Support Is A Suspendable Particle Patents (Class 435/403)
  • Patent number: 9057715
    Abstract: The present invention relates to a cell culture apparatus and a mass automatic cell culture device having it. There is provided a cell culture apparatus comprising a cylindrical cell culture flask which is air-tightly sealed and formed of a transparent material to observe an internal portion thereof, an injection unit for supplying the cell and the culture solution into the cell culture flask, a collection unit for discharging the cells and the culture solution from the cell culture flask, and a cell culture flask receiving part which is comprised of first and second vertical frames in which a plurality of injecting parts and collecting parts, and plate type connection parts which are respectively disposed between upper ends and lower ends of the two opposed vertical frames to be spaced apart from each other in regular intervals, and a mass automatic cell culture device having it.
    Type: Grant
    Filed: November 28, 2008
    Date of Patent: June 16, 2015
    Assignee: CORESTEM CO., LTD.
    Inventors: Kyung Suk Kim, Jai Jun Choung
  • Publication number: 20150079238
    Abstract: Edible microcarriers, including microcarrier beads, microspheres and microsponges, appropriate for use in a bioreactor to culture cells that may be used to form a comestible engineered meat product. For example, the edible microcarriers described herein may include porous microcarriers that may be used to grow cells (e.g., smooth muscle cells) and may be included with the cells in the final engineered meat product, without requiring modification or removal of the cells from the microcarriers. In a particular example, the edible microcarriers may be formed of cross-linked pectin, such as pectin-thiopropionylamide (PTP), and RGD-containing polypeptide, such as thiolated cardosin A. Methods of forming edible microcarriers, methods of using the edible microcarriers to make engineered meat, and engineered meat including the edible microcarriers are also described herein.
    Type: Application
    Filed: September 15, 2014
    Publication date: March 19, 2015
    Inventors: Francoise Suzanne MARGA, Brendan Patrick PURCELL, Gabor FORGACS, Andras FORGACS
  • Patent number: 8951255
    Abstract: A method of treating a spinal disk according to the present invention can include inserting an alloplastic bulking agent into the spinal disk to treat the defect. The alloplastic bulking agent has a plurality of microparticles. The bulking agent results in at least one of sealing the defect, increasing a pressure of the disk, increasing a height of the disk, improving stability of the disk and improving structural integrity of the disk.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: February 10, 2015
    Assignee: SpineOvations, Inc.
    Inventors: Neville Alleyne, Stuart Young
  • Patent number: 8906688
    Abstract: Cell expansion systems are provided. The cell expansion systems generally include a hollow fiber cell growth chamber, and first and second fluid flow paths associated with the interior of the hollow fibers and exterior of the hollow fibers, respectively. The hollow fibers have a hydrophilic interior surface and a hydrophobic exterior surface. Detachable flow circuits are also provided.
    Type: Grant
    Filed: April 14, 2008
    Date of Patent: December 9, 2014
    Assignee: Terumo BCT, Inc.
    Inventor: Glen Delbert Antwiler
  • Patent number: 8828720
    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 ?m and 400 ?m, such as about 200 ?m. It may have a cross sectional dimension of between 20 ?m and 30 ?m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 ?m and about 120 ?m, for example about 65 ?m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage.
    Type: Grant
    Filed: November 18, 2010
    Date of Patent: September 9, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Steve Oh, Shaul Reuveny, Allen Chen
  • Patent number: 8809054
    Abstract: The present invention is directed to a method to expand adherent cells comprising addition of adherent cells to an expansion container comprising microcarriers and culture medium; removing medium from the expansion container through a 8-20 mm filter; allowing cells to attach to microcarriers and keeping the expansion container in motion with an angle of between 30 to 90° and ?30 to ?90°. The present invention is also directed to a device suitable in the method. The advantage of the present invention is that fewer steps are needed to expand adherent cells, including stem cells like MSC opening the way for the use of autologeous and allogenous stem cell therapy. In addition, contamination risk is limited since the present invention may be carried out in a closed, disposable system.
    Type: Grant
    Filed: May 11, 2011
    Date of Patent: August 19, 2014
    Assignee: Xpand Biotechnology B.V.
