Involving Virus Or Bacteriophage Patents (Class 435/5)
  • Patent number: 10352930
    Abstract: The present invention provides an immunoassay leading to the rapid and simultaneous detection of antibodies to a wide range of infectious pathogens in biological fluids of infected patients. This immunoassay involves the covalent and oriented coupling of fusion proteins comprising an AGT enzyme and a viral antigen on an identifiable solid support (e.g. fluorescent microspheres). The thus obtained antigen-coupled microspheres show enhanced capture of specific antibodies as compared to antigen-coupled microspheres produced by standard amine coupling procedures.
    Type: Grant
    Filed: July 19, 2017
    Date of Patent: July 16, 2019
    Assignee: INSTITUT PASTEUR
    Inventors: Jean-Claude Manuguerra, Jessica Vanhomwegen, Philippe Despres, Sylvie Paulous
  • Patent number: 10352937
    Abstract: The present invention relates to a pretreatment method of a sample for detecting HBs antigen, which is a surface antigen of the hepatitis B virus, and a method for detecting HBs antigen utilizing the pretreatment method. The present invention also relates to a pretreatment reagent kit for detecting HBs antigen.
    Type: Grant
    Filed: July 27, 2015
    Date of Patent: July 16, 2019
    Assignee: SYSMEX CORPORATION
    Inventors: Takahiro Yamagaito, Takuya Koma, Mari Maruki, Kazuhiko Takeda
  • Patent number: 10351909
    Abstract: The invention relates to an automated method for high-throughput DNA sequencing from high density DNA arrays by (a) initiating a first sequencing reaction on a first high density DNA array; and imaging said first high density DNA array using a detector, and (b) initiating a first sequencing reaction on a second high density DNA array; and imaging said second high density DNA array using the detector, wherein the first sequencing reaction in (a) is initiated before the first sequencing reaction in (b) is initiated such that the sequencing reactions in (a) and (b) are staggered. By using asynchronous sequencing reactions and imaging two separate arrays using one detector, imaging can be carried out on one array while sequencing reactions are carried out on one the other, substrate, the other substrate is imaged, reducing the idle time of the imaging system.
    Type: Grant
    Filed: February 25, 2017
    Date of Patent: July 16, 2019
    Assignee: Complete Genomics, Inc.
    Inventors: Radoje Drmanac, Matthew J. Callow, Snezana Drmanac, Brian K. Hauser, George Yeung
  • Patent number: 10351912
    Abstract: Methods of using probes and probe sets for the detection of high grade dysplasia and carcinoma in cervical cells are described. Methods of the invention include hybridizing one or more chromosomal probes to a biological sample obtained from a subject and detecting the hybridization pattern of the chromosomal probes to the sample to determine whether the subject has high grade dysplasia or carcinoma. Methods of the invention also include preliminary screening the cells for a marker associated with a risk for cancer, and preferably involves screening for HPV infected cells by in situ hybridization using an HPV probe mixture.
    Type: Grant
    Filed: October 24, 2011
    Date of Patent: July 16, 2019
    Assignee: Abbott Molecular Inc.
    Inventors: Irina A. Sokolova, Steven A. Seelig, Larry E. Morrison, Walter King, Alicia Algeciras-Schimnich
  • Patent number: 10345308
    Abstract: Anti-carbohydrate antibodies are detected by (a) contacting an array of oligomannose-serum albumin conjugates immobilized on a substrate with an antibody-containing serum sample under conditions wherein TM10 antibodies bind the oligomannose of the conjugates at at least micromolar affinity; and (b) detecting resultant binding of specific antibodies of the sample to the oligomannose of the conjugates, as indicative of the anti-carbohydrate antibodies.
    Type: Grant
    Filed: October 23, 2014
    Date of Patent: July 9, 2019
    Assignee: SRI INTERNATIONAL
    Inventor: Denong Wang
  • Patent number: 10342861
    Abstract: The invention relates to compositions and methods for preventing or treating arenavirus related diseases and disorders through the administration to a subject in need thereof a live-attenuated virus (LAV), wherein the LAV is a codon deoptimized (CD) arenavirus.
