Introduction Via Agrobacterium Patents (Class 435/469)
  • Patent number: 11473094
    Abstract: The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.
    Type: Grant
    Filed: May 11, 2021
    Date of Patent: October 18, 2022
    Assignee: MONSANTO TECHNOLOGY, LLC
    Inventors: Whitney R. Adams, Jr., Brian J. Martinell, Jyoti R. Rout, Edward J. Williams
  • Patent number: 11091767
    Abstract: The present invention provides methods for improving competency of plant cells, such as corn plant immature embryo for bacterial-mediated transformation comprising contacting the plant cells, such as corn plant immature embryo with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells, such as corn plant immature embryo for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells, such as corn plant immature embryo in various storage conditions, thus improving their competency for transformation and tissue culture.
    Type: Grant
    Filed: July 18, 2019
    Date of Patent: August 17, 2021
    Assignee: Monsanto Technology LLC
    Inventors: Whitney R. Adams, Jr., Brian J. Martinell, Jyoti R. Rout, Edward J. Williams
  • Patent number: 10947556
    Abstract: Methods and compositions are provided which allow for genetic modification of host cells including, plants and plant cells. The various methods and composition employ a recombinant DNA construct comprising SEQ ID NO: 1 and/or 2 or active variants and fragments thereof. Such polynucleotides find use in facilitating integration of polynucleotides of interest into the DNA of a host cell, including a plant or plant cell. Vectors, host cells, bacterium and plants comprising the recombinant DNA construct or fragments thereof are provided. Further provided are methods of introducing into a host cell or a plant cell a polynucleotide of interest. The method comprises contacting the host cell with a bacterium competent for the transformation of the host cell, wherein the bacterium comprises a transformation vector comprising a recombinant DNA construct.
    Type: Grant
    Filed: December 18, 2015
    Date of Patent: March 16, 2021
    Assignee: AgBiome, Inc.
    Inventors: Vadim Beilinson, James R. Henriksen
  • Patent number: 9902968
    Abstract: A method of increasing the biomass of a plant that includes planting a plant having plant cells carrying an exogenous gene that encodes a thermophilic restriction enzyme that promotes double-stranded DNA breakage, and growing the plant at least until after true leaf development, wherein the mean biomass of plants having the plant cells carrying the exogenous gene that are grown at least until after true leaf development is increased in comparison with the mean biomass of plants of the same species that do not carry the exogenous gene that are grown for the same amount of time.
    Type: Grant
    Filed: March 10, 2014
    Date of Patent: February 27, 2018
    Assignees: KABUSHIKI KAISHA TOYOTA CHUO KENKYUSHO, THE UNIVERSITY OF TOKYO
    Inventors: Nobuhiko Muramoto, Hiroki Sugimoto, Norihiro Mitsukawa, Kunihiro Ohta, Kazuto Kugou
  • Patent number: 9598707
    Abstract: A method to increase the production of products of interest in plant material including plant cultures, such as, for example, cell suspension cultures, root cultures, and hairy root cultures is provided. In one embodiment, the method is to contacting the plant material with a precursor or xenobiotic when producing a product of interest from a plant. In another embodiment the plant material is also contacted with a trapping agent. The process may also provide for contacting an elicitor of the product of interest with the plant material. An embodiment provides for contacting an elicitor, precursor and trapping agent with the plant material. The ability to produce novel compounds such as glucosides and glucuronides is provided.
