Involving Virus Or Bacteriophage Patents (Class 435/5)
  • Patent number: 10808284
    Abstract: The present invention provides compositions, kits, and method for determining the levels of expression of human polyoma or papillomavirus species and RNA transcripts. These levels can be used for the prognosis of risk of developing virally-induced cancers. The ratio (R) between early and late transcripts is indicative of HPV infections associated with higher risk of developing genital neoplasia and cancer.
    Type: Grant
    Filed: July 7, 2015
    Date of Patent: October 20, 2020
    Assignees: INSTITUT PASTEUR, ECOLE NATIONALE VETERINAIRE D'ALFORT, PATHQUEST, L'ASSISTANCE PUBLIQUE—HOPITAUX DE PARIS
    Inventors: Marc Eloit, Nicolas Torno, Jean Deregnaucourt, Marion Mouton, Isabelle Heard, Philippe Perot
  • Patent number: 10808287
    Abstract: Diagnostic devices test markers for viral infection and markers for bacterial infection to effectively assist in the rapid differentiation of viral and bacterial infections, to differentiate between colonization and active infection, and to better diagnose microbiologically unconfirmed patients. In other embodiments, detecting a presence of MxA in combination with either the bacterial biomarker C-reactive protein or the bacterial biomarker procalcitonin increases the specificity of the bacterial biomarker with a concurrent improvement in sensitivity.
    Type: Grant
    Filed: February 2, 2016
    Date of Patent: October 20, 2020
    Assignee: Rapid Pathogen Screening, Inc.
    Inventors: Robert P. Sambursky, Robert W. VanDine, Uma Mahesh Babu
  • Patent number: 10809228
    Abstract: Volume susceptibilities (?s) of dispersoid particles (s) dispersed in a dispersion medium (m) are first obtained by magnetophoresis. Affinity of the dispersoid particles (s) for the dispersion medium (m) is then analyzed using the volume susceptibilities (?s) of the respective dispersoid particles (s) and a volume susceptibility (?m) of the dispersion medium (m).
    Type: Grant
    Filed: June 24, 2016
    Date of Patent: October 20, 2020
    Assignee: KAWANO Lab. Inc.
    Inventor: Makoto Kawano
  • Patent number: 10792101
    Abstract: A method and system of ablating melanin are provided. Stepwise multi-photon fluorescence is induced in melanin within a region of tissue. The fluorescence is detected, and at least a portion of the melanin from which the fluorescence is detected is ablated. The system and method can use a continuous wave laser in the near infrared range for the inducement and ablation of melanin, providing high resolution at low cost.
    Type: Grant
    Filed: December 17, 2019
    Date of Patent: October 6, 2020
    Inventor: Zhenhua Lai
  • Patent number: 10793901
    Abstract: The present invention provides for stable nucleotide reagents used for nucleic acid amplification by PCR and RT-PCR (Reverse Transcriptase-PCR) that comprises modified nucleoside polyphosphates. The present invention also provides for methods for using the modified nucleoside polyphosphates for detecting the presence or absence of a target nucleic acid sequence in a sample in an amplification reaction.
    Type: Grant
    Filed: December 18, 2017
    Date of Patent: October 6, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventor: Amar Gupta
  • Patent number: 10794908
    Abstract: The present invention discloses functionalized nanomembranes, a method for preparation and their use. The functionalized nanomembrane comprises a) a first layer comprising a nanomaterial, b) a second layer comprising a biorepulsive material, the second layer being attached to at least one side of the first layer, and c) affinity groups, attached to the second layer.
    Type: Grant
    Filed: January 29, 2016
    Date of Patent: October 6, 2020
    Assignees: Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Quantifoil Micro Tools GmbH
    Inventors: Andreas Terfort, Daniel Rhinow, Andrey Turchanin
  • Patent number: 10787487
    Abstract: Presented herein are cyclic peptides that specifically bind to a cell surface molecule, thereby allowing cell/tissue-specific targeting. The cyclic peptides can be attached to an agent, for example, a polypeptide such as an antibody, e.g., a cell-penetrating antibody. Cyclic peptide-containing cell/tissue-specific cell-penetrating antibodies described herein are capable of targeted delivery in a cell type-specific or tissue-specific (i.e., cell/tissue-specific) manner. The cell/tissue-specific cell-penetrating antibodies described herein can be used as an effective anticancer agent for cancer that overexpresses a cell membrane protein that specifically binds to the fused cyclic peptides.
    Type: Grant
    Filed: June 20, 2019
    Date of Patent: September 29, 2020
    Assignee: ORUM THERAPEUTICS INC.
