Contains An Animal Extract Patents (Class 435/408)
  • Publication number: 20150099301
    Abstract: The present invention relates to a cell culture medium and more particularly, to a safe, stable, and effective cell culture medium for cell therapy products.
    Type: Application
    Filed: February 28, 2013
    Publication date: April 9, 2015
    Inventors: Jeong Ik Lee, Soo Jung Lee, Kyung Mi Lee
  • Publication number: 20150064273
    Abstract: Mesenchymal stem cell conditioned medium and methods for preparing the conditioned medium are provided. The methods comprise culturing MSCs in a medium comprising nicotinamide or nicotinamide and fibroblast growth factor 4 (FGF4) and collecting the conditioned medium. Compositions comprising the mesenchymal stem cell conditioned medium and uses thereof are also provided.
    Type: Application
    Filed: February 13, 2013
    Publication date: March 5, 2015
    Inventors: Tony Peled, Yair Steinhardt
  • Patent number: 8932620
    Abstract: Methods of making a biologically active three-dimensional scaffold capable of supporting growth and differentiation of a cell are described. Biologically active three-dimensional scaffold made by the methods of the invention and an engineered tissue made from the scaffolds are described. Fibers of desired porosity can be obtained from non-structural ECM by lyophilization and/or electrospinning which can be useful for numerous tissue engineering applications requiring complex scaffolds, such as wound healing, artificial skin (burns), soft tissue replacement/repair and spinal cord injury.
    Type: Grant
    Filed: June 19, 2006
    Date of Patent: January 13, 2015
    Assignee: Drexel University
    Inventors: Peter I. Lelkes, Mengyan Li, Anat Perets, Honesto Poblete, Philip Lazarovici
  • Publication number: 20140370600
    Abstract: Disclosed is a basic culture medium for mesenchymal stem cells, and a cell therapeutic agent cultured and differentiated using same. The basic culture medium reduces the time taken from collection to mass culturing by increasing the proliferation rate of undifferentiated mesenchymal stem cells derived from an adult tissue such as human marrow and adipose tissue, and also is capable of various differentiations into treating agents for bone-forming cells, for cartilage cells, or for fat cells.
    Type: Application
    Filed: September 6, 2011
    Publication date: December 18, 2014
    Applicant: SEWON CELLONTECH CO., LTD.
    Inventors: Dong-Sam Suh, Jun Keun Lee, Dong Il Chang, Min Jung Choi, Jang Hoon Kim, Ga Ram Kim, Cheong Ho Chang
  • Patent number: 8911994
    Abstract: Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed.
    Type: Grant
    Filed: September 30, 2011
    Date of Patent: December 16, 2014
    Assignee: Artecel Sciences Inc.
    Inventors: Yuan-Di C. Halvorsen, William O. Wilkison, Jeffrey Martin Gimble
  • Publication number: 20140295555
    Abstract: A blood component such as platelets is concentrated. The concentrate, such as platelet-rich plasma, is used in a cell culture medium to grow and proliferate cells. The cells may be from the same person from which the blood concentrate is obtained. The cells grown in the culture medium may be used to treat a patient which may be the same patient from which the blood was extracted and/or the cells were obtained.
    Type: Application
    Filed: June 10, 2014
    Publication date: October 2, 2014
    Inventor: Allan Mishra
  • Publication number: 20140295554
    Abstract: The present invention provides a method for effectively extracting useful ingredients from an umbilical cord. The present invention provides an umbilical cord extract including the useful ingredients. The umbilical cord extract, according to the present invention, can be used as a serum substitute for cultivating ordinary cells and stem cells from an animal. Also, the umbilical cord extract, according to the present invention, can be used for a filler and a dressing for tissue restoration, and for a cosmetic composition for improving the skin. In addition, the present invention relates to a composition for a medium for separating and stem cells derived from tissue, such as an umbilical cord and fatty tissue, and to a method for separating and cultivating stem cells derived from the tissue using the same.
    Type: Application
    Filed: July 11, 2012
    Publication date: October 2, 2014
    Applicant: CHA BIO & DIOSTECH CO., LTD.
    Inventors: Sun Mi Kim, Youngjun Lee, Yong Soo Choi
  • Patent number: 8846400
    Abstract: A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith.