    Type: Grant
    Filed: March 5, 2013
    Date of Patent: March 21, 2017
    Assignee: Arkansas State University-Jonesboro
    Inventors: Luis Fabricio Medina-Bolivar, Tianhong Yang
  • Patent number: 9447421
    Abstract: The present invention provides methods for Agrobacterium-mediated transformation of sugar cane (Saccharum spp.) comprising introducing a nucleotide sequence of interest into a sugar cane callus tissue or cell thereof via Agrobacterium mediated delivery, wherein the sugar cane callus tissue is less than 28 days post-initiation. The invention further provides methods for transforming a sugar cane callus tissue or cell thereof comprising inoculating the sugar cane callus tissue that is less than 28 days post-initiation with an Agrobacterium comprising a nucleotide sequence of interest to produce an Agrobacterium-inoculated sugar cane tissue or cell thereof; and co-cultivating the Agrobacterium and the sugar cane callus tissue to produce a transformed sugar cane callus tissue or cell thereof.
    Type: Grant
    Filed: June 22, 2011
    Date of Patent: September 20, 2016
    Assignees: Syngenta Participations AG, Queensland University of Technology
    Inventors: Robert Jason Christopher Geijskes, Paulo Cezar De Lucca
  • Patent number: 9228192
    Abstract: A process of producing transgenic plants or plant cells stably transformed on a chromosome with a DNA sequence of interest capable of expressing a function of interest, said process comprising (a) providing plant cells or plants with at least two different vectors that are adapted to recombine with each other between site-specific recombination sites compatible with a site-specific recombinase that is also provided in order to produce a non-replicating recombination product containing said DNA sequence of interest, (ii) said at least two different vectors are adapted for integrating said DNA sequence of interest into said chromosome, (iii) said DNA sequence of interest contains sequence portions from at least two of said at least two different vectors, said sequence portions being necessary for expressing said function of interest from said DNA sequence of interest; and (b) selecting plants or plant cells expressing said function of interest.
    Type: Grant
    Filed: January 30, 2004
    Date of Patent: January 5, 2016
    Assignee: BAYER CROPSCIENCE N.V.
    Inventors: Anatoly Giritch, Serik Eliby, Sylvestre Marillonnet, Victor Klimyuk, Yuri Gleba
  • Patent number: 9095550
    Abstract: The present invention relates to a method of treating chronic wounds using calreticulin. In particular, the invention relates to the treatment of chronic diabetic wounds using topical application of calreticulin to a patient in need of such treatment.
    Type: Grant
    Filed: February 6, 2012
    Date of Patent: August 4, 2015
    Assignees: NEW YORK UNIVERSITY, CALREGEN INC.
    Inventors: Leslie I. Gold, Marek Michalak
  • Patent number: 9045759
    Abstract: The present invention relates to the production of covalently closed circular (ccc) recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly to vector modifications that improve production yield of said DNA molecules in fermentation culture.
    Type: Grant
    Filed: January 19, 2010
    Date of Patent: June 2, 2015
    Inventor: James Arthur Williams
  • Publication number: 20150143587
    Abstract: A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems.
    Type: Application
    Filed: January 16, 2015
    Publication date: May 21, 2015
    Inventors: Anisha Akula, David R. Duncan, Brenda A. Lowe, Michael T. Mann, William L. Petersen, Jyoti R. Rout, David D. Songstad, Joel B. Wilks, Wanggen Zhang
  • Patent number: 8993839
    Abstract: Described herein are viral amplicon-based protein expression systems and methods useful for producing heterologous proteins, such as enzymes, by agroinfiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a heterologous protein, infecting plant cells with the Agrobacterium, allowing expression of the heterologous protein, and recovering the heterologous protein from the plant cells. In one embodiment, the protein produced is an endoglucanase.
    Type: Grant
    Filed: July 19, 2011
    Date of Patent: March 31, 2015
    Assignee: The Regents of the University of California
    Inventors: Minsook Hwang, Benjamin E. Lindenmuth, Karen A. McDonald, Abhaya M. Dandekar, Bryce W. Falk, Sang-Kyu Jung, Nathaniel J. Kingsbury
  • Patent number: 8987554
    Abstract: A method is disclosed for reducing the level of gossypol in cottonseed. The method generally includes selectively inducing RNA gene silencing in the seed of a transgenic cotton plant, to interfere with expression of the ?-cadinene synthase gene or the ?-cadinene-8-hydroxylase gene in the seed of the cotton plant without substantially affecting expression of that gene in the foliage, floral parts, and roots of the plant. The transgenic cotton plant comprises at least one of a ?-cadinene synthase gene trigger sequence and/or a ?-cadinene-8-hydroxylase gene trigger sequence operably linked to one or more a seed-specific promoter gene sequences, and the trigger sequence(s) is/are able to induce RNA gene silencing when expressed in cottonseed of the plant.
