Support Is A Gel Surface Patents (Class 435/397)
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Publication number: 20130260464Abstract: The present invention relates to a new method for treating or processing a cross-linked hyaluronan hydrogel prior to its use in applications involving three-dimensional cell culture. The invention also relates to cross-linked hyaluronan hydrogels prepared by such a method and to their use in 3D cell culture, stem cell/tissue engineering, drug discovery, toxicology testing, and broad cell biology applications.Type: ApplicationFiled: June 22, 2011Publication date: October 3, 2013Inventors: Jean-Pierre Vannier, Laurent David, Didier Lecerf, Virginie Dulong, Berenice Coquerel, Elise Demange
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Patent number: 8541235Abstract: A modified reconstituted extracellular matrix composition is provided herein. The composition includes an extracellular matrix and at least one exogenous component selected from heparin, fibronectin and laminin. The composition may have a basic pH. Additionally, provided herein is a cell culturing system including a substrate and a coating thereon of the composition to assess potential stimulators and/or inhibitors for their effects on various cell cultures while increasing the signal dynamic range.Type: GrantFiled: September 28, 2009Date of Patent: September 24, 2013Assignee: Corning IncorporatedInventors: Min Wu, Frank J. Mannuzza
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Publication number: 20130236971Abstract: Disclosed herein are biodegradable hydrogel scaffolds for use in tissue engineering. The hydrogel scaffolds are composed of synthetic terpolymers complexed with polyvinyl alcohol (PVA), which facilitate cell-sheet and tissue growth. In the presence of a monosaccharide, the PVA-hydrogel is dissolved and cell-sheets are released for harvesting. Further disclosed herein are methods for producing PVA hydrogels which support tissue growth. Tissue engineering applications and methods are also disclosed.Type: ApplicationFiled: August 8, 2011Publication date: September 12, 2013Applicant: INDIAN INSTITUTE OF TECHNOLOGY KANPURInventor: Ashok Kumar
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Publication number: 20130238092Abstract: A composite comprising a stem cell; a biodegradable layer, which can provide an environment for the stem cell to grow and to differentiate, and; a N-isopropylacrylamide (NIPAAm), which can polymerize with the biodegradable layer and possess the temperature-responsive character for easy stripping. The present invention further provides a method for treating a patient with a skin defect, consisting of (a) providing said patient with a composite consisting of a N-isopropylacrylamide (NIPAAm) layer polymerized with a biodegradable layer containing gelatin and a layer of polypropylene (PP) non-woven, wherein a bone marrow derived mononuclear cell with CD45 negative and glycophorin A negative is cultivating on the biodegradable layer; (b) covering said composite on the skin defect of the patient; and (c) treating the composite with water below 25° C. to strip off the layer of polypropylene (PP) non-woven.Type: ApplicationFiled: April 17, 2013Publication date: September 12, 2013Applicant: Taipei Veterans General HospitalInventors: Shih-Hwa Chiou, Cherng-Kang Perng, Han-Tzo Lin, Yi-Ping Yang
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Publication number: 20130217127Abstract: A cryogel contains a polyol and a co-polymer of Formula (I).Type: ApplicationFiled: August 8, 2011Publication date: August 22, 2013Inventor: Ashok Kumar
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Publication number: 20130217126Abstract: A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.Type: ApplicationFiled: August 25, 2011Publication date: August 22, 2013Inventors: Toshiaki Takezawa, Ayumi Oshikata, Hiroyuki Kuroyama, Tomoya Sawaguchi, Hiroyuki Yamaguchi
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Publication number: 20130210147Abstract: Use of the amino acid sequence Har-Gly-Asp (hRGD) as a bioactive sequence in functional peptides to promote cell adhesion, cell growth, and/or cell differentiation, and in the preparation of hydrogels, preferably hydrogels for cell culture. A hydrogel comprising the hRGD sequence, especially a hydrogel wherein the hRGD sequence is part of the hydrogel scaffold.Type: ApplicationFiled: October 6, 2011Publication date: August 15, 2013Applicant: SOLVAY SAInventors: Laurent Jeannin, Roland Callens, Wafa Moussa
