Support Is A Resin Patents (Class 435/396)
  • Patent number: 10317406
    Abstract: A system for detecting rare cells in a fluid is disclosed. The system includes a substrate and a mixture disposed on the substrate and including a carrier and a thermo-responsive polymer for capture and release of the rare cells. Also disclosed is a method for detecting rare cells in a fluid using a system including a substrate and a mixture that is disposed on the substrate. The mixture includes a carrier and a thermo-responsive polymer. The method includes providing the system and introducing a sample of fluid containing the rare cells into the system such that the sample interacts with the carrier for capturing the rare cells.
    Type: Grant
    Filed: April 6, 2016
    Date of Patent: June 11, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Hyeun Joong Yoon, Apoorv Shanker, Jinsang Kim, Sunitha Nagrath, Vasudha Murlidhar
  • Patent number: 10240122
    Abstract: A composition for producing a fiber, containing (A) a polymer compound containing a unit structure represented by the formula (1) and a unit structure represented by the formula (2), (B) a crosslinking agent, (C) an acid compound, and (D) a solvent wherein each symbol in the formulas (1) and (2) is as described in the DESCRIPTION.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: March 26, 2019
    Assignees: NISSAN CHEMICAL INDUSTRIES, LTD., KYOTO UNIVERSITY
    Inventors: Makiko Umezaki, Takahiro Kishioka, Taito Nishino, Ayako Otani, Kenichiro Kamei, Li Liu, Yong Chen
  • Patent number: 9950094
    Abstract: Provided are layered cell sheets, comprising a plurality of layered cell sheets containing myoblasts, in which each cell sheet comprises cell population containing myoblasts with controlled orientations. Preferably provided are the layered cell sheets comprising a region in which the orientations of the cell population containing the myoblasts in each cell sheet are identical to each other.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: April 24, 2018
    Assignee: Tokyo Women's Medical University
    Inventors: Hironobu Takahashi, Tatsuya Shimizu, Teruo Okano
  • Patent number: 9783637
    Abstract: A biodegradable electroactive material can be doped with a drug and the drug can be delivered to a living subject by stimulating the material with an electrical potential. The material (in this case a polymer) has an electrochemically responsive oligoaniline block terminated with a carboxylic acid moiety and is linked to an alcohol-terminated diol by an ester bond. Advantageously, the diol is PEG-400, PEG-2000, PCL-530, or PCL-2000.
    Type: Grant
    Filed: March 21, 2014
    Date of Patent: October 10, 2017
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: John Hardy, Christine E. Schmidt
  • Patent number: 9771410
    Abstract: Foam-formed collagen strands and methods for forming strands involve depositing a dispersed solution of an isolated cleaned, de-fatted, enzymatically-treated (or non-enzyme treated) human-derived collagen product having a preserved amount of its natural constituents into grooves of a grooved plate, and processing the dispersed collagen product to provide a foam-formed collagen strand. Foam-formed collagen strands may be processed into threads having a matrix of reticulated pores to conduct biological materials in and through the strand, the collagen of the collagen strand comprising isolated, enzymatically-treated human derived collagen having a preserved amount of its natural collagen constituents.
    Type: Grant
    Filed: July 17, 2014
    Date of Patent: September 26, 2017
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: Nels Lauritzen, Lawrence A. Shimp, Brent S. Mitchell
  • Patent number: 9376515
    Abstract: A production method for a vinyl ether polymer of the present technology is a production method for a vinyl ether polymer, wherein a vinyl ether monomer is subjected to living radical polymerization using a polymerization initiator, a monovalent copper compound, a ligand which is coordinated to the above copper compound, and ascorbic acid in a solvent. The above solvent has a mass ratio of isopropyl alcohol to water from 30:70 to 0:100. A mass ratio of the above vinyl ether monomer to the above solvent is from 10:100 to 25:100. A molar ratio of copper in the above copper compound to the above ascorbic acid is from 1:0.5 to 1:2.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: June 28, 2016
    Assignee: The Yokohama Rubber Co., LTD.
