Culture Medium, Per Se Patents (Class 435/404)
  • Patent number: 10400007
    Abstract: A method for purifying a biologically active substance from a solution mixture containing impurities and the biologically active substance, in which an ion exchange chromatography carrier comprising a matrix and a copolymer containing at least N-isopropylacrylamide as a monomer unit and immobilized to a surface of the matrix is used, and the solution mixture is allowed to flow through a container storing the carrier at a uniform temperature, thereby recovering the biologically active substance.
    Type: Grant
    Filed: April 15, 2014
    Date of Patent: September 3, 2019
    Assignee: ASAHI KASEI MEDICAL CO., LTD.
    Inventors: Hiroki Taniguchi, Masako Goto, Ichiro Koguma, Yoshiro Yokoyama
  • Patent number: 10138467
    Abstract: The present invention relates to new serum- and protein-free culture media. These media are high performance culture media, which notably improve mammalian fed-batch cultures. The present invention also relates to methods for preparing and/or designing the medium, and methods of use thereof.
    Type: Grant
    Filed: April 3, 2014
    Date of Patent: November 27, 2018
    Assignee: ARES TRADING S.A.
    Inventors: Herve Broly, Matthieu Stettler, Martin Jordan, Arnaud Perilleux, Yolande Rouiller
  • Patent number: 10131891
    Abstract: The present invention relates to a method for controlling a glycosylation pattern of a recombinant glycoprotein, comprising culturing a cell comprising polynucleotide encoding a recombinant glycoprotein in a culture medium comprising insulin.
    Type: Grant
    Filed: December 30, 2015
    Date of Patent: November 20, 2018
    Assignee: LG CHEM, LTD.
    Inventors: Kyu Yong Kim, Soo Hyun Ryu, Sam Sook Seol, Sun Young Kim
  • Patent number: 10119915
    Abstract: A detection device for specimens includes an image sensor, a light-guiding structure, a carrier, and a light source. The light-guiding structure is disposed on the image sensor, and includes a light-guiding layer and a top layer. The light-guiding layer is disposed on the image sensor. The top layer is disposed on the light-guiding layer. The carrier is disposed on the light-guiding structure. The carrier has a number of wells arranged in an array located over the guiding portions. Each of the wells is configured to receive a specimen.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: November 6, 2018
    Assignee: VISERA TECHNOLOGIES COMPANY LIMITED
    Inventors: Zong-Ru Tu, Chin-Chuan Hsieh, Wei-Ko Wang
  • Patent number: 10111985
    Abstract: Provided herein are biocompatible hydrogel polymers capable of gelling in vivo comprising a therapeutic agent such as a protein or other biomolecule and kits comprising at least one nucleophilic compound or monomer unit, at least one electrophilic compound or monomer unit, and at least one therapeutic agent. The biocompatible hydrogel polymer is bioabsorbable and releases the therapeutic agent at a target site, avoiding systemic exposure and achieving a controlled delivery.
    Type: Grant
    Filed: August 9, 2012
    Date of Patent: October 30, 2018
    Assignee: MEDICUS BIOSCIENCES, LLC
    Inventors: Syed H. Askari, Yeon S. Choi, George Horng
  • Patent number: 10066000
    Abstract: High-Yield mammalian transient expression systems can include a cell culture media (particularly serum free, non animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.
    Type: Grant
    Filed: May 2, 2013
    Date of Patent: September 4, 2018
    Assignee: Life Technologies Corporation
    Inventors: Sanjay Vasu, Henry Chiou, Jeffrey Rogers, Maria Isabel Cisneros, Jingqiu Li, Chao Yan Liu, Meredith Jones
  • Patent number: 10034968
    Abstract: Compositions and methods are provided for preparing an adhesion barrier in the form of a foldable or flexible biodegradable polymer matrix that effectively reduces, prevents or treats adhesions in a patient in need thereof. In one embodiment, the matrix comprises a first porous layer comprising collagen and a second porous layer comprising collagen and dextran, wherein the dextran is loaded in the second layer in an amount of from about 5% to about 90% by weight based on a total weight of the matrix. In some embodiments, the matrix prevents or reduces cell growth into the matrix so as to reduce or prevent adhesions. In some embodiments, the matrix is in a sheet or strip form that can be folded into a tube form.
