Method Of Culturing Cells In Suspension Patents (Class 435/383)
  • Patent number: 11773364
    Abstract: An automated cell culturing device, which expands, detaches and prepares cells, ready to be implanted in vivo is disclosed. The device is composed by a multi-layered cell culture chamber, a cell preparation chamber, and critical parameters control units which automatically drive the cell culture medium circulation, change and refill. The device according to the invention is characterized in that all the components contacting cells and culture medium constitute a totally disposable “cartridge” in order to avoid cross-contamination and improve safety. The device is particularly useful for expanding and preparing mesenchymal stem cells for osteoarthritis (OA) therapy, and for other cell based therapies in mammals.
    Type: Grant
    Filed: April 8, 2015
    Date of Patent: October 3, 2023
    Assignee: VivaBioCell S.p.A.
    Inventors: Giacomo Cattaruzzi, Massimo Moretti, Antonio Sfiligoj, Francesco Curcio
  • Patent number: 11597903
    Abstract: Methods of protein production in continuous perfusion mammalian cell culture bioreactors are provided. Methods for continuous perfusion culture by allowing cells to self-regulate the rate of addition of perfusion medium to the bioreactor via a pH change are presented. Compositions comprising the perfusion medium as well as the process advantages of using hi-end pH control of perfusion or HIPCOP are also presented.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: March 7, 2023
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Gregory Walter Hiller, Matthew Paul Gagnon, Ana Maria Ovalle
  • Patent number: 11590470
    Abstract: A liquid process assembly, the assembly including a length of pipework, and a reversible pump for selectively reciprocally moving liquid through the pipework.
    Type: Grant
    Filed: July 12, 2019
    Date of Patent: February 28, 2023
    Assignee: NiTech Solutions Limited
    Inventor: Alastair William Barton
  • Patent number: 11505785
    Abstract: Provided is a cell culture method comprising the step of culturing cells using a medium containing a laminin fragment having integrin binding activity, the method not comprising the step of coating a culture vessel with a laminin or a laminin fragment before seeding the cells in the culture vessel. The cell culture method of the present invention uses a smaller amount of a laminin fragment and still achieves a comparable culture efficiency as compared with the conventional cell culture method that uses a culture vessel precoated with a laminin or a laminin fragment.
    Type: Grant
    Filed: November 8, 2016
    Date of Patent: November 22, 2022
    Assignee: Kyoto University
    Inventors: Hirofumi Suemori, Takamichi Miyazaki
  • Patent number: 11427808
    Abstract: To provide a method for serum-free culture of human cartilage cells and a serum-free culture medium. A method for serum-free culture of cartilage cells, said method comprising: an enzymatic treatment step for treating a human cartilage cell-containing tissue with a protease; an inhibitor-treatment step for, after the enzymatic treatment step, treating the tissue with an inhibitor for the aforesaid protease; and a culture step for, after the inhibitor-treatment step, culturing the tissue in a serum-free culture medium that contains kartogenin and/or SAG, ITS, FGF2 and hydrocortisone. A serum-free culture medium for culturing cartilage cells, said serum-free culture medium containing kartogenin and/or SAG, ITS, FGF2 and hydrocortisone.
    Type: Grant
    Filed: October 26, 2015
    Date of Patent: August 30, 2022
    Assignee: Regenesis Science Co., Ltd.
    Inventors: Hiroko Yanaga, Katsu Yanaga
  • Patent number: 11390663
    Abstract: Improved methods for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: July 19, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Shawn Lawrence, Michelle LaFond, Ann Kim, Amy S. Johnson, Brandon Veres, Mark Czeterko, Kristen Whalen
  • Patent number: 11371007
    Abstract: A bioprocessing system includes a first fluid assembly having a first fluid assembly line connected to a first port of a first bioreactor vessel though a first bioreactor line of a first bioreactor vessel, a second fluid assembly having a second fluid assembly line connected to a second port of the first bioreactor vessel through a second bioreactor line of the first bioreactor vessel, and an interconnect line providing for fluid communication between the first fluid assembly and the second fluid assembly, and for fluid communication between the second bioreactor line of the first bioreactor vessel and the first bioreactor line of the first bioreactor vessel.