    Inventor: Takahiro Okamatsu
  • Patent number: 9279102
    Abstract: To form a temperature-responsive surface for cell culture by simple processes, said temperature-responsive surface for cell culture being capable of efficiently culturing cells. Cultured cells or a cell sheet can be efficiently removed from the temperature-responsive surface for cell culture by merely changing the temperature of the substrate surface. To coat the substrate surface with a block copolymer, in which a water insoluble polymer segment is coupled with a temperature-responsive polymer segment, in an amount of 0.8 to 3.0 ?g/cm2 of the temperature-responsive polymer.
    Type: Grant
    Filed: August 31, 2011
    Date of Patent: March 8, 2016
    Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
    Inventors: Masamichi Nakayama, Teruo Okano
  • Patent number: 9116144
    Abstract: A stimuli responsive nanofiber that includes a stimuli responsive polymer, such as a thermally responsive polymer, and a cross-linking agent having at least two latent reactive activatable groups. The nanofiber may also include a biologically active material or a functional polymer. The stimuli responsive nanofiber can be used to modify the surface of a substrate. When the nanofiber includes a thermally responsive polymer, the physical properties of the surface can be controlled by controlling the temperature of the system, thus controlling the ability of the surface to bind to a biologically active material of interest.
    Type: Grant
    Filed: December 10, 2012
    Date of Patent: August 25, 2015
    Assignee: INNOVATIVE SURFACE TECHNOLOGIES, INC.
    Inventors: Tahmina Naqvi, Jie Wen, Patrick Guire
  • Publication number: 20150147806
    Abstract: Disclosed is a method for preparing a transferable membrane having a nanometer scale dimension in thickness and pore size by non-solvent vapor-induced phase separation process, comprising spin-casting a polymer solution in a closed humid chamber and controlling the relative humidity (RH) of the chamber using at least one supersaturated salts solution whereby the density of the pores are controlled. Also provided is a TNT membrane prepared by the present method and its use. The present membrane can be advantageously used as co-culture platform facilitating versatile and controllable cell co-culture assays and further allowing the quantitative analysis of paracrine communications between cells for example between cancer cells and different types of stromal cells by providing an in vivo-like environment, which can offer more in-vivo-like results to identify key signaling molecules for therapeutic targets of a disease.
    Type: Application
    Filed: November 20, 2014
    Publication date: May 28, 2015
    Applicant: SNU R&DB FOUNDATION
    Inventors: Kookheon CHAR, Jwa-Min NAM, Yeongseon JANG, Hyojin LEE
  • Publication number: 20150148897
    Abstract: A method of forming a matrix of aligned nanofibres of elevated pore size and porosity comprises spraying a polymer solution towards a rotating drum so as to form nanofibres which are collected on the drum. The matrix can be used to form artificial tissue by removing the matrix from the drum, depositing cells onto the matrix and allowing the cells to form artificial tissue. Such artificial tissue finds use in the treatment of disease or damaged tissue, and in particular in the treatment of cardiovascular disease.
    Type: Application
    Filed: November 26, 2014
    Publication date: May 28, 2015
    Inventors: Jerome Jean-Luc SOHIER, Magdi Habib Yacoub
  • Publication number: 20150140659
    Abstract: A three-dimensional cell culture system for an imaging system to observe a cell image includes at least two cell culture layers formed by a solution having a photo-polymerizable monomer, a bio-molecule, an acoustic scattering medium solution and a cell culture medium. After placing a cell into the two cell culture layers, the two cell culture layers are laminated to form a three-dimensional culture laminating layer for culturing the cell. After forming the three-dimensional culture laminating layer, at least one cell-locating layer having a polyethylene glycol diacrylate (PEGDA) solution, the acoustic scattering medium solution, a plurality of photoacoustic markers and the cell culture medium is positioned into the three-dimensional culture laminating layer so as to form the three-dimensional cell culture system. The imaging system is constructed according to a theory selected from one of optics, acoustics, optoacoutics and acousto-optics.