    Type: Grant
    Filed: February 8, 2017
    Date of Patent: July 31, 2018
    Assignee: Warsaw Orthopedic, Inc.
    Inventor: Scott M. Vickers
  • Patent number: 9856455
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury.
    Type: Grant
    Filed: November 23, 2015
    Date of Patent: January 2, 2018
    Assignee: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Patent number: 9550969
    Abstract: An inflatable bioreactor bag for cell cultivation, which comprising a top and a bottom sheet of flexible material, joined together to form two end edges and two side edges, wherein one baffle or a plurality of baffles extend from the bottom sheet in a region where the shortest distance to any one of the two end edges is higher than about one fourth of the shortest distance between the two end edges.
    Type: Grant
    Filed: March 16, 2012
    Date of Patent: January 24, 2017
    Assignee: GE Healthcare Bio-Sciences AB
    Inventors: Veronique Chotteau, Rafael Diana, Christian Kaisermayer, Eva Lindskog, Craig Robinson, Jimmie L. Rucker, Kieron D. Walsh
  • Patent number: 9481861
    Abstract: In embodiments there are disclosed culture media, compositions for supplementing culture media, and methods for culturing biological samples. In embodiments the methods are methods for detecting bacteria and in embodiments the bacteria include Salmonella spp and in embodiments include E. coli spp. In embodiments the media and compositions comprise an alkanolamine and a cobalamin compound.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: November 1, 2016
    Assignee: Foodchek Systems, Inc.
    Inventors: Gabriela Martinez, Renaud Tremblay, Janikim Larochelle
  • Patent number: 9428727
    Abstract: The present application describes an optimized medium for growth of mammalian cells as well as polypeptide production. The cell culture medium is characterized by a Sow ratio of sodium to potassium ions, it further relates to the method of producing polypeptides using such cell culture media. Sn another aspect, the method of polypeptide production can also comprise a temperature shift and/or a pH-shift to further optimize growth and product yield.
    Type: Grant
    Filed: April 25, 2011
    Date of Patent: August 30, 2016
    Assignee: Novartis AG
    Inventors: Christian Leist, Petra Meissner, Jörg Schmidt
  • Patent number: 9359417
    Abstract: A cell culture media for plasmid production is provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided herein is a method of making a cell culture media for plasmid production and a method for growing a plasmid vector. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality.
    Type: Grant
    Filed: January 25, 2013
    Date of Patent: June 7, 2016
    Assignee: Warsaw Orthopedic, Inc.
    Inventors: David S. Scher, Roger E. Harrington
  • Patent number: 9340814
    Abstract: A method for producing a recombinant protein includes steps of: (a) culturing a transformed cell in a protein-free and lipid-free medium containing no exogenous growth factors, in which the transformed cell is produced by transfecting a cell of a cell line derived from Chinese Hamster Ovary (CHO) cells, the cell line is adapted to a protein-free and lipid-free medium, and the cell is capable of proliferating in a suspended state in a protein-free and lipid-free medium containing no exogenous growth factors, with a vector containing a gene coding for the protein to be produced under the control of a promoter operable in the cell, and (b) recovering the protein produced by the transformed cell.
    Type: Grant
    Filed: February 21, 2014
    Date of Patent: May 17, 2016
    Assignees: Kyokuto Pharmaceutical Industrial Co., Ltd., National Institute for Materials Science
    Inventors: Tetsuji Sasaki, Akiyoshi Taniguchi
  • Patent number: 9192632
    Abstract: Methods of producing stem cell conditioned media to treat mammalian injuries or insults. In at least one embodiment of a method of producing a stem cell conditioned media of the present disclosure, the method comprises the steps of culturing at least one stem cell in a first cell culture medium, replacing some or all of the first cell culture medium with a second cell culture medium and further culturing the at least one stem cell in the second cell culture medium, and collecting a quantity of the second cell culture medium after a culture duration, wherein the quantity of the second cell culture medium contains a cell culture byproduct effective to treat a mammalian insult or injury. In another embodiment, the step of culturing comprises culturing the at least one stem cell in EGM2MV.
