Involving Tissue Sections Patents (Class 435/40.52)
  • Patent number: 10830675
    Abstract: This disclosure relates to hematoxylin staining formulations and particularly to formulations for use in autostainers. The disclosed compositions were discovered to possess atypical stability under storage while having high stain quality and sufficiently fast staining performance. The disclosed hematoxylin staining compositions include a solvent system, hematoxylin, a chemical oxidant, and a mordant. Illustrative embodiments also have a Cl?/SO4? molar ratio of between about 2.5/1 and about 1/4.
    Type: Grant
    Filed: June 19, 2017
    Date of Patent: November 10, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Heidi Barnett, Edward E. Durrant, Eric L. Klein, Michael Lynch, Kevin H. Pritchard
  • Patent number: 10816444
    Abstract: Disclosed are specimen processing systems capable of processing specimens carried on slides. The specimen processing systems comprise opposables having at least one fluid control element. The fluid control elements may be positioned between spacers or gapping elements the opposable edges. The fluid control elements may comprise an edge, such as a beveled edge or a stepped edge, as described herein, and the edge may be continuous or segmented.
    Type: Grant
    Filed: December 23, 2017
    Date of Patent: October 27, 2020
    Assignee: Ventana Medical Systems, Inc.
    Inventors: Donald Barnett, Chad Wilkinson, Brian Howard Kram, Kevin David Marshall, Christine Tse, Timothy James Keller, Austin Micheil Ashby
  • Patent number: 10539487
    Abstract: A tissue sample that has been removed from a subject can be evaluated. A change in speed of the energy traveling through the sample is evaluated to monitor changes in the biological sample during processing. The monitoring can detect movement of fluid with the sample and cross-linking. A system for performing the method can include a transmitter that outputs the energy and a receiver configured to detect the transmitted energy.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: January 21, 2020
    Assignee: VENTANA MEDICAL SYSTEMS, INC.
    Inventors: Jefferson Curtis Taft, David Chafin, Michael Otter, Benjamin Stevens
  • Patent number: 10481054
    Abstract: The invention relates to a method for embedding a tissue sample into an embedding medium. In a first step the tissue sample is held in an intended orientation in a container by means of a holding element that presses the tissue sample against a base of the container. In a further step a liquid embedding medium that has a temperature above 64 degrees Celsius, in particular in the range from 65 degrees Celsius to 67 degrees Celsius, or a temperature of 66 degrees Celsius, is poured into the container, and the base of the container is cooled.
    Type: Grant
    Filed: September 1, 2016
    Date of Patent: November 19, 2019
    Assignee: Leica Biosystems Nussloch GmbH
    Inventors: Florian Böhl, Ralf Eckert, Karin Flieger, Markus Berberich, Hermann Ulbrich, Stella Knorr, Andrew Guy
  • Patent number: 10482998
    Abstract: The present disclosure relates to cell processing techniques. By way of example, a cell processing system may include a plurality of tracking devices coupled to a respective plurality of sample processing containers and storing sample identification information for a patient sample for use with sample processing devices configured to process the patient sample according to workflow steps of a processing protocol. A plurality of readers are configured to receive signals from the plurality of tracking devices. A controller may be coupled to the plurality of readers and configured to determine if the patient sample is processed according to the processing protocol based at least in part on the signals from the plurality of tracking devices.
    Type: Grant
    Filed: December 30, 2015
    Date of Patent: November 19, 2019
    Assignee: General Electric Company
    Inventors: Nichole Lea Wood, Lynn Ann DeRose, Brandon Stephen Good, Adam Joseph McCann, Hasan Imamus Saleheen
  • Patent number: 10151675
    Abstract: A method for increasing the optical transparency of regions of a tissue sample (1) is proposed, in which the tissue sample (1) is introduced into a process chamber (10) and is infiltrated in the process chamber (10) with at least one process fluid (2), and in which a removal of light-scattering structures in the tissue sample (1) is carried out. The method encompasses monitoring the optical transparency of the tissue sample (1), at least during a clearing time period in which the tissue sample (1) is introduced into the process chamber (10) and in which the removal of the light-scattering structures in the tissue sample (1) is carried out, by means of an optical transparency measuring arrangement (13) associated with the process chamber (10). An apparatus (100) for carrying out the method is also a subject of the invention.