    Type: Application
    Filed: March 13, 2014
    Publication date: May 21, 2015
    Applicant: NATIONAL TAIWAN UNIVERSITY
    Inventors: PAI-CHI LI, PO-LING KUO, CHIN-HSIUNG TSAI
  • Publication number: 20150140660
    Abstract: The present invention provides a process of coating at least a portion of a substrate surface comprising contacting the surface with hydrogen cyanide monomeric units under conditions permitting polymerisation of the hydrogen cyanide monomeric units to form a polymer that coats the surface. Also provided is a substrate coated by a polymer according to the claimed process. Also provided is a method of forming a hydrocyanic acid-based hydrogel, the method comprising co-polymerisation in a solution, the solution comprising hydrogen cyanide monomer units and co-monomers.
    Type: Application
    Filed: May 17, 2013
    Publication date: May 21, 2015
    Inventors: Helmut Thissen, Richard Evans, Aylin Koegler
  • Patent number: 9029149
    Abstract: Provided herein are apparatus and systems for fabricating highly aligned arrays of polymeric fibers having isodiameters ranging from sub 50 nm to microns with lengths of several millimeters. The approach disclosed herein uses (e.g.) a micropipette to deliver polymeric solution which is collected in the form of aligned fibers on a rotating and linearly translating substrate. The methods deposit polymeric fibers on spherical surfaces and gapped surfaces with precise control, thus heralding new opportunities for a variety of applications employing polymeric fibers. The design workspace for depositing fibers disclosed herein is dependent upon processing parameters of rotational/linear translational speeds and material properties of solution rheologies. Techniques for fabrication of multilayer fiber arrays, for fabrication of cell growth scaffolds and for attachment of particles to the fiber arrays are also disclosed.
    Type: Grant
    Filed: July 30, 2009
    Date of Patent: May 12, 2015
    Assignee: Carnegie Mellon University
    Inventor: Amrinder Singh Nain
  • Publication number: 20150125957
    Abstract: An elastomeric substrate comprises a surface with regions of heterogeneous rigidity, wherein the regions are formed by exposing the elastomeric substrate to an energy source to form the regions such that the regions include a rigidity pattern comprising spots.
    Type: Application
    Filed: October 24, 2014
    Publication date: May 7, 2015
    Inventors: Manus J.P. Biggs, Ryan Cooper, Jinyu Liao, Teresa Anne Fazio, Carl Fredrik Oskar Dahlberg, Jeffrey William Kysar, Shalom Jonas Wind
  • Publication number: 20150125952
    Abstract: The present invention generally relates to the field of cell growth and tissue engineering, in particular, tissue engineered compositions comprising a nanotextured substrate which is structurally configured for growth of cells in an anatomically correct adult phenotype in vitro. In particular, described herein are nanotextured substrates which are structurally configured for the anisotropic organization, maturation, and growth of in vitro-differentiated muscle cells, such as cardiomyocytes, and methods for the production and use thereof in varying sizes, nanotextures and substrate rigidities. In vitro-differentiated cardiomyocytes grown on the nanotextured substrates described herein are better-differentiated and more closely mimic adult cardiac tissue than the same cells grown on a non-textured substrate of the same composition.
    Type: Application
    Filed: March 15, 2013
    Publication date: May 7, 2015
    Inventors: Deok-Ho Kim, Michael Laflamme, Charles Murry, Kshitiz Gupta, Hyok Yoo, Alex Jiao
  • Publication number: 20150118200
    Abstract: Provided is a matrix for promoting survival and differentiation of cells transplanted thereon, comprising a base matrix and a cell-made matrix thereon. Methods and means for making and using same are also provided. Also provided are conditioned media, related compositions, related methods, and related packaging products.
    Type: Application
    Filed: December 24, 2014
    Publication date: April 30, 2015
    Inventors: Ilene SUGINO, Vamsi GULLAPALLI, Marco ZARBIN
  • Publication number: 20150118197
    Abstract: The invention provides a method for producing an electrospun scaffold, comprising electrospinning a polymer or co-polymer onto a template comprising a conductive collector having a three dimensional pattern thereon, wherein said electrospun polymer or co-polymer preferentially deposits onto said three dimensional pattern.
