Preparing Compound Containing A Cyclopentanohydrophenanthrene Nucleus; Nor-, Homo-, Or D-ring Lactone Derivatives Thereof Patents (Class 435/52)
  • Patent number: 10822623
    Abstract: The disclosure provides a metabolic pathway for producing a metabolite, the metabolic pathway having a co-factor purge valve system for recycling a cofactor used in the metabolic pathway.
    Type: Grant
    Filed: January 9, 2019
    Date of Patent: November 3, 2020
    Assignee: The Regents of the University of California
    Inventors: James U. Bowie, Paul H. Opgenorth, Tyler P. Korman
  • Patent number: 10814724
    Abstract: The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.
    Type: Grant
    Filed: April 18, 2019
    Date of Patent: October 27, 2020
    Assignee: The Regents of the University of California
    Inventors: Taek Soon Lee, Aram Kang
  • Patent number: 10400261
    Abstract: The subject of the present invention is a process for preparing a genetically modified yeast by multicopy integration of at least four expression cassettes, allowing the production of a molecule of interest at high titer. The subject of the present invention is also yeasts transformed according to said process, and the use thereof for producing hydrocortisone.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: September 3, 2019
    Assignee: SANOFI
    Inventors: Corinne Brocard-Masson, Isabelle Bonnin, Bruno Dumas
  • Patent number: 10307466
    Abstract: The present invention provides an orally administered composition comprising a sophorolipid, a physiologically active substance, and an oil or fat, wherein the physiologically active substance exhibits an improved bioavailability.
    Type: Grant
    Filed: March 10, 2015
    Date of Patent: June 4, 2019
    Assignee: SARAYA CO., LTD.
    Inventors: Yasushi Suzuki, Hiromitsu Tabata, Mizuyuki Ryu, Nanase Ishii, Takashi Takeuchi, Yasuki Matsumura, Kentaro Matsumiya
  • Patent number: 10273506
    Abstract: The present invention provides for a genetically modified host cell capable of producing isopentenol and/or 3-methyl-3-butenol, comprising (a) an increased expression of phosphomevalonate decarboxylase (PMD) (b) an increased expression of a phosphatase capable of converting isopentenol into 3-methyl-3-butenol, (c) optionally the genetically modified host cell does not express, or has a decreased expression of one or more of NudB, phosphomevalonate kinase (PMK), and/or PMD, and (d) optionally one or more further enzymes capable of converting isopentenol and/or 3-methyl-3-butenol into a third compound, such as isoprene.
    Type: Grant
    Filed: August 21, 2017
    Date of Patent: April 30, 2019
    Assignee: The Regents of the University of California
    Inventors: Taek Soon Lee, Aram Kang
  • Patent number: 9499845
    Abstract: The invention provides methods of cultivating oil-bearing microbes using xylose as a fixed carbon source. Also provided are microorganisms that have been genetically engineered to metabolize xylose as a fixed carbon source allowing them to convert xylose into oils. Particular advantages of the processes provided herein include production of oils rather than alcohols through the microbial fermentation processes utilizing xylose.
    Type: Grant
    Filed: September 1, 2014
    Date of Patent: November 22, 2016
    Assignee: TerraVia Holdings, Inc.
    Inventors: Penelope Chua, Aravind Somanchi
  • Patent number: 9186385
    Abstract: A composition for affecting one or more of influenza, inflammation, and immune system function, said composition comprising an extract prepared from Radix isatidis and an extract prepared from Flos Lonicerae. In alternative embodiments, the composition further comprises one or more of an extract prepared from Poria cocos(Schw.) Wolf and an extract prepared from Herba houttuyniae. Method for treating one or more of influenza, inflammation by administering a patient a therapeutically effective amount of the above composition.
    Type: Grant
    Filed: March 2, 2010
    Date of Patent: November 17, 2015
    Assignee: Viva Pharmaceutical Inc.