    Type: Grant
    Filed: August 26, 2014
    Date of Patent: December 11, 2018
    Assignee: Leica Microsystems CMS GmbH
    Inventor: Bernd Sägmüller
  • Patent number: 9645056
    Abstract: Disclosed are a pretreatment solution for immunohistochemical staining, which elutes a paraffin-containing embedding medium from a glass slide with a tissue specimen embedded in the medium, and retrieves antigenicity of the tissue specimen, and which is usable three or more times, and a pretreatment solution concentrate for immunohistochemical staining which allows ready preparation of the pretreatment solution and a method of immunohistochemical staining using the same. The pretreatment solution contains an antigen retrieving agent, particular nonionic surfactants, and cyclodextrin or a derivative thereof, with the balance being not less than 80 mass % of water. The content of the antigen retrieval agent is such that the pH of the pretreatment solution is in a predetermined range, and the content of cyclodextrin or a derivative thereof is a particular amount.
    Type: Grant
    Filed: June 9, 2014
    Date of Patent: May 9, 2017
    Assignee: NICHIREI BIOSCIENCES, INC.
    Inventor: Toshiyuki Kasamatsu
  • Patent number: 9194776
    Abstract: The invention relates to a processor for working on histological samples. The processor has an inlet for the insertion of a fixing container, particularly a closed fixing container, with the samples located in the latter.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: November 24, 2015
    Assignee: Leica Biosystems Nussloch GmbH
    Inventors: Ralf Eckert, Hermann Ulbrich, Stella Knorr, Graeme Robertson
  • Patent number: 9023614
    Abstract: A method for rapidly collecting cells from a surface, such as a surface bearing fingerprints, for subsequent macromolecular analysis involves dispensing a predetermined amount of an aqueous solution onto the surface, and subjecting the aqueous solution to ultrasound waves to promote a detachment of the cells from the surface. Extraction of macromolecules such as DNA from the cells can be effected directly in the solution containing the collected cells by further subjecting the solution to ultrasound waves for a prescribed period of time to lyse the cells, and then extracting the DNA.
    Type: Grant
    Filed: July 9, 2004
    Date of Patent: May 5, 2015
    Inventor: Tofy Mussivand
  • Publication number: 20150111202
    Abstract: An apparatus including a reagent cartridge and a reaction chamber, the reagent cartridge having a reagent capsule removably positioned therein for dispensing of a reagent onto the reaction chamber. A system including a linearly translatable mounting assembly having a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge, a linearly translatable bulk reagent dispensing assembly having a plurality of bulk reagent dispensing nozzles coupled thereto and a receiving assembly positioned beneath the mounting assembly and the bulk reagent dispensing assembly, the receiving assembly including a plurality of reaction stations.
    Type: Application
    Filed: December 22, 2014
    Publication date: April 23, 2015
    Inventors: Xuan S. Bui, Anthony L. Hartman, Toshiyuki Fujimaki, Shinji Tokudaiji, Yoshitake Okabe, Yoshitada Mizusawa
  • Publication number: 20150099669
    Abstract: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement.
    Type: Application
    Filed: May 23, 2013
    Publication date: April 9, 2015
    Inventors: Hui Zhang, Xingde Li, Shadi Toghi Eshghi
  • Publication number: 20150093780
    Abstract: The invention relates to the preparation of thin tissue sections for mass spectrometric (MALDI) imaging, and proposes a method wherein a microcrystalline layer of the matrix material is produced on the surface of the thin tissue sections, and soluble analyte molecules are transported orthogonally through the thin tissue section to the matrix layer, without substantial lateral diffusion, by means of a solvent flow with alternating or constant direction, and are then deposited in the matrix layer and, if possible, embedded in the matrix crystals. A solvent flow which alternates in direction can be produced by successive, alternating phases of swelling and drying, brought about by periodic changes to the thin tissue section temperature and/or the partial pressures of the solvents. A continuous solvent flow can be generated by applying the thin tissue section onto a porous support which supplies solvent to the rear surface of the thin tissue section.
    Type: Application
    Filed: September 29, 2014
    Publication date: April 2, 2015
    Inventors: Martin SCHÜRENBERG, Jochen FRANZEN, Kai RÜCKRIEM, Eckhard BELAU
  • Publication number: 20150087001
    Abstract: In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.
