Involving Nucleic Acid Patents (Class 435/6.1)
  • Patent number: 10208339
    Abstract: Provided herein are systems and methods for whole genome amplification and sequencing. In particular, provided herein are systems and methods for detection of nucleic acid variants (e.g., rare variants) in limited samples.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: February 19, 2019
    Assignee: TAKARA BIO USA, INC.
    Inventors: Alain-Albert Mir, Thomas David Schaal, Jude Dunne, Maithreyan Srinivasan
  • Patent number: 10208341
    Abstract: The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.
    Type: Grant
    Filed: May 2, 2014
    Date of Patent: February 19, 2019
    Assignee: Life Technologies Corporation
    Inventors: Peter Williams, Daniel J. B. Williams, Linda Bloom, Thomas J. Taylor
  • Patent number: 10201557
    Abstract: Compositions, kits, cells and methods for treating cardiovascular (e.g., myocardial ischemia and heart failure), immunological, and inflammatory diseases or disorders involve the use of the mature and precursor sequences of microRNAs 142-5p, 142-3p, 17-5p, 17-3p, 374, and 20a, and of antisense molecules complementary to these sequences, to manipulate processes relevant to, for example, the cardiac response to stress, including survival signaling, angiogenesis, stem cell differentiation along muscle or vascular lineages, and repression or promotion of cardiac myocyte growth. Also described are methods to treat cardiovascular, immunological and inflammatory diseases by engineering cells containing specific micro-RNAs or antagomirs against specific mRNAs. The engineered cells can then be used to treat patients with such diseases by autologous stem cell therapy.
    Type: Grant
    Filed: February 1, 2016
    Date of Patent: February 12, 2019
    Assignee: UNIVERSITY OF MIAMI
    Inventors: Nanette Bishopric, Salil Sharma
  • Patent number: 10203334
    Abstract: Small molecule fluorescent probes for established drug targets such as nucleic acids including DNA and RNA has been developed and disclosed herein. These nucleic acid probes bind to multiple DNA and RNA structures, and to sites crucial for nucleic acid function, such as DNA and RNA major grooves. Displacement of the probes by other binders such as small molecule compounds and/or proteins illicits a fluorescence change in the probe that once detected and analyzed provide binding information of these other binders of interest. Similarly, changes in fluorescence upon binding of the probes to nucleic acid have been applied to screen nucleic acid of different sequence and conformation. The nucleic acid probes and method of uses disclosed herein are advantageously suitable for high-through put screening of libraries of small molecule compounds, proteins, and nucleic acids.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: February 12, 2019
    Assignee: Nubad, LLC
    Inventors: Dev P. Arya, Frank Anderson Norris, Jason Derrick Watkins
  • Patent number: 10203335
    Abstract: Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system.
    Type: Grant
    Filed: February 9, 2017
    Date of Patent: February 12, 2019
    Assignees: The Trustees of the University of Pennsylvania, Anima Cell Metrology
    Inventors: Barry S. Cooperman, Zeev Smilansky, Yale E. Goldman, Dongli Pan
  • Patent number: 10202628
    Abstract: Methods and compositions for synthesizing nucleic acid sequences in an emulsion are provided.
    Type: Grant
    Filed: February 14, 2013
    Date of Patent: February 12, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: George M. Church, Richard C. Terry, Sriram Kosuri, Di Zhang
  • Patent number: 10196683
    Abstract: The present invention relates to a method of preparation of substrates for nucleic acid sequencing reactions. More specifically, the present invention provides a new method of preparing hairpins using force-induced strand invasion. Hairpins prepared by this method and methods of nucleic acid analysis using these hairpins are also part of the present invention.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: February 5, 2019
    Assignees: PARIS SCIENCES ET LETTRES—QUARTIER LATIN, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (cnrs), SORBONNE UNIVERSITE, UNIVERSITE PARIS DIDEROT PARIS 7
    Inventors: Vincent Croquette, Jimmy Ouellet
  • Patent number: 10196673
    Abstract: Improved compositions for and methods of processing and analyzing samples are described. In particular, the compositions and methods liberate nucleic acids from a biological sample allowing direct downstream processing of the nucleic acids in microfluidic systems. These compositions, methods and kits are useful in diagnosing, staging or otherwise characterizing various biological conditions.