    Inventors: Xueju Xie, Jason Jiang-Chung Ko
  • Publication number: 20150093781
    Abstract: The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.
    Type: Application
    Filed: August 13, 2014
    Publication date: April 2, 2015
    Inventors: Michael Schilling, Christine Lang, Andreas Raab
  • Publication number: 20150086613
    Abstract: Disclosed herein are novel lipids and liposomal compositions prepared using such compounds and related methods of neutralizing or otherwise modifying such liposomal compositions. The lipids described herein are useful for example, as liposomal vehicles to facilitate the delivery of encapsulated polynucleotides to target cells and the subsequent transfection of such target cells. In certain embodiments, one or more of the compounds that comprise the liposomal delivery vehicle may be neutralized or further modified such that the properties of the liposomal delivery vehicle are modified.
    Type: Application
    Filed: March 29, 2013
    Publication date: March 26, 2015
    Inventors: Frank DeRosa, Braydon Charles Guild, Michael Heartlein
  • Publication number: 20150031077
    Abstract: The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g.
    Type: Application
    Filed: September 9, 2014
    Publication date: January 29, 2015
    Inventor: Marshall Medoff
  • Publication number: 20140377798
    Abstract: A process for the enzymatic regeneration of the redox cofactors NAD+/NADH and NADP+/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula R1C(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula R2CH(OH)R3 is oxidized and wherein R1, R2 and R3 in the compounds have different meanings.
    Type: Application
    Filed: February 6, 2013
    Publication date: December 25, 2014
    Inventors: Ortwin Ertl, Nicole Staunig, Marta Sut, Bernd Mayer
  • Publication number: 20140329275
    Abstract: In one aspect, the invention provides a recombinant microbial cell modified to exhibit increased biosynthesis of a biosynthetic product compared to a wild-type control. In some cases, the biosynthetic product can be isocaproate; in other cases the biosynthetic product can be isovalerate. In another aspect, the invention provides methods of constructing such recombinant cells. In yet another aspect, the invention provides methods of using the cells to produce the biosynthetic product. In yet another aspect, the invention provides methods of harvesting organic acids from a fermentation culture.
    Type: Application
    Filed: October 9, 2012
    Publication date: November 6, 2014
    Inventor: Kechun Zhang
  • Patent number: 8853168
    Abstract: Avicins and other thioesterification agents are used to modify cysteine groups of signaling proteins in order to modify their activity. Pathologies associated with signaling proteins which are regulatable through this method are treated using these thioesterification agents. These pathologies include those involving proteins which modulate physiologic redox status of organisms including arthersclerosis, cancer, bacterial infection, inflammation, and neurological disorders.
    Type: Grant
    Filed: July 18, 2006
    Date of Patent: October 7, 2014
    Assignee: Duke University
    Inventors: Jonathan S. Stamler, Jordan Gutterman
  • Publication number: 20140186885
    Abstract: The subject of the present invention is a process for preparing a genetically modified yeast by multicopy integration of at least four expression cassettes, allowing the production of a molecule of interest at high titre. The subject of the present invention is also yeasts transformed according to said process, and the use thereof for producing hydrocortisone.
    Type: Application
    Filed: June 18, 2012
    Publication date: July 3, 2014
    Applicant: SANOFI
    Inventors: Corinne Brocard-Masson, Isabelle Bonnin, Bruno Dumas
  • Patent number: 8735137
    Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
    Type: Grant
    Filed: November 15, 2011
    Date of Patent: May 27, 2014
    Assignee: E I du Pont de Nemours and Company
    Inventors: Seung-Pyo Hong, John E. Seip, Quinn Qun Zhu
  • Publication number: 20140134672
    Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.
    Type: Application
    Filed: July 5, 2012
    Publication date: May 15, 2014
    Inventors: Max B. Tuttman, David A. St. Angelo
  • Patent number: 8709753
    Abstract: This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.