    Type: Application
    Filed: July 30, 2014
    Publication date: March 26, 2015
    Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY
    Inventors: Viviana Gradinaru, Bin Yang
  • Publication number: 20150087019
    Abstract: A method of avoiding deformation and providing for a more consistent thickness of a biological tissue sample during preparation for analysis comprising providing a histology processing cassette comprising a box defining a compartment for holding a biological tissue sample, the box having a bottom face comprising a sample support surface and being transmissible to radiation or a flow of fluid through to pass through the cassette, an open top face, and two side walls, a back wall and a front wall, the box having a length and width greater than a standard size histology processing cassette and a depth approximately the same as a standard size histology processing cassette placing the sample in the cassette and treating the sample by contacting it with a processing solution; and contacting the sample with molten paraffin wax and cooling the sample in molten paraffin wax so the sample is embedded in solidified paraffin wax for further processing.
    Type: Application
    Filed: September 23, 2014
    Publication date: March 26, 2015
    Inventors: Paul Webber, Philip Webber, Richard Titcombe
  • Patent number: 8975039
    Abstract: Provided is a system and method for staining of one or more samples, including providing one or more self-contained sample processing receptacles, each of the one or more self-contained sample processing receptacles configured to be inserted into an auto-staining instrument; and enabling one of one or more staining procedures appropriate for the one or more samples as a function of a choice of self-contained sample processing receptacle, each of the one or more self-contained sample processing receptacles configured to process each inserted sample of the one or more samples within the self-contained sample processing receptacle.
    Type: Grant
    Filed: June 9, 2008
    Date of Patent: March 10, 2015
    Assignee: SYFR, Inc.
    Inventors: Shazi Iqbal, Steven M. Montgomery, Ronald Chang, Gregory Mote, Douglas B Dority, Jeffrey S. Ross
  • Patent number: 8969100
    Abstract: Regions where metastatic cancer cells can exist are detected with high accuracy in a sentinel lymph node. Quantum dots are injected into the vicinity of a cancer in a living body, thereby identifying the location of the sentinel lymph node by means of fluorescence. Subsequently, the sentinel lymph node is extracted. With respect to the sentinel lymph node extracted with quantum dots injected, structural analysis is conducted by means of precision fluorescence measurement which uses a confocal fluorescence microscope for monomolecular observation. Specifically, the fluorescence intensity is measured with respect to each of multiple areas in the sentinel lymph nodes, and out of the multiple areas measured, one or more areas are detected as afferent lymph vessel inflow regions in descending order of fluorescence intensity.
    Type: Grant
    Filed: March 11, 2010
    Date of Patent: March 3, 2015
    Assignees: Tohoku University, Konica Minolta Medical & Graphic, Inc.
    Inventors: Makoto Hikage, Kohsuke Gonda, Motohiro Takeda, Takashi Kamei, Noriaki Ohuchi, Hideki Gouda, Yasushi Nakano
  • Publication number: 20150056650
    Abstract: A microfluidic apparatus, method, and associated applications utilize and apply to a formalin-fixed paraffin-embedded (FFPE) tissue sample and performing a liquid-liquid extraction to remove the paraffin from the tissue sample prior to a nucleic acid purification step. A microfluidic device includes a dedicated liquid-liquid extraction process vessel, a nucleic acid purification process component, and a nucleic acid amplification reactor. A liquid-liquid extraction and nucleic acid purification kit includes a microfluidic device capable of performing both a liquid-liquid extraction process and a nucleic acid purification process, including a dedicated liquid-liquid extraction process vessel, an immiscible liquid or a precursor phase thereof disposed in the vessel, a nucleic acid purification process component, a nucleic acid amplification reactor fluidically, and a supply of reagents suitable to enable the liquid-liquid extraction process and the nucleic acid purification process.
    Type: Application
    Filed: September 5, 2014
    Publication date: February 26, 2015
    Applicant: RHEONIX, INC.
    Inventor: Peng Zhou
  • Publication number: 20150050689
    Abstract: A fixation agent including at least one polyamine and at least one acidification agent. The quality and staining of tissue samples or tissue sections that have not been fixed with formalin is improved, with higher brilliance and color stability of the stain, allowing the tissue to be cut with a better quality without artifacts.
    Type: Application
    Filed: March 1, 2013
    Publication date: February 19, 2015
    Inventor: Roberto Gerigk
  • Publication number: 20150050690
    Abstract: In order to pick up a sufficient amount of cells for a genetic test from a section of a biological tissue with a simple structure and a simple operation, provided is a cell collection apparatus comprising: a substrate which is provided to be dividable into a plurality of small pieces along a predetermined dividing line, and which has a flat surface to which a section of a biological tissue can be pasted; a sheet-shaped expandable member to which the substrate can be adhered in a detachable manner, and which is expandable in a direction along the surface; a expansion unit for expanding the expandable member in at least a region adhered with the substrate, in a direction along the surface; and a pickup unit for detaching and picking up the divided small pieces from the expandable member.