    Type: Grant
    Filed: May 17, 2011
    Date of Patent: March 24, 2015
    Assignee: The Texas A&M University System
    Inventors: Keerti S. Rathore, Ganesan Sunilkumar, LeAnne M. Campbell
  • Publication number: 20150082478
    Abstract: Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed.
    Type: Application
    Filed: August 20, 2014
    Publication date: March 19, 2015
    Inventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, L. Aleksander Lyznik, Jinrui Shi, Sergei Svitashev, Joshua K. Young
  • Publication number: 20150067922
    Abstract: The present invention provides compositions and methods for specific gene targeting and precise editing of DNA sequences in plant genomes using the CRISPR (cluster regularly interspaced short palindromic repeats) associated nuclease. Non-transgenic, genetically modified crops can be produced using these compositions and methods.
    Type: Application
    Filed: May 30, 2014
    Publication date: March 5, 2015
    Applicant: The Penn State Research Foundation
    Inventors: Yinong Yang, Kabin Xie
  • Patent number: 8936937
    Abstract: Modified expression vectors, including Tobacco Mosaic Virus (TMV) expression vectors, methods for modifying such vectors, and uses of the same are disclosed.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: January 20, 2015
    Assignee: The Ohio State University Research Foundation
    Inventor: John A. Lindbo
  • Publication number: 20150013031
    Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
    Type: Application
    Filed: June 3, 2014
    Publication date: January 8, 2015
    Inventors: Larry A. Gilbertson, Xudong Ye
  • Patent number: 8921655
    Abstract: The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).
    Type: Grant
    Filed: December 16, 2009
    Date of Patent: December 30, 2014
    Assignee: Joil (S) Pte Ltd.
    Inventors: Jian Ye, Nam Hai Chua, Jing Qu
  • Publication number: 20140366223
    Abstract: This invention provides a method for generating transgenic plants with a low copy number. Plant cells are transformed with polynucleotides containing transcriptional cassettes designed to trigger silencing of a gene which is essential for the plant cell to survive the transformation and regeneration process. The present invention enables the recovery of an increased number of transgenic plants which have only one copy of each desired transcriptional cassette.
    Type: Application
    Filed: June 6, 2014
    Publication date: December 11, 2014
    Applicant: Syngenta Participations AG
    Inventors: Xianggan Li, Sivamani Elumalai
  • Patent number: 8901372
    Abstract: This invention relates to a nucleic acid construct. The construct includes a nucleic acid molecule configured to silence Banana bunchy top virus (BBTV), a 5? DNA promoter sequence, and a 3? terminator sequence. The nucleic acid molecule, the promoter, and the terminator are operatively coupled to permit transcription of the nucleic acid molecule. The present invention also relates to expression vectors, host cells, and transgenic plants containing the nucleic acid construct of the present invention. Also disclosed are methods of imparting BBTV resistance to plants.
    Type: Grant
    Filed: February 25, 2010
    Date of Patent: December 2, 2014
    Assignee: University of Hawaii
    Inventors: John Hu, Wayne Borth, Eden A. Perez, Kheng Cheah
  • Patent number: 8895808
    Abstract: Methods of, and compositions for, assembling one or more transcription units in a genome without a linked selectable marker or other unwanted transcription unit are provided. Also provided methods of, and compositions for, assembling one or more transcription units in a genome with a reduced frequency of vector backbone.
