Method Of Culturing Cells In Suspension Patents (Class 435/383)
  • Patent number: 10233231
    Abstract: This invention provides an improved process for manufacturing a Rabies monoclonal antibody (HuMab 17C7) that results in low osmolality, minimum secondary metabolites like ammonia and lactate, enhanced cell growth and productivity, minimum aggregation or degradation of monoclonal antibody during purification, thereby improving potency of monoclonal antibody (HuMab 17C7) as compared to human rabies immunoglobulin (hRIG).
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: March 19, 2019
    Assignee: Serum Institute of India Private Limited
    Inventors: Dhere Rajeev Mhalasakant, Pisal Sambhaji Shankar, Peddi Reddy Srinivas Reddy, Singh Digamber Chahar, Pardeep Gupta
  • Patent number: 10144945
    Abstract: A layered, microfluidic living cell array is disclosed. The cell array comprises a first layer comprising at least one cell culture channel; a second layer comprising at least one microfluidic channel; and a third layer, disposed between the first layer and the second layer. The third layer comprises a filter membrane with a plurality of pores, each pore fluidly connecting the microfluidic channel to the cell culture channel.
    Type: Grant
    Filed: April 21, 2014
    Date of Patent: December 4, 2018
    Assignees: Research Foundation of the City University of New York, Memorial Sloan Kettering Cancer Center
    Inventors: Sihong Wang, Zeynep Dereli-Korkut, Xuejun Jiang
  • Patent number: 10030220
    Abstract: A single-use bioreactor, suitable in particular for use in a parallel bioreactor system for applications in cell culture and/or microbiology, a head plate for such a single-use bioreactor and a process for manufacturing the single-use bioreactor and the head plate. The single-use bioreactor comprises a head plate, a dimensionally stable container and a mixer, the head plate and the container enclosing a reaction chamber, and the mixer having a mixer shaft and a stirring member, said mixer shaft being mounted rotatably about a rotational axis in a bearing and the stirring member being fastened torsionally rigidly to the mixer shaft. The mixer and the bearing are arranged entirely within the reaction chamber and the mixer shaft has a magnetic portion which is arranged and adapted in such a way that it can be coupled magnetically in an axial direction to a rotary drive.
    Type: Grant
    Filed: June 13, 2013
    Date of Patent: July 24, 2018
    Assignee: EPPENDORF AG
    Inventors: Matthias Arnold, Heinz Gerhard Kohn, Nico Gulzow, Sven Eikelmann, Sebastian Selzer, Jochen Beese, Christopher Gunther, Eckart Kopowski
  • Patent number: 10030221
    Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: July 24, 2018
    Assignee: DANISCO US INC.
    Inventors: Jimmy Chan, Rachel E. Muir, William Throndset
  • Patent number: 9956251
    Abstract: The present invention relates to a preparation method for a therapeutic agent of a bead-type chondrocyte and, more specifically, to: a preparation method for a therapeutic agent of a bead-type chondrocyte without a support, comprising the steps of: a) dispensing chondrocytes and/or cells having chondrogenic differentiation capability in a 96-well deep well plate having a V-shaped bottom; b) centrifuging the plate; c) three-dimensionally culturing the plate in an incubator; and d) recovering pellets from each well, thereby being capable of easily and stably preparing cartilage tissues of a uniform quality, in large quantities; and a therapeutic agent of a bead-type chondrocyte without a support, prepared by the method, having effectiveness to repair damage by simply being implanted into cartilage damage by injection.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: May 1, 2018
    Assignee: BIO SOLUTION CO., LTD.
    Inventors: Jung Sun Lee, Jin Yeon Lee, Byung Chul Chae, Young Sook Son, Song Sun Chang
  • Patent number: 9952204
    Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.
    Type: Grant
    Filed: May 6, 2011
    Date of Patent: April 24, 2018
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: James Hickman, Xiufang Guo, Mercedes Gonzalez, Maria Stancescu
  • Patent number: 9902928
    Abstract: The present invention provides a production module for large-scale production of cells and/or cell-derived products such as antibodies or virus; a production suite comprising a plurality of functionally connected production modules of the invention; and a method for large-scale production of cells and/or cell-derived products using the production modules and/or production suites of the invention.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: February 27, 2018
    Assignee: BIOVEST INTERNATIONAL, INC.
