Abstract: This application provides improved methods of editing the genome of a target cell. Cas9 molecules can be used to create a break in a genomic region of interest. To increase the likelihood that the break is repaired by homology-directed repair (HDR), the cell can be contacted with an HDR-enhancer. The cell may be, e.g., a human cell, a non-human animal cell, a bacterial cell, or a plant cell.

Abstract: A novel maize variety designated 1PFKS71 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety 1PFKS71 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into 1PFKS71 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety 1PFKS71 or a locus conversion of 1PFKS71 with another maize variety.

Abstract: The present invention relates to processes for extracting lipid from vegetative plant parts such as leaves, stems, roots and tubers, and for producing industrial products such as hydrocarbon products from the lipids. Preferred industrial products include alkyl esters which may be blended with petroleum based fuels.

Abstract: The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Abstract: The subject matter disclosed herein is generally directed to methods and compositions for tagging cells of interest, tracking evolution of the tagged cells, and recovering the original tagged cells for further study. Specifically, cells are tagged with a DNA construct encoding a barcode sequence comprising a guide sequence. Barcoded cells can then be recovered using a reporter construct having CRISPR target sequences specific for the cell having a barcode of interest.

Abstract: Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

Abstract: Disclosed herein are compostions and methods for improving or enhancing pathogen resistance in legume plants. Compositions comprising polypeptides encoded by legume-derived nucleotide-binding site-leucine-rich repeat (NB-LRR) genes are useful in improving resistance in legumes to Asian soybean rust. Methods of using NB-LRR genes can be used to make a transgenic resistant legume plant.

Abstract: The present invention relates to methods and hybrids for the targeted modification of a nucleic acid-target region in a plant target structure using CRISPR/Cas systems. The invention specifically relates to methods and hybrids for directly obtaining a plant or plant material which comprises an editing of a nucleic acid introduced in a targeted manner into a meristematic cell. The hybrids can be introduced in a transient and/or stable manner. The invention also relates to novel plant-optimized introduction strategies. The invention further relates to a method for carrying out an in vitro screening assay in order to first check the suitable gRNA candidates in vitro with respect to their efficiency.

Abstract: A novel maize variety designated X13P503 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13P503 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13P503 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13P503, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13P503 are provided. Methods for producing maize varieties derived from maize variety X13P503 and methods of using maize variety X13P503 are disclosed.

Abstract: An insect-resistant herbicide-tolerant corn transformation event, and a related creation method, a detection method, and application thereof are provided herein. Using the corn inbred line Xiang 249 as a receptor, by means of agrobacterium-mediated genetic transformation, obtaining a corn plant with an exogenous gene insert inserted at a specific genomic locus, the exogenous gene insert comprising the following three genes: an insect-resistant gene, a glufosinate resistant gene, and a glyphosate resistant gene. In the obtained transformation event, the inserted exogenous genes are positioned at a non-functional locus of the corn genome, and do not affect the expression of the other genes of the receptor plant, such that the transgenic corn plant maintains good agronomic traits whilst acquiring insect resistance and herbicide tolerance.

Abstract: A novel maize variety designated PH47P0 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH47P0 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH47P0 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH47P0 or a locus conversion of PH47P0 with another maize variety.

Abstract: Provided are a cosmetic composition for inhibiting sebum hypersecretion and a method of inhibiting sebum hypersecretion using the same. According to the present invention, there may be provided the cosmetic composition of inhibiting and adjusting sebum secretion which is harmless to the human body with excellent skin safety and decreases activity of peroxisome proliferator activated receptor gamma (PPAR-?) to fundamentally decrease sebum overproduction, thereby providing the skin with an effective effect, and a method of using the same.

Abstract: Disclosed are methods of obtaining clonal seeds, methods of plant cloning, methods of screening for maternal plants that produce clonal seeds asexually and methods of increasing yield of clonal seeds. Also disclosed are constructs comprising a nucleic acid that may silence the activity of a RNA-dependent DNA methylation pathway gene. Further disclosed are maternal plants comprising a construct wherein the construct comprises an exogenous nucleic acid sequence, wherein the construct renders the maternal plant defective for RNA-dependent DNA methylation.

Abstract: A novel maize variety designated PH4DK3 and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH4DK3 with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH4DK3 through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH4DK3 or a locus conversion of PH4DK3 with another maize variety.

Abstract: Strategies, reagents, methods, and systems for modulating the mutation rate in cells are provided herein. The strategies, reagents, methods, and systems are broadly applicable for the modulation of mutation rates in cells where high mutation rates and/or control over a broad range of mutation rates is desired, for example, in the context of diversifying a nucleic acid sequence or a plurality of such sequences within a population of cells, for the generation of diversified nucleic acid libraries, and for directed evolution of nucleic acids and encoded products.

Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.

Abstract: Coffee beans having an increased flavor component, a method for increasing a flavor component in coffee beans, and a method for evaluating coffee beans are provided. A fatty acid methyl ester content of coffee beans is increased.

Abstract: Provided are a novel amide compound and a use thereof. According to the present invention, there may be provided a use of a novel amide compound which is harmless to the human body with excellent skin stability and decreases activity of peroxisome proliferator activated receptor gamma (PPAR-?) to fundamentally inhibit sebum overproduction, thereby providing the skin with an effective effect, and a cosmetic composition including the same.

Abstract: Inbred corn line, designated KW9Q0906, is disclosed. The invention relates to the seeds of inbred corn line KW9Q0906, to the plants and plant parts of inbred corn line KW9Q0906 and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line KW9Q0906 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line KW9Q0906 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line KW9Q0906.

Abstract: Provided herein are microorganisms that include one or more heterologous nucleic acid selected from the group of a sequence encoding a 7-methylxanthosine synthase, a sequence encoding a theobromine synthase; and a sequence encoding a caffeine synthase, where the microorganism is capable of producing one or more purine alkaloid in a culture medium, when the microorganism is cultured under conditions sufficient to produce the one or more purine alkaloid. Also provided compositions and kits that include at least one of these microorganisms, and methods of producing one or more purine alkaloid that include culturing one of these microorganisms under conditions sufficient to produce the one or more purine alkaloid.

Abstract: The current disclosure relates to the field of plants, in particular to the fields of plant breeding and plant genetics. More particular, the disclosure concerns inventive methodology that may be useful in improving plant properties. In particular the invention may be useful in removing linkage drag. Also provided are plant and plant parts obtained with the method disclosed herein.

Abstract: Variants of chymosin with improved milk-clotting properties.

Abstract: A novel maize variety designated X13N230 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X13N230 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X13N230 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X13N230, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X13N230 are provided. Methods for producing maize varieties derived from maize variety X13N230 and methods of using maize variety X13N230 are disclosed.

Abstract: Materials and methods for genome engineering through transient expression of a targeted nuclease are described herein. For example, the methods described herein can include introducing into a cell a messenger RNA (mRNA) that encodes a nuclease targeted to a selected sequence within the cell, where the stability of the mRNA is modified by the addition of untranslated regions (UTRs).

Abstract: According to the invention, there is provided seed and plants of the corn variety designated CV773381. The invention thus relates to the plants, seeds and tissue cultures of the variety CV773381, and to methods for producing a corn plant produced by crossing a corn plant of variety CV773381 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV773381 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV773381.

Abstract: The present invention relates to laccase variants comprising a substitution at one or more positions corresponding to positions 9, 21, 37, 79, 102, 170, 175, 178, 179, 200, 262, 275, 276, 289, 292, 333, 357, 360, 393, 397, 418, 485, 506, and 518 of the full-length polypeptide of SEQ ID NO: 2. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

Abstract: The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Abstract: An Engineered Transgene Integration Platform (ETIP) is described that can be inserted randomly or at targeted locations in plant genomes to facilitate rapid selection and detection of a GOI that is perfectly targeted (both the 5? and 3? ends) at the ETIP genomic location. One element in the subject disclosure is the introduction of specific double stranded breaks within the ETIP. In some embodiments, an ETIP is described using zinc finger nuclease binding sites, but may utilize other targeting technologies such as meganucleases, CRISPRs, TALs, or leucine zippers. Also described are compositions of, and methods for producing, transgenic plants wherein the donor or payload DNA expresses one or more products of an exogenous nucleic acid sequence (e.g. protein or RNA) that has been stably-integrated into an ETIP in a plant cell. In embodiments, the ETIP facilitates testing of gene candidates and plant expression vectors from ideation through Development phases.

Abstract: Pesticidal proteins exhibiting toxic activity against Lepidopteran pest species are disclosed, and include, but are not limited to, TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL. DNA constructs are provided which contain a recombinant nucleic acid sequence encoding one or more of the disclosed pesticidal proteins. Transgenic plants, plant cells, seed, and plant parts resistant to Lepidopteran infestation are provided which contain recombinant nucleic acid sequences encoding the pesticidal proteins of the present invention. Methods for detecting the presence of the recombinant nucleic acid sequences or the proteins of the present invention in a biological sample, and methods of controlling Lepidopteran species pests using any of the TIC4472, TIC4472PL, TIC1425, TIC2613, and TIC2613PL pesticidal proteins are also provided.