    Type: Grant
    Filed: February 18, 2014
    Date of Patent: February 5, 2019
    Assignee: BIOCARTIS N.V.
    Inventors: Koen Van Acker, Bart Claes, Benoit Devogelaere, Geert Maertens, Erwin Sablon, Pascale Holemans, Tania Ivens
  • Patent number: 10190157
    Abstract: Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith.
    Type: Grant
    Filed: August 7, 2015
    Date of Patent: January 29, 2019
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaolin Wu, Xiaohai Liu
  • Patent number: 10190120
    Abstract: Provided herein are a novel Zn-DPA complex compound and an siRNA delivery system including the same as a transporter, the Zn-DPA complex compound including: a phosphate-directing functional part of zinc (II)-dipicolylamine (“Zn-DPA”); a cell membrane-directing functional part; and a linker part that links the phosphate-directing functional part and the cell membrane-directing functional part. The Zn-DPA complex compound has low toxicity and efficiently delivers siRNA to cells, thereby useful in various ways for various studies and diagnosis and treatment of diseases, which use siRNA.
    Type: Grant
    Filed: August 8, 2017
    Date of Patent: January 29, 2019
    Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY
    Inventors: Gyo Chang Keum, Eun Kyoung Bang, Jin Bum Kim
  • Patent number: 10190178
    Abstract: Methods for the rapid detection of the presence or absence of mecC-containing Staphylococcus aureus (mecC-MRSA) in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes targeting the genes for mecC-MRSA, along with kits are provided that are designed for the detection of mecC-MRSA.
    Type: Grant
    Filed: January 8, 2018
    Date of Patent: January 29, 2019
    Assignee: Roche Molecular Systems, Inc.
    Inventors: Jenny A. Johnson, Ashley Hayes
  • Patent number: 10189874
    Abstract: Novel compositions and methods for engineering wireframe architectures and scaffolds of increasing complexity by creating gridiron-like DNA structures (FIG. 1). A series of four-arm junctions are used as vertices within a network of double-helical DNA fragments. Deliberate distortion of the junctions from their most relaxed conformations ensures that a scaffold strand can traverse through individual vertices in multiple directions. DNA gridirons, ranging from two-dimensional arrays with reconfigurability to multilayer and three-dimensional structures and curved objects, can be assembled according the methods presented herein.
    Type: Grant
    Filed: February 25, 2015
    Date of Patent: January 29, 2019
    Assignee: Arizona Board of Regents on behalf of Arizona State University
    Inventors: Dongran Han, Hao Yan
  • Patent number: 10182987
    Abstract: A lipid membrane structure encapsulating an siRNA inside thereof and containing a lipid compound of the formula (I) as a lipid component (R1 and R2 represent CH3—(CH2)n—CH?CH—CH2—CH?CH—(CH2)m—, n represents an integer of 3 to 5, m represents an integer of 6 to 10, p represents an integer of 2 to 7, and R3 and R4 represent a C1-4 alkyl group or a C2-4 alkenyl group.
    Type: Grant
    Filed: May 18, 2015
    Date of Patent: January 22, 2019
    Assignee: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITY
    Inventors: Hideyoshi Harashima, Yusuke Sato, Shota Warashina, Hiroto Hatakeyama, Mamoru Hyodo, Takashi Nakamura
  • Patent number: 10184123
    Abstract: Therapies and assays to screen for small molecules that can have therapeutic use in the control of neurodegenerative diseases such as Parkinson's and other alpha-synucleinopathies.
