Solid Support And Method Of Culturing Cells On Said Solid Support Patents (Class 435/395)
  • Patent number: 10071187
    Abstract: Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: September 11, 2018
    Assignees: Cook Biotech Incorporated, Muffin Incorporated
    Inventors: Michael C. Hiles, Chad E. Johnson, Neal E. Fearnot, Thomas Payne, Ronald Jankowski
  • Patent number: 10004830
    Abstract: The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells (Human mesenchymal stem cells, Human fibroblast, Keratinocytes, fibroblast differentiated form mesenchymal stem cells in vitro and keratinocytes differentiated form mesenchymal stem cells in vitro) in vitro for continued periods of time. In the present invention, mesenchymal stem cells (MSCs) derived from bone marrow or umbilical cord are inoculated and grown on a pre-established scaffold or matrix made up of chitosan and gelatin. The developed tissue constructs contain cells, growth factors, cytokines and other regulatory factors secreted by cells. The developed bioengineered tissue constructs provide the support, growth factors and essential cytokines for wound healing. Developed tissue construct useful in the treatment of burns wound, diabetic foot ulcer wound and type of chronic skin wounds. It is helpful in healing and regeneration of skin tissue.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: June 26, 2018
    Assignee: DATT MEDIPRODUCTS LIMITED and DATT LIFE SCIENCE PVT. LTD.
    Inventors: Rajan Datt, Siddharth Pandey, Pallavi Shrivastava, Supriya Srivastava
  • Patent number: 9962464
    Abstract: Provided are injectable, hyaluronic acid-based dermal filler compositions including conjugated vitamin C derivatives. The compositions provide for extended release of active vitamin C into tissue, promoting collagenesis and other benefits to skin.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: May 8, 2018
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Jessamine P. Winer-Jones, Nicholas J. Manesis, Hengli Liu, Gabriel Njikang, Athene Wan Chie Chan
  • Patent number: 9913700
    Abstract: The present invention relates to a biomimetic matrix for providing structural support and scaffolding that allows for regeneration of dentin, pulp, and periodontal tissues. A method of making the biomimetic matrix provides the ability to select both a size of a pore or tubule formed in the biomimetic matrix and a density of pores or tubules disposed throughout the biomimetic matrix. The present invention discloses an approach of successful tubular dentin regeneration both in vitro and in vivo using the biomimetic matrix.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: March 13, 2018
    Assignee: The Texas A&M University System
    Inventors: Xiaohua Liu, Chi Ma, Tiejun Qu
  • Patent number: 9873861
    Abstract: The invention relates to implantable collagen devices made by seeding at least one elongate collagen construct, e.g., comprising at least one elongate synthetic collagen fiber with a plurality of cells and applying a strain and/or stress to the at least one elongate collagen fiber to induce the cells to differentiate into target phenotypes, e.g., tendon or ligament phenotype cells (and/or fibroblasts), typically with an extracellular matrix of collagen to organize into a tissue on the at least one collagen fiber.
    Type: Grant
    Filed: February 10, 2015
    Date of Patent: January 23, 2018
    Assignee: MiMedx Group, Inc.
    Inventor: Thomas J. Koob
  • Patent number: 9814802
    Abstract: A method of differentiating cells into CK19-positive cells capable of producing hair follicle-like and hair structure-like can include: providing a tissue scaffold; seeding cells into the scaffold, the cells being capable of differentiation; incubating the scaffold having the cells in a cell growth media; and incubating the scaffold having the cells in an osteogenic differentiation medium sufficient for CK19-positive cells to be generated in the scaffold. The tissue scaffold can be a decellularized Whartons' jelly matrix. The cell growth media excludes osteogenic differentiation components: dexamethasone, ?-glycerophosphate, 1?,25-hydroxyvitamin D3, and ascorbic acid 2-phosphate. The osteogenic differentiation medium includes the osteogenic differentiation components. The cells can be mesenchymal cells, such as WJMSCs.