    Inventors: Elise Leonore Isolde Dijkhuizen Borgart, Madelon Sophia George Maria Bracke, Joost Dick De Bruijn
  • Patent number: 8802430
    Abstract: A substrate for use in culturing cells or tissues. The substrate comprises a gel, one or more microstructures partially embedded within a surface of the gel, the one or more microstructures presenting two different curvatures or presenting two different stiffness values or presenting a combination of different curvatures and different stiffness values, wherein the microstructures are disposed at defined locations within the surface of the gel, and wherein the cells and tissues are cultured on an exposed surface of the microstructures.
    Type: Grant
    Filed: November 25, 2012
    Date of Patent: August 12, 2014
    Inventor: Shengyuan Yang
  • Patent number: 8716018
    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 ?m and 400 ?m, such as about 200 ?m. It may have a cross sectional dimension of between 20 ?m and 30 ?m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 ?m and about 120 ?m, for example about 65 ?m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage.
    Type: Grant
    Filed: March 17, 2009
    Date of Patent: May 6, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Steve Oh, Marti Lecina, Andre Choo, Shaul Reuveny, Robert Zweigert, Allen Chen
  • Patent number: 8691569
    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 ?m and 400 ?m, such as about 200 ?m. It may have a cross sectional dimension of between 20 ?m and 30 ?m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 ?m and about 120 ?m, for example about 65 ?m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage.
    Type: Grant
    Filed: November 1, 2010
    Date of Patent: April 8, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Steve Oh, Shaul Reuveny, Allen Chen
  • Patent number: 8637309
    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 ?m and 400 ?m, such as about 200 ?m. It may have a cross sectional dimension of between 20 ?m and 30 ?m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 ?m and about 120 ?m, for example about 65 ?m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage.
    Type: Grant
    Filed: August 16, 2011
    Date of Patent: January 28, 2014
    Assignee: Agency for Science, Technology and Research
    Inventors: Steve Oh, Marti Lecina, Andre Choo, Shaul Reuveny, Robert Zweigert, Allen Chen
  • Patent number: 8530415
    Abstract: The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible three-dimensional surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
    Type: Grant
    Filed: July 24, 2009
    Date of Patent: September 10, 2013
    Assignee: Histogen, Inc.
    Inventors: Gail K. Naughton, Frank Ziegler, Mark Baumgartner, Kyle Nickey
  • Patent number: 8524494
    Abstract: The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: September 3, 2013
    Assignee: Histogen, Inc.
    Inventors: Gail K. Naughton, Frank Ziegler, Mark Baumgartner, Kyle Nickey
  • Patent number: 8486699
    Abstract: Two cell lines, PICM-19H and PICM-19B, were derived from the bipotent ARS-PICM-19 pig liver stem cell line. The unipotent porcine stem cell line PICM-19H differentiates exclusively into hepatocytes and can be induced to express CYP450 enzymes. The growth rate and cell density in culture, morphological features, and hepatocyte detoxification functions, i.e., inducible CYP450 activity, ammonia clearance, and urea production of the PICM-19H cells were evaluated for their application in artificial liver devices. PICM-19H cells contain numerous mitochondria, Golgi apparatus, smooth and rough endoplasmic reticulum, vesicular bodies and occasional lipid vacuoles and display inducible CYP450 activity, clear ammonia, and produce urea in a glutamine-free medium. The data indicate that both cell lines, either together or alone, may be useful as the cellular substrate for an artificial liver device. The results demonstrate the potential for the use of PICM-19H cells in drug biotransformation and toxicity testing.
    Type: Grant
    Filed: May 23, 2008
    Date of Patent: July 16, 2013
    Assignee: The United States of America as Represented by the Secretary of Agriculture
    Inventors: Neil C. Talbot, Thomas J. Caperna, Ryan Willard
  • Patent number: 8415173
    Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.
    Type: Grant
    Filed: August 26, 2011
    Date of Patent: April 9, 2013
    Assignee: Crystal Bioscience Inc.