    Type: Grant
    Filed: July 17, 2017
    Date of Patent: January 22, 2019
    Assignee: RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY
    Inventors: M. Maral Mouradian, Eunsung Junn
  • Patent number: 10179931
    Abstract: The present invention provides methods for immobilizing target nucleic acids on a solid support utilizing combinatorial capture probe pairs. These pairs contain first and second capture oligonucleotides that each comprise a target binding region, a capture region and a stem region positioned between the target binding and capture regions. The target binding regions comprise nucleic acid sequences that allow them to hybridize to adjacent regions on the target nucleic acid. The stem regions have nucleic acid sequences that are complementary to each other and the capture regions each comprise a sequence that when positioned adjacent to one another produce a combined nucleic acid sequence that is complementary to a portion of an oligonucleotide bound to a solid support.
    Type: Grant
    Filed: July 18, 2017
    Date of Patent: January 15, 2019
    Inventor: Lyle J. Arnold, Jr.
  • Patent number: 10174352
    Abstract: The present invention provides methods for amplifying a nucleic acid from a sample containing a mixture of nucleic acids utilizing a solid support. Methods are provided utilizing user-defined primer oligonucleotides for directional amplification that assists in further manipulation of the target nucleic acid, such as sequencing. Methods are also provided utilizing blocker and displacer oligonucleotides for generating amplified target nucleic acids of defined length. One of these methods provides a first oligonucleotide and a second oligonucleotide affixed to a solid support or separate solid supports. The first oligonucleotide is blocked to prevent extension from the 3?-terminus and has a sequence complementary to a first portion of a target nucleic acid. The second oligonucleotide has a sequence that is identical to a second portion of the target nucleic acid. In this method, a sample is applied to the solid support and the target nucleic acid within the sample binds said first oligonucleotide.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: January 8, 2019
    Inventors: Lyle J. Arnold, Norman C. Nelson
  • Patent number: 10174358
    Abstract: The present invention provides a novel assay that allows high-throughput screening of chemical compounds for the inhibition of binding between EF-Tu and tRNA.
    Type: Grant
    Filed: October 31, 2014
    Date of Patent: January 8, 2019
    Assignee: Rutgers, The State University of New Jersey
    Inventors: Wlodek Mandecki, Emanuel Goldman, Maxim Chudaev
  • Patent number: 10174310
    Abstract: The invention provides methods, compositions, kits and devices for the detection of target molecules. In some embodiments, the invention allows for multiplexed target molecule detection.
    Type: Grant
    Filed: August 8, 2013
    Date of Patent: January 8, 2019
    Assignee: ROCHE SEQUENCING SOLUTIONS, INC.
    Inventor: Garry P. Nolan
  • Patent number: 10176277
    Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample. In some embodiments, a dissociation curve correction can be calibrated for the sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.
    Type: Grant
    Filed: August 27, 2013
    Date of Patent: January 8, 2019
    Assignee: LIFE TECHNOLOGIES CORPORATION
    Inventors: Francis T. Cheng, Casey R. McFarland
  • Patent number: 10168335
    Abstract: The present invention relates to a method of biological labeling that occurs via a free radical chain reaction. The labeling occurs due to deposition of a detectable reporter molecule from a media comprising a substance comprising at least two moieties of a peroxidase enzyme substrate (termed herein ‘cross-linker’) in a target site comprising peroxidase activity and a biological marker. The labeling reaction described herein may generally be used to detect targets in a host of experimental schemes for detecting and visualizing a biological or chemical target, including immunohistochemistry (IHC), in situ hybridization (ISH), antibody-based staining methods such as ELISA, Southern, Northern, and Western blotting, and others.
    Type: Grant
    Filed: May 26, 2016
    Date of Patent: January 1, 2019
    Assignee: DAKO DENMARK A/S
    Inventors: Jesper Lohse, Kenneth Heesche Petersen
  • Patent number: 10167253
    Abstract: This invention includes ionizable compounds, and compositions and methods of use thereof. The ionizable compounds can be used for making nanoparticle compositions for use in biopharmaceuticals and therapeutics. More particularly, this invention relates to compounds, compositions and methods for providing nanoparticles to encapsulate active agents, such as nucleic acid agents, and to deliver and distribute the active agents to cells, tissues, organs, and subjects.