    Type: Grant
    Filed: November 26, 2012
    Date of Patent: September 30, 2014
    Assignee: Technion Research & Development Foundation Limited
    Inventors: Michal Amit, Joseph Itskovitz-Eldor
  • Patent number: 8796025
    Abstract: The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.
    Type: Grant
    Filed: May 8, 2012
    Date of Patent: August 5, 2014
    Assignee: Stemnion, Inc.
    Inventors: Charlotte A Emig, Catherine J Trumpower, Vivienne S Marshall
  • Patent number: 8771684
    Abstract: A method of alleviating pain associated with tissue damage includes applying salmon thrombin at a tissue damage site, as a single substance in liquid form, or as a powder, a foam, and/or a gel that includes salmon thrombin. A pain relief substance includes a salmon thrombin preparation.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: July 8, 2014
    Assignee: Sea Run Holdings, Inc.
    Inventors: Evelyn S. Sawyer, Paul A. Janmey, Beth A. Winkelstein
  • Patent number: 8741646
    Abstract: The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies.
    Type: Grant
    Filed: October 6, 2011
    Date of Patent: June 3, 2014
    Assignee: Stemnion, Inc.
    Inventors: Charlotte A Emig, Vivienne S Marshall
  • Publication number: 20140127314
    Abstract: In certain embodiments, this disclosure describes compositions comprising platelet lysates depleted of fibrinogen. In a further embodiment, the composition further comprises a cell culture medium component. This disclosure also provides a method for preparing the composition, comprising the steps of (a) lysing platelets providing a lysate; (b) removing cell debris; and (c) depleting fibrinogen by forming a removable mass by adding a metal salt such as calcium chloride. Furthermore, the disclosure also describes the product produced using said method.
    Type: Application
    Filed: June 26, 2012
    Publication date: May 8, 2014
    Applicants: CHILDREN'S HEATLHCARE OF ATLANTA, INC., EMORY UNIVERSITY
    Inventors: Ian B. Copland, Jacques Galipeau
  • Publication number: 20140115728
    Abstract: The invention provides double knockout transgenic pigs (GT/CMAH-KO pigs) lacking expression of any functional ?GAL and CMAH. Double knockout GT/CMAH-KO transgenic organs, tissues and cells are also provided. Methods of making and using the GT/CMAH-KO pigs and tissue are also provided.
    Type: Application
    Filed: March 14, 2013
    Publication date: April 24, 2014
    Inventor: A. Joseph Tector
  • Publication number: 20140106448
    Abstract: Provided herein are methods of isolating and expanding a plurality of multipotent cells (e.g., MSCs and ASCs) using a first culture in a suitable 2-dimensional substrate and a second culture in a suitable 3-dimensional substrate containing the basement membrane component and ROCK inhibitors. Also described are multipotent cell cultures made by the methods. Multipotent cell cultures as described may be used for transplantation or as niche cells for supporting other types of stem cells.
    Type: Application
    Filed: April 30, 2012
    Publication date: April 17, 2014
    Applicant: TissueTech, Inc.
    Inventors: Szu-yu Chen, Scheffer Tseng
  • Patent number: 8679838
    Abstract: It is intended to provide a serum which contains a large amount of growth factors capable of efficiently promoting the growth of stem cells. A human serum for cell culture which shows a residual ratio of platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%.
    Type: Grant
    Filed: February 12, 2010
    Date of Patent: March 25, 2014
    Assignee: JMS Co., Ltd.
    Inventors: Koji Suzuki, Seishin Tanaka
  • Publication number: 20140073575
    Abstract: Prolyl and lysyl hydroxylases isolated from Mimivirus are described. These are able to hydroxylate collagen. Isolated nucleic acids coding for the mentioned hydroxylases are incorporated into suitable vectors and used to express these hydroxylases in host cells, e.g. E. coli. Furthermore a method of manufacturing hydroxylated collagen in a host cell is described. The hydroxylases and the recombinantly expressed hydroxylated collagen are useful in clinical settings and biotechnology applications.