    Inventors: Mark Hirschel, Robert J. Wojciechowski, Kim Arneson
  • Patent number: 9527401
    Abstract: A 12 volt automotive battery system includes a first battery coupled to an electrical system, in which the first battery includes a first battery chemistry. The first battery is charged with a relatively constant first voltage, in which an alternator outputs the relatively constant first voltage. The 12 volt automotive battery system further includes a second battery coupled in parallel with the first battery and selectively coupled to the electrical system via a DC/DC converter, in which the second battery includes a second battery chemistry that has a higher coulombic efficiency than the first battery chemistry. The DC/DC converter boosts the first voltage to a second voltage to charge the second battery during regenerative braking, in which the second voltage is higher than a maximum charging voltage of the first battery.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: December 27, 2016
    Assignee: Johnson Controls Technology Company
    Inventors: Daniel B. Le, Perry M. Wyatt, Ryan S. Mascarenhas, Brian C. Sisk
  • Patent number: 9469865
    Abstract: The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cells culture comprising the cells, the biological substance and the cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.
    Type: Grant
    Filed: February 7, 2014
    Date of Patent: October 18, 2016
    Assignee: DPX Holdings B.V.
    Inventors: Gerben Meile Zijlstra, Robert Patrick Hof, Jacob Schilder
  • Patent number: 9447375
    Abstract: Methods of treating lysis in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture comprising with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 10, 2015
    Date of Patent: September 20, 2016
    Assignee: HELIAE DEVELOPMENT LLC
    Inventors: Laura Carney, Kristine Sorensen
  • Patent number: 9441035
    Abstract: Cell culture media are provided herein as are methods of using the media for cell culture and antibody production from cells. Compositions comprising antibodies and fragments thereof, produced by the methods herein are also provided.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 13, 2016
    Assignee: GENENTECH, INC.
    Inventors: Veronica Carvalhal, Natarajan Vijayasankaran, Lauren Brown, Thomas DiRocco, Nathan McKnight
  • Patent number: 9365813
    Abstract: The invention relates to a bioreactor system characterized by its capacity in cultivating cells in all three states: static, dynamic, or alternating between static and dynamic states in the same cell culture container or containers, with the even distribution of cells in cell static culture following a dynamic culture. In the invented bioreactor system, the combined application of the magnetically controlled agitation and the cell culture container inversion as well as the combined application of the vertical rotating culture and horizontal static culture are the two strategies in building ideal bioreactors for the cell culture alternating between static and dynamic states in the same cell culture container, which can minimize the sheer-stress and provide cells an ideal metabolic environment.
    Type: Grant
    Filed: September 29, 2013
    Date of Patent: June 14, 2016
    Inventor: Yongxin Zhang
  • Patent number: 9200335
    Abstract: A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: December 1, 2015
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz K. Niazi
  • Patent number: 9133493
    Abstract: The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: September 15, 2015
    Assignee: AMGEN INC.
    Inventors: Matthew I. Jerums, Amanda Kano, Henry Lin, Shun Luo, Jian Wu, Rebecca E. McCoy, Arvia E. Morris
  • Patent number: 9068215
    Abstract: A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: June 30, 2015
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz K. Niazi
  • Patent number: 9060915
    Abstract: Improving cell therapy and tissue regeneration in a patient suffering from a cardiovascular or a neurological disease by treating a tissue of the patient with shock waves and/or applying to the patient a therapeutically effective amount of stem cells and/or progenitor cells. Such treatment increases expression of chemoattractants, pro-angiogenic factors, and pro-survival factors. The chemoattractants can be, for example, vascular endothelial growth factor (VEGF) or stromal cell derived factor 1 (SDF-1). For example, the treated tissue can be located in the patient's heart or in a skeletal muscle of the patient, and the shock waves can be extracorporeal shock waves (ESW) or intracorporeal shock waves. The cardiovascular disease can have an ischemic or non-ischemic etiology. For example, the cardiovascular disease can be a myocardial infarction, ischemic cardiomyopathy, or a dilatative cardiomyopathy. For example, the neurological disease can be a peripheral neuropathy or neuropathic pain.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: June 23, 2015
    Assignee: Dornier MedTech Systems, GmbH
    Inventors: Andreas Lutz, Harald Eizenhofer, Andreas Michael Zeiher, Stefanie Dimmeler, Christopher Heeschen, Alexandra Aicher
  • Patent number: 9045733
    Abstract: A method of increasing the rate of growth, useful product production, or protein expression of a microorganism includes the step of exposing the microorganism to ultrasound having a frequency greater than about 1 MHz.