    Type: Grant
    Filed: June 23, 2016
    Date of Patent: January 1, 2019
    Assignee: Nitto Denko Corporation
    Inventors: Roger Adami, Hao Bai, John Gaudette, Bharat Majeti, Seiji Nukui, Kwok Yin Tsang, Hai Wang, Haiqing Yin, Wenbin Ying
  • Patent number: 10160966
    Abstract: A method of preparing a nucleic acid sample with target enrichment uses a reaction vessel (11), within which is added a chelating agent to a sample with heating to about 99° C. to provide a crude lysate. A PNA probe is provided at a concentration sufficient for binding and capture of discernible levels of target nucleic acid. The PNA probe may be attached to beads (26) which are initially embedded in a wax body (17) and are released during the heating so that they are free to move and come into contact with the PNA probe and target DNA. After binding has occurred, the beads are magnetically attracted back into a pocket (16) along with the wax (17), which is allowed to solidify before they are removed from the reaction vessel.
    Type: Grant
    Filed: December 10, 2014
    Date of Patent: December 25, 2018
    Assignee: ALTRATECH LIMITED
    Inventors: Brian O'Farrell, Timothy Cummins, Cian Desmond O'Sullivan, Jorge Álvarez-Vicente
  • Patent number: 10161002
    Abstract: Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses.
    Type: Grant
    Filed: December 13, 2017
    Date of Patent: December 25, 2018
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Jonas Korlach, Jeffrey Wegener
  • Patent number: 10161933
    Abstract: The invention provides methods of detecting an analyte by multi-stage mass spectrometry with improved S/N ratio. An analyte is labeled with a positively-charged mass tag to form a precursor ion that leads by anchimeric assistance to a greatly enhanced, analyte-characteristic first product ion that can, in turn, lead to a greatly enhanced, analyte-characteristic second product ion in a mass spectrometer. Either a three stage mass spectrometer (true MS3) or a two-stage mass spectrometer (MS2) operated in a pseudo MS3 mode can be used. The precursor ion is split via an anchimeric-assisted reaction to form a first product ion, which in turn can be fragmented to form the second product ion. The methods offer extreme ultrasensitivity, at the low amol level. The invention also provides anchimeric mass tags for use in the methods. A wide variety of previously undetectable analytes of biological or environmental origin can be detected and quantified.
    Type: Grant
    Filed: June 28, 2018
    Date of Patent: December 25, 2018
    Inventors: Roger Giese, Poguang Wang
  • Patent number: 10155942
    Abstract: Methods of barcoding nucleic acids, such as genomic DNA, are provided herein. In some embodiments, a fragment of genomic DNA may comprise a first and a second barcode.
    Type: Grant
    Filed: November 5, 2013
    Date of Patent: December 18, 2018
    Assignee: Takara Bio USA, Inc.
    Inventors: Takao Kurihara, Emmanuel Kamberov, Tim Tesmer, John Langmore
  • Patent number: 10154671
    Abstract: A composition and method for improving plant growth, wherein the composition includes at least one live strain of bacteria of the Lactobacillus rhamnosus species.
    Type: Grant
    Filed: October 30, 2013
    Date of Patent: December 18, 2018
    Assignee: DANSTAR FERMENT AG
    Inventors: Olivier Cor, Bruno Sanchez
  • Patent number: 10150872
    Abstract: Multimeric protected fluorescent reagents and their methods of synthesis are provided. The reagents are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The reagents contain fluorescent dye elements, that allow the compounds to be detected with high sensitivity at desirable wavelengths, binding elements, that allow the compounds to be recognized specifically by target biomolecules, and protective shield elements, that decrease undesirable contacts between the fluorescent dye elements and the bound target biomolecules and that therefore decrease photodamage of the bound target biomolecules by the fluorescent dye elements. The reagents also contain coupling elements connect monomeric compounds into multimeric forms, thereby increasing brightness.