    Type: Grant
    Filed: May 27, 2015
    Date of Patent: July 9, 2019
    Assignees: UNIVERSITY OF ROCHESTER, THE SCRIPPS RESEARCH INSTITUTE
    Inventors: Luis Martinez-Sobrido, Juan Carlos De La Torre
  • Patent number: 10335480
    Abstract: The present specification discloses recombinant nucleic acid constructs encoding an immunogenic multiepitope polypeptide comprising two or more polypeptides, recombinant nucleic acid constructs encoding at least two epitopes from two or more internal proteins of influenza virus, compositions comprising such recombinant nucleic acid constructs and methods of eliciting a T cell immune response against an influenza virus in a vertebrate using such recombinant nucleic acid constructs and compositions.
    Type: Grant
    Filed: January 22, 2018
    Date of Patent: July 2, 2019
    Assignee: PepTcell Limited
    Inventors: Gregory A. Stoloff, Wilson Romero Caparros-Wanderley
  • Patent number: 10329538
    Abstract: Attenuated isolates of the Arkansas serotype of infectious bronchitis virus (IBV), including the IBV isolate ArkGA p60 deposited at the ATCC under Patent Designation PTA-123783, and compositions thereof are presented. Methods for administering the isolates or compositions as vaccines to the prevent virulent IBV infection in birds of the order Galliformes are also presented.
    Type: Grant
    Filed: January 26, 2018
    Date of Patent: June 25, 2019
    Assignee: UNIVERSITY OF GEORGIA RESEARCH FOUNDATION, INC.
    Inventors: Brian J. Jordan, Mark W. Jackwood
  • Patent number: 10329603
    Abstract: This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes.
    Type: Grant
    Filed: April 25, 2017
    Date of Patent: June 25, 2019
    Assignee: ALERE SAN DIEGO INC.
    Inventors: Olaf Piepenburg, Colin H. Williams, Niall A. Armes, Derek L. Stemple
  • Patent number: 10316311
    Abstract: This disclosure provides antibody-urease conjugates having therapeutic and diagnostic utility. More specifically, the disclosure relates to diagnostic and/or therapeutic conjugates that are prepared by conjugating one or more whole antibodies to urease.
    Type: Grant
    Filed: April 3, 2014
    Date of Patent: June 11, 2019
    Assignee: Helix Biopharma Corp.
    Inventor: Heman Chao
  • Patent number: 10316364
    Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: June 11, 2019
    Assignee: KEYGENE N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Taco Peter Jesse, Adrianus Johannes Van Tunen
  • Patent number: 10308990
    Abstract: Methods of evaluating a cellular sample for latent cellular replication competent HIV-1 are provided. Aspects of the methods include contacting a cellular sample with an HIV-1 inducing compound to produce an activated cellular sample; and assessing plasma viral load in the activated cellular sample to evaluate the cellular sample for latent cellular replication competent HIV-1. Also provided are devices and kits that find use in practicing the methods.
    Type: Grant
    Filed: July 23, 2015
    Date of Patent: June 4, 2019
    Assignee: IncellDx, Inc.
    Inventors: Bruce K. Patterson, Amanda Noel Chargin, Fangfang Yin, Min Song, Srividyabhuvaneswari Subramaniam, Grace Elaine Knutson
  • Patent number: 10308989
    Abstract: The instant disclosure provides norovirus binding aptamers, compositions comprising such aptamers, and methods of using and producing such aptamers. The aptamers are useful, for example, for detecting the presence of norovirus in test samples, for capturing and/or concentrating norovirus from test samples, for evaluating the efficacy of therapeutic agents in patients diagnosed with a norovirus infection, and for evaluating the efficacy of norovirus vaccines.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: June 4, 2019
    Assignee: NORTH CAROLINA STATE UNIVERSITY
    Inventors: Lee-Ann Jaykus, Blanca Irene Escudero-Abarca, Matthew D. Moore, Helen Rawsthorne
  • Patent number: 10309964
    Abstract: The invention belongs to the fields of medicine and immunology, particularly, the field of immunological diagnosis. In particular, the invention discloses a method for assessing whether a subject is at risk of developing human cytomegalovirus (HCMV) active infection and a kit therefore. The method comprises the steps of: (1) determining the level of an antibody against a HCMV protein in a body fluid sample from the subject; and (2) comparing the level with a predetermined reference value, wherein if the level is below the predetermined reference value, the subject is determined to be at risk of developing HCMV active infection. In addition, the invention also discloses a method for screening a candidate drug which is capable of improving the ability of a subject to resist human cytomegalovirus (HCMV) active infection, and a kit therefore.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: June 4, 2019
    Assignee: Xiamen University
    Inventors: Shengxiang Ge, Jinjie Li, Xi Huang, Tingdong Li, Jun Zhang, Ningshao Xia
  • Patent number: 10301601
    Abstract: The present invention discloses a method for terminal inactivation of pathogenic microorganisms, comprising the following steps of: a. vacuum freeze-drying a bio-product packaged in a container, feeding in a gas and sealing to obtain the end product and, b. performing inactivation by dry-heating. The method provided by the present invention can effectively inactivate non-lipid enveloped viruses, with excellent inactivation effects and short inactivation time particularly to parvoviruses, and thus overcome the deficiencies of the conventional terminal dry-heating inactivation methods.
