Abstract: The present invention relates to a safflower plant or part thereof involving the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7, wherein the plant produces and accumulates chymosin in seed under agricultural conditions. A plant seed involving the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7. A consumer product produced from the seed, defined as chymosin, and additionally as ground grain, flour, flakes, oil, biodiesel, biogas, or another biomaterial. Also, the present invention include a recombinant DNA molecule involved in the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7. A DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of the DNA molecule involved in the molecular stacking of safflower events IND-1ØØØ3-4×IND-1ØØ15-7, or its complement which is useful in a DNA amplification method to produce a diagnostic amplicon for the event IND-1ØØØ3-4 and IND-1ØØ15-7, or each of them separately IND-1ØØØ3-4 and IND-1ØØ15-7.

Abstract: A targeted DNA demethylation method (Mini-CRISD) and a fusion protein used in the method are provided. The Mini-CRISD method can targets delivery of demethylation activity through engineered miniature dCjCas9 to deliver engineered miniature ROS1 demethylation effector to specific DNA sequences and/or specific genomic locations (such as CpG islands) to achieve targeted demethylation of specific DNA.

Abstract: According to the invention, there is provided seed and plants of the hybrid corn variety designated CH010980. The invention thus relates to the plants, seeds and tissue cultures of the variety CH010980, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010980 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH010980.

Abstract: The purpose of the present invention is to provide, for translation in a cell-free translation system, a novel translation system capable of synthesizing a peptide having therein consecutive non-proteinogenic amino acids. The present invention provides a tRNA containing the base sequence represented by SEQ ID NO: 1 and encoding a non-proteinogenic amino acid.

Abstract: Provided is a modified rice endosperm-specific promoter, which has a sequence as shown in any one of SEQ ID. NOs. 1 to 5. The EnhGt13? promoter as shown in SEQ ID NO. 2 drives GUS activity in vitro and mediates the expression of foreign proteins in rice endosperm cells, which is significantly higher than that of the unmodified Gt13? promoter. The promoter can be applied to the fields of improving seed quality, molecular pharming, etc.

Abstract: Methods for the detection of components from biological samples are provided. In certain aspects, the methods may be used to detect and/or quantify specific components in a biological sample, such as tumor cells (e.g., circulating tumor cells). Systems and devices for practicing the subject methods are also provided.

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV502253. The invention thus relates to the plants, seeds and tissue cultures of the variety CV502253, and to methods for producing a corn plant produced by crossing a corn plant of variety CV502253 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV502253 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV502253.

Abstract: Disclosed herein are compositions and methods for effecting alterations at a defined location in the genome of a plant cell or plant protoplast. Further disclosed are methods using an RNA-guided nuclease to alter a target nucleotide sequence in a cell or protoplast of a plant; embodiments of such methods include treatments with chemical or physical reagents or treatment of the cell or protoplast with a specific thermal regime, such as a heat treatment.

Abstract: The present invention includes cauliflowers with enhanced whiteness and methods for obtaining such cauliflowers. The present invention also provides reagents that can be used in methods for obtaining such cauliflowers.

Abstract: The present disclosure involves the production of Waxy maize. Compositions and methods are provided for knocking out expression of the Waxy (Wx1) gene in maize by making double strand breaks at one or more target sites in an endogenous WX1 encoding sequence. Some methods employ one or more guide polynucleotides and a Cas endonuclease, wherein Cas endonuclease is guided by the one or more guide polynucleotides to recognize and introduce double strand breaks at specific target sites in and around the Wx1 gene. Also provided are compositions and methods for the production of Waxy maize plant cells, plant explants, seeds and grain.

Abstract: A mutant granule-bound starch synthase 1 (GBSS1) polypeptide and a nucleic acid, and use thereof are provided. Compared to an amino acid sequence of a parent GBSS1, the mutant GBSS1 polypeptide has a mutation at an amino acid corresponding to one or more of amino acid 237, amino acid 168, and amino acid 411 of an amino acid sequence shown in SEQ ID NO: 1. An amylose content (AC) in a plant changes after the plant undergoes GBSS1 mutation, which has very promising application prospects in the improvement of edible quality of rice.

Abstract: The disclosure relates to gene expression modulation elements from plants and their use in modulating the expression of one or more heterologous nucleic acid fragments in plants. The disclosure further discloses compositions, polynucleotide constructs, transformed host cells, plants and seeds containing the expression modulating elements, and methods for preparing and using the same.