    Type: Grant
    Filed: February 4, 2016
    Date of Patent: December 11, 2018
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Frank Zheng, Jeremiah Hanes, Gene Shen, Louis Brogley, Stephen Yue, Yuri Lapin, John Lyle, Honey Osuna, Andrei Fedorov, Lubomir Sebo
  • Patent number: 10144961
    Abstract: This invention provides novel azido linkers for deoxynucleotide analogs having a detectable marker attached thereto.
    Type: Grant
    Filed: May 16, 2017
    Date of Patent: December 4, 2018
    Assignee: THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK
    Inventors: Jingyue Ju, Huanyan Cao, Zengmin Li, Qinglin Meng, Jia Guo, Shenglong Zhang, Lin Yu
  • Patent number: 10138514
    Abstract: Some embodiments described herein relate to modified nucleotide and nucleoside molecules with novel 3?-hydroxy protecting groups. Also provided herein are methods to prepare such modified nucleotide and nucleoside molecules and sequencing by synthesis processes using such modified nucleotide and nucleoside molecules.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: November 27, 2018
    Assignee: Illumina Cambridge Limited
    Inventors: Xiaohai Liu, Xiaolin Wu, Geoffrey Paul Smith
  • Patent number: 10138458
    Abstract: A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: November 27, 2018
    Assignee: Akonni Biosystems, Inc.
    Inventors: Phillip Belgrader, Benjamin Hindson
  • Patent number: 10131936
    Abstract: According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: November 20, 2018
    Assignee: Life Technologies Corporation
    Inventors: Scott C. Benson, Cinna Monighetti, Sandy M. Koepf
  • Patent number: 10131890
    Abstract: Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: November 20, 2018
    Assignee: TAKARA BIO INC.
    Inventors: Kiyoyuki Matsumura, Nariaki Takatsu, Takashi Uemori, Hiroyuki Mukai
  • Patent number: 10131902
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: November 20, 2018
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Carol Kreader
  • Patent number: 10127348
    Abstract: Systems and methods are used to display data obtained from a qPCR instrument. Each of two or more samples is probed with a first labeling probe and a second labeling probe. A first data set is received from a qPCR instrument at a first cycle number that includes for each sample a first labeling probe intensity, and a second labeling probe intensity. A second data set is received at a second cycle number that includes for each sample a first labeling probe intensity and a second labeling probe intensity. A first plot of first labeling probe intensity as a function of second labeling probe intensity is created using the first data set. A second plot of first labeling probe intensity as a function of second labeling probe intensity is created using the second data set. The first plot and the second plot are displayed in response to user defined input to provide dynamic and real-time analysis of genotyping data.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: November 13, 2018
    Assignee: Life Technologies Corporation
    Inventors: Gordon A. Janaway, Evelyn Wing-Sim Chan
  • Patent number: 10125367
    Abstract: The present invention relates to a pharmaceutical composition for preventing or treating atopic dermatitis, the pharmaceutical composition including, as an active ingredient, X-shaped DNA (XL-DNA) formed by complementary binding of oligonucleotides having nucleotide sequences of SEQ ID NO: 1 to SEQ ID NO: 4. When the pharmaceutical composition is subcutaneously injected into an animal model of atopic dermatitis, effects of easing skin lesions, such as erythema, bleeding and edema, and the like, and ear edema, and reducing expression of immunoglobulin E (IgE) are exhibited. In this regard, the composition can be used as a pharmaceutical composition, a health food, or a cosmetic for atopic dermatitis.
    Type: Grant
    Filed: April 6, 2016
    Date of Patent: November 13, 2018
    Assignee: THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION
    Inventors: Joo Young Lee, Gabsik Yang
  • Patent number: 10125364
    Abstract: The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ALAS1 gene, and methods of using such dsRNA compositions to alter (e.g., inhibit) expression of ALAS1.
