Involving Fixed Or Stabilized, Nonliving Microorganism, Cell, Or Tissue (e.g., Processes Of Staining, Stabilizing, Dehydrating, Etc.; Compositions Used Therefore, Etc.) Patents (Class 435/40.5)
  • Patent number: 11635355
    Abstract: A method is disclosed that permits calculation of reagent concentrations (in SI units) over time and space within a tissue sample as the sample is immersed in the reagent and the reagent diffuses into the tissue sample. The disclosed method has yielded the surprising result that once a formaldehyde concentration at all points within a tissue sample exceeds about 90 mM during a cold step of a cold+hot fixation protocol, the hot step of the fixation protocol can be commenced to provide reliable detection of molecular targets and preservation of tissue morphology in downstream analyses.
    Type: Grant
    Filed: June 13, 2019
    Date of Patent: April 25, 2023
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Daniel Bauer, David Chafin, Michael Otter
  • Patent number: 11635446
    Abstract: A holder system for a scanning probe microscope, including a holder and a fixer. The holder includes a holder top surface, and a holder sidewall. The holder top surface is configured to hold a sample. The holder sidewall extends from the holder top surface. The fixer includes a fixer fixing portion, and a fixer connecting portion. The fixer fixing portion is configured to affix the sample to the holder top surface. The fixer connecting portion is connected to the fixer fixing portion, and is affixed to the holder sidewall. An area to-be-tested of the sample is exposed to the fixer fixing portion.
    Type: Grant
    Filed: March 29, 2022
    Date of Patent: April 25, 2023
    Assignee: WINBOND ELECTRONICS CORP.
    Inventor: Hsueh-Cheng Liao
  • Patent number: 11630038
    Abstract: A method and system are described for processing tissues according to particular processing protocols that are established based on time-of-flight measurements as a processing fluid is diffused into a tissue sample. In one embodiment, measurement of the time it takes about 70% ethanol to diffuse into a tissue sample is used to predict the time it will take to diffuse other processing fluids into the same or similar tissue samples. Advantageously, the disclosed method and system can reduce overall processing times and help ensure that only samples that require similar processing conditions are batched together.
    Type: Grant
    Filed: June 13, 2019
    Date of Patent: April 18, 2023
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Daniel Bauer, Michael Otter
  • Patent number: 11610272
    Abstract: A system and method for predicting a crop yield for a type of seed in a location is described. The method includes receiving, at a client device, seasonal crop data for the type of seed, soil data associated with the location, and mapping data associated with the location. The soil data includes soil variables, and the location is represented by voxels. The seasonal crop data, the soil data and the mapping data are uploaded to a geospatial database associated with a crop prediction engine. A random forest prediction model is applied to the seasonal crop data, the soil data and mapping data in the geospatial database by the crop prediction engine, which then ranks covariates to determine one or more significant covariates. The crop prediction engine then re-applies the significant covariates to the random forest prediction model to predict the crop yield for the type of seed at the location.
    Type: Grant
    Filed: January 27, 2021
    Date of Patent: March 21, 2023
    Assignee: ARVA INTELLIGENCE CORP.
    Inventors: John A. McEntire, Thomas A. Dye
  • Patent number: 11561162
    Abstract: It is aimed to provide a technology that enables highly accurate device performance evaluation and device adjustment in optical analysis of microparticles, using the same type of beads. The present technology provides an information processing device including an information processing unit that acquires a plurality of fluorescence intensities at a plurality of light irradiation powers for a fluorescence signal from a sample including particles labeled with a fluorescent dye having a single fluorescence intensity, recognizes an intensity range of each of the plurality of fluorescence intensities detected on the basis of a fluorescence intensity balance of the sample, and calculates information relating to sensitivity of a fluorescence detection unit.
    Type: Grant
    Filed: January 28, 2019
    Date of Patent: January 24, 2023
    Assignee: SONY CORPORATION
    Inventor: Katsutoshi Tahara
  • Patent number: 11538571
    Abstract: Methods and systems for automating and managing efficient workflow for the viewing and analysis of diagnostic images within a healthcare enterprise network generate virtual worklists listing of healthcare data orders allocated to a specific destination. The methods and systems can provide flexible worklist viewing, system administration, and maximized diagnostic throughput.