    Type: Grant
    Filed: December 14, 2012
    Date of Patent: November 25, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Susan J. Johnson
  • Patent number: 8889949
    Abstract: The present invention relates to a method for increasing resistance of monocot plants against abiotic stress which comprises a step of transforming monocot plants with a recombinant plasmid containing a fused gene (TPSP) of trehalose-6-phosphate synthetase (TPS) gene and trehalose-6-phosphate phosphatase (TPP) gene to express the TPSP gene while maintaining normal growth and development characteristics. The present invention can increase the resistance of monocot plants against various stresses so that it can greatly contribute to the improvement of production and quality of valuable agricultural crops. The present invention also relates to a transgenic monocot plant, plant cell, or protoplast transformed with a nucleic acid encoding an enzyme for trehalose biosynthesis, under control of an inducible promoter, that increases tolerance to low temperature, salt, and water stress.
    Type: Grant
    Filed: January 3, 2007
    Date of Patent: November 18, 2014
    Assignees: Cornell Research Foundation, Inc., Myongji University Industry and Academia Cooperation Foundation
    Inventors: Ray J. Wu, Ajay K. Garg, Ju-Kon Kim, Baek-Hie Nahm, Yang-Do Choi, In-Cheol Jang, Won-Bin Choi, Yeon-Seak Kim, Chung-Ho Kim, Sang-Ik Song
  • Patent number: 8871997
    Abstract: Disclosed are a variety of methods for achieving enhanced expression from a target nucleotide sequence in a plant e.g. comprising the step of transiently introducing into a tissue of a plant (e.g. a leaf) a first nucleic acid comprising the target nucleotide sequence and a second nucleic acid encoding a Post Transcriptional Gene Silencing (PTGS) suppressor protein (preferably of viral or plant origin), wherein the first and second nucleic acids are comprised within a single binary vector, construct, or the first and second nucleic acid sequences are comprised within a first binary vector and a second binary vector construct respectively. The plant tissue may then be harvested for the protein. Such methods can give much higher levels of gene expression than are obtainable using stable transgenes, or certain replicating vectors.
    Type: Grant
    Filed: November 20, 2006
    Date of Patent: October 28, 2014
    Assignee: Plant Bioscience Limited
    Inventors: David Charles Baulcombe, Olivier Voinnet, Andrew John Hamilton
  • Patent number: 8865969
    Abstract: According to the present invention, a technique of increasing the frequency of genetic recombination in genomic DNA of a plant is established. Such technique comprises: introducing a restriction enzyme gene that can be expressed in a target plant cell into the plant cell and causing the restriction enzyme gene to be transiently expressed so as to induce genetic recombination of genomic DNA; or introducing a promoter and a restriction enzyme gene using the Agrobacterium method so as to induce genetic recombination of genomic DNA.
    Type: Grant
    Filed: January 18, 2011
    Date of Patent: October 21, 2014
    Assignees: Toyota Jidosha Kabushiki Kaisha, Riken
    Inventors: Satoshi Kondo, Chikara Ohto, Kunihiro Ohta, Shuichi Ohsato, Norihiro Mitsukawa, Nobuhiko Muramoto, Hiroki Sugimoto
  • Patent number: 8853488
    Abstract: The present invention provides methods for transforming monocot plants via a simple and rapid protocol, to obtain regenerated plants capable of being planted to soil in as little as 4-8 weeks. Associated cell culture media and growth conditions are also provided, as well as plants and plant parts obtained by the method. Further, a method for screening recalcitrant plant genotypes for transformability by the methods of the present invention is also provided. Further, a system for expanding priority development window for producing transgenic plants by the methods of the present invention is also provided.