    Type: Grant
    Filed: July 31, 2015
    Date of Patent: November 13, 2018
    Assignees: ALYNYLAM PHARMACEUTICALS, INC., ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
    Inventors: Brian Bettencourt, Kevin Fitzgerald, William Querbes, Robert J. Desnick, Makiko Yasuda
  • Patent number: 10119163
    Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.
    Type: Grant
    Filed: March 2, 2016
    Date of Patent: November 6, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
  • Patent number: 10119162
    Abstract: Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5? or 3? flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5? and 3? flaps are generated. The flaps are cleaved using 5? or 3? flap endonucleases or 3? to 5? exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.
    Type: Grant
    Filed: November 16, 2016
    Date of Patent: November 6, 2018
    Assignee: AFFYMETRIX, INC.
    Inventors: Jianbiao Zheng, Li Weng, Malek Faham
  • Patent number: 10119143
    Abstract: The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ALAS1 gene, and methods of using such dsRNA compositions to alter (e.g., inhibit) expression of ALAS1.
    Type: Grant
    Filed: October 3, 2014
    Date of Patent: November 6, 2018
    Assignees: ALNYLAM PHARMACEUTICALS, INC., ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
    Inventors: Brian Bettencourt, Kevin Fitzgerald, William Querbes, Robert J. Desnick, Makiko Yasuda
  • Patent number: 10113172
    Abstract: The present invention relates to optimized aptamers and methods of using these aptamers.
    Type: Grant
    Filed: April 17, 2017
    Date of Patent: October 30, 2018
    Assignee: University of Iowa Research Foundation
    Inventors: Paloma Giangrande, Francis Miller, William Thiel
  • Patent number: 10115034
    Abstract: Systems and methods generate a projected image at an optimal exposure time. Images are captured different exposure times. Pixels that satisfy an intensity threshold percentage for each image are selected. The intensity values of the selected pixels are then evaluated to determine whether the selected pixels are distributed above a lower intensity threshold and below an upper intensity threshold. The linear relationship is projected to determine an optimal exposure time that has an optimal exposure time duration that exceeds each exposure time duration associated with each of the captured images when the linear relationship exists between each of the captured images. A projected image associated with the optimal exposure time is generated from one or more of the captured images.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: October 30, 2018
    Assignees: LIFE TECHNOLOGIES HOLDINGS PTE LIMITED, PIERCE BIOTECHNOLOGY, INC.
    Inventors: Suk Hong, Nikki Jarrett, Eric Hommema, Jason Aravich, Yanpeng Cao, Benyong Shi, Kok Siong Teo
  • Patent number: 10116490
    Abstract: A method for use by an apparatus of a communication network comprises detecting (S22) that a user or user equipment attaching to the communication network belongs to a usage group, based on group identity information that is allocated to the user or user equipment and identifies the usage group, detecting (S24), based on the group identity information, whether or not a first rules function out of several rules functions has been allocated to another user or user equipment of the usage group, and in case it is detected that the first rules function has been allocated to another user or user equipment of the usage group, selecting (S26), for the user or user equipment, the first rules function.
    Type: Grant
    Filed: September 7, 2012
    Date of Patent: October 30, 2018
    Assignee: NOKIA SOLUTIONS AND NETWORKS OY
    Inventor: Juha Antero Rasanen
  • Patent number: 10113203
    Abstract: The present invention relates to a kit and nucleic acid chip for diagnosing bladder cancer using a bladder cancer-specific marker gene. More particularly, the invention relates to a kit and nucleic acid chip for diagnosing bladder cancer, which can detect the promoter methylation of a bladder cancer-specific gene, the promoter or exon region of which is methylated specifically in transformed cells of bladder cancer. The use of the diagnostic kit or nucleic acid chip of the invention enables diagnosis of bladder cancer at an early stage of transformation, thus enabling early diagnosis of bladder cancer, and can diagnose bladder cancer in a more accurate and rapid manner compared to a conventional method.