    Type: Application
    Filed: March 21, 2012
    Publication date: March 13, 2014
    Applicant: UNIVERSITAET ZUERICH PROREKTORAT MNW
    Inventors: Thierry Hennet, Christoph Rutschmann, Kelvin Luther
  • Publication number: 20140065173
    Abstract: Dendritic cells containing tumor lysate loaded particles are prepared. The dendritic cells present tumor antigens to elicit the Major Histocompatibility Complex class I pathway and can be used as a vaccine to treat cancer, including ocular melanoma.
    Type: Application
    Filed: September 5, 2013
    Publication date: March 6, 2014
    Applicant: Orbis Health Solutions LLC
    Inventor: Thomas E. Wagner
  • Patent number: 8617887
    Abstract: The present invention is generally in the field of neurological diseases and disorders, particular in the field of neurodegenerative diseases in which the myelin cover of nerves is lost. IL6R/IL6 chimera is used to promote the formation of oligodendrocytes from embryonic stem cells for treatment of neurodegenerative diseases or posttraumatic nerve damage.
    Type: Grant
    Filed: June 13, 2004
    Date of Patent: December 31, 2013
    Assignee: Yeda Research and Development Co. Ltd
    Inventors: Michel Revel, Peter Lonai, Rozemari Stirbu Lonai
  • Patent number: 8617893
    Abstract: The present invention discloses a composition that contains (1) an effective amount of an analgesically and/or anti-inflammatory active fraction separated from a mixture of plasma and/or serum, and (2) at least one metal, metal ion or metal salt, in which the mixture has been denatured. Also disclosed are methods of producing the composition for treating a subject afflicted with inflammation and/or pain.
    Type: Grant
    Filed: October 22, 2012
    Date of Patent: December 31, 2013
    Assignee: Cambridge Scientific Pty Ltd
    Inventors: Jeffrey D. Edwards, John Palermo
  • Patent number: 8603821
    Abstract: A method for preparing serum and a serum preparation apparatus is provided that can give a large amount of serum with high culture efficiency regardless of freshness of blood used. In a method for preparing serum from blood containing at least platelets, a platelet processing step is provided in which platelet membrane in the blood is destroyed. After the platelet processing step, a deposition step for depositing thermolabile protein in blood and a removal step for removing the thermolabile protein, which has been deposited in the deposition step, are preferably provided.
    Type: Grant
    Filed: March 5, 2008
    Date of Patent: December 10, 2013
    Assignee: JMS Co., Ltd.
    Inventors: Koji Suzuki, Seishin Tanaka, Satoshi Hirai, Mari Takabatake
  • Publication number: 20130309659
    Abstract: The present invention discloses a cell culturing formulation and a culturing and quantification method of CD140b+ cells thereof. The cell culturing formulation is applicable for inducing the growth of the CD140b+ cells in peripheral blood. The cell culturing formulation comprises a culturing medium, a serum, a mixed additive and a defined factor. Wherein, concentrations of the culturing medium, the serum, the mixed additive and the defined factor are 59˜98% (v/v), 0.1˜20% (v/v), 1˜10% (v/v) and 10?7˜10% (v/v) respectively.
    Type: Application
    Filed: September 13, 2012
    Publication date: November 21, 2013
    Applicant: CHANG-GUNG UNIVERSITY
    Inventor: CHAO-HUNG WANG
  • Patent number: 8557574
    Abstract: A very safe and useful agent for inhibiting fungal growth and the like are provided by the present invention. Specifically, the present invention provides (1) an agent for inhibiting fungal growth comprising hyaluronic acid or a salt thereof excluding a heavy metal salt as the active ingredient, and a method for inhibiting fungal growth, which comprises at least a step of allowing hyaluronic acid or a salt thereof excluding a heavy metal salt to contact with a fungus, (2) an agent for reinforcing activity of inhibiting fungal growth possessed by a cell, which comprises a DNA encoding a hyaluronic acid synthase as the active ingredient, (3) a method for reinforcing activity of inhibiting fungal growth of a cell, which comprises at least a step of transfecting a DNA encoding a hyaluronic acid synthase into the cell, and (4) a method for inhibiting fungal growth, which comprises at least a step of allowing a cell transfected with a DNA encoding a hyaluronic acid synthase to contact with a fungus.