    Type: Grant
    Filed: May 22, 2012
    Date of Patent: November 24, 2015
    Assignee: NeuroFx, Inc.
    Inventors: Keith Leonard March, Brian H. Johnstone, Yansheng Du
  • Patent number: 9074176
    Abstract: Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.
    Type: Grant
    Filed: January 11, 2007
    Date of Patent: July 7, 2015
    Assignee: Two Cells Co., Ltd.
    Inventors: Yukio Kato, Jin Chang Shao, Yuki Katsura, Koichiro Tsuji
  • Publication number: 20150140543
    Abstract: Disclosed are compositions and methods for the preservation, storage, and transport of living biological tissues, organs, and populations of isolated cells. In particular, the disclosed compositions and processes permit mammalian cells, tissues, and organs to be harvested from suitable donor animals, stored for prolonged periods, and transported to the site of recipient implantation, all without significant loss of cell viability, biological activity, and/or tissue integrity.
    Type: Application
    Filed: December 24, 2014
    Publication date: May 21, 2015
    Inventors: Daniel Andrew Shimko, Scott P. Noel, Susan J. Drapeau, Josee Roy
  • Patent number: 9029146
    Abstract: We disclose a method of preparing a conditioned cell culture medium, the method comprising the steps of: (a) culturing a mesenchymal stem cell (MSC), a descendent thereof or a cell line derived therefrom in a cell culture medium; and (b) optionally isolating the cell culture medium; in which the mesenchymal stem cell (MSC) is obtained by propagating a cell obtained by dispersing a embryonic stem (ES) cell colony, or a descendent thereof, in the absence of co-culture in a serum free medium comprising FGF2.
    Type: Grant
    Filed: August 15, 2007
    Date of Patent: May 12, 2015
    Assignee: Agency for Science, Technology and Research
    Inventors: Sai Kiang Lim, Elias Lye
  • Publication number: 20150118709
    Abstract: The present invention provides a corn active peptide additive for cell culture medium, wherein in the corn active peptide additive, oligopeptides with molecular weight of lower than 1000 Dalton account for equal to or more than 90 wt % of total proteins, and the oligopeptides at least comprise one or more of AP, SAP, PAL, VNAP, PSSQ, and TQPGPQ. The corn active peptide additive of the present invention can be compounded with various basic culture mediums for serum-free culture of various animal cells, which not only substantially lowers the cost for cell culturing and reduces pollution and other problems caused by an animal derived component, but also can promote cell proliferation, improve cell viability and enhance expression of cell products.
    Type: Application
    Filed: December 24, 2014
    Publication date: April 30, 2015
    Inventors: MUYI CAI, FENG LIN, YAN LIU, HAIXIN ZHANG, RUIZENG GU, JUN LU, YING WEI
  • Patent number: 9018010
    Abstract: Provided are serum-free culture media which comprise basic fibroblast growth factor (bFGF), transforming growth factor beta-3, ascorbic acid, xeno-free serum replacement and a lipid mixture. The media may also comprise an IL6R/IL6 chimera, or leukemia inhibitory factor (LIF); wherein the culture medium is capable of maintaining pluripotent stem cells in an undifferentiated state in the absence of feeder cell support. Also provided are cell cultures comprising pluripotent stem cells such as human embryonic stem cells and induced pluripotent stem (iPS) cells, and methods of using the same for expanding pluripotent stem cells in an undifferentiated state using two-dimensional or three-dimensional culture systems. Methods of expanding iPS cells in a suspension culture devoid of substrate adherence and cell encapsulation are also provided.
    Type: Grant
    Filed: November 11, 2010
    Date of Patent: April 28, 2015
    Assignee: Technion Research & Development Foundation Limited
    Inventors: Michal Amit, Joseph Itskovitz-Eldor
  • Publication number: 20150104864
    Abstract: A method is described for generating a novel cancer stem cell line that possesses characteristics associated with stem cells, by co-culturing a human immortalized cell line and bone marrow-derived mesenchymal stem cells, and the novel cancer stem cell line established thereby. This method is able to readily generate cancer stem cells that are low in the level of structural chromosomal aberrations and are excellent in oncogenicity, and is effectively applicable to the development of anti-cancer drugs and personalized drugs.