    Inventor: William Don Harriman
  • Patent number: 8404485
    Abstract: A coated microcarrier for cell culture includes a microcarrier base and a polymeric coating grafted to the base via a polymerization initiator. A method for forming the coated microcarrier includes (i) conjugating a polymerization initiator to the microcarrier base to form an initiator-conjugated microcarrier base; (ii) contacting the initiator-conjugated microcarrier base with monomers; and (iii) activating the initiator to initiate polymerization and graft the polymer to the base.
    Type: Grant
    Filed: July 25, 2012
    Date of Patent: March 26, 2013
    Assignee: Corning Incorporated
    Inventors: Kevin Robert McCarthy, Simon Kelly Shannon, Florence Verrier
  • Patent number: 8399253
    Abstract: A three-dimensional cell culture method for increasing cell proliferation efficiency by suitably regulating the proliferation-inducing and proliferation-inhibitory signals between cells is provided. The method includes repeatedly performing any one or both of the following processes a) and b) so as to regulate proliferation-inducing and proliferation-inhibitory signals between the cells: a) a process of gradually adding the micro-scaffolds, in which a small amount of the micro-scaffolds are used in an initial stage in order to maintain a suitable distance between the cells, and the amount of the micro-scaffolds is then increased slowly according to cell proliferation rate; and b) a periodic shaking process, in which shaking is performed in order to separate connected cells from each other by a physical force, after the cells are incubated for more than a given period of time.
    Type: Grant
    Filed: April 28, 2007
    Date of Patent: March 19, 2013
    Inventor: Hyunjin Yang
  • Patent number: 8398638
    Abstract: A method of treating a spinal disk according to the present invention can include inserting an alloplastic bulking agent into the spinal disk to treat the defect. The alloplastic bulking agent has a plurality of microparticles. The bulking agent results in at least one of sealing the defect, increasing a pressure of the disk, increasing a height of the disk, improving stability of the disk and improving structural integrity of the disk.
    Type: Grant
    Filed: August 30, 2005
    Date of Patent: March 19, 2013
    Assignee: SpineOvations, Inc.
    Inventors: Neville Alleyne, Stuart Young
  • Publication number: 20130059383
    Abstract: The present invention is directed to a method to expand adherent cells comprising addition of adherent cells to an expansion container comprising microcarriers and culture medium; removing medium from the expansion container through a 8-20 mm filter; allowing cells to attach to microcarriers and keeping the expansion container in motion with an angle of between 30 to 90° and ?30 to ?90°. The present invention is also directed to a device suitable in the method. The advantage of the present invention is that fewer steps are needed to expand adherent cells, including stem cells like MSC opening the way for the use of autologeous and allogenous stem cell therapy. In addition, contamination risk is limited since the present invention may be carried out in a closed, disposable system.
    Type: Application
    Filed: May 11, 2011
    Publication date: March 7, 2013
    Inventors: Elise Leonore Isolde Dijkhuizen Borgart, Madelon Sophia George Maria Bracke, Joost Dick De Bruijn
  • Patent number: 8318492
    Abstract: The present invention relates in one aspect to a method for determining the cell culture history of a cell unit labelled with more than one type of tag comprising the steps of: (a) measuring one or more parameters of each tag that is used to label the cell unit; (b) identifying each tag in the cell unit; and (c) correlating the identity of each tag to the identity of the cell unit and/or the specific cell culture conditions to which the cell unit has been exposed.
    Type: Grant
    Filed: February 25, 2008
    Date of Patent: November 27, 2012
    Assignee: Plasticell Limited
    Inventors: Yen Choo, Fraser Hornby, John Girdlestone
  • Patent number: 8216797
    Abstract: The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples.
    Type: Grant
    Filed: November 30, 2006
    Date of Patent: July 10, 2012
    Assignee: Massachusetts Institute of Technology
    Inventors: Eric Schwoebel, James Harper, Martha S. Petrovick, Frances Nargi, Mark Hollis, Bernadette Johnson, Joseph Lacirignola, Richard Mathews, Kristine Hogan, Trina Vian, Allan Heff, Mark Hennessy, Songeeta Palchaudhuri, Todd Rider
  • Patent number: 8202726
    Abstract: The present invention provides novel serum-free cell culture medium and methods for cultivating MDCK cells. In particular, non-tumorigenic MDCK cells. The present invention also provides methods for producing influenza viruses (e.g., particularly cold-adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.