    Type: Grant
    Filed: February 8, 2007
    Date of Patent: October 15, 2013
    Assignee: Seikagaku Corporation
    Inventors: Tatsuji Nishihara, Sumio Akifusa
  • Patent number: 8486700
    Abstract: The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair.
    Type: Grant
    Filed: April 12, 2011
    Date of Patent: July 16, 2013
    Assignee: Artecel Sciences Inc.
    Inventors: Jeffrey Martin Gimble, Yuan-Di Chang Halvorsen, William O. Wilkison
  • Publication number: 20130059384
    Abstract: Compositions and methods comprising bioenergetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures, are described.
    Type: Application
    Filed: April 13, 2012
    Publication date: March 7, 2013
    Applicants: PRESIDENT AND FELLOWS OF HARVARD COLLEGE, THE GENERAL HOSPITAL CORPORATION
    Inventors: JONATHAN L. TILLY, DAVID A. SINCLAIR
  • Publication number: 20130039991
    Abstract: The application is in the field of transgenic (non-human) organisms, sialic acid chemistry, metabolism and antigenicity. More particularly, the invention is related to a method to produce Neu5Gc-free animals and products therefrom comprising disrupting the CMAH gene and thereby reducing or eliminating Neu5Gc from biological material of non-humans.
    Type: Application
    Filed: June 20, 2012
    Publication date: February 14, 2013
    Inventors: Ajit Varki, Anna Maria Hedlund, Dzung Nguyen
  • Publication number: 20130017604
    Abstract: Methods and compositions for directing adipose-derived stromal cells cultivated in vitro to differentiate into cells of the chondrocyte lineage are disclosed. The invention further provides a variety of chondroinductive agents which can be used singly or in combination with other nutrient components to induce chondrogenesis in adipose-derived stromal cells either in cultivating monolayers or in a biocompatible lattice or matrix in a three-dimensional configuration. Use of the differentiated chondrocytes for the therapeutic treatment of a number of human conditions and diseases including repair of cartilage in vivo is disclosed.
    Type: Application
    Filed: September 30, 2011
    Publication date: January 17, 2013
    Applicant: ARTECEL SCIENCES INC.
    Inventors: Yuan-Di C. Halvorsen, William O. Wilkison, Jeffrey Martin Gimble
  • Patent number: 8338175
    Abstract: The present disclosure describes a tissue system with conjunctival cells, including conjunctival stem cells. The conjunctival tissue system is derived from isolated tissue comprising conjunctival cells, and is suitable for restoring ocular surface impairments, particularly those that result from damaged or diseased conjunctiva. The tissue system is generated using a simple single medium culture scheme, and a support material, such as human amniotic membrane. The conjunctival tissue system generate is suitable for transplantation to treat the ocular surface of an eye of a subject that is damaged or diseased.
    Type: Grant
    Filed: February 23, 2007
    Date of Patent: December 25, 2012
    Assignee: Reliance Life Sciences Pvt. Ltd.
    Inventors: Kashyap Subhadra Devi, Kishore Reddy, Viraf Vasania
  • Patent number: 8337829
    Abstract: The subject invention pertains to tumor cell lines useful for increasing the proliferation potential of any human or animal cell in culture, thereby providing immortalized or continuous cell lines and cultures. The invention also concerns proliferation factors, and compositions containing the factors, which are capable of increasing the proliferation potential of any human or other animal cell in culture. The subject invention further pertains to a method for proliferating cells in culture by contacting cells with the proliferation factors. The proliferated cells can range in plasticity and can include, for example, blast cells, fertilized ova, non-fertilized gametes, embryonic stem cells, adult stem cells, precursor or progenitor cells, and highly specialized cells. Optionally, the cells can be induced to cease proliferation.
    Type: Grant
    Filed: October 6, 2011
    Date of Patent: December 25, 2012
    Assignees: University of South Florida, University of Chile
    Inventors: Thomas B. Freeman, Pablo Caviedes, Raul Caviedes
  • Patent number: 8334138
    Abstract: The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.
    Type: Grant
    Filed: August 15, 2010
    Date of Patent: December 18, 2012
    Assignee: ViaCyte, Inc.