    Type: Application
    Filed: March 24, 2014
    Publication date: April 16, 2015
    Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION
    Inventors: Sunghoi Hong, Seung-Ick Oh, Hang-Soo Park
  • Patent number: 9005971
    Abstract: A container system includes a bag having of one or more sheets of flexible polymeric material, the bag having an interior surface at least partially bounding a chamber that is adapted to hold a fluid. A sparger is secured to the interior surface of the bag so that a compartment is formed between the interior surface of the bag and the sparger. At least a portion of the sparger includes a sparging sheet formed from a flexible sheet of a gas permeable material. A tubular port or tube is coupled with the bag so that a passage bounded by the tubular port or tube communicates with the compartment.
    Type: Grant
    Filed: December 2, 2013
    Date of Patent: April 14, 2015
    Assignee: Life Technologies Corporation
    Inventors: Michael E. Goodwin, Nephi D. Jones, Jeremy K. Larsen
  • Publication number: 20150099301
    Abstract: The present invention relates to a cell culture medium and more particularly, to a safe, stable, and effective cell culture medium for cell therapy products.
    Type: Application
    Filed: February 28, 2013
    Publication date: April 9, 2015
    Inventors: Jeong Ik Lee, Soo Jung Lee, Kyung Mi Lee
  • Patent number: 8999916
    Abstract: The present invention relates to hydrogels comprising a plurality of amphiphilic peptides and/or peptoids capable of self-assembling into three-dimensional macromolecular nanofibrous networks, which entrap water and form said hydrogels, wherein at least a portion of said plurality of amphiphilic peptides and/or peptoids is chemically cross-linked. The present invention further relates to methods for preparing such hydrogels and to various uses of such hydrogels, e.g. as cell culture substrates, for drug and gene delivery, as wound dressing, as an implant, as an injectable agent that gels in situ, in pharmaceutical or cosmetic compositions, in regenerative medicine, in tissue engineering and tissue regeneration, or in electronic devices. It also relates to a method of tissue regeneration or tissue replacement using a hydrogel in accordance with the present invention.
    Type: Grant
    Filed: January 28, 2013
    Date of Patent: April 7, 2015
    Assignee: Agency for Science, Technology and Research
    Inventors: Charlotte Hauser, Wei Yang Seow
  • Patent number: 8993323
    Abstract: The present invention is directed to a method of deriving pluripotent embryonic stem cells from mouse blastocysts or from primordial germ cells from a post-implantation mouse embryo, or of maintaining or growing pluripotent embryonic stem cells from a mouse, or of expanding human hematopoietic stem cells or human hematopoietic precursor cells. The methods include the step of cultivating the stem cells or precursor cells for at least one passage in a culture medium preconditioned by the rabbit fibroblast cell line Rab9 (ATCC catalogue CRL1414) and containing less than 0.1 ng/ml Leukemia Inhibitory Factor (LIF).
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: March 31, 2015
    Assignee: ThromboGenics N.V.
    Inventor: Luc Schoonjans
  • Publication number: 20150087058
    Abstract: The present invention relates to a stem cell culture medium which can be substituted for a conventional stem cell culture medium containing the heterologous protein fetal bovine serum, and more particularly to a stem cell culture medium containing a basal medium and a knockout serum replacement and a method of culturing stem cells using the same. According to the invention, a high purity of stem cells having a reduced ability to spontaneously differentiate can be obtained without having to use the heterologous protein fetal bovine serum and expensive growth factors (EGF and bFGF), and thus the efficacy of stem cell therapy can be significantly increased.
    Type: Application
    Filed: April 17, 2013
    Publication date: March 26, 2015
    Applicant: SAMSUNG LIFE PUBLIC WELFARE FOUNDATION
    Inventor: Do Hyun Nam
  • Publication number: 20150079050
    Abstract: The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.
    Type: Application
    Filed: March 14, 2013
    Publication date: March 19, 2015
    Inventors: Kazuhisa Shinmura, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
  • Publication number: 20150072415
    Abstract: A medium is described for the protein-free and serum-free cultivation of cells, especially mammalian cells, whereby the medium contains a proportion of soy hydrolysate.