    Inventors: Yong Sung Kim, Ji Sun Kim, Jae Yeong Park, Seong Wook Park, Sei Yong Jun, Dong-Ki Choi
  • Patent number: 10781485
    Abstract: Disclosed are methods for determining at least one sequence of interest of a fetus of a pregnant mother. In various embodiments, the method can determine one or more sequences of interest in a test sample that comprises a mixture of fetal cellular DNA and mother-and-fetus cfDNA. In some embodiments, methods are provided for determining whether the fetus has a genetic disease. In some embodiments, methods are provided for determining whether the fetus is homozygous in a disease causing allele when the mother is heterozygous of the same allele. In some embodiments, methods are provided for determining whether the fetus has a copy number variation (CNV) or a non-CNV genetic sequence anomaly.
    Type: Grant
    Filed: April 20, 2018
    Date of Patent: September 22, 2020
    Assignee: Illumina, Inc.
    Inventors: AmirAli Hajhossein Talasaz, Gordon M. Cann
  • Patent number: 10775371
    Abstract: The present disclosure provides methods for the detection of multiple analytes using a single solid phase. The present disclosure also relates to the preparation of solid phases that include receptacles having affixed thereto antibodies directed to at least two different analytes. The methods of the present disclosure can be used, for example, for the quantitative detection of analytes in a sample and the measurement thereof.
    Type: Grant
    Filed: March 2, 2018
    Date of Patent: September 15, 2020
    Assignee: ORTHO-CLINICAL DIAGNOSTICS, INC.
    Inventors: Godwin Ogbonna, Shari Jackson, Timothy Mangan, Jody Parsells
  • Patent number: 10769784
    Abstract: An image analyzing method is provided and includes: extracting a first feature vector according to global information of a digital image; dividing the digital image into multiple regions, and inputting each region into a convolutional neural network to obtain a second feature vector; merging the first feature vector with the second feature vectors to obtain a third feature vector; and performing an image analyzing process according to the third feature vector.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: September 8, 2020
    Assignee: METAL INDUSTRIES RESEARCH & DEVELOPMENT CENTRE
    Inventors: Sheng-Hong Yang, Jian-Jia Zeng, Bo-Wei Pan
  • Patent number: 10768086
    Abstract: In determining the size of particles in a flowing sample, initially scattered light is detected on a detector, in the form of images, standardization of the measured images takes place and determination of an offset and of an angle of rotation at least of a partial region of two temporally successive images relative to each other is effected. Two images congruent are subdivided into many partial areas with respectively a specific number of pixels and averaging of the brightness values of each individual partial area is implemented. Subsequently, the average brightness value of a partial area of the first image is correlated with the average brightness value of the partial area made congruent with this partial area in the second image (cross-correlation between the images). For each pair of congruent partial areas of the images, a correlation value is thereby produced.
    Type: Grant
    Filed: July 3, 2017
    Date of Patent: September 8, 2020
    Assignee: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
    Inventors: Peter Höbel, Michael Maskos
  • Patent number: 10767179
    Abstract: Certain embodiments are directed to methods of identifying neuroblastoma differentiation-inducing compounds or agent and their use in treating neuroblastoma.
    Type: Grant
    Filed: October 1, 2018
    Date of Patent: September 8, 2020
    Assignee: THE BOARD OF REGENTS OF THE UNIVERSITY OF TEXAS SYSTEM
    Inventor: Liqin Du
  • Patent number: 10765744
    Abstract: Embodiments of the present disclosure provide for magnetic particle conjugates, methods of making the magnetic particle conjugates, methods of using magnetic particle conjugates, micelles (also referred to as a “magnetic composite nanocarrier” (MC-NC)), methods of making micelles, methods of using micelles, and the like.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: September 8, 2020
    Assignee: University of Florida Research Foundation, Inc.
    Inventors: Carlos Rinaldi, Sun Hao, Brent S. Sumerlin
  • Patent number: 10760118
    Abstract: The invention relates to an in vitro method for selectively amplifying a target DNA sequence from a nucleic acid sample, which method comprises running a PCR amplification of a nucleic acid sample suspected of comprising at least one target DNA sequence that differs from a reference DNA sequence at at least one predetermined target mutation site; wherein said method employs a blocking oligonucleotide complementary to a portion of the reference DNA sequence comprising the target mutation site, with the exception of a least one mismatch outside the target mutation site.