    Inventors: Allan J Robins, Thomas C Schulz
  • Publication number: 20120276632
    Abstract: The present invention provides a cell culture medium supplement comprising plasma-free platelet lysate and medium supplemented with this supplement. The present invention further provides a method for preparing the supplement comprising the steps of (a) preparing platelet rich plasma; (b) removing the plasma; and (c) lysing the platelets.
    Type: Application
    Filed: May 30, 2012
    Publication date: November 1, 2012
    Applicant: MEDICAL UNIVERSITY OF GRAZ
    Inventors: Dirk Strunk, Katharina Schallmoser, Eva Rohde
  • Publication number: 20120231542
    Abstract: Compositions and methods for culturing therapeutic cells are provided herein. According to at least one embodiment, compositions comprising cord blood plasma and lysed platelets and methods for making and using same are provided herein.
    Type: Application
    Filed: March 12, 2012
    Publication date: September 13, 2012
    Applicant: GENERAL BIOTECHNOLOGY, LLC
    Inventors: Erik J. Woods, Sreedhar Thirumala, Dan Zhou, W. Scott Goebel
  • Patent number: 8252590
    Abstract: The present invention relates to mammalian cell culture media which comprise supernatant from some of the fractions of human plasma fractionation according to the Cohn method, more specifically, the supernatant of fractions I and II+III. When said supernatant is added as a culture medium supplement it provides various nutrients and factors for the effective maintenance and/or proliferation of the cultured mammalian cells. In addition, the present invention relates to the preparation process and use of said medium in the culture of mammalian cells.
    Type: Grant
    Filed: July 2, 2010
    Date of Patent: August 28, 2012
    Assignee: Grifols, S.A.
    Inventors: Juan Ignacio Jorquera Nieto, Montserrat Costa Rierola, José María Diez Cervantes
  • Patent number: 8222034
    Abstract: The present invention is of methods of establishing and propagating human embryonic stem cell lines using feeder cells-free, xeno-free culture systems and stem cells which are capable of being maintained in an undifferentiated, pluripotent and proliferative state in culture which is free of xeno contaminants and feeder cells.
    Type: Grant
    Filed: April 11, 2011
    Date of Patent: July 17, 2012
    Assignee: Technion Research & Development Foundation Ltd.
    Inventors: Michal Amit, Joseph Itskovitz-Eldor
  • Publication number: 20120148540
    Abstract: The subject invention pertains to tumor cell lines useful for increasing the proliferation potential of any human or animal cell in culture, thereby providing immortalized or continuous cell lines and cultures. The invention also concerns proliferation factors, and compositions containing the factors, which are capable of increasing the proliferation potential of any human or other animal cell in culture. The subject invention further pertains to a method for proliferating cells in culture by contacting cells with the proliferation factors. The proliferated cells can range in plasticity and can include, for example, blast cells, fertilized ova, non-fertilized gametes, embryonic stem cells, adult stem cells, precursor or progenitor cells, and highly specialized cells. Optionally, the cells can be induced to cease proliferation.
    Type: Application
    Filed: October 6, 2011
    Publication date: June 14, 2012
    Applicants: UNIVERSITY OF CHILE, UNIVERSITY OF SOUTH FLORIDA
    Inventors: THOMAS B. FREEMAN, Pablo Caviedes, Raul Caviedes, Paul R. Sanberg, Don F. Cameron
  • Publication number: 20120142104
    Abstract: A method of preparing a supplement for cell cultivation media, which comprises concentrating porcine blood by centrifugation and obtaining supernatant, adding agonist for activating platelets into the supernatant to obtain activated supernatant, and sterilizing the activated supernatant is presented. A supplement for cell cultivation media, which is made by the above method, a cell cultivation media comprising the supplement, and a use of the cell cultivation media in culturing or treating cells in tissue engineering or regenerative medicine is also presented.
    Type: Application
    Filed: July 1, 2011
    Publication date: June 7, 2012
    Inventors: Yung-Kai Lin, Wei-Hsun You
  • Patent number: 8187881
    Abstract: The invention is directed to methods for the treatment of wounds. Such methods utilize novel compositions, including but not limited to amnion-derived multipotent cells (herein referred to as AMP cells), conditioned media derived therefrom (herein referred to as amnion-derived cellular cytokine suspension or ACCS), cell lysates derived therefrom, cell products derived therefrom, each alone or in combination.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: May 29, 2012
    Assignee: Stemnion, Inc.