    Type: Grant
    Filed: March 8, 2013
    Date of Patent: October 7, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Jyoti R. Rout, Brenda A. Lowe, John Purcell, Anne Spelletich, Michael Spencer, Melissa Way
  • Publication number: 20140273235
    Abstract: Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) systems are provided herein.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Applicant: Regents of the University of Minnesota
    Inventors: Daniel F. Voytas, Paul Atkins, Nicholas J. Baltes
  • Publication number: 20140223607
    Abstract: The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterologous recombinant products including oil and protein products and assessing and screening the efficacy of plant transformation. Also disclosed are transgenic Camelina sativa plants, seeds as well as cells, cell-lines and tissue of Camelina sativa.
    Type: Application
    Filed: February 5, 2014
    Publication date: August 7, 2014
    Applicant: AGRAGEN, LLC
    Inventors: Viktor KUVSHINOV, Anne KANERVA, Kimmo KOIVU, Eija PEHU, Svetlana KUVSHINOVA
  • Patent number: 8759616
    Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.
    Type: Grant
    Filed: March 19, 2013
    Date of Patent: June 24, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Larry A. Gilbertson, Xudong Ye
  • Publication number: 20140173770
    Abstract: Methods and means are provided to modify in a targeted manner the genome of a cotton plant using a double stranded DNA break inducing enzyme and embryogenic callus.
    Type: Application
    Filed: May 30, 2012
    Publication date: June 19, 2014
    Applicant: Bayer CropScience NV
    Inventor: Katelijn D'Halluin
  • Patent number: 8742202
    Abstract: Methods for the transformation of sugar cane are provided. The methods comprise utilizing sugar cane immature shoots as the source of plant material for transformation. Segments of the immature shoot are excised and transformed by any suitable transformation methodology. In some embodiments, the segments are cultured in embryogenic culture induction medium prior to transformation. Transformation can be performed via Agrobacterium-mediated gene delivery, biolistic transformation, and the like. Transgenic plants are regenerated from plantlets grown under conditions favoring growth of transformed cells while substantially inhibiting growth of non-transformed cells.
    Type: Grant
    Filed: December 1, 2009
    Date of Patent: June 3, 2014
    Assignee: Syngenta Participations AG
    Inventor: Heng Zhong
  • Patent number: 8697949
    Abstract: This invention provides molecular constructs and methods for the temporally specific control of gene expression in plants or in plant pests or pathogens. More specifically, this invention provides plant miRNA genes having novel circadian expression patterns that are useful for designing recombinant DNA constructs for temporally specific expression of at least one gene. Also provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention.
    Type: Grant
    Filed: June 26, 2008
    Date of Patent: April 15, 2014
    Assignee: Monsanto Technology LLC
    Inventors: Edwards Allen, Sara E. Heisel, Sergey Ivashuta, Elysia Krieger, Jennifer Lutke, Robert J. Meister, Yuanji Zhang
  • Patent number: 8686223
    Abstract: The present invention relates to methods for the regeneration and Agrobacterium-mediated transformation of plants in the genera of Jatropha, more specifically, in Jatropha curcas.
    Type: Grant
    Filed: July 3, 2012
    Date of Patent: April 1, 2014
    Assignee: Joil (S) Pte Ltd.
    Inventors: Hui Zhu Mao, Jian Ye, Nam Hai Chua
  • Publication number: 20140082767
    Abstract: A transgenic plant includes a first gene having a first polynucleotide and a recombinant construct having a second polynucleotide. The first polynucleotide is homologous to a Atclb gene fragment represented by nucleotide acids 963-1205 of SEQ ID NO:1. The first polynucleotide encodes a C2-like domain, and the Atclb gene fragment encodes a C2 domain represented by amino acids 265-345 of SEQ ID NO:2. The C2-like domain is homologous to the C2 domain. The second polynucleotide has at least 90% identity to the first polynucleotide or a sequence complimentary to the first polynucleotide. The recombinant construct is configured to silence the first gene such that the transgenic plant is tolerant to abiotic stress. A method to produce the transgenic plants.
