Solid Support And Method Of Culturing Cells On Said Solid Support Patents (Class 435/395)
  • Patent number: 11396537
    Abstract: The present invention relates to a polypeptide, a process for the production thereof and a use thereof. The polypeptide of the present invention has excellent adhesion effect and is highly stable in an aqueous solution. Endotoxins can be more easily removed from the product of the polypeptide.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: July 26, 2022
    Assignee: Shanxi Jinbo Bio-Pharmaceutical Co., Ltd.
    Inventor: Xia Yang
  • Patent number: 11390842
    Abstract: A cell culture apparatus for conducting cell culture in which a culture medium is supplied together with cells to a container formed of a flexible material, wherein, a base having a flat mounting surface that holds the container is provided, partition pieces that can be protruded from the mounting surface for a prescribed height are embedded in the base, and when the partition pieces are accommodated into the base, upper end surfaces of the partition pieces are flushed with the mounting surface.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: July 19, 2022
    Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
    Inventors: Norihiko Hata, Masahiro Murai
  • Patent number: 11376347
    Abstract: Provided are elastin-rich bioscaffolds, enhanced via stem cell pre-seeding and flow-based mechanical conditioning followed by subsequent removal of stem cells. The bioscaffolds may accelerate cardiovascular tissue regeneration and can be used in the surgical repair/reconstruction in various cardiovascular and other surgical applications including heart valves, cardiovascular grafts, and patches. The provided acellular, elastin-rich bioscaffold will pose minimal immune risk and reduced FDA regulatory hurdles as a non-living biologic product.
    Type: Grant
    Filed: June 21, 2021
    Date of Patent: July 5, 2022
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Sharan D. Ramaswamy, Brittany Gonzalez, Ariadna Herrera, Alexander Williams
  • Patent number: 11345841
    Abstract: A fiber silica composite microsphere for a shale stratum comprises a hollow silica sphere and fibers, and the fibers are partially coated on an outer surface of the hollow silica sphere and partially embedded into an interior of the hollow silica sphere. The hollow silica sphere has an outer diameter of 1-5 ?m and an inner diameter of 0.8-4.7 ?m; the fibers have a length of 5-10 ?m and a width of 1-3 ?m.
    Type: Grant
    Filed: October 19, 2021
    Date of Patent: May 31, 2022
    Assignee: Southwest Petroleum University
    Inventors: Yang Bai, Lingfeng Wu, Pingya Luo, Wenzhe Li, Feng Dai, Gang Xie, Haibo Liang, Kesheng Rong, Jiading Deng
  • Patent number: 11318224
    Abstract: Coated and expanded, nanofiber structures are provided and methods of use thereof.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: May 3, 2022
    Assignee: BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA
    Inventors: Jingwei Xie, Shixuan Chen, Mark Carlson
  • Patent number: 11154409
    Abstract: A percutaneous collar is made up of a central rigid ring and a flexible mesh inside a microporous silicone disc. The volume of the disc has a three-dimensional network of interconnected micropores forming microchannels connecting both external faces of the disc through the external micropores to the internal flexible mesh wherein the flexible mesh is formed by crossed longitudinal and radial elements or plates which form a plurality of holes.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: October 26, 2021
    Assignees: UNIVERSITAT POLITÉCNICA DE VALÈNCIA, UNIVERSITAT DE VALÈNCIA, FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL CLÍNICO DE LA COMUNIDAD VALENCIANA (INCLIVA)
    Inventors: José Expósito Ollero, Ana Vallés Lluch, José Albelda Vitoria, Juan Victor Hoyos Fuentes, Antonio Silvestre Muñoz
  • Patent number: 11149252
    Abstract: The present disclosure aims to provide a method of efficiently manufacturing a cell mass, a cell structure, or a three-dimensional tissue body using a culturing surface coated with a temperature-responsive polymer or a temperature-responsive polymer composition. The manufacturing method of a cell mass, a cell structure, or a three-dimensional tissue body of the present disclosure includes seeding and culturing cells on a culturing surface coated with a temperature-responsive polymer or a temperature-responsive polymer composition.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: October 19, 2021
    Assignee: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER
    Inventors: Yasuhide Nakayama, Ryosuke Iwai, Yasushi Nemoto
  • Patent number: 11149241
    Abstract: Cell culture devices, and related methods and kits, for modeling isotropic-to-anisotropic cellular transitions are provided. The devices can include a substrate having an isotropic film surface with one or more regions of aligned fibers dispersed thereon.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: October 19, 2021
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Christopher John Rivet, Gregory Patrick Desmond, Jonathan Michael Zuidema, Ryan James Gilbert
  • Patent number: 11135589
    Abstract: Large bioreactors based on microfluidic technology, and methods of manufacturing the same, are provided, The big microbioreactor can include a chip or substrate having the microfluidic channels thereon, and the chip can be manufactured by forming a master mold, forming a male mold from a photopolymer plate using replica molding with the Fmold, and transferring features of the male to a polymer material.