    Type: Application
    Filed: March 31, 2014
    Publication date: March 12, 2015
    Applicant: Baxter Innovations GmbH
    Inventors: Manfred Reiter, Wolfgang Mundt, Friedrich Dorner, Leopold Grillberger, Artur Mitterer
  • Publication number: 20150064786
    Abstract: This invention demonstrates the formation of a novel polarized membrane lipid raft signaling module in neurons, in response to several diverse neurotoxic stimuli. This polarization occurs well before neurons commit to die, and is an early mechanism in death signaling. The formation of this signaling module is dependent on cholesterol for its formation and provides a mechanistic explanation for the protective effects of cholesterol depleting drugs in several non-neural models of cell death. As such, the formation of the signaling module lends itself as a novel screen for the identification of new drugs and therapeutics which would retard its formation and protect against neuronal injury and death.
    Type: Application
    Filed: October 3, 2014
    Publication date: March 5, 2015
    Inventor: Adil A. KHAN
  • Publication number: 20150064785
    Abstract: Compositions and methods are described for preparing media, feeds, and supplements. Such methods and medias may display increased stability of labile components and may use, for example, microsuspension and/or encapsulation technologies, chelation, and optionally, coating and/or mixing the labile compounds with anti-oxidants. The compositions may withstand thermal and/or irradiation treatment and have reduced virus number. These techniques may result in product with extended shelf-life, extended release of their internal components into culture, or in product that can be added aseptically into a bioreactor using minimal volumes. The compositions and methods may optimize the bioproduction workflow and increase efficiency.
    Type: Application
    Filed: December 21, 2012
    Publication date: March 5, 2015
    Inventors: Richard Fike, Shawn Barrett, Zhou Jiang, David Judd
  • Publication number: 20150056702
    Abstract: A method is provided, including adding a phthalide to a stem cell medium to provide a phthalide-containing medium, and then culturing a stem cell using the phthalide-containing medium. The use of a phthalide in a medium for culturing stem cells optionally maintains the pluripotency of stem cells. The phthalide also enhances the generation efficiency of induced pluripotent stem cells to decrease the culture cost.
    Type: Application
    Filed: October 31, 2014
    Publication date: February 26, 2015
    Inventors: SHIH-PING LIU, SHINN-ZONG LIN, HORNG-JYH HARN, YING-JIUN CHIEN, CHIEN-YU HSU, CHENG-HSUAN CHANG
  • Patent number: 8962319
    Abstract: The present invention relates to methods for preparing an artificial immune system. The artificial immune system comprises a cell culture comprising T cells, B cells and antigen-primed dendritic cells. The artificial immune system of the present invention can be used for in vitro testing of vaccines, adjuvants, immunotherapy candidates, cosmetics, drugs, biologics and other chemicals.
    Type: Grant
    Filed: November 4, 2011
    Date of Patent: February 24, 2015
    Assignees: Sanofi Pasteur Vaxdesign Corp., Virginia Commonwealth University
    Inventors: William L. Warren, Donald Drake, III, Janice Moser, Inderpal Singh, Haifeng Song, Eric Mishkin, John G. Tew
  • Patent number: 8956830
    Abstract: Polypeptide preparations having target levels of glycans, and methods of producing such polypeptide preparations using DMSO, are described.
    Type: Grant
    Filed: March 14, 2013
    Date of Patent: February 17, 2015
    Assignee: Momenta Pharmaceuticals, Inc.
    Inventors: Holly Prentice, Rasheed Tijani, Brett Belongia
  • Publication number: 20150044178
    Abstract: The present invention provides a method for the normalized culturing of corneal endothelial cells. More specifically, the present invention provides a culture-normalizing-agent of a corneal endothelial cell, comprising a fibrosis inhibitor. In detail, the present invention provides a culture-normalizing agent comprising a transforming growth factor (TGF) ? signal inhibitor. The present invention also provides a culture medium for culturing a corneal endothelial cell normally, which comprises the culture-normalizing agent according to the present invention and corneal endothelium culture components. The present invention also provides a method for culturing a corneal endothelial cell normally, comprising the step of culturing a corneal endothelial cell using the culture-normalizing agent according to the present invention or the culture medium according to the present invention.
