At 11-position Patents (Class 435/59)
  • Patent number: 6828120
    Abstract: The present invention is a novel process for the transformation of 17&agr;-hydroxy-6&agr;-methylpregn-4-ene-3,20-dione 17-acetate (I) to 11&bgr;,17&agr;,21-trihydroxy-6&agr;-methylpregna-1,4-diene-3,20-dione 21-acetate (VI)
    Type: Grant
    Filed: June 14, 2002
    Date of Patent: December 7, 2004
    Assignee: Pharmacia and Upjohn Company
    Inventors: Bruce Allen Pearlman, Michael J. White, Ivan G. Gilbert
  • Patent number: 6632633
    Abstract: An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins of catalyzing an oxidation step in the biological pathway, for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17&agr;-hydroxyprogesterone; the conversion of 17&agr;-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in that host.
    Type: Grant
    Filed: June 17, 1998
    Date of Patent: October 14, 2003
    Assignee: Aventis Pharma, S.A.
    Inventors: Herman Slijkhuis, Eric Bastiaan Smaal, Gerardus Cornelis Maria Selten
  • Publication number: 20030108982
    Abstract: An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting a of:
    Type: Application
    Filed: February 26, 2002
    Publication date: June 12, 2003
    Applicant: Aventis Pharma S.A.
    Inventors: Herman Slijkhuis, Eric Bastiaan Smaal, Gerardus Cornelis Maria Selten
  • Patent number: 5723319
    Abstract: Novel cell lines and methods are provided for inducibly expressing the Hepatitis B virus (HBV) genome within cultured cells. Cells are stably transformed with an HBV genome under control of an operator/promoter target sequence which can activate expression of the HBV genome when bound by a cognate transactivator protein. The cell lines are also stably transformed with a gene that encodes and expresses a suitable transactivator protein. Cells may be maintained under non-inducing conditions whereby the transactivator protein is unable to interact with the operator/promoter target sequence, so the HBV genome is not expressed. Under appropriate inducing conditions, the transactivator protein is enabled to interact with the operator/promoter target sequence, thereby activating expression of the HBV genome. The inducible system provides an effective and rapid cell-based assay to screen compounds for anti-hepadnaviral activity.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: March 3, 1998
    Assignees: Avid Therapeutics, Inc., Fox Chase Cancer Center
    Inventors: Robert W. King, Christopher S. Barker, Christoph Seeger
  • Patent number: 4588683
    Abstract: Disclosed herein is a method for preparing 11.beta., 17.alpha., 20, 21-tetrahydroxy steroids of the pregnane class. This method comprises incubating the corresponding 17.alpha., 20.alpha. or .beta., 21-trihydroxy steroid in the presence of a culture medium capable of effecting the 11.beta.-hydroxylation, such medium comprising a fungal culture of the genus Curvularia. Also disclosed herein is a method for preparing 11.beta., 17.alpha., 21-trihydroxy-20-oxo steroids of the pregnane class. This method comprises the hydroxylation method described hereinabove followed by conversion of the resulting 11.beta., 17.alpha., 20.alpha. or .beta., 21-tetrahydroxy steroid into the corresponding 11.beta., 17.alpha., 21-trihydroxy-20-oxo steroid.
    Type: Grant
    Filed: February 6, 1984
    Date of Patent: May 13, 1986
    Assignee: Eastman Kodak Company
    Inventors: Charles T. Goodhue, Gwendolyn C. Kydd, Charles H. Foster, Charles A. McCombs
  • Patent number: 4546078
    Abstract: A biocatalyst contains microorganisms, e.g., of the species Arthrobacter simplex, Aspergillus ochraceus, Bacillus sphaericus, Curvularia lunata, Flavobacterium dehydrogenans, Mycobacterium spec., or Saccharaomyces uvarum, immobilized on a copolymer of acrolein and 1-vinyl-2-pyrrolidone, crosslinked by reaction with an alkylenedioxydiamine of the formulaH.sub.2 NO--(CH.sub.2).sub.n ONH.sub.2wherein n is a number of 2 to 12. The preparation of steroids is also disclosed.
    Type: Grant
    Filed: November 9, 1983
    Date of Patent: October 8, 1985
    Assignee: Schering Aktiengesellschaft
    Inventors: Georg Manecke, Udo Klussmann
  • Patent number: 4528271
    Abstract: Microbiological conversions of cyclodextrins are intensified by adding to the reaction mixture 0.2 to 3 moles of .alpha.-, .beta.- or .gamma.-cyclodextrin or an optical mixture thereof pro mole of the steroid substrate, before, at the beginning or during the conversion. The cyclodextrin if desired, can be removed after the reaction. In this way the reaction velocity can be increased, the reaction time is reduced, the substrate concentration in the solution, i.e. its solubility is increased or the so called product inhibition may be avoided. In certain cases of more alternatives the desired reaction can be catalyzed and in this manner the selectivity is increased.
    Type: Grant
    Filed: September 24, 1982
    Date of Patent: July 9, 1985
    Assignees: Richter Gedeon Vegyeszeti Gyar Rt., Chinoin Gyogyszer- Es Vegyeszeti Termekek Gyara Rt.
    Inventors: Eva Udvardy Nagy nee Cserey Pechany, Istvan Bartho, Gabor Hantos, Maria Trinn, Zsuzsa Vida, Jozsef Szejtli, Agnes Stadler nee Szoke, Ilona Habon, Marta Balazs nee Czurda
  • Patent number: 4353985
    Abstract: A process for preparing an 11.beta.-hydroxy steroid of the formula ##STR1## wherein represents a single bond or a double bond;X is hydrogen, fluorine, chlorine or methyl; andV is methylene, ethylene, ethylidene or vinylidene;comprises fermenting an 11-deoxy steroid of the formula ##STR2## wherein , X and V are as defined above,R.sub.1 is hydrogen or C.sub.1-6 -alkyl; andR.sub.2 is C.sub.1-6 -alkyl;with a fungal culture of a genus Curvularia.
    Type: Grant
    Filed: July 14, 1980
    Date of Patent: October 12, 1982
    Assignee: Schering Aktiengesellschaft
    Inventors: Karl Petzoldt, Klaus Annen, Henry Laurent, Rudolf Wiechert
  • Patent number: 4246346
    Abstract: A method for activating immobilized living microorganisms applied to transformations of steroids, antibiotics and other compounds characterized by the addition of peptone, glucose or a mixture of peptone and glucose to the reaction mixture.
    Type: Grant
    Filed: September 5, 1978
    Date of Patent: January 20, 1981
    Assignee: Aktiebolaget Fermenta
    Inventors: Per-Olof Larsson, Klaus H. Mosbach, Sten A. Ohlson