Involving Fixed Or Stabilized, Nonliving Microorganism, Cell, Or Tissue (e.g., Processes Of Staining, Stabilizing, Dehydrating, Etc.; Compositions Used Therefore, Etc.) Patents (Class 435/40.5)
  • Patent number: 10889720
    Abstract: The present invention provides a nanoparticle containing a hydrophobic fluorescent substance accumulated therein and a thermosetting resin as a matrix, which nanoparticle, when used for labeling of a biological substance such as a protein or nucleic acid, has brightness sufficient for allowing pathological diagnosis using a fluorescence image obtained thereby. The present invention is a phosphor integrated dots nanoparticles wherein a thermosetting resin contains a structural unit formed from a raw material containing a hydrophobic substituent, and wherein a fluorescent substance is accumulated in the nanoparticle at least by hydrophobic interaction, preferably further by stacking interaction, with the hydrophobic substituent of the thermosetting resin.
    Type: Grant
    Filed: December 6, 2016
    Date of Patent: January 12, 2021
    Assignee: KONICA MINOLTA, INC.
    Inventors: Takuji Aimiya, Kensaku Takanashi
  • Patent number: 10859564
    Abstract: Implementations of the present invention relate to apparatuses, systems, and methods for constructing and using a metastatic mimetic device. The device includes at least one chamber with a gate structure that allows a channel to selectively allow fluid communication between an interior of the chamber and an exterior of the chamber. The channel includes a porous member extending across a cross-section of the channel to control flow rates or allow the mimetic device to replicate transport across a member.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: December 8, 2020
    Assignee: University of Kansas
    Inventors: Nikki Cheng, Wei Bin Fang, Terry N. Faddis, John Preston White, III
  • Patent number: 10846848
    Abstract: An image processing system configured to receive a first fluorescence image generically indicating the presence of biological matter; transform the first image into a transformed first image having a first color; for each of the biomarkers, receive a respective second fluorescence image indicating signals emitted by fluorescence stain selectively staining the biomarker, transforming the second images into a respective transformed second images having a respective second color; overlaying and combining the transformed first and second images; storing and/or displaying the combined image as the simulated digital bright field IHC or ISH image. The first image is created using an autofluorescence reference spectrum of the tissue sample or of a similar tissue sample or by using a fluorescence reference spectrum of a first stain which generically binds to biological matter of the tissue sample for spectrally unmixing of a multi-spectral digital image of the tissue sample.
    Type: Grant
    Filed: June 9, 2017
    Date of Patent: November 24, 2020
    Assignee: Hoffman-La Roche Inc.
    Inventor: Eldad Klaiman
  • Patent number: 10834308
    Abstract: An imaging system may include a sample stage having a surface to support a sample to be scanned by the imaging system; an optical stage having an objective lens, the optical stage being positionable relative to the sample stage; an actuator physically coupled to at least one of the sample stage and the optical stage to move the sample stage relative to the optical stage; a servo circuit to control the actuator; a first set of control parameters to control the servo circuit; a second set of control parameters to control the servo circuit; and a servo control circuit to apply the first set of control parameters to the servo circuit when the imaging system is operating in a first mode of operation and to apply the second set of control parameters to the servo circuit when the imaging system is operating in a second mode of operation.
    Type: Grant
    Filed: January 10, 2019
    Date of Patent: November 10, 2020
    Assignee: ILLUMINA, INC.
    Inventors: Chunhong (Allen) Zhou, John Bailey, Dustin Blair
  • Patent number: 10830770
    Abstract: The present invention relates to a method for detecting cell death using a luminescent compound; to the luminescent compounds for particular uses; to a kit comprising said compounds and to a protein. The method is applicable for detecting cell death, essentially regardless of the mechanism through which cell death occurred or is occurring and is therefore not limited e.g. to detecting cell death resulting from only one mechanism selected from apoptosis and necrosis.
    Type: Grant
    Filed: February 6, 2013
    Date of Patent: November 10, 2020
    Inventors: Markwin Hendrik Maring, Clemens Waltherus Gerardus Löwik, Ermond Reijer Van Beek
  • Patent number: 10802013
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: October 13, 2020
    Assignee: LITRON LABORATORIES LTD.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 10786188
    Abstract: A method for in-vivo cancer diagnosis within a living tissue. The method includes preparing an electrochemical probe by fabricating three integrated electrodes via coating a layer of vertically aligned multi-walled carbon nanotubes (VAMWCNTs) on tips of three electrically conductive biocompatible needles, putting the tips of the three integrated electrodes in contact with a portion of the living tissue by inserting the tips of the three integrated electrodes into the portion of the living tissue, recording an electrochemical response from the portion of the living tissue, where the electrochemical response may include a cyclic voltammetry (CV) diagram with an oxidation current peak corresponding to a hypoxic glycolysis chemical reaction in biological cells within the portion of the living tissue, and detecting a cancer-involving status of the portion of the living tissue based on the oxidation current peak.
