Involving Nucleic Acid Patents (Class 435/6.1)
  • Patent number: 10144961
    Abstract: This invention provides novel azido linkers for deoxynucleotide analogs having a detectable marker attached thereto.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: December 4, 2018
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Jingyue Ju, Huanyan Cao, Zengmin Li, Qinglin Meng, Jia Guo, Shenglong Zhang, Lin Yu
  • Patent number: 10138458
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: November 27, 2018
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phillip Belgrader, Benjamin Hindson
  • Patent number: 10138514
    Abstract: Some embodiments described herein relate to modified nucleotide and nucleoside molecules with novel 3?-hydroxy protecting groups. Also provided herein are methods to prepare such modified nucleotide and nucleoside molecules and sequencing by synthesis processes using such modified nucleotide and nucleoside molecules.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: November 27, 2018
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, Xiaolin Wu, Geoffrey Paul Smith
  • Patent number: 10131936
    Abstract: According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: November 20, 2018
    Assignee: Life Technologies Corporation
    Inventors: Scott C. Benson, Cinna Monighetti, Sandy M. Koepf
  • Patent number: 10131902
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: November 20, 2018
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Carol Kreader
  • Patent number: 10131890
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: November 20, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
  • Patent number: 10127348
    Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: November 13, 2018
    Assignee: Life Technologies Corporation
    Inventors: Gordon A. Janaway, Evelyn Wing-Sim Chan
  • Patent number: 10125367
    Abstract: The present invention relates to a pharmaceutical composition for preventing or treating atopic dermatitis, the pharmaceutical composition including, as an active ingredient, X-shaped DNA (XL-DNA) formed by complementary binding of oligonucleotides having nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 4. When the pharmaceutical composition is subcutaneously injected into an animal model of atopic dermatitis, effects of easing skin lesions, such as erythema, bleeding and edema, and the like, and ear edema, and reducing expression of immunoglobulin E (IgE) are exhibited. In this regard, the composition can be used as a pharmaceutical composition, a health food, or a cosmetic for atopic dermatitis.
    Type: Grant
    Filed: April 6, 2016
    Date of Patent: November 13, 2018
    Assignee: THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Joo Young Lee, Gabsik Yang
  • Patent number: 10125364
    Abstract: The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ALAS1 gene, and methods of using such dsRNA compositions to alter (e.g., inhibit) expression of ALAS1.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: November 13, 2018
    Assignees: ALYNYLAM PHARMACEUTICALS, INC., ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
    Inventors: Brian Bettencourt, Kevin Fitzgerald, William Querbes, Robert J. Desnick, Makiko Yasuda
  • Patent number: 10119163
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: November 6, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Patent number: 10119143
    Abstract: The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ALAS1 gene, and methods of using such dsRNA compositions to alter (e.g., inhibit) expression of ALAS1.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: November 6, 2018
    Assignees: ALNYLAM PHARMACEUTICALS, INC., ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
    Inventors: Brian Bettencourt, Kevin Fitzgerald, William Querbes, Robert J. Desnick, Makiko Yasuda
  • Patent number: 10119162
    Abstract: Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5? or 3? flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5? and 3? flaps are generated. The flaps are cleaved using 5? or 3? flap endonucleases or 3? to 5? exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: November 6, 2018
    Assignee: AFFYMETRIX, INC.
    Inventors: Jianbiao Zheng, Li Weng, Malek Faham
  • Patent number: 10113172
    Abstract: The present invention relates to optimized aptamers and methods of using these aptamers.
    Type: Grant
    Filed: April 17, 2017
    Date of Patent: October 30, 2018
    Assignee: University of Iowa Research Foundation
    Inventors: Paloma Giangrande, Francis Miller, William Thiel
  • Patent number: 10113203
    Abstract: The present invention relates to a kit and nucleic acid chip for diagnosing bladder cancer using a bladder cancer-specific marker gene. More particularly, the invention relates to a kit and nucleic acid chip for diagnosing bladder cancer, which can detect the promoter methylation of a bladder cancer-specific gene, the promoter or exon region of which is methylated specifically in transformed cells of bladder cancer. The use of the diagnostic kit or nucleic acid chip of the invention enables diagnosis of bladder cancer at an early stage of transformation, thus enabling early diagnosis of bladder cancer, and can diagnose bladder cancer in a more accurate and rapid manner compared to a conventional method.