    Type: Grant
    Filed: February 8, 2019
    Date of Patent: June 28, 2022
    Inventors: Weston Blaine Griffin, Alex D Corwin, Xiaohua Zhang, Reginald Donovan Smith, Zhen Liu, Chengkun Zhang, Vandana Keskar, Brian Michael Davis, Kashan Shaikh
  • Patent number: 11291690
    Abstract: The present invention provides muscle-derived progenitor cells that show long-term survival following transplantation into body tissues and which can augment soft tissue following introduction (e.g. via injection, transplantation, or implantation) into a site of soft tissue. Also provided are methods of isolating muscle-derived progenitor cells, and methods of genetically modifying the cells for gene transfer therapy. The invention further provides methods of using compositions comprising muscle-derived progenitor cells for the augmentation and bulking of mammalian, including human, soft tissues in the treatment of various cosmetic or functional conditions, including malformation, injury, weakness, disease, or dysfunction. In particular, the present invention provides treatments and amelioration of symptoms for gastro-esophageal pathologies like gastro-esophageal reflux.
    Type: Grant
    Filed: February 10, 2020
    Date of Patent: April 5, 2022
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: Michael B. Chancellor, Jay Pasricha, Ronald Jankowski, Ryan Pruchnic
  • Patent number: 11286460
    Abstract: Cell culture media are provided herein as are methods of using the media for cell culture and protein production from cells.
    Type: Grant
    Filed: March 24, 2021
    Date of Patent: March 29, 2022
    Assignee: Regeneron Pharmaceuticals, Inc.
    Inventors: Wei Xue, John Chen, Carolyn Scott, Theodore Loney, Nathaniel Golden
  • Patent number: 11248210
    Abstract: The present disclosure relates to a subfractionation culturing method of a stem cell and proliferation method of a monoclonal stem cell obtained using the same. According to the subfractionation culturing method of stem cells and the proliferation thereof of the exemplary embodiments of the present disclosure, it is advantage that monoclonal stem cells may be quickly obtained without contamination, and desired monoclonal stem cells may be largely obtained in a short time through the rapid proliferation, thereby being used for the preparation of stem cell-therapeutic agents.
    Type: Grant
    Filed: July 3, 2018
    Date of Patent: February 15, 2022
    Inventors: Sun Uk Song, Si Na Kim, Yun Kyoung Cho, Dong Sik Ham
  • Patent number: 11142734
    Abstract: The present invention relates to a novel microfluidic device and a microfluidic system for culturing cell samples based on: one or more culture wells (2) forming cell housing volumes for three-dimensional cell culture; one or more capillary channels (1) for circulating cells and/or fluid media, said channels (1) being connected to the wells (2) as well perfusion means; and an inlet port (3) and an outlet port (3?) for seeding cells and/or for circulating fluid in said channel (1), said ports (3, 3?) being connected to the ends of each capillary channel (1). The novel configuration of the ports (3, 3?) and their corresponding channels (1) allows known microfluidic perfusion media to be improved, dispensing with the use of complex elements for injecting the fluid media, such as syringe pumps, for example. The invention also allows two-dimensional and three-dimensional culture studies to be performed simultaneously.