    Type: Grant
    Filed: April 24, 2020
    Date of Patent: September 29, 2020
    Inventors: Mohammad Abdolahad, Zohreh Sadat Miripour, Sahar NajafiKhoshnoo
  • Patent number: 10752955
    Abstract: Single-stranded oligonucleotide probes, systems, kits and methods for chromosome enumeration, gene copy enumeration, or tissue diagnostics. The probes are particularly suited for detecting gene amplification, deletion, or rearrangement in tissue samples in a single, dual, or multiplexed assay. The probes exhibit improved performance compared to industry leading dual-stranded probes; particularly in terms of the rate of hybridization.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: August 25, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Michael Farrell, Antony Hubbard, Donald Johnson, Brian Kelly, Taylor Shingler, Lei Tang, Wenjun Zhang
  • Patent number: 10747986
    Abstract: Systems and methods for automated analysis of histochemical or cytological samples stained for analytes having complex staining patterns are presented. Analyte staining of a biological compartment in a region is quantified, in which said staining is intermixed with analyte staining of an analytically-distinct biological compartment. The systems and methods further include identifying membrane staining of an analyte of interest in regions where diffuse membrane staining is intermixed with cytoplasmic staining and/or punctate staining is disclosed. The systems and methods further include quantifying membrane staining of an analyte of interest in tissue or cytological samples having regions in which membrane staining is intermixed with cytoplasmic staining and/or punctate staining.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: August 18, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Christophe Chefd'hotel, Kien Nguyen
  • Patent number: 10702549
    Abstract: The present invention provides a stabilized hypohalous acid solution (or formulation thereof), which may be conveniently packaged for sale, or stored for later use on demand. The invention further provides methods of making the stabilized hypohalous acid solutions, as well as methods of use for disinfecting mammalian tissue, including wounds and burns, and treating skin irritations, among other uses.
    Type: Grant
    Filed: October 29, 2018
    Date of Patent: July 7, 2020
    Assignee: URGO US, INC.
    Inventors: Mark Sampson, Svetlana Panicheva, Cary Schockemoehl, Gerard Dibello, Vadim Panichev
  • Patent number: 10678040
    Abstract: A microscope slide mounting system includes a slide mounting medium, with a predetermined weight of powdered gum arabic in a predetermined weight of distilled water; a predetermined weight of anhydrous chloral; a predetermined weight of anhydrous glycerin; and a predetermined percentage of ethylene glycol; the ethylene glycol acts as a denaturant in the mounting medium; and the ethylene glycol causes mild illness when ingested, thereby discouraging the use of the mounting medium as a sedative or recreational drug and prevents the crystallization of the medium.
    Type: Grant
    Filed: December 22, 2017
    Date of Patent: June 9, 2020
    Inventor: Thomas Edward Hays
  • Patent number: 10663377
    Abstract: A clearing agent and mounting solution for microscopy is disclosed comprising at least trichloroethanol and/or derivatives thereof, where the refractive index of the solution is greater than or equal to about 1.3810. Also disclosed are methods of preparing specimens for microscopy.
    Type: Grant
    Filed: July 9, 2018
    Date of Patent: May 26, 2020
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: James E. Simon, Thomas Villani, Adolfina Koroch
  • Patent number: 10620213
    Abstract: Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.
    Type: Grant
    Filed: October 3, 2013
    Date of Patent: April 14, 2020
    Assignee: Inguran, LLC
    Inventors: Kenneth Michael Evans, Thomas Boyd Gilligan, Richard W. Lenz, Ramakrishnan Vishwanath, Clara Gonzalez-Marin
  • Patent number: 10621307
    Abstract: A system for generating image-based patient profiles acquires digital images from tissue samples and CT and MRI scans. The system detects objects within the images, measures values related to the detected objects, and displays the measured values in patient profile lists that indicate the normal ranges for measured values. The system indicates which measured values fall outside the normal ranges and navigates the user to the objects in the images associated with the abnormal values when the user selects a measured value in the patient profile. Various risks of the existence of different diseases and the probability of success of specific treatments are displayed on a graphical user interface. The system searches for patterns in the patient data and profile lists that reflect those specific risks and success probabilities. A high probability of disease risk or of the success of a specific treatment is indicated on the graphical user interface.