    Type: Grant
    Filed: April 29, 2014
    Date of Patent: September 1, 2020
    Assignee: QIAGEN GMBH
    Inventor: Olivier Biglia
  • Patent number: 10753932
    Abstract: An immunoassay test apparatus, including: a label signal sensor that detects label signals emitted by at least one label conjugated to an antibody or antigen of an immunoassay test device such as a lateral flow test strip or microfluidic cartridge, and outputs sensor data representing the detected label signals; a label imaging component that processes the sensor data to generate label data representing spatial locations of the detected label signals; a test result image generator that processes the label data to generate test result image data representing a visual image of the spatial arrangement of the detected label signals; and a display component that displays the test result image to a user of the immunoassay test apparatus to enable the user to evaluate the result of the immunoassay test.
    Type: Grant
    Filed: September 4, 2015
    Date of Patent: August 25, 2020
    Assignee: Axxin Pty Ltd
    Inventor: William R. Hopper
  • Patent number: 10752963
    Abstract: A micro-fluidic chip and its modification method and application in detection of food bacteria quality, includes the following steps: changing a functional group —CH3 on the internal surface of micro-fluidic channel of micro-fluidic chip to a functional group —OH through modification; Supplying amino silane reagent to the micro-fluidic channel; supplying dendritic polymer as modified by —COOH to internal surface of the micro-fluidic channel after drying; grafting primer of aminated aptamer RCA on terminus 5? and hybrid from its padlock probe on dendrimer on internal surface of the micro-fluidic channel; wherein, the padlock probe is the complementary sequence of the aptamer of the target pathogenic bacteria to be tested; after that, supplying RCA reaction reagent to the micro-fluidic channel to make aptamer RCA generate long-chain aptamer in series. The present invention adopts two RCA reactions of varied functions in combination, and uses dendritic polymer to modify internal surface of the chip.
    Type: Grant
    Filed: July 24, 2018
    Date of Patent: August 25, 2020
    Assignee: GREENTOWN AGRICULTURAL TESTING TECHNOLOGY CO., LTD.
    Inventors: Xingkai Hao, Yuqian Jiang, Xudong Cao, Chuanpi Wang, Min Zhou, Meina Sun, Zhen Wang
  • Patent number: 10746692
    Abstract: Systems and methods for detection of biological agents are generally described. Target biological agents may be detected by use of a sensor, which in some situations is a nanowire. An external electric field is applied in some embodiments to induce an electric dipole. The induced electric dipole is detected, allowing detection of the biological agent.
    Type: Grant
    Filed: July 22, 2015
    Date of Patent: August 18, 2020
    Assignee: FemtoDx
    Inventors: Pritiraj Mohanty, Shyamsunder Erramilli
  • Patent number: 10745445
    Abstract: Disclosed are agents (e.g., peptides, polypeptides, proteins, small molecules, antibodies, and antibody fragments that target senescent cells) and methods of their use for imaging senescent cells in vivo and for treating or preventing cancer, age-related disease, tobacco-related disease, or other diseases and disorders related to or caused by cellular senescence in a mammal. The methods include administering one or more of the agents of the invention to a mammal, e.g., a human. The agents, which specifically bind to senescent cells, can be labeled with a radioactive label or a therapeutic label, e.g., a cytotoxic agent.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: August 18, 2020
    Assignee: Unity Biotechnology, Inc.
    Inventor: Shayne Squires
  • Patent number: 10745767
    Abstract: Compositions, methods and kits for detecting viral nucleic acids. Targets that can be detected in accordance with the invention include HBV and/or HIV-1 and/or HCV nucleic acids. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers that facilitate detection of very low levels of HBV nucleic acids.
    Type: Grant
    Filed: January 25, 2018
    Date of Patent: August 18, 2020
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Jeffrey M. Linnen, Daniel P. Kolk, Janel M. Dockter, Damon K. Getman, Tadashi Yoshimura, Martha K. Ho-Sing-Loy, Reinhold B. Pollner, Leslie A. Stringfellow
  • Patent number: 10739343
    Abstract: The present invention concerns a device for measuring the concentration of analytes in liquid samples such as bodily samples. The device comprises an application zone, to which a sample can be applied, and which contains a specific molecule capable of specifically binding the analyte of interest, said specific molecule being conjugated to a reporter which can give rise to variations in impedance. The resulting complex migrates by capillarity and enters a detection zone, on which another molecule capable of specifically binding the analyte of interest is immobilised. The concentration of reporter molecules in the detection zone is proportional with the concentration of analyte in the sample, and variations in concentration of reporter molecules yield a measurable change in electrical properties such as a change in impedance and/or capacitance which can be correlated to the concentration of analyte.