    Type: Grant
    Filed: September 24, 2009
    Date of Patent: June 19, 2012
    Assignee: Medimmune, LLC
    Inventors: Jonathan Liu, Mark Thompson, Luis J. Maranga, Floro Cataniag, Simon S. Hsu
  • Patent number: 8148111
    Abstract: A carrier for cell culture is provided which improves the cell proliferativity in serum-free culture and which is free from risk from infection factor contamination. The gist of the features of the present invention is to be formed of a crosslinked poly(meth)acrylic acid (salt) particle (A) and an artificial polypeptide (P) having at least one cell-adhesive minimal amino acid sequence (X) in one molecule and to have a water retention value of from 2 to 50 g/g. The (A) is preferably a particle produced by reversed phase suspension polymerization of an aqueous monomer solution containing (meth)acrylic acid and/or an alkali metal salt of (meth)acrylic acid. The (P) preferably has at least one auxiliary amino acid sequence (Y) in one molecule of the (P). The (X) is preferably an Arg Gly Asp sequence.
    Type: Grant
    Filed: March 13, 2007
    Date of Patent: April 3, 2012
    Assignee: Sanyo Chemical Industries, Ltd.
    Inventors: Masato Kurokawa, Kazuhiro Takahashi
  • Patent number: 8119390
    Abstract: Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.
    Type: Grant
    Filed: March 6, 2009
    Date of Patent: February 21, 2012
    Assignee: New England Biolabs, Inc.
    Inventors: Christopher H. Taron, Paul A. Colussi
  • Patent number: 8097458
    Abstract: This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.
    Type: Grant
    Filed: April 20, 2010
    Date of Patent: January 17, 2012
    Assignee: Geron Corporation
    Inventors: Ramkumar Mandalam, Chunhui Xu, Joseph D. Gold, Melissa K. Carpenter
  • Patent number: 8093038
    Abstract: An apparatus and method for coating micron-sized or sub-micron-sized particles such as living cells. The coating apparatus includes an encapsulation chamber enclosing a two-layer water-oil system for coating each islet cell with an aqueous polymeric coat. Islets together with an aqueous polymer solution are fed by a feed device that utilizes the principle of hydrodynamic focusing in order to ensure encapsulation of individual islets. The polymer in the aqueous coat is subsequently crosslinked by being exposed to laser light to produce structurally stable microcapsules of controllable thickness of the order of tens of microns. Encapsulated islets are removed from the encapsulation chamber by a valveless pump and recovered by filtration or centrifugation.
    Type: Grant
    Filed: September 17, 2007
    Date of Patent: January 10, 2012
    Assignee: Illinois Institute of Technology
    Inventor: Dimitri T. Hatziavramidis
  • Patent number: 8030095
    Abstract: A gel microdrop composition is provided. In certain embodiments, the gel microdrop composition contains a polymer matrix, an effector particle that releases an effector molecule into the polymer matrix, a first reporter particle that emits a first optically detectable signal and a second reporter particle that emits a second optically detectable signal that is distinguishable from the first optically detectable signal, where the effector particle and said first and second reporter particles are encapsulated by the polymer matrix. Methods of screening that employ the gel microdrop composition and methods of making the gel microdrop composition are also disclosed.
    Type: Grant
    Filed: March 2, 2009
    Date of Patent: October 4, 2011
    Assignee: Crystal Bioscience Inc.
    Inventor: William Don Harriman
  • Publication number: 20110014693
    Abstract: We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 ?m and 400 ?m, such as about 200 ?m. It may have a cross sectional dimension of between 20 ?m and 30 ?m. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 ?m and about 120 ?m, for example about 65 ?m. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage.