    Type: Grant
    Filed: December 22, 2014
    Date of Patent: May 28, 2019
    Assignee: Sichuan Yuandashuyang Phamaceutical Co., Ltd
    Inventors: Shuguang Ran, Qiang Wang, Dexi Jiang
  • Patent number: 10301310
    Abstract: The present invention provides quenchers of excited state energy, probes and other conjugates comprising these quenchers, and methods of their use.
    Type: Grant
    Filed: May 11, 2015
    Date of Patent: May 28, 2019
    Assignee: BIOSEARCH TECHNOLOGIES, INC.
    Inventors: Mark Reddington, Ronald M. Cook, Ben Ayer
  • Patent number: 10301674
    Abstract: This specification generally relates to non-radioactive methods of detecting nucleic acid polymerase activity and methods of detecting compounds that modulate nucleic acid polymerase activity. The activity may be measured in real-time using a real-time PCR instrument.
    Type: Grant
    Filed: August 19, 2011
    Date of Patent: May 28, 2019
    Assignee: Life Technologies Corporation
    Inventors: Lei Xi, Keith P. Bjornson, Stephen P. Hendricks
  • Patent number: 10300080
    Abstract: A compound having an antiviral activity for inhibiting release of an enveloped virus from a cell is disclosed, including methods of inhibiting release of an enveloped virus from a cell. The antiviral activity of the compound includes inhibiting formation of an associative complex or disrupting formation of an associative complex. The associative complex comprises an L-domain motif of the enveloped virus and at least one cellular polypeptide, or fragment thereof, capable of binding the L-domain motif of the enveloped virus.
    Type: Grant
    Filed: November 10, 2015
    Date of Patent: May 28, 2019
    Assignees: Northwestern University, The Research Foundation for the State University
    Inventors: Jonathan Leis, Carol Carter
  • Patent number: 10300130
    Abstract: Methods of increasing diversity in cytomegalovirus vaccines through the selection of cell type in which the virus is propagated, and the use of cytomegalovirus produced by those methods in the development of vaccine compositions, are disclosed. Vaccine compositions comprising CMV isolated from epithelial cells are also disclosed.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: May 28, 2019
    Assignee: The Trustees of Princeton University
    Inventors: Thomas Shenk, Dai Wang
  • Patent number: 10294531
    Abstract: Disclosed herein are method, materials and kits for detection of B. Burgdorferi infection or determining stage of Lyme disease in a subject. Exemplified is a method of diagnosing an infection in a subject, the method involving exposing a biological sample from the subject to a capture substrate under conditions for an infection marker in said biological sample to associate with the capture substrate to form a capture complex; associating said capture complex with a marker complex, said marker complex comprising an oligonucleotide; and amplifying said oligonucleotide of marker complex associated with said capture complex to produce an amplification signal; wherein an amplification signal above a predetermined signal threshold indicates that said subject is infected.
    Type: Grant
    Filed: October 7, 2014
    Date of Patent: May 21, 2019
    Assignee: UNIVERSITY OF CENTRAL FLORIDA RESEARCH FOUNDATION, INC.