    Type: Grant
    Filed: August 16, 2018
    Date of Patent: August 11, 2020
    Inventors: Jesper Fleischer, Michael Hasenkam, Niels E. Magnusson, Hans Nygaard
  • Patent number: 10738342
    Abstract: Several microfluidic chips are used to significantly accelerate the time to identify and quantify microbes in a biological sample and test them for antibiotic resistance, particularly for urinary tract infections. A first microfluidic chip uses antibody or similar probes to identify and quantify any microbes present. The same or a similar chip uses antibody or similar probes to identify microbes with DNA or RNA known to indicate antibiotic resistance. Another microfluidic chip tests for antibiotic susceptibility of any microbes by growing them in very small wells in the presence of antibiotics, reducing the time required for such testing by as much as 95%. Another microfluidic chip runs traditional urinalysis or similar tests.
    Type: Grant
    Filed: August 26, 2019
    Date of Patent: August 11, 2020
    Assignee: Urinary Technologies, Inc.
    Inventors: Michael D. Finch, Kee Onn Fong, Saurabh Kotian, Cyrus B. Munshi, Advitiya Mahajan, Beth A. Lindborg
  • Patent number: 10732110
    Abstract: A quantitative method for diagnosing an autoimmune disease or an infectious disease comprising performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex, wherein the capture reagent is a biotinylated autoantigen or infectious disease antigen; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to form an immune complex; washing the immune complex to remove any unbound sample; incubating the immune complex with a conjugate to create an immune-conjugate complex; washing the immune-conjugate complex to remove any unbound conjugate; introducing a substrate capable of generating a quantifiable response; and calibrating the response generated from introducing the substrate.
    Type: Grant
    Filed: April 7, 2017
    Date of Patent: August 4, 2020
    Assignee: HYCOR Biomedical, LLC
    Inventors: Mark David Van Cleve, Elaine Grace Taine, Douglas John Canfield, Stephanie TuVi Ortega, Taylor Addison Reid
  • Patent number: 10732166
    Abstract: A method for in-line measurement of the quality of a microarray are disclosed and the method includes the following steps. A solid substrate is provided, and the solid substrate includes a plurality of areas in an array. At least one biomarker is in-situ synthesized on at least one of the plurality of areas by a plurality of synthesis steps. After performing at least one of the plurality of synthesis step, a check step is immediately performed on a semi-product of the at least one biomarker by an atomic force microscope to obtain an in-line measurement result. The quality of the semi-product of the at least one biomarker is determined based on the in-line measurement result.
    Type: Grant
    Filed: February 14, 2018
    Date of Patent: August 4, 2020
    Assignee: Centrillion Technologies Taiwan Co. LTD.
    Inventors: Tzu-Kun Ku, Yao-Kuang Chung, Yu-Chen Wang, Po-Yen Liu
  • Patent number: 10731156
    Abstract: Provided are oligonucleotides capable of binding to and modulating the splicing of the pre-mRNA of the CFTR gene, compositions including said oligonucleotides, kits including the compositions, and uses thereof. In particular, the subject matter provides compositions of oligonucleotides useful in methods for suppressing exon skipping optionally in combination with additional CFTR therapeutics.
    Type: Grant
    Filed: January 12, 2018
    Date of Patent: August 4, 2020
    Assignee: Yissum Research Development Company of the Hebrew University of Jerusalem Ltd.
    Inventors: Batsheva Kerem, Michal Tur Sinai, Loren Price, Stephen Donald Wilton, Sue Fletcher
  • Patent number: 10729082
    Abstract: The present application relates to papaya plants having increased resistance to the papaya ringspot virus as compared to a wild type plant due to a mutation in the eukaryotic translation initiation factor 4e and/or eukaryotic translation initiation factor iso4e gene leading to non-functional eukaryotic translation initiation factor 4e and/or eukaryotic translation initiation factor iso4e proteins. Methods of producing such papaya plants having increased resistance to the papaya ringspot virus are described.
    Type: Grant
    Filed: October 16, 2017
    Date of Patent: August 4, 2020
    Assignees: BENCHBIO PVT. LTD., NAMDHARI PVT. LTD, HORTINGENETICS RESEARCH (S.E. ASIA) LTD.
    Inventors: Manash Chatterjee, Anish Kumar Pullanchyottu Kizhakkeveettil, Anand Narasimhan, Krishna Katta Ashwathaiah, Darush Struss, Conrado H. Balatero
  • Patent number: 10731203
    Abstract: The present invention relates to the detection of a target nucleic acid sequence by a PCE-SH (PTO Cleavage and Extension-Dependent Signaling Oligonucleotide Hybridization) assay. The present invention does not use probes to be hybridized with target nucleic acid sequences for providing target signals. Interestingly, the present invention uses probes (signaling oligonucleotides) to be hybridized with the extended strand formed in a target-dependent manner in which the extended strand is synthesized using the CTO artificially selected as templates.