    Type: Grant
    Filed: September 19, 2017
    Date of Patent: October 30, 2018
    Assignee: GENOMICTREE, INC.
    Inventors: Sung Whan An, Young Ho Moon, Tae Jeong Oh
  • Patent number: 10115034
    Abstract: Systems and methods generate a projected image at an optimal exposure time. Images are captured different exposure times. Pixels that satisfy an intensity threshold percentage for each image are selected. The intensity values of the selected pixels are then evaluated to determine whether the selected pixels are distributed above a lower intensity threshold and below an upper intensity threshold. The linear relationship is projected to determine an optimal exposure time that has an optimal exposure time duration that exceeds each exposure time duration associated with each of the captured images when the linear relationship exists between each of the captured images. A projected image associated with the optimal exposure time is generated from one or more of the captured images.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: October 30, 2018
    Assignees: LIFE TECHNOLOGIES HOLDINGS PTE LIMITED, PIERCE BIOTECHNOLOGY, INC.
    Inventors: Suk Hong, Nikki Jarrett, Eric Hommema, Jason Aravich, Yanpeng Cao, Benyong Shi, Kok Siong Teo
  • Patent number: 10116490
    Abstract: A method for use by an apparatus of a communication network comprises detecting (S22) that a user or user equipment attaching to the communication network belongs to a usage group, based on group identity information that is allocated to the user or user equipment and identifies the usage group, detecting (S24), based on the group identity information, whether or not a first rules function out of several rules functions has been allocated to another user or user equipment of the usage group, and in case it is detected that the first rules function has been allocated to another user or user equipment of the usage group, selecting (S26), for the user or user equipment, the first rules function.
    Type: Grant
    Filed: September 7, 2012
    Date of Patent: October 30, 2018
    Assignee: NOKIA SOLUTIONS AND NETWORKS OY
    Inventor: Juha Antero Rasanen
  • Patent number: 10106838
    Abstract: Composition, method and kit of parts for a simplified and safe extraction of nucleic acids from biological samples, such as crude and processed food, and subsequent analysis by polymerase chain reaction to the presence of animal material, genetically modified organisms, allergens and pathogens. The method comprises the addition of extracting composition together with hot water for extraction and stabilization of nucleic acids as well as removal of substances interfering with DNA polymerase activity.
    Type: Grant
    Filed: March 3, 2015
    Date of Patent: October 23, 2018
    Assignee: IFP PRIVATES INSTITUT FÜR PRODUKTQUALITÄT GMBH
    Inventors: Wolfgang Weber, Christine Werner
  • Patent number: 10106850
    Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
    Type: Grant
    Filed: August 22, 2017
    Date of Patent: October 23, 2018
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
  • Patent number: 10105674
    Abstract: The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: October 23, 2018
    Assignee: Genomics USA, Inc.
    Inventor: Michael E. Hogan
  • Patent number: 10100291
    Abstract: Methods of making deletion mutants of Cas9 proteins and making chimeric Cas9 proteins are described. The Cas9 N- and C-terminal domains may play critical roles in crRNAitracrRNA binding and/or PAM selectivity. To analyze activity, a series of domain exchange mutants between NM and STI (Streptococcus thermophilus Cas9) were made, replacing the N and/or C terminus of NM (Neisseria meningitides Cas9) with the homologous region from STI. The chimeric proteins were then tested using the transcriptional reporter assay described herein altering the guideRNA and/or Cas9 specific PAM within the reporter to determine the influence of the domain exchanges on protein specificity. None of the N-terminal domain swaps between NM and STI endowed NM with novel properties. The C-terminal exchange generated a NM-STI hybrid that was capable of interacting with the STI crRNAitracrRNA complex and was further able to suppress a reporter with a STI specific PAM.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: October 16, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Alejandro Chavez, Frank Poelwijk, George M. Church
  • Patent number: 10100349
    Abstract: Methods and compositions for determining the presence of a polymorphism at a target nucleotide position in a plurality of target nucleic acid sequences is provided.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: October 16, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Iain J. Macleod, Christopher F. Rowley, Max Essex
  • Patent number: 10100365
    Abstract: The invention disclosed herein is based on the identification of novel mutations in the JAK2 gene and JAK2 protein. The invention provides compositions and methods useful for diagnosing hematopoietic diseases including, for example, myeloproliferative diseases. The invention also provides compositions and methods useful for determining a prognosis of an individual diagnosed as having a hematopoietic disease.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: October 16, 2018
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Maher Albitar, Wanlong Ma
  • Patent number: 10093962
    Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: October 9, 2018
    Assignee: Agilent Technologies, Inc.