    Type: Grant
    Filed: December 22, 2020
    Date of Patent: October 5, 2021
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Shekhar Bhansali, Maximiliano S. Perez, Betiana Lerner, Natalia Bourguignon
  • Patent number: 11046931
    Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: June 29, 2021
    Assignee: Coming Incorporated
    Inventors: Stephen Joseph Caracci, David Henry, Jessica Jo Kelley, Mark Alan Lewis, Yue Zhou
  • Patent number: 10920190
    Abstract: The present disclosure provides methods and systems for generating biological tissues that are configured for folding into a pre-determined three-dimensional form. The present disclosure utilizes contractile cells for folding a biological tissue into a three-dimensional shape. The methods include disposing a pattern of contractile cells on a surface that includes fibers actuated by the contractile cells and folding of the surface by the action of the contractile cells on the fibers. Tissues generated using the methods and systems of the present disclosure are also provided.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: February 16, 2021
    Assignee: The Regents of the University of California
    Inventors: Zev Jordan Gartner, Alex James Hughes
  • Patent number: 10875891
    Abstract: Disclosed herein are synthetic peptides and compositions comprising the same for the treatment and/or prophylaxis of a disease or a condition related to dry eye syndrome, psoriasis vulgaris or multiple myeloma. Also disclosed herein are methods of treating and/or preventing a disease or a condition related to dry eye syndrome, psoriasis vulgaris or multiple myeloma, by administering to a subject in need of such treatment a composition containing a therapeutically effective amount of a synthetic peptide of the present disclosure.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: December 29, 2020
    Assignee: MACKAY MEMORIAL HOSPITAL
    Inventors: Yeou-Ping Tsao, Tsung-Chuan Ho
  • Patent number: 10860001
    Abstract: A method of designing and manufacturing a replica composite object based on an original object. The method identifies the structure and physical properties of an original object. Base materials, bodies, and structural templates, each of which includes associated physical properties, are utilized to generate a 3-dimensional model. The 3-dimensional model is discretized and tested to determine if the selected combination of base materials and bodies have physical properties that substantially equal the physical properties of the original object. If the physical properties do not equate, the 3-dimensional model is optimized by adjusting the combination of base materials, bodies, and structural templates. When the difference between the measured physical properties of the 3-dimensional model and the identified physical properties of the original object is less than a tolerance value, the method instructs an additive manufacturing system to generate a replica composite object based on the original object.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: December 8, 2020
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: Fluvio Lobo Fenoglietto, Jack Stubbs
  • Patent number: 10836987
    Abstract: A microfluidic device for determining a response of cells comprises a microchannel and a seeding channel. The microchannel is at least partially defined by a porous membrane having cells adhered thereto. The microchannel has a first cross-sectional area. The seeding channel delivers a working fluid to the cells within the microchannel. The seeding channel has a second cross-sectional area that is less than the first cross-sectional area such that a flow of the working fluid produces a substantially higher shear force within the seeding channel to inhibit the attachment of cells within the seeding channel. And when multiple seeding channels are used to deliver fluids to multiple microchannels that define an active cellular layer across the membrane, the seeding channels are spatially offset from each other such that fluid communication between the fluids occurs only at the active region via the membrane, not at the seeding channels.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: November 17, 2020
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Karel Domansky, Christopher David Hinojosa, Donald E. Ingber, Daniel Levner, Guy Thompson, II
  • Patent number: 10829726
    Abstract: The pressure and circulation culture apparatus includes a holder (culture chamber part), a pressure transmission part and a culture solution circulation line (culture circuit). The holder is a means that reserves culture solution and that holds a cultured object. The pressure transmission part is a means that communicates with the holder and transmits an external pressure to the culture solution. The culture solution circulation line is a means that is connected to the holder via the pressure transmission part, and circulates the culture solution through the holder via the pressure transmission part. Such a structure is included, and an external pressure is made to operate on the culture solution via the pressure transmission part to allow pressure on the cultured object in the holder and circulation of the culture solution in the holder.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: November 10, 2020
    Assignee: PURPOSE CO., LTD.