    Type: Application
    Filed: March 17, 2009
    Publication date: January 20, 2011
    Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
    Inventors: Steve Oh, Marti Lecina, Andre Choo, Shaul Reuveny, Robert Zweigert, Allen Chen
  • Patent number: 7790456
    Abstract: The invention relates to a process for cultivating undifferentiated stem cells in suspension and in particular to a method for cultivating stem cells on microcarriers in vessels. The method enables stem cells to be cultivated in a scalable process for the first time, without losing their pluripotentiality. Different types of vessels are suitable for cultivation, such as e.g. spinners and bioreactors. The option of precisely setting the cultivation conditions in said vessels aids the process of obtaining a pluripotentiality of the stem cells. the inventive method also permits large yields of stem cells of an unvarying quality to be produced.
    Type: Grant
    Filed: September 9, 2005
    Date of Patent: September 7, 2010
    Assignee: Rheinische Friedrich-Wilhelms-Universitat
    Inventors: Stefanie Terstegge, Oliver Brüstle
  • Publication number: 20100178701
    Abstract: The present invention aims to provide a three-dimensional cell culture method for increasing cell proliferation efficiency by suitably regulating the proliferation-inducing and proliferation-inhibitory signals between cells, which are the greatest variables in proliferation efficiency.
    Type: Application
    Filed: April 28, 2007
    Publication date: July 15, 2010
    Inventor: Hyunjin Yang
  • Patent number: 7700355
    Abstract: A porous matrix, preparation thereof, and methods of using the same. The porous matrix of calcium alginate is prepared using a plurality of particles containing a multivalent cation, admixing the particles with ionic cross-linked polysaccharides and water to form a mixture, introducing a cross linker to solidify the mixture, dissolving the particles of the mixture in an acid to form a porous structure, and neutralizing and cross-linking the porous structure.
    Type: Grant
    Filed: January 7, 2005
    Date of Patent: April 20, 2010
    Assignee: Industrial Technology Research Institute
    Inventors: Chun-Jen Liao, Jui-Hsiang Chen, Chen-Chi Tsai, Yung-Chih Wu, Shu-Fang Chiang, Yi-Jung Hsiang
  • Publication number: 20090208919
    Abstract: Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.
    Type: Application
    Filed: January 16, 2008
    Publication date: August 20, 2009
    Applicant: Argylla Technologies, LLP
    Inventors: Joseph G. Utermohlen, Michael E. Hogan
  • Publication number: 20090136912
    Abstract: A carrier for cell culture is provided which improves the cell proliferativity in serum-free culture and which is free from risk from infection factor contamination. The gist of the features of the present invention is to be formed of a crosslinked poly (meth) acrylic acid (salt) particle (A) and an artificial polypeptide (P) having at least one cell-adhesive minimal amino acid sequence (X) in one molecule and to have a water retention value of from 2 to 50 g/g. The (A) is preferably a particle produced by reversed phase suspension polymerization of an aqueous monomer solution containing (meth)acrylic acid and/or an alkali metal salt of (meth)acrylic acid. The (P) preferably has at least one auxiliary amino acid sequence (Y) in one molecule of the (P). The (X) is preferably an Arg Gly Asp sequence.
    Type: Application
    Filed: March 13, 2007
    Publication date: May 28, 2009
    Applicant: SANYO CHEMICAL INDUSTRIES, LTD.
    Inventors: Masato Kurokawa, Kazuhiro Takahashi
  • Publication number: 20090093058
    Abstract: The subject of the present invention is to provide a microarray for introducing nucleic acid, the microarray capable of introducing and expressing nucleic acid into cells simply by adding the nucleic acid onto a plate and the like, and then seeding the cells thereon and culturing them without adding a nucleic acid-introducing reagent or additives. The subject is achieved by preparing the microarray including atelocollagen, a gene-introducing agent and nucleic acid on a plate and the like for the introduction of nucleic acid. The nucleic acid can be introduced into a cell by seeding cells into which nucleic acids are introduced on the microarray and culturing them without the need of preparing a mixture of viral vectors, nucleic acids and a nucleic acid-introducing agent after culturing cells or the need of adding a nucleic acid-introducing agent and additives.