    Type: Grant
    Filed: November 19, 2012
    Date of Patent: April 29, 2014
    Assignee: William Marsh Rice University
    Inventors: Ka-Yiu San, George N. Bennett, Yipeng Wang
  • Patent number: 8697393
    Abstract: A fatty material enzyme interesterification process comprising the steps of: a) providing a fatty material that has optionally undergone at least one prior purification treatment; b) introducing soap into said fatty material to form a soap-containing fatty material mixture, c) homogenising said soap-containing fatty material mixture by mixing, d) contacting said soap-containing fatty material mixture with lipase to produce a soap-containing interesterified fatty material, e) removing soaps from said soap-containing interesterified fatty material to yield interesterified fatty material, wherein said soap is either formed in-situ by addition of aqueous alkali to said fatty material or said soap is introduced directly into said fatty material.
    Type: Grant
    Filed: April 16, 2012
    Date of Patent: April 15, 2014
    Assignee: N.V. Desmet Ballestra Engineering S.A.
    Inventors: Marc Kellens, Veronique Gibon, Vaida Petrauskaité, Jeroen Maes
  • Patent number: 8663561
    Abstract: An automated apparatus and method for analyzing liquid samples by forming discrete sample aliquots (boluses) in an elongated conduit which contains a hydrophobic carrier liquid. Aliquots may be analyzed by adding at least one reagent to the sample aliquot that reacts selectively with an analyte contained therein. The reaction product, which is selective for the analyte of interest and proportional to its concentration, is measured with an appropriate detector. Intrinsic sample properties of the sample may also be measured without the need for adding chemical reagents. The invention enables simple and accurate testing of samples using time honored wet-chemical analysis methods in microliter volume regimes while producing remarkably small volumes of waste.
    Type: Grant
    Filed: February 19, 2010
    Date of Patent: March 4, 2014
    Inventor: Charles J. Patton
  • Publication number: 20130331584
    Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.
    Type: Application
    Filed: July 12, 2013
    Publication date: December 12, 2013
    Applicant: Solazyme, Inc.
    Inventors: Scott Franklin, Aravind Somanchi, George Rudenko, Riyaz Bhat, Xinhua Zhao, Risha Bond, Walter Rakitsky, Alejandro Marangoni, Diza Braksmayer
  • Patent number: 8586336
    Abstract: A novel xylose isomerase nucleotide sequence obtained from a bovine rumen fluid metagenomic library and also provides the amino acid sequence encoded by the nucleotide sequence, and a vector and a transformant containing the nucleotide sequence. When the xylose isomerase is expressed, a host cell is endowed with the capability of converting xylose into xylulose, and the xylulose is further metabolized by the host cell. Therefore, the host cell can take the xylose as a carbon source for growth. The xylose isomerase from a new source is expressed with high activity in Saccharomyces cerevisiae and is a mesophilic enzyme with optimal temperature of 60° C.
    Type: Grant
    Filed: June 20, 2011
    Date of Patent: November 19, 2013
    Assignee: Shandong University
    Inventors: Xiaoming Bao, Yu Shen, Ruilei Ge
  • Publication number: 20130224792
    Abstract: The invention relates to novel microbial 7?-hydroxysteroid dehydrogenase (7?-HSDH) knockout mutants and to the use thereof for producing other HSDHs having various functionalities, such as 3?-, 7?- or 12?-HSDH, and to the use of thus-produced HSDH enzymes in enzymatic reactions of cholic acid compounds, and in particular for producing ursodeoxycholic acid (UDCS). The invention relates in particular to novel methods for synthesizing UDCS.
