In Presence Of Phenyl Acetic Acid Or Phenyl Acetamide Or Their Derivatives Patents (Class 435/46)
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Patent number: 6995045Abstract: The thin film transistor has a non-transparent structure besides and insulated with the gate. Hence, the light transmitted from the substrate is blocked and the light current induced in the thin film transistor is negligible. The method uses a mask with a slit pattern to form a non-uniform photoresist. Hence, the mask could be used to pattern two conductor layers for forming source/drain/channel.Type: GrantFiled: March 5, 2003Date of Patent: February 7, 2006Assignee: Chunghwa Picture Tubes, Ltd.Inventors: Hung-Jen Chu, Nei-Jen Hsiao, Hui-Chung Shen, Meng-Chi Liou
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Publication number: 20040009608Abstract: A method of fabricating an array of different chemical moieties. The method may include ejecting drops containing the different moieties or precursors thereof from an orifice in an ejector head onto a substrate surface spaced from the orifice so as to form the array. The substrate surface may have structures adjacent each of multiple feature locations so as to assist in confining drops ejected onto the surface to the feature locations, which structures comprise channels or deposited raised members adhering to the surface and extending above adjacent feature locations. An array of different chemical moieties is also provided which has features at respective locations on a planar substrate surface having structures comprising channels or deposited raised barriers adhering to the surface, and which structures are adjacent each of multiple feature locations.Type: ApplicationFiled: July 10, 2002Publication date: January 15, 2004Inventors: Michael P. Caren, William D. Fisher, Peter G. Webb
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Publication number: 20030190695Abstract: A mutated expandase enzyme having higher activity on penicillin G is provided to produce phenylacetyl-7-aminodeacetoxycephalosporanic acid (7-ADCA), which mutated expandase enzyme has one or more amino acid substitutions selected form M73T, S79E, V275I, L277K, C281Y, G300V, N304K, I305L and 1305M.Type: ApplicationFiled: March 26, 2002Publication date: October 9, 2003Inventors: Yang Yunn-Bor, Wei Chia-Li, Hsu Jyh-Shing, Tsai Ying-Chieh
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Publication number: 20030186354Abstract: A mutated expandase enzyme having higher activity on penicillin G is provided to produce 7-aminodeacetoxycephalosporanic acid (7-ADCA), which mutated expandase enzyme has one or more amino acid substitutions selected form methionine 73, glycine 79, valine 275, leucine 277, cysteine 281, glycine 300, asparagine 304, isoleucine 305, threonine 91, alanine 106, cysteine 155, tyrosine 184, methionine 188 and histidine 244, provided that the amino acid substitution at the residue position of asparagine 304 is not N304L and the amino acid substitution at the residue position of cysteine 155 is C155Y.Type: ApplicationFiled: September 9, 2002Publication date: October 2, 2003Inventors: Yang Yunn-Bor, Wei Chia-Li, Hsu Jyh-Shing, Tsai Ying-Chieh
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Patent number: 6518039Abstract: The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.Type: GrantFiled: May 17, 1999Date of Patent: February 11, 2003Assignee: DSM N.V.Inventors: Maarten Nieboer, Erik De Vroom, Johannis Lugtenburg, Dirk Schipper, Adrianus Wilhelmus Hermanus Vollebregt, Roelof Ary Lans Bovenberg
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Patent number: 6503727Abstract: The invention relates to a process for the preparation of an antibiotic, in particular cefalexin, ampicillin, amoxicillin, cefaclor, cefradin, cefadroxil, cefotaxim and the like, wherein a beta-lactam core is acylated, the antibiotic is recovered from the reaction mixture, the remaining mother liquor is subjected to a hydrolysis reaction in which the antibiotic present in the mother liquor is decomposed into its initial compounds, in particular the beta-lactam core, and the acylation agent is hydrolized. The beta-lactam core can then be recovered virtually quantitatively or recycled to the acylation reaction. This is because it has been found that the solubility of the beta-lactam core is unexpectedly high at relatively high concentrations of acylation agent and antibiotic.Type: GrantFiled: June 5, 1998Date of Patent: January 7, 2003Assignee: Gist-Brocades, B.V.Inventors: Wilhelmus H. J. Boesten, Wilhelmus J. J. Van Den Tweel, Rocus M. Dekkers