    Inventor: Michael Borns
  • Patent number: 10087481
    Abstract: Methods and compositions are provided for enriching for target sequences from a population of nucleic acids, that includes: combining in solution, a population of nucleic acids and a target isolation probe wherein the target isolation probe comprises an affinity binding domain; permitting a single stranded region of the target isolation probe to hybridize to all or a portion of a target sequence in the population of nucleic acids; selectively immobilizing the hybridized nucleic acids from the population containing the target sequences by associating the target isolation probe with a capture domain and removing unbound material; removing non-target sequences from the 3? end of the target sequence by means of one or more 3? exonucleases thereby generating a blunt ended duplex or a staggered end at the 3? end of the target sequence; optionally ligating a 3? duplex adaptor or a duplex end of a hairpin adaptor to the 3? end of the target sequence and the 5? end of the target isolation probe; extending the 3? end o
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: October 2, 2018
    Assignee: New England Biolabs, Inc.
    Inventor: Cynthia Richard
  • Patent number: 10087445
    Abstract: The invention pertains to modifications for antisense oligonucleotides, wherein the modifications are used to improve stability and provide protection from nuclease degradation. The modifications could also be incorporated into double-stranded nucleic acids, such as synthetic siRNAs and miRNAs.
    Type: Grant
    Filed: November 23, 2016
    Date of Patent: October 2, 2018
    Assignee: Integrated DNA Technologies, Inc.
    Inventors: Mark Aaron Behlke, Richard Owczarzy, Yong You, Joseph Alan Walder, Kim Lennox
  • Patent number: 10087488
    Abstract: The described invention relates to the identification of biomarkers for gastrointestinal diseases and provides methods utilizing the biomarkers for in drug discovery, monitoring of treatment efficacy, and diagnostics. The invention further provides methods for identifying a therapeutic target to treat ulcerative colitis, colorectal cancer, and Crohn's disease.
    Type: Grant
    Filed: February 22, 2016
    Date of Patent: October 2, 2018
    Assignee: AlfaGene Bioscience, Inc.
    Inventor: Asit Panja
  • Patent number: 10087448
    Abstract: There is described herein compounds, compositions and methods for inducing synthetic lethality in a cancer cell(s).
    Type: Grant
    Filed: June 30, 2015
    Date of Patent: October 2, 2018
    Assignees: THE GOVERNORS OF THE UNIVERSITY OF ALBERTA, ALBERTA HEALTH SERVICES
    Inventors: Michael Weinfeld, Todd Randall Mereniuk, Edan Foley, Dennis G. Hall
  • Patent number: 10085230
    Abstract: A system and method for determining the location of a target mobile device in a communications network. A set of mobile devices in proximity to the target mobile device may be determined (210) as a function of a common parameter of information observed by mobile devices in the set. Measurement data may be shared (220) for the set of mobile devices and the target mobile device, and a location of the target mobile device (230) determined as a function of the shared measurement data. This sharing of measurement data may occur amongst the set of mobile devices and the target mobile device or may also occur at a communication network entity.
    Type: Grant
    Filed: May 24, 2017
    Date of Patent: September 25, 2018
    Assignee: CommScope Technologies LLC
    Inventors: Martin C. Alles, Thomas B. Gravely, Khalid W. Al-Mufti, Andrew E. Beck
  • Patent number: 10081598
    Abstract: The present invention provides a cationic lipid which can be utilized for nucleic acid delivery into the cytoplasm. The cationic lipid of the present invention is, for example, a compound represented by the following Formula (1) or a pharmaceutically acceptable salt thereof.
    Type: Grant
    Filed: December 24, 2015
    Date of Patent: September 25, 2018
    Assignees: Eisai R&D Management Co., Ltd., Sogo Pharmaceutical Co., Ltd.