    Type: Application
    Filed: October 30, 2014
    Publication date: February 19, 2015
    Applicant: OLYMPUS CORPORATION
    Inventor: Nobuhiko Morimoto
  • Patent number: 8940528
    Abstract: A dish for cell cultivation and microscopy is provided, comprising a ring and an outer ridge upwardly and axially protruding therefrom, wherein the ring is covered at its bottom side with a transparent membrane and, thus, forms a recess delimited by the inner side of the ring and the membrane, characterized in that the recess is filled with an adhesive filling. Alternatively, a dish for cell cultivation and microscopy is provided, comprising a base and an outer ridge protruding upwardly and axially at its rim, wherein the base and the outer ridge are made of a transparent plastic, the base together with the inner side of the outer ridge forms a recess filled with an adhesive filling, and the thickness of the base and the filling together is less than about 1 mm.
    Type: Grant
    Filed: July 20, 2009
    Date of Patent: January 27, 2015
    Assignee: Molecular Machines & Industries AG
    Inventor: Stefan Niehren
  • Publication number: 20150005190
    Abstract: A biological and chemical sample processing device that b. comprises a high pressure-resistant, shallow and wide area microfluidic chamber having at least one wall formed by a detachable slide containing samples such as immobilized entities, biological samples or molecules, c. comprises an arrangement of microfluidic access holes for injecting to and collecting fluid form said chamber, d. is interfaced with inlet ports and microfluidic channels which are formed external to the chamber, e. is configured so that the slide may be brought into contact with the device to form the said chamber, f. is adapted to deliver and to transport fluidic substances and reagents inside said chamber in a fast manner, preferably within less than 15 seconds, and in a regular or uniform way owing to said arrangement of microfluidic access holes.
    Type: Application
    Filed: February 15, 2013
    Publication date: January 1, 2015
    Inventors: Ata Tuna Ciftlik, Martin Gijs
  • Publication number: 20150004630
    Abstract: Methods for cell-based tissue analysis utilize modern trends in digital microscopy to obtain, process, calibrate, and analyze digital images of tissue sections to quantify cell-based data for improved histological analysis. Using data from multiple images of a common tissue section, or data from images of multiple tissue sections, additional degrees of freedom are realized and the resulting analysis provides added depth to histological analysis of tissue samples. With computerized analytical methods, speed and accuracy of histological analysis is greatly improved.
    Type: Application
    Filed: July 3, 2013
    Publication date: January 1, 2015
    Applicant: FLAGSHIP BIOSCIENCES, LLC
    Inventors: Holger Lange, Josheph Krueger, George Young D., Trevor Johnson, Frank Voelker, Steven Potts
  • Publication number: 20140356876
    Abstract: In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue, capturing the sectioned tissue, and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, and capturing each sectioned tissue, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
    Type: Application
    Filed: June 10, 2014
    Publication date: December 4, 2014
    Inventor: Timothy Ragan
  • Publication number: 20140335560
    Abstract: Systems and methods for automated laser microdissection are disclosed including automatic slide detection, position detection of cutting and capture lasers, focus optimization for cutting and capture lasers, energy and duration optimization for cutting and capture lasers, inspection and second phase capture and/or ablation in a quality control station and tracking information for linking substrate carrier or output microdissected regions with input sample or slide.
    Type: Application
    Filed: May 12, 2014
    Publication date: November 13, 2014
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Thomas M. BAER, Michael G. YOUNGQUIST, Brian W. DONOVAN, Alan E. WESSEL, Norbert H. LECLERC, Michael A. SMITH, Craig S. BARKER, George M. DAWSON
  • Publication number: 20140323406
    Abstract: The invention provides methods of treating stroke and related conditions exacerbated by fever and/or hyperglycemi by administering peptides or peptidomimetics that inhibit binding of NMDAR 2B to PSD-95 to a patient.
    Type: Application
    Filed: February 7, 2014
    Publication date: October 30, 2014
    Applicant: NONO INC.