    Type: Application
    Filed: May 1, 2013
    Publication date: April 30, 2015
    Inventors: Frederik Claeyssens, Ilida Ortega, Sheila MacNeil, Anthony Ryan
  • Publication number: 20150112419
    Abstract: Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.
    Type: Application
    Filed: October 20, 2014
    Publication date: April 23, 2015
    Inventors: Hyunhee Ahn, Young Min Ju, Anthony Atala, Sang Jin Lee
  • Patent number: 9011754
    Abstract: The present invention refers to an apparatus and a method for the manufacture of a three-dimensional scaffold at low temperatures and the respective use of this method and apparatus.
    Type: Grant
    Filed: December 5, 2007
    Date of Patent: April 21, 2015
    Assignee: Nanyang Technological University
    Inventors: Meng Fatt Leong, Tze Chiun Lim, Kerm Sin Chian
  • Publication number: 20150087062
    Abstract: This invention relates a structure and system for growth factor incorporation which can improve the osteogenic differentiation of hMSCs, for potential bone regeneration and bone growth applications or used alone for bone repair or growth applications. The system comprises a biodegradable polyester, a hydrophilic polymer, a growth factor and optionally a bioceramic.
    Type: Application
    Filed: April 13, 2012
    Publication date: March 26, 2015
    Inventors: Treena Lynne Arinzeh, Tamunotonye Briggs
  • Publication number: 20150087057
    Abstract: There is provided a method for culturing a stem cell in vitro. The method comprises providing a substrate surface coated with a coating comprising a molecule having a catechol moiety or a polymer thereof; and growing a stem cell on said coated substrate surface in a growth medium.
    Type: Application
    Filed: April 22, 2013
    Publication date: March 26, 2015
    Inventors: Daniele Zink, Ming Ni, Karthikeyan Narayanan, Karthikeyan Kandasamy, Andrew C.A. Wan, Jackie Y. Ying
  • Publication number: 20150072429
    Abstract: The present invention relates to a method for manufacturing a three-dimensional (3D) biomimetic scaffold that exploits the use of electrical fields and electrical insulating materials to pattern previously polymerized hydro gels with different molecules and/or macromolecular entities. The invention also relates to the 3D-biomimetic scaffolds obtained and to the uses and applications thereof.
    Type: Application
    Filed: January 25, 2013
    Publication date: March 12, 2015
    Inventors: Alvaro Mata Chavarria, Juan Pablo Aguilar
  • Publication number: 20150072430
    Abstract: A structure for use in cell and tissue culturing and in other surface and interface applications. The structure comprises a first material layer defining one or more surface features therein disposed randomly or in a pattern, the one or more surface features having the same or different sizes and cross sectional shapes, a second material layer disposed in or on the one or more surface features, a microstructure disposed in or on the one or more surface features and at least partially embedded and immobile within the second material layer, the microstructure presenting a curvature and a stiffness value and protruding above an upper surface of the second material, a size of the microstructure between 1 nanometer and 10 millimeters, and the structure for use in cell and tissue culturing and in other surface and interface applications wherein a cell grows on the microstructure.
    Type: Application
    Filed: August 11, 2014
    Publication date: March 12, 2015
    Inventor: Shengyuan Yang
  • Publication number: 20150064146
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Application
    Filed: November 6, 2014
    Publication date: March 5, 2015
    Inventors: Ed Harlow, Roderick A. Hyde, Edward K.Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, JR.
  • Publication number: 20150050736
    Abstract: A hybrid tissue scaffold is provided which comprises a porous primary scaffold having a plurality of pores and a porous secondary scaffold having a plurality of pores, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold. The pores of the porous primary scaffold may have a pore size in a range of 0.50 mm to 5.0 mm, and the pores of the porous secondary scaffold may have a pore size in a range of 50 ?m to 600 ?m. The primary scaffold may provide 5% to 30% of a volume of the hybrid scaffold.