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Publication number: 20130196435Abstract: A method for producing highly uniform cell colonies in a cell culture dish with the use of stencils made from an elastomeric sheet sized to fit within the cell culture dish, having a singular opening or a plurality of openings of a number, pitch and diameter configured to optimally control the geometric growth parameters of a cell colony. The uniform cell colonies are produced by placing the stencil in a cell culture dish and hydropilizing the stencil. The stencil is overlayed with cell culture media and seeded with seed cells that are preferably grown for at least a day before the stencil is removed to produce a pattern of seeded cells with controlled pitch, colony diameter and density within the culture dish that grow to become highly uniform cell colonies. A kit with culture dish, stencil, culture media and growth media is also provided.Type: ApplicationFiled: January 10, 2013Publication date: August 1, 2013Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: THE REGENTS OF THE UNIVERSITY CALIFORNIA, THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY
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Publication number: 20130189723Abstract: The present invention relates generally to the field of cell culture, which is a laboratory process used primarily for the growth, propagation, and production of cells for analysis and the production and harvesting of cell products. The present invention comprises functionalized and/or engineered hydrogel microcarriers that exhibit any or all of the following properties: controllable buoyancy, ferro- or paramagnetism, molecular or fabricated reporting elements, and optical clarity. The microcarriers are used in a bioreactor that employs external forces to control said microcarrier kinetic energy and translational or positional orientation in order to facilitate cell growth and/or cellular analysis. The bioreactor can be part of an automated system that employs any or all of the following; a microcarrier manufacturing method, a monitoring method, a cell culture method, and an analytical method.Type: ApplicationFiled: September 26, 2012Publication date: July 25, 2013Applicant: Global Cell Solutions, LLCInventor: Global Cell Solutions, LLC
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Publication number: 20130149782Abstract: The present invention relates to collagen hydrogels. Particularly, the invention relates to hydrogels comprising a telopeptide collagen (“telo-collagen”) and an atelopeptide collagen (“atelo-collagen”); hydrogels comprising collagen and chitosan; methods of making the hydrogels; methods of reducing gelation of a hydrogel mixture at room temperature; methods of reducing compaction of cells; and methods of culturing cells on such hydrogels.Type: ApplicationFiled: December 9, 2011Publication date: June 13, 2013Applicant: MEDTRAIN TECHNOLOGIES, LLCInventors: Albert J. Banes, Mari Tsuzaki, Jie Qi
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Patent number: 8450108Abstract: A device, and method of making the device, capable of therapeutic treatment and/or for in vitro testing of human skin. The device may be used on skin wounds for burned, injured, or diseased skin, and provides structures and functions as in normal uninjured skin, such as barrier function, which is a definitive property of normal skin. The device contains cultured dermal and epidermal cells on a biocompatible, biodegradable reticulated matrix. All or part of the cells may be autologous, from the recipient of the cultured skin device, which advantageously eliminates concerns of tissue compatibility. The cells may also be modified genetically to provide one or more factors to facilitate healing of the engrafted skin replacement, such as an angiogenic factor to stimulate growth of blood vessels.Type: GrantFiled: June 18, 2010Date of Patent: May 28, 2013Assignees: University of Cincinnati, Shriners Hospitals for ChildrenInventor: Steven T. Boyce
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Patent number: 8450105Abstract: A process for making a gel comprising combining a silanol species comprising at least two silanol groups per molecule and a hydrophilic hydroxyl species comprising at least two hydroxyl groups per molecule. The gel is capable of being converted to a liquid by application of a mechanical shear force and the liquid is capable of being converted to the gel in the absence of the mechanical shear force.Type: GrantFiled: May 4, 2007Date of Patent: May 28, 2013Assignee: Agency for Science, Technology and ResearchInventors: Jackie Y. Ying, Shona Pek, Andrew C A Wan
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Patent number: 8449902Abstract: This invention relates to the production of biomaterials with variable stiffness by subjecting a gel which varies in at least one dimension to plastic compaction. These biomaterials may be useful, for example, for the directional control or guidance of cells within tissue equivalent implants.Type: GrantFiled: July 7, 2008Date of Patent: May 28, 2013Assignee: UCL Business PLCInventors: Robert Brown, Ektors Hadjipanayi