    Type: Application
    Filed: December 27, 2012
    Publication date: February 12, 2015
    Applicants: KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION, SENJU PHARMACEUTICAL CO., LTD., THE DOSHISHA
    Inventors: Shigeru Kinoshita, Noriko Koizumi, Naoki Okumura
  • Publication number: 20150044176
    Abstract: The invention provides improved methods for preparing hematopoietic cells for transplantation and the resulting improved hematopoietic cell compositions. The invention further relates to improved culture media and methods of culturing, processing, modulating, and expanding blood cell products for hematopoietic transplantation.
    Type: Application
    Filed: March 13, 2014
    Publication date: February 12, 2015
    Applicant: FATE THERAPEUTICS, INC.
    Inventor: Betsy Denise REZNER
  • Publication number: 20150037435
    Abstract: Provided are a culture medium of an adipose-derived stem cell, a method for preparing the same, and a composition for promoting hair growth, in which the composition includes the culture medium. The adipose-derived stem cell (ADSC-T) according to the present invention exhibits long lifespan, improved cell proliferation rate, and extended proliferation period, as compared with a primary adipose-derived stem cell (ADSC), and thus, the adipose-derived stem cell (ADSC-T) can be usefully used for the study about the adipose-derived stem cell and the mass production of the culture medium of the adipose-derived stem cell. In addition, according to the present invention, the culture medium of the adipose-derived stem cell (ADSC-T) that expresses a T antigen of SV40 exhibits excellent hair growth effectiveness and can be usefully used as a raw material for the hair loss prevention and hair growing agents.
    Type: Application
    Filed: August 29, 2012
    Publication date: February 5, 2015
    Inventors: Dong Wan Kim, Mi Jung Seo, Gwang Lee, Woo Hong Joo, Sun Hee Kim
  • Publication number: 20150030693
    Abstract: An object of the present invention is to provide an antitumor aqueous solution and an anticancer agent, both of which can kill cancer cells while having virtually no effects on normal cells, and to provide methods for producing the antitumor aqueous solution and the anticancer agent. The method of the invention for producing an antitumor aqueous solution for killing cancer cells includes an aqueous solution preparation step of preparing an aqueous solution through addition, to water, of a solute containing at least one of disodium hydrogen phosphate (Na2HPO4), sodium hydrogen carbonate (NaHCO3), L-glutamine, L-histidine, and L-tyrosine disodium dihydrate (L-tyrosine.2Na.2H2O); and a plasma irradiation step of irradiating the aqueous solution with atmospheric pressure plasma generated in a plasma generation region by means of a plasma generator.
    Type: Application
    Filed: February 26, 2013
    Publication date: January 29, 2015
    Applicants: c/o NATONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY, NU ECO ENGINEEERING CO., LTD.
    Inventors: Masaru Hori, Masaaki Mizuno, Fumitaka Kikkawa, Hiroaki Kajiyama, Fumi Utsumi, Kae Nakamura, Kenji Ishikawa, Keigo Takeda, Hiromasa Tanaka, Hiroyuki Kano
  • Publication number: 20150031128
    Abstract: The invention pertains to the use of (i) one or more C2-C6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof; and (ii) one or more compounds selected from—the group of amino acid derivatives consisting of ?-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine; -phenolic acid derivatives; -linear C2-C6 or cyclic C6 sugar alcohols; -pyridinic acid derivatives; and -nucleobases and/or nucleotides chosen from uracil, adenine, adenosine 3?-monophosphate (3?-AMP) and adenosine 5?-monophosphate (AMP), as a growth-and production promoting ingredient, in culture media for culturing eukaryotic cells. The invention further pertains to culture media containing these components at levels of at least 0.001 mg/l.
    Type: Application
    Filed: March 8, 2013
    Publication date: January 29, 2015
    Applicant: Friesland Brands B.V.
    Inventors: Abhishek Gupta, Mireille Maria Gadellaa, Dominick Yves Willy Maes
  • Publication number: 20150030570
    Abstract: Provided are a culture medium for preparing neural stem cell and use thereof, the culture medium for preparing neural stem cell comprising: a basic culture medium suitable for the growth of stem cell, and a cell signal pathway inhibitor selected from at least one of GSK inhibitor, MEK inhibitor, TGF-? inhibitor, ROCK inhibitor and BMP inhibitor.