    Type: Application
    Filed: August 25, 2014
    Publication date: February 19, 2015
    Inventors: Jeffrey N. HARRIS, Jian LING, Xingguo CHENG
  • Patent number: 8956638
    Abstract: A method is provided for the preparation of a poly(amic acid) in which ring opening polymerization is employed to react the monomers ethylenediaminetetraacetic dianhydride and paraphenylenediamine in an aprotic solvent. The resulting poly(amic acid) composition is suitable as a biocompatible material, such as a biomedical implant, implant coating material, tissue scaffold material, controlled release drug delivery vehicle, and cellular growth substrate.
    Type: Grant
    Filed: April 8, 2011
    Date of Patent: February 17, 2015
    Assignee: Axcelon Biopolymers Corporation
    Inventors: Wankei Wan, Donna Padavan
  • Publication number: 20150037884
    Abstract: A tissue engineered construct made totally or in part from biocompatible materials and mammalian cells and/or cell products is provided. These constructs are useful in regenerating complex tissues such as bone, ligament and tendon, which may fabricated into medical devices suitable for use in the treatment of injuries and maladies such as rotator cuff injuries, periodontal disease and hernia.
    Type: Application
    Filed: October 21, 2014
    Publication date: February 5, 2015
    Inventors: Anthony Ratcliffe, Andreas Kern, Fatemeh Ratcliffe
  • Publication number: 20150031131
    Abstract: The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs.
    Type: Application
    Filed: September 24, 2014
    Publication date: January 29, 2015
    Inventors: Shannon L.M. Dahl, Laura E. Niklason, Justin T. Strader, William E. Tente, Joseph J. Lundquist
  • Publication number: 20150030658
    Abstract: The present invention relates to biocompatible compositions for transplantation into a sub-retinal space of the human eye. The compositions include a biodegradable polyester film, preferably a polycaprolactone (PCL) film, and a layer of human retinal progenitor cells. The compositions of the invention can be used as scaffolds for the treatment a number of ocular diseases, including retinitis pigmentosa and age-related macular degeneration.
    Type: Application
    Filed: January 22, 2013
    Publication date: January 29, 2015
    Inventors: Caio Regatieri, Petr Y. Baranov, Michael J. Young
  • Publication number: 20150024967
    Abstract: Provided herein is a three-dimensional scaffold composition comprising randomly oriented fibers, wherein the fibers comprise a polyethylene glycol-polylactic acid block copolymer (PEG-PLA) and a poly(lactic-co-glycolic acid) (PLGA). Also provided are methods for using the three-dimensional scaffolds described herein.
    Type: Application
    Filed: July 31, 2014
    Publication date: January 22, 2015
    Applicant: University of South Florida (A Florida Non-Profit Corporation)
    Inventors: Subhra Mohapatra, Shyam S. Mohapatra
  • Publication number: 20150024493
    Abstract: A method for preparing a patterned substrate is provided. The method includes melt-spinning at least one biocompatible polymer to form fibers; collecting the fibers on a substrate such that the fibers are aligned on the substrate; and applying a binding agent to the aligned fibers to bond the fibers into the aligned arrangement to obtain the patterned substrate in form of an aligned fiber mat. Use of the patterned substrate in an implant for tissue engineering is also provided.
    Type: Application
    Filed: July 15, 2014
    Publication date: January 22, 2015
    Inventors: Subramanian Venkatraman, Scott Alexander Irvine, Chee Kai Chua, Animesh Agrawal, Jia An
  • Publication number: 20150024494
    Abstract: In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
    Type: Application
    Filed: October 9, 2014
    Publication date: January 22, 2015
    Inventors: SUPARNA SANYAL, DEEPA SAXENA, SUSAN XIUQI QIAN, ELIZABETH ABRAHAM
  • Publication number: 20150017725
    Abstract: A polyurethane porous membrane is produced by a simple method to be used for at least one of applications of cell culture and cancer cell growth inhibition. The production method of the polyurethane porous membrane to be used for at least one of the applications of cell culture and cancer cell growth inhibition comprises: a first step of forming a layer of a polyurethane material which is uncured, on a substrate; and a second step of supplying water vapor to an exposed surface of the layer of the polyurethane material formed on the substrate, which is away from the substrate, so as to cure the polyurethane material and provide the layer of the polyurethane material with a porous structure having a plurality of irregularities on the exposed surface.