    Type: Grant
    Filed: August 26, 2009
    Date of Patent: June 2, 2015
    Assignee: Intelligentnano Inc.
    Inventors: Jie Chen, James Xing, Woon T. Ang
  • Patent number: 9040290
    Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 26, 2015
    Assignee: Corning Incorporated
    Inventors: Gregory Roger Martin, Allison Jean Tanner
  • Publication number: 20150132845
    Abstract: Devices, systems, and methods for continuous cell culture and other reactions are generally described. In some embodiments, chambers (e.g., cell growth chambers) including at least a portion of a wall formed of a flexible member are provided. A retaining structure can be incorporated outside and proximate to the chamber such that when liquid is added to the chamber, the flexible member is consistently and predictably deformed, and a consistent volume of liquid is added. The flexible member can be formed of, in some embodiments, a gas-permeable medium. In some embodiments, reaction chambers can be arranged in a fluidic loop, and a bypass channel can be used to introduce and/or extract fluid from the loop without affecting loop operation.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 14, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Rajeev Jagga Ram, Kevin Shao-Kwan Lee
  • Publication number: 20150125428
    Abstract: A substantially enriched mammalian multipotent vascular stem cell (MVSC) population is provided, as well as compositions comprising the population. Methods are provided for the isolation, purification, and culture of the MVSCs. The MVSCs are useful in various applications, which are also provided.
    Type: Application
    Filed: January 29, 2013
    Publication date: May 7, 2015
    Applicant: The Regents of the University of California
    Inventors: Song Li, Zhenyu Tang, Aijun Wang
  • Patent number: 9023647
    Abstract: An improved monocyte activation test is described that is better able to detect non-endotoxin pyrogens in medical products, in which a sample is incubated with a monocyte-containing reagent in an assay system comprising at least one surface comprising polypropylene. The invention also concerns assay systems for use in these tests that include at least one microtiter well having at least one interior surface comprising polypropylene and having a shape such that monocyte-containing reagent is concentrated in the well to provide greater cell to cell contact. The invention also relates to a diagnostic kit that can be used to test for the presence of non-endotoxin pyrogens in a sample.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: May 5, 2015
    Assignees: Baxter International Inc., Baxter Healthcare SA, The Secretary of State for Health
    Inventors: Stephen Poole, Mehul Patel
  • Patent number: 9018007
    Abstract: The present invention relates to the use of cell cultures in the open continuous mode, to a method for selecting static cell variants or cell variants which proliferate in suspension, to a culture substrate and to a device suitable for implementing this method.
    Type: Grant
    Filed: February 20, 2009
    Date of Patent: April 28, 2015
    Assignee: Metabolium
    Inventors: Bernard Kudla, Pierre-Yves Chesneau, Yann Beaujouan
  • Patent number: 9005971
    Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber that is adapted to hold a fluid. A sparger is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparger. At least a portion of the sparger includes a sparging sheet formed from a flexible sheet of a gas permeable material. A tubular port or tube is coupled with the bag so that a passage bounded by the tubular port or tube communicates with the compartment.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: April 14, 2015
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones, Jeremy K. Larsen
  • Patent number: 9005967
    Abstract: The present invention provides a method for improving iPS cell generation efficiency, which comprises a step of introducing a Myc variant having the following features: (1) having an activity to improve iPS cell generation efficiency which is comparative to, or greater than that of c-Myc; and (2) having a transformation activity which is lower than that of c-Myc; or a nucleic acid encoding the variant, in a nuclear reprogramming step. Also, the present invention provides a method for preparing iPS cells, which comprises a step of introducing the above Myc variant or a nucleic acid encoding the variant and a combination of nuclear reprogramming factors into somatic cells. Moreover, the present invention provides iPS cells comprising the nucleic acid encoding the Myc variant which can be obtained by the above method, and a method for preparing somatic cells which comprises inducing differentiation of the iPS cells.