Abstract: Presented are RNA nucleotide sequences referred to as EXO-Codes, and longer RNA polynucleotides that contain the EXO-Codes. EXO-Codes provide RNA with the ability to a) be selectively sorted to extracellular vesicles such as exosomes, and b) deliver a variety of cargo types to program or reprogram the extracellular vesicles, and cells that receive the exosomes. Also presented are methods of making the EXO-Codes, modifying cells using the EXO-Codes, expression vectors encoding the EXO-Codes, and exosomes and other secreted vesicles that include RNA polynucleotides that contain the EXO-Codes.

Abstract: Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

Abstract: A bio-lubricant composition includes a first component that includes a first triglyceride, which is part of a cooking oil; a second component that includes a first epoxidized triglyceride; a third component that includes a hydroxylated triglyceride; a fourth component that includes a first fatty acid ester moiety; a fifth component that includes a first epoxidized fatty acid ester; and a sixth component that includes a hydroxylated fatty acid ester. A mixture of the first to sixth components at room temperature have a viscosity between 40 and 200 centipoise, and the composition is substantially free of free fatty acids.

Abstract: Methods are provided to mutate, in a targeted manner, the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants, in particular Brassica plants that yield seeds producing oils having a reduced total saturated fatty acid content, and method for making such plants.

Abstract: This invention relates to methods of controlling gene expression or gene suppression in eukaryotic cells. One aspect of this invention includes modifying the degree of silencing of a target gene by use of a modified suppression element. Another aspect includes providing a eukaryotic cell having a desired phenotype resulting from transcription in the eukaryotic cell of a modified suppression element. Also provided are transgenic eukaryotic cells, transgenic plant cells, plants, and seeds containing modified suppression elements, and useful derivatives of such transgenic plant cells, plants, or seeds, such as food or feed products.

Abstract: This invention relates to crop plants of which the fruit dehiscence properties are modulated. More specifically the invention relates to improved methods and means for reducing seed shattering, or delaying seed shattering until after harvest, while maintaining at the same time an agronomically relevant treshability of the pods, and for increasing yield.

Abstract: The present invention is in the field of plant molecular biology and provides methods for production of high expressing constitutive promoters and the production of plants with enhanced constitutive expression of nucleic acids wherein nucleic acid expression enhancing nucleic acids (NEENAs) are functionally linked to the promoters and/or introduced into plants.

Abstract: A novel maize variety designated PH4CYJ and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4CYJ with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4CYJ through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4CYJ or a locus conversion of PH4CYJ with another maize variety.

Abstract: This application provides improved methods of editing the genome of a target cell. Cas9 molecules can be used to create a break in a genomic region of interest. To increase the likelihood that the break is repaired by homology-directed repair (HDR), the cell can be contacted with an HDR-enhancer. The cell may be, e.g., a human cell, a non-human animal cell, a bacterial cell, or a plant cell.

Abstract: A novel maize variety designated 1PFKS71 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PFKS71 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PFKS71 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PFKS71 or a locus conversion of 1PFKS71 with another maize variety.

Abstract: The present invention relates to processes for extracting lipid from vegetative plant parts such as leaves, stems, roots and tubers, and for producing industrial products such as hydrocarbon products from the lipids. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.

Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Abstract: The subject matter disclosed herein is generally directed to methods and compositions for tagging cells of interest, tracking evolution of the tagged cells, and recovering the original tagged cells for further study. Specifically, cells are tagged with a DNA construct encoding a barcode sequence comprising a guide sequence. Barcoded cells can then be recovered using a reporter construct having CRISPR target sequences specific for the cell having a barcode of interest.

Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

Abstract: Disclosed herein are compostions and methods for improving or enhancing pathogen resistance in legume plants. Compositions comprising polypeptides encoded by legume-derived nucleotide-binding site-leucine-rich repeat (NB-LRR) genes are useful in improving resistance in legumes to Asian soybean rust. Methods of using NB-LRR genes can be used to make a transgenic resistant legume plant.