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Publication number: 20130130384Abstract: A temperature responsive sheet is disclosed that comprises a chemically modified water-soluble elastin obtained by N-acylating at least some of the primary amines and secondary amines contained in a high molecular weight water-soluble elastin molecule and coupling some or all of carboxyl groups contained in the molecule with a glycine alkyl ester. Also is disclosed a process for producing a cell sheet that comprises preparing above the temperature responsive sheet a film that functions as a scaffold for animal cells, culturing specific cells on the film so as to prepare a cell sheet, and subsequently separating the cell sheet and the temperature responsive sheet comprising the chemically modified water-soluble elastin under conditions of no greater than the culturing temperature for the cells.Type: ApplicationFiled: June 9, 2011Publication date: May 23, 2013Applicant: KYUSHU INSTITUTE OF TECHNOLOGYInventors: Kouji Okamoto, Erika Yoshino
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Patent number: 8445281Abstract: Compositions and methods are provided for enhancing the survival and promoting the maturation of mammalian oocytes, zygotes and preimplantation embryos. BDNF or BDNF agonists may be administered to an individual, or to cells in vitro, to enhance cellular maturation, embryo growth and fertilization. Accordingly, compositions comprising BDNF are herein presented for use in promoting in vivo oocyte maturation as well as for use as a component in culture media for promoting preimplantation maturation of zygotes and embryos, for instance, for use with in vitro fertilization procedures and for the production of stem cells. Additionally, compounds that interfere with the binding of BDNF to its receptor may be administered to an individual to prevent oocyte maturation, thereby acting as a contraceptive. The BNDF receptor, TrkB, and BDNF also find use in the screening and design of agonists and antagonists for use in the methods of the invention.Type: GrantFiled: June 17, 2010Date of Patent: May 21, 2013Assignee: The Board of Trustees of the Leland Stanford Junior UniversityInventors: Kazuhiro Kawamura, Aaron J. W. Hsueh, Sabine M. Mulders
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Patent number: 8445280Abstract: A method for creating networks of perfusable microvessels in vitro. Cells including cell types capable of sprouting are seeded 1300 into a channel in a matrix at to activate competency 1304 of the cells for sprouting as microvessels based on the seeding density. The matrix channel is perfused with medium to allow parent vessels to form and for viability 1324. The parent vessels and matrix are incubated and perfused to provide for sprouting of microvessels from parent vessels into the surrounding matrix 1328. The sprouting parent vessels are grown until network forms 1332.Type: GrantFiled: September 24, 2008Date of Patent: May 21, 2013Assignee: Nortis, Inc.Inventors: Thomas Neumann, Anna Tourovskaia, Mark E. Fauver, Julia Oi Yan Yu
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Patent number: 8415173Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.Type: GrantFiled: August 26, 2011Date of Patent: April 9, 2013Assignee: Crystal Bioscience Inc.Inventor: William Don Harriman
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Publication number: 20130084638Abstract: A scaffold for vascular endothelial cell migration includes a recombinant gelatin having an amino acid sequence derived from a partial amino acid sequence of collagen. A method for producing a blood vessel uses this scaffold.Type: ApplicationFiled: August 29, 2012Publication date: April 4, 2013Applicant: FUJIFILM CORPORATIONInventors: Reiko IWAZAWA, Kentaro NAKAMURA
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Patent number: 8404485Abstract: A coated microcarrier for cell culture includes a microcarrier base and a polymeric coating grafted to the base via a polymerization initiator. A method for forming the coated microcarrier includes (i) conjugating a polymerization initiator to the microcarrier base to form an initiator-conjugated microcarrier base; (ii) contacting the initiator-conjugated microcarrier base with monomers; and (iii) activating the initiator to initiate polymerization and graft the polymer to the base.Type: GrantFiled: July 25, 2012Date of Patent: March 26, 2013Assignee: Corning IncorporatedInventors: Kevin Robert McCarthy, Simon Kelly Shannon, Florence Verrier