    Type: Grant
    Filed: April 29, 2010
    Date of Patent: April 14, 2020
    Assignee: Definiens GmbH
    Inventors: Gerd Binnig, Guenter Schmidt, Markus Rinecker
  • Patent number: 10613005
    Abstract: Paraffin-embedded tissue, which may be disposed on a solid substrate, is prepared by a dry technique that removes paraffin from tissue without adding any liquid to the tissue, thereby rendering the tissue substantially free of paraffin. The dry technique may entail applying heat energy to the tissue effective to melt the paraffin and thereby render it flowable, and applying an electric field. The electric field is effective to impart electrical charge to the paraffin and to move the paraffin out from the tissue due to electrical charge repulsion or attraction, which may be assisted by moving an electrode utilized to generate the electric field relative to the paraffin. The electric field, or both the electric field and the heat energy, may be applied until the tissue is substantially free of paraffin.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: April 7, 2020
    Assignee: AGILENT TECHNOLOGIES, INC.
    Inventors: Victor Lim, Carol T. Schembri, Adrienne Mccampbell, Nazumi Alice Yama-Dang
  • Patent number: 10605707
    Abstract: A method of removing a floatation liquid from between a microscope slide and a paraffin embedded biological specimen including position the microscope slide with the paraffin embedded biological specimen floated thereon onto a slide support element. The slide support element is rotated to cause the microscope slide and the paraffin embedded biological specimen to move in a way that causes the floatation liquid disposed between the microscope slide and the paraffin embedded biological specimen to be removed from between the microscope slide and the paraffin embedded biological specimen.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: March 31, 2020
    Inventor: Lee H. Angros
  • Patent number: 10453195
    Abstract: A tissue area detection method in digital pathology imaging uses an automated method to detect tissue area of interest (AOI detection) on a Whole Slide Analysis (WSA) or Tissue Micro Array (TMA) thumbnail image. The present method may use preprocessing of the image followed by a two-pass segmentation technique for separating tissue areas from non-tissue areas. The present method may further use global and local window statistics for thresholding to overcome variations in staining intensity that may hamper accurate selection of the area of interest. The present method may also have a classifying process of the tissue area based on the staining method used and applying stain specific filters to remove unwanted artifacts.
    Type: Grant
    Filed: August 18, 2017
    Date of Patent: October 22, 2019
    Assignee: OptraScan, Inc.
    Inventors: Abhijeet Gholap, Anagha Jadhav, Isha Doshi, Gurunath Kamble
  • Patent number: 10429304
    Abstract: This invention relates to enrichment of a biological sample comprising a plurality of cells to assist further analysis thereof. It provides a technique comprising the steps of: (a) providing a sample comprising a plurality of cells which include a photosensitive compound that can be induced by light irradiation to inactivate or kill at least part of the respective cell; (b) acquiring an image of at least a portion of the sample; (c) identifying cells of interest in the sample image; (d) selecting cells other than the cells identified in step (c); and (e) irradiating only those cells selected in step (d) with a light beam so as induce the photosensitive compound therein to inactivate or kill at least part of those cells, and thereby enrich the sample with respect to the cells of interest for further analysis.
    Type: Grant
    Filed: February 28, 2007
    Date of Patent: October 1, 2019
    Assignee: PerkinElmer Singapore PTE Ltd.
    Inventors: Patrick Courtney, Alistair Fitch
  • Patent number: 10376250
    Abstract: A medical navigation system is provided for performing at least part of an assessment of a non-living body. The medical navigation system comprises a positioning device having a positioning arm with an end effector at the end of the positioning arm, an imaging device coupled to the end effector, and a controller electrically coupled to the positioning device and the imaging device. The controller has a processor coupled to a memory and a display. The controller is configured to generate a signal to move the positioning arm to position the imaging device through a range of motion to perform a scan of a surface of the body and receive and save as data in the memory signals generated by the imaging device during the range of motion.
    Type: Grant
    Filed: March 23, 2015
    Date of Patent: August 13, 2019
    Assignee: SYNAPTIVE MEDICAL (BARBADOS) INC.
    Inventors: Kai Michael Hynna, Joshua Lee Richmond, Alexander Gyles Panther, Thanh Vinh Vuong
  • Patent number: 10368541
    Abstract: A preservative reagent for urine is disclosed that increases the stability of cells, such as tumor cells, in urine for a period of several weeks. The preservative reagent comprises polyethylene glycol (PEG), ethanol, paraformaldehyde (PFA), and ethylenediaminetetraacetic acid (EDTA), and optionally pH stabilizing reagents.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: August 6, 2019
    Assignee: CREATV MICROTECH, INC.