    Inventors: Charlotte A. Smith, Catherine J. Trumpower, Vivienne S. Marshall
  • Publication number: 20120115161
    Abstract: Serum-containing and serum-free immunoglobulin inhibitors of steroid hormone responsive cancer cell growth are disclosed, along with their methods of production. Also disclosed are defined cell culture media, assay protocols, and model systems using the inhibitors for demonstrating steroid hormone growth effects of natural and synthetic substances, and other cell culture applications. The disclosed compositions and methods employing the immunoglobulin inhibitors are also useful as reagents in research, and for the diagnosis, treatment and prevention of mucus epithelial cancers.
    Type: Application
    Filed: May 20, 2011
    Publication date: May 10, 2012
    Inventor: David A. Sirbasku
  • Publication number: 20120070407
    Abstract: There is disclosed the use of a composition for promoting neuronal growth of neurons in tissues of the central or peripheral nervous system. There is also disclosed a method for inducing proliferation or differentiation of neuronal cells.
    Type: Application
    Filed: March 29, 2010
    Publication date: March 22, 2012
    Inventors: Michel Maurice Jacques Lazdunski, Catherine Louise Heurteaux, David Picard
  • Publication number: 20120064621
    Abstract: The present invention provided cell culture compositions capable of producing fusion polypeptides that bind vascular endothelial growth factor (VEGF). The cell culture compositions of the invention comprise cells which contain an expression vector comprising a nucleic acid molecule encoding a fusion polypeptide that binds VEGF. The fusion polypeptides may comprise a VEGF receptor component having immunoglobulin-like (Ig) domain 2 of a first VEGF receptor, an Ig domain 3 of a second VEGF receptor, and a multimerizing component.
    Type: Application
    Filed: May 13, 2011
    Publication date: March 15, 2012
    Applicant: Regeneron Pharmaceuticals, Inc.
    Inventors: Nicholas J. Papadopoulos, Samuel Davis, George D. Yancopoulos
  • Patent number: 8129183
    Abstract: In the ex vivo stimulation method of dendritic cells, the stimulation occurs with the lysate of tumor spheres (TS) of the solid tumors.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: March 6, 2012
    Inventors: Gaetano Finocchiaro, Alfredo Martini, Serena Pellegatta
  • Publication number: 20120052136
    Abstract: The present invention relates to isolated haemoglobin from worms belonging to the Nereididae family and its use in cell culture medium, in preservation solutions and as artificial oxygen carrier for transfusion.
    Type: Application
    Filed: May 7, 2010
    Publication date: March 1, 2012
    Applicant: HEMARINA
    Inventors: Morgane Rousselot, Delphine Dutheil, Franck Zal
  • Patent number: 8097457
    Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.
    Type: Grant
    Filed: April 13, 2011
    Date of Patent: January 17, 2012
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Nicholas L. Abbott, Jeffrey C. Mohr, Christopher J. Murphy, Elise Huang, Juan J. DePablo, Nathan A. Lockwood, Sean P. Palecek
  • Publication number: 20110256095
    Abstract: The present application discloses matrix compositions to support the repair of tissue defects such as an injury to tendon tissue, ligament tissue, vascular tissue, dermal tissue, or muscle tissue. A matrix described herein comprises a polyester polymer entangled with a polysaccharide polymer. Also disclosed are methods of preparing a matrix, and methods of using a matrix in the repair of tissue. In certain configurations, a matrix can comprise a polyester cross-linked with a polysaccharide, which can be an oxidized polysaccharide. In some configurations, a matrix can further comprise one or more additional components, such as a growth factor or an anti-infective agent. In some configurations, a matrix can be a viscous fluid or a paste, while in other configurations a matrix can be comprised by a solid such as a plug, a granule or a membrane.
    Type: Application
    Filed: June 23, 2011
    Publication date: October 20, 2011
    Applicant: ISTO TECHNOLOGIES, INC.