    Inventor: Michael Tymianksi
  • Publication number: 20140302552
    Abstract: A container for storing a biological sample is disclosed. The container includes a first chamber having a sidewall extending between an open end and a closed end, defining a first chamber interior adapted to receive a sample holder therein. The container also includes a second chamber having a sidewall extending between an open end and a closed end, defining a second chamber interior adapted to subsequently receive the sample holder therein. The second chamber interior is in fluid isolation from the first chamber interior. A removable closure encloses at least one of the open end of the first chamber and the open end of the second chamber while the sample holder is disposed within one of the first chamber interior and the second chamber interior. A first fluid may be disposed within the first chamber interior, and a second different fluid may be disposed within the second chamber interior.
    Type: Application
    Filed: June 19, 2014
    Publication date: October 9, 2014
    Inventors: Bradley M. Wilkinson, C. Mark Newby, Clinton A. Haynes, Robert G. States, III
  • Patent number: 8845623
    Abstract: A film with a tissue section is placed with the tissue section downward on a microscope slide and the microscope slide positioned in the object plane of an inverse microscope; where an adhesive tape is arranged, with a bonding agent downward, above the film and therefore above the tissue section; wherein the next step, tissue to be isolated is excised by a focused laser beam, which also divides the film; whereupon removal of the adhesive tape from the microscope, the excised tissue pieces adhere to the adhesive tape, and the remnant of the film and of the tissue section remains adhering to the microscope slide.
    Type: Grant
    Filed: February 6, 2007
    Date of Patent: September 30, 2014
    Assignee: Molecular Machines & Industries AG
    Inventor: Norbert Leclerc
  • Publication number: 20140287456
    Abstract: A method of removing a floatation liquid from between a microscope slide and a paraffin embedded biological specimen including position the microscope slide with the paraffin embedded biological specimen floated thereon onto a slide support element. The slide support element is rotated to cause the microscope slide and the paraffin embedded biological specimen to turn in a way that causes the floatation liquid disposed between the microscope slide and the paraffin embedded biological specimen to be drawn from between the microscope slide and the paraffin embedded biological specimen.
    Type: Application
    Filed: March 17, 2014
    Publication date: September 25, 2014
    Inventor: Lee H. Angros
  • Publication number: 20140273087
    Abstract: Systems, devices, and methods for removing areas of tissue are described. A programmable laser may remove precise areas of tissue while the tissue remains substantially frozen. The laser is programmed in part by analyzing a reference image of a representative tissue section. A software program may receive digital images of test slices. Areas of interest in the image may be selected by a user. The software program can then create and send cut instructions to the programmable laser. The laser may be configured to make perforated cuts to remove the area of interest without melting the removed section.
    Type: Application
    Filed: May 30, 2014
    Publication date: September 18, 2014
    Applicant: International Genomics Consortium
    Inventor: Scott Morris
  • Publication number: 20140273083
    Abstract: A method for treating a tissue sample including placing at least one tissue sample on an cassette which has: a retaining member, a base and at least one biasing element; placing the at least one tissue sample in the tissue cassette; attaching the base and the retaining member to retain the tissue sample; processing the tissue sample in the tissue cassette with one or more solvents; and embedding the tissue sample in a paraffin to form an portion of paraffin in which the tissue sample is embedded in the tissue cassette, wherein the embedding comprises adding molten paraffin to the interior area of the tissue cassette and allowing the paraffin to become solid.
    Type: Application
    Filed: March 11, 2014
    Publication date: September 18, 2014
    Applicant: LEICA BIOSYSTEMS NUSSLOCH GMBH
    Inventors: Stella KNORR, Andrew GUY, Ralf ECKERT, Fiona TARBET, Fernando DIAS, Chris RYAN, Neil SANUT
  • Publication number: 20140273084
    Abstract: An apparatus for holding a tissue sample having a retaining member with a first tissue engaging surface and at least one biasing element. The first tissue engaging surface is moveably attached to the retaining member. The apparatus also has a base comprising a second tissue engaging surface which is configured to engage the retaining member to form an interior area with the first and second tissue engaging surfaces facing each other. The apparatus also has a retracting member connected to the retaining member which is configured to retract the first tissue engaging surface and compress the biasing element to form a gap between the tissue sample and one of the first tissue engaging surface and the second tissue engaging surface.
    Type: Application
    Filed: March 11, 2014
    Publication date: September 18, 2014
    Applicant: LEICA BIOSYSTEMS NUSSLOCH GMBH
    Inventors: Florian BOEHL, Stella KNORR, Peter KOTLARSKI, Graeme ROBERTSON, Chris RYAN, Ian SOHN, Wilfred BAUWENS, Fernando DIAS
  • Publication number: 20140273085
    Abstract: The invention relates to a processor for working on histological samples. The processor has an inlet for the insertion of a fixing container, particularly a closed fixing container, with the samples located in the latter.