    Inventors: Peixuan Zhu, Daniel Adams, Cha-Mei Tang
  • Patent number: 10330680
    Abstract: Methods of isolating and assaying fetal extravillous trophoblast cells, including assays of RNA of the fetal extravillous trophoblast cells according to aspects of the disclosure include obtaining a maternal endocervical sample containing fetal extravillous trophoblast cells from a pregnant subject; fixing the maternal endocervical sample in an aldehyde fixative, removing fetal extravillous trophoblast cells from the maternal endocervical sample thereby producing isolated extravillous trophoblast cells, isolating and assaying RNA from the fetal extravillous trophoblast cells.
    Type: Grant
    Filed: October 12, 2015
    Date of Patent: June 25, 2019
    Assignee: Wayne State University
    Inventors: D. Randall Armant, Sascha Drewlo
  • Patent number: 10209192
    Abstract: A system and method for detecting glycation levels within hemoglobin generates a first correlation matrix using a first light beam at a first wavelength passing through a hemoglobin sample. A second correlation matrix is generated using a second light beam at a second wavelength passing through the hemoglobin sample. The first correlation matrix is multiplied by an inverse of the second correlation matrix to obtain a third matrix. A fourth matrix is generated by taking a singular value decomposition of the third matrix. The fourth matrix comprises a unique biomarker for a level of glycation within the hemoglobin sample. The level of glycation within the hemoglobin sample is determined responsive to the fourth matrix and an indicator identifying the determined level of glycation is output.
    Type: Grant
    Filed: June 12, 2018
    Date of Patent: February 19, 2019
    Assignee: NXGEN PARTNERS IP, LLC
    Inventor: Solyman Ashrafi
  • Patent number: 10139335
    Abstract: An observation image determination device includes an observation image acquisition unit that captures an image of an observation region including a stem cell to be cultured to acquire an observation image and a determination unit that determines whether a living body of a different type from the stem cell is included in the observation region. The determination unit determines whether the different type of living body is included in the observation region, on the basis of at least one of form information of an observation target and information about a change in the observation target over time which are acquired from the observation image.
    Type: Grant
    Filed: February 17, 2016
    Date of Patent: November 27, 2018
    Assignee: FUJIFILM Corporation
    Inventor: Kenta Matsubara
  • Patent number: 10126216
    Abstract: An aldehyde fixative solution at a first temperature is caused to contact a tissue sample for a first time period, additionally an aldehyde fixative solution is caused to contact the tissue sample at a second temperature higher than the first temperature for a second time period. The first time period typically ranges from about 15 minutes up to about 4 hours, and the first temperature typically is from greater than 0° C. to at least 15° C. The second temperature typically is from greater than about 22° C. to about 55° C., and the second time period ranges from about 1 hour to about 4 hours. Using this process, improved tissue morphology and IHC staining as well as superior preservation of post-translation modification signals have been accomplished in approximately 4 hours compared to 24 hours for room temperature protocols, and more even morphology and antigen preservation are observed.
    Type: Grant
    Filed: February 13, 2012
    Date of Patent: November 13, 2018
    Assignee: Ventana Medical Systems, Inc.
    Inventors: David Chafin, Abbey Pierson Theiss, Michael Otter, Esteban Roberts
  • Patent number: 9964473
    Abstract: A method of removing a floatation liquid from between a microscope slide and a paraffin embedded biological specimen including position the microscope slide with the paraffin embedded biological specimen floated thereon onto a slide support element. The slide support element is rotated to cause the microscope slide and the paraffin embedded biological specimen to move in a way that causes the floatation liquid disposed between the microscope slide and the paraffin embedded biological specimen to be removed from between the microscope slide and the paraffin embedded biological specimen.
    Type: Grant
    Filed: February 6, 2017
    Date of Patent: May 8, 2018
    Inventor: Lee H. Angros
  • Patent number: 9946953
    Abstract: A computer implemented image processing method is provided for identifying a tissue boundary of a tumor region of a tissue sample, the tissue sample containing non-tumor regions and at least one tumor region. The method includes obtaining an image of a tissue section of the tissue sample, identifying at least one image property of the image, and comparing the image property with classification data. The method further includes, based on the comparison, classifying a region of the image as a tumor region representing a tumor region in the tissue sample or a non-tumor region representing a non-tumor region in the tissue sample. If the region of the image is classified as a tumor region, the method includes identifying a boundary of the region of the image, and using the boundary to identify a tissue boundary of the tumor region of the tissue sample represented by the region of the image.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: April 17, 2018
    Assignee: KONINKLIJKE PHILIPS N.V.