    Type: Grant
    Filed: January 21, 2011
    Date of Patent: April 14, 2015
    Assignee: Kyoto University
    Inventors: Shinya Yamanaka, Masato Nakagawa
  • Patent number: 9005970
    Abstract: [PROBLEMS] Relating to a bioassay method with the use of cultured cells and cell culture for a therapeutic purpose in the case of determining the efficacy of a drug or the like or examining the toxicity thereof, it is intended to provide a method of culturing cells under regulation in the extension direction and a cell culture plate. [MEANS FOR SOLVING PROBLEMS] A cell culture plate having a plural number of side walls (1) and a plural number of space structures (3) for providing cultured cells which are formed by the side walls (1), wherein the side walls (1) are provided with openings (2) so that the space structures (3) are linked together. By using this cell culture plate, a method of culturing cells under regulation in the extension direction can be provided.
    Type: Grant
    Filed: January 11, 2006
    Date of Patent: April 14, 2015
    Assignees: Kuraray Co., Ltd., Ryosuke Nishio
    Inventors: Ryosuke Nishio, Taiji Nishi, Motohiro Fukuda, Yasuzo Kirita, Seiichi Kanai, Takenori Kitani
  • Patent number: 8980631
    Abstract: A method and device for creating hanging drop cell aggregates. The method and device includes a plurality of pegs that allow for high throughput culture of aggregates. Also disclosed are means of transferring formed aggregates to various destinations, such as well plates, scaffolding, tissues, or wounds. Use of the device permits aggregates to be prepared or created in larger quantities than current methods, and allows for them to be transferred more efficiently.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: March 17, 2015
    Assignee: University of Virginia Patent Foundation
    Inventors: Blair Taylor Stocks, Shayn Peirce Cottler, Adam J. Katz
  • Patent number: 8980630
    Abstract: The present invention relates to stem cells obtained from the amnion and their methods of obtaining and culturing. The present invention further relates to compositions comprising amnion-derived stems cells (ADSCs) and to methods of using ADSCs.
    Type: Grant
    Filed: June 28, 2007
    Date of Patent: March 17, 2015
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Dale Woodbury, Akiva J. Marcus
  • Publication number: 20150072420
    Abstract: As described below, the present invention features compositions and methods related to the isolation, culture and therapeutic use of CD31-expressing cells.
    Type: Application
    Filed: April 18, 2014
    Publication date: March 12, 2015
    Applicant: EMORY UNIVERSITY
    Inventors: Young-sup Yoon, Hyun-Jai Cho
  • Publication number: 20150056701
    Abstract: The present invention relates to a cell culture medium additive comprising a xanthan polysaccharide and a mannan polysaccharide, such as a glucomannan or a galactomannan, that supports the growth of cells in suspension, and to a cell culture medium containing the additive. More particularly, but not exclusively, the present invention relates to a cell culture medium additive that supports the formation of multicellular bodies such as spheroids, and prevents sedimentation of cells without adversely affecting the physical properties of the cell culture medium.
    Type: Application
    Filed: April 2, 2013
    Publication date: February 26, 2015
    Inventor: Anthony Davies
  • Publication number: 20150056702
    Abstract: A method is provided, including adding a phthalide to a stem cell medium to provide a phthalide-containing medium, and then culturing a stem cell using the phthalide-containing medium. The use of a phthalide in a medium for culturing stem cells optionally maintains the pluripotency of stem cells. The phthalide also enhances the generation efficiency of induced pluripotent stem cells to decrease the culture cost.
    Type: Application
    Filed: October 31, 2014
    Publication date: February 26, 2015
    Inventors: SHIH-PING LIU, SHINN-ZONG LIN, HORNG-JYH HARN, YING-JIUN CHIEN, CHIEN-YU HSU, CHENG-HSUAN CHANG
  • Patent number: 8962323
    Abstract: The invention provides a method of isolating dermal stem cells, having the steps of subjecting cells separated from the skin by enzyme treatment to suspension culture, and isolating cells positive for stem cell markers from the cultured cells.