Abstract: The present invention relates to the production of a non-transgenic plant resistant or tolerant to a herbicide of the phosphonomethylglycine family, e.g., glyphosate. The present invention also relates to the use of a recombinagenic oligonucleobase to make a desired mutation in the chromosomal or episomal sequences of a plant in the gene encoding for 5-enol pyruvylshikimate-3-phosphate synthase (EPSPS). The mutated protein, which substantially maintains the catalytic activity of the wild-type protein, allows for increased resistance or tolerance of the plant to a herbicide of the phosphonomethylglycine family, and allows for the substantially normal growth or development of the plant, its organs, tissues or cells as compared to the wild-type plant irrespective of the presence or absence of the herbicide. Additionally the present invention relates to mutant E. coli cells that contain mutated EPSPS genes.

Abstract: A novel maize variety designated PH473G and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH473G with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH473G through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH473G or a locus conversion of PH473G with another maize variety.

Abstract: The invention relates to the alfalfa variety designated R410A109. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R410A109. Also provided by the invention are tissue cultures of the alfalfa variety R410A109 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R410A109 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated R410A136. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R410A136. Also provided by the invention are tissue cultures of the alfalfa variety R410A136 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R410A136 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated R410A131. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R410A131. Also provided by the invention are tissue cultures of the alfalfa variety R410A131 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R410A131 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated R410M327. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R410M327. Also provided by the invention are tissue cultures of the alfalfa variety R410M327 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R410M327 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 49H344. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 49H344. Also provided by the invention are tissue cultures of the alfalfa variety 49H344 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 49H344 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 410A179. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 410A179. Also provided by the invention are tissue cultures of the alfalfa variety 410A179 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 410A179 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated 410A177. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety 410A177. Also provided by the invention are tissue cultures of the alfalfa variety 410A177 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety 410A177 with itself or another alfalfa variety and plants produced by such methods.

Abstract: There is disclosed a mutant, non-naturally occurring or transgenic plant cell comprising: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence encoding a threonine synthase and having at least 90% sequence identity to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 or at least 87% sequence identity to SEQ ID NO:4, or SEQ ID NO:5; (ii) a polypeptide encoded by any one of said polynucleotides set forth in (i); or (iii) a polypeptide having at least 95% sequence identity to SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8; or (iv) a construct, vector or expression vector comprising the polynucleotide as set forth in (i).

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

Abstract: The invention relates to the alfalfa variety designated R411A107. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety R411A107. Also provided by the invention are tissue cultures of the alfalfa variety R411A107 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety R411A107 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The present invention provides novel compositions for use to enhance crop performance. Specifically, the present invention provides for delayed senescence and/or improved yield enhancement in various crops. The present invention also provides for combinations of compositions and methods that provide for delayed senescence and/or improved yield.

Abstract: The invention relates to the alfalfa variety designated RRL43M104. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL43M104. Also provided by the invention are tissue cultures of the alfalfa variety RRL43M104 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL43M104 with itself or another alfalfa variety and plants produced by such methods.

Abstract: A novel soybean variety, designated 5PKYK65 is provided. Also provided are the seeds of soybean variety 5PKYK65, cells from soybean variety 5PKYK65, plants of soybean 5PKYK65, and plant parts of soybean variety 5PKYK65. Methods provided include producing a soybean plant by crossing soybean variety 5PKYK65 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety 5PKYK65, methods for producing other soybean varieties or plant parts derived from soybean variety 5PKYK65, and methods of characterizing soybean variety 5PKYK65. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety 5PKYK65 are further provided.

Abstract: The invention relates to the alfalfa variety designated RRL43M113. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL43M113. Also provided by the invention are tissue cultures of the alfalfa variety RRL43M113 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL43M113 with itself or another alfalfa variety and plants produced by such methods.

Abstract: A novel maize variety designated PH2DPF and seed, plants and plant parts thereof are provided. Methods for producing a maize plant comprise crossing maize variety PH2DPF with another maize plant are provided. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH2DPF through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby are provided. Hybrid maize seed, plants or plant parts are produced by crossing the variety PH2DPF or a locus conversion of PH2DPF with another maize variety.

Abstract: The invention relates to the alfalfa variety designated RRL43A108. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL43A108. Also provided by the invention are tissue cultures of the alfalfa variety RRL43A108 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL43A108 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated RRL43M119. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL43M119. Also provided by the invention are tissue cultures of the alfalfa variety RRL43M119 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL43M119 with itself or another alfalfa variety and plants produced by such methods.

Abstract: The invention relates to the alfalfa variety designated RRL43M114. Provided by the invention are the seeds, plants and derivatives of the alfalfa variety RRL43M114. Also provided by the invention are tissue cultures of the alfalfa variety RRL43M114 and the plants regenerated therefrom. Still further provided by the invention are methods for producing alfalfa plants by crossing the alfalfa variety RRL43M114 with itself or another alfalfa variety and plants produced by such methods.