    Inventors: Jonathon Tunstall, Peter Hamilton, Yinhai Wang, David McCleary, James Diamond
  • Patent number: 9857358
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: September 12, 2016
    Date of Patent: January 2, 2018
    Assignee: Litron Laboratories Ltd.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 9714948
    Abstract: The invention provides a method for detecting one or more biological ligands, where the method generally uses two arrays of biological reagents. The two arrays have two different functionalities: the first array captures the ligands on the array; and the second array delivers detecting reagents to the captured ligands. Use of arrays affords the method high-throughput detection capability; and the use of two different arrays ensures high specificity. The first array has a support structure fixed with a first set of reagents, each at a pre-determined position on the support. The second array has a support structure fixed with a second set of reagents, each at a pre-determined position on the support. The first array contains reagent(s) binding to a ligand, while the second array contains second reagent(s) binding to the same ligand. In preferred embodiments, antibodies are used as reagents, and used in detecting proteins in protein samples.
    Type: Grant
    Filed: September 8, 2015
    Date of Patent: July 25, 2017
    Inventor: Yingjian Wang
  • Patent number: 9687567
    Abstract: The present invention relates to compositions for and methods of optically imaging tissues or cells using imaging agents having desirable in vivo properties that result in improved signal-to-background ratio.
    Type: Grant
    Filed: April 7, 2015
    Date of Patent: June 27, 2017
    Assignees: Beth Israel Deaconess Medical Center, Inc., Georgia State University Research Foundation Inc.
    Inventors: John V. Frangioni, Maged M. Henary
  • Patent number: 9649169
    Abstract: The invention concerns an analysis method for a surgical operation via endoscopy performed at an operative site in a volume located inside the body of an animal, characterized in that it comprises the steps consisting of: detecting at least one instrument (1; 2; 3) present at the operative site, detecting at least one portion of an organ (4) in the vicinity of the detected instrument (1; 2; 3), determining the positioning of the detected instrument (1; 2; 3) relative to the detected organ (4), so that the detected instrument (1; 2; 3), the detected portion of organ (4) and their relative positioning form parameters defining a detected surgical step, selecting, in a database, a conceptual surgical step corresponding to the detected surgical step from to the detected instrument (1; 2; 3), the detected portion of organ (4) and their relative positioning, comparing the parameters of the detected surgical step with the parameters defining the selected conceptual surgical step to determine the differences betwee
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: May 16, 2017
    Assignee: Universite Grenoble Alpes
    Inventors: Philippe Cinquin, Sandrine Voros, Pierre Mozer, Aurel Messas, Jean-Alexandre Long, Alexandre Moreau-Gaudry
  • Patent number: 9523629
    Abstract: A method and apparatus are provided for forming high-yield tissue microarray blocks capable of producing 1,000 or more replicate slides. In one embodiment, a plurality of donor tissue samples are minced and suspended in separate molten liquid carriers to form a plurality of separate two-phase mixtures of solid minced tissue and molten wax. Each of the two-phase mixtures is preferably formed into elongated columns and transferred into a separate well of a microarray block. A method and apparatus are provided for forming the elongated molten wax columns with minced tissue evenly distributed along the length of each column. The apparatus for forming the array includes an extrusion mechanism for transferring the columns of two-phase mixtures into the deep wells of the microarray block. The two-phase mixture may alternately be cooled and allowed to solidify before being transferred into the microarray block.
    Type: Grant
    Filed: December 16, 2014
    Date of Patent: December 20, 2016
    Assignee: Array Sciences, LLC
    Inventors: Regan Spencer Fulton, William Scott Crawford
  • Patent number: 9506928
    Abstract: Provided herein are methods, compounds, mixtures and formulations of antigen retrieval agent useful in retrieving antigens and improving the detection of amino acids, peptides and proteins or epitopes thereon in a tissue fixed with aldehyde-based cross-linking agents. Contacting the fixed tissue with a solution of the aldehyde scavenging agent causes reactivity with the aldehyde moieties to retrieve antigens and improve detection of the amino acids, peptides and proteins or epitopes. Also provided are kits comprising the antigen retrieval agent and, optionally, components for staining or detecting the proteins or the antigens or epitopes and instructions for using the kit. Further provided is a method for identifying an antigen retrieval agent. A fixed protein is contacted with an agent to be tested and heated in solution therewith. Detection of protein peaks via mass spectrometry indicates the tested agent is an antigen retrieval agent.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: November 29, 2016
    Assignee: UNIVERSITY OF HOUSTON SYSTEM
    Inventors: Jason Eriksen, Craig Vollert, Steven Bark, Wilna Moree
  • Patent number: 9470694
    Abstract: The present invention relates a simple method for evaluating free eukaryotic cell nuclei for biomarkers of DNA damage and/or transcription factor activation, activity, or expression levels and/or epigenetic modifications to chromatin or chromatin-associated factors. The invention also teaches useful strategies for combining nuclear biomarkers into a matrix of endpoints that are capable of elucidating genotoxicants' primary mode of DNA-damaging activity. Kits for conducting methods according to the invention are also described.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: October 18, 2016
    Assignee: Litron Laboratories Ltd.