    Inventors: Mollie Jewett, Micah Halpern
  • Patent number: 10294516
    Abstract: Methods for enhancing the binding of oligonucleotide probes to DNA and RNA are disclosed. The methods make use of thermodynamic and kinetic effects to reduce probe mismatches and failure of complementary probes to bind to DNA and RNA templates. Mapping and sequencing of the probed DNA and RNA samples are contemplated herein.
    Type: Grant
    Filed: January 16, 2014
    Date of Patent: May 21, 2019
    Assignee: NABSYS 2.0 LLC
    Inventor: John S. Oliver
  • Patent number: 10286377
    Abstract: There is disclosed an electrode array device having an adsorbed porous reaction layer for improved synthesis quality. The array comprises a plurality of electrodes on a substrate, wherein the electrodes are electronically connected to a computer control system. The array has an adsorbed porous reaction layer on the plurality of electrodes, wherein the adsorbed porous reaction layer comprises a chemical species having at least one hydroxyl group. In the preferred embodiment, the reaction layer is sucrose. A method for preparing an electrode array for improved synthesis quality is disclosed. The method comprises a cleaning method and a method of attachment of a reaction layer. The cleaning method comprises a plasma cleaning method and a chemical cleaning method. The reaction layer is attached after cleaning by exposing the microarray to a solution containing the chemical species having at least one hydroxyl group.
    Type: Grant
    Filed: May 15, 2016
    Date of Patent: May 14, 2019
    Assignee: CustomArray, Inc.
    Inventor: Andrei Gindilis
  • Patent number: 10287621
    Abstract: The invention relates to a method which enables the possibility to capture chromosome conformation as well as a kit containing components useful to be used the method, such as to detect promoter and enhancer relations. The method comprises the following steps: i) providing cross-linked genomic DNA, wherein the DNA comprises a first and a second set of regions, ii) fragmenting the cross-linked genome, thus creating a plurality of fragments with junctions, iii) adding a labelled junction marker such as biotin and ligating the fragments to the marker, iv) purifying the marked fragments, v) adding labelled capture probes and selectively purifying the hybridized fragments, and vi) analyzing the fragments captured by hybridization and identify the fragments.
    Type: Grant
    Filed: April 11, 2014
    Date of Patent: May 14, 2019
    Assignee: PELIN SAHLÉN
    Inventor: Pelin Sahlén
  • Patent number: 10280455
    Abstract: Methods and compositions are provided for improved nucleic acid amplification assays.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: May 7, 2019
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Nicholas Heredia, Dianna Maar
  • Patent number: 10274443
    Abstract: A urea concentration identification method is provided. By providing an identical sine-wave AC signal to each of the urea concentration identification devices placed in urea solutions of different concentrations, different impedance values are output by the urea concentration identification devices since the urea solutions of different concentrations have different electrical interactions with the electrodes of the urea concentration identification device. Differences of the impedance output by the urea concentration identification device function as a data set for determining the concentration of the urea solution to be determined.
    Type: Grant
    Filed: December 8, 2017
    Date of Patent: April 30, 2019
    Assignee: Industrial Technology Research Institute
    Inventors: Tzu-Yu Liu, Kuo-Chuang Chiu, Hung Tien, Yeh-Chyang Huang
  • Patent number: 10272134
    Abstract: A method of reducing a latent HIV-specific memory-CD4+ T cell pool in a subject includes the steps of: preparing at least one HIV-1 protein coding sequence from a sample obtained from the subject, wherein the sample includes HIV-1 RNA; introducing the at least one HIV-1 protein coding sequence into at least one expression construct using yeast homologous recombination; transfecting a cell with the at least one expression construct, wherein the HIV-1 protein is secreted by the cell and administering a therapeutically effective amount of the secreted HIV-1 protein and a pharmaceutically acceptable carrier to the subject, wherein the secreted HIV-1 protein stimulates latent HIV-specific memory-CD4+ T cells to induce latent HIV-1 replication resulting in HIV-specific memory-CD4+ T cell death in the subject.