    Type: Grant
    Filed: November 4, 2016
    Date of Patent: October 12, 2021
    Inventors: Luis J. Fernández Ledesma, Rosa Monge Prieto, Guillermo Llamazares Prieto, José María Ayuso Domínguez, María Virumbrales Muñoz, Jorge Santolaria Mazo, Ignacio Ochoa Garrido
  • Patent number: 10801005
    Abstract: A system for controlling a motion of a cell culture includes a tray adapted to hold a cell culture, a camera adapted to capture an image of the cell culture, and a device adapted to control a movement of the tray. The system also includes a processor adapted to determine a first movement of the tray, receive from the camera data representing an image of the cell culture, determine a characteristic of the cell culture based on the image data, determine a second movement of the tray based on the characteristic, the second movement being different from the first movement, and cause the tray to move in accordance with the second movement.
    Type: Grant
    Filed: September 13, 2017
    Date of Patent: October 13, 2020
    Assignee: VBC HOLDINGS LLC
    Inventors: Francesco Armani, Giacomo Cattaruzzi, Francesco Curcio, Massimo Moretti, Antonio Sfiligoj, Piero Fissore
  • Patent number: 10731126
    Abstract: The present invention relates to cell culture media compositions comprising deep eutectic solvents and/or ionic liquids.
    Type: Grant
    Filed: May 2, 2016
    Date of Patent: August 4, 2020
    Assignee: Merck Patent GmbH
    Inventors: Jochen Bastian Sieck, Michael Howard Rayner, Joerg Von Hagen, Claudia Knack
  • Patent number: 10676707
    Abstract: A culture system comprises: a preparatory culture vessel and a main culture vessel that accommodate cells and a solution; a main stage that holds the preparatory culture vessel and the main culture vessel; a connecting tube that connects the culture vessels; a valve that opens and closes the connecting tube; and a rotating mechanism that rotates the main stage and imparts a height difference between the culture vessels to transfer the cells and solution by dropping between the culture vessels.
    Type: Grant
    Filed: September 2, 2014
    Date of Patent: June 9, 2020
    Assignee: Universal Bio Research Co., Ltd.
    Inventor: Hideji Tajima
  • Patent number: 10577613
    Abstract: The present disclosure relates to engineered cells that include genetic alterations leading to up- or down-regulation of certain genes in the cells for improved production of a recombinant protein. Also provided are methods of preparing and using such cells.
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: March 3, 2020
    Assignee: Celltheon Corporation
    Inventors: Amita Goel, Nikhil Goel
  • Patent number: 10513689
    Abstract: A culture medium for human adipose tissue derived mesenchymal stem cells includes a keratinocyte-SFM basal medium, L-Cysteine, FGF and SDF-1 is disclosed. The culture medium is effective for growing mesenchymal stem cells at a high proliferation rate while maintaining the purity, characterization and undifferentiated state of the cells.
    Type: Grant
    Filed: April 29, 2016
    Date of Patent: December 24, 2019
    Inventor: Hyeonggeun Park
  • Patent number: 10494604
    Abstract: Invention embodiments described herein include methods and devices for stimulating mesenchymal stem cells in a stem cell source to differentiate into osteoblasts capable of forming bone. Devices and methods described include exposing a stem cell source, such as bone marrow aspirate, adipose tissue and/or purified allogenic stem cells, to an active agent, in a manner effective to form activated stem cells.
    Type: Grant
    Filed: April 5, 2016
    Date of Patent: December 3, 2019
    Assignee: DePuy Synthes Products, Inc.
    Inventors: Meredith Hans-Moore, Doug Buechter, Elliott Gruskin, Stephen Hornsby, Melissa Brown
  • Patent number: 10450540
    Abstract: The invention discloses a flexible bag assembly for cultivation of cells, comprising one or more bags forming a plurality of cultivation compartments, wherein a drain port in at least a first cultivation compartment is adapted to be fluidically connected with a second cultivation compartment upon opening of a valve means. It also discloses a bioreactor with the bag assembly mounted on a rocking tray and a method of cultivating cells in the assembly.