    Type: Grant
    Filed: December 27, 2017
    Date of Patent: August 4, 2020
    Assignee: SEEGENE, INC.
    Inventors: Jong Yoon Chun, Young Jo Lee
  • Patent number: 10731227
    Abstract: Provided herein include compositions comprising a primer having the nucleic acid sequence of ACeIN-F3_c, a primer having the nucleic acid sequence of ACeIN-B3_a, a primer having the nucleic acid sequence of ACeIN-B3_b, a primer having the nucleic acid sequence of ACeIN-FIP_e, a primer having the nucleic acid sequence of ACeIN-FIP_f, a primer having the nucleic acid sequence of ACeIN-BIP (or ACeIN-BIP-song), a primer having the nucleic acid sequence of ACeIN-LF; and a primer having the nucleic acid sequence of ACeIN-LB. Also provided are methods of detecting human immunodeficiency virus (HIV) nucleic acids in a sample comprising performing reverse transcription-based loop mediated isothermal amplification (RT-LAMP) on a sample using the previously disclosed compositions.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: August 4, 2020
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Changchun Liu, Scott Sherrill-Mix, Haim H. Bau, Frederic D. Bushman, Karen E. Ocwieja, Jinzhao Song
  • Patent number: 10732180
    Abstract: A universal array for multiplexed quantification of variable hemagglutinin such as subcomponents of multivalent annual influenza vaccines that is robust to variations in proteins such as mutations and is capable of quantifying degradation of proteins. Universal capture array (100) comprises one or more substrates (102) and a low-density microarray (104) of sub-arrays (108) comprising spots (106a-c). The microarray (104) is contacted with one or more targets (202) at one or more unknown concentrations, and bound complexes (203) are formed and subsequently quantified with a suitable method. Quantified signals are compared to calibration curves to obtain one or more unknown concentrations and/or quantify degradation of the one or more targets (202). Other embodiments are described and shown.
    Type: Grant
    Filed: June 4, 2014
    Date of Patent: August 4, 2020
    Assignee: INDEVR, INC.
    Inventors: Kathy Rowlen, Laura R. Kuck
  • Patent number: 10734072
    Abstract: Disclosed is a molecularly imprinted polymer for storing a defined value of a numerical code, more particularly a binary code, in the molecular imprints of said polymer, and a method for the production of said polymer. The molecular imprinting process uses suitable templates comprising a defined sequence of at least two different structural units, each having a different chemical functionality.
    Type: Grant
    Filed: April 27, 2017
    Date of Patent: August 4, 2020
    Assignee: JOHANNES KEPLER UNIVERSITÄAT LINZ
    Inventors: Klaus Mosbach, Oliver Brüggemann, Jacqueline Wolfschlucker
  • Patent number: 10724111
    Abstract: Provided herein are methods for determining the presence or absence of an enteroviruses and parechoviruses in a biological sample. The methods involve identifying the presence or absence of a target nucleic acids from the viruses using direct amplification from a biological sample without a step of extraction of the nucleic acids, but retaining substantially the same specificity and sensitivity of methods assaying extracted nucleic acids. Also provided are methods of diagnosis using the methods provided and compositions and kits for the practice of the methods.
    Type: Grant
    Filed: June 10, 2019
    Date of Patent: July 28, 2020
    Assignee: Quest Diagnostics Investments LLC
    Inventors: Peter Lee, Lakshmi Nair, Albert Castro, Maria Vestal, Michelle Tabb
  • Patent number: 10722534
    Abstract: A magnetic nanoaggregate-embedded bead (NAEB), a manufacturing method thereof and a bioparticle detection method using the same are disclosed. The magnetic NAEB has a protective nanoshell, noble metal nanoparticles, Raman reporter molecules and at least one magnetic nanoparticle. The protective nanoshell covers the noble metal nanoparticles, the Raman reporter molecules and the at least one magnetic nanoparticle. The noble metal nanoparticles, the Raman reporter molecules and the at least one magnetic nanoparticle form a magnetic nanoaggregate. Preferably, a chemical modification is performed on the magnetic NAEB, such that at least one targeting molecule is formed on an outer wall of the protective nanoshell, wherein a type of the targeting molecule is corresponding to a type of the bioparticle to be detected.