    Type: Grant
    Filed: September 15, 2010
    Date of Patent: February 24, 2015
    Assignee: Shiseido Company, Ltd.
    Inventors: Tsutomu Soma, Haruyo Yamanishi
  • Patent number: 8961962
    Abstract: The present invention relates to methods of promoting the survival of cells by treating the cells with acid ceramidase. A kit for promoting ex vivo cell survival is also disclosed, as is a method of predicting in vitro fertilization outcome of a female subject.
    Type: Grant
    Filed: January 7, 2008
    Date of Patent: February 24, 2015
    Assignee: Mount Sinai School of Medicine
    Inventors: Edward H. Schuchman, Efrat Eliyahu, Nataly Shtraizent, Xingxuan He
  • Patent number: 8951793
    Abstract: Disclosed are methods of isolating and using a population of FOXP3+ regulatory T cells in a variety of preventative and therapeutic approaches to autoimmune diseases, graft-versus-host disease and transplant rejection.
    Type: Grant
    Filed: August 21, 2009
    Date of Patent: February 10, 2015
    Assignee: The United States of America, as represented by The Secretary, Department of Health and Human Services
    Inventors: Dat Tran, Ethan M. Shevach
  • Patent number: 8945867
    Abstract: The present invention relates to a process for producing a desired polypeptide using rat cells. Specifically, the present invention relates to a process for producing the polypeptide which comprises culturing rat cells such as YB2/3HL.P2.G11.16Ag.20 (hereinafter referred to as YB2/0), preferably rat cells to which a recombinant DNA comprising DNA encoding a desired polypeptide such as an immunologically functional molecule is introduced, in a medium which does not contain serum (hereinafter referred to as a serum-free medium). Among the desired polypeptides obtained by the process of the present invention, an antibody obtained by using a transformant of YB2/0 has a high antibody-dependent cell-mediated cytotoxic activity (hereinafter sometimes referred to as ADCC activity) and is useful as a pharmaceutical agent.
    Type: Grant
    Filed: February 2, 2009
    Date of Patent: February 3, 2015
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Tatsuya Ogawa, Yoshinobu Konno, Naohisa Akashi, Hiroshi Takasugi, Seiji Sugimoto, Keiichi Yano
  • Patent number: 8945894
    Abstract: A method for directing, enhancing, and accelerating mesenchymal stem cell functions using alternating electric current. Mesenchymal stem cells are preferentially directed to either osteoblast or chondrocyte lineages, but not to the adipocyte lineage. when exposed to alternating electric current.
    Type: Grant
    Filed: September 28, 2012
    Date of Patent: February 3, 2015
    Inventors: Courtney M. Creecy, Rena Bizios
  • Patent number: 8945920
    Abstract: The invention concerns a method for culturing cells derived from the adipose tissue and in particular the stromal vascular fraction (SVF) to induce formation of cardiomyocytes, the use of the cells obtained by said culture method to reconstitute an ischemized cardiac zone, in particular following an infarction, as well as a pharmaceutical composition containing said cells. The method for obtaining cardiac cells comprises at least the following steps: a) selecting cardiomyogenic cells from the stromal vascular fraction (SVF); b) culturing the cells selected at step a) in a liquid medium optimized for expanding ex vivo the cardiomyogenic cells; c) maintaining and expanding said cells by successive passes in the liquid medium; and d) obtaining cardiac cells.
    Type: Grant
    Filed: January 26, 2007
    Date of Patent: February 3, 2015
    Assignee: Centre National de la Recherche Scientifique
    Inventors: Louis Casteilla, Valérie Planat-Benard, Luc Penicaud, Carine Chanut
  • Patent number: 8940535
    Abstract: Methods and compositions are provided for isolation of proliferating cells. In particular, the methods enrich stem cells in a mixture of stem cells and non-stem cells, and in some cases the non-stem cells may be differentiated cells. The methods exploit the non-adherent property of stem cells, as opposed to the adherent property of differentiating cells, by serially passaging the suspended cells in liquid media.