    Type: Application
    Filed: July 22, 2005
    Publication date: April 9, 2009
    Inventors: Kimi Honma, Takahiro Ochiya
  • Publication number: 20090047260
    Abstract: Provided herein are cell culture substrates and microcarriers that include a keratin, e.g., in porous particulate form. The substrate may be provided in or further includes a liquid carrier and/or viable cells. The keratin may be alpha kerateines, gamma kerateines, and combinations thereof, and may be in the form of a meta keratin. In some embodiments, the keratin is acidic or basic. Methods of administering cultured cells are also provided, including administering the cell culture substrates or microcarriers to a subject in need thereof. Kits are further provided, and may include a suitable container; a plurality of cell culture substrates or microcarriers as described herein packaged into said container; and optionally, instructions for use.
    Type: Application
    Filed: August 15, 2008
    Publication date: February 19, 2009
    Applicant: Wake Forest University Health Sciences
    Inventor: Mark E. Van Dyke
  • Publication number: 20080176213
    Abstract: Provided is a method for controlling the degree of labeling (DOL) of a carrier molecule or solid support by the addition of a reactive label competitor to the labeling reaction. When the reactive label competitor is added to the labeling solution the competitor competes with the carrier molecule or solid support for the label, reducing the number of labels available to conjugates to the carrier molecule or solid support. This provides for a facile method that predictably alters the DOL of a carrier molecule or solid support.
    Type: Application
    Filed: March 27, 2008
    Publication date: July 24, 2008
    Applicant: INVITROGEN CORPORATION
    Inventors: John Matthew Mauro, Thomas Harry Steinberg, Lawrence I. Greenfield, Louis Leong
  • Patent number: 7312065
    Abstract: The present invention relates to Lawsonia intracellularis vaccines and methods for protecting against and diagnosing L. intracellularis infection. The products and processes of the invention are attainable, in part, as the result of an improved method for cultivating large scale supplies of L. intracellularis, including both a novel isolate of L. intracellularis of European origin and a method of preparing a lyophilized product containing the attenuated European isolate as vaccine product.
    Type: Grant
    Filed: July 15, 2004
    Date of Patent: December 25, 2007
    Assignee: Boehringer Ingelheim Vetmedica, Inc.
    Inventors: Michael B. Roof, Jeremy J. Kroll, Jeffrey P. Knittel
  • Patent number: 7198947
    Abstract: The present invention provides a method for production of functional proteins including hormones by renal cells in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel. Natural mixture of renal cells expresses the enzyme 1-a-hydroxylase which can be used to generate the active form of vitamin D: 1,25-diOH vitamin D3. The fibroblast cultures and co-culture of renal cortical cells express the gene for erythropoietin and secrete erythropoietin into the culture supernatant. Other shear stress response genes are also modulated by shear stress, such as toxin receptors megalin and cubulin (gp280). Also provided is a method of treating in-need individual with the functional proteins produced in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel.
    Type: Grant
    Filed: December 11, 2003
    Date of Patent: April 3, 2007
    Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
    Inventors: Thomas John Goodwin, Timothy Grant Hammond, James Howard Kaysen
  • Patent number: 7101719
    Abstract: Disclosed is a method for the measurement of a cellular process, or for the measurement of the effect of a test compound on a cellular process, in one or more different populations of cells. The method comprises providing separate samples of one or more different populations of cells adhering to support particles, the support particles comprising a scintillant substance and being adapted for cell growth. In one embodiment, different samples of cells are introduced into separate reaction vessels in a fluid medium, together with a reagent labelled with a radioisotope, in the presence or the absence of the test compound, under conditions so as to cause a portion of said radiolabelled reagent to become associated with the cells. In another embodiment, multiparameter analysis may be performed to determine the effect of a test compound on a cellular process using two or more different cell populations present in the same well.