    Inventors: Stephen D. Dertinger, Steven M. Bryce, Jeffrey C. Bemis
  • Patent number: 9429520
    Abstract: The present invention relates to enrichment and/or identification of fetal cells of a maternal blood sample using fetal cell specific ligands and/or fetal cell specific hybridization probes wherein the ligand or probes are directed to an endothelial/mesenchymal marker, e.g. CD105, CD146 or CD141, in a first round of enrichment and the ligand or probes, in a second round of enrichment, are directed to an epithelial marker, e.g. a cytokeratin, such as CK7, CK8, CK18 or CK19. Enriched or identified fetal cells may be subjected to steps of detection or diagnosis, wherefore the present invention enables non-invasive 5 prenatal diagnostics.
    Type: Grant
    Filed: November 9, 2011
    Date of Patent: August 30, 2016
    Assignee: Arcedi Biotech ApS
    Inventors: Andreas Eckelt, Britta Christensen, Steen Kolvraa, Marie Brinch, Ripudaman Singh, Lotte Hatt
  • Patent number: 9320995
    Abstract: Methods and systems for treating material with focused acoustic energy and/or ultraviolet radiation are described. Sample material may be provided to a single batch vessel or may flow in a continuous or intermittent fashion into/out of a processing chamber within which the material is exposed to focused acoustic energy and/or ultraviolet radiation. Exposure to focused acoustics in a flow through environment may enhance the effects of the radiation on the sample material. Further, in flow-through or single batch arrangements, sample material may be retained exterior to or within a processing chamber by one or more retaining members (e.g., filter, valve, etc.) until a characteristic of the sample material reaches one or more particular criteria (e.g., physical, chemical, biological). Once the criteria of the sample material is attained (e.g., via further treatment), the material is then permitted to flow past the retaining member(s) and through the inlet or outlet of the chamber.
    Type: Grant
    Filed: September 17, 2013
    Date of Patent: April 26, 2016
    Assignee: Covaris, Inc.
    Inventors: James A. Laugharn, Jr., Xiaoyin He, Srikanth Kakumanu
  • Patent number: 9221250
    Abstract: The invention provides apparatuses and methods for acoustically ejecting the fluid from a reservoir contained in or disposed on a substrate. The reservoir has a portion adapted to contain a fluid, and an acoustic radiation generator is positioned in acoustic coupling relationship to the reservoir. Acoustic radiation generated by the acoustic radiation generator is transmitted through at least the portion of the reservoir to an analyzer. The analyzer is capable of determining the energy level of the transmitted acoustic radiation and raising the energy level of subsequent pulses to a level sufficient to eject fluid droplets from the reservoir. The invention is particularly suited for delivering fluid from a plurality of reservoirs in an accurate and efficient manner.
    Type: Grant
    Filed: May 20, 2014
    Date of Patent: December 29, 2015
    Assignee: LABCYTE INC.
    Inventors: Richard G. Stearns, Shehrzad Ahmed Qureshi
  • Patent number: 9175313
    Abstract: The present invention provides a three-dimensional structures of P. tremuloides isoprene synthase and P. alba isoprene synthase. The invention also provides methods of using the three-dimensional structure to design isoprene synthases with improved activity for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.
    Type: Grant
    Filed: May 6, 2013
    Date of Patent: November 3, 2015
    Assignees: Danisco US Inc., The Goodyear Tire & Rubber Company
    Inventors: Zachary Q. Beck, Richard R. Bott, Jeffrey V. Miller, Christopher L. Rife, Derek H. Wells
  • Patent number: 9176149
    Abstract: Provided herein are methods and devices for the detection of conditions or disorders by detecting altered levels of stress response pathway biomarkers. Also provided are methods and reagents for identifying panels of biomarkers associated with a condition or disorder.