    Inventors: Setuo Watanabe, Toshimi Murata, Mituharu Satou, Ibuki Kinouchi, Hidetada Takai
  • Patent number: 10793819
    Abstract: A liquid injection method for injecting a liquid into a culture vessel includes tilting the culture vessel around a horizontal axis at a tilt angle (X°) of greater than 0° and 50° or less, wherein adherent cells are adhered to the culture vessel; and injecting the liquid into the culture vessel at a predetermined linear velocity (Y mm/s) via a wall surface of the culture vessel tilted at the tilt angle (X°), wherein the tilt angle (X) and the linear velocity (Y) satisfy the following (formula 1): Y?5.075X+123 (formula 1).
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: October 6, 2020
    Assignees: KANEKA CORPORATION, KYOTO UNIVERSITY
    Inventors: Tomohisa Kato, Kazuya Hamada, Haruhisa Inoue, Takayuki Kondo
  • Patent number: 10732172
    Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.
    Type: Grant
    Filed: May 22, 2016
    Date of Patent: August 4, 2020
    Assignees: President and Fellows of Harvard College, Children's Medical Center Corporation
    Inventors: Donald E. Ingber, Abhishek Jain, Andries D. van der Meer, Alan David Michelson, Andrew L. Frelinger, III, Riccardo Barrile
  • Patent number: 10702627
    Abstract: This invention provides aragonite- and calcite-based scaffolds for the repair, regeneration, enhancement of formation or a combination thereof of cartilage and/or bone, which scaffolds comprise at least two phases, wherein each phase differs in terms of its chemical content, or structure, kits comprising the same, processes for producing solid aragonite or calcite scaffolds and methods of use thereof.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: July 7, 2020
    Assignee: Cartiheal (2009) Ltd.
    Inventors: Nir Altschuler, Razi Vago
  • Patent number: 10668467
    Abstract: A microfluidic device includes a substrate, a cover layer and one or more chambers disposed within the cover layer, the substrate or both. Each chamber has a first end, a second end, and a set of micro-pillars disposed therein. A first microchannel and second microchannel are disposed within the cover layer, the substrate or both, and connected to the first end and second end of the chamber, respectively. A first set of barriers is disposed within each first microchannel proximate to the first end of the chamber. A second set of barriers is disposed within each second microchannel proximate to the second end of the chamber. A third microchannel is disposed within the cover layer, the substrate or both, and connected to the chamber. A first port, second port and third port extend through the cover layer and connect to the first, second and third microchannels, respectively.
    Type: Grant
    Filed: June 7, 2016
    Date of Patent: June 2, 2020
    Assignee: Texas Tech University System
    Inventors: Siva A. Vanapalli, Mizanur Rahman
  • Patent number: 10533158
    Abstract: The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.
    Type: Grant
    Filed: February 28, 2006
    Date of Patent: January 14, 2020
    Assignees: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, CELLSEED, INC.