    Type: Grant
    Filed: May 8, 2017
    Date of Patent: April 30, 2019
    Assignee: CASE WESTERN RESERVE UNIVERSITY
    Inventor: Eric J. Arts
  • Patent number: 10274414
    Abstract: Systems, methods and non-transitory storage medium are disclosed herein for adjusting an output of a particle inspection system representative of a particle characteristic for a particle flowing in a flow-path of a particle processing system. More particularly, the output may be processed and a calibrated output of the particle characteristic generated. In other embodiments, one or more calibration particles are used. Thus, an output of a particle inspection system representative of a particle characteristic for one or more calibration particles flowing in a flow-path of a particle processing system may be compared relative to a standard and an action may be taken based on a result of the comparing the output to the standard.
    Type: Grant
    Filed: May 19, 2014
    Date of Patent: April 30, 2019
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Emanuel Tito Mendes Machado, Rudolf Hulspas
  • Patent number: 10272410
    Abstract: Provided herein are devices for the manufacturing of high-quality oligonucleic acids. Longer nucleic acids, e.g., genes, can be synthesized in parallel using microfluidic assemblies described herein. Devices described herein include silicon plates having a plurality of channels in fluid communication with a plurality of microchannels. The number of microchannels and dimensions of the microchannels provide for rapid exchange of chemical exposure during de novo synthesis of oligonucleic acids.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: April 30, 2019
    Assignee: Twist Bioscience Corporation
    Inventors: William Banyai, Bill James Peck, Andres Fernandez, Siyuan Chen, Pierre Indermuhle
  • Patent number: 10268800
    Abstract: The present invention relates to methods for evaluating and/or predicting the outcome of a clinical condition, such as cancer, metastasis, AIDS, autism, Alzheimer's, and/or Parkinson's disorder. The methods can also be used to monitor and track changes in a patient's DNA and/or RNA during and following a clinical treatment regime. The methods may also be used to evaluate protein and/or metabolite levels that correlate with such clinical conditions. The methods are also of use to ascertain the probability outcome for a patient's particular prognosis.
    Type: Grant
    Filed: June 27, 2017
    Date of Patent: April 23, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: John Zachary Sanborn, David Haussler
  • Patent number: 10268033
    Abstract: An analysis device for quantifying a state of a fluorescent image containing a plurality of bright spots comprises an area setting unit in which states of a plurality of bright spots contained in a plurality of areas set in the fluorescent image in accordance with positions of the plurality of bright spots are quantified as numerical values.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: April 23, 2019
    Assignee: NIKON CORPORATION
    Inventor: Ichiro Sase
  • Patent number: 10267792
    Abstract: A device for detecting toxic shock syndrome toxins includes a pad and a membrane coupled to the pad. The pad includes first antibodies that include a first particular antibody and a second particular antibody. Each of the first antibodies are associated with toxic shock syndrome toxin one (TSST-1). The first particular antibody is configured to react with a TSST-1 antigen to form an antibody complex. The membrane includes a first zone that includes an immobilized second antibody configured to react with the antibody complex to cause a first indication. The membrane further includes a second zone that includes a third antibody configured to react with the second particular antibody to cause a second indication.
    Type: Grant
    Filed: September 9, 2016
    Date of Patent: April 23, 2019
    Assignee: International Business Machines Corporation
    Inventors: Daniel J. Buvid, Eric J. Campbell, Sarah K. Czaplewski, Christopher W. Steffen
  • Patent number: 10266882
    Abstract: Disclosed are a human rare blood type detection method, a kit, a rapid screening method and applications thereof. By using multiple pairs of PCR specific primers directing to the SNP loci of multiple rare blood types, the SNP loci of multiple rare blood types are simultaneously detected in the same PCR reaction system; and the multiplex PCR detection method and a Pool detection method are combined to rapidly screen the human rare blood types.
    Type: Grant
    Filed: January 18, 2012
    Date of Patent: April 23, 2019
    Assignee: SHANGHAI BLOOD CENTRE
    Inventors: Luyi Ye, Ziyan Zhu, Zhonghui Guo, Yunlei He, Huanhuan Gao, Pan Wang, Li Xie, Aoxue Zhu, Wei Zhang, Wenjie Gao, Qixiu Yang
  • Patent number: 10266568
    Abstract: Rheumatoid arthritis is efficiently diagnosed with improved patient's convenience using a rheumatoid arthritis diagnosis composition and a kit, each including cyclic citrullinated peptide (CCP), a rheumatoid arthritis diagnosis method using the CCP, the rheumatoid arthritis diagnosis composition, or the kit, a method of obtaining information for rheumatoid arthritis diagnosis, and a method of screening a novel diagnostic marker for rheumatoid arthritis.