    Type: Application
    Filed: May 27, 2011
    Publication date: August 29, 2013
    Applicant: PharmaZell GmbH
    Inventors: Rolf Schmid, Michael Braun, Luo Liu, Arno Aigner, Dirk Weuster-Botz
  • Publication number: 20130210072
    Abstract: The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
    Type: Application
    Filed: July 13, 2012
    Publication date: August 15, 2013
    Applicant: ORGANOBALANCE GMBH
    Inventors: Christine Lang, Markus Veen
  • Publication number: 20130089892
    Abstract: The present invention relates to yeast species which are normally capable of producing sophorolipids but which are modified in such way that they are incapable producing the latter compounds. These sophorolipid-negative strains surprisingly display equal growth characteristics and biomass formation as their wild type counterparts and are hence useful for the production of compounds such as recombinant proteins, glycolipids, polyhydroxyalkanoates and carotenoides. In addition, the present invention discloses two glucosyltransferase genes with key-functions in sophorolipid production.
    Type: Application
    Filed: June 10, 2011
    Publication date: April 11, 2013
    Applicant: Universiteit Gent
    Inventors: Wim Soetaert, Sofie De Maeseneire, Karen Saerens, Sofie Roelants, Inge Van Bogaert
  • Publication number: 20130084600
    Abstract: This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, native NAD-dependent GAPDH is replaced with NADP-dependent GAPDH plus overexpressed NADK. Uses for the bacteria are also provided.
    Type: Application
    Filed: November 19, 2012
    Publication date: April 4, 2013
    Applicant: WILLIAM MARSH RICE UNIVERSITY
    Inventor: WILLIAM MARSH RICE UNIVERSITY
  • Publication number: 20130084601
    Abstract: The present invention relates to the novel Rhizobium sp. GIN611 KCTC11708BP or to cell extracts thereof, to a novel oxidoreductase which exhibits a glycolytic activity, to a gene encoding the oxidoreductase, to a recombinant strain comprising recombinant vector proteins or to an expression vector encoding recombinant proteins, and to a method for the glycolysis of natural products using same as a biocatalyst. The present invention also relates to a method for producing aglycones from a variety of natural products using same. The novel oxidoreductase separated from the novel microorganism of the present invention does not belong to a glucosidase group but belongs to an oxidoreductase group, and has a glycolytic activity for natural products. The novel oxidoreductase oxidizes the sugar in the aglycones of natural products, thereby producing a variety of aglycones.
    Type: Application
    Filed: June 14, 2011
    Publication date: April 4, 2013
    Applicant: AMOREPACIFIC CORPORATION
    Inventors: Jun Seong Park, Duck Hee Kim, Han Kon Kim, Eun Mi Kim, Byung Gee Kim
  • Publication number: 20130065270
    Abstract: The present invention relates to methods for producing Saccharomyces strains that are capable of growth on xylose as a sole carbon source at a desired growth rate, (such as at least one generation per 48 hours), strains made by such methods, and Saccharomyces strains that grow at a growth rate of at least one generation per 48 hours using xylose as a sole carbon source for growth made by non-recombinant methods.
    Type: Application
    Filed: August 20, 2012
    Publication date: March 14, 2013
    Applicant: Microbiogen Pty Ltd.
    Inventors: Philip John Livingstone Bell, Paul Victor Attfield
  • Patent number: 8367394
    Abstract: The present invention relates to a novel selective synthesis of 9?-hydroxy-steroid derivatives of the general formula (I) (I)—wherein the meaning of -A-A?- is —CH2—CH2— or —CH?CH— group—by the bioconversion of compounds of the general formula (II) (II) wherein the meaning of -A-A?- is —CH2—CH2— or —CH?CH— group—by using Nocardia farcinica bacterium strain, deposition number of which is NCAIM (P)—B 001342, as hydroxylating microorganism in the bioconversion.
    Type: Grant
    Filed: June 30, 2008
    Date of Patent: February 5, 2013
    Assignee: Richter Gedeon Nyrt.