    Type: Grant
    Filed: November 5, 2001
    Date of Patent: September 5, 2006
    Assignee: GE Healthcare Limited
    Inventors: Gerard Bernard O'Beirne, Rahman Aziz Ismail, Nicholas Thomas
  • Patent number: 7071005
    Abstract: A method and device are described to concentrate target organisms from a mixture of organisms. Beads (1) made of material such as nylon, polystyrene or glass are coated with antibodies of specific microorganisms. The beads (1) are contained in an enclosure (2) surrounded by grid material (4). The pore size of the grid is smaller than the size of the beads, to assure that the beads stay within the grid material and larger than the size of the microorganisms to allow the interaction of the microorganisms with the beads. A rod (5) is attached to the upper part of the enclosure (2) allowing the agitation of the device inside he growth medium containing the target organisms.
    Type: Grant
    Filed: August 23, 1999
    Date of Patent: July 4, 2006
    Assignee: Centrus International, Inc.
    Inventor: Ruth F. Eden
  • Patent number: 7045363
    Abstract: A nucleic acid-bound polypeptide produced by binding a nucleic acid to a polypeptide, a method of producing the nucleic acid-bound polypeptide, and applications of the nucleic acid-bound polypeptide, including immunoassays for an antigen or antibody, such as an agglutination immunoassay are provided.
    Type: Grant
    Filed: May 7, 1999
    Date of Patent: May 16, 2006
    Assignee: Fujirebio Inc.
    Inventors: Fuminori Takemura, Eiichi Ueno, Satoru Itoh
  • Patent number: 6864084
    Abstract: A method for floating at least one substance for growing a tissue part in a bioreactor. The method includes providing at least one substance consisting of one of a tissue part, a scaffold having cells deposited thereon, and a scaffold including a tissue part thereon. The method further includes further acting upon the substance with fluid, wherein the fluid holds the substance in free floatation. The fluid flows in a direction counter to gravity when a density of the substance is greater than a density of the fluid, and in a direction counter to buoyancy when a density of the substance is less than a density of the fluid. The bioreactor for floating at least one substance for growing a tissue part includes a container including a first flow chamber and at least one substance consisting of one of a tissue part, a scaffold having cells deposited thereon, and a scaffold including a tissue part thereon. The substance is acted upon with fluid. The bioreactor further includes an apparatus for conveying the fluid.
    Type: Grant
    Filed: October 31, 2001
    Date of Patent: March 8, 2005
    Assignee: Levitronix LLC
    Inventor: Reto Schöb
  • Patent number: 6858146
    Abstract: Artificial liver devices and methods for using the devices to purify a biological fluid are disclosed. The methods include the use of living hepatocytes (23) which are either unattached or attached to inert carriers and suspended in a cell culture medium which circulates in the devices with the hepatocytes (23). Blood or plasma passes on one side (7?) of semi-permeable membranes, on the other side (7) of which is the cell culture medium and across which is a concentration and/or pressure gradient. Solutes diffusing across the membrane into the cell culture medium are metabolized by the hepatocytes (23) and/or captured by additional removal means (4). Those undesirable substances which do not diffuse out of the blood or plasma into the hepatocyte containing culture medium are captured by additional removal means (50).
    Type: Grant
    Filed: September 27, 1994
    Date of Patent: February 22, 2005
    Assignee: Xenogenics
    Inventors: Edward F. Myers, Albert P. Li, Achilles A. Demetriou
  • Publication number: 20040170964
    Abstract: The present invention is directed to a composition and method for regulating the adhesion of cells and biomolecules to hydrophobic surfaces and hydrophobic coated surfaces. The composition is a biomolecule conjugated end-group activated polymer (EGAP). The biomolecule conjugated EGAP can be put to numerous uses including cell adhesion, cell growth, cell sorting, and other biological assays.
    Type: Application
    Filed: December 30, 2003
    Publication date: September 2, 2004
    Inventors: Karin D. Caldwell, Patrick A. Tresco, Jennifer Neff
  • Patent number: 6783983
    Abstract: This invention relates to methods for the cultivating cells, and in particular to methods for propagating viruses.