    Inventors: Yuta Suzuki, Kenji Hyodo, Yohei Tanaka
  • Patent number: 10083340
    Abstract: The disclosed subject matter relates to an automated determination of cell-by-cell segmentation quality of a tissue specimen sample. A training set of cells is examined by an expert to determine which cells that include “good” segmentation and which cells include “poor” segmentation. A training model is build based on the image data of the cells in the training set. Image data from cells in a test specimen is obtained and that image data is compared to the training model to determine on a cell-by-cell basis which cells in the test specimen include “good” segmentation and which cells include “poor” segmentation. The accumulated data on the cells analyzed in the test specimen can be utilized to determine an overall segmentation quality score for the area of the test specimen in which the individual cells are located in the test specimen.
    Type: Grant
    Filed: January 26, 2016
    Date of Patent: September 25, 2018
    Assignee: GE Healthcare Bio-Sciences Corp.
    Inventors: Raghav Krishna Padmanabhan, Edward John Moler, Yousef Al-Kofahi, Alberto Santamaria-Pang, Brion Daryl Sarachan, Qing Li
  • Patent number: 10081834
    Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure.
    Type: Grant
    Filed: January 22, 2014
    Date of Patent: September 25, 2018
    Assignee: DYNAMIC BIOSENSORS GMBH
    Inventors: Andreas Langer, Ralf Strasser, Ulrich Rant
  • Patent number: 10084551
    Abstract: A method for molecular communication within a nanonetwork. The method includes the steps of: (i) providing a nanonetwork communication system including a transmitter, a receiver, and a physical channel layer, the physical channel layer including a medium configured to allow a communication molecule to be transmitted from the transmitter to the receiver; (ii) transmitting, via the physical channel layer, the communication molecule from the transmitter in a plurality of bursts, wherein information is encoded in the concentration of the communication molecule; (iii) receiving the communication molecule by the receiver; and (iv) varying at least one of the plurality of bursts, wherein varying the plurality of bursts modulates the information transmitted by the communication molecule.
    Type: Grant
    Filed: February 23, 2017
    Date of Patent: September 25, 2018
    Assignee: International Business Machines Corporation
    Inventors: Sushovan Das, Soumallya Chatterjee, Amitava Mukherjee, Mrinal K. Naskar
  • Patent number: 10077463
    Abstract: Provided herein are photochemical crosslinkers and photocleavable crosslinkers and their uses in methods for cell selection from cell cultures. The photochemical crosslinkers comprise a fluorescent dye and a radical generator. The photocleavable crosslinkers comprise a photocleavable linker linking two electrophilic groups to each other. Also provided are systems for imaging cells comprising a plurality of cells crosslinked to extracellular matrix proteins using a crosslinker as described, an imaging apparatus, an illuminating apparatus, and software for image processing.
    Type: Grant
    Filed: January 14, 2016
    Date of Patent: September 18, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Adam E. Cohen, Miao-Ping Chien
  • Patent number: 10072304
    Abstract: A method for quantitatively evaluating chromatin structural changes using pixel imaging of the nucleus is provided. Pixel imaging of the nucleus can include capturing one or more images of a nucleus of one or more nucleic acid stain treated cells. The stain intensity can be measured by quantitating the intensity. The mean and/or standard deviation of stain intensity per pixel can be used to determine chromatin condensation levels or chromatin structural change.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: September 11, 2018
    Assignee: National University Corporation Chiba University
    Inventor: Naoto Yamaguchi
  • Patent number: 10064963
    Abstract: The present disclosure relates to a method of treating, ameliorating or preventing a disorder by blocking the signaling pathway of c-Met, wherein said method comprises the step of administering a therapeutically effective amount of a composition to a subject in need thereof, which composition contains a lipocalin mutein or a fragment or a variant thereof. In addition, the present disclosure relates to a diagnostic composition suitable for use in, e.g., an immuno-imaging technique to detect the presence of c-Met as well as the uses thereof.