    Type: Application
    Filed: February 6, 2013
    Publication date: January 29, 2015
    Applicant: Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences
    Inventors: Guangjin Pan, Duanqing Pei, Lihui Wang, Linli Wang, Yanting Xue
  • Patent number: 8940537
    Abstract: The present disclosure provides methods for maintaining and propagating undifferentiated pluripotent stem cells (SC) in suspension. The methods comprise culturing such SC in a non-adherent culture dish under conditions comprising a basic serum free medium and one or more of a basic medium, a serum replacement, an extra cellular matrix component and a factor supporting expansion of said SC. A specific and preferred culture condition comprise supplementing Neurobasal™ medium with KO serum replacement (KOSR). These conditions allowed for large scale and long term propagation of undifferentiated pluripotent SC. The culture system comprising suspended undifferentiated pluripotent SC were found to have many applications including in methods for directed as well as spontaneous differentiation of the SC into somatic cells. Also disclosed herein is a method of deriving SC, preferably human embryonic SC from human embryos via the formation of cell clusters.
    Type: Grant
    Filed: January 25, 2012
    Date of Patent: January 27, 2015
    Assignee: Hadasit Medical Research Services & Development Limited
    Inventors: Benjamin Reubinoff, Debora Steiner
  • Patent number: 8936807
    Abstract: Use of the antimicrobial cathelicidin peptide II-37, N-terminal fragments of LL-37 or extended sequences of LL-37 having 1-3 amino acids in the C-terminal end, for stimulating proliferation of epithelial and stromal cells and thereby healing of wounds, such as chronic ulcers. The cytotoxic effect of LL-37 may be reduced by including a bilayer-forming polar lipid, especially a digalactosyldiacylglycerol, in pharmaceutical compositions and growth media comprising LL-37.
    Type: Grant
    Filed: July 9, 2013
    Date of Patent: January 20, 2015
    Assignee: Lipopeptide AB
    Inventors: Mona Ståhle-Bäckdahl, Johan Heilborn, Anders Carlsson, Conny Bogentoft
  • Publication number: 20150017726
    Abstract: The present application discloses a cell culture media for growth, maintenance and induction of reversion to a less mature state of a cell comprising a MUC1* activating ligand.
    Type: Application
    Filed: April 16, 2014
    Publication date: January 15, 2015
    Applicant: Minerva Biotechnologies Corporation
    Inventors: Cynthia Bamdad, Benoit Smagghe
  • Publication number: 20150010898
    Abstract: Methods for preparation of culture media and production of recombinant proteins described herein.
    Type: Application
    Filed: October 26, 2012
    Publication date: January 8, 2015
    Applicant: Shire Human Genetic Therapies, Inc.
    Inventor: Robin Ng
  • Publication number: 20150011004
    Abstract: Embodiments described herein generally relate to systems and methods for promoting the expansion of high density non-adherent cells through the use of a cell growth chamber, a mass transfer device, and a fluid circulation loop. Improved cell growth is achieved in the cell growth chamber by using a chamber having a particular orientation and shape, e.g., conical, to create a media-rich reservoir for growing cells. By placing the chamber in a vertical position, the force of media flow along the chamber walls is substantially equal and opposite to the gravitational force on the cells. The interaction of these forces maintains the non-adherent cells in suspension. The use of the cell growth chamber in conjunction with the mass transfer device and fluid circulation loop(s) creates efficiencies by relying on the cumulative and combined features of the devices.
    Type: Application
    Filed: September 22, 2014
    Publication date: January 8, 2015
    Applicant: Terumo BCT, Inc.
    Inventor: Glen Delbert ANTWILER
  • Publication number: 20150011429
    Abstract: Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable freezing quality of the adipose-derived stem cells and influence of harmful factors in serum on the adipose-derived stem cells are solved, and the adipose-derived stem cells stored have the advantages of high survival percentage, well adherence growth and strong differentiation capacity.
