the storage 5 while cooling fluid is supplied to the second housing chamber 4Ab from a cooling fluid supply pipe 18. The harvested sample preparation system that prevents mix-up and cross-contamination with a simple configuration can be provided.Type: GrantFiled: June 22, 2010Date of Patent: April 21, 2015Assignees: Cellseed Inc., Shibuya Kogyo Co., Ltd.Inventors: Manabu Mizutani, Yoshiko Nohmi, Kenji Yoneda