    Type: Grant
    Filed: July 7, 2014
    Date of Patent: November 3, 2015
    Inventor: Sarka O. Southern
  • Patent number: 9175330
    Abstract: A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins. The artificial genetic circuit detects isoprene liberated from many enzymatic reactions and measures the activity of reporter genes. This system is widely applicable for high throughput and quantitative screening of isoprene biosynthesis enzymes and MEP/MVA pathway enzymes. Therefore, the invention can be advantageously used in the protein engineering technology for enzyme modification.
    Type: Grant
    Filed: February 20, 2013
    Date of Patent: November 3, 2015
    Assignee: KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
    Inventors: Seung-Goo Lee, Seo Hyeon Kim, Jongsik Gam, Eugene Rha, Haseong Kim, Su-Lim Choi, EuiSung Choi
  • Patent number: 9109984
    Abstract: Various embodiments of the present invention provide methods and systems for optimally staining biological samples for analysis. In one embodiment, images of a sample are recorded before and after the application of a staining reagent and a decolorization reagent. A difference image is then generated based at least in part on a comparison of the images of the sample recorded before and after the application of the staining and decolorization reagents. Application parameters of the staining and decolorization reagents are then corrected based at least in part on the difference image such that the reagents may be optimally reapplied to generate a final image of the sample that may enable a user to better differentiate at least one target of interest in the sample. In one example, embodiments of the present invention allow for the generation of images that show only Gram-positive bacteria or only Gram-negative bacteria.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: August 18, 2015
    Assignee: Becton, Dickinson and Company
    Inventor: Klaus W. Berndt
  • Patent number: 9091620
    Abstract: The present application discloses methods and apparatus for preparing blood film or smear samples on a fiber surface, staining the test sample, and generating digital cell images of the films or samples for classification and review. The techniques disclosed herein replace conventional microscope slides with fibers when preparing blood film test samples. Using fibers to prepare blood test samples facilitates automation and integration of the various processes involved in hematology analysis. The techniques disclosed herein can be used to improve the accuracy and efficiency of hematology analysis.
    Type: Grant
    Filed: April 22, 2013
    Date of Patent: July 28, 2015
    Inventor: Chen Yi
  • Patent number: 9050422
    Abstract: The invention includes compositions of stem and progenitor cells recovered from bone marrow or cord blood containing most of the viable CD34+ cells and substantially depleted of red blood cells resident in the original sample, without any xenobiotic additives to aid cell separation. The invention also includes a system and method for preparing the compositions. The system includes a bag set and a processing device, which utilizes an optical sensor, microcontroller, servo motor, accelerometer, load cell, and battery. The system and method utilize centrifugation to stratify the cells into layers and then separate and transfer the stem cells into a stem cell bag. The processing device's microcontroller receives input from the device's accelerometer, load cell and optical sensor to direct the metering valve in the bag set to open and close to permit the transfer of as many stems cells as possible with as few red cells as possible.
    Type: Grant
    Filed: March 15, 2012
    Date of Patent: June 9, 2015
    Assignee: Cesca Therapeutics
    Inventors: Philip H. Coelho, Bruce A. Baker, John R. Chapman, Junzhi Li, Prince Emmanuel, Robert S. Childers
  • Publication number: 20150147779
    Abstract: A clearing agent and mounting solution for microscopy is disclosed comprising (a) trichloroethanol, (b) optionally, trichloroacetic acid, (c) optionally, glycerol and (d) optionally, water, where the refractive index of the solution is greater than or equal to about 1.3810. The solution can further comprise a C1-C6 alcohol, other acids, and/or a stain. The solution can also comprise derivatives and/or analogs of 2,2,2-trichloroethanol and/or trichloroacetic acid. Also disclosed is a method of preparing specimens for microscopy comprising (a) applying a specimen to a microscope slide or a cuvette, (b) applying a quantity of the above solution sufficient to mount the specimen, and (c) optionally applying a cover slip. The solution can be used effectively with stains or dyes, and with fresh, partially dry or dried materials, and for temporary or semi-permanent to permanent mounting.
    Type: Application
    Filed: April 9, 2013
    Publication date: May 28, 2015
    Inventors: James E. Simon, Thomas Villani, Adolfina Koroch
  • Publication number: 20150148243
    Abstract: A photocleavable polymer comprising a photocleavable linker and a polymer having a functional group that binds to a protein, lipid, sugar of a cell, or any combination thereof, as well as a method of selectively lysing cells by incubating a sample comprising two or more cells with the photocleavable polymer to reversibly fix the cells; selectively irradiating the fixed cells or a target or non-target cell among the fixed cells to cleave the photocleavable polymer; and adding a cell lysis solution to selectively lyse the irradiated cells.