    Type: Application
    Filed: July 1, 2014
    Publication date: January 15, 2015
    Inventors: Seitaro TAKI, Hisashi MIZUNO, Hiroyuki NAKAGAWA, Toshiyuki HAGIYAMA, Atsuki YOSHIMURA, Masaru TANAKA, Ayano SASAKI, Toshifumi TAKAHASHI, Tsuyoshi OHTA
  • Publication number: 20150010999
    Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.
    Type: Application
    Filed: January 31, 2013
    Publication date: January 8, 2015
    Inventors: Stephen Joseph Caracci, David Henry, Jessica Jo Kelley, Mark Alan Lewis, Yue Zhou
  • Publication number: 20140377863
    Abstract: A synthetic scaffold for replacing at least a portion of an airway includes an airway mold, one or more structural ribs on the airway mold, and a non-structural wall. Each of the one or more structural ribs is formed from a first material and the non-structural wall is formed from a second material. The non-structural wall coats the airway mold and forms a conduit that incorporates the one or more structural ribs.
    Type: Application
    Filed: September 11, 2014
    Publication date: December 25, 2014
    Inventors: Alexander M Seifalain, Paolo Macchiarini
  • Publication number: 20140377213
    Abstract: A cell growth matrix is provided that comprises a biodegradable elastomeric polymer electrodeposited concurrently with a sprayed or electrosprayed liquid that is a physiological solution or which comprises a mammalian blood product such as serum, plasma or platelet rich plasma. The matrix is useful as a cell-growth matrix and for repair of a tissue in a mammal, for instance by implantation in a mammal at a site in need of repair, such as in an abdominal wall.
    Type: Application
    Filed: May 27, 2011
    Publication date: December 25, 2014
    Applicant: UNIVERSITY OF PITTSBURGH - OF THE COMMONWEALTH SYSTEM OF HIGHER EDUCATION
    Inventors: Yi Hong, Nicholas J. Amoroso, Kazuro Lee Fujimoto, Ryotaro Hashizume, Michael Steven Sacks, William R. Wagner
  • Patent number: 8916382
    Abstract: In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: December 23, 2014
    Assignee: Corning Incorporated
    Inventors: Suparna Sanyal, Deepa Saxena, Susan Xiuqi Qian, Elizabeth Abraham
  • Publication number: 20140356949
    Abstract: A carrier for expansion of induced pluripotent stem cells is provided, wherein the carrier comprises a substrate comprising one or more outer surfaces, wherein the one or more outer surfaces are modified with gas plasma treatment, and one or more structured indentations on one or more of the outer surfaces. The carrier has a length at least about 0.2 mm, a width at least about 0.2 mm, and a height in a range from about 0.05 mm to 1.2 mm and each of the structured indentations has a major axis in a range from about 0.1 mm to 0.5 mm, a minor axis in a range from about 0.1 mm to 0.5 mm and a depth in a range from about 0.025 mm to about 0.5 mm. A method of making the carrier, and culturing induced pluripotent stem cells using the same carrier are also provided.
    Type: Application
    Filed: August 18, 2014
    Publication date: December 4, 2014
    Inventors: Brian Michael Davis, Kenneth Roger Conway, Evelina Roxana Loghin, Andrew Arthur Paul Burns, David Gilles Gascoyne, Scott Michael Miller
  • Patent number: 8889415
    Abstract: A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.