    Type: Application
    Filed: September 18, 2013
    Publication date: March 20, 2014
    Applicant: BOARD OF TRUSTEES OF THE UNIVERSITY OF ARKANSAS
    Inventor: Mariya Khodakovskaya
  • Patent number: 8674178
    Abstract: Described herein are methods useful for producing proteins, such as enzymes, by agrofiltration. The methods involve producing an Agrobacterium with a Ti plasmid encoding a cellulase, infecting plant cells with the Agrobacterium, allowing expression of the cellulase, and recovering the cellulase from the plant cells. In one embodiment, the protein produced is an endoglucanase.
    Type: Grant
    Filed: August 19, 2009
    Date of Patent: March 18, 2014
    Assignee: The Regents of the University of California
    Inventors: Karen A. McDonald, Benjamin E. Lindenmuth, Abhaya M. Dandekar, Bryce W. Falk
  • Patent number: 8669417
    Abstract: The invention provides methods and compositions for increasing the efficiency of genetic transformation of host cells, including plant cells, and other eukaryotic cells, by reducing the expression of a polypeptide active in a pathway, such as the NHEJ pathway, for repairing damage to the cellular genome. In certain embodiments, the polypeptide is active in repairing double strand breaks (DSB's) of a cellular genome, and may include XRCC4, KU70, KU80, the DNA-activated Protein Kinase (DNA-Pkcs), and ATM. Methods for enhancing the resistance of plant cells to Crown Gall disease are also provided. In another aspect, genetic regulatory elements are provided, including an XRCC4 promoter.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: March 11, 2014
    Assignee: The Samuel Roberts Noble Foundation, Inc.
    Inventors: Zarir E. Vaghchhipawala, Kirankumar Mysore
  • Patent number: 8642839
    Abstract: Compositions and methods for transiently expressing proteins in a plant are provided. The compositions comprise plants, seeds, plant tissues, and plant parts expressing a protein, wherein the protein is expressed transiently and the transient expression of the protein can be used as a predictive model of how said protein will be expressed in stable transgenic plants in regards to qualitative and quantitative data. The predictive model may be used but is not limited to: promoter evaluation, evaluation of expression cassette construction for best performance (e.g. addition of enhancers or gene silencing suppressors), evaluation of best ways to express heterologous genes (e.g. point mutations, targeting), fast evaluation of endogenous gene knockout, evaluation of protein expression levels, cellular targeting, tissue targeting, transcriptional enhancers, translational enhancer protein toxicity and metabolic profiling. Further provided are methods of use.
    Type: Grant
    Filed: June 11, 2010
    Date of Patent: February 4, 2014
    Assignee: Syngenta Participations AG
    Inventor: Kasimalai Azhakanandam
  • Patent number: 8617575
    Abstract: Immunity against protozoan is conferred on an animal by a method comprising orally administering to an animal a transformed plant cell comprising a polynucleotide encoding a protective antigen against protozoiasis development, a transformed plant or its progeny or clone comprising the transformed cell, a propagation material of the plant or its progeny or clone, a processed material or extract of the above cell, plant, or its progeny or clone, or propagation material, or a protective antigen against protozoiasis development isolated from the transformed plant cell or the transformed plant or its progeny or clone.
    Type: Grant
    Filed: May 30, 2005
    Date of Patent: December 31, 2013
    Assignees: Kitasato Daiichi Sankyo Vaccine Co., Ltd., National Institute of Advanced Industrial Science and Technology
    Inventors: Akira Ito, Toru Gotanda, Shigeki Kobayashi, Katsumi Kume, Takeshi Matsumura
  • Patent number: 8609934
    Abstract: Methods of providing gene suppression DNA in a eukaryotic organism comprising introducing a first DNA segment and at least one second DNA segment into the genome of the organism. One of the DNA segments contains a promoter and a transcribable DNA. Another DNA segment contains at least part of the transcribable DNA. When inserted in tandem, the DNA segments are assembled in vivo forming a recombinant transcription unit. RNA transcribed from the transcription unit can form double-stranded RNA.