    Type: Application
    Filed: August 6, 2012
    Publication date: January 8, 2015
    Applicants: CELLULAR BIOMEDICINE GROUP (WUXI) LTD, CELLULAR BIOMEDICINE GROUP (SHANGHAI) LTD
    Inventors: Helen Zhang, Luyi Zhang, Wei Cao
  • Patent number: 8916189
    Abstract: This invention is intended to provide cell aggregates that can reproduce functions of the myocardium, such as the function of beating, and thus are available for myocardial regenerative therapy, and to provide a cell culture support for producing the same. According to this invention, a cell culture support for forming string-shaped cardiomyocyte aggregates, which has a substrate portion having one surface on which at least one linear cell-adherent region is formed is provided.
    Type: Grant
    Filed: May 16, 2008
    Date of Patent: December 23, 2014
    Assignees: Dai Nippon Printing Co., Ltd., Tokyo Women's Medical University
    Inventors: Masanao Watanabe, Teruo Okano, Masayuki Yamato, Tatsuya Shimizu, Yoshikatsu Akiyama
  • Publication number: 20140370594
    Abstract: A method of growing a microorganism by culturing the microorganism in a an aqueous solution of carbohydrates containing C6-sugar monomers or C5-sugar monomers, wherein the aqueous solution of carbohydrates is made by reacting biomass or a biomass-derived reactant with a solvent system including a lactone and water, and an acid catalyst. The reaction yields a product mixture containing water-soluble C6-sugar-containing oligomers, C6-sugar monomers, C5-sugar-containing oligomers, C5-sugar monomers, or any combination thereof. The product mixture is then partitioned or extracted to yield an aqueous layer containing the carbohydrates and a substantially immiscible organic layer containing the lactone. The aqueous layer is used for growing the microorganisms.
    Type: Application
    Filed: June 14, 2013
    Publication date: December 18, 2014
    Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION
    Inventors: James A. Dumesic, Brian Pfleger, Jeremy S. Luterbacher, Jacqueline Rand
  • Publication number: 20140370600
    Abstract: Disclosed is a basic culture medium for mesenchymal stem cells, and a cell therapeutic agent cultured and differentiated using same. The basic culture medium reduces the time taken from collection to mass culturing by increasing the proliferation rate of undifferentiated mesenchymal stem cells derived from an adult tissue such as human marrow and adipose tissue, and also is capable of various differentiations into treating agents for bone-forming cells, for cartilage cells, or for fat cells.
    Type: Application
    Filed: September 6, 2011
    Publication date: December 18, 2014
    Applicant: SEWON CELLONTECH CO., LTD.
    Inventors: Dong-Sam Suh, Jun Keun Lee, Dong Il Chang, Min Jung Choi, Jang Hoon Kim, Ga Ram Kim, Cheong Ho Chang
  • Patent number: 8911964
    Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: December 16, 2014
    Inventors: Itzcoatl A. Pla, Joseph G. Matuck, John C. Fann, Christof Schulz, Nichole A. Roy, David F. Bruton, James McIntire, Yu-Hsiang David Chang, Thomas Seewoester
  • Patent number: 8906646
    Abstract: The invention describes improved methods and compositions for producing a recombinant protein, e.g., an antibody, in mammalian cell culture. In addition, the invention provides improved cell culture media, including improved production media, feed solutions, and combination feeds, which may be used to improve protein productivity in mammalian cell culture.
    Type: Grant
    Filed: March 26, 2014
    Date of Patent: December 9, 2014
    Assignee: AbbVie Inc.
    Inventors: Itzcoatl A. Pla, Joseph G. Matuck, John C. Fann, Christof Schulz, Nichole A. Roy, David F. Bruton, James McIntire, Yu-Hsiang David Chang, Thomas Seewoester
  • Publication number: 20140356336
    Abstract: The object of the invention is to provide a technique of suppressing tumorigenesis in IPS cells and inducing differentiation into target differentiated cells. In use of a statin and a differentiation inducer, iPS cells are differentiated into target differentiated cells, whereby iPS cells can be differentiated into differentiated cells in which tumorigenesis is suppressed.
    Type: Application
    Filed: December 27, 2012
    Publication date: December 4, 2014
    Inventor: Hiroshi Egusa