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Publication number: 20020127634Abstract: Method and compositions are provided for the determination of telomere length and telomerase activity, as well as the ability to inhibit telomerase activity in the treatment of proliferative diseases. Particularly, primers are elongated under conditions which minimize interference from other genomic sequences, so as to obtain accurate determinations of telomeric length or telomerase activity. In addition, compositions are provided for intracellular inhibition of telomerase activity and means are shown for slowing the loss of telomeric repeats in aging cells.Type: ApplicationFiled: June 5, 1995Publication date: September 12, 2002Inventors: MICHAEL D. WEST, JERRY SHAY, WOODRING WRIGHT, ELIZABETH H. BLACKBURN
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Patent number: 6323321Abstract: The present invention relates to a monoclonal antibody binding an antigen on the megakaryocytic cell line UT-7. The invention further relates to hybridoma cells producing such an antibody.Type: GrantFiled: June 30, 1999Date of Patent: November 27, 2001Assignee: Eberhard-Karls-Universitat Tubingen UniversitatsklinikumInventor: Hans-Jörg Bühring
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Patent number: 6156534Abstract: Disclosed is a process for the synthesis of .beta.-lactam antibacterials using soluble side chain esters in the presence of enzyme acylase. Also disclosed are novel esters useful as reactants in said process.Type: GrantFiled: October 22, 1998Date of Patent: December 5, 2000Assignee: Bristol-Myers Squibb CompanyInventors: John J. Usher, Guna Romancik
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Patent number: 6110699Abstract: Alternative process for obtaining 6-aminopenicillanic acid. The process comprises replacing the stages of extraction with organic solvents and isolation and separation of the intermediate penicillin salt as a solid by a process of ultrafiltration of the culture broth in at least 2 successive stages. The first stage has a cut-off for molecular weights of 20,000 Dalton and the second, 2000 Dalton. Subsequent to the enzyme conversion stage the products from that stage are subjected to a series of anionic exchange chromatography steps.Type: GrantFiled: February 25, 1998Date of Patent: August 29, 2000Assignee: Antibioticos, S.A.Inventors: Manuel Oliver Ruiz, Nieves Fraile Yecora, Emiliano Gonzalez De Prado, Alejandro Vitaller Alba, Francisco Salto Maldonado
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Patent number: 6020151Abstract: A process is taught for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum transformant strain expressing expandase.Type: GrantFiled: February 18, 1998Date of Patent: February 1, 2000Assignee: DSM N.V.Inventors: Roelof Ary Lans Bovenberg, Bertus Pieter Koekman, Dirk Schipper, Adrianus Wilhelmus Hermanus Vollebregt
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Patent number: 5795733Abstract: An overall efficient process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via 3-(carboxyethylthio)propionyl-7-ADCA, using a Penicillium chrysogenum transformant strain expressing expandase in conjunction with acyltransferase, is provided.Type: GrantFiled: May 13, 1996Date of Patent: August 18, 1998Assignee: Gist-Brocades, B.V.Inventors: Roelof Ary Lans Bovenberg, Bertus Pieter Koekman, Andreas Hoekema, Jan Metske Van Der Laan, Jan Verweij, Erik De Vroom
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Patent number: 5753458Abstract: A method for providing a semisynthetic .beta.-lactam antibiotic by enzyme catalyzed acylation of the parent .beta.-lactam with an activated derivative of the side chain acid wherein a modulator, which consists of one or more compounds different from the reactants and the reaction product and which suppresses the hydrolysis of the activated derivative of the side chain acid and the desired product more than it suppresses the synthesis of the desired product, is added to the reaction mixture, at the beginning of the reaction process, in a concentration from about 0.2 to 100.times.10.sup.3 .mu.m.Type: GrantFiled: February 7, 1996Date of Patent: May 19, 1998Assignee: Gist-Brocades B.V.Inventors: Kim Clausen, Annette Nielsen, Niels Petersen, Alexander Nikolov
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Patent number: 5731165Abstract: An overall process for the preparation and recovery of 7-aminodesacetoxycephalosporanic acid (7-ADCA) via enzymatic ring expansion activity on penicillin G, using a Penicillium chrysogenum transformant strain expressing expandase.Type: GrantFiled: June 2, 1995Date of Patent: March 24, 1998Assignee: Gist-brocades, B.V.Inventors: Roelof Ary Lans Bovenberg, Bertus Pieter Koekman, Dirk Schipper, Adrianus Wilhelmus Vollebregt