    Type: Grant
    Filed: July 12, 2017
    Date of Patent: July 28, 2020
    Assignee: National Chung Cheng University
    Inventors: Lai-Kwan Chau, Shih-Ying Yen
  • Patent number: 10725030
    Abstract: The present invention provides a method of assaying a sample solution for the presence of a first analyte comprising: (a) providing a sensor surface having a ligand immobilized thereto; (b) flowing the sample solution over the sensor surface; and (c) detecting the presence or absence of binding of the analyte to the ligand on the sensor surface; wherein the contact time between the sample solution and the immobilized ligand is less than 15 seconds.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: July 28, 2020
    Assignee: GE HEALTHCARE BIO-SCIENCES AB
    Inventor: Olof Karlsson
  • Patent number: 10725033
    Abstract: Assays used in conjunction with a thermal contrast reader are disclosed. In the assay, the test strip includes materials that can develop a thermal response if a target analyte is present in a sample. Linear flow assays include nanoparticles with high affinity binding to the analyte. Binding of the nanoparticles with an analyte in the sample is detected using thermal contrast. Analytes over a broad range of concentrations are detected in the linear flow assays. Methods of detecting target analytes and kits comprising lateral flow assays and thermal contrast reader are also disclosed.
    Type: Grant
    Filed: November 2, 2017
    Date of Patent: July 28, 2020
    Assignees: REGENTS OF THE UNIVERSITY OF MINNESOTA, THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
    Inventors: John C. Bischof, Zhenpeng Qin, Warren Chan, Taner Akkin, Li Zhan
  • Patent number: 10724031
    Abstract: The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
    Type: Grant
    Filed: September 27, 2019
    Date of Patent: July 28, 2020
    Assignee: BIONEER CORPORATION
    Inventors: Han Oh Park, Jae Ha Kim, Jong Gwang Park
  • Patent number: 10718004
    Abstract: Techniques, systems, and devices are described for implementing a droplet array for single-cell analysis. A method of conducting single-cell analysis comprises generating a plurality of droplets, wherein each of the plurality of droplets contains a core material surrounded by a protective shell; loading the plurality of droplets, via a carrying fluid, onto an array including a plurality of trap structures, wherein the plurality of droplets are held by the plurality of trap structures; selecting a target droplet, held by a trap structure, from the plurality of droplets; and releasing the target droplet from the trap structure.
    Type: Grant
    Filed: December 20, 2016
    Date of Patent: July 21, 2020
    Assignee: Lawrence Livermore National Security, LLC
    Inventors: Melinda Simon, N. Reginald Beer
  • Patent number: 10718772
    Abstract: A method for detection of active form of analytes in a sample and/or for determination of ability of tested substances to bind to the active site of these analytes has the following steps: a) analyte or group of analytes from the sample is immobilized on the surface of a solid carrier; b) analyte or group of analytes is incubated with a detection probe; c) then the solid carrier is washed to remove unbound detection probe; and subsequently, the amount of bound detection probe is determined.
    Type: Grant
    Filed: August 4, 2015
    Date of Patent: July 21, 2020
    Assignee: USTAV ORGANICKE CHEMIE A BIOCHEMIE AKADEMIE VED CR, V.V.I.
    Inventors: Vaclav Navratil, Pavel Sacha, Jiri Schimer, Jan Konvalinka, Pavel Majer
  • Patent number: 10718713
    Abstract: In a standard process for determining an unknown sample, fluorescent substances are determined from respective fluorescence characteristics and model coefficients are calculated from spectrum ranges of the fluorescence characteristics of the determined fluorescent substances. An unknown sample is measured after reading of the model coefficients, whereby a target value of the unknown sample is obtained.
    Type: Grant
    Filed: September 6, 2017
    Date of Patent: July 21, 2020
    Assignee: Hitachi High-Tech Science Corporation
    Inventors: Jun Horigome, Koichi Nakamura, Yoichi Sato, Yusuke Hosen, Michinari Kozuma
  • Patent number: 10704101
    Abstract: A method and kit for detecting a genetic variant associated with a disease or disorder, including incompatibility with a pharmaceutical. The method and kit using a first nano-particle coupled to at least one morpholino nucleic acid probe comprising a target complimentary region base sequence that is a perfect match to a genetic variant sequence.
    Type: Grant
    Filed: July 11, 2018
    Date of Patent: July 7, 2020
    Assignee: Agency for Science, Technology and Research
    Inventors: Yanbing Zu, Jackie Y. Ying
  • Patent number: 10695368
    Abstract: A method of preventing protein glycation using a supplement may comprise: administering a supplement comprising lysine and zinc; monitoring a concentration of glucose before and after the supplement is administered in a bio-sample; and determining a change in a dose of the supplement based on the concentration of glucose found in the bio-sample.
    Type: Grant
    Filed: May 11, 2018
    Date of Patent: June 30, 2020
    Assignee: Lysulin, Inc.