    Type: Grant
    Filed: April 27, 2015
    Date of Patent: October 22, 2019
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Klaus Gebauer, Andreas Castan, Camilla Estmer-Nilsson, Thomas Falkman, Anders Wilen, Michael Miller, Ralph Stankowski
  • Patent number: 10415018
    Abstract: This invention relates to a method for screening for a growth-promoting factor for pluripotent stem cells with a conditioned medium which is generated by culturing feeder cells in a serum-free medium that contains L-ascorbic acid, insulin, transferrin, selenium, and sodium bicarbonate and does not contain a serum nor serum replacement; a method for growing pluripotent stem cells via feeder-free culture using the conditioned medium; and a method for growing pluripotent stem cells by carrying out feeder-free culture of pluripotent stem cells cultured in advance on feeder cells in the serum-free medium.
    Type: Grant
    Filed: August 2, 2018
    Date of Patent: September 17, 2019
    Inventors: Tomohisa Kato, Yonehiro Kanemura, Tomoko Shofuda, Hayato Fukusumi
  • Patent number: 10258734
    Abstract: A method of modifying cells includes removing fluid including cells from a patient, contacting the removed fluid from the patient with at least one surface upon which at least one agent to interact at least one cell receptor is immobilized to modify cells in the fluid, and returning the fluid to the patient. The agent can, for example, be immobilized via covalent bonding or ionic bonding to the at least one surface. The fluid can, for example, be blood or a blood fraction. The agent can, for example, be an agonist, an antagonist or an inverse agonist.
    Type: Grant
    Filed: March 8, 2017
    Date of Patent: April 16, 2019
    Assignee: University of Pittsburgh—Of the Commonwealth System of Higher Education
    Inventors: William J. Federspiel, John Alston Kellum, Kai Singbartl
  • Patent number: 10233231
    Abstract: This invention provides an improved process for manufacturing a Rabies monoclonal antibody (HuMab 17C7) that results in low osmolality, minimum secondary metabolites like ammonia and lactate, enhanced cell growth and productivity, minimum aggregation or degradation of monoclonal antibody during purification, thereby improving potency of monoclonal antibody (HuMab 17C7) as compared to human rabies immunoglobulin (hRIG).
    Type: Grant
    Filed: July 6, 2016
    Date of Patent: March 19, 2019
    Assignee: Serum Institute of India Private Limited
    Inventors: Dhere Rajeev Mhalasakant, Pisal Sambhaji Shankar, Peddi Reddy Srinivas Reddy, Singh Digamber Chahar, Pardeep Gupta
  • Patent number: 10144945
    Abstract: A layered, microfluidic living cell array is disclosed. The cell array comprises a first layer comprising at least one cell culture channel; a second layer comprising at least one microfluidic channel; and a third layer, disposed between the first layer and the second layer. The third layer comprises a filter membrane with a plurality of pores, each pore fluidly connecting the microfluidic channel to the cell culture channel.
    Type: Grant
    Filed: April 21, 2014
    Date of Patent: December 4, 2018
    Assignees: Research Foundation of the City University of New York, Memorial Sloan Kettering Cancer Center
    Inventors: Sihong Wang, Zeynep Dereli-Korkut, Xuejun Jiang
  • Patent number: 10030221
    Abstract: The invention provides a culture plate made from a polymer incorporating a culture component releasable into culture media in the well, methods of culturing a microorganisms in the culture plate, and a methods of making the culture plate.
    Type: Grant
    Filed: September 20, 2013
    Date of Patent: July 24, 2018
    Assignee: DANISCO US INC.
    Inventors: Jimmy Chan, Rachel E. Muir, William Throndset
  • Patent number: 10030220
    Abstract: A single-use bioreactor, suitable in particular for use in a parallel bioreactor system for applications in cell culture and/or microbiology, a head plate for such a single-use bioreactor and a process for manufacturing the single-use bioreactor and the head plate. The single-use bioreactor comprises a head plate, a dimensionally stable container and a mixer, the head plate and the container enclosing a reaction chamber, and the mixer having a mixer shaft and a stirring member, said mixer shaft being mounted rotatably about a rotational axis in a bearing and the stirring member being fastened torsionally rigidly to the mixer shaft. The mixer and the bearing are arranged entirely within the reaction chamber and the mixer shaft has a magnetic portion which is arranged and adapted in such a way that it can be coupled magnetically in an axial direction to a rotary drive.