    Type: Grant
    Filed: April 30, 2013
    Date of Patent: November 14, 2017
    Assignees: The University of Kansas, The Children's Mercy Hospital
    Inventors: Omar Aljitawi, Richard Hopkins, Michael Detamore, Rama Garimella
  • Patent number: 9777326
    Abstract: A method of sequencing nucleic acids, which can include steps of (a) providing a substrate comprising a surface having a repeating pattern of features, wherein the features are spatially separated from each other on the surface of the substrate; (b) contacting the repeating pattern of features with a solution of different nucleic acids to seed a subset of the features that contact the solution, wherein each feature in the subset is seeded with a single nucleic acid from the solution, and a plurality of the features that contact the solution are not seeded with a nucleic acid from the solution; (c) amplifying the nucleic acids to form a nucleic acid colony at each of the features in the seeded subset; (d) repeating steps (b) and (c) to increase the number of features that are seeded with a nucleic acid, thereby making an array of different nucleic acid colonies; and (e) detecting sequencing reactions at the different nucleic acid colonies on the surface.
    Type: Grant
    Filed: August 15, 2016
    Date of Patent: October 3, 2017
    Assignee: ILLUMINA, INC.
    Inventors: Mostafa Ronaghi, Helmy A. Eltoukhy
  • Patent number: 9752112
    Abstract: The pressure and circulation culture apparatus includes a holder (culture chamber part), a pressure transmission part and a culture solution circulation line (culture circuit). The holder reserves culture solution and holds a cultured object. The pressure transmission part communicates with the holder and transmits an external pressure to the culture solution. The culture solution circulation line is connected to the holder via the pressure transmission part, and circulates the culture solution through the holder via the pressure transmission part. Such a structure is included, and an external pressure is made to operate on the culture solution via the pressure transmission part to allow pressure on the cultured object in the holder and circulation of the culture solution in the holder.
    Type: Grant
    Filed: February 23, 2012
    Date of Patent: September 5, 2017
    Assignee: PURPOSE CO., LTD.
    Inventors: Setuo Watanabe, Toshimi Murata, Mituharu Satou, Ibuki Kinouchi, Hidetada Takai
  • Patent number: 9752116
    Abstract: A composition comprising a plurality of cell aggregates for use in the production of engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension to form a pellet, extruding the pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing processes parameters for use in producing an engineered tissue having a desired three-dimensional structure.
    Type: Grant
    Filed: August 12, 2016
    Date of Patent: September 5, 2017
    Assignees: The Curators of the University of Missouri, MUSC Foundation for Research Development
    Inventors: Gabor Forgacs, Karoly Jakab, Adrian Neagu, Vladimir Mironov
  • Patent number: 9744041
    Abstract: A three component system for repairing critically sized bone defects having a first shape memory polymer (SMP) component formed as a scaffold that fills the defects, a second SMP component formed as a restricting sleeve that stabilizes and supports osseointegration and osteoconduction, and a third SMP component formed as a two-dimensional cell culture substrate for engineering periosteal grafts.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: August 29, 2017
    Assignee: Syracuse University
    Inventors: James Henderson, Patrick T. Mather
  • Patent number: 9745550
    Abstract: Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.
    Type: Grant
    Filed: January 25, 2016
    Date of Patent: August 29, 2017
    Assignee: Asterias Biotherapeutics, Inc.
    Inventors: Christopher Bankole Shogbon, Yue Zhou, Ralph Brandenberger
  • Patent number: 9730959
    Abstract: Provided is a biocomposite including cells, a genetic construction, and a scaffold and a method for repairing tissue and organs in mammalians with the biocomposite. The interaction of the components of the biocomposite provides a complex effect on reparative regeneration processes. Also provided is a method for administration of gene-cellular therapeutic constructions to a recipient which can be used in medicine and veterinary to provide reparative processes. After administering the biocomposite to a recipient, the scaffold structure releases the nucleic acids which enter into the cells of a recipient bed and cells of the transplanted product. The nucleic acids are expressed, which results in the increased concentration of a target product responsible for reparative processes.