    Type: Grant
    Filed: December 14, 2018
    Date of Patent: December 27, 2022
    Assignee: Compressus, Inc.
    Inventors: Albert Hernandez, Simon Liao, Raymundo Anaya, Laszlo R. Gasztonyi, Ronald G. Gesell, Bernadette Baclig, Joel Rosenfield
  • Patent number: 11513333
    Abstract: A system for characterizing at least one particle from a fluid sample is disclosed. The system includes a filter disposed upstream of an outlet, and a luminaire configured to illuminate the at least one particle at an oblique angle. An imaging device is configured to capture and process images of the illuminated at least one particle as it rests on the filter for characterizing the at least one particle. A system for characterizing at least one particle using bright field illumination is also disclosed. A method for characterizing particulates in a fluid sample using at least one of oblique angle and bright field illumination is also disclosed.
    Type: Grant
    Filed: February 21, 2017
    Date of Patent: November 29, 2022
    Assignee: Optofluidics Inc.
    Inventors: Colby Ashcroft, Brian DiPaolo, Gjergji Konica, Thomas Castner, Bernardo Cordovez, Christopher Earhart, David Erickson, Robert Hart
  • Patent number: 11506612
    Abstract: A system and method for detecting pathogenetic analytes including exciting a large target input area with radiation to produce scattered light to form an input beam, reformatting, with an optical slicer system, the input beam to produce an output beam, dispersing the output beam to produce an output area, capturing excitation data from the output area; and determining, with a processor, a presence of a particular analyte in the input area based on the excitation data. The input area can be greater than 100 micron squared and less than one million microns squared. The optical slicer system can be a high throughput virtual slit system. SERS analysis detects analytes of interest with both high resolution and sensitivity simultaneously, and is applicable for detection of the presence of viruses.
    Type: Grant
    Filed: July 29, 2021
    Date of Patent: November 22, 2022
    Assignee: TH Bioventures, LLC
    Inventors: Leif E. Honda, Phil A Cestaro, Jon C Wetzel, Songhao Li, Arsen Hajian, Ronald J Sostek
  • Patent number: 11396011
    Abstract: A device for taking and optionally processing a sample, includes (i) a housing containing a porous matrix that can receive the sample, (ii) a stopper that can be connected to the housing in a tight manner and including a piston that ensures the tight closing of the housing, compressing the porous matrix or the sample, and (iii) a storage receptacle that can be connected to the housing, can receive the sample that has passed through the porous matrix, and includes at least one conduit connecting the inside of the receptacle to the outside, once the porous matrix or the sample is compressed. The device also includes a seal between the stopper and the storage receptacle. The stopper closes the storage receptacle in a tight manner when the stopper and the storage receptacle are connected to the housing.
    Type: Grant
    Filed: October 23, 2017
    Date of Patent: July 26, 2022
    Assignee: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
    Inventor: Herve Volland
  • Patent number: 11391653
    Abstract: The present invention relates to a composition for immunostaining cleared large tissues and a method for immunostaining cleared large biological tissues using the same. The composition for immunostaining cleared large tissues increases antibody permeability in cleared biological tissues, in particular, large biological tissues having a thickness of 3 mm or more, to allow antibodies to penetrate deep into thick tissues, thereby overcoming immunostaining problems associated with limited permeation of antibodies in existing methods, reduces the time required for immunostaining large tissues, thereby enabling simple and rapid three-dimensional bioimaging with high resolution for large biological tissues, and may be beneficially used, through structure images, for identifying the causes of various diseases, developing new treatment methods, and predicting the efficacy and toxicity of drugs.
    Type: Grant
    Filed: February 14, 2019
    Date of Patent: July 19, 2022
    Assignee: Korea Research Institute of Chemical Technology
    Inventors: SunHyun Park, Ki-Suk Kim
  • Patent number: 11367294
    Abstract: An image capturing system includes a camera unit that captures an image of a cell; a display unit; an input unit that receives input from an operator regarding a selection operation on the cell; an analyzing unit that analyzes the image corresponding to the cell to which the selection operation is given and extracts a feature amount of the cell; and a specifying unit that specifies a recommended cell. The specifying unit sets a parameter that defines a range of the cell to be selected based on the feature amount of the cell to which the selection operation is given up to a first time point. In an image including the cell obtained by image capturing at a second time point later than the first time point, the specifying unit specifies the recommended cell on which the operator is prompted to make a selection based on the parameter.