    Type: Grant
    Filed: September 10, 2015
    Date of Patent: April 23, 2019
    Assignee: Korea Institute of Science and Technology
    Inventors: Min Park, Min-Jung Kang, Byung Hwa Jung, Young Sook Yoo
  • Patent number: 10258991
    Abstract: A detection device of a sample to be examined is described, including a processing module, a detecting module, connected to the processing module, a support, in which the sample to be examined can be placed, the support including a plurality of sensors, preferably biosensors and/or nanosensors, and being insertable within the detecting module, and a containment enclosure including a body, which is arranged within the processing module, and a sensor portion, in which the detecting module is arranged. The processing module includes a processing unit having a plurality of processing channels and a processor, each one of the processing channels being connected to one respective of the sensors and being adapted to amplify and to filter the signals of the sensors, the processor processing the signals amplified and filtered by the processing channels.
    Type: Grant
    Filed: February 13, 2015
    Date of Patent: April 16, 2019
    Assignee: ELEMENTS S.R.L.
    Inventors: Federico Thei, Michele Rossi, Marco Bennati, Francesca Manzoni
  • Patent number: 10258687
    Abstract: The present invention relates to methods for obtaining a whole virus vaccine candidate stock. The present invention also relates to an inactivated whole virus vaccine candidate stock that can be used for vaccination purposes as well as development of novel high titer virus, which is the preferred virus for this technique.
    Type: Grant
    Filed: April 16, 2015
    Date of Patent: April 16, 2019
    Assignee: HVIDOVRE HOSPITAL
    Inventors: Christian Kjærulff Mathiesen, Tanja Bertelsen Jensen, Jens Bukh, Judith Margarete Gottwein
  • Patent number: 10253349
    Abstract: Provided herein are methods and compositions for detecting gene fusions, e.g., relevant to cancer. The present methods and compositions can be used to detect gene fusions with very high sensitivity and specificity. The present methods and compositions can detect gene fusions, e.g., in free circulating tumor RNA from a plasma sample.
    Type: Grant
    Filed: April 15, 2016
    Date of Patent: April 9, 2019
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Ann Begovich, Rajiv Dua, Dwight Kuo, Xiaoju Max Ma, Ellen Ordinario
  • Patent number: 10251948
    Abstract: Provided are antigenic polypeptides of HIV envelope glycoproteins which are constructed based on amino acid mutation of attenuated live vaccine of Equine Infectious Anemia Virus, DNA constructions and recombinant virus vectors comprising polynucleotides encoding said polypeptides, antibodies against said polypeptides as well as uses thereof in preventing and treating HIV infection. Said antigenic polypeptides and vaccines can induce high titer neutralization antibodies against HIV in organism.
    Type: Grant
    Filed: January 21, 2015
    Date of Patent: April 9, 2019
    Assignee: National Center for AIDS/STD Control And Prevention, Chinese Center for Disease Control and Prevention
    Inventors: Yiming Shao, Lianxing Liu, Rongxian Shen
  • Patent number: 10253109
    Abstract: Compositions and methods of using antibodies that are able to recognize single amino acid polymorphisms in a protein are provided. Compositions are disclosed which may be used for blood typing or to block hemolytic transfusion reactions and/or hemolytic disease of the fetus and newborn.
    Type: Grant
    Filed: July 21, 2015
    Date of Patent: April 9, 2019
    Assignee: Bloodworks
    Inventor: James Charles Zimring
  • Patent number: 10245312
    Abstract: New influenza donor strains for the production of reassortant influenza A viruses are provided.
    Type: Grant
    Filed: July 14, 2016
    Date of Patent: April 2, 2019
    Assignee: Seqirus UK Limited
    Inventors: Pirada Suphaphiphat, Peter Mason, Bjoern Keiner, Philip Dormitzer, Heidi Trusheim
  • Patent number: 10249035
    Abstract: A method includes capturing a signal of a detection substrate exposed to a sample containing a target substance; determining that the detection substrate is in a testable state; and generating an assessment of the presence of the target substance in the sample.