    Inventor: John Burd
  • Patent number: 10697964
    Abstract: This invention describes a one-step technique for the simultaneous label-free detection and concentration of blast cells from a blood sample. Enrichment of blast cells is achieved using a closed loop microfluidics system, allowing continuous removal of waste and non-target cells to generate concentrated samples of high purity without the need for specific targeting of proteins by antibodies. The technique is highly effective for samples which cannot be purified in a single run. The application of detecting rare blast cells for monitoring minimal residual disease in leukemia patients is demonstrated. The sensitivity of the invention promotes the detection of blast cells in blood samples of early-stage patients.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: June 30, 2020
    Assignee: Massachusetts Institute of Technology
    Inventors: Bee Luan Khoo, Jongyoon Han, Kyungyong Choi, Hyunryul Ryu
  • Patent number: 10697028
    Abstract: Disclosed herein are methods of detecting HIV-1 nucleic acids in a sample (such as from a sample containing or suspected to contain HIV-1 nucleic acid). In some examples, the methods include loop-mediated isothermal amplification (LAMP) or reverse transcription-LAMP (RT-LAMP). In some examples, the methods include contacting a sample with one or more sets of LAMP primers specific for HIV-1 (such as LAMP primers specific for an HIV-1 integrase nucleic acid or LAMP primers specific for an HIV-1 reverse transcriptase nucleic acid) under conditions sufficient to produce an amplification product and detecting the amplification product. Sets of LAMP primers for detection of HIV-1 integrase nucleic acids (such as SEQ ID NOs: 8-14 or 8-27) and HIV-1 reverse transcriptase nucleic acids (such as SEQ ID NOs: 1-7) are provided herein.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: June 30, 2020
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Kelly A. Curtis, Sherry M. Owen, Philip Niedzwiedz, Donna L. Rudolph
  • Patent number: 10696717
    Abstract: The invention relates to novel peptides, polypeptides or proteins which specifically bind to cells of the brain and/or the spinal cord. The peptides, polypeptides or proteins can be part of a viral capsid, and they can be used for guiding a recombinant viral vector selectively to the brain and/or spinal cord after systemic administration to a subject, where it provides for a tissue-specific expression of one or more transgenes. The invention therefore also relates to a recombinant viral vector, preferably an AAV vector, comprising a capsid containing at least one of the peptides, polypeptides or proteins of the invention and at least one transgene which is packaged within the capsid. The viral vector is particularly suitable for the therapeutic treatment of a disease or functional disorder of the brain and/or the spinal cord. The invention further relates to cells and pharmaceutical compositions comprising the viral vector of the invention.
    Type: Grant
    Filed: January 31, 2019
    Date of Patent: June 30, 2020
    Assignee: Boehringer Ingelheim International GmbH
    Inventors: Jakob Körbelin, Stefan Michelfelder, Martin Trepel
  • Patent number: 10690669
    Abstract: Methods, compositions, devices, and kits are described herein that are useful for detecting BoHV-1 infection in animals and/or for distinguishing animals that may benefit from administration of BoHV-1 tmv vaccine.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: June 23, 2020
    Assignee: BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY AND AGRICULTURAL AND MECHANICAL COLLEGE
    Inventor: Shafiqul I. Chowdhury
  • Patent number: 10689718
    Abstract: A method of simultaneously amplifying genotypes 1, 2, 3 and/or 4 of HEV is disclosed comprising amplifying the genotypes 1, 2, 3 and/or 4 of HEV with one single none-degenerate forward primer partially overlapping the 5?UTR region of HEV and at least one reverse primer. Also disclosed are related methods comprising a probe, and kits for the detection of genotypes 1, 2, 3 and/or 4 of HEV.
    Type: Grant
    Filed: June 2, 2017
    Date of Patent: June 23, 2020
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Hermann Leying, Thomas W. Myers, Nicolas Newton, Eberhard Russmann, Joseph San Filippo, Nancy Schoenbrunner, Heike Wilts, Xiaoning Wu, Karen K. Y. Young, Dirk Zimmermann
  • Patent number: 10690667
    Abstract: A single-use multiplex, or assay, screening test for the detection of one or more of a plurality of unrelated febrile illnesses is provided. The febrile illnesses for which the test is designed are unrelated, in that the illnesses may be caused, by way of example, by infection from viruses, bacterium and/or parasites; by infection from viruses, bacterium, parasites or other contagions that are animal borne; by infection from viruses, bacterium, parasites, or other contagions that can be aerosolized for transmission; by infection from viruses, bacterium, parasites or other contagions that are transmitted from direct contact; by infection from viruses, bacterium, parasites or other contagions that are generally transmitted in the tropics and/or subtropics; and/or by infection from a virus, bacteria, parasite sharing one or more related feature and which causes a febrile illness.
