With Significant Amplification Step (e.g., Polymerase Chain Reaction (pcr), Etc.) Patents (Class 435/6.12)
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Patent number: 10870863Abstract: The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use.Type: GrantFiled: December 4, 2018Date of Patent: December 22, 2020Assignee: Monsanto Technology LLCInventors: Ian W. Davis, Aabid Shariff
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Patent number: 10870675Abstract: The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance.Type: GrantFiled: January 9, 2018Date of Patent: December 22, 2020Assignee: SEEGENE, INC.Inventor: Jong Yoon Chun
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Patent number: 10871482Abstract: Provided are methods for detecting a target molecule or particle suspected to be present in a sample, comprising (a) contacting the sample with (i) a fusion molecule comprising a ligand capable of binding to the target molecule or particle and a binding domain, and (ii) a polymer scaffold comprising at least one binding motif to which the binding domain is capable of binding, under conditions that allow the target molecule or particle to bind to the ligand and the binding domain to bind to the binding motif; (b) loading the polymer into a device comprising a pore that separates an interior space of the device into two volumes, and configuring the device to pass the polymer through the pore from one volume to the other volume, wherein the device further comprises a sensor adjacent to the pore configured to identify objects passing through the pore; and (c) determining, with the sensor, whether the fusion molecule or particle bound to the binding motif is bound to the target molecule or particle, thereby detectType: GrantFiled: May 20, 2016Date of Patent: December 22, 2020Assignee: Ontera Inc.Inventors: Trevor J. Morin, Daniel Alexander Heller, William B. Dunbar
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Patent number: 10870893Abstract: The present invention relates to methods and compositions for determination of and uses of specific methylation patterns indicative of adenoma and carcinoma. In particular, the invention relates to analysis of defined CpG loci that are coordinately methylated in DNAs from cancer and adenoma samples, methods for identifying coordinately methylated loci, and methods of using analysis of coordinately methylated loci in one or more marker regions in the design of assays for adenoma and cancer.Type: GrantFiled: October 28, 2019Date of Patent: December 22, 2020Inventors: David A. Ahlquist, William R. Taylor, Hongzhi Zou, Graham P. Lidgard
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Patent number: 10865409Abstract: The present invention relates to oligonucleotide-encoded libraries and methods of tagging such libraries. In particular, the methods and oligonucleotides can include one or more 2?-substituted nucleotides, such as 2?-O-methyl or 2?-fluoro nucleotides, and other conditions or reagents to enhance enzyme ligation or one or more chemical functionalities to support chemical ligation.Type: GrantFiled: September 7, 2012Date of Patent: December 15, 2020Assignee: X-Chem, Inc.Inventors: Anthony D. Keefe, Richard W. Wagner, Alexander Litovchick, Matthew Clark, John W. Cuozzo, Ying Zhang, Paolo A. Centrella, Christopher D. Hupp
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Patent number: 10865447Abstract: The present invention discloses methods, kits, and apparatus as well as reagents and compositions associated therewith for deriving an indicator for use in diagnosing the presence, absence or degree of at least one condition in a biological subject or in prognosing at least one condition in a biological subject. Also disclosed is a biomarker signature for use in diagnosing the presence, absence or degree of at least one condition in a biological subject or in prognosing at least one condition in a biological subject. The present invention further discloses methods, kits and apparatus, as well as reagents and compositions associated therewith, for identifying biomarkers for use in a biomarker signature.Type: GrantFiled: May 15, 2015Date of Patent: December 15, 2020Assignee: ImmuneXpress Pty LtdInventors: Richard Bruce Brandon, Leo Charles McHugh
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Patent number: 10858646Abstract: A method of separating a target double-stranded nucleic acid molecule from a sample including the target double-stranded nucleic acid molecule and a non-target double-stranded nucleic acid molecule, including (1) mixing the sample, a pyrrole-imidazole-containing polyamide (first PI polyamide) modified with a first linker molecule and capable of specifically binding to a sequence of the target double-stranded nucleic acid molecule, and a carrier a modified with a first ligand capable of specifically binding and/or adsorbing to the first linker molecule such that a mixed solution is produced, (2) forming a complex A by binding the carrier a to the first PI polyamide with which the target double-stranded nucleic acid molecule is bound in the mixed solution, and (3) separating the complex A from the mixed solution.Type: GrantFiled: April 9, 2018Date of Patent: December 8, 2020Assignee: TOPPAN PRINTING CO., LTD.Inventors: Hiroki Nagase, Kei Tsukamoto, Shiro Kitano
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Patent number: 10858692Abstract: Compositions and methods for making a plurality of probes for analyzing a plurality of nucleic acid samples are provided. Compositions and methods for analyzing a plurality of nucleic acid samples to obtain sequence information in each nucleic acid sample are also provided.Type: GrantFiled: April 4, 2018Date of Patent: December 8, 2020Inventors: George M. Church, Kun Zhang, Joseph Chou
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Patent number: 10858694Abstract: The present invention provides a method of amplifying an RNA molecule in a biological sample by reverse transcription PCR (RT-PCR), wherein the RT-PCR is carried out in a solution comprising a) a polar aprotic solvent; b) a serum albumin; and optionally c) a non-ionic surfactant and/or a betaine.Type: GrantFiled: December 18, 2015Date of Patent: December 8, 2020Assignee: Global Life Sciences Solutions Operations UK LtdInventors: Alexander Yarunin, Peter James Tatnell, Kathryn Louise Lamerton, Rebecca Fullerton
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Patent number: 10851406Abstract: The invention is in general directed to the rapid exponential amplification of short DNA or RNA sequences at a constant temperature.Type: GrantFiled: May 22, 2017Date of Patent: December 1, 2020Assignee: Ionian Technologies, LLCInventors: Brian K Maples, Rebecca C. Holmberg, Andrew P. Miller, Jarrod Provins, Richard Roth, Jeffrey Mandell
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Patent number: 10851131Abstract: Provided is a method including detecting an incorporation of a labelled nucleotide into a nascent polynucleotide strand complementary to a template polynucleotide strand by a polymerase, wherein the polymerase is tethered to a solid support conductive channel by a tether and the labelled nucleotides is a compound of Formula I:Type: GrantFiled: February 19, 2019Date of Patent: December 1, 2020Assignees: ILLUMINA, INC., ILLUMINA SINGAPORE PTE. LTD.Inventors: Jeffrey Mandell, Silvia Gravina, Sergio Peisajovich, Kaitlin Pugliese, Yin Nah Teo, Xiangyuan Yang, Maria Candelaria Rogert Bacigalupo
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Patent number: 10851402Abstract: The present invention relates to a method for in vitro detection and/or monitoring of a disease in a sample, based on measurement