    Type: Grant
    Filed: September 19, 2017
    Date of Patent: October 30, 2018
    Assignee: GENOMICTREE, INC.
    Inventors: Sung Whan An, Young Ho Moon, Tae Jeong Oh
  • Patent number: 10106838
    Abstract: Composition, method and kit of parts for a simplified and safe extraction of nucleic acids from biological samples, such as crude and processed food, and subsequent analysis by polymerase chain reaction to the presence of animal material, genetically modified organisms, allergens and pathogens. The method comprises the addition of extracting composition together with hot water for extraction and stabilization of nucleic acids as well as removal of substances interfering with DNA polymerase activity.
    Type: Grant
    Filed: March 3, 2015
    Date of Patent: October 23, 2018
    Assignee: IFP PRIVATES INSTITUT FÜR PRODUKTQUALITÄT GMBH
    Inventors: Wolfgang Weber, Christine Werner
  • Patent number: 10106850
    Abstract: The invention relates to a method for the high throughput discovery, detection and genotyping of one or more genetic markers in one or more samples, comprising the steps of restriction endonuclease digest of DNA, adaptor-ligation, optional pre-amplification, selective amplification, pooling of the amplified products, sequencing the libraries with sufficient redundancy, clustering followed by identification of the genetic markers within the library and/or between libraries and determination of (co-)dominant genotypes of the genetic markers.
    Type: Grant
    Filed: August 22, 2017
    Date of Patent: October 23, 2018
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, Anker Preben Sørensen, Marco Gerardus Maria Van Schriek
  • Patent number: 10105674
    Abstract: The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: October 23, 2018
    Assignee: Genomics USA, Inc.
    Inventor: Michael E. Hogan
  • Patent number: 10100365
    Abstract: The invention disclosed herein is based on the identification of novel mutations in the JAK2 gene and JAK2 protein. The invention provides compositions and methods useful for diagnosing hematopoietic diseases including, for example, myeloproliferative diseases. The invention also provides compositions and methods useful for determining a prognosis of an individual diagnosed as having a hematopoietic disease.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: October 16, 2018
    Assignee: Quest Diagnostics Investments Incorporated
    Inventors: Maher Albitar, Wanlong Ma
  • Patent number: 10100291
    Abstract: Methods of making deletion mutants of Cas9 proteins and making chimeric Cas9 proteins are described. The Cas9 N- and C-terminal domains may play critical roles in crRNAitracrRNA binding and/or PAM selectivity. To analyze activity, a series of domain exchange mutants between NM and STI (Streptococcus thermophilus Cas9) were made, replacing the N and/or C terminus of NM (Neisseria meningitides Cas9) with the homologous region from STI. The chimeric proteins were then tested using the transcriptional reporter assay described herein altering the guideRNA and/or Cas9 specific PAM within the reporter to determine the influence of the domain exchanges on protein specificity. None of the N-terminal domain swaps between NM and STI endowed NM with novel properties. The C-terminal exchange generated a NM-STI hybrid that was capable of interacting with the STI crRNAitracrRNA complex and was further able to suppress a reporter with a STI specific PAM.
    Type: Grant
    Filed: November 19, 2014
    Date of Patent: October 16, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Alejandro Chavez, Frank Poelwijk, George M. Church
  • Patent number: 10100349
    Abstract: Methods and compositions for determining the presence of a polymorphism at a target nucleotide position in a plurality of target nucleic acid sequences is provided.
    Type: Grant
    Filed: September 30, 2014
    Date of Patent: October 16, 2018
    Assignee: President and Fellows of Harvard College
    Inventors: Iain J. Macleod, Christopher F. Rowley, Max Essex
  • Patent number: 10093962
    Abstract: The present invention relates to compositions and methods of target enrichment or selection of nucleic acids using hybridization, which can be used in, e.g., next-generation sequencing.
    Type: Grant
    Filed: September 29, 2015
    Date of Patent: October 9, 2018
    Assignee: Agilent Technologies, Inc.
    Inventor: Michael Borns