    Inventors: Masato Sato, Hideaki Sakai
  • Patent number: 10071187
    Abstract: Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: September 11, 2018
    Assignees: Cook Biotech Incorporated, Muffin Incorporated
    Inventors: Michael C. Hiles, Chad E. Johnson, Neal E. Fearnot, Thomas Payne, Ronald Jankowski
  • Patent number: 10004830
    Abstract: The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells (Human mesenchymal stem cells, Human fibroblast, Keratinocytes, fibroblast differentiated form mesenchymal stem cells in vitro and keratinocytes differentiated form mesenchymal stem cells in vitro) in vitro for continued periods of time. In the present invention, mesenchymal stem cells (MSCs) derived from bone marrow or umbilical cord are inoculated and grown on a pre-established scaffold or matrix made up of chitosan and gelatin. The developed tissue constructs contain cells, growth factors, cytokines and other regulatory factors secreted by cells. The developed bioengineered tissue constructs provide the support, growth factors and essential cytokines for wound healing. Developed tissue construct useful in the treatment of burns wound, diabetic foot ulcer wound and type of chronic skin wounds. It is helpful in healing and regeneration of skin tissue.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: June 26, 2018
    Assignee: DATT MEDIPRODUCTS LIMITED and DATT LIFE SCIENCE PVT. LTD.
    Inventors: Rajan Datt, Siddharth Pandey, Pallavi Shrivastava, Supriya Srivastava
  • Patent number: 9962464
    Abstract: Provided are injectable, hyaluronic acid-based dermal filler compositions including conjugated vitamin C derivatives. The compositions provide for extended release of active vitamin C into tissue, promoting collagenesis and other benefits to skin.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: May 8, 2018
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Jessamine P. Winer-Jones, Nicholas J. Manesis, Hengli Liu, Gabriel Njikang, Athene Wan Chie Chan
  • Patent number: 9913700
    Abstract: The present invention relates to a biomimetic matrix for providing structural support and scaffolding that allows for regeneration of dentin, pulp, and periodontal tissues. A method of making the biomimetic matrix provides the ability to select both a size of a pore or tubule formed in the biomimetic matrix and a density of pores or tubules disposed throughout the biomimetic matrix. The present invention discloses an approach of successful tubular dentin regeneration both in vitro and in vivo using the biomimetic matrix.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: March 13, 2018
    Assignee: The Texas A&M University System
    Inventors: Xiaohua Liu, Chi Ma, Tiejun Qu
  • Patent number: 9873861
    Abstract: The invention relates to implantable collagen devices made by seeding at least one elongate collagen construct, e.g., comprising at least one elongate synthetic collagen fiber with a plurality of cells and applying a strain and/or stress to the at least one elongate collagen fiber to induce the cells to differentiate into target phenotypes, e.g., tendon or ligament phenotype cells (and/or fibroblasts), typically with an extracellular matrix of collagen to organize into a tissue on the at least one collagen fiber.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: January 23, 2018
    Assignee: MiMedx Group, Inc.
    Inventor: Thomas J. Koob
  • Patent number: 9814802
    Abstract: A method of differentiating cells into CK19-positive cells capable of producing hair follicle-like and hair structure-like can include: providing a tissue scaffold; seeding cells into the scaffold, the cells being capable of differentiation; incubating the scaffold having the cells in a cell growth media; and incubating the scaffold having the cells in an osteogenic differentiation medium sufficient for CK19-positive cells to be generated in the scaffold. The tissue scaffold can be a decellularized Whartons' jelly matrix. The cell growth media excludes osteogenic differentiation components: dexamethasone, ?-glycerophosphate, 1?,25-hydroxyvitamin D3, and ascorbic acid 2-phosphate. The osteogenic differentiation medium includes the osteogenic differentiation components. The cells can be mesenchymal cells, such as WJMSCs.