    Type: Grant
    Filed: December 4, 2018
    Date of Patent: June 21, 2022
    Assignee: YAMAHA HATSUDOKI KABUSHIKI KAISHA
    Inventor: Masaru Sakamoto
  • Patent number: 11187665
    Abstract: A method determines a calibration specification specifying a functional correlation between an NMR measurement variable, determined using an NMR measuring device, for a material to be analyzed and a humidity contained in the material to be analyzed. The method includes provision of a relaxation curve of the material having a known humidity; determination of a relaxation time distribution from the relaxation curve provided; and determination of an approximate relaxation time distribution relative to at least one other humidity. The at least one other humidity is less than the known humidity.
    Type: Grant
    Filed: September 14, 2018
    Date of Patent: November 30, 2021
    Assignee: Robert Bosch GmbH
    Inventor: Markus Edelmann
  • Patent number: 11144774
    Abstract: A system and a method for unveiling poorly visible or lightly colored nuclei in an input image are disclosed. An input image is fed to a color deconvolution module for deconvolution into two color channels that are processed separately before being combined. The input image is deconvolved into two separate images: a stain image and a counter stain image. A complement of the stain image is generated in order to clearly reflect the locations of the poorly visible or light-colored nuclei. The complement image and the counter stain image are optionally normalized and then combined and segmented, to generate an output image with clearly defined nuclei. Alternatively, the complement of the stain image and the counter stain image can optionally be normalized, and then segmented prior to being combined to generate the output image.
    Type: Grant
    Filed: January 31, 2020
    Date of Patent: October 12, 2021
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Christoph Guetter, Kien Nguyen, XiuZhong Wang
  • Patent number: 11048911
    Abstract: The present invention relates to an apparatus (10) for determining cellular composition in one or more tissue sample microscopic images. It is described to provide (210) first image data of at least one tissue sample. The first image data relates to a non-specific staining of a tissue sample of the at least one tissue sample. Second image data of the at least one tissue sample is provided (220). The second image data relates to specific staining of a tissue sample of the at least one tissue sample. Either (i) the tissue sample that has undergone specific staining is the same as the tissue sample that has undergone non-specific staining, or (ii) the tissue sample that has undergone specific staining is different to the tissue sample that has undergone non-specific staining. A non-specific cellular composition cell density map is determined (230) on the basis of the first image data. A specific cellular composition cell density map is determined (240) on the basis of the second image data.
    Type: Grant
    Filed: October 26, 2017
    Date of Patent: June 29, 2021
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Christiaan Varekamp, Reinhold Wimberger-Friedl, David Halter
  • Patent number: 11016101
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: October 31, 2018
    Date of Patent: May 25, 2021
    Assignee: LITRON LABORATORIES LTD.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 10977791
    Abstract: Described herein are computer-implemented methods for analysis of a tissue sample. An example method includes: annotating the whole tumor regions or set of tumorous sub-regions either on a biomarker image or an H&E image (e.g. from an adjacent serial section of the biomarker image); registering at least a portion of the biomarker image to the H&E image; detecting different cellular and regional tissue structures within the registered H&E image; computing a probability map based on the different detected structures within the registered H&E image; deriving nuclear metrics from each of the biomarker and H&E images; deriving probability metrics from the probability map; and classifying tumor nuclei in the biomarker image based on the computed nuclear and probability metrics.
    Type: Grant
    Filed: June 21, 2019
    Date of Patent: April 13, 2021
    Assignee: VENTANA MEDICAL SYSTEMS, INC.