    Type: Grant
    Filed: June 13, 2013
    Date of Patent: July 24, 2018
    Assignee: EPPENDORF AG
    Inventors: Matthias Arnold, Heinz Gerhard Kohn, Nico Gulzow, Sven Eikelmann, Sebastian Selzer, Jochen Beese, Christopher Gunther, Eckart Kopowski
  • Patent number: 9956251
    Abstract: The present invention relates to a preparation method for a therapeutic agent of a bead-type chondrocyte and, more specifically, to: a preparation method for a therapeutic agent of a bead-type chondrocyte without a support, comprising the steps of: a) dispensing chondrocytes and/or cells having chondrogenic differentiation capability in a 96-well deep well plate having a V-shaped bottom; b) centrifuging the plate; c) three-dimensionally culturing the plate in an incubator; and d) recovering pellets from each well, thereby being capable of easily and stably preparing cartilage tissues of a uniform quality, in large quantities; and a therapeutic agent of a bead-type chondrocyte without a support, prepared by the method, having effectiveness to repair damage by simply being implanted into cartilage damage by injection.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: May 1, 2018
    Assignee: BIO SOLUTION CO., LTD.
    Inventors: Jung Sun Lee, Jin Yeon Lee, Byung Chul Chae, Young Sook Son, Song Sun Chang
  • Patent number: 9952204
    Abstract: A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions.
    Type: Grant
    Filed: May 6, 2011
    Date of Patent: April 24, 2018
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: James Hickman, Xiufang Guo, Mercedes Gonzalez, Maria Stancescu
  • Patent number: 9902928
    Abstract: The present invention provides a production module for large-scale production of cells and/or cell-derived products such as antibodies or virus; a production suite comprising a plurality of functionally connected production modules of the invention; and a method for large-scale production of cells and/or cell-derived products using the production modules and/or production suites of the invention.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: February 27, 2018
    Inventors: Mark Hirschel, Robert J. Wojciechowski, Kim Arneson
  • Patent number: 9527401
    Abstract: A 12 volt automotive battery system includes a first battery coupled to an electrical system, in which the first battery includes a first battery chemistry. The first battery is charged with a relatively constant first voltage, in which an alternator outputs the relatively constant first voltage. The 12 volt automotive battery system further includes a second battery coupled in parallel with the first battery and selectively coupled to the electrical system via a DC/DC converter, in which the second battery includes a second battery chemistry that has a higher coulombic efficiency than the first battery chemistry. The DC/DC converter boosts the first voltage to a second voltage to charge the second battery during regenerative braking, in which the second voltage is higher than a maximum charging voltage of the first battery.
    Type: Grant
    Filed: January 23, 2014
    Date of Patent: December 27, 2016
    Assignee: Johnson Controls Technology Company
    Inventors: Daniel B. Le, Perry M. Wyatt, Ryan S. Mascarenhas, Brian C. Sisk
  • Patent number: 9469865
    Abstract: The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cells culture comprising the cells, the biological substance and the cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture.
    Type: Grant
    Filed: February 7, 2014
    Date of Patent: October 18, 2016
    Assignee: DPX Holdings B.V.
    Inventors: Gerben Meile Zijlstra, Robert Patrick Hof, Jacob Schilder
  • Patent number: 9447375
    Abstract: Methods of treating lysis in cultures of Haematococcus pluvialis with hydrogen peroxide are described herein. The method comprises dosing the culture comprising with a concentration of hydrogen peroxide based on the stage of the cells in the culturing process and at a frequency to increase the likelihood of the cells surviving until the process of accumulating carotenoids, such as astaxanthin, is complete.