    Type: Application
    Filed: March 11, 2014
    Publication date: September 18, 2014
    Applicant: LEICA BIOSYSTEMS NUSSLOCH GMBH
    Inventors: Ralf ECKERT, Hermann ULBRICH, Stella KNORR, Graeme ROBERTSON
  • Publication number: 20140273086
    Abstract: An apparatus including at least one of a stainer module and a coverslipper module; an imaging module; a storage module; an automated transport module for transporting at least one slide between at least one of the stainer module and the coverslipper module, the imaging module and the storage module; and a controller. A method including processing at least one slide; determining whether an imaging module is available for imaging of a biological specimen on the at least one slide; transporting the at least one slide to the imaging module using an automated transport module; and transporting the at least one slide to a storage module using the automated transport module when it is determined that the imaging module is not available. A system including a processing module for processing at least one slide including a biological specimen thereon. A machine readable medium.
    Type: Application
    Filed: May 28, 2014
    Publication date: September 18, 2014
    Applicant: SAKURA FINETEK U.S.A., INC.
    Inventor: Gilles Lefebvre
  • Publication number: 20140273088
    Abstract: A method, an automated apparatus and a system for staining of a plurality of biological samples arranged on slides, e.g. for histological and cytological examination. A patient case having slides arranged in a frame is loaded into the apparatus, in which pre-treatment, including e.g. drying, baking, dewaxing and target retrieval, as well as staining, is carried out differently in respect of the slides in accordance with predetermined or operator-specified treatment protocols.
    Type: Application
    Filed: October 12, 2012
    Publication date: September 18, 2014
    Inventor: Lars Winther
  • Publication number: 20140255985
    Abstract: An endscopic imaging device is described that achieves longitudinal axis (z-axis) scanning into a tissue or sample, using a piezoelectric microactuator. In some configurations, additional lateral (xy-plane) scanning is also achieved, to allow for the creation of full three-dimensional imaging, ex vivo or in vivo. The techniques may be used to image and diagnosis allergic rhinitis and eosinophilic esophagitis in tissue.
    Type: Application
    Filed: May 20, 2014
    Publication date: September 11, 2014
    Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN
    Inventors: Kenn Oldham, Thomas D. Wang, Zhongyao Liu, Jing Yong Ye
  • Publication number: 20140255986
    Abstract: An automated machine for handling and embedding tissue samples contained on microtome sectionable supports. The machine includes an input member configured to hold a plurality of the microtome sectionable supports prior to a tissue embedding operation. An output member is configured to hold a plurality of the microtome sectionable supports after the tissue embedding operation. A cooling unit is configured to hold at least one of the microtome sectionable supports during the tissue embedding operation. A motorized carrier assembly is mounted for movement and configured to hold at least one of the microtome sectionable supports. The carrier assembly moves the support from the input member to the cooling unit and, finally, to the output member. A dispensing device dispenses an embedding material onto the microtome sectionable support and at least one tissue sample carried by the microtome sectionable support during the embedding operation.
    Type: Application
    Filed: May 23, 2014
    Publication date: September 11, 2014
    Applicant: BIOPATH AUTOMATION, L.L.C.
    Inventors: Warren P. Williamson, IV, Thomas J. Ward, Douglas P. Allen
  • Patent number: 8828709
    Abstract: The invention relates to a method with which proteins from formalin-fixed biological samples can be dissolved and subsequently quantified. The method makes it possible to extract intact full-length proteins from the samples and to conduct a subsequent analysis thereof.
    Type: Grant
    Filed: May 10, 2006
    Date of Patent: September 9, 2014
    Assignee: Qiagen GmbH
    Inventors: Peter Porschewski, Karl-Friedrich Becker
  • Publication number: 20140248659
    Abstract: A tissue processing system includes a plurality of processing stations. Each processing station includes a plurality of tissue receiving areas that are each configured to accommodate a tissue sample as well as physically isolate that tissue sample from other tissue samples at other receiving areas of the same processing station. Each processing station is configured to separately and individually process the tissue sample at each receiving area to either reduce or eliminate any potential for cross-contamination between the tissue samples undergoing processing at the same processing station. For each receiving area of each processing station, the system is configured to immerse a tissue sample at a particular receiving area in processing fluid for a pre-determined time according to a pre-defined protocol that is based upon parameters of that tissue sample.