    Inventors: Katalin Olasz, Anikó Tegdes, Valéria Gancsos, Gábor Hantos, Kálmán Könczöl, Gabor Balogh, Sándor Erdélyi
  • Publication number: 20120288891
    Abstract: The present disclosure relates to the use of pantothenate compounds as a non-genetic switch for the production of heterologous acetyl-CoA derived (HACD) compounds in microbial host cells. The invention provides genetically modified microorganisms that are more stable when stored and initially cultured under reduced pantothenate concentrations, cell culture media having reduced concentrations of pantothenate compounds, and methods of producing HACD compounds using the cell culture media and the genetically engineered microorganisms of the invention.
    Type: Application
    Filed: May 9, 2012
    Publication date: November 15, 2012
    Applicant: AMYRIS, INC.
    Inventor: Adam Meadows
  • Publication number: 20120276586
    Abstract: The invention concerns the production of cholesterol of the Fungi kingdom. More particularly, the invention concerns genetically modified Fungus independently producing cholesterol from a simple carbon source. The invention also concerns the use of the inventive Fungus for producing non-marked and marked cholesterol.
    Type: Application
    Filed: May 24, 2012
    Publication date: November 1, 2012
    Applicant: AVENTIS PHARMA S.A.
    Inventors: Denis POMPON, Bruno DUMAS, Roberto SPAGNOLI
  • Publication number: 20120225445
    Abstract: Adipose cells (sebocytes) are described, The invention especially relates to sebaceous gland cells and to a sebaceous gland cell line with the property of being continuously grown over many sub-cultures. The sebocytes are excellently suited for useful applications.
    Type: Application
    Filed: September 29, 2011
    Publication date: September 6, 2012
    Inventor: Christos Zouboulis
  • Publication number: 20120214198
    Abstract: The present invention relates to bioprocess engineering methods in which aqueous phases of anaerobic biologically treated organic suspensions are supplied to algal cultures as media components. The present invention also relates to the use of aqueous phases of anaerobic biologically treated organic suspensions as media components of algal cultures. Additionally, the present invention relates to the use of aqueous phases of anaerobic biologically treated organic suspensions to improve the growth conditions of algae in photobioreactors. Furthermore, the present invention relates to the use of algae for the treatment of aqueous phases of anaerobic biologically treated organic suspensions, in particular of anaerobic biologically treated sewage filtrate. The present invention also relates to bioprocess engineering devices (100) comprising a bioreactor (1), in particular a fermentation tower and a photobioreactor (3).
    Type: Application
    Filed: October 15, 2010
    Publication date: August 23, 2012
    Applicant: FRAUNHOFER-GESELLSCHAFT ZUR FORDERUNG DER ANGEWANDTEN FORSCHUNG E.V.
    Inventor: Walter Trösch
  • Publication number: 20120178124
    Abstract: It is an object of the present invention to obtain highly active P450scc enzyme protein which is an important enzyme protein that catalyzes the first step of the biosynthesis of industrially useful steroid hormone. The present invention provides a sterol side chain cleavage enzyme protein having the following physicochemical properties: (1) action: the enzyme acts on sterol represented by formula (I) as defined in the specification and cleaves the carbon-carbon bond between positions 20 and 22 of a sterol side chain portion by its activity of cleaving the bonds, so as to generate a compound represented by formula (II) as defined in the specification; (2) substrate specificity: when microorganisms that produce the enzyme protein are allowed to react with an aqueous solution containing 100 ?g/ml 4-cholesten-3-one or cholesterol at 28° C.
    Type: Application
    Filed: January 7, 2009
    Publication date: July 12, 2012
    Applicant: MITSUBISHI CHEMICAL CORPORATION
    Inventors: Masahiro Yamagishi, Takeshi Sakamoto, Makoto Ueda, Masashi Itoh, Yoshikazu Fujii, Hiroki Kabumoto, Akira Arisawa, Tadashi Fujii
  • Publication number: 20120171719
    Abstract: Disclosed herein are transformed Yarrowia lipolytica comprising an exogenous polynucleotide encoding a polypeptide having sucrose invertase activity. Also disclosed are methods of using the transformed Y. lipolytica.