    Type: Grant
    Filed: April 30, 2013
    Date of Patent: November 14, 2017
    Assignees: The University of Kansas, The Children's Mercy Hospital
    Inventors: Omar Aljitawi, Richard Hopkins, Michael Detamore, Rama Garimella
  • Patent number: 9777326
    Abstract: A method of sequencing nucleic acids, which can include steps of (a) providing a substrate comprising a surface having a repeating pattern of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the repeating pattern of features with a solution of different nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, and a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution; (c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the seeded subset; (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of different nucleic acid colonies; and (e) detecting sequencing reactions at the different nucleic acid colonies on the surface.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: October 3, 2017
    Assignee: ILLUMINA, INC.
    Inventors: Mostafa Ronaghi, Helmy A. Eltoukhy
  • Patent number: 9752112
    Abstract: The pressure and circulation culture apparatus includes a holder (culture chamber part), a pressure transmission part and a culture solution circulation line (culture circuit). The holder reserves culture solution and holds a cultured object. The pressure transmission part communicates with the holder and transmits an external pressure to the culture solution. The culture solution circulation line is connected to the holder via the pressure transmission part, and circulates the culture solution through the holder via the pressure transmission part. Such a structure is included, and an external pressure is made to operate on the culture solution via the pressure transmission part to allow pressure on the cultured object in the holder and circulation of the culture solution in the holder.
    Type: Grant
    Filed: February 23, 2012
    Date of Patent: September 5, 2017
    Assignee: PURPOSE CO., LTD.
    Inventors: Setuo Watanabe, Toshimi Murata, Mituharu Satou, Ibuki Kinouchi, Hidetada Takai
  • Patent number: 9752116
    Abstract: A composition comprising a plurality of cell aggregates for use in the production of engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension to form a pellet, extruding the pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing processes parameters for use in producing an engineered tissue having a desired three-dimensional structure.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: September 5, 2017
    Assignees: The Curators of the University of Missouri, MUSC Foundation for Research Development
    Inventors: Gabor Forgacs, Karoly Jakab, Adrian Neagu, Vladimir Mironov
  • Patent number: 9744041
    Abstract: A three component system for repairing critically sized bone defects having a first shape memory polymer (SMP) component formed as a scaffold that fills the defects, a second SMP component formed as a restricting sleeve that stabilizes and supports osseointegration and osteoconduction, and a third SMP component formed as a two-dimensional cell culture substrate for engineering periosteal grafts.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: August 29, 2017
    Assignee: Syracuse University
    Inventors: James Henderson, Patrick T. Mather
  • Patent number: 9745550
    Abstract: Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: August 29, 2017
    Assignee: Asterias Biotherapeutics, Inc.
    Inventors: Christopher Bankole Shogbon, Yue Zhou, Ralph Brandenberger
  • Patent number: 9730959
    Abstract: Provided is a biocomposite including cells, a genetic construction, and a scaffold and a method for repairing tissue and organs in mammalians with the biocomposite. The interaction of the components of the biocomposite provides a complex effect on reparative regeneration processes. Also provided is a method for administration of gene-cellular therapeutic constructions to a recipient which can be used in medicine and veterinary to provide reparative processes. After administering the biocomposite to a recipient, the scaffold structure releases the nucleic acids which enter into the cells of a recipient bed and cells of the transplanted product. The nucleic acids are expressed, which results in the increased concentration of a target product responsible for reparative processes.