    Type: Grant
    Filed: September 29, 2016
    Date of Patent: April 2, 2019
    Assignee: Nima Labs, Inc.
    Inventors: Scott Sundvor, Ian Wallhead, Joseph Horrell, John Artiuch, Dane Weitmann, Stephen Wilson, Steven Portela, Jingqing Zhang, Francisco Dias Lourenco
  • Patent number: 10245356
    Abstract: The invention provides medical devices comprising improved non-uniform coatings for ultrasound detection, which provide optimal ultrasound images. Methods for preparing such devices are also provided.
    Type: Grant
    Filed: April 30, 2015
    Date of Patent: April 2, 2019
    Assignee: ENCAPSON B.V.
    Inventors: Lee Ayres, David Asrian, Johannes Antonius Opsteen, Dennis Manuel Vriezema
  • Patent number: 10246737
    Abstract: The present invention relates to methods for determining the presence and/or amount of multiple nucleic acids in a sample.
    Type: Grant
    Filed: February 23, 2015
    Date of Patent: April 2, 2019
    Assignee: ALERE TECHNOLOGIES GMBH
    Inventor: Thomas Ullrich
  • Patent number: 10241233
    Abstract: A method for characterizing an interaction between a first particle and a second particle is provided. The method includes the steps of: (i) providing an optical trap system including a photonics-based trap, a light source, and a camera; (ii) optically trapping, using the photonics-based trap, the first particle; (iii) obtaining a first measurement of a trap stiffness of the photonics-based trap; (iv) introducing the second particle to the optically trapped particle; (v) incubating the first and second particles under conditions suitable for an interaction between the first and second particles; (vi) obtaining a second measurement of the trap stiffness of the photonics-based trap after the incubation; and (vii) determining, using the first measurement of trap stiffness and the second measurement of trap stiffness, a property of the interaction between the first particle and the second particle.
    Type: Grant
    Filed: August 12, 2015
    Date of Patent: March 26, 2019
    Assignee: CORNELL UNIVERSITY
    Inventors: David Erickson, Pilgyu Kang
  • Patent number: 10238747
    Abstract: A vaccine for immunization against Type A influenza virus is provided having an immunologically effective amount of peptide constructs obtained by linking together two or more peptides based on or derived from different molecules, and methods for producing the same. The peptide constructs have the formula P1-x-P2 or P2-x-P1 where P1 is associated with Type A influenza highly conserved protein such as but not limited to M2e matrix protein, NP1 nucleoprotein, HA2 core 1, and HA2 core 2, where P2 is a peptide construct causing a Th1 directed immune response by a set or subset of T cells to which the peptide P1 is attached or that binds to a dendritic cell or T cell receptor causing said set or subset of dendritic cell or T cells to which the peptide P1 is attached to initiate and complete, an immune response, and x is a direct bond or divalent linker for covalently bonding P1 and P2.
    Type: Grant
    Filed: December 13, 2011
    Date of Patent: March 26, 2019
    Assignee: Cel-Sci, Corp
    Inventor: Daniel H. Zimmerman
  • Patent number: 10235622
    Abstract: A computing device identifies a pattern in a dataset. A first neural network model is executed using data points as input to input nodes of the first neural network model to generate first output node data. A second neural network model is executed using the first output node data as input to input nodes of the second neural network model to generate second output node data. The second output node data includes a plurality of output values for each x-value of the plurality of data points. For each x-value, an output value of the plurality of output values is associated with a single pattern type of a plurality of predefined pattern types. For each pattern type of the plurality of predefined pattern types, a start time and a stop time is identified when the output value for the associated pattern type exceeds a predefined pattern window threshold value.
    Type: Grant
    Filed: July 25, 2017
    Date of Patent: March 19, 2019
    Assignee: SAS INSTITUTE INC.
    Inventors: Stuart Andrew Hunt, Samuel Paul Leeman-Munk, Richard Welland Crowell
  • Patent number: 10234456
    Abstract: The present invention provides an easy-to-use and higher-sensitivity immunochromatography device for an RSV, the device using an antibody that binds specifically to F protein of the RSV in a determination region of a chromatography medium, a test kit by an immunochromatography method, and a method for detecting an RSV using these device and kit. The present invention relates to an immunochromatography device in which not only an antibody that binds specifically to F protein of an RSV but also an antibody that binds specifically to N protein of the RSV is solid-phased in a determination region of a chromatography medium. The present invention also relates to a test kit by an immunochromatography method, and a method for detecting an RSV using these device and kit.