    Type: Application
    Filed: July 3, 2014
    Publication date: May 28, 2015
    Inventors: Hun-joo LEE, Yeon-jeong Kim, Dong-hyun Park, Jong-myeon Park
  • Publication number: 20150147778
    Abstract: A sample vitrification system includes a capsule structure configured for carrying a biological sample within a compartment while the sample is subjected to ultra-rapid freezing by way of a cryogenic coolant jet, and while the sample is exposed to pulsed microwave radiation in a manner that disrupts water molecule pentamer formation and which disrupts initial ice crystal nucleation events within the compartment within tens of microseconds to provide a vitrification depth within the compartment of tens of microns or more. The sample can reside between a very high thermal conductivity substrate and a cover. The cryogenic coolant jet is applied to the substrate from beneath the sample. The cover can carry a set of microwave excitation elements configured for providing microwave radiation to internal portions of the compartment. Portions of the capsule structure's interior can be imaged during microwave assisted jet freezing, such as by way of an optical microscope.
    Type: Application
    Filed: April 4, 2013
    Publication date: May 28, 2015
    Inventor: Daniel Shawn Pickard
  • Publication number: 20150140601
    Abstract: A method (100) for fixing at least one tissue sample (1) is carried out using a tissue processor (10). The tissue sample (1) is introduced at a first temperature level and a first pressure level into a fixing reagent (2) containing at least one nitrogen-containing compound capable of pressure- and/or temperature-dependent release of at least one aldehyde. The tissue sample (1) is left in the fixing reagent (2) for a fixing time period during which the fixing reagent (2) is brought to a second temperature level above the first temperature level and/or to a second pressure level above the first pressure level. A concentration in the fixing reagent (2) of the nitrogen-containing compound is ascertained using at least one concentration measuring device (14) of the tissue processor (10), and a signal is outputted on the basis of a measured value of the at least one concentration measuring device (14).
    Type: Application
    Filed: November 4, 2014
    Publication date: May 21, 2015
    Inventors: Hermann Ulbrich, Markus Berberich
  • Publication number: 20150132797
    Abstract: Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups.
    Type: Application
    Filed: July 16, 2014
    Publication date: May 14, 2015
    Inventors: Brian FILANOSKI, Lawrence GREENFIELD, James HIRSCH
  • Publication number: 20150132798
    Abstract: The invention provides an integrated sequential sample preparation system using a sequential centrifuge for preparing samples for analysis. Methods of more efficiently preparing discrete samples sequentially for subsequent analysis are also provided. The apparatus and methods for sequentially preparing discrete samples provide improved operating efficiencies over conventional preparation processes that use batch centrifugation systems. Such advantages include reducing dwell time, increasing system throughput, reducing sample preparation system footprint, and improving precision of the analytical process. The integrated sequential preparation system with the integrated sequential centrifuge further provides the capability of handling critical or STAT samples without compromising the operating efficiencies achieved by preparing discrete samples in a sequential manner.
    Type: Application
    Filed: January 9, 2015
    Publication date: May 14, 2015
    Inventors: William Alan Fox, Charles L. Carrico, JR.
  • Publication number: 20150125875
    Abstract: Methods for producing an isotope-labeled mammalian, including a human, biomolecule, such as polypeptides and proteins, in a cell-free protein synthesis system. A biomolecule standard is produced having at least one isotope different in abundance than that of the naturally occurring isotopes in the biomolecule. Methods for quantifying biomolecules standards expressed using mammalian cell-free extracts are disclosed. Methods for producing such standards, kits, systems and reagents, relating to the use of isotope-labeled biomolecule as quantification standards in mass spectrometric and nuclear magnetic resonance analysis.
    Type: Application
    Filed: December 26, 2014
    Publication date: May 7, 2015
    Applicant: PIERCE BIOTECHNOLOGY, INC.
    Inventors: Ryan D. Bomgarden, Eric Leigh Hommema, John Charles Rogers, Penny JoAnn Jensen, Derek Karl Baerenwald
  • Publication number: 20150126402
    Abstract: A method for quantifying metallothionein protein isomers is described herein. Such metallothionein isomer protein quantification is useful for detecting and monitoring disease. As illustrated herein, protein quantification is a more accurate measure of metallothionein induction than is mRNA quantification.
    Type: Application
    Filed: May 17, 2013
    Publication date: May 7, 2015
    Inventors: John B. Shabb, Aaron Mehus, Wallace Muhonen, Donald A. Sens, Scott Garrett