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Patent number: 5656422Abstract: Methods and compositions are provided for the detection of 2,4-dichlorophenoxyacetic acid (2,4-D) and other phenoxy ether compounds. The phenoxy ether bond of 2,4-D is enzymatically cleaved by 2,4-D .alpha.-ketoglutarate dioxygenase to form 2,4-dichlorophenol, which is assayed by the 4-aminoantipyrine method. The enzyme is supplied in a dried form, preferably immobilized on a solid support, and is stable at room temperature for several months even in a highly impure state, e.g., crude cell extracts or dried cells.Type: GrantFiled: October 5, 1994Date of Patent: August 12, 1997Assignee: Idaho Research Foundation, Inc.Inventors: Ronald L. Crawford, Yongxiang Gu, Roger A. Korus, David B. Knaebel
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Patent number: 5525483Abstract: .beta.-Lactam derivatives are synthesized by an enzymatic reaction of the parent amino .beta.-lactam with the corresponding acylating agent, the concentration of the acylating agent plus the concentration of .beta.-lactam derivative in the reaction mixture being above about 400 mM.Type: GrantFiled: January 18, 1994Date of Patent: June 11, 1996Assignee: Gist Brocades B.V.Inventors: Svend G. Kaasgaard, Ulla Veitland
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Patent number: 5268271Abstract: Synthesis of semi-synthetic monobactamic or .beta.-Lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a thermodynamically controlled strategy in monophase water/cosolvent organic apolar systems, wherein the concentration of the cosolvent varies between 30% and 90%, the temperature between -10.degree. C. and 50.degree. C., the pH between 4.5 and 8.5, with concentrations of the antibiotic nucleus between 0.5 an 875 mM and acyl donor between 0.2 mM and 1M, with a relationship antibiotic ring/activated or free acyl donor, using a buffer between 0 and 1M. Application to the pharmaceutical industry.Type: GrantFiled: August 2, 1991Date of Patent: December 7, 1993Assignee: Cosejo Superior de Invetigaciones CertificasInventors: Jose M. Guisan Seijas, Roberto Fernandez Lafuente, Gregorio Alvaro Campos, Rosa M. Blanco Martin, Cristina Molina Rosell
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Patent number: 5192671Abstract: Avermectin compounds are glycosylated the 4' and 4"-positions by adding the avermectin compounds to the fermentation medium of Saccharapolyspora erythrea. The outer oleandrose sugar group of the avermectin compound is glycosylated with a glycosyl moiety, specifically a glucose group. In addition, other changes are effected in the avermectin moiety such as selective hydroxylation, epimerization at the 2-carbon and migration of the .DELTA. 3-double bond to a .DELTA. 2-position.Type: GrantFiled: June 26, 1991Date of Patent: March 9, 1993Assignee: Merck & Co., Inc.Inventors: Byron H. Arison, Marvin D. Schulman, Patrick J. Doherty
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Patent number: 5079146Abstract: The present invention relates to a new method of protection of the carboxy group in the chemistry of the compounds of .beta.-lactam type. According to such a method, the carboxy group is protected by being transformed into its corresponding phenyl-acetoxy-methyl ester, which is then removed by an enzymatic route by means of penicillinacylase.In particular, if the .beta.-lactam compound also bears a phenyl-acetamidic substitutent, this latter is simultaneously hydrolysed during the same step of removal of the carboxy function protecting group.Type: GrantFiled: April 4, 1989Date of Patent: January 7, 1992Assignee: Sclavo S.p.A.Inventors: Claudio Fuganti, Eva Baldaro, Daniela Faiardi, Ameriga Lazzarini
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Patent number: 4208481Abstract: Benzylpenicillin is prepared by culturing a penicillin-producing microorganism in a medium containing suitable sources of nitrogen, carbon and energy, and inorganic salts. One or more phenylalkanes of the formula ##STR1## wherein n is an integer from 6 to 13 are included within the medium as the sidechain precursor.Type: GrantFiled: November 16, 1978Date of Patent: June 17, 1980Assignee: E. R. Squibb & Sons, Inc.Inventors: Laszlo J. Szarka, Robert W. Eltz
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Patent number: 4164445Abstract: Ethanol is employed as the major source of carbon and energy in the fermentative biosynthesis of penicillins.Type: GrantFiled: March 27, 1975Date of Patent: August 14, 1979Assignee: E. R. Squibb & Sons, Inc.Inventors: Brian T. Sheehan, Judith Baymiller, Robert W. Eltz