    Type: Grant
    Filed: September 7, 2018
    Date of Patent: June 23, 2020
    Assignee: Chembio Diagnostic Systems, Inc.
    Inventors: Javanbakhsh Esfandiari, Angelo H. Gunasekera
  • Patent number: 10684278
    Abstract: Among other things, two or more different antibodies are caused to bind to one or more units of a chemical component in a sample. Each of the antibodies is attached to one or more beads (e.g., microbeads). The sample is situated on a surface of an image sensor. At the image sensor, light is received originating at a light source that is other than the beads. The received light includes light reflected by, refracted by, or transmitted through the beads. The image sensor captures one or more images of the sample including the beads. At least one of the images of the sample is processed to separately enumerate individual beads and complexes of two or more of the beads attached to the two or more antibodies that are bound to a unit of the chemical component. The results of the processing are used to identify a presence or a level of the chemical component in the sample.
    Type: Grant
    Filed: March 28, 2019
    Date of Patent: June 16, 2020
    Assignee: Alentic Microscience Inc.
    Inventor: Alan Marc Fine
  • Patent number: 10683357
    Abstract: Provided herein are antibodies, or antigen binding portions thereof, that bind to OX40. Also provided are uses of these proteins in therapeutic applications, such as in the treatment of cancer. Further provided are cells that produce the antibodies, polynucleotides encoding the heavy and/or light chain variable region of the antibodies, and vectors comprising the polynucleotides encoding the heavy and/or light chain variable region of the antibodies.
    Type: Grant
    Filed: March 30, 2017
    Date of Patent: June 16, 2020
    Assignee: Bristol-Myers Squibb Company
    Inventors: Zhehong Cai, Indrani Chakraborty, Marie-Michelle Navarro Garcia, Thomas D. Kempe, Alan J. Korman, Alexander T. Kozhich, Hadia Lemar, Mark Maurer, Christina Maria Milburn, Michael Quigley, Xiang Shao, Mohan Srinivasan, Kent Thudium, Susan Chien-Szu Wong, Jochem Gokemeijer, Xi-Tao Wang, Han Chang, Patrick Guirnalda
  • Patent number: 10674724
    Abstract: A method of enhancing plant immunity is provided. The method comprises the step of administering to a plant a small molecule that binds to NPR1, or a functionally equivalent homolog thereof, that disrupts the interaction between N-terminal BTB/POZ domain and the C-terminal transactivation domain of NPR1. A method of screening for small molecule compounds that enhance plant immunity is also provided.
    Type: Grant
    Filed: September 8, 2016
    Date of Patent: June 9, 2020
    Inventor: Charles Despres
  • Patent number: 10677805
    Abstract: The invention relates to color-changing eye drops for early screening Alzheimer's disease and an application thereof. The color-changing eye drop for early screening Alzheimer's disease comprises 1 pg/ml to 10 ng/ml of A?42 aptamer-gold nanoparticles (AuNPs) and a pharmaceutically acceptable carrier or vehicle thereof, which can be used to mix with a tear sample of a test subject to analyze a color change of the color-changing eye drop for determining the concentration proportion of A?40:A?42 contained in the tear sample of the test subject.
    Type: Grant
    Filed: June 14, 2019
    Date of Patent: June 9, 2020
    Assignee: NATIONAL CHIAO TUNG UNIVERSITY
    Inventors: Jung-Chih Chen, I-Chiu Li, Kun-Che Li, Ching-Cheng Chuang, Mei-Lan Ko, Han-Chien Chuang, Ming-Hung Chien, Yu-Rong Wang, Hung-Ru Wang
  • Patent number: 10677779
    Abstract: Disclosed is a platelet-testing chip. The platelet-testing chip includes a plurality of sample chambers storing blood samples, stirrers provided in the sample chambers to apply shearing force to the blood samples, a plurality of waste sample chambers provided so as to correspond to the plurality of the sample chambers, microchannels, through which the sample chambers and the waste sample chambers corresponding to each other are independently connected to thus form paths through which the blood samples flow from the sample chambers to the corresponding waste sample chambers, and microbeads which are received in one or more of the plurality of the sample chambers and which are coated with a reagent for activating platelets on an outer surface thereof. When the blood samples are transferred from the sample chambers through the microchannels, the microbeads are transferred together with the blood samples.
    Type: Grant
    Filed: February 23, 2016
    Date of Patent: June 9, 2020
    Assignee: Korea University Research and Business Foundation
    Inventor: Se-Hyun Shin