    Type: Application
    Filed: May 9, 2014
    Publication date: September 4, 2014
    Applicant: Rushabh Instruments, LLC
    Inventor: Preyas Shah
  • Publication number: 20140242634
    Abstract: The present invention provide for solutions of a defined composition useful in a staining protocol, such as a hematoxylin and eosin staining protocol, when used at certain points of the staining protocol. The formulations of these defined solutions are such that carry-over of the solutions will not negatively impact, or preferably, will stabilize or favorably modify staining reagent solutions coming in contact with the solutions. In certain embodiments of the invention, solutions are buffered to maintain a specific pH that when carried-over—such as carried-over into hematoxylin—will not significantly influence the pH of the next staining reagent.
    Type: Application
    Filed: May 2, 2014
    Publication date: August 28, 2014
    Inventors: Stanley E. Hansen, Russell Myers, Ronald Paul, Audra Rasmussen, Kate Taylor
  • Publication number: 20140234894
    Abstract: The present invention relates to a method for measuring a biological value Vb in a liver wherein said method comprises the steps of: a/ applying an infrared radiation having at least a first wave number range between 2800 cm?1 and 3000 cm?1 to one or more portions, pi, of a sample of said liver, b/ detecting the intensity of the radiation after it has passed through each of one or more portions, p;, and generating a signal related to the detected intensity, c/ processing the generated signal(s) to calculate an average value va; d/ comparing said average value Va to a standard to obtain the biological value Vb.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 21, 2014
    Inventors: Francois Le Naour, Paul Dumas, Catherine Guettier
  • Publication number: 20140234895
    Abstract: Improved methods and systems for processing of solid tissue are described. The method may be performed manually or automatically. The system may have modules such as (i) a grossing module where a fresh tissue is sliced to prepare a tissue specimen, (ii) a hardening module that hardens the tissue specimen, (iii) an impregnating module that impregnates the tissue specimen that was hardened, and (iv) an embedding module that embeds a tissue specimen that was hardened and impregnated. Fresh (i.e., not fixed or frozen) tissue, which was excised to diagnose disease or to assess surgical treatment, is grossed to about 0.6 mm Preferably, the hardening of fresh tissue is initiated, but not completed, during grossing by contact with a chemical admixture. Preferably, dry ice, a thermoelectric device, or a gas condenser cools a metal mold containing the embedded specimen.
    Type: Application
    Filed: September 28, 2012
    Publication date: August 21, 2014
    Inventor: Azorides Morales
  • Publication number: 20140228246
    Abstract: The present disclosure relates to an Extra Cellular Matrix composition specific for cancer type and a tumor microenvironment platform for long term culturing of tumor tissue, wherein said culturing provides human ligands and tumor tissue micro-environment to mimic physiologically relevant signalling systems. The present disclosure further relates to the development of a Clinical Response Predictor and its application in the prognostic field (selection of treatment option for the patient) and translational biology field (development of anticancer drugs). The disclosure further relates to a method of predicting clinical response of a tumor patient to drug(s). The disclosure further relates to a method for screening tumor cells for the presence of specific markers for determining the viability of said cells for indication of tumor status.
    Type: Application
    Filed: October 4, 2012
    Publication date: August 14, 2014
    Inventors: Mallikarjun Sundaram, Pradip Majumder, Biswanath Majumder, Misti Jain, Saravanan Thiagarajan, Dency Pinto, Padhma Radhakrishnan
  • Publication number: 20140220624
    Abstract: A protein retrieval treatment system used for activating proteins contained in a deparaffinized tissue section obtained by removing paraffin from a formalin-fixed paraffin-embedded tissue section. The protein retrieval treatment system includes: a dispensing unit for dispensing a retrieval treatment solution over a dispensing area including a measurement area on the deparaffinized tissue section; and a moist-heat treatment unit for heating, in a saturated water vapor, the deparaffinized tissue section with the dispensing area covered with the retrieval treatment solution.