    Type: Grant
    Filed: January 7, 2013
    Date of Patent: September 4, 2018
    Assignee: PIERIS PHARMACEUTICALS GMBH
    Inventors: Shane Olwill, Hendrik Gille, Laurent Audoly, Marlon Hinner, Elisabeth De Vries, Anton G. T. Terwisscha Van Scheltinga
  • Patent number: 10067138
    Abstract: Provided are a fluorescent particle and a method for manufacturing the same. The fluorescent particle may include a gold nanoparticle; a silica shell covering the gold nanoparticle; and lanthanide group complex particles dispersed in the silica shell. Each of the lanthanide group complex particles may include a lanthanide group ion; a ligand bonded to the lanthanide group ion and including phosphorus; and a ligand bonded to the lanthanide group ion and having a beta diketone functional group. The fluorescent particle is observable with the naked eye and may emit light when ultraviolet light is irradiated. The fluorescent particle may be used for detecting and analyzing biomaterial samples.
    Type: Grant
    Filed: August 18, 2014
    Date of Patent: September 4, 2018
    Assignee: MEDISENSOR, INC.
    Inventors: So yeon Kim, Nae Young Heo, Jeaan Jung, Won Jung Kim, Byung Gap Hwang, Young Seop Kim, Myoung Hoon Lee
  • Patent number: 10053742
    Abstract: Disclosed are nucleic acid oligomers, including amplification oligomers, capture probes, and detection probes, for detection of Hepatitis E Virus (HEV) nucleic acid. Also disclosed are methods of specific nucleic acid amplification and detection using the disclosed oligomers, as well as corresponding reaction mixtures and kits.
    Type: Grant
    Filed: August 14, 2014
    Date of Patent: August 21, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Kui Gao, Edgar O. Ong, Jennifer Cole, Jeffrey M. Linnen
  • Patent number: 10049561
    Abstract: Example implementations relate to a fiber-shaped device for transmitting a distress signal to a remote computer. The fiber-shaped device includes a sensor, an analysis module that determines if a sensor signal from the sensor includes a trigger signal, and a wireless communication module to transmit a distress signal to a remote computer when the sensor signal includes the trigger signal.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: August 14, 2018
    Assignee: ENT. SERVICES DEVELOPMENT CORPORATION LP
    Inventor: Satwant Kaur
  • Patent number: 10049183
    Abstract: A method and an apparatus for analyzing a biosignal to respond to an infectious disease are provided. An analyzing method involves receiving biosignals of users of terminals and location information of the terminals, determining infectiousness of a condition of a user among the users by analyzing the location information and the biosignals, and transmitting feedback information regarding the infectiousness of the condition to one or more of the terminals.
    Type: Grant
    Filed: July 11, 2016
    Date of Patent: August 14, 2018
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Seungwoo Noh, Younho Kim, Sangyun Park
  • Patent number: 10047407
    Abstract: Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed.
    Type: Grant
    Filed: June 23, 2015
    Date of Patent: August 14, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Sylvia A. Norman, Jennifer J. Bungo, William L. Hanna, Neeraj P. Rao
  • Patent number: 10041933
    Abstract: The present invention relates to modulators of the interaction of astrin and raptor, and their uses in the treatment of mTOR related diseases, such as cancer.
    Type: Grant
    Filed: January 13, 2014
    Date of Patent: August 7, 2018
    Assignee: ALBERT-LUDWIGS-UNIVERSITÄT FREIBURG
    Inventors: Kathrin Thedieck, Birgit Holzwarth, Ralf Baumeister
  • Patent number: 10041117
    Abstract: The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
    Type: Grant
    Filed: July 11, 2014
    Date of Patent: August 7, 2018
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Irina Nazarenko, Ayoub Rashtchian, Joseph Solus, Richard M. Pires, Marlene Darfler, Gulilat Gebeyehu, Mekbib Astatke
  • Patent number: 10041939
    Abstract: The present invention relates to a cartridge (1) for the detection of an analyte (2) in a binding assay with magnetic labels (3), the cartridge comprising at least one capture binding agent (4) against a binding site on the analyte and comprising at least two magnetically labelled tracer binding agents (51 and 52) against further different binding sites on the analyte, characterized in that the cartridge comprises at least two regions (61 and 62) wherein a first region (61) comprises the at least one capture binding agent (4) and a first magnetically labelled tracer binding agent (51) and wherein a second region (62) comprises at least one capture binding agent (4) and a second magnetically labelled tracer binding agent (52).