    Inventors: Srinivas Chukka, Kien Nguyen, Ting Chen
  • Patent number: 10889720
    Abstract: The present invention provides a nanoparticle containing a hydrophobic fluorescent substance accumulated therein and a thermosetting resin as a matrix, which nanoparticle, when used for labeling of a biological substance such as a protein or nucleic acid, has brightness sufficient for allowing pathological diagnosis using a fluorescence image obtained thereby. The present invention is a phosphor integrated dots nanoparticles wherein a thermosetting resin contains a structural unit formed from a raw material containing a hydrophobic substituent, and wherein a fluorescent substance is accumulated in the nanoparticle at least by hydrophobic interaction, preferably further by stacking interaction, with the hydrophobic substituent of the thermosetting resin.
    Type: Grant
    Filed: December 6, 2016
    Date of Patent: January 12, 2021
    Assignee: KONICA MINOLTA, INC.
    Inventors: Takuji Aimiya, Kensaku Takanashi
  • Patent number: 10859564
    Abstract: Implementations of the present invention relate to apparatuses, systems, and methods for constructing and using a metastatic mimetic device. The device includes at least one chamber with a gate structure that allows a channel to selectively allow fluid communication between an interior of the chamber and an exterior of the chamber. The channel includes a porous member extending across a cross-section of the channel to control flow rates or allow the mimetic device to replicate transport across a member.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: December 8, 2020
    Assignee: University of Kansas
    Inventors: Nikki Cheng, Wei Bin Fang, Terry N. Faddis, John Preston White, III
  • Patent number: 10846848
    Abstract: An image processing system configured to receive a first fluorescence image generically indicating the presence of biological matter; transform the first image into a transformed first image having a first color; for each of the biomarkers, receive a respective second fluorescence image indicating signals emitted by fluorescence stain selectively staining the biomarker, transforming the second images into a respective transformed second images having a respective second color; overlaying and combining the transformed first and second images; storing and/or displaying the combined image as the simulated digital bright field IHC or ISH image. The first image is created using an autofluorescence reference spectrum of the tissue sample or of a similar tissue sample or by using a fluorescence reference spectrum of a first stain which generically binds to biological matter of the tissue sample for spectrally unmixing of a multi-spectral digital image of the tissue sample.
    Type: Grant
    Filed: June 9, 2017
    Date of Patent: November 24, 2020
    Assignee: Hoffman-La Roche Inc.
    Inventor: Eldad Klaiman
  • Patent number: 10830770
    Abstract: The present invention relates to a method for detecting cell death using a luminescent compound; to the luminescent compounds for particular uses; to a kit comprising said compounds and to a protein. The method is applicable for detecting cell death, essentially regardless of the mechanism through which cell death occurred or is occurring and is therefore not limited e.g. to detecting cell death resulting from only one mechanism selected from apoptosis and necrosis.
    Type: Grant
    Filed: February 6, 2013
    Date of Patent: November 10, 2020
    Inventors: Markwin Hendrik Maring, Clemens Waltherus Gerardus Löwik, Ermond Reijer Van Beek
  • Patent number: 10834308
    Abstract: An imaging system may include a sample stage having a surface to support a sample to be scanned by the imaging system; an optical stage having an objective lens, the optical stage being positionable relative to the sample stage; an actuator physically coupled to at least one of the sample stage and the optical stage to move the sample stage relative to the optical stage; a servo circuit to control the actuator; a first set of control parameters to control the servo circuit; a second set of control parameters to control the servo circuit; and a servo control circuit to apply the first set of control parameters to the servo circuit when the imaging system is operating in a first mode of operation and to apply the second set of control parameters to the servo circuit when the imaging system is operating in a second mode of operation.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: November 10, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Chunhong (Allen) Zhou, John Bailey, Dustin Blair
  • Patent number: 10802013
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: October 13, 2020
    Assignee: LITRON LABORATORIES LTD.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 10786188
    Abstract: A method for in-vivo cancer diagnosis within a living tissue. The method includes preparing an electrochemical probe by fabricating three integrated electrodes via coating a layer of vertically aligned multi-walled carbon nanotubes (VAMWCNTs) on tips of three electrically conductive biocompatible needles, putting the tips of the three integrated electrodes in contact with a portion of the living tissue by inserting the tips of the three integrated electrodes into the portion of the living tissue, recording an electrochemical response from the portion of the living tissue, where the electrochemical response may include a cyclic voltammetry (CV) diagram with an oxidation current peak corresponding to a hypoxic glycolysis chemical reaction in biological cells within the portion of the living tissue, and detecting a cancer-involving status of the portion of the living tissue based on the oxidation current peak.