    Type: Application
    Filed: April 25, 2012
    Publication date: August 7, 2014
    Applicants: SHIZUOKA PREFECTURE, SHIMADZU CORPORATION
    Inventors: Yutaka Aoki, Yukari Umino, Taka-Aki Sato, Isamu Hayashi, Keiichi Hatakeyama, Shun-ichiro Ogura
  • Patent number: 8790892
    Abstract: The invention discloses new substituted anthraquinone dyes that may be useful as cellular stains. In some aspect of the invention, the nuclear stains are useful for staining the nuclei of dead or fixed cells. Another aspect of the invention relates to substituted anthraquinone dyes comprising an enzyme substrate moiety that is transformable or cleavable by an enzyme such that the transformation or cleavage of the substrate moiety causes a detectable change in the functionality or spectral properties of the dye.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: July 29, 2014
    Assignee: Biotium, Inc.
    Inventors: Fei Mao, Wai-Yee Leung, Ching-Ying Cheung, Lori Michelle Roberts
  • Patent number: 8787651
    Abstract: Feature analysis on consecutive tissue sections (FACTS) includes obtaining a plurality of consecutive tissue sections from a tissue sample, staining the sections with at least one biomarker, obtaining a digital image thereof, and identifying one or more regions of interest within a middle of the consecutive tissue sections. The digital images of the consecutive tissue sections are registered for alignment and the one or more regions of interest are transferred from the image of the middle section to the images of the adjacent sections. Each image of the consecutive tissue sections is then analyzed and scored as appropriate. Using FACTS methods, pathologist time for annotation is reduced to a single slide. Optionally, multiple middle sections may be annotated for regions of interest and transferred accordingly.
    Type: Grant
    Filed: September 28, 2011
    Date of Patent: July 22, 2014
    Assignee: Flagship Biosciences, LLC
    Inventors: Steven Potts, Trevor Johnson, Frank Voelker, George David Young
  • Patent number: 8778630
    Abstract: A first permeable mesh membrane may be configured to be tautly stretched over a tissue sample and overlaid over a surface. A first permeable mesh membrane may sandwich and secure a tissue sample in between a first permeable mesh membrane and a surface. A first permeable mesh membrane may have a sieve size of approximately 10 ?m to approximately 100 ?m.
    Type: Grant
    Filed: October 6, 2011
    Date of Patent: July 15, 2014
    Assignee: George Mason Intellectual Properties, Inc.
    Inventors: Eswar Prasad Ramachandran Iyer, Daniel N. Cox
  • Patent number: 8771978
    Abstract: In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging layers of the tissue, sectioning a portion of the tissue, capturing the sectioned tissue, and imaging additional layers of the tissue in a second volume of the tissue, and capturing each portion of sectioned tissue, and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest. Further, captured tissue sections can be processed, re-imaged, and indexed to their original location in the three dimensional image.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: July 8, 2014
    Assignee: TissueVision, Inc.
    Inventor: Timothy Ragan
  • Publication number: 20140186883
    Abstract: The disclosure relates to a processor for processing histological samples. The processor comprises an inlet for insertion of a closed fixing container having the samples present therein.
    Type: Application
    Filed: December 22, 2013
    Publication date: July 3, 2014
    Applicant: Leica Biosystems Nussloch GmbH
    Inventors: Ralf Eckert, Markus Berberich, Hermann Ulbrich
  • Publication number: 20140186882
    Abstract: The disclosure relates to a method for fixing a histological sample, in which a target fixing time for the sample is defined and the sample is exposed to the action of a fixative, the exposure start time being stored in a memory associated with the sample. The sample is no longer exposed to the fixative when an action time of the fixative on the sample is equal to or greater than the target fixing time.
    Type: Application
    Filed: December 22, 2013
    Publication date: July 3, 2014
    Applicant: Leica Biosystems Nussloch GmbH
    Inventors: Markus Berberich, Ralf Eckert
  • Patent number: 8765401
    Abstract: A method and apparatus for producing histological sections by means of a microtome are suggested. A histological section of predefined thickness is produced with a microtome from a block comprising a tissue sample. A carrier material is applied with an application apparatus onto the block prior to before production of the histological section. To ensure that the histological sections do not roll up, and to enable improved downline processing of the histological sections as compared with a carrier material embodied in the form of an endless strip and that the carrier material is cut out prior to application to a size and/or a shape that corresponds substantially to the cross-sectional area of the block; and that the cut-out carrier material is then applied onto the block. The suggested apparatus is designed to apply the carrier material onto a histological section to be prepared by a microtome.
    Type: Grant
    Filed: December 22, 2008
    Date of Patent: July 1, 2014
    Assignee: Leica Biosystems Nussloch GmbH
    Inventors: Christoph Schmitt, Volker Schneider