    Type: Grant
    Filed: December 24, 2012
    Date of Patent: August 15, 2017
    Assignee: “NEXTGEN” COMPANY LIMITED
    Inventors: Artur Aleksandrovich Isaev, Sergej L'vovich Kiselev, Roman Vadimovich Deev, Il'ya Yadigerovich Bozo, Elena Sergeevna Filonenko
  • Patent number: 9617507
    Abstract: An apparatus that provides synchronized chronic electrical stimulation and chronic stretch to a cell culture or tissue. The apparatus includes a programmable controller which synchronizes stretch and electrical stimulation applied to a deformable culture container, such as a silicone rubber culture dish. The apparatus is used to treat cells and retard de-differentiation or promote differentiation and maturation in some applications.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: April 11, 2017
    Assignee: IONOPTIX LLC
    Inventors: Peter G. W. Lee, Douglas L. Tillotson, Richard T. Udale, Katherine L. Barber
  • Patent number: 9499846
    Abstract: A method for converting flue gas into useable bio-oil based compounds using algae includes mixing flue gas with water, infusing the mixture with a lipid trigger and nutrients, distributing the mixture into processing cells, exposing the mixture to light, promoting the algae to the top of the processing cells for harvest, skimming the algae from the surface of the water, and extracting bio-oil from the algae.
    Type: Grant
    Filed: October 30, 2014
    Date of Patent: November 22, 2016
    Inventor: Mark Randall
  • Patent number: 9493735
    Abstract: A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.
    Type: Grant
    Filed: April 13, 2006
    Date of Patent: November 15, 2016
    Assignee: Wake Forest University Health Sciences
    Inventors: James Yoo, Joel Stitzel, Anthony Atala, George Christ
  • Patent number: 9476834
    Abstract: A method for preparing a linearly extended supramolecular fiber or a plurality of linearly aligned supramolecular fibers, which comprises the step of allowing supramolecular monomers to be self-assembled in a microfluidic channel.
    Type: Grant
    Filed: September 17, 2010
    Date of Patent: October 25, 2016
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Shoji Takeuchi, Hiroaki Onoe, Daisuke Kiriya, Itaru Hamachi, Masato Ikeda
  • Patent number: 9421304
    Abstract: A double-structured tissue implant (DSTI) and a method for preparation and use thereof for implantation into tissue defects. The double-structured tissue implant for differentiation, growth and transformation of cells, stem cells, mesenchymal stem cells and bone marrow stem cells. DSTI comprising a primary scaffold and a secondary scaffold consisting of a soluble collagen solution in combination with a non-ionic surfactant generated and positioned within the primary scaffold.
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: August 23, 2016
    Assignee: Histogenics Corporation
    Inventors: Sonya Shortkroff, Joseph Khoury, Laurence J. B. Tarrant, Hans P. I. Claesson, Robert Lane Smith
  • Patent number: 9408797
    Abstract: The present invention provides highly injectable, long-lasting hyaluronic acid-based hydrogel dermal filler compositions which are particularly advantageous for correction of fine lines in the face.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: August 9, 2016
    Assignee: Allergan, Inc.
    Inventors: Gabriel N. Njikang, Futian Liu, Xiaojie Yu, Nicholas J. Manesis, Sumit Paliwal
  • Patent number: 9393263
    Abstract: Provided are injectable, hyaluronic acid-based dermal filler compositions including conjugated vitamin C derivatives. The compositions provide for extended release of active vitamin C into tissue, promoting collagenesis and other benefits to skin.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: July 19, 2016
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Jessamine P. Winer-Jones, Nicholas J. Manesis, Hengli Liu, Gabriel Njikang
  • Patent number: 9388379
    Abstract: A dynamically alterable cell support may be altered at a large scale to induce mechanical removal of adherent cells in culture without the use of a removal solution. For example, adherent cells may be cultured on an elastic support with one or more textured surface regions and removed by expansion/contraction of the support. Mechanical removal of adherent cells may reduce or minimize damage to cell surface markers, cellular morphology, and/or cellular physiology associated with the detachment and resuspension of cultured adherent cells.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: July 12, 2016
    Assignee: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventor: Ezekiel Kruglick
  • Patent number: 9375513
    Abstract: Methods of making tissue fillers are provided. In certain embodiments, the tissue is flake-like and has regenerative properties.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: June 28, 2016
    Assignee: LifeCell Corporation
    Inventors: Wenquan Sun, Melissa Richter Bowley, Raghav Goel
  • Patent number: 9353345
    Abstract: A securing apparatus includes a housing, a cam member, and a movable device that can be moved in a first direction to engage a first surface of the cam member to cause the cam member to move from a first position to a second position. When the cam member is in the first position, first and second movable members can be moved relative to the housing. When the cam member is in the second position, second and third surfaces of the cam member press respectively against the first and second movable members to trap the first and second movable members respectively between the second and third surfaces of the cam member and the housing such that the first and second movable members are fixed relative to the housing.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: May 31, 2016
    Assignee: TA INSTRUMENTS-WATERS L.L.C.