    Type: Grant
    Filed: September 10, 2010
    Date of Patent: August 7, 2018
    Assignee: Minicare B.V.
    Inventors: Jeroen Hans Nieuwennhuis, Toon Hendrik Evers
  • Patent number: 10041110
    Abstract: The invention provides improved methods for synthesizing polynucleotides, such as DNA and RNA, using enzymes and specially designed nucleotide analogs. Using the methods of the invention, specific sequences of polynucleotides can be synthesized de novo, base by base, in an aqueous environment, without the use of a nucleic acid template. Because the nucleotide analogs have an unmodified 3? OH, i.e., as found in “natural” deoxyribose and ribose molecules, the analogs result in natural polynucleotides suitable for incorporation into biological systems.
    Type: Grant
    Filed: July 28, 2014
    Date of Patent: August 7, 2018
    Assignee: Molecular Assemblies, Inc.
    Inventors: J. William Efcavitch, Suhaib Siddiqi
  • Patent number: 10036055
    Abstract: Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.
    Type: Grant
    Filed: May 22, 2014
    Date of Patent: July 31, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Kun Zhang, Joseph Chou
  • Patent number: 10036743
    Abstract: The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: July 31, 2018
    Inventors: Roger Giese, Poguang Wang
  • Patent number: 10030261
    Abstract: The present invention relates to methods and products for the localized or spatial detection of nucleic acid in a tissue sample and in particular to a method for localized detection of nucleic acid in a tissue sample comprising: (a) providing an array comprising a substrate on which multiple species of capture probes are directly or indirectly immobilized such that each species occupies a distinct position on the array and is oriented to have a free 3? end to enable said probe to function as a primer for a primer extension or ligation reaction, wherein each species of said capture probe comprises a nucleic acid molecule with 5? to 3?: (i) a positional domain that corresponds to the position of the capture probe on the array, and (ii) a capture domain; (b) contacting said array with a tissue sample such that the position of a capture probe on the array may be correlated with a position in the tissue sample and allowing nucleic acid of the tissue sample to hybridize to the capture domain in said capture probes;
    Type: Grant
    Filed: April 13, 2012
    Date of Patent: July 24, 2018
    Assignee: SPATIAL TRANSCRIPTOMICS AB
    Inventors: Jonas Frisen, Patrik Ståhl, Joakim Lundeberg
  • Patent number: 10023605
    Abstract: Compositions, methods, and systems are provided for nucleotide analogs comprising protein shields for improving enzyme photostability in single molecule real time sequencing. Nucleotide analogs of the invention have a protein shield between the dye moieties and nucleotide moieties of the analog. The nucleotide analogs have two avidin proteins connected by a central dye component, and each avidin protein is further connected to a nucleotide component. The protein can prevent the direct interaction of the dye moiety with the enzyme carrying out nucleotide synthesis preventing photodamage to the enzyme. The nucleotide analogs of the invention can have multiple dyes and multiple nucleotide moieties.
    Type: Grant
    Filed: June 1, 2015
    Date of Patent: July 17, 2018
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Keith Bjornson, Jeremiah Hanes, Erik Miller, Satwik Kamtekar, Lubomir Sebo, Louis Brogley
  • Patent number: 10023868
    Abstract: The invention relates to the diagnostic and therapeutic uses of a miRNA molecule, an equivalent or a source thereof in a disease and condition associated with neo-angiogenesis.
    Type: Grant
    Filed: August 3, 2016
    Date of Patent: July 17, 2018
    Assignee: INTERNA TECHNOLOGIES B.V.
    Inventors: Sanne Weijzen, Roeland Quirinus Jozef Schaapveld, Meriem Bourajjaj, Rick Jan Van Haastert, Arjan Willem Griffioen, Judith Rosina Van Beijnum, Edwin Pieter Johan Gerard Cuppen, Eugene Berezikov, Andreas Alphons Franciscus Ludovicus Van Puijenbroek, Willemijn Maria Gommans, Negar Babae, Petronella Innocentia Van Noort
  • Patent number: 10023911
    Abstract: Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof.
    Type: Grant
    Filed: October 13, 2015
    Date of Patent: July 17, 2018
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Austin B. Tomaney, Kenneth Mark Maxham, David Holden, Kevin Hester, Devon Murphy, Patrick Marks