    Type: Application
    Filed: November 15, 2011
    Publication date: July 5, 2012
    Applicant: E. I. DU PONT DE NEMOURS AND COMPANY
    Inventors: Seung-Pyo Hong, John E. Seip, Quinn Qun Zhu
  • Publication number: 20120156695
    Abstract: The invention relates to an isolated, genetically modified, living non-mammal organism, having increased HMG-CoA-reductase activity compared to the wild type, and having reduced C24-methyltransferase and/or delta22-desaturase activity compared to the wild type. The invention is characterized in that the organism has increased dehydrocholesterol-delta70-reductase activity compared to the wild type. The invention further relates to different uses of such an organism, to a test kit comprising such an organism, and to a membrane extract of such an organism.
    Type: Application
    Filed: April 1, 2010
    Publication date: June 21, 2012
    Applicant: ORGANOBALANCE GMBH
    Inventors: Michael Schilling, Christine Lang, Andreas Raab
  • Publication number: 20120156717
    Abstract: Recombinant oleaginous alga that include one or more heterologous genes that increase the ability of the alga to use one or more natural saccharides such as cellulosic or hemicellulosic sugars for algal growth are described. The recombinant oleaginous algae are transformed to include one or more genes expressing sugar metabolizing enzymes or sugar transporting proteins, along with suitable control elements. Use of natural saccharides as a carbon source can allow the algae to produce biofuel precursors in a relatively efficient manner. Processes for preparing the alga, growing the alga, and extracting the biofuel precursors from the alga are also described.
    Type: Application
    Filed: August 30, 2010
    Publication date: June 21, 2012
    Applicant: Phycal, Inc.
    Inventors: F.C. Thomas Allnutt, Bradley Lynn Postier, Richard T. Sayre, Daniel A. Coury
  • Patent number: 8198041
    Abstract: A method of producing ?1,4-Tibolone (C21H26O2), 10?-Hydroxy-?4-tibolone (C21H28O3). 11?,15?-Dihydroxytibolone (C21H28O4) and 11?,15?-Dihydroxy-?5-tibolone (C21H28O4) by contacting tibolone and 3?,6?-Dihydroxytibolone (C21H32O3) by contacting 3?-hydroxytibolone with Cunninghamella elegans (ATCC 10028b) is reported.
    Type: Grant
    Filed: January 27, 2011
    Date of Patent: June 12, 2012
    Inventors: Attaur Rahman, Muhammed Iqbal Choudhary, Syed Adnan Ali Shah, Shamsun Nahar Khan
  • Publication number: 20120094328
    Abstract: The present invention provides dendritic cells (DCs) having reduced inflammatory activity, a method for producing DCs capable of inducing regulatory T cells and regulatory T cells, and substances useful for such a method, wherein the DCs and regulatory T cells are useful for prevention and treatment of immune diseases. The present invention relates to a cereal plant-derived material fermented with a koji microorganism, which is obtained by fermentation thereof with a koji microorganism, or a processed product thereof. Preferably, the fermented product or a processed product thereof comprises 14-dehydroergosterol. According to the present invention, regulatory DCs and regulatory T cells can be induced and the proliferation of inflammatory T cells can be suppressed using plant-derived materials.
    Type: Application
    Filed: June 25, 2010
    Publication date: April 19, 2012
    Applicant: KIRIN HOLDINGS KABUSHKIK KAISHA
    Inventors: Daisuke Fujiwara, Masaru Kato, Hideki Koizumi, Kumiko Ikado, Yasuhisa Ano
  • Patent number: 8148556
    Abstract: A new method of producing metabolites of tibolone comprising fermenting tibolone with Rhizopus stolonifer (ATCC 12938) resulting in the formation of ?4-Tibolone (C21H28O2), 6?-Hydroxytibolone, and 15?-Hydroxytibolone (C21H28O3) is reported.