    Type: Grant
    Filed: November 4, 2004
    Date of Patent: January 27, 2015
    Assignees: University of Maryland, Baltimore, The United States of America as Represented by the Dept of Veterans Affairs
    Inventors: G. David Trisler, Christopher T. Bever, Jr., James E. Goolsby, Bernard M. Pessac
  • Patent number: 8940537
    Abstract: The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal™ medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters.
    Type: Grant
    Filed: January 25, 2012
    Date of Patent: January 27, 2015
    Assignee: Hadasit Medical Research Services & Development Limited
    Inventors: Benjamin Reubinoff, Debora Steiner
  • Patent number: 8936939
    Abstract: The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate. The invention also provides methods to transform normal primary cells so cultured into “cancer stem cells.” The genetically defined cancer stem cell tumor model mimics the behavior of the disease closely, e.g., the cells are invasive, hormone responsive and metastatic when injected into mice. The tumor cells express genes that are specific to cancer stem cells identified in patient samples.
    Type: Grant
    Filed: July 23, 2012
    Date of Patent: January 20, 2015
    Assignees: Whitehead Institute for Biomedical Research, The Brigham and Women's Hospital, Inc.
    Inventors: Tan A. Ince, Robert A. Weinberg
  • Patent number: 8927281
    Abstract: The present invention relates to compounds and compositions for expanding the number of CD34+ cells for transplantation. The invention further relates to a cell population comprising expanded hematopoietic stem cells (HSCs) and its use in autologous or allogeneic transplantation for the treatment of patients with inherited immunodeficient and autoimmune diseases and diverse hematopoietic disorders to reconstitute the hematopoietic cell lineages and immune system defense.
    Type: Grant
    Filed: October 29, 2009
    Date of Patent: January 6, 2015
    Assignees: IRM LLC, The Scripps Research Institute
    Inventors: Anthony E. Boitano, Michael Cooke, Shifeng Pan, Peter G. Schultz, John Tellew, Yongqin Wan, Xing Wang
  • Patent number: 8921093
    Abstract: The invention relates to an arrangement (a measurement device) for on-line measurements on cells, in particular for measuring soluble analytes and dissolved gases on samples in a sample area.
    Type: Grant
    Filed: July 23, 2007
    Date of Patent: December 30, 2014
    Assignee: BioCer Entwicklungs GmbH
    Inventors: Daniel Seitz, Helmar Mayr, Guenter Ziegler, Winfried Vonau, Frank Gerlach, Sigrun Herrmann
  • Patent number: 8906685
    Abstract: The present disclosure relates general to devices, systems, and methods of using such devices in creating and handling hanging drops of fluid. The present disclosure also relates to cell culture devices, methods and/or systems of using such devices as well as the use of cell culture devices, for example, for research and high throughput screening.
    Type: Grant
    Filed: January 28, 2011
    Date of Patent: December 9, 2014
    Assignees: The Regents of The University of Michigan, 3D Biomatrix, Inc.
    Inventors: Shuichi Takayama, Yi-Chung Tung, Amy Yu-Ching Hsiao, Edward Jan
  • Publication number: 20140356336
    Abstract: The object of the invention is to provide a technique of suppressing tumorigenesis in IPS cells and inducing differentiation into target differentiated cells. In use of a statin and a differentiation inducer, iPS cells are differentiated into target differentiated cells, whereby iPS cells can be differentiated into differentiated cells in which tumorigenesis is suppressed.
    Type: Application
    Filed: December 27, 2012
    Publication date: December 4, 2014
    Inventor: Hiroshi Egusa
  • Patent number: 8895300
    Abstract: The present invention relates to methods for production of undifferentiated or differentiated embryonic stem cell aggregate suspension cultures from undifferentiated or differentiated embryonic stem cell single cell suspensions and methods of differentiation thereof.
    Type: Grant
    Filed: November 8, 2012
    Date of Patent: November 25, 2014
    Assignee: Viacyte, Inc.