    Type: Grant
    Filed: July 10, 2015
    Date of Patent: September 20, 2016
    Inventors: Laura Carney, Kristine Sorensen
  • Patent number: 9441035
    Abstract: Cell culture media are provided herein as are methods of using the media for cell culture and antibody production from cells. Compositions comprising antibodies and fragments thereof, produced by the methods herein are also provided.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: September 13, 2016
    Assignee: GENENTECH, INC.
    Inventors: Veronica Carvalhal, Natarajan Vijayasankaran, Lauren Brown, Thomas DiRocco, Nathan McKnight
  • Patent number: 9365813
    Abstract: The invention relates to a bioreactor system characterized by its capacity in cultivating cells in all three states: static, dynamic, or alternating between static and dynamic states in the same cell culture container or containers, with the even distribution of cells in cell static culture following a dynamic culture. In the invented bioreactor system, the combined application of the magnetically controlled agitation and the cell culture container inversion as well as the combined application of the vertical rotating culture and horizontal static culture are the two strategies in building ideal bioreactors for the cell culture alternating between static and dynamic states in the same cell culture container, which can minimize the sheer-stress and provide cells an ideal metabolic environment.
    Type: Grant
    Filed: September 29, 2013
    Date of Patent: June 14, 2016
    Inventor: Yongxin Zhang
  • Patent number: 9200335
    Abstract: A bioreactor that combines the steps of recombinant expression and separation of a biological product by binding the secreted biological product with a resin, discarding the nutrient medium and eluting the biological product as a concentrated solution, eliminating the steps of sterile filtration and volume reduction. The method also allows loading of resin for column-purification, eliminating all steps of perfusion process and maintaining a sink condition of a toxic product in nutrient medium to optimize productivity of host cells. The instant invention also allows harvesting of solubilized inclusion bodies after the cells have been lysed and refolding of proteins inside the bioreactor.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: December 1, 2015
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz K. Niazi
  • Patent number: 9133493
    Abstract: The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.
    Type: Grant
    Filed: April 20, 2012
    Date of Patent: September 15, 2015
    Assignee: AMGEN INC.
    Inventors: Matthew I. Jerums, Amanda Kano, Henry Lin, Shun Luo, Jian Wu, Rebecca E. McCoy, Arvia E. Morris
  • Patent number: 9068215
    Abstract: A method of homogenously mixing the contents of a plurality of bioreactors by providing a receiving container capable of holding an appropriate quantity of the liquid and repeatedly raising and lowering the receiving container to a position above or below the position of the bioreactors resulting in mixing the contents of the bioreactors.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: June 30, 2015
    Assignee: Therapeutic Proteins International, LLC
    Inventor: Sarfaraz K. Niazi
  • Patent number: 9060915
    Abstract: Improving cell therapy and tissue regeneration in a patient suffering from a cardiovascular or a neurological disease by treating a tissue of the patient with shock waves and/or applying to the patient a therapeutically effective amount of stem cells and/or progenitor cells. Such treatment increases expression of chemoattractants, pro-angiogenic factors, and pro-survival factors. The chemoattractants can be, for example, vascular endothelial growth factor (VEGF) or stromal cell derived factor 1 (SDF-1). For example, the treated tissue can be located in the patient's heart or in a skeletal muscle of the patient, and the shock waves can be extracorporeal shock waves (ESW) or intracorporeal shock waves. The cardiovascular disease can have an ischemic or non-ischemic etiology. For example, the cardiovascular disease can be a myocardial infarction, ischemic cardiomyopathy, or a dilatative cardiomyopathy. For example, the neurological disease can be a peripheral neuropathy or neuropathic pain.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: June 23, 2015
    Assignee: Dornier MedTech Systems, GmbH
    Inventors: Andreas Lutz, Harald Eizenhofer, Andreas Michael Zeiher, Stefanie Dimmeler, Christopher Heeschen, Alexandra Aicher
  • Patent number: 9045733
    Abstract: A method of increasing the rate of growth, useful product production, or protein expression of a microorganism includes the step of exposing the microorganism to ultrasound having a frequency greater than about 1 MHz.