Abstract: The present invention relates to methods and hybrids for the targeted modification of a nucleic acid-target region in a plant target structure using CRISPR/Cas systems. The invention specifically relates to methods and hybrids for directly obtaining a plant or plant material which comprises an editing of a nucleic acid introduced in a targeted manner into a meristematic cell. The hybrids can be introduced in a transient and/or stable manner. The invention also relates to novel plant-optimized introduction strategies. The invention further relates to a method for carrying out an in vitro screening assay in order to first check the suitable gRNA candidates in vitro with respect to their efficiency.

Abstract: A novel maize variety designated X13P503 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13P503 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13P503 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13P503, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13P503 are provided. Methods for producing maize varieties derived from maize variety X13P503 and methods of using maize variety X13P503 are disclosed.

Abstract: An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.

Abstract: A novel maize variety designated PH47P0 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH47P0 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH47P0 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH47P0 or a locus conversion of PH47P0 with another maize variety.

Abstract: Provided are a cosmetic composition for inhibiting sebum hypersecretion and a method of inhibiting sebum hypersecretion using the same. According to the present invention, there may be provided the cosmetic composition of inhibiting and adjusting sebum secretion which is harmless to the human body with excellent skin safety and decreases activity of peroxisome proliferator activated receptor gamma (PPAR-?) to fundamentally decrease sebum overproduction, thereby providing the skin with an effective effect, and a method of using the same.

Abstract: Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

Abstract: A novel maize variety designated PH4DK3 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4DK3 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4DK3 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4DK3 or a locus conversion of PH4DK3 with another maize variety.

Abstract: Strategies, reagents, methods, and systems for modulating the mutation rate in cells are provided herein. The strategies, reagents, methods, and systems are broadly applicable for the modulation of mutation rates in cells where high mutation rates and/or control over a broad range of mutation rates is desired, for example, in the context of diversifying a nucleic acid sequence or a plurality of such sequences within a population of cells, for the generation of diversified nucleic acid libraries, and for directed evolution of nucleic acids and encoded products.

Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.

Abstract: Coffee beans having an increased flavor component, a method for increasing a flavor component in coffee beans, and a method for evaluating coffee beans are provided. A fatty acid methyl ester content of coffee beans is increased.

Abstract: Provided are a novel amide compound and a use thereof. According to the present invention, there may be provided a use of a novel amide compound which is harmless to the human body with excellent skin stability and decreases activity of peroxisome proliferator activated receptor gamma (PPAR-?) to fundamentally inhibit sebum overproduction, thereby providing the skin with an effective effect, and a cosmetic composition including the same.

Abstract: Inbred corn line, designated KW9Q0906, is disclosed. The invention relates to the seeds of inbred corn line KW9Q0906, to the plants and plant parts of inbred corn line KW9Q0906 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line KW9Q0906 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line KW9Q0906 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line KW9Q0906.

Abstract: Provided herein are microorganisms that include one or more heterologous nucleic acid selected from the group of a sequence encoding a 7-methylxanthosine synthase, a sequence encoding a theobromine synthase; and a sequence encoding a caffeine synthase, where the microorganism is capable of producing one or more purine alkaloid in a culture medium, when the microorganism is cultured under conditions sufficient to produce the one or more purine alkaloid. Also provided compositions and kits that include at least one of these microorganisms, and methods of producing one or more purine alkaloid that include culturing one of these microorganisms under conditions sufficient to produce the one or more purine alkaloid.

Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.

Abstract: Variants of chymosin with improved milk-clotting properties.

Abstract: A novel maize variety designated X13N230 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13N230 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13N230 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13N230, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13N230 are provided. Methods for producing maize varieties derived from maize variety X13N230 and methods of using maize variety X13N230 are disclosed.

Abstract: Materials and methods for genome engineering through transient expression of a targeted nuclease are described herein. For example, the methods described herein can include introducing into a cell a messenger RNA (mRNA) that encodes a nuclease targeted to a selected sequence within the cell, where the stability of the mRNA is modified by the addition of untranslated regions (UTRs).

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV773381. The invention thus relates to the plants, seeds and tissue cultures of the variety CV773381, and to methods for producing a corn plant produced by crossing a corn plant of variety CV773381 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV773381 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV773381.

Abstract: The present invention relates to laccase variants comprising a substitution at one or more positions corresponding to positions 9, 21, 37, 79, 102, 170, 175, 178, 179, 200, 262, 275, 276, 289, 292, 333, 357, 360, 393, 397, 418, 485, 506, and 518 of the full-length polypeptide of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

Abstract: The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5? and 3? ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL pesticidal proteins are also provided.