    Type: Grant
    Filed: October 5, 2000
    Date of Patent: August 31, 2004
    Assignee: Schering Corporation
    Inventors: Russell G. G. Condon, Nancy V. Connelly, Andreas Frei, Edward Glowacki, Vijay Yabannavar, Serge Batandolo
  • Patent number: 6737074
    Abstract: The present invention provides fibrin microbeads that are biologically active and comprise extensively cross-linked fibrin(ogen), and a method for preparing the fibrin microbeads. The present invention also provides a composition comprising cells bound to the fibrin microbeads, and methods for culturing and separating cells using the fibrin microbeads of the present invention. Finally, the present invention provides methods for transplanting cells and engineering tissue using the fibrin microbeads of the present invention.
    Type: Grant
    Filed: October 25, 2002
    Date of Patent: May 18, 2004
    Assignee: Hadasit Medical Research & Development Ltd.
    Inventors: Gerard Marx, Raphael Gorodetsky
  • Patent number: 6730498
    Abstract: The present invention provides a method for production of functional proteins including hormones by renal cells in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel. Natural mixture of renal cells expresses the enzyme 1-a-hydroxylase which can be used to generate the active form of vitamin D: 1,25-diOH vitamin D3. The fibroblast cultures and co-culture of renal cortical cells express the gene for erythropoietin and secrete erythropoietin into the culture supernatant. Other shear stress response genes are also modulated by shear stress, such as toxin receptors megalin and cubulin (gp280). Also provided is a method of treating in-need individual with the functional proteins produced in a three dimensional co-culture process responsive to shear stress using a rotating wall vessel.
    Type: Grant
    Filed: April 7, 1998
    Date of Patent: May 4, 2004
    Assignee: The United States of America as represented by the Administrator of the National Aeronautics and Space Administration
    Inventors: Thomas John Goodwin, Timothy Grant Hammond, James Howard Kaysen
  • Patent number: 6688041
    Abstract: The present invention relates to the use of a pigment form or pigment concentrate comprising of an organic pigment, and optionally an inorganic filler and/or an organic additive for coloring of seeds. Seeds colored by the dry pigment forms or pigment concentrates show an homogeneous color and in some cases growth promotion effects.
    Type: Grant
    Filed: March 7, 2001
    Date of Patent: February 10, 2004
    Assignee: Ciba Specialty Chemicals Corporation
    Inventors: Fridolin Bäbler, Kenneth Schumann
  • Publication number: 20040002558
    Abstract: Described are osteogenic paste compositions with enhanced osteoinductive properties for use in bone repair. Compositions comprising a quickly resorbable paste carrier, a more slowly resorbed mineral matrix, and Bone Morphogenetic Protein (BMP) or other osteogenic factor are described which enable increased osteoinductive activity while retaining a reliable scaffold for the formation of new bone at the implant site. Methods for making and methods for therapeutic use of the compositions are also disclosed.
    Type: Application
    Filed: August 6, 2001
    Publication date: January 1, 2004
    Inventor: William F. McKay
  • Patent number: 6660509
    Abstract: Abstract of the Invention The present invention comprises novel culture methods and devices in which living cells or subcellular biocatalysts are immobilized by the opposition of forces. The immobilized cells or biocatalysts may be attached to support complexes that add to the resultant vector forces. One embodiment of the apparatus comprises a cruciform design. Commercial applications of the apparatus include efficient isolation of metals from ores and removal of gases.
    Type: Grant
    Filed: May 21, 1999
    Date of Patent: December 9, 2003
    Assignee: Kinetic Biosystems, Inc.
    Inventors: Heath H. Herman, Tod M. Herman, Arlan K. Andrews, Sr.
  • Patent number: 6632658
    Abstract: The method for the holding in flotation of a substance such as a tissue part in a bioreactor (61) is characterized in that the substance (73) is acted upon with a fluid; and in that the flow of the fluid acts counter to gravity in such a manner that the substance (73) is held in flotation. The bioreactor (61) with a container (62) for a substance (73) which is to be acted upon with fluid comprises a first flow chamber (66a) to which a flowing fluid can be supplied, with the first flow chamber (66a) being designed in such a manner that the fluid which flows upwardly therein has a lower speed with increasing height.
    Type: Grant
    Filed: September 5, 2000
    Date of Patent: October 14, 2003
    Assignee: Levitronix LLC
    Inventor: Reto Schoeb