    Type: Grant
    Filed: April 30, 2007
    Date of Patent: November 18, 2014
    Inventor: Ray Jui-Fang Tsai
  • Patent number: 8883503
    Abstract: Disclosed herein are biodegradable hydrogel scaffolds for use in tissue engineering. The hydrogel scaffolds are composed of synthetic terpolymers complexed with polyvinyl alcohol (PVA), which facilitate cell-sheet and tissue growth. In the presence of a monosaccharide, the PVA-hydrogel is dissolved and cell-sheets are released for harvesting. Further disclosed herein are methods for producing PVA hydrogels which support tissue growth. Tissue engineering applications and methods are also disclosed.
    Type: Grant
    Filed: August 8, 2011
    Date of Patent: November 11, 2014
    Assignee: Indian Institute of Technology Kanpur
    Inventor: Ashok Kumar
  • Publication number: 20140330392
    Abstract: The present invention provides tissue scaffolds, methods of generating such scaffolds, and methods of use of such scaffolds to generate aligned and functional tissues for use in methods including regenerative medicine, wound repair, and transplantation.
    Type: Application
    Filed: December 6, 2012
    Publication date: November 6, 2014
    Applicant: THE TRUSTEES OF PRINCETON UNIVERSITY
    Inventors: Jeffrey Schwartz, Jean E. Schwarzbauer, Casey M. Jones, Patrick E. Donnelly, Stephen B. Bandini, Shivani Singh
  • Publication number: 20140328789
    Abstract: A pH-responsive cell scaffold for growing a cell culture is disclosed. The cell scaffold has pores in which biological cells may be disposed. As the pH of the local environment drops, the cell scaffold swells to draw in additional oxygen and/or other nutrients. The increased supply of oxygen and/or nutrients increases the longevity of the cells. In some embodiments, the cell scaffold induces a change in gene expression that promotes wound healing.
    Type: Application
    Filed: May 5, 2014
    Publication date: November 6, 2014
    Inventors: Debra T. Auguste, Jin-Oh You
  • Publication number: 20140326391
    Abstract: This invention provides a cost-effective technique for mass-production of a cell culture vessel that allows mass cell culture to be performed in a manner that allows cells to be stably detached from the culture vessel. Specifically, the invention provides a method for producing a cell culture vessel suitable for large-capacity culture comprising cutting a long-sized cell support film to obtain a sheet-like cell support film, fixing the film to a first member, which is not closed, and bonding other members thereto. Thus, a cell culture vessel comprising a container section in which a cell support film is fixed to an inner wall surface can be produced.
    Type: Application
    Filed: October 29, 2012
    Publication date: November 6, 2014
    Applicant: Dai Nippon Printing Co., Ltd.
    Inventors: Masatoshi Kuroda, Katsunori Tsuchiya, Masahiko Hase, Taro Nagai, Yumiko Narita, Kazumasa Yamaki
  • Patent number: 8877496
    Abstract: The invention provides a method for transferring cells to carriers, including: (a) providing a hydrophobic cell culture container or a cell culture container coated with a hydrophobic material on a bottom thereof; (b) adding carriers which are more hydrophilic than the hydrophobic cell culture container or hydrophobic materials and a culture medium containing cells into the hydrophobic cell culture container or the cell culture container coated with the hydrophobic material on the bottom thereof; and (c) culturing the cells, wherein the cells attach to the carriers and grow.
    Type: Grant
    Filed: June 5, 2009
    Date of Patent: November 4, 2014
    Assignee: National Taiwan University
    Inventors: Chin-Hsiung Hsieh, Yi-You Huang
  • Patent number: 8877499
    Abstract: A biocompatible implantable bone anchor is provided that has a threaded first portion that engages and anchors into a bone. The implant also has a neck region extending from the first portion adapted to promote autologous cell growth thereon at an interface of the bone and one or more epidermal or gum layers, the neck region having a plurality of channels extending about the neck region. The neck region is configured to mechanically engage at least one of an abutment, dental restoration, or osseous device attachment. An in situ bone anchor cell growth assembly includes the bone anchor and a manifold encompassing the neck portion so as to form a seal therebetween and a route of fluid communication between a manifold inlet and at least one of said plurality of channels. A process for growing autologous cells on a neck region of a bone anchor is provided.