    Type: Grant
    Filed: December 24, 2012
    Date of Patent: August 15, 2017
    Assignee: “NEXTGEN” COMPANY LIMITED
    Inventors: Artur Aleksandrovich Isaev, Sergej L'vovich Kiselev, Roman Vadimovich Deev, Il'ya Yadigerovich Bozo, Elena Sergeevna Filonenko
  • Patent number: 9617507
    Abstract: An apparatus that provides synchronized chronic electrical stimulation and chronic stretch to a cell culture or tissue. The apparatus includes a programmable controller which synchronizes stretch and electrical stimulation applied to a deformable culture container, such as a silicone rubber culture dish. The apparatus is used to treat cells and retard de-differentiation or promote differentiation and maturation in some applications.
    Type: Grant
    Filed: October 1, 2013
    Date of Patent: April 11, 2017
    Assignee: IONOPTIX LLC
    Inventors: Peter G. W. Lee, Douglas L. Tillotson, Richard T. Udale, Katherine L. Barber
  • Patent number: 9499846
    Abstract: A method for converting flue gas into useable bio-oil based compounds using algae includes mixing flue gas with water, infusing the mixture with a lipid trigger and nutrients, distributing the mixture into processing cells, exposing the mixture to light, promoting the algae to the top of the processing cells for harvest, skimming the algae from the surface of the water, and extracting bio-oil from the algae.
    Type: Grant
    Filed: October 30, 2014
    Date of Patent: November 22, 2016
    Inventor: Mark Randall
  • Patent number: 9493735
    Abstract: A method of producing organized skeletal muscle tissue from precursor muscle cells in vitro comprises: (a) providing precursor muscle cells on a support in a tissue media; then (b) cyclically stretching and relaxing the support at least twice along a first axis during a first time period; and then (c) optionally but preferably maintaining the support in a substantially static position during a second time period; and then (d) repeating steps (b) and (c) for a number of times sufficient to enhance the functionality of the tissue formed on the support and/or produce organized skeletal muscle tissue on the solid support from the precursor muscle cells.
    Type: Grant
    Filed: April 13, 2006
    Date of Patent: November 15, 2016
    Assignee: Wake Forest University Health Sciences
    Inventors: James Yoo, Joel Stitzel, Anthony Atala, George Christ
  • Patent number: 9476834
    Abstract: A method for preparing a linearly extended supramolecular fiber or a plurality of linearly aligned supramolecular fibers, which comprises the step of allowing supramolecular monomers to be self-assembled in a microfluidic channel.
    Type: Grant
    Filed: September 17, 2010
    Date of Patent: October 25, 2016
    Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
    Inventors: Shoji Takeuchi, Hiroaki Onoe, Daisuke Kiriya, Itaru Hamachi, Masato Ikeda
  • Patent number: 9421304
    Abstract: A double-structured tissue implant (DSTI) and a method for preparation and use thereof for implantation into tissue defects. The double-structured tissue implant for differentiation, growth and transformation of cells, stem cells, mesenchymal stem cells and bone marrow stem cells. DSTI comprising a primary scaffold and a secondary scaffold consisting of a soluble collagen solution in combination with a non-ionic surfactant generated and positioned within the primary scaffold.
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: August 23, 2016
    Assignee: Histogenics Corporation
    Inventors: Sonya Shortkroff, Joseph Khoury, Laurence J. B. Tarrant, Hans P. I. Claesson, Robert Lane Smith
  • Patent number: 9408797
    Abstract: The present invention provides highly injectable, long-lasting hyaluronic acid-based hydrogel dermal filler compositions which are particularly advantageous for correction of fine lines in the face.
    Type: Grant
    Filed: September 13, 2012
    Date of Patent: August 9, 2016
    Assignee: Allergan, Inc.
    Inventors: Gabriel N. Njikang, Futian Liu, Xiaojie Yu, Nicholas J. Manesis, Sumit Paliwal
  • Patent number: 9393263
    Abstract: Provided are injectable, hyaluronic acid-based dermal filler compositions including conjugated vitamin C derivatives. The compositions provide for extended release of active vitamin C into tissue, promoting collagenesis and other benefits to skin.
    Type: Grant
    Filed: August 23, 2012
    Date of Patent: July 19, 2016
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Jessamine P. Winer-Jones, Nicholas J. Manesis, Hengli Liu, Gabriel Njikang
  • Patent number: 9388379
    Abstract: A dynamically alterable cell support may be altered at a large scale to induce mechanical removal of adherent cells in culture without the use of a removal solution. For example, adherent cells may be cultured on an elastic support with one or more textured surface regions and removed by expansion/contraction of the support. Mechanical removal of adherent cells may reduce or minimize damage to cell surface markers, cellular morphology, and/or cellular physiology associated with the detachment and resuspension of cultured adherent cells.
    Type: Grant
    Filed: February 25, 2011
    Date of Patent: July 12, 2016
    Assignee: EMPIRE TECHNOLOGY DEVELOPMENT LLC
    Inventor: Ezekiel Kruglick
  • Patent number: 9375513
    Abstract: Methods of making tissue fillers are provided. In certain embodiments, the tissue is flake-like and has regenerative properties.
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: June 28, 2016
    Assignee: LifeCell Corporation
    Inventors: Wenquan Sun, Melissa Richter Bowley, Raghav Goel
  • Patent number: 9353345
    Abstract: A securing apparatus includes a housing, a cam member, and a movable device that can be moved in a first direction to engage a first surface of the cam member to cause the cam member to move from a first position to a second position. When the cam member is in the first position, first and second movable members can be moved relative to the housing. When the cam member is in the second position, second and third surfaces of the cam member press respectively against the first and second movable members to trap the first and second movable members respectively between the second and third surfaces of the cam member and the housing such that the first and second movable members are fixed relative to the housing.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: May 31, 2016
    Assignee: TA INSTRUMENTS-WATERS L.L.C.
    Inventors: Guangtian Zhang, Troy D. Nickel, Eugene Joseph Rausch, Aaron M. Owens
  • Patent number: 9283283
    Abstract: The present invention relates to hydrogels based on hyaluronic acid based derivatives, that are more resistant than hyaluronic acid alone towards chemical and enzymatic degradation and are endowed with a mix of chemical and mechanical properties such that they are of optimal use in several surgeries, for instance for injection in bone fractures or cavities and for the production of coatings of prostheses in orthopedic surgery, as fillers in cosmetic and maxillo-facial surgery, as anti-adhesion barrier in the prevention of postoperative adhesions in abdominal and abdominal/pelvic surgery, and in general surgery. These hydrogels can be loaded with several different kinds of compounds having pharmacological and biological activity, that are then released from the hydrogel in the site of intervention.
    Type: Grant
    Filed: July 29, 2011
    Date of Patent: March 15, 2016
    Assignees: MERO S.R.L., NOVAGENIT S.R.L.
    Inventors: Gaetano Giammona, Giovanna Pitarresi, Fabio Palumbo, Carlo Luca Romano′, Enzo Meani, Edgardo Cremascoli
  • Patent number: 9243229
    Abstract: Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.
    Type: Grant
    Filed: September 17, 2013
    Date of Patent: January 26, 2016
    Assignee: Asterias Biotherapeutics, Inc.
    Inventors: Christopher Bankole Shogbon, Yue Zhou, Ralph Brandenberger
  • Patent number: 9233124
    Abstract: The invention relates to a biomaterial containing calcium phosphate, in particular hydroxyapatite or a material containing hydroxyapatite, such as biphasic calcium phosphates and calcium phosphate cements, and to the use thereof for the production of an implant or for fitting a prosthesis for the purpose of bone tissue regeneration.
    Type: Grant
    Filed: June 22, 2009
    Date of Patent: January 12, 2016
    Assignees: Centre National de la Recherche Scientifique, Centre Hospitalier Universitaire De Nice
    Inventors: Thierry Balaguer, Nathalie Rochet, Georges Carle
  • Patent number: 9192695
    Abstract: There is disclosed a method of combining mesenchymal stem cells (MSCs) with a bone substrate. In an embodiment, the method includes obtaining tissue having MSCs together with unwanted cells. The tissue is digested to form a cell suspension having MSCs and unwanted cells. The cell suspension is added to the substrate and the MSCs are allowed to adhere. The substrate is rinsed to remove unwanted cells. In an embodiment, there is disclosed an allograft product including a combination of MSCs with a bone substrate made according to the described method. Other embodiments are also disclosed.
    Type: Grant
    Filed: November 4, 2009
    Date of Patent: November 24, 2015
    Assignee: AlloSource
    Inventor: Yaling Shi
  • Patent number: 9163206
    Abstract: There is provided a culture apparatus including: a first pipe having a discharge channel formed at one end thereof; a second pipe having a suction channel formed at one end thereof; and a connection channel connecting the first and second pipes.
    Type: Grant
    Filed: February 2, 2012
    Date of Patent: October 20, 2015
    Assignee: Samsung Electro-Mechanics Co., Ltd.
    Inventors: Dong Woo Lee, Sang Jin Kim
  • Patent number: 9149422
    Abstract: Provided are injectable, hyaluronic acid-based hydrogel compositions including conjugated vitamins.
    Type: Grant
    Filed: June 1, 2012
    Date of Patent: October 6, 2015
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Nicholas J. Manesis
  • Publication number: 20150140654
    Abstract: Described are embodiments for expanding cells in a bioreactor. In one embodiment, methods are provided that distribute cells throughout the bioreactor and attach cells to specific portions of a bioreactor to improve the expansion of the cells in the bioreactor. Embodiments may be implemented on a cell expansion system configured to load, distribute, attach and expand cells.
    Type: Application
    Filed: November 14, 2014
    Publication date: May 21, 2015
    Applicant: TERUMO BCT, INC.
    Inventors: Brian J. NANKERVIS, Mark E. JONES
  • Patent number: 9034571
    Abstract: Three-dimensional (3D), prevascularized, engineered tissue constructs, 3D prevascularized engineered tissue models of cancer, and bioreactors and bioreactor arrays including the tissue constructs are disclosed. Methods of making the tissue constructs, methods of using the tissue constructs, methods of drug discovery using the tissue constructs and/or cancer models, and the like are also disclosed.
    Type: Grant
    Filed: March 6, 2013
    Date of Patent: May 19, 2015
    Assignees: THE UAB RESEARCH FOUNDATION, SOUTHERN RESEARCH INSTITUTE
    Inventors: Joel L. Berry, Timothy M. Wick, Joanne Murphy-Ullrich, Andrew D. Penman, Andrew W. Cain, Andra Rixse Frost
  • Patent number: 9034647
    Abstract: Compositions of the invention for regenerating defective or absent myocardium comprise an emulsified or injectable extracellular matrix composition. The composition may also include an extracellular matrix scaffold component of any formulation, and further include added cells, proteins, or other components to optimize the regenerative process and restore cardiac function.
    Type: Grant
    Filed: May 5, 2014
    Date of Patent: May 19, 2015
    Assignee: CORMATRIX CARDIOVASCULAR, INC.
    Inventor: Robert G Matheny
  • Patent number: 9034648
    Abstract: An object of the present invention is to provide an artificial tissue construct that has means for transporting nutrients, oxygen, waste products, or the like and is viable in vivo. The present invention relates to a tissue construct formed in vitro, which comprises a vascular layer, a basal membrane layer, and a tissue-forming cell layer.
    Type: Grant
    Filed: June 21, 2013
    Date of Patent: May 19, 2015
    Assignee: DAI NIPPON PRINTING CO., LTD.
    Inventors: Ikuo Morita, Hideyuki Miyake, Hideshi Hattori, Hironori Kobayashi, Yusuke Uno
  • Patent number: 9029147
    Abstract: The invention provides methods for differentiating pluripotent stem cells such as ES cells with improved progenitor and differentiated cell yield using low oxygen conditions and optionally in the absence of exogenously added differentiation factors.
    Type: Grant
    Filed: June 13, 2008
    Date of Patent: May 12, 2015
    Assignee: Massachusetts Institute of Technology
    Inventors: Clark K. Colton, Daryl E. Powers, Jeffrey R. Millman
  • Patent number: 9029144
    Abstract: The present invention relates generally to methods for the isolation and propagation of cells. For example, embodiments of the present invention relate to isolation and propagation methods for the manufacture of a large number of cells for use, for example, in biotherapeutic devices, such as devices for renal replacement therapy for the treatment of acute renal failure (ARF), acute tubular necrosis (ATN), multi-organ failure (MOF), sepsis, cardiorenal syndrome (CRS) and end-stage renal disease (ESRD).
    Type: Grant
    Filed: June 16, 2009
    Date of Patent: May 12, 2015
    Assignees: Innovative Bio Therapies, Inc., CytoPherx, Inc.
    Inventors: Mark Szczypka, Evangelos Tziampazis, Angela J. Westover
  • Publication number: 20150125507
    Abstract: The present disclosure provides patterned biomaterials having organized cords and extracellular matrix embedded in a 3D scaffold. According, the present disclosure is provides compositions and applications for patterned biomaterials. Pre-patterning of these biomaterials can lead to enhanced integration of these materials into host organisms, providing a strategy for enhancing the viability of engineered tissues by promoting vascularization.
    Type: Application
    Filed: January 9, 2015
    Publication date: May 7, 2015
    Applicant: THE TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA
    Inventors: Christopher S. Chen, Jan D. Baranski, Ritika Chaturvedi, Michael T. Yang
  • Patent number: 9023341
    Abstract: The present invention relates to a novel injectable delivery system for stem cell therapy, which comprises a thermo-sensitive amphiphlic chitosan nanogel. Therefore, the invention provides a method for repairing a tissue damage of a subject using the amphiphlic chitosan nanogel served as a carrier for delivering the stem cells to the damaged tissue. This invention also provides a method for sustaining the growth of stem cells using the amphiphlic chitosan nanogel served as a niche or scaffold.
    Type: Grant
    Filed: February 19, 2013
    Date of Patent: May 5, 2015
    Assignee: Taipei Veterans General Hospital
    Inventors: Shih-Hwa Chiou, Dean-Mo Liu
  • Patent number: 9023642
    Abstract: A bioreactor and method that permits continuous and simultaneous short, moderate, or long term cell culturing of one or more cell types or tissue in a laminar flow configuration is disclosed, where the bioreactor supports at least two laminar flow zones, which are isolated by laminar flow without the need for physical barriers between the zones. The bioreactors of this invention are ideally suited for studying short, moderate and long term studies of cell cultures and the response of cell cultures to one or more stressors such as pharmaceuticals, hypoxia, pathogens, or any other stressor. The bioreactors of this invention are also ideally suited for short, moderate or long term cell culturing with periodic cell harvesting and/or medium processing for secreted cellular components.
    Type: Grant
    Filed: July 6, 2007
    Date of Patent: May 5, 2015
    Assignee: The University of Houston System
    Inventors: Stanley J. Kleis, Sandra K. Geffert, Steve R. Gonda
  • Publication number: 20150118308
    Abstract: The present invention provides a process or preparing an extracellular matrix composition which comprises: (a) mixing an aqueous solution of fibrinogen with a coagulating agent and a bulking agent and a foaming agent; (b) causing the mixture to foam and coagulate; (c) incubating the mixture obtained in step (b) with a cross-linking agent; and (d) washing the cross-linked composition obtained in step (c) to remove the cross-linking agent. Wherein the foaming agent consists of or comprises one or more surfactant agent(s) from the class of sugar-surfactants. The invention also relates to the formulation mixture as such, and to the products of the process.
    Type: Application
    Filed: May 3, 2013
    Publication date: April 30, 2015
    Inventor: Julian F. Dye
  • Publication number: 20150110753
    Abstract: The present invention provides biomatrix scaffolds, a tissue extract enriched for extracellular matrix components and bound growth factors, cytokines and hormones, and methods of making and using same.
    Type: Application
    Filed: August 8, 2014
    Publication date: April 23, 2015
    Applicant: THE UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
    Inventors: Yunfang WANG, Lola Cynthia McAdams REID, Mitsuo YAMAUCHI, Cai-Bin CUI, Andrew Zhuang WANG, Michael Edward WERNER
  • Patent number: 9011895
    Abstract: A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue to chemical and mechanical processing. In addition to skin tissue, another source of EBM is a blood vessel. EBM may be used for hernia repair, colon, rectal, vaginal and or urethral prolapse treatment; pelvic floor reconstruction; muscle flap reinforcement; lung tissue support; rotator cuff repair or replacement; periosteum replacement; dura repair; pericardial membrane repair; soft tissue augmentation; intervertebral disk repair; and periodontal repair. EBM may also be used as a urethral sling, laminectomy barrier or spinal fusion device.
    Type: Grant
    Filed: November 18, 2008
    Date of Patent: April 21, 2015
    Assignee: TEI Biosciences, Inc.
    Inventors: Jianwu Dai, Eugene Bell, Vladimir Russakovsky
  • Publication number: 20150104872
    Abstract: A cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof. The matrix suitable for preparation of cellular or acellular implants for growth and de novo formation of an articular hyaline-like cartilage. A gel-matrix composite system comprising collagen-based matrix having a narrowly defined porosity capable of inducing hyaline-like cartilage production from chondrocytes in vivo and in vitro.
    Type: Application
    Filed: December 19, 2014
    Publication date: April 16, 2015
    Inventors: R. Lane Smith, Laurence J.B. Tarrant, Akihiko Kusanagi, Hans P.I. Claesson
  • Patent number: 9005970
    Abstract: [PROBLEMS] Relating to a bioassay method with the use of cultured cells and cell culture for a therapeutic purpose in the case of determining the efficacy of a drug or the like or examining the toxicity thereof, it is intended to provide a method of culturing cells under regulation in the extension direction and a cell culture plate. [MEANS FOR SOLVING PROBLEMS] A cell culture plate having a plural number of side walls (1) and a plural number of space structures (3) for providing cultured cells which are formed by the side walls (1), wherein the side walls (1) are provided with openings (2) so that the space structures (3) are linked together. By using this cell culture plate, a method of culturing cells under regulation in the extension direction can be provided.
    Type: Grant
    Filed: January 11, 2006
    Date of Patent: April 14, 2015
    Assignees: Kuraray Co., Ltd., Ryosuke Nishio
    Inventors: Ryosuke Nishio, Taiji Nishi, Motohiro Fukuda, Yasuzo Kirita, Seiichi Kanai, Takenori Kitani
  • Patent number: 9005604
    Abstract: A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct.
    Type: Grant
    Filed: December 15, 2010
    Date of Patent: April 14, 2015
    Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
    Inventors: Lisa A. Scott-Carnell, Emilie J. Siochi, Nancy M. Holloway, Kam W. Leong, Karina Kulangara
  • Patent number: 9005972
    Abstract: Provided herein is an apparatus for printing cells which includes an electrospinning device and an inkjet printing device operatively associated therewith. Methods of making a biodegradable scaffold having cells seeded therein are also provided. Methods of forming microparticles containing one or more cells encapsulated by a substrate are also provided, as are methods of forming an array of said microparticles.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: April 14, 2015
    Assignee: Wake Forest University Health Sciences
    Inventors: Tao Xu, James J. Yoo, Anthony Atala, Dennis Dice
  • Patent number: 8999711
    Abstract: Bone cages are disclosed including devices for biocompatible implantation. The structures of bone are useful for providing living cells and tissues as well as biologically active molecules to subjects.
    Type: Grant
    Filed: September 12, 2007
    Date of Patent: April 7, 2015
    Assignee: The Invention Science Fund I, LLC
    Inventors: Ed Harlow, Edward K. Y. Jung, Robert Langer, Eric C. Leuthardt, Lowell L. Wood, Jr.
  • Publication number: 20150094808
    Abstract: A synthetic scaffold for replacing at least a portion of an airway includes an airway mold, one or more structural ribs on the airway mold, and a non-structural wall. Each of the one or more structural ribs is formed from a first material and the non-structural wall is formed from a second material. The non-structural wall coats the airway mold and forms a conduit that incorporates the one or more structural ribs.
    Type: Application
    Filed: June 11, 2014
    Publication date: April 2, 2015
    Applicant: HARVARD APPARATUS REGENERATIVE TECHNOLOGY, INC
    Inventor: Paolo Macchiarini
  • Publication number: 20150093428
    Abstract: A 3D in vitro bi-phasic cartilage-bone organoid includes a layer of an artificial cartilage tissue, and a layer of an artificial bone tissue comprising a structure-giving scaffold and a bone marrow structure. The layer of the artificial cartilage tissue contacts at least one surface of the layer of the artificial bone tissue.
    Type: Application
    Filed: February 28, 2013
    Publication date: April 2, 2015
    Applicant: TISSUSE GMBH
    Inventors: Mark Rosowski, Shirin Fatehi-Varkani, Roland Lauster, Uwe Marx
  • Patent number: 8993324
    Abstract: In a process for the cultivation of living cells, in which the cells are cultivated on a support structure (14), the support structure (14) comprises cellulose. A process for the production of a support structure (14) of cellulose for the cultivation of living cells comprises the steps: preparation of a hollow mould; cultivation of cellulose-forming organisms in an interior space formed by the hollow mould, in order to allow the support structure (14) to grow in the interior space; demoulding of the hollow mould. In the step of demoulding the hollow mould, at least part (2, 3, 4) of the hollow mould is irreversibly deformed.
    Type: Grant
    Filed: February 12, 2008
    Date of Patent: March 31, 2015
    Assignee: Bioregeneration GmbH
    Inventor: Günter Bertholdt
  • Patent number: 8986678
    Abstract: The present invention relates to methods of in vitro preparation of a parental cell bank (PCB) from foetal tissue consisting of foetal epiphyseal tissue, foetal Achilles tendon tissue and foetal skin tissue, using a rapid mechanical primary cell culture selection of cell type to be used in methods for wound and tissue repair.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: March 24, 2015
    Assignee: Centre Hospitalier Universitaire Vaudois
    Inventor: Lee Ann Laurent-Applegate
  • Patent number: 8980628
    Abstract: This invention relates to methods of producing hair folliclesin vitro, compositions for producing hair follicles in vitro, in vitro produced hair follicles, methods of providing an in vitro produced hair shaft at an interfollicular or intrafollicular site, methods of treating hair loss by providing an in vitro produced hair shaft at an interfollicular or intrafollicular site and assays for studying the effect of test agents on hair biology. The invention also provides the similar methods and products which are, or use, immature follicles (“defined herein as proto-hairs”). The invention provides a method for in vitro production of a hair follicle or a proto-hair comprising co-culturing dermal papilla cells with keratinocytes, and optionally with melanocytes.
    Type: Grant
    Filed: March 15, 2007
    Date of Patent: March 17, 2015
    Assignee: Aderans Research Institute, Inc.
    Inventors: Jizeng Qiao, Jeffrey Keeler Teumer, Erica Jean Philips