    Inventors: Guangtian Zhang, Troy D. Nickel, Eugene Joseph Rausch, Aaron M. Owens
  • Patent number: 9283283
    Abstract: The present invention relates to hydrogels based on hyaluronic acid based derivatives, that are more resistant than hyaluronic acid alone towards chemical and enzymatic degradation and are endowed with a mix of chemical and mechanical properties such that they are of optimal use in several surgeries, for instance for injection in bone fractures or cavities and for the production of coatings of prostheses in orthopedic surgery, as fillers in cosmetic and maxillo-facial surgery, as anti-adhesion barrier in the prevention of postoperative adhesions in abdominal and abdominal/pelvic surgery, and in general surgery. These hydrogels can be loaded with several different kinds of compounds having pharmacological and biological activity, that are then released from the hydrogel in the site of intervention.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: March 15, 2016
    Assignees: MERO S.R.L., NOVAGENIT S.R.L.
    Inventors: Gaetano Giammona, Giovanna Pitarresi, Fabio Palumbo, Carlo Luca Romano′, Enzo Meani, Edgardo Cremascoli
  • Patent number: 9243229
    Abstract: Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.
    Type: Grant
    Filed: September 17, 2013
    Date of Patent: January 26, 2016
    Assignee: Asterias Biotherapeutics, Inc.
    Inventors: Christopher Bankole Shogbon, Yue Zhou, Ralph Brandenberger
  • Patent number: 9233124
    Abstract: The invention relates to a biomaterial containing calcium phosphate, in particular hydroxyapatite or a material containing hydroxyapatite, such as biphasic calcium phosphates and calcium phosphate cements, and to the use thereof for the production of an implant or for fitting a prosthesis for the purpose of bone tissue regeneration.
    Type: Grant
    Filed: June 22, 2009
    Date of Patent: January 12, 2016
    Assignees: Centre National de la Recherche Scientifique, Centre Hospitalier Universitaire De Nice
    Inventors: Thierry Balaguer, Nathalie Rochet, Georges Carle
  • Patent number: 9192695
    Abstract: There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate and the MSCs are allowed to adhere. The substrate is rinsed to remove unwanted cells. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate made according to the described method. Other embodiments are also disclosed.
    Type: Grant
    Filed: November 4, 2009
    Date of Patent: November 24, 2015
    Assignee: AlloSource
    Inventor: Yaling Shi
  • Patent number: 9163206
    Abstract: There is provided a culture apparatus including: a first pipe having a discharge channel formed at one end thereof; a second pipe having a suction channel formed at one end thereof; and a connection channel connecting the first and second pipes.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: October 20, 2015
    Assignee: Samsung Electro-Mechanics Co., Ltd.
    Inventors: Dong Woo Lee, Sang Jin Kim
  • Patent number: 9149422
    Abstract: Provided are injectable, hyaluronic acid-based hydrogel compositions including conjugated vitamins.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: October 6, 2015
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Nicholas J. Manesis
  • Publication number: 20150140654
    Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
    Type: Application
    Filed: November 14, 2014
    Publication date: May 21, 2015
    Applicant: TERUMO BCT, INC.
    Inventors: Brian J. NANKERVIS, Mark E. JONES
  • Patent number: 9034647
    Abstract: Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition may also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function.
    Type: Grant
    Filed: May 5, 2014
    Date of Patent: May 19, 2015
    Assignee: CORMATRIX CARDIOVASCULAR, INC.
    Inventor: Robert G Matheny