    Type: Grant
    Filed: April 24, 2020
    Date of Patent: September 29, 2020
    Inventors: Mohammad Abdolahad, Zohreh Sadat Miripour, Sahar NajafiKhoshnoo
  • Patent number: 10752955
    Abstract: Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: August 25, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Michael Farrell, Antony Hubbard, Donald Johnson, Brian Kelly, Taylor Shingler, Lei Tang, Wenjun Zhang
  • Patent number: 10747986
    Abstract: Systems and methods for automated analysis of histochemical or cytological samples stained for analytes having complex staining patterns are presented. Analyte staining of a biological compartment in a region is quantified, in which said staining is intermixed with analyte staining of an analytically-distinct biological compartment. The systems and methods further include identifying membrane staining of an analyte of interest in regions where diffuse membrane staining is intermixed with cytoplasmic staining and/or punctate staining is disclosed. The systems and methods further include quantifying membrane staining of an analyte of interest in tissue or cytological samples having regions in which membrane staining is intermixed with cytoplasmic staining and/or punctate staining.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: August 18, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Christophe Chefd'hotel, Kien Nguyen
  • Patent number: 10702549
    Abstract: The present invention provides a stabilized hypohalous acid solution (or formulation thereof), which may be conveniently packaged for sale, or stored for later use on demand. The invention further provides methods of making the stabilized hypohalous acid solutions, as well as methods of use for disinfecting mammalian tissue, including wounds and burns, and treating skin irritations, among other uses.
    Type: Grant
    Filed: October 29, 2018
    Date of Patent: July 7, 2020
    Assignee: URGO US, INC.
    Inventors: Mark Sampson, Svetlana Panicheva, Cary Schockemoehl, Gerard Dibello, Vadim Panichev
  • Patent number: 10678040
    Abstract: A microscope slide mounting system includes a slide mounting medium, with a predetermined weight of powdered gum arabic in a predetermined weight of distilled water; a predetermined weight of anhydrous chloral; a predetermined weight of anhydrous glycerin; and a predetermined percentage of ethylene glycol; the ethylene glycol acts as a denaturant in the mounting medium; and the ethylene glycol causes mild illness when ingested, thereby discouraging the use of the mounting medium as a sedative or recreational drug and prevents the crystallization of the medium.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: June 9, 2020
    Inventor: Thomas Edward Hays
  • Patent number: 10663377
    Abstract: A clearing agent and mounting solution for microscopy is disclosed comprising at least trichloroethanol and/or derivatives thereof, where the refractive index of the solution is greater than or equal to about 1.3810. Also disclosed are methods of preparing specimens for microscopy.
    Type: Grant
    Filed: July 9, 2018
    Date of Patent: May 26, 2020
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: James E. Simon, Thomas Villani, Adolfina Koroch
  • Patent number: 10621307
    Abstract: A system for generating image-based patient profiles acquires digital images from tissue samples and CT and MRI scans. The system detects objects within the images, measures values related to the detected objects, and displays the measured values in patient profile lists that indicate the normal ranges for measured values. The system indicates which measured values fall outside the normal ranges and navigates the user to the objects in the images associated with the abnormal values when the user selects a measured value in the patient profile. Various risks of the existence of different diseases and the probability of success of specific treatments are displayed on a graphical user interface. The system searches for patterns in the patient data and profile lists that reflect those specific risks and success probabilities. A high probability of disease risk or of the success of a specific treatment is indicated on the graphical user interface.