    Type: Grant
    Filed: June 20, 2014
    Date of Patent: March 19, 2019
    Assignees: TANAKA KIKINZOKU KOGYO K.K., ALFERESA PHARMA CORPORATION
    Inventors: Mieko Kosaka, Hiromi Taguchi, Noboru Sakai, Daisuke Itoh, Hisahiko Iwamoto
  • Patent number: 10231998
    Abstract: A method of radiation-free hematopoietic stem cell (HSC) transplantation comprises administering to a mammalian subject one or two doses of 2 to 10 mg/kg body weight of a purine base analog, such as 6TG as a pre-conditioning step. The method further comprises engrafting into the subject hypoxanthine-guanine phosphoribosyltransferase (HPRT)-deficient donor HSCs within 48 to 72 hours of the pre-conditioning step; and administering to the subject about 1 to 5 mg/kg of the purine base analog every two to four days for two to eight weeks following the engrafting step. The method is performed in the absence of pre-conditioning via radiation. The subject is therefore not treated with myeloablative radiation in preparation for transplantation, and thus the subject is free of myeloablative radiation-induced toxicity.
    Type: Grant
    Filed: June 16, 2017
    Date of Patent: March 19, 2019
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Noriyuki Kasahara, Robert H. Schiestl, Katrin Hacke, Akos Szakmary, Gay M. Crooks
  • Patent number: 10227637
    Abstract: Provided are a PNA probe for detecting nucleotide polymorphism of a target gene, a melting curve analysis method for detecting the nucleotide polymorphism of the target gene using the same, a nucleotide polymorphism analysis method of a target gene including the melting curve analysis method, and a kit for detecting the nucleotide polymorphism of the target gene containing the PNA probe. It is characterized that the PNA probe according to the present invention contains negative charge molecules. The modified PNA probe according to the present invention contains the negative charge molecules to have a high recognition ability with respect to a target DNA and a high coupling ability to the target DNA and to be rapidly dissociated by heat, such that the nucleotide polymorphism analysis may be relatively easily performed even in a heterozygous sample showing two melting curve graphs, and two or more adjacent single nucleotide polymorphisms may be simultaneously analyzed.
    Type: Grant
    Filed: April 4, 2014
    Date of Patent: March 12, 2019
    Assignee: PANAGENE INC.
    Inventors: Goon Ho Joe, Sung-Kee Kim, Heekyung Park, Chwang Siek Park, Se Ryun Kim, Yongtae Kim, Su Nam Kim
  • Patent number: 10220383
    Abstract: A sampling and assay kit comprises a cuvette for holding a quantity of a buffer solution and a sample holder including a capillary tube for obtaining a sample. The sample holder includes a body portion which holds the capillary tube. The body portion when inserted in the cuvette, is effective to position the capillary tube within the cuvette at a predetermined position above the end of the cuvette.
    Type: Grant
    Filed: May 12, 2015
    Date of Patent: March 5, 2019
    Assignee: Orion Diagnostica OY
    Inventors: Jukka Laukkonen, Jaana Raussi, Sanna Eilola, Jaakko Rissanen
  • Patent number: 10214750
    Abstract: Systems and methods are disclosed herein for use in transducing, activating, and otherwise treating cells. Cells are introduced into an inner layer of a multi-layered stack that defines at least one flow chamber and a plurality of cell entrainment regions. Vertical flow through the stack entrains the cells in the cell entrainment regions along with genetic information introduction agents or other additives, before the cells are washed using a reverse vertical flow and are collected from the device.
    Type: Grant
    Filed: June 5, 2017
    Date of Patent: February 26, 2019
    Assignee: The Charles Stark Draper Laboratory, Inc.
    Inventors: Jeffrey T. Borenstein, Joseph L. Charest, Christopher M. DiBiasio, Dorit Berlin, Jenna Balestrini, Jose A. Santos, Vishal Tandon