    Type: Grant
    Filed: January 27, 2011
    Date of Patent: April 3, 2012
    Inventors: Attaur Rahman, Muhammed Iqbal Choudhary, Syed Adnan Ali Shah, Shamsun Nahar Khan
  • Publication number: 20120003690
    Abstract: The present invention relates to the modification of lipids in lipid-containing plant material, such as cereal bran for the generation of functional lipids. The present invention further relates to the preparation of compositions comprising such functional lipids as well as the use of these compositions comprising functional lipids and other functional compounds derived from the action of lipid modifying enzymes for the preparation of composition suitable for the preparation of bio-ethanol as well as food products, such as bread.
    Type: Application
    Filed: January 15, 2010
    Publication date: January 5, 2012
    Applicant: Danisco A/S
    Inventors: Jens Frisbaek Sorensen, René Mikkelsen, Charlotte Horsmans Poulsen, Karsten Matthias Kragh
  • Publication number: 20110269181
    Abstract: One embodiment of the present invention discloses a method for continuous biotransformation. The method is continuously supplying viable biocatalyst cells or biocatalyst biomolecules to a bioreactor containing substrate mediums, so as to mediate the substrate mediums to be converted into the desired bioproducts.
    Type: Application
    Filed: April 28, 2010
    Publication date: November 3, 2011
    Applicant: CHUNG YUAN CHRISTIAN UNIVERSITY
    Inventors: CHEAN-YEH CHENG, HSIANG-RONG TSAI
  • Patent number: 8008040
    Abstract: An object of the present invention is to provide a process according to which 5-ene-3-one or 3,6-dione derivatives of sterols can be synthesized with a better yield, as well as a process for production of lipid metabolism improvers, foods, drinks, and animal feeds containing the 5-ene-3-one or 3,6-dione derivatives of sterols. The process for production of 5-ene-3-one or 3,6-dione derivatives of sterols of the present invention involves preparing a culturing solution containing an Arthrobacter bacterium, which is a microorganism exhibiting a cholesterol oxidase activity, and then, adding to the culturing solution an equivalent amount of hexane so as to prepare a bilayer solution consisting of an aqueous layer and a hydrocarbon-based solvent layer. Thereafter, sterol, or a derivative thereof, is made as a substrate contained therein, a reaction performed with cholesterol oxidase at 30° C. for 2 days, and, ethanol then added to separate a hydrocarbon-based solvent layer.
    Type: Grant
    Filed: July 12, 2005
    Date of Patent: August 30, 2011
    Assignee: Toyo Hakko Co., Ltd.
    Inventors: Kunio Suzuki, Tadashi Nagashima, Shinya Nagahashi
  • Publication number: 20110207171
    Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.
    Type: Application
    Filed: January 14, 2011
    Publication date: August 25, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Brian Agnew, Schuyler B. CORRY, Kyle R. GEE
  • Publication number: 20110191875
    Abstract: The invention provides compositions and methods for producing androstenedione (4-androstenedione), of improved purity and for modulating its production, for example by deletion or inactivation of ksdA, cxgA, cxgB, cxgC, or cxgD. The invention also provides methods and compositions, including nucleic acids that encode enzymes, for producing 1,4-androstadiene-3,17-dione (ADD) and related pathway compounds, including 20-(hydroxymethyl)pregna-4-en-3-one and 20-(hydroxymethyl)pregna-1,4-dien-3-one. The compositions of the invention include nucleic acids, probes, vectors, cells, transgenic plants and seeds, transgenic animals, kits and arrays.
    Type: Application
    Filed: November 13, 2008
    Publication date: August 4, 2011
    Applicant: Verenium Corporation
    Inventors: David Nunn, Catherine Pujol, Kelly Chatman
  • Patent number: 7977065
    Abstract: The present invention relates to genetically modified yeast strains autonomously producing, from a simple carbon source, steroids. The invention also relates to a method for producing steroids from such yeast strains.