    Type: Grant
    Filed: April 29, 2010
    Date of Patent: April 14, 2020
    Assignee: Definiens GmbH
    Inventors: Gerd Binnig, Guenter Schmidt, Markus Rinecker
  • Patent number: 10620213
    Abstract: Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: April 14, 2020
    Assignee: Inguran, LLC
    Inventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Richard W. Lenz, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
  • Patent number: 10613005
    Abstract: Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: April 7, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Victor Lim, Carol T. Schembri, Adrienne Mccampbell, Nazumi Alice Yama-Dang
  • Patent number: 10605707
    Abstract: A method of removing a floatation liquid from between a microscope slide and a paraffin embedded biological specimen including position the microscope slide with the paraffin embedded biological specimen floated thereon onto a slide support element. The slide support element is rotated to cause the microscope slide and the paraffin embedded biological specimen to move in a way that causes the floatation liquid disposed between the microscope slide and the paraffin embedded biological specimen to be removed from between the microscope slide and the paraffin embedded biological specimen.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: March 31, 2020
    Inventor: Lee H. Angros
  • Patent number: 10453195
    Abstract: A tissue area detection method in digital pathology imaging uses an automated method to detect tissue area of interest (AOI detection) on a Whole Slide Analysis (WSA) or Tissue Micro Array (TMA) thumbnail image. The present method may use preprocessing of the image followed by a two-pass segmentation technique for separating tissue areas from non-tissue areas. The present method may further use global and local window statistics for thresholding to overcome variations in staining intensity that may hamper accurate selection of the area of interest. The present method may also have a classifying process of the tissue area based on the staining method used and applying stain specific filters to remove unwanted artifacts.
    Type: Grant
    Filed: August 18, 2017
    Date of Patent: October 22, 2019
    Assignee: OptraScan, Inc.
    Inventors: Abhijeet Gholap, Anagha Jadhav, Isha Doshi, Gurunath Kamble
  • Patent number: 10429304
    Abstract: This invention relates to enrichment of a biological sample comprising a plurality of cells to assist further analysis thereof. It provides a technique comprising the steps of: (a) providing a sample comprising a plurality of cells which include a photosensitive compound that can be induced by light irradiation to inactivate or kill at least part of the respective cell; (b) acquiring an image of at least a portion of the sample; (c) identifying cells of interest in the sample image; (d) selecting cells other than the cells identified in step (c); and (e) irradiating only those cells selected in step (d) with a light beam so as induce the photosensitive compound therein to inactivate or kill at least part of those cells, and thereby enrich the sample with respect to the cells of interest for further analysis.
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: October 1, 2019
    Assignee: PerkinElmer Singapore PTE Ltd.
    Inventors: Patrick Courtney, Alistair Fitch
  • Patent number: 10376250
    Abstract: A medical navigation system is provided for performing at least part of an assessment of a non-living body. The medical navigation system comprises a positioning device having a positioning arm with an end effector at the end of the positioning arm, an imaging device coupled to the end effector, and a controller electrically coupled to the positioning device and the imaging device. The controller has a processor coupled to a memory and a display. The controller is configured to generate a signal to move the positioning arm to position the imaging device through a range of motion to perform a scan of a surface of the body and receive and save as data in the memory signals generated by the imaging device during the range of motion.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: August 13, 2019
    Assignee: SYNAPTIVE MEDICAL (BARBADOS) INC.
    Inventors: Kai Michael Hynna, Joshua Lee Richmond, Alexander Gyles Panther, Thanh Vinh Vuong
  • Patent number: 10368541
    Abstract: A preservative reagent for urine is disclosed that increases the stability of cells, such as tumor cells, in urine for a period of several weeks. The preservative reagent comprises polyethylene glycol (PEG), ethanol, paraformaldehyde (PFA), and ethylenediaminetetraacetic acid (EDTA), and optionally pH stabilizing reagents.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: August 6, 2019
    Assignee: CREATV MICROTECH, INC.
    Inventors: Peixuan Zhu, Daniel Adams, Cha-Mei Tang
  • Patent number: 10330680
    Abstract: Methods of isolating and assaying fetal extravillous trophoblast cells, including assays of RNA of the fetal extravillous trophoblast cells according to aspects of the disclosure include obtaining a maternal endocervical sample containing fetal extravillous trophoblast cells from a pregnant subject; fixing the maternal endocervical sample in an aldehyde fixative, removing fetal extravillous trophoblast cells from the maternal endocervical sample thereby producing isolated extravillous trophoblast cells, isolating and assaying RNA from the fetal extravillous trophoblast cells.