    Inventor: Thomas C Schulz
  • Patent number: 8883502
    Abstract: The invention provides methods for obtaining neural stem cells from a mammalian embryonic or inducible pluripotent stem cell population comprising culturing mammalian embryonic or inducible pluripotent stem cells in a cell culture medium having a leukemia inhibitory factor (LIF), an inhibitor of glycogen synthase kinase 3 (GSK3), and an inhibitor of transforming growth factor ? (TGF-?) under suitable conditions and obtaining isolated neural stem cells therefrom.
    Type: Grant
    Filed: September 9, 2011
    Date of Patent: November 11, 2014
    Assignee: The Regents of the University of California
    Inventors: Kang Zhang, Sheng Ding
  • Patent number: 8883504
    Abstract: The object of the present invention is to provide a differentiation inhibiting agent which allows culture of a stem cell or an embryonic stem cell in an undifferentiated state without use of any feeder cell, a method for culturing using the same, a cell culture liquid using the same, and a cell prepared by culturing using this differentiation inhibiting agent. The present invention provides a differentiation inhibiting agent which comprises a low molecular weight compound, especially a tetrahydroisoquinoline derivative, as an active ingredient; a method for safely culturing a stem cell in large scale in undifferentiated state in the absence of feeder cell which comprises culturing a stem cell by using a tetrahydroisoquinoline derivative; a culture liquid for stem cells comprising a tetrahydroisoquinoline derivative; and a cell which is obtained by culture using a tetrahydroisoquinoline derivative as a differentiation inhibiting agent.
    Type: Grant
    Filed: September 18, 2008
    Date of Patent: November 11, 2014
    Assignee: Asahi Kasei Kabushiki Kaisha
    Inventors: Tomoyuki Miyabayashi, Masashi Yamamoto
  • Publication number: 20140314675
    Abstract: An objective of the present invention is to provide: a cancer stem cell isolated using a cell marker; a substantively homogeneous cancer stem cell population including said cancer stem cell; and a method of preparing said cancer stem cell population. Another objective of the present invention is to provide: a method for separating cancer stem cells with a high proliferative potential and those with a low proliferative potential; a method for inducing cancer stem cells to have a different proliferative potential; and cancer stem cells separated or induced by these separation or induction methods. A further objective of the present invention is to provide: a method of screening for pharmaceuticals using said cancer stem cell or cancer stem cell population; a method for detecting the presence of said cancer stem cell or cancer stem cell population and for identifying or quantifying the same.
    Type: Application
    Filed: September 7, 2012
    Publication date: October 23, 2014
    Inventors: Tatsumi Yamazaki, Hisafumi Okabe, Shinta Kobayashi, Takeshi Watanabe, Koichi Matsubara, Atsuhiko Kato, Masami Suzuki
  • Publication number: 20140308695
    Abstract: The present invention concerns bioactive renal cell populations, in particular a B2 cell population comprising an enriched population of tubular cells and wherein the renal cell population is depleted of a B1 cell population, renal cell constructs, and methods of screening test agents using the bioactive renal cell populations.
    Type: Application
    Filed: November 5, 2012
    Publication date: October 16, 2014
    Inventors: Andrew T. Bruce, Russell W. Kelley, Timothy A. Bertram, Sumana Choudhury
  • Patent number: 8859280
    Abstract: A composition and method for in vitro fertilization is provided which uses culture media comprising elevated concentrations of lipoic acid. More specifically, the invention provides culture media for developmental cells having a lipoic acid concentration of 5 ?M to 40 ?M. Culture media that include lipoic acid at concentrations within the identified range are able to provide blastocysts with increased survival, increased cell numbers, increased inner cell masses and/or increased percentage of the total mass made up by the inner cell compared to blastocysts cultured in a control medium.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: October 14, 2014
    Assignee: Vitrolife Sweden AB
    Inventors: David K Gardner, Mark G Larman, Donald Linck
  • Publication number: 20140295545
    Abstract: A pre-programmed non-feedback continuous feeding method based on mass balance of the substrate in the bioreactor for use in culture growth and maintenance is provided. The disclosed method does not rely on instrument, probe or operator feedback. The method provides an efficient and effective alternative to bolus feeding.
    Type: Application
    Filed: September 14, 2012
    Publication date: October 2, 2014
    Applicant: AMGEN INC.
    Inventors: Henry Lin, Jeremy Bezaire