    Type: Grant
    Filed: January 7, 2010
    Date of Patent: July 12, 2011
    Assignee: Aventis Pharma S.A.
    Inventors: Roberto Spagnoli, Tilman Achstetter, Gilles Cauet, Eric Degryse, Bruno Dumas, Denis Pompon, Jacques Winter
  • Publication number: 20110151509
    Abstract: The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves.
    Type: Application
    Filed: October 27, 2009
    Publication date: June 23, 2011
    Applicant: ORGANOBALANCE GMBH
    Inventors: Christine Lang, Markus Veen
  • Publication number: 20110124044
    Abstract: A method of producing ?1,4-Tibolone (C21H26O2), 10?-Hydroxy-?4-tibolone (C21H28O3). 11?,15?-Dihydroxytibolone (C21H28O4) and 11?,15?-Dihydroxy-?5-tibolone (C21H28O4) by contacting tibolone and 3?,6?-Dihydroxytibolone (C21H32O3) by contacting 3?-hydroxytibolone with Cunninghamella elegans (ATCC 10028b) is reported.
    Type: Application
    Filed: January 27, 2011
    Publication date: May 26, 2011
    Applicant: INTERNATIONAL CENTER FOR CHEMICAL SCIENCES
    Inventors: Attaur Rahman, Mohammed Iqbal Choudhary, Syed Adnan Ali Shah, Shamsun Nahar Khan
  • Publication number: 20110124043
    Abstract: Method of producing ?5-Tibolone (C21H28O2), 6?-Hydroxy-?4-tibolone (C21H28O3), 6?-Hydroxy-?4-tibolone (C21H28O3), 15?-Hydroxy-?4-tibolone (C21H28O3), 6?-Hydroxy-?1,4-tibolone (C21H28O3) and 6?-Methoxy-?4-tibolone (C22H30O3) contacting tibolone with Gibberella fujikuroi (ATCC 10704) is reported.
    Type: Application
    Filed: January 27, 2011
    Publication date: May 26, 2011
    Applicant: INTERNATIONAL CENTER FOR CHEMICAL SCIENCES
    Inventors: Attaur Rahman, Mohammad Iqbal Choudhary, Syed Adnan Ali Shah, Shamsun Nahar Khan
  • Publication number: 20110124041
    Abstract: A new method of producing metabolites of tibolone comprising fermenting tibolone with Rhizopus stolonifer (ATCC 12938) resulting in the formation of ?4-Tibolone (C21H28O2), 6?-Hydroxytibolone, and 15?-Hydroxytibolone (C21H28O3) is reported.
    Type: Application
    Filed: January 27, 2011
    Publication date: May 26, 2011
    Applicant: INTERNATIONAL CENTER FOR CHEMICAL SCIENCES
    Inventors: Attaur Rahman, Mohammed Iqbal Choudhary, Syed Adnan Ali Shah, Shamsun Nahar Khan
  • Patent number: RE43979
    Abstract: The invention relates to a method for detecting specific target-cells in a simple and time saving way, using paramagnetic particles, antibodies recognizing the Fc portions of target-cell associating antibodies and target-cell associating antibodies directed to specific antigen determinants in the target-cell membranes. Incubation of the cell suspension with a mild detergent and/or second set of antibodies or antibody fragments, prelabeled or not with fluorescent agents, metallocolloids, radioisotopes, biotincomplexes or certain enzymes allowing visualization, with dramatically increase the specificity of the method. The method can further be used for isolation of the target-cells by magnetic field application and kit for performing the method according to the invention is described.
    Type: Grant
    Filed: February 5, 2003
    Date of Patent: February 5, 2013
    Assignee: Abbott Laboratories
    Inventors: Oystein Fodstad, Gunnar Kvalheim