    Type: Grant
    Filed: October 12, 2015
    Date of Patent: June 25, 2019
    Assignee: Wayne State University
    Inventors: D. Randall Armant, Sascha Drewlo
  • Patent number: 10209192
    Abstract: A system and method for detecting glycation levels within hemoglobin generates a first correlation matrix using a first light beam at a first wavelength passing through a hemoglobin sample. A second correlation matrix is generated using a second light beam at a second wavelength passing through the hemoglobin sample. The first correlation matrix is multiplied by an inverse of the second correlation matrix to obtain a third matrix. A fourth matrix is generated by taking a singular value decomposition of the third matrix. The fourth matrix comprises a unique biomarker for a level of glycation within the hemoglobin sample. The level of glycation within the hemoglobin sample is determined responsive to the fourth matrix and an indicator identifying the determined level of glycation is output.
    Type: Grant
    Filed: June 12, 2018
    Date of Patent: February 19, 2019
    Assignee: NXGEN PARTNERS IP, LLC
    Inventor: Solyman Ashrafi
  • Patent number: 10139335
    Abstract: An observation image determination device includes an observation image acquisition unit that captures an image of an observation region including a stem cell to be cultured to acquire an observation image and a determination unit that determines whether a living body of a different type from the stem cell is included in the observation region. The determination unit determines whether the different type of living body is included in the observation region, on the basis of at least one of form information of an observation target and information about a change in the observation target over time which are acquired from the observation image.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: November 27, 2018
    Assignee: FUJIFILM Corporation
    Inventor: Kenta Matsubara
  • Patent number: 10126216
    Abstract: An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: November 13, 2018
    Assignee: Ventana Medical Systems, Inc.
    Inventors: David Chafin, Abbey Pierson Theiss, Michael Otter, Esteban Roberts
  • Patent number: 9964473
    Abstract: A method of removing a floatation liquid from between a microscope slide and a paraffin embedded biological specimen including position the microscope slide with the paraffin embedded biological specimen floated thereon onto a slide support element. The slide support element is rotated to cause the microscope slide and the paraffin embedded biological specimen to move in a way that causes the floatation liquid disposed between the microscope slide and the paraffin embedded biological specimen to be removed from between the microscope slide and the paraffin embedded biological specimen.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: May 8, 2018
    Inventor: Lee H. Angros
  • Patent number: 9946953
    Abstract: A computer implemented image processing method is provided for identifying a tissue boundary of a tumor region of a tissue sample, the tissue sample containing non-tumor regions and at least one tumor region. The method includes obtaining an image of a tissue section of the tissue sample, identifying at least one image property of the image, and comparing the image property with classification data. The method further includes, based on the comparison, classifying a region of the image as a tumor region representing a tumor region in the tissue sample or a non-tumor region representing a non-tumor region in the tissue sample. If the region of the image is classified as a tumor region, the method includes identifying a boundary of the region of the image, and using the boundary to identify a tissue boundary of the tumor region of the tissue sample represented by the region of the image.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: April 17, 2018
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Jonathon Tunstall, Peter Hamilton, Yinhai Wang, David McCleary, James Diamond
  • Patent number: 9857358
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: September 12, 2016
    Date of Patent: January 2, 2018
    Assignee: Litron Laboratories Ltd.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 9714948
    Abstract: The invention provides a method for detecting one or more biological ligands, where the method generally uses two arrays of biological reagents. The two arrays have two different functionalities: the first array captures the ligands on the array; and the second array delivers detecting reagents to the captured ligands. Use of arrays affords the method high-throughput detection capability; and the use of two different arrays ensures high specificity. The first array has a support structure fixed with a first set of reagents, each at a pre-determined position on the support. The second array has a support structure fixed with a second set of reagents, each at a pre-determined position on the support. The first array contains reagent(s) binding to a ligand, while the second array contains second reagent(s) binding to the same ligand. In preferred embodiments, antibodies are used as reagents, and used in detecting proteins in protein samples.
    Type: Grant
    Filed: September 8, 2015
    Date of Patent: July 25, 2017
    Inventor: Yingjian Wang
  • Patent number: 9687567
    Abstract: The present invention relates to compositions for and methods of optically imaging tissues or cells using imaging agents having desirable in vivo properties that result in improved signal-to-background ratio.