    Type: Grant
    Filed: February 11, 2005
    Date of Patent: December 17, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Joanne Fillatti, Larry Gilbertson, Toni Voelker
  • Patent number: 8592212
    Abstract: A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced directly to form shoots that give rise to transgenic plants. This method does not involve callus-phase tissue culture and is rapid and efficient.
    Type: Grant
    Filed: September 26, 2011
    Date of Patent: November 26, 2013
    Assignee: Monsanto Technology LLC
    Inventors: Brian J. Martinell, Lori S. Julson, Carol A. Emler, Yong Huang, Dennis E. McCabe, Edward J. Williams
  • Patent number: 8581035
    Abstract: The invention provides methods for identifying regenerated transformed plants and differentiated transformed plant parts, obtained without subjecting plant cells to selective conditions prior to regenerating the cells to obtain differentiated tissues. In particular embodiments, the plant cells are corn plant cells. Methods for growing and handling plants, including identifying plants that demonstrate specific traits of interest are also provided.
    Type: Grant
    Filed: August 31, 2007
    Date of Patent: November 12, 2013
    Assignee: Monsanto Technology LLC
    Inventor: Jyoti R. Rout
  • Patent number: 8581045
    Abstract: This disclosure relates to a mutant Agrobacterium tumefaciens that is functionally deleted for the atu1060 gene that codes for the cyclic di-GMP synthase Atu1060, as well as methods for its use in transforming plants with desired transgenes. Such bacteria are more virulent than currently used strains of A. tumefaciens, and thus can be used to transform a wider variety of plants, such as plants that are traditionally recalcitrant to such transformation.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: November 12, 2013
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Stephen K. Farrand, David Michael Barnhart
  • Publication number: 20130291224
    Abstract: Transgenic plants having increased growth rate, increased sugar content, and increase yield are disclosed, and methods for making the same. The transgenic plants have a gene coding for a phosphatase having a C-terminal motif under control of a heterologous promoter incorporated into the genomic DNA of the plant.
    Type: Application
    Filed: April 15, 2013
    Publication date: October 31, 2013
    Inventor: Boon Leong Lim
  • Publication number: 20130254943
    Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.
    Type: Application
    Filed: November 12, 2012
    Publication date: September 26, 2013
    Applicant: DOW AGROSCIENCES LLC
    Inventors: Sandeep Kumar, Diaa Alabed, Terry Wright, Manju Gupta
  • Patent number: 8541653
    Abstract: The present invention relates to improved methods for the incorporation of DNA into the genome of a soybean (Glycine max) plant utilizing meristematic cells of primary or higher leaf nodes as target tissue by means of Agrobacterium-mediated transformation and subsequent regeneration of the transformed cells into a whole plant.
    Type: Grant
    Filed: June 4, 2005
    Date of Patent: September 24, 2013
    Assignee: BASF Plant Science GmbH
    Inventors: Paula Olhoft, Leslie Grist, Libby Bernal, Sara Price, Diana Arias, Haiping Hong
  • Publication number: 20130247252
    Abstract: The present disclosure relates, according to some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant (e.g., a monocot) using a promoter operable in one or more plant tissues and/or cells. In some embodiments, an isolated nucleic acid may comprise an expression control sequence having the sequence of nucleotides 1-4726 of SEQ ID NO: 1, wherein the expression control sequence has stem-regulated and/or defense-inducible promoter activity in at least one monocot (e.g., at least two monocots).
    Type: Application
    Filed: March 13, 2013
    Publication date: September 19, 2013
    Applicant: THE TEXAS A&M UNIVERSITY SYSTEM
    Inventors: Mona B. DAMAJ, T. Erik MIRKOV, Terry L. THOMAS, Keerti S. RATHORE, Chandrakanth EMANI, Siva P. KUMPATLA
  • Patent number: 8519227
    Abstract: There is provided an Agrobacterium transformation method for monocotyledons, comprising a step of infecting an intact seed.