    Type: Grant
    Filed: August 26, 2009
    Date of Patent: June 2, 2015
    Assignee: Intelligentnano Inc.
    Inventors: Jie Chen, James Xing, Woon T. Ang
  • Patent number: 9040290
    Abstract: A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: May 26, 2015
    Assignee: Corning Incorporated
    Inventors: Gregory Roger Martin, Allison Jean Tanner
  • Publication number: 20150132845
    Abstract: Devices, systems, and methods for continuous cell culture and other reactions are generally described. In some embodiments, chambers (e.g., cell growth chambers) including at least a portion of a wall formed of a flexible member are provided. A retaining structure can be incorporated outside and proximate to the chamber such that when liquid is added to the chamber, the flexible member is consistently and predictably deformed, and a consistent volume of liquid is added. The flexible member can be formed of, in some embodiments, a gas-permeable medium. In some embodiments, reaction chambers can be arranged in a fluidic loop, and a bypass channel can be used to introduce and/or extract fluid from the loop without affecting loop operation.
    Type: Application
    Filed: November 6, 2014
    Publication date: May 14, 2015
    Applicant: Massachusetts Institute of Technology
    Inventors: Rajeev Jagga Ram, Kevin Shao-Kwan Lee
  • Publication number: 20150125428
    Abstract: A substantially enriched mammalian multipotent vascular stem cell (MVSC) population is provided, as well as compositions comprising the population. Methods are provided for the isolation, purification, and culture of the MVSCs. The MVSCs are useful in various applications, which are also provided.
    Type: Application
    Filed: January 29, 2013
    Publication date: May 7, 2015
    Applicant: The Regents of the University of California
    Inventors: Song Li, Zhenyu Tang, Aijun Wang
  • Patent number: 9023647
    Abstract: An improved monocyte activation test is described that is better able to detect non-endotoxin pyrogens in medical products, in which a sample is incubated with a monocyte-containing reagent in an assay system comprising at least one surface comprising polypropylene. The invention also concerns assay systems for use in these tests that include at least one microtiter well having at least one interior surface comprising polypropylene and having a shape such that monocyte-containing reagent is concentrated in the well to provide greater cell to cell contact. The invention also relates to a diagnostic kit that can be used to test for the presence of non-endotoxin pyrogens in a sample.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: May 5, 2015
    Assignees: Baxter International Inc., Baxter Healthcare SA, The Secretary of State for Health
    Inventors: Stephen Poole, Mehul Patel
  • Patent number: 9018007
    Abstract: The present invention relates to the use of cell cultures in the open continuous mode, to a method for selecting static cell variants or cell variants which proliferate in suspension, to a culture substrate and to a device suitable for implementing this method.
    Type: Grant
    Filed: February 20, 2009
    Date of Patent: April 28, 2015
    Assignee: Metabolium
    Inventors: Bernard Kudla, Pierre-Yves Chesneau, Yann Beaujouan
  • Patent number: 9005970
    Abstract: [PROBLEMS] Relating to a bioassay method with the use of cultured cells and cell culture for a therapeutic purpose in the case of determining the efficacy of a drug or the like or examining the toxicity thereof, it is intended to provide a method of culturing cells under regulation in the extension direction and a cell culture plate. [MEANS FOR SOLVING PROBLEMS] A cell culture plate having a plural number of side walls (1) and a plural number of space structures (3) for providing cultured cells which are formed by the side walls (1), wherein the side walls (1) are provided with openings (2) so that the space structures (3) are linked together. By using this cell culture plate, a method of culturing cells under regulation in the extension direction can be provided.
    Type: Grant
    Filed: January 11, 2006
    Date of Patent: April 14, 2015
    Assignees: Kuraray Co., Ltd., Ryosuke Nishio
    Inventors: Ryosuke Nishio, Taiji Nishi, Motohiro Fukuda, Yasuzo Kirita, Seiichi Kanai, Takenori Kitani
  • Patent number: 9005971
    Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber that is adapted to hold a fluid. A sparger is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparger. At least a portion of the sparger includes a sparging sheet formed from a flexible sheet of a gas permeable material. A tubular port or tube is coupled with the bag so that a passage bounded by the tubular port or tube communicates with the compartment.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: April 14, 2015
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones, Jeremy K. Larsen
  • Patent number: 9005967
    Abstract: The present invention provides a method for improving iPS cell generation efficiency, which comprises a step of introducing a Myc variant having the following features: (1) having an activity to improve iPS cell generation efficiency which is comparative to, or greater than that of c-Myc; and (2) having a transformation activity which is lower than that of c-Myc; or a nucleic acid encoding the variant, in a nuclear reprogramming step. Also, the present invention provides a method for preparing iPS cells, which comprises a step of introducing the above Myc variant or a nucleic acid encoding the variant and a combination of nuclear reprogramming factors into somatic cells. Moreover, the present invention provides iPS cells comprising the nucleic acid encoding the Myc variant which can be obtained by the above method, and a method for preparing somatic cells which comprises inducing differentiation of the iPS cells.
    Type: Grant
    Filed: January 21, 2011
    Date of Patent: April 14, 2015
    Assignee: Kyoto University
    Inventors: Shinya Yamanaka, Masato Nakagawa
  • Patent number: 8980630
    Abstract: The present invention relates to stem cells obtained from the amnion and their methods of obtaining and culturing. The present invention further relates to compositions comprising amnion-derived stems cells (ADSCs) and to methods of using ADSCs.
    Type: Grant
    Filed: June 28, 2007
    Date of Patent: March 17, 2015
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Dale Woodbury, Akiva J. Marcus
  • Patent number: 8980631
    Abstract: A method and device for creating hanging drop cell aggregates. The method and device includes a plurality of pegs that allow for high throughput culture of aggregates. Also disclosed are means of transferring formed aggregates to various destinations, such as well plates, scaffolding, tissues, or wounds. Use of the device permits aggregates to be prepared or created in larger quantities than current methods, and allows for them to be transferred more efficiently.
    Type: Grant
    Filed: June 30, 2010
    Date of Patent: March 17, 2015
    Assignee: University of Virginia Patent Foundation
    Inventors: Blair Taylor Stocks, Shayn Peirce Cottler, Adam J. Katz
  • Publication number: 20150072420
    Abstract: As described below, the present invention features compositions and methods related to the isolation, culture and therapeutic use of CD31-expressing cells.
    Type: Application
    Filed: April 18, 2014
    Publication date: March 12, 2015
    Inventors: Young-sup Yoon, Hyun-Jai Cho
  • Publication number: 20150056702
    Abstract: A method is provided, including adding a phthalide to a stem cell medium to provide a phthalide-containing medium, and then culturing a stem cell using the phthalide-containing medium. The use of a phthalide in a medium for culturing stem cells optionally maintains the pluripotency of stem cells. The phthalide also enhances the generation efficiency of induced pluripotent stem cells to decrease the culture cost.
    Type: Application
    Filed: October 31, 2014
    Publication date: February 26, 2015
  • Patent number: RE48523
    Abstract: A system and method of growing and harvesting algae provided whereby the system encompasses incubation tanks, internal lighting, chilled air diffusers, and an inline incubation tank for continuous batch processing. A centrifuge separates algae from growth media, and the media is processed through a series of reclamation steps so that cleaned water is reused for fresh media.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: April 20, 2021
    Inventor: Geronimos Dimitrelos