Solid Support And Method Of Culturing Cells On Said Solid Support Patents (Class 435/395)
  • Patent number: 11786633
    Abstract: A bioink composition and a 3D bioprinted scar tissue model with the bioink composition closely replicates the physiological and architectural characteristics of naturally occurring scar tissue. The 3D bioprinted scar tissue can be used to test scar resolution treatments among others. Also provided is a method of fabricating the 3D bioprinted scar tissue along with an apparatus for bioprinting the 3D bioprinted scar tissue.
    Type: Grant
    Filed: November 30, 2018
    Date of Patent: October 17, 2023
    Inventors: Sourabh Ghosh, Shikha Chawla
  • Patent number: 11746318
    Abstract: Methods and systems for measuring viability and function of islet cells or stem cell-derived beta cells in an implantable device featuring setting the temperature of the cells in the implantable device to a low temperature to reduce metabolic levels of the cells and reduce oxygen requirements of the cells, and measuring oxygen consumption rates. An oxygen sensor at the inlet of the implantable device and an oxygen sensor at the outlet of the implantable device are used to calculate oxygen consumption rates of the cells, which in turn are indicative of viability. The reduction in temperature can also be used for loading cells into the implantable devices to help reduce ischemic and/or physical injury. The present invention may be used with other cell types, e.g. hepatocytes, heart cells, muscle cells, etc.
    Type: Grant
    Filed: November 3, 2017
    Date of Patent: September 5, 2023
    Assignee: ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
    Inventor: Klearchos K. Papas
  • Patent number: 11746324
    Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGF? superfamily.
    Type: Grant
    Filed: December 10, 2021
    Date of Patent: September 5, 2023
    Assignee: Hadasit Medical Research Services and Development Ltd.
    Inventors: Benjamin Eithan Reubinoff, Orna Singer
  • Patent number: 11744923
    Abstract: The present invention relates to a monolithic multi-layered material having at least a first layer, from which anisotropic pores originate, and a second layer, in which the anisotropic pores continue. The present invention further relates to a monolithic medical material having at least a first layer, from which anisotropic pores originate, and a second layer, in which the anisotropic pores continue. The present invention further relates to a process for the production of a multi-layered material having anisotropic pores. It further relates to a multi-layered material which can be produced by the process according to the invention.
    Type: Grant
    Filed: January 21, 2020
    Date of Patent: September 5, 2023
    Assignee: TETEC Tissue Engineering Technologies AG
    Inventors: Kai Stuckensen, Uwe Gbureck, Juergen Groll
  • Patent number: 11744922
    Abstract: The present disclosure relates generally to tissue engineering. Disclosed herein are biomimetic sponges useful for tissue regeneration and methods for making biomimetic sponges.
    Type: Grant
    Filed: October 29, 2018
    Date of Patent: September 5, 2023
    Assignee: Saint Louis University
    Inventors: Koyal Garg, Gabriel Haas
  • Patent number: 11732290
    Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
    Type: Grant
    Filed: September 22, 2022
    Date of Patent: August 22, 2023
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11730860
    Abstract: Devices and methods for transplanting cells in a host body are described. The cell comprises a porous scaffold that allows ingrowth of vascular and connective tissues, a plug or plug system configured for placement within the porous scaffold, and a seal configured to enclose a proximal opening in the porous scaffold. The device may further comprise a cell delivery device for delivering cells into the porous scaffold. The method of cell transplantation comprises a two step process. The device is incubated in the host body to form a vascularized collagen matrix around a plug positioned within the porous scaffold. The plug is then retracted from the porous scaffold, and cells are delivered into the vascularized space created within the porous scaffold.
    Type: Grant
    Filed: January 7, 2019
    Date of Patent: August 22, 2023
    Assignee: Sernova Corporation
    Inventors: Craig Hasilo, Justin Leushner, Daniel Nicholas Haworth, Simon Shohet, Philip Michael Toleikis, Delfina Maria Mazzuca Siroen
  • Patent number: 11718824
    Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.
    Type: Grant
    Filed: May 27, 2021
    Date of Patent: August 8, 2023
    Assignee: CORNING INCORPORATED
    Inventors: Stephen Joseph Caracci, David Henry, Jessica Jo Kelley, Mark Alan Lewis, Yue Zhou
  • Patent number: 11702622
    Abstract: A system and method for studying cell injury mechanisms by applying biologically relevant mechanical impact to in vitro cell culture are disclosed. This approach is for maintaining consistent in vitro conditions during experiments, accommodating multiple cell populations, and monitoring each in real-time while achieving amplitude and time scale of input acceleration that mimic blunt injury cases. These multiplexed, environmental control capabilities enable characterizing the relationships between mechanical impact and cell injury in multivariate biological systems.
    Type: Grant
    Filed: August 21, 2020
    Date of Patent: July 18, 2023
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventors: Marc P. Raphael, Wonmo Kang
  • Patent number: 11666691
    Abstract: Bioartificial ultrafiltration devices comprising a scaffold comprising a population of cells enclosed in a matrix and disposed adjacent a plurality of channels are provided. The population of cells provides molecules such as therapeutic molecules to a subject in need thereof and is supported by the nutrients filtered in an ultrafiltrate from the blood of the subject. The plurality of channels in the scaffold facilitate the transportation of the ultrafiltrate and exchange of molecules between the ultrafiltrate and the population of cells.
    Type: Grant
    Filed: June 9, 2021
    Date of Patent: June 6, 2023
    Assignee: The Regents of the University of California
    Inventors: Shuvo Roy, Rishi Kant, Andrew Posselt, Shang Song, Willieford Moses, Charles Blaha
  • Patent number: 11667956
    Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
    Type: Grant
    Filed: September 22, 2022
    Date of Patent: June 6, 2023
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11654225
    Abstract: In one exemplary embodiment, a wearable extracorporeal life support device includes a catheter fluidly connected to a pump and first and second modular extracorporeal life support components. The device may also be configured to be attached to a garment. The pump and the first and second modular extracorporeal life support components may be fluidly connected in series. The pump and the first and second modular extracorporeal life support components may also be fluidly connected in parallel. The first modular extracorporeal life support component may be a lung membrane and the second modular extracorporeal life support component may be a dialysis membrane.
    Type: Grant
    Filed: June 27, 2019
    Date of Patent: May 23, 2023
    Assignee: The Geneva Foundation
    Inventors: Andriy I. Batchinsky, George T. Harea, Daniel S. Wendorff, Brendan M. Beely, Teryn R. Roberts
  • Patent number: 11655441
    Abstract: The present invention provides a cell culture base that avoids contact between a photoresponsive layer and cells. The cell culture base of the present invention includes: a support layer; a photoresponsive layer; and a cell culture layer, wherein the photoresponsive layer is laminated on the support layer, the cell culture layer is laminated on the photoresponsive layer, the photoresponsive layer contains a photoresponsive substance that causes at least one of heat and change in magnetism by light irradiation, and the cell culture layer is capable of culturing cells.
    Type: Grant
    Filed: April 20, 2020
    Date of Patent: May 23, 2023
    Assignee: KATAOKA CORPORATION
    Inventor: Junichi Matsumoto
  • Patent number: 11634675
    Abstract: The present invention relates to an insertable culture container and a kit for three-dimensional cell culture, and a three-dimensional cell co-culture method using the same, the insertable culture container for three-dimensional cell culture comprising: a cylindrical side wall having open upper and lower portions; at least one hook protruding outward from the upper side of the side wall; and at least one support protruding inward from the lower side of the side wall. The present invention is advantageous in that air required for a three-dimensional cell culture structure can be smoothly supplied since the cell is cultured at a position spaced apart from a bottom surface of the culture container, and an existing culture plate can be used without change due to the culture container configured as an insert type.
    Type: Grant
    Filed: December 7, 2018
    Date of Patent: April 25, 2023
    Assignees: KOREA INSTITUTE OF INDUSTRIAL TECHNOLOGY, CEFO CO., LTD.
    Inventors: Dongmok Lee, Hyunsook Park, Sunray Lee
  • Patent number: 11635430
    Abstract: An expanded polytetrafluoroethylene substrate comprising a microporous microstructure, an interlayer over at least a portion of the microstructure, the interlayer containing a reactive functionality, and a functional layer attached to the interlayer, the interlayer comprising a sol-gel or a polyvinylalcohol. The functional layer of the substrate having functional sites with a density of at least 50 nanomoles/cm2.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: April 25, 2023
    Assignee: W. L. Gore & Associates, Inc.
    Inventors: Anit Dutta, Heidi Flannery, William P. Mortimer, Jr.
  • Patent number: 11628602
    Abstract: Filter made entirely with natural and biodegradable materials, for the protection of the respiratory tract of patients in the medical-surgical field and process for making it. The filter has a porosity or 80-98% and pore diameters of 100-350 micrometers and the pores have a shape of channels open at the ends, which are parallel to each other. The filter is obtained by preparing an aqueous solution of chitosan and an aqueous solution of gelatin, mixing them, pouring it into a container, keeping the container closed until obtaining a hydrogel and freeze-drying. A cross-linking step takes place by (a) adding a chemical cross-linker to the mixture of the chitosan and gelatine solutions and cross-linking before freeze-drying, or (b) subjecting the freeze-drying product to a thermal dehydration treatment.
    Type: Grant
    Filed: February 27, 2018
    Date of Patent: April 18, 2023
    Assignee: CONSIGLIO NAZIONALE DELLE RICERCHE
    Inventors: Monica Sandri, Anna Tampieri, Simone Sprio
  • Patent number: 11623024
    Abstract: The invention relates to a cell sheet construct for neurovascular reconstruction. The cell sheet construct has a vascular endothelial cell layer and a neural stem cell layer, and the two layers are physically in direct contact with each other, where the vascular endothelial cell layer forms branching vasculatures, and the neural stem cell layer differentiates into neurons. The invention also relates to a method for manufacturing the cell sheet construct, having the following steps: culturing vascular endothelial cells on a substrate to form a vascular endothelial cell layer, seeding neural stem cells on the vascular endothelial cell layer to make the neural stem cells be physically in direct contact with the vascular endothelial cell layer, and culturing the neural stem cells and the vascular endothelial cell layer to differentiate into neurons and branching vasculatures to form a cell sheet construct.
    Type: Grant
    Filed: December 6, 2019
    Date of Patent: April 11, 2023
    Assignee: NATIONAL DEFENSE MEDICAL CENTER
    Inventors: Chung-Hsing Chou, Dueng-Yuan Hueng, Tsung-Neng Tsai
  • Patent number: 11597199
    Abstract: A group of inventions relates to biotechnology. Disclosed are a printing head and a printing device with fabric spheroids. The printing head includes a system of channels containing input and output channels, upper and lower channels for separation of nutrient medium, as well as a channel for a supporting plunger. Inlet channel has diameter providing movement of spheroid on it only by one. Output channel has diameter not less than diameter of inlet channel, includes input hole for input of printing tool and outlet hole for output of spheroids. Separation channels are made with possibility of connection of nutrient removal device with outlet channel through system of microchannels. Device includes a printing head, a device for feeding spheroids with a nutrient medium into an inlet channel, a device for removal of nutrient medium, a supporting plunger, a printing tool, a system for recording position of the spheroid in the output channel and a computing device for control.
    Type: Grant
    Filed: November 8, 2019
    Date of Patent: March 7, 2023
    Assignee: VIVAX BIO, LLC
    Inventors: Vladislav Parfenov, Frederico David Alencar de Sena Pereira, Yusef Khesuani, Vladimir Mironov, Elena Bulanova, Elizaveta Kudan, Alexander Ostrovskiy, Yakov Balakhovsky, Anastasiia Kniazeva, Stanislav Petrov
  • Patent number: 11566220
    Abstract: The disclosure relates to the establishment of a cell line having immune tolerance property using an optimal temperature profiling technique under a human body-like environment, and use thereof. The stem cell line exhibits immune tolerance property as they consecutively secret and express HLA-G proteins, and the culture medium of the stem cells contains a large amount of proteins capable of recovering various physiological functions and extracellular vesicles, and thus, the novel cell line or a culture medium thereof can be effectively used in various industries such as medicines and cosmetics.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: January 31, 2023
    Assignee: STEMMEDICARE CO., LTD.
    Inventor: Jang Ho Lee
  • Patent number: 11566278
    Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
    Type: Grant
    Filed: July 21, 2022
    Date of Patent: January 31, 2023
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 11559073
    Abstract: The substrates, systems, and methods described herein relate to textured substrates for preparing a comestible meat product. Substrates and methods are described herein for controlling one or more of growth, adhesion, retention, and/or release of cells (e.g., of a cell sheet) on or from the surface of the substrate. A method of preparing a comestible meat product may include applying a plurality of non-human cells to at least one patterned texture substrate, growing the cells on the patterned texture substrate to form the comestible meat product, and separating the comestible meat product from the patterned texture substrate. The patterned texture allows for improved growth, adhesion, retention, and/or release of cells as compared to another surface not comprising the patterned texture. In some embodiments, the cell culture substrate surfaces include a plurality of regions corresponding to a plurality of patterned textures.
    Type: Grant
    Filed: April 21, 2022
    Date of Patent: January 24, 2023
    Assignee: Upside Foods, Inc.
    Inventors: Matthew Leung, Asha Godbole, George C Engelmayr, Jr., Nicholas J Genovese, Uma S Valeti, Kathleen Carswell
  • Patent number: 11554373
    Abstract: A pumpless microfluidic system is disclosed that can be used to mimic the interaction of organ systems with the immune system. Also disclosed is a method for mimicking an immune system, comprising culturing a plurality of organ cells and at least one population of immune cells in the disclosed pumpless microfluidic system under physiological conditions. The method can further comprise activating an immune reaction in the pumpless microfluidic system, continuing the culture for a defined period, collecting a sample of culture medium from the system, and assaying the sample for one or more indicators of an immune response.
    Type: Grant
    Filed: December 29, 2017
    Date of Patent: January 17, 2023
    Assignees: University of Central Florida Research Foundation, Inc., Hesperos, Inc.
    Inventors: James Hickman, Alisha Colon, Christopher McAleer, Trevor Sasserath, John Rumsey, Daniel Elbrecht
  • Patent number: 11535825
    Abstract: Methods, compositions and cells are provided that identify and isolate a population of adult non-embryonic progenitor cells having multilineage potential, physical diameters of about 2 ?m to about 8 ?m in size or about 4 ?m to about 6 ?m, and expressing at least one of the stem cell associated genes among Oct-4, KLF-4, Nanog, Sox-2, Rex-1, GDF-3 or Stella. Methods are also provided that identify and isolate populations, which are subsets or subpopulations of progenitor adult stem cells within the population of the adult stem cells which is a heterogeneous population, the methods including contacting the adult stem cells with a ligand specific for at least one of: CD99, tetraspan, ICAM4, full-length MUC1, and truncated MUC1 receptor, in which a presence of a surface protein on the cells that bind to the ligand identifies the population which is the subset of the differentiated progenitor adult stem cells.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: December 27, 2022
    Assignee: TACS Bio Inc.
    Inventors: Keith D. Crawford, Baldev Vasir, John Garvey
  • Patent number: 11530380
    Abstract: A transfer device designed to extract an amorphous or semi-solid structure, tissue, or construct from supporting media while maintaining the spatial integrity/organizational architecture thereof. The transfer device can include a controller, an actuator assembly, a plunger, and a needle. The controller can move the transfer device and the plunger independently.
    Type: Grant
    Filed: January 4, 2021
    Date of Patent: December 20, 2022
    Assignee: DEKA Products Limited Partnership
    Inventors: Michael C. Tilley, Richard E. Andrews, Dane C. Fawkes
  • Patent number: 11498067
    Abstract: An apparatus for patterning biological cells, and a method of patterning and coculturing biological cells using the apparatus. The apparatus includes a fluidic structure having an outlet and a plurality of inlets, the fluidic structure is arranged to facilitate a flow of a plurality of different cells in a cell suspension therethrough, wherein each of the plurality of inlets is arranged to facilitate a loading of the plurality of different cells from a plurality of supplies into the fluidic structure; and a flow controlling device arranged to control the flow of the plurality of different cells through the fluidic structure and/or the loading of the plurality of different cells from the plurality of supplies through the plurality of inlets; wherein the fluidic structure is further arranged to facilitate a simultaneous observation of the plurality of different cells arranged in a predetermined pattern in the fluidic structure.
    Type: Grant
    Filed: January 7, 2021
    Date of Patent: November 15, 2022
    Assignee: City University of Hong Kong
    Inventors: Dong Sun, Lei Fan, Tao Luo
  • Patent number: 11466249
    Abstract: Provided is a cell tissue producing method including: a cell adhesive portion forming step of forming a cell adhesive portion formed of a cell adhesive material and having a predetermined shape at a predetermined position over a substrate having a cell non-adhesive surface; and a cell locating step of discharging a cell suspension containing at least cells and a cell drying inhibitor onto the cell adhesive portion in the form of a liquid droplet to locate the cells.
    Type: Grant
    Filed: March 12, 2019
    Date of Patent: October 11, 2022
    Assignee: RICOH COMPANY, LTD.
    Inventors: Hidekazu Yaginuma, Naoki Satoh, Tomoyuki Aratani, Chihiro Kubo, Daisuke Takagi, Momoko Shionoiri, Natsuko Iwashita
  • Patent number: 11396537
    Abstract: The present invention relates to a polypeptide, a process for the production thereof and a use thereof. The polypeptide of the present invention has excellent adhesion effect and is highly stable in an aqueous solution. Endotoxins can be more easily removed from the product of the polypeptide.
    Type: Grant
    Filed: November 27, 2019
    Date of Patent: July 26, 2022
    Assignee: Shanxi Jinbo Bio-Pharmaceutical Co., Ltd.
    Inventor: Xia Yang
  • Patent number: 11390842
    Abstract: A cell culture apparatus for conducting cell culture in which a culture medium is supplied together with cells to a container formed of a flexible material, wherein, a base having a flat mounting surface that holds the container is provided, partition pieces that can be protruded from the mounting surface for a prescribed height are embedded in the base, and when the partition pieces are accommodated into the base, upper end surfaces of the partition pieces are flushed with the mounting surface.
    Type: Grant
    Filed: June 18, 2020
    Date of Patent: July 19, 2022
    Assignee: TOYO SEIKAN GROUP HOLDINGS, LTD.
    Inventors: Norihiko Hata, Masahiro Murai
  • Patent number: 11376347
    Abstract: Provided are elastin-rich bioscaffolds, enhanced via stem cell pre-seeding and flow-based mechanical conditioning followed by subsequent removal of stem cells. The bioscaffolds may accelerate cardiovascular tissue regeneration and can be used in the surgical repair/reconstruction in various cardiovascular and other surgical applications including heart valves, cardiovascular grafts, and patches. The provided acellular, elastin-rich bioscaffold will pose minimal immune risk and reduced FDA regulatory hurdles as a non-living biologic product.
    Type: Grant
    Filed: June 21, 2021
    Date of Patent: July 5, 2022
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Sharan D. Ramaswamy, Brittany Gonzalez, Ariadna Herrera, Alexander Williams
  • Patent number: 11345841
    Abstract: A fiber silica composite microsphere for a shale stratum comprises a hollow silica sphere and fibers, and the fibers are partially coated on an outer surface of the hollow silica sphere and partially embedded into an interior of the hollow silica sphere. The hollow silica sphere has an outer diameter of 1-5 ?m and an inner diameter of 0.8-4.7 ?m; the fibers have a length of 5-10 ?m and a width of 1-3 ?m.
    Type: Grant
    Filed: October 19, 2021
    Date of Patent: May 31, 2022
    Assignee: Southwest Petroleum University
    Inventors: Yang Bai, Lingfeng Wu, Pingya Luo, Wenzhe Li, Feng Dai, Gang Xie, Haibo Liang, Kesheng Rong, Jiading Deng
  • Patent number: 11318224
    Abstract: Coated and expanded, nanofiber structures are provided and methods of use thereof.
    Type: Grant
    Filed: September 28, 2017
    Date of Patent: May 3, 2022
    Assignee: BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA
    Inventors: Jingwei Xie, Shixuan Chen, Mark Carlson
  • Patent number: 11154409
    Abstract: A percutaneous collar is made up of a central rigid ring and a flexible mesh inside a microporous silicone disc. The volume of the disc has a three-dimensional network of interconnected micropores forming microchannels connecting both external faces of the disc through the external micropores to the internal flexible mesh wherein the flexible mesh is formed by crossed longitudinal and radial elements or plates which form a plurality of holes.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: October 26, 2021
    Assignees: UNIVERSITAT POLITÉCNICA DE VALÈNCIA, UNIVERSITAT DE VALÈNCIA, FUNDACIÓN PARA LA INVESTIGACIÓN DEL HOSPITAL CLÍNICO DE LA COMUNIDAD VALENCIANA (INCLIVA)
    Inventors: José Expósito Ollero, Ana Vallés Lluch, José Albelda Vitoria, Juan Victor Hoyos Fuentes, Antonio Silvestre Muñoz
  • Patent number: 11149241
    Abstract: Cell culture devices, and related methods and kits, for modeling isotropic-to-anisotropic cellular transitions are provided. The devices can include a substrate having an isotropic film surface with one or more regions of aligned fibers dispersed thereon.
    Type: Grant
    Filed: February 21, 2019
    Date of Patent: October 19, 2021
    Assignee: Rensselaer Polytechnic Institute
    Inventors: Christopher John Rivet, Gregory Patrick Desmond, Jonathan Michael Zuidema, Ryan James Gilbert
  • Patent number: 11149252
    Abstract: The present disclosure aims to provide a method of efficiently manufacturing a cell mass, a cell structure, or a three-dimensional tissue body using a culturing surface coated with a temperature-responsive polymer or a temperature-responsive polymer composition. The manufacturing method of a cell mass, a cell structure, or a three-dimensional tissue body of the present disclosure includes seeding and culturing cells on a culturing surface coated with a temperature-responsive polymer or a temperature-responsive polymer composition.
    Type: Grant
    Filed: January 27, 2017
    Date of Patent: October 19, 2021
    Assignee: NATIONAL CEREBRAL AND CARDIOVASCULAR CENTER
    Inventors: Yasuhide Nakayama, Ryosuke Iwai, Yasushi Nemoto
  • Patent number: 11135589
    Abstract: Large bioreactors based on microfluidic technology, and methods of manufacturing the same, are provided, The big microbioreactor can include a chip or substrate having the microfluidic channels thereon, and the chip can be manufactured by forming a master mold, forming a male mold from a photopolymer plate using replica molding with the Fmold, and transferring features of the male to a polymer material.
    Type: Grant
    Filed: December 22, 2020
    Date of Patent: October 5, 2021
    Assignee: THE FLORIDA INTERNATIONAL UNIVERSITY BOARD OF TRUSTEES
    Inventors: Shekhar Bhansali, Maximiliano S. Perez, Betiana Lerner, Natalia Bourguignon
  • Patent number: 11046931
    Abstract: An aqueous cell culture medium composition includes an aqueous cell culture solution configured to support the culture of mammalian cells. The composition further includes a synthetic polymer conjugated to a polypeptide dissolved in the aqueous cell culture solution. The synthetic polymer conjugated to a polypeptide is configured to attach to the surface of a cell culture article under cell culture conditions. Incubation of the aqueous cell culture medium composition on a cell culture surface under cell culture conditions results is attachment to the surface of the synthetic polymer conjugated to the polypeptide.
    Type: Grant
    Filed: April 1, 2019
    Date of Patent: June 29, 2021
    Assignee: Coming Incorporated
    Inventors: Stephen Joseph Caracci, David Henry, Jessica Jo Kelley, Mark Alan Lewis, Yue Zhou
  • Patent number: 10920190
    Abstract: The present disclosure provides methods and systems for generating biological tissues that are configured for folding into a pre-determined three-dimensional form. The present disclosure utilizes contractile cells for folding a biological tissue into a three-dimensional shape. The methods include disposing a pattern of contractile cells on a surface that includes fibers actuated by the contractile cells and folding of the surface by the action of the contractile cells on the fibers. Tissues generated using the methods and systems of the present disclosure are also provided.
    Type: Grant
    Filed: May 3, 2017
    Date of Patent: February 16, 2021
    Assignee: The Regents of the University of California
    Inventors: Zev Jordan Gartner, Alex James Hughes
  • Patent number: 10875891
    Abstract: Disclosed herein are synthetic peptides and compositions comprising the same for the treatment and/or prophylaxis of a disease or a condition related to dry eye syndrome, psoriasis vulgaris or multiple myeloma. Also disclosed herein are methods of treating and/or preventing a disease or a condition related to dry eye syndrome, psoriasis vulgaris or multiple myeloma, by administering to a subject in need of such treatment a composition containing a therapeutically effective amount of a synthetic peptide of the present disclosure.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: December 29, 2020
    Assignee: MACKAY MEMORIAL HOSPITAL
    Inventors: Yeou-Ping Tsao, Tsung-Chuan Ho
  • Patent number: 10860001
    Abstract: A method of designing and manufacturing a replica composite object based on an original object. The method identifies the structure and physical properties of an original object. Base materials, bodies, and structural templates, each of which includes associated physical properties, are utilized to generate a 3-dimensional model. The 3-dimensional model is discretized and tested to determine if the selected combination of base materials and bodies have physical properties that substantially equal the physical properties of the original object. If the physical properties do not equate, the 3-dimensional model is optimized by adjusting the combination of base materials, bodies, and structural templates. When the difference between the measured physical properties of the 3-dimensional model and the identified physical properties of the original object is less than a tolerance value, the method instructs an additive manufacturing system to generate a replica composite object based on the original object.
    Type: Grant
    Filed: February 28, 2020
    Date of Patent: December 8, 2020
    Assignee: University of Central Florida Research Foundation, Inc.
    Inventors: Fluvio Lobo Fenoglietto, Jack Stubbs
  • Patent number: 10836987
    Abstract: A microfluidic device for determining a response of cells comprises a microchannel and a seeding channel. The microchannel is at least partially defined by a porous membrane having cells adhered thereto. The microchannel has a first cross-sectional area. The seeding channel delivers a working fluid to the cells within the microchannel. The seeding channel has a second cross-sectional area that is less than the first cross-sectional area such that a flow of the working fluid produces a substantially higher shear force within the seeding channel to inhibit the attachment of cells within the seeding channel. And when multiple seeding channels are used to deliver fluids to multiple microchannels that define an active cellular layer across the membrane, the seeding channels are spatially offset from each other such that fluid communication between the fluids occurs only at the active region via the membrane, not at the seeding channels.
    Type: Grant
    Filed: April 8, 2016
    Date of Patent: November 17, 2020
    Assignee: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
    Inventors: Karel Domansky, Christopher David Hinojosa, Donald E. Ingber, Daniel Levner, Guy Thompson, II
  • Patent number: 10829726
    Abstract: The pressure and circulation culture apparatus includes a holder (culture chamber part), a pressure transmission part and a culture solution circulation line (culture circuit). The holder is a means that reserves culture solution and that holds a cultured object. The pressure transmission part is a means that communicates with the holder and transmits an external pressure to the culture solution. The culture solution circulation line is a means that is connected to the holder via the pressure transmission part, and circulates the culture solution through the holder via the pressure transmission part. Such a structure is included, and an external pressure is made to operate on the culture solution via the pressure transmission part to allow pressure on the cultured object in the holder and circulation of the culture solution in the holder.
    Type: Grant
    Filed: July 27, 2017
    Date of Patent: November 10, 2020
    Assignee: PURPOSE CO., LTD.
    Inventors: Setuo Watanabe, Toshimi Murata, Mituharu Satou, Ibuki Kinouchi, Hidetada Takai
  • Patent number: 10793819
    Abstract: A liquid injection method for injecting a liquid into a culture vessel includes tilting the culture vessel around a horizontal axis at a tilt angle (X°) of greater than 0° and 50° or less, wherein adherent cells are adhered to the culture vessel; and injecting the liquid into the culture vessel at a predetermined linear velocity (Y mm/s) via a wall surface of the culture vessel tilted at the tilt angle (X°), wherein the tilt angle (X) and the linear velocity (Y) satisfy the following (formula 1): Y?5.075X+123 (formula 1).
    Type: Grant
    Filed: December 21, 2017
    Date of Patent: October 6, 2020
    Assignees: KANEKA CORPORATION, KYOTO UNIVERSITY
    Inventors: Tomohisa Kato, Kazuya Hamada, Haruhisa Inoue, Takayuki Kondo
  • Patent number: 10732172
    Abstract: A method is directed to determining a thrombosis function and includes flowing a fluid sample over a surface having a fixed endothelial cell monolayer. The method further includes stimulating the fixed endothelial cell monolayer to induce formation of a clot, the clot being formed via interaction between the fixed endothelial cell monolayer and the fluid sample. In response to the clot formation, the method further includes determining a thrombosis function associated with the fluid sample and the fixed endothelial cell monolayer.
    Type: Grant
    Filed: May 22, 2016
    Date of Patent: August 4, 2020
    Assignees: President and Fellows of Harvard College, Children's Medical Center Corporation
    Inventors: Donald E. Ingber, Abhishek Jain, Andries D. van der Meer, Alan David Michelson, Andrew L. Frelinger, III, Riccardo Barrile
  • Patent number: 10702627
    Abstract: This invention provides aragonite- and calcite-based scaffolds for the repair, regeneration, enhancement of formation or a combination thereof of cartilage and/or bone, which scaffolds comprise at least two phases, wherein each phase differs in terms of its chemical content, or structure, kits comprising the same, processes for producing solid aragonite or calcite scaffolds and methods of use thereof.
    Type: Grant
    Filed: July 30, 2018
    Date of Patent: July 7, 2020
    Assignee: Cartiheal (2009) Ltd.
    Inventors: Nir Altschuler, Razi Vago
  • Patent number: 10668467
    Abstract: A microfluidic device includes a substrate, a cover layer and one or more chambers disposed within the cover layer, the substrate or both. Each chamber has a first end, a second end, and a set of micro-pillars disposed therein. A first microchannel and second microchannel are disposed within the cover layer, the substrate or both, and connected to the first end and second end of the chamber, respectively. A first set of barriers is disposed within each first microchannel proximate to the first end of the chamber. A second set of barriers is disposed within each second microchannel proximate to the second end of the chamber. A third microchannel is disposed within the cover layer, the substrate or both, and connected to the chamber. A first port, second port and third port extend through the cover layer and connect to the first, second and third microchannels, respectively.
    Type: Grant
    Filed: June 7, 2016
    Date of Patent: June 2, 2020
    Assignee: Texas Tech University System
    Inventors: Siva A. Vanapalli, Mizanur Rahman
  • Patent number: 10533158
    Abstract: The problem to be solved by the present invention is to provide a cultured cell sheet that expresses phenotypes of the chondroid tissue. The problems can be solved by producing the cultured cell sheet in which cells are cultured on a surface of a cell cultureware that is coated with a temperature responsive polymer having an upper or lower critical solution temperature ranging from 0° C. to 80° C. in water, including steps of: adjusting the temperature of the culture medium to a temperature below the lower critical solution temperature or above the upper critical solution temperature; bringing the cultured cell sheet in close contact with the carrier; and detaching the cultured cell sheet together with the carrier.
    Type: Grant
    Filed: February 28, 2006
    Date of Patent: January 14, 2020
    Assignees: TOKAI UNIVERSITY EDUCATIONAL SYSTEM, CELLSEED, INC.
    Inventors: Masato Sato, Hideaki Sakai
  • Patent number: 10071187
    Abstract: Described are methods, cell growth substrates, and devices that are useful in preparing cell-containing graft materials for administration to patients. Tubular passages can be defined in cell growth substrates to promote distribution of cells into the substrates. Also described are methods and devices for preparing cell-seeded graft compositions, methods and devices for preconditioning cell growth substrates prior to application of cells, and cell seeded grafts having novel substrates, and uses thereof.
    Type: Grant
    Filed: August 25, 2015
    Date of Patent: September 11, 2018
    Assignees: Cook Biotech Incorporated, Muffin Incorporated
    Inventors: Michael C. Hiles, Chad E. Johnson, Neal E. Fearnot, Thomas Payne, Ronald Jankowski
  • Patent number: 10004830
    Abstract: The present invention relates to a three-dimensional cell culture system which can be used to culture a variety of different cells (Human mesenchymal stem cells, Human fibroblast, Keratinocytes, fibroblast differentiated form mesenchymal stem cells in vitro and keratinocytes differentiated form mesenchymal stem cells in vitro) in vitro for continued periods of time. In the present invention, mesenchymal stem cells (MSCs) derived from bone marrow or umbilical cord are inoculated and grown on a pre-established scaffold or matrix made up of chitosan and gelatin. The developed tissue constructs contain cells, growth factors, cytokines and other regulatory factors secreted by cells. The developed bioengineered tissue constructs provide the support, growth factors and essential cytokines for wound healing. Developed tissue construct useful in the treatment of burns wound, diabetic foot ulcer wound and type of chronic skin wounds. It is helpful in healing and regeneration of skin tissue.
    Type: Grant
    Filed: April 12, 2017
    Date of Patent: June 26, 2018
    Assignee: DATT MEDIPRODUCTS LIMITED and DATT LIFE SCIENCE PVT. LTD.
    Inventors: Rajan Datt, Siddharth Pandey, Pallavi Shrivastava, Supriya Srivastava
  • Patent number: 9962464
    Abstract: Provided are injectable, hyaluronic acid-based dermal filler compositions including conjugated vitamin C derivatives. The compositions provide for extended release of active vitamin C into tissue, promoting collagenesis and other benefits to skin.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: May 8, 2018
    Assignee: Allergan, Inc.
    Inventors: Futian Liu, Xiaojie Yu, Jessamine P. Winer-Jones, Nicholas J. Manesis, Hengli Liu, Gabriel Njikang, Athene Wan Chie Chan
  • Patent number: 9913700
    Abstract: The present invention relates to a biomimetic matrix for providing structural support and scaffolding that allows for regeneration of dentin, pulp, and periodontal tissues. A method of making the biomimetic matrix provides the ability to select both a size of a pore or tubule formed in the biomimetic matrix and a density of pores or tubules disposed throughout the biomimetic matrix. The present invention discloses an approach of successful tubular dentin regeneration both in vitro and in vivo using the biomimetic matrix.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: March 13, 2018
    Assignee: The Texas A&M University System
    Inventors: Xiaohua Liu, Chi Ma, Tiejun Qu