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: June 27, 2017
    Assignees: Beth Israel Deaconess Medical Center, Inc., Georgia State University Research Foundation Inc.
    Inventors: John V. Frangioni, Maged M. Henary
  • Patent number: 9649169
    Abstract: The invention concerns an analysis method for a surgical operation via endoscopy performed at an operative site in a volume located inside the body of an animal, characterized in that it comprises the steps consisting of: detecting at least one instrument (1; 2; 3) present at the operative site, detecting at least one portion of an organ (4) in the vicinity of the detected instrument (1; 2; 3), determining the positioning of the detected instrument (1; 2; 3) relative to the detected organ (4), so that the detected instrument (1; 2; 3), the detected portion of organ (4) and their relative positioning form parameters defining a detected surgical step, selecting, in a database, a conceptual surgical step corresponding to the detected surgical step from to the detected instrument (1; 2; 3), the detected portion of organ (4) and their relative positioning, comparing the parameters of the detected surgical step with the parameters defining the selected conceptual surgical step to determine the differences betwee
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: May 16, 2017
    Assignee: Universite Grenoble Alpes
    Inventors: Philippe Cinquin, Sandrine Voros, Pierre Mozer, Aurel Messas, Jean-Alexandre Long, Alexandre Moreau-Gaudry
  • Patent number: 9523629
    Abstract: A method and apparatus are provided for forming high-yield tissue microarray blocks capable of producing 1,000 or more replicate slides. In one embodiment, a plurality of donor tissue samples are minced and suspended in separate molten liquid carriers to form a plurality of separate two-phase mixtures of solid minced tissue and molten wax. Each of the two-phase mixtures is preferably formed into elongated columns and transferred into a separate well of a microarray block. A method and apparatus are provided for forming the elongated molten wax columns with minced tissue evenly distributed along the length of each column. The apparatus for forming the array includes an extrusion mechanism for transferring the columns of two-phase mixtures into the deep wells of the microarray block. The two-phase mixture may alternately be cooled and allowed to solidify before being transferred into the microarray block.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: December 20, 2016
    Assignee: Array Sciences, LLC
    Inventors: Regan Spencer Fulton, William Scott Crawford
  • Patent number: 9506928
    Abstract: Provided herein are methods, compounds, mixtures and formulations of antigen retrieval agent useful in retrieving antigens and improving the detection of amino acids, peptides and proteins or epitopes thereon in a tissue fixed with aldehyde-based cross-linking agents. Contacting the fixed tissue with a solution of the aldehyde scavenging agent causes reactivity with the aldehyde moieties to retrieve antigens and improve detection of the amino acids, peptides and proteins or epitopes. Also provided are kits comprising the antigen retrieval agent and, optionally, components for staining or detecting the proteins or the antigens or epitopes and instructions for using the kit. Further provided is a method for identifying an antigen retrieval agent. A fixed protein is contacted with an agent to be tested and heated in solution therewith. Detection of protein peaks via mass spectrometry indicates the tested agent is an antigen retrieval agent.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: November 29, 2016
    Assignee: UNIVERSITY OF HOUSTON SYSTEM
    Inventors: Jason Eriksen, Craig Vollert, Steven Bark, Wilna Moree
  • Patent number: 9470694
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: October 18, 2016
    Assignee: Litron Laboratories Ltd.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 9429520
    Abstract: The present invention relates to enrichment and/or identification of fetal cells of a maternal blood sample using fetal cell specific ligands and/or fetal cell specific hybridization probes wherein the ligand or probes are directed to an endothelial/mesenchymal marker, e.g. CD105, CD146 or CD141, in a first round of enrichment and the ligand or probes, in a second round of enrichment, are directed to an epithelial marker, e.g. a cytokeratin, such as CK7, CK8, CK18 or CK19. Enriched or identified fetal cells may be subjected to steps of detection or diagnosis, wherefore the present invention enables non-invasive 5 prenatal diagnostics.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: August 30, 2016
    Assignee: Arcedi Biotech ApS
    Inventors: Andreas Eckelt, Britta Christensen, Steen Kolvraa, Marie Brinch, Ripudaman Singh, Lotte Hatt