    Type: Grant
    Filed: November 2, 2004
    Date of Patent: August 27, 2013
    Inventors: Hiroshi Tanaka, Toshiaki Kayano, Masashi Ugaki, Fumio Shiobara, Naoto Shibuya, Haruko Onodera, Kazuko Ono, Akemi Tagiri, Yaeko Nishizawa
  • Patent number: 8507220
    Abstract: An expression vector is constructed by transferring recombinant tomato mosaic virus (ToMV) cDNA, in which a coat protein gene of ToMV having a suppressor against a virus resistant reaction has been substituted by a GFP gene, into the downstream of a promoter capable of inducing steroid hormone-dependent transcription. In a transformed tobacco BY-2 cell obtained by transferring the above expression vector into a tobacco BY-2 cells, steroid hormone-dependent transcription is induced, thereby enabling the amplification of mRNA of the GFP gene and induction of the expression of GFP.
    Type: Grant
    Filed: October 1, 2004
    Date of Patent: August 13, 2013
    Assignee: Japan Science and Technology Agency
    Inventors: Masashi Mori, Koji Dohi, Masayuki Ishikawa, Tetsuo Meshi, Masaki Nishikiori, Atsushi Tamai
  • Publication number: 20130157369
    Abstract: Methods to increase transformation frequency in plants when using Agrobacterium as the transformant are described. The methods include exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant. Some methods include exposing the plant cells to continuous light after exposure to the Agrobacterium cells. Examples of plants useful with these methods include maize plants (e.g., immature maize embryos).
    Type: Application
    Filed: December 14, 2012
    Publication date: June 20, 2013
    Applicant: DOW AGROSCIENCES LLC
    Inventor: DOW AGROSCIENCES LLC
  • Publication number: 20130152232
    Abstract: The present invention provides methods for Agrobacterium-mediated transformation of sugar cane (Saccharum spp.) comprising introducing a nucleotide sequence of interest into a sugar cane callus tissue or cell thereof via Agrobacterium mediated delivery, wherein the sugar cane callus tissue is less than 28 days post-initiation. The invention further provides methods for transforming a sugar cane callus tissue or cell thereof comprising inoculating the sugar cane callus tissue that is less than 28 days post-initiation with an Agrobacterium comprising a nucleotide sequence of interest to produce an Agrobacterium-inoculated sugar cane tissue or cell thereof; and co-cultivating the Agrobacterium and the sugar cane callus tissue to produce a transformed sugar cane callus tissue or cell thereof.
    Type: Application
    Filed: June 22, 2011
    Publication date: June 13, 2013
    Applicant: Synegenta Participatins AG
    Inventors: Robert Jason Christopher Geijskes, Paulo Cezar De Lucca
  • Publication number: 20130133109
    Abstract: The present disclosure relates to the development and use of Closterovirus-based vectors for the delivery of nucleotides to plants. Specifically, the present disclosure provides viral vectors based on Grapevine leafroll-associated virus-2 for the delivery and expression of genes in plants, particularly grape plants. Methods of making and using these vectors, as well as the plants transformed by these vectors, are also contemplated.
    Type: Application
    Filed: January 29, 2013
    Publication date: May 23, 2013
    Applicants: State University
    Inventor: State of Oregon Acting by and through the State Board of Higher Education on behalf of Oregon State University
  • Patent number: RE44750
    Abstract: The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformation.
    Type: Grant
    Filed: September 10, 2012
    Date of Patent: February 4, 2014
    Assignee: BASF Plant Science GmbH
    Inventors: Luke Mankin, Dwight-Steven Hill, Paula Olhoft, Effie Toren, Jeffrey A. Brown, Liqun Xing, Huihua Fu, Lesley Ireland, Hongmei Jia, Hee-Sook Song, Allan R. Wenck, Larry Nea