    Inventors: Mitchell S. Seyedin, Gary Gage
  • Publication number: 20110201087
    Abstract: The present invention relates to virus growth media that improve the yield of alpha-herpesviruses (e.g., HSV-2) grown in cell cultures. The growth media of the invention include two additives, a disaccharide and a lipid mixture, that can be added to serum-free or serum-enriched growth media to improve the efficiency of virus production. The invention further provides methods of producing alpha-herpesviruses (e.g., HSV-2) in such growth media.
    Type: Application
    Filed: August 11, 2009
    Publication date: August 18, 2011
    Applicant: Sanofi Pasteur Biologics Co.
    Inventors: Simon Delagrave, Rachid Oubelaid, John Hamberger
  • Publication number: 20110189751
    Abstract: Compositions, and uses thereof, which are beneficial for eukaryotic cells in culture, and methods for their use in promoting cell growth, viability and recombinant protein expression. The methods disclosed in the present application are useful, for example, for improving cell viability and in accelerating the rate of cell growth of cells grown in culture. In one aspect, the supplements of the invention are useful for improving or enhancing the yield of the recombinant proteins from the cell cultures.
    Type: Application
    Filed: December 17, 2010
    Publication date: August 4, 2011
    Applicant: Ventria Bioscience
    Inventor: Michael E. Barnett
  • Publication number: 20110183414
    Abstract: This invention provides a cell growth medium comprising (a) a human platelet lysate free of solid matter greater than 0.22 ?m in diameter, wherein the lysate constitutes from 2% to 15% of the total volume of the cell growth medium; (b) a human fresh frozen plasma (FFP) filtrate free of solid matter greater than 0.22 ?m in diameter, wherein the FFP filtrate constitutes from 1% to 10% of the total volume of the cell growth medium; (c) heparin at a concentration of from 0 U/ml to 10 U/ml of the cell growth medium; (d) L-glutamine at a concentration of from 0.5 mM to 10 mM; and (e) a serum-free, low glucose medium suitable for mammalian cell growth, wherein the serum-free, low glucose medium constitutes from 75% to 97% of the total volume of the cell growth medium, and may contain the L-glutamine of part (d); wherein the cell growth medium permits the expansion of human CD34? stem cells and wherein the resulting expanded CD34? stem cells retain the ability to differentiate.
    Type: Application
    Filed: December 22, 2010
    Publication date: July 28, 2011
    Applicant: Apceth GmbH & Co. KG
    Inventors: Elena Aseeva, Christine Günther
  • Publication number: 20110171731
    Abstract: This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided.
    Type: Application
    Filed: September 16, 2009
    Publication date: July 14, 2011
    Inventors: Allan B. Dietz, Michael P. Gustafson, Greg W. Butler
  • Patent number: 7947500
    Abstract: The present invention is directed to liquid crystalline substrates useful in the culture of cells and methods of their use. In certain embodiments, the invention provides methods and devices for imaging changes (e.g., reorganization) of extracellular matrix components by living cells.
    Type: Grant
    Filed: May 11, 2010
    Date of Patent: May 24, 2011
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Nicholas L. Abbott, Jeffrey C. Mohr, Christopher J. Murphy, Elise Huang, Juan J. DePablo, Nathan A. Lockwood, Sean P. Palecek
  • Publication number: 20110076253
    Abstract: The present invention relates to compositions and methods for culturing stem cells, particularly embryonic stem cells. Specifically, the invention relates to a culture medium that supports proliferation of substantially undifferentiated stem cells, while maintaining potency of the cells. An an embodiment, the culture medium is defined and supports proliferation of substantially undifferentiated embryonic stem cells in essentially serum free and feeder cell free conditions. Compositions for making the medium and methods using the culture medium are also provided.
    Type: Application
    Filed: July 28, 2006
    Publication date: March 31, 2011
    Inventors: Michael P. Snyder, Joyce J. Lu
  • Publication number: 20100303770
    Abstract: The present invention relates to novel methods of growing or otherwise producing unpassaged or minimally-passaged dermal cells from a small biopsy specimen for treating skin injuries significantly larger than the biopsy.
    Type: Application
    Filed: December 27, 2007
    Publication date: December 2, 2010
    Inventors: John Maslowski, Myrna F. Thomas, Marie A. Lindner