    Type: Grant
    Filed: January 31, 2013
    Date of Patent: November 4, 2014
    Assignee: ViaDerm LLC
    Inventors: Allen B. Kantrowitz, Michael N. Helmus
  • Patent number: 8877223
    Abstract: The present application discloses biodegradable polymers, to porous and other materials comprising such polymers, and to various medical uses of such materials, including use as a scaffold for supporting cell adhesion or the in-growth for regeneration of tissue. The polymer is of the formula A-O—(CHR1CHR2O)n—B wherein A is a poly(lactide-co-glycolide) residue, the molar ratio of (i) lactide units [—CH(CH3)—COO—] and (ii) glycolide units [—CH2—COO—] in the poly(lactide-co-glycolide) residue being in the range of 80:20 to 10:90, B is either a poly(lactide-co-glycolide) residue or hydrogen, C1-6-alkyl or hydroxy protecting groups, one of R1 and R2 is hydrogen or methyl, and the other is hydrogen, n is 10-1000, the molar ratio of (iii) polyalkylene glycol units [—(CHR1CHR2O)—] to the combined amount of (i) lactide units and (ii) glycolide units in the poly(lactide-co-glycolide) residue(s) is at the most 14:86, and the molecular weight of the copolymer is at least 20,000 g/mol.
    Type: Grant
    Filed: March 7, 2007
    Date of Patent: November 4, 2014
    Assignee: Coloplast A/S
    Inventors: Jakob Vange, Khadija Schwach-Abdellaoui, Hanne Everland, Peter Sylvest Nielsen, Brian Nielsen, Lene Karin Jespersen, Lene Feldskov Nielsen
  • Publication number: 20140322786
    Abstract: Provided are polymer modified substrates which comprise a) a substrate, b) a binding layer covalently attached to the surface of the substrate and covering at least a part of this surface; and c) a polymer brush formed by a plurality of polymer chains, each of which is covalently attached at one of its terminals to the binding layer. Moreover, methods are provided, for the preparation of the polymer modified substrates by polymerizing vinyl group containing monomers, such as vinylphosphonates, on a binding layer provided on a substrate.
    Type: Application
    Filed: November 13, 2012
    Publication date: October 30, 2014
    Applicant: APCETH GMBH & CO KG
    Inventors: Frank Deubel, Bernhard Rieger, Stephan Salzinger, Ning Zhang
  • Patent number: 8871512
    Abstract: Sugar-acrylic monomers are synthesized to have a carbohydrate moiety linked to an acrylate group. The sugar-acrylic monomers may be polymerized to form polymers, adhesives, hydrogels, and the like. The sugar-acrylic monomers and polymers may be used in tissue engineering, adhesives and sealers, wound healing, and the like.
    Type: Grant
    Filed: October 27, 2010
    Date of Patent: October 28, 2014
    Assignee: Empire Technology Development LLC
    Inventors: William B. Carlson, Gregory D. Phelan, Phillip A. Sullivan
  • Publication number: 20140287506
    Abstract: In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.
    Type: Application
    Filed: May 19, 2014
    Publication date: September 25, 2014
    Applicant: Corning Incorporated
    Inventors: Suparna Sanyal, Deepa Saxena, Susan Xiuqi Qian, Elizabeth Abraham
  • Publication number: 20140273223
    Abstract: Disclosed is micro-device for culturing cells comprising: a plurality of fluid paths through which fluid moves; and at least one inlet port for injecting fluid to the fluid paths, said fluid paths communicating with each other and being different in height from each other. In the cell culture device having a plurality of fluid paths, cells can be cultured by introducing a polymeric material to at least one fluid path having a relatively low height; solidifying the polymeric material to form a 3-dimensional scaffold; and injecting fluid for cell culture to a fluid path in contact with the 3-dimensional scaffold.
    Type: Application
    Filed: September 29, 2011
    Publication date: September 18, 2014
    Applicant: UNIST ACADEMY-INDUSTRY RESEARCH CORPORATION
    Inventors: Yoon-Kyoung Cho, Hyundoo Hwang