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Patent number: 8404484Abstract: Substrates for cell culture and tissue engineering bioreactors consisting of polymers that change their shape over time under stimulation by temperature change, hydration, degradation, or other means. A method of controlling cell culture using a biodegradable shape memory polymer, wherein shape changes can transfer stresses, strains, or both to adherent or otherwise connected cells such that the mechanical stimulus impacts cell development and the resulting properties of tissues.Type: GrantFiled: July 15, 2010Date of Patent: March 26, 2013Assignee: Syracuse UniversityInventors: Patrick Mather, James Henderson, Kelly Burke, Kevin Davis, Xiuling Xu
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Publication number: 20130053324Abstract: There is described a group of novel self-assembling peptides (SAPs), comprising biotinylated and unbiotinylated sequences, hybrid peptide-peptoid sequences, branched sequences for a total of 48 tested motifs, showing a heterogeneous ensemble of spontaneously self-assembled structures at the nano- and microscale, ranging from short tabular fibers to twisted ribbons, nanotubes and hierarchical self-assembled micrometer-long sheets. Specifically, the SAPs according to the present invention which initially spontaneous assemble, surprisingly form stable solid scaffolds upon exposure to neutral pH buffer. Further these SAPs allow adhesion, proliferation and differentiation of murine and human neural stem cells and have self-healing propensity. They also did not exert toxic effects in the central nervous system, can stop bleeding and foster nervous regeneration.Type: ApplicationFiled: April 19, 2011Publication date: February 28, 2013Applicant: UNIVERSITA' DEGLI STUDI DI MILANO BICOCCAInventors: Angelo Luigi Vescovi, Fabrizio Gelain
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Patent number: 8367809Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 30, 2009Date of Patent: February 5, 2013Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Publication number: 20120329156Abstract: A scaffold having a reinforced tissue regeneration ability and a method of manufacturing the same are provided. The scaffold is formed in a lattice form by alternately stacking biodegradable synthetic polymer-hydrogel layers. In this case, the biodegradable synthetic polymer-hydrogel layer is formed by disposing a plurality of biodegradable synthetic polymer-hydrogel units including a biodegradable synthetic polymer and a hydrogel at a predetermined gap.Type: ApplicationFiled: March 4, 2011Publication date: December 27, 2012Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATIONInventors: Dong-Woo Cho, Jong Young Kim, Jin-Hyung Shim
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Publication number: 20120329157Abstract: It is an object of the present invention to provide a cell three-dimensional construct that has a thickness sufficient for tissue regeneration and comprises cells uniformly distributed therein. The present invention provides a cell construct comprising polymer blocks having biocompatibility and cells, wherein the plural polymer blocks are arranged in spaces between the plural cells.Type: ApplicationFiled: March 1, 2011Publication date: December 27, 2012Applicant: FUJIFILM CORPORATIONInventor: Kentaro Nakamura
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Publication number: 20120301963Abstract: A microfiber showing improved mechanical strength, which comprises a micro gel fiber consisting of collagen gel or the like covered with high strength hydrogel such as alginate gel.Type: ApplicationFiled: October 12, 2010Publication date: November 29, 2012Applicant: THE UNIVERSITY OF TOKYOInventors: Shoji Takeuchi, Hiroaki Onoe, Yukiko Matsunaga, Daisuke Kiriya, Riho Gojo, Midori Negishi
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Patent number: 8318492Abstract: The present invention relates in one aspect to a method for determining the cell culture history of a cell unit labelled with more than one type of tag comprising the steps of: (a) measuring one or more parameters of each tag that is used to label the cell unit; (b) identifying each tag in the cell unit; and (c) correlating the identity of each tag to the identity of the cell unit and/or the specific cell culture conditions to which the cell unit has been exposed.Type: GrantFiled: February 25, 2008Date of Patent: November 27, 2012Assignee: Plasticell LimitedInventors: Yen Choo, Fraser Hornby, John Girdlestone
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Publication number: 20120282318Abstract: The present invention relates to a sheet for corneal transplants comprising corneal endothelial cells on a gelatin hydrogel, which is obtainable by seeding and culturing corneal endothelial cells on a gelatin hydrogel coated with collagen. The sheet of the present invention is extremely useful as a sheet for corneal transplants not only for its biocompatibility and biodegradability, but also for its high transparency.Type: ApplicationFiled: August 16, 2010Publication date: November 8, 2012Applicants: KOYOTO UNIVERSITY, TOHOKU UNIVERSITYInventors: Kohji Nishida, Ryuhei Hayashi, Ryo Watanabe, Yasuhiko Tabata
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Publication number: 20120231544Abstract: This invention describes a cell growth apparatus, particularly neuronal printed circuit board apparatus comprising an aerogel base and a pre-printed cellular growth pattern. The cellular growth pattern is comprised of combinations of layers of cellular adhesion promoting materials, cellular adhesion inhibiting materials, and/or cellular signal promoting materials. The invention further describes methods of promoting cell growth using the neuronal printed circuit board apparatus of the invention. The invention is useful for regeneration and precise guidance of cells, particularly nerve cells, when used as an implant.Type: ApplicationFiled: April 15, 2010Publication date: September 13, 2012Applicant: UNIVERSITY OF MEMPHIS RESEARCH FOUNDATIONInventor: Firouzeh Sabri
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Patent number: 8263405Abstract: To provide a new reductive-stimuli-responsive degradable gel that allows any control of decomposition of the three-dimensional base material for cell culture and production of a completely biological three-dimensional cellular structure consisting only of cells and cells-produced extracellular matrix and that allows safe recovery of the cellular structure produced. A stimuli-responsive hydrogel, characterized by being produced by crosslinking a water-soluble polymer with a compound having a disulfide bond in the molecular chain.Type: GrantFiled: November 24, 2006Date of Patent: September 11, 2012Assignees: NOF CorporationInventors: Mitsuru Akashi, Yoshiki Sawa, Michiya Matsusaki
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Publication number: 20120214238Abstract: A hydrogel tissue engineering scaffold having microbubbles dispersed therein is disclosed. Also, a system for cell culturing including a controller and actuator to apply dynamic deformational loading to a hydrogel is disclosed. Also disclosed are methods for producing hydrogels with microbubbles and for culturing cells using hydrogels with microbubbles.Type: ApplicationFiled: August 31, 2010Publication date: August 23, 2012Applicant: The Trustees of Columbia University in the City of New YorkInventors: Mark A. Borden, Eric G. Lima, Clark T. Hung, Shashank Ramesh Sirsi
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Publication number: 20120208276Abstract: The present disclosure relates to supports and scaffolds for cell and tissue engineering. The supports disclosed herein are composed of a thermally responsive material, containing pillars, that is coated with an acrylic polymer, thereby imparting an amphipathic matrix foundation. When exposed to a change in temperature, the coated support reacts by facilitating or repelling hydromolecular interactions. Further disclosed herein are methods for making hydrogels that can support tissue growth.Type: ApplicationFiled: February 16, 2011Publication date: August 16, 2012Inventor: Seth Miller
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Patent number: 8241905Abstract: A composition comprising a plurality of cell aggregates for use in the production of engineered organotypic tissue by organ printing. A method of making a plurality of cell aggregates comprises centrifuging a cell suspension to form a pellet, extruding the pellet through an orifice, and cutting the extruded pellet into pieces. Apparatus for making cell aggregates comprises an extrusion system and a cutting system. In a method of organ printing, a plurality of cell aggregates are embedded in a polymeric or gel matrix and allowed to fuse to form a desired three-dimensional tissue structure. An intermediate product comprises at least one layer of matrix and a plurality of cell aggregates embedded therein in a predetermined pattern. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type, matrix, and embedding patterns to enable selection of organ printing processes parameters for use in producing an engineered tissue having a desired three-dimensional structure.Type: GrantFiled: February 24, 2005Date of Patent: August 14, 2012Assignees: The Curators of the University of Missouri, MUSC Foundation for Research DevelopmentInventors: Gabor Forgacs, Karoly Jakab, Adrian Neagu, Vladimir Mironov
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Publication number: 20120178162Abstract: Synthetic cell culture surfaces, including a hydrophobe modified cellulose or an hydroxylated acrylate polymer composition and optionally including a silica source, cell culture coating and cell culture articles incorporating the composition, and methods of making and using the articles for cell culture, as defined herein.Type: ApplicationFiled: October 11, 2011Publication date: July 12, 2012Inventors: Wendy Annette Baker, Theresa Chang, Robert Randall Hancock, JR.
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Publication number: 20120171258Abstract: Scaleable, vascularised tissue constructs that are composed of a multiplicity of cell containing, discrete and separable modules, methods of fabricating same and uses thereof. The tissue construct is a tissue substitute used in tissue transplantation or substitution or for the purpose of in vitro mimic of normal tissue.Type: ApplicationFiled: February 29, 2012Publication date: July 5, 2012Inventors: Michael V. Sefton, Alison McGuigan
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Patent number: 8206982Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: October 14, 2008Date of Patent: June 26, 2012Assignee: Natrix Separations Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Publication number: 20120148543Abstract: The present invention relates to the use of a plastically-compacted collagen gel as a substrate for the growth of corneal cells, particularly limbal corneal epithelial stem cells. Cells grown on such a substrate can be cultured to produce artificial ocular epithelia which can be used in ocular toxicity testing or for transplantation.Type: ApplicationFiled: May 21, 2010Publication date: June 14, 2012Inventor: Che Connon
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Patent number: 8198083Abstract: An organotypic slice and a method of preparing an organotypic slice from a central nervous system tissue, wherein the organotypic slice comprises a brain slice obtained from a brain wherein mature synapses have not been established and the organotypic slice is seeded with a population of stem cells; wherein the organotypic slice has enhanced viability as compared to an organotypic slice comprising a similar brain slice not seeded with a population of stem cells.Type: GrantFiled: October 31, 2008Date of Patent: June 12, 2012Inventors: William Gunter Loudon, Shengwen Li, Brent A. Dethlefs
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Patent number: 8187880Abstract: This invention relates to a composite material that comprises a support member that has a plurality of pores extending through the support member and, located in the pores of the support member, and filling the pores of the support member, a macroporous cross-linked gel. The invention also relates to a process for preparing the composite material described above, and to its use. The composite material is suitable, for example, for separation of substances, for example by filtration or adsorption, including chromatography, for use as a support in synthesis or for use as a support for cell growth.Type: GrantFiled: April 23, 2008Date of Patent: May 29, 2012Assignee: Natrix Separations, Inc.Inventors: Ronald F. Childs, Carlos Filipe, Raja Ghosh, Alicja M. Mika, Jinsheng Zhou, Elena N. Komkova, Marcus Y. Kim, Tapan K. Dey
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Patent number: 8187852Abstract: A porous film is used as a cell culture substrate that is a scaffold for a spheroid. The porous film has a plurality of pores whose diameters gradually increase in a direction A. The diameters of the pores gradually increase from a first area P1 to a third area P3 in this order. The first to the third areas P1 to P3 are located on the porous film along the direction A. The diameters and depths of the pores increase in proportion to the distance in the direction A.Type: GrantFiled: March 26, 2009Date of Patent: May 29, 2012Assignee: FUJIFILM CorporationInventors: Hidekazu Yamazaki, Tsukasa Ishihara
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Publication number: 20120129207Abstract: Compositions and methods described herein provide a cell culture system in which cells are in high metabolic states from the onset of the culture. Combinations of various cell culture components disclosed and employed herein allow cells to be in high metabolic states useful for drug testing immediately after the start of cell culture.Type: ApplicationFiled: November 24, 2009Publication date: May 24, 2012Applicants: THE GENERAL HOSPITAL CORPORATION, HUREL CORPORATIONInventors: Martin Yarmush, Robert Freedman, Yaakov Nahmias, Erik Novik
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Publication number: 20120114617Abstract: Synthetic peptide including the sequence KKLRIKSKEK (SEQ ID 1) or a sequence of identical size, in which the K residue (in position 1) and the R residue (in position 4) are conserved, the sequence being able to bind to the syndecan-1 receptor.Type: ApplicationFiled: March 15, 2010Publication date: May 10, 2012Applicants: Symatese, Universite Claude Bernard Lyon I, Centre National De La Recherche ScientifiqueInventor: Patricia Rousselle
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Patent number: 8158425Abstract: A cell culture scaffold containing a gel having a network structure comprised of a synthetic polymer such that cultured cells spread in a shorter time and the number of adsorbed cultured cells per unit area is larger than in the case of using a gel having a network structure comprised of polyacrylic acid, while taking advantage of synthetic polymers with low manufacturing cost, easy quality control and no risk of virus infection in cultured cell. Used as the cell culture scaffold is a gel containing a synthetic polymer obtained by polymerization or copolymerization of a monomer having a sulfonic group such as p-styrenesulfonic acid sodium salt (NaSS) and 2-acrylamide-2-methylpropane sulfonic acid sodium salt (NaAMPS).Type: GrantFiled: August 29, 2008Date of Patent: April 17, 2012Assignee: Hokkaido UniversityInventors: Jian Ping Gong, Yoshihito Osada, Yongmei Chen
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Publication number: 20120058066Abstract: Provide are a peptide gel with practically sufficient mechanical strength and a self-assembling peptide capable of forming the peptide gel. The self-assembling peptide is formed of the following amino acid sequence: a1b1c1b2a2b3db4a3b5c2b6a4 where: a1 to a4 each represent a basic amino acid residue; b1 to b6 each represent an uncharged polar amino acid residue and/or a hydrophobic amino acid residue, provided that at least five thereof each represent a hydrophobic amino acid residue; c1 and c2 each represent an acidic amino acid residue; and d represents a hydrophobic amino acid residue.Type: ApplicationFiled: February 12, 2010Publication date: March 8, 2012Applicants: NATIONAL UNIV. CORPORATION OKAYAMA UNIV., MENICON CO., LTD.Inventors: Yusuke Nagai, Hidenori Yokoi, Koji Uesugi, Keiji Naruse
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Publication number: 20120058554Abstract: A cell culture polysaccharide microcarrier includes (1) a cross-linked polysaccharide microcarrier base having a neutral or negative charge at pH 7, and (ii) a polypeptide conjugated to the base. The polypeptide may contain a cell adhesive sequence, such as RGD. Cells cultured with such microcarriers exhibit peptide-specific binding to the microcarriers.Type: ApplicationFiled: July 28, 2010Publication date: March 8, 2012Inventors: Sophie Deshayes, David Henry, Martial Hervy
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Patent number: 8129188Abstract: A cell culture apparatus and a method for fabricating the cell culture apparatus are disclosed, the method comprises forming at least one fillister on a biomaterial composite layer by photolithography, wherein the biomaterial composite layer contains two gel materials. One is a bio-compatible hydrogel composition having various weight ratio of: 2-hydroxyethylmathacrylate (HEMA), bisphenol A and glycidyl methacrylate (bis-GMA), triethylene glycol dimethacrylate (TEGDMA), r-methacryloxypropyl trimethoxysilane (MAPTMS), ?,?-diethoxyacetophenone (DEAP), and the other one is a photo-sensitive silica gel composition.Type: GrantFiled: June 10, 2008Date of Patent: March 6, 2012Assignee: Industrial Technology Research InstituteInventors: Ming-Cheng Shih, Chin-Fu Chen, Rung-Jiun Gau, Yu-Shih Weng, Ya-Jen Yu, Shao-Jen Yeh
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Publication number: 20120052511Abstract: In one embodiment, an aerogel or xerogel includes column structures of a material having minor pores therein and major pores devoid of the material positioned between the column structures, where longitudinal axes of the major pores are substantially parallel to one another. In another embodiment, a method includes heating a sol including aerogel or xerogel precursor materials to cause gelation thereof to form an aerogel or xerogel and exposing the heated sol to an electric field, wherein the electric field causes orientation of a microstructure of the sol during gelation, which is retained by the aerogel or xerogel. In one approach, an aerogel has elongated pores extending between a material arranged in column structures having structural characteristics of being formed from a sol exposed to an electric field that causes orientation of a microstructure of the sol during gelation which is retained by the elongated pores of the aerogel.Type: ApplicationFiled: July 11, 2011Publication date: March 1, 2012Applicant: LAWRENCE LIVERMORE NATIONAL SECURITY, LLCInventors: Marcus A. Worsley, Theodore F. Baumann, Joe H. Satcher, JR., Tammy Y. Olson, Joshua D. Kuntz, Klint A. Rose
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Publication number: 20120029416Abstract: The present invention provides porous electroactive hydrogels, the deformation angle of which is controlled by electroactuation, and methods for preparing and using such hydrogels.Type: ApplicationFiled: September 24, 2009Publication date: February 2, 2012Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: Kevin Kit Parker, Megan O'Grady
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Publication number: 20120015440Abstract: A spheroid composite includes: a substrate including a cell-adhesive porous base material and plural hydrophilic regions and hydrophobic regions that are disposed on the porous base material and formed by curing a photosensitive composition, wherein the photosensitive composition includes a branched polyalkylene glycol derivative having three or more polyalkylene glycol groups, each having a polymerizable substituent at a terminal thereof, and a tri- or higher-valent linking group that binds to the polyalkylene glycol groups; and spheroids formed in the hydrophobic regions on the substrate, the plural spheroids having a uniform size. A spheroid-containing hydrogel, which includes a hydrogel and two or more spheroids having a uniform size with a diameter of from 70 ?m to 400 ?m that are disposed in the hydrogel in such a manner that the two or more spheroids do not contact each other, can favorably maintain the function of the plural spheroids contained within the hydrogel.Type: ApplicationFiled: September 8, 2009Publication date: January 19, 2012Applicant: TOKYO UNIVERSITY OF SCIENCE EDUCATIONAL FOUNDATION ADMINISTRATIVE ORG.Inventors: Hidenori Otsuka, Tomomi Satomi, Koji Ueno, Masashi Yamamoto, Yuichi Nakasone, Kyoko Akashi
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Patent number: 8093039Abstract: A surface coating comprises a primer coat that permits adhesion of eukaryotic cells thereto, and a plurality of macromolecular structures attached to the primer coat. At least some of the macromolecular structures have a cell-resistant character, meaning that cells generally will not adhere to them. The macromolecular structures are distributed across an area of the primer coat so that the surface coating permits adhesion of the eukaryotic cells to the primer layer and resists the adhesion of non-eukaryotic cells. Typically, the primer coat comprises a self-assembled polymeric monolayer and the macromolecular structures comprise nanoscale hydrogels. Such surface coatings may be formed on articles of manufacture for insertion into the body, such as orthopedic devices.Type: GrantFiled: April 8, 2008Date of Patent: January 10, 2012Assignee: The Trustees of the Stevens Institute of TechnologyInventor: Matthew R. Libera
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Publication number: 20110306110Abstract: A method for preparing a biomaterial comprising a gel layer which forms a core region, and cells (cover cells) which cover around the gel layer, said method comprising the steps of: (a) bringing a biocompatible oil or a vegetable oil or a mixture thereof with a mineral oil into contact with a solution containing a gel-forming component to form monodisperse droplets; (b) inducing gelation of the monodisperse droplets to give gel beads; and (c) seeding the cover cells over the surface of the gel beads.Type: ApplicationFiled: December 14, 2009Publication date: December 15, 2011Applicant: THE UNIVERSITY OF TOKYOInventors: Shoji Takeuchi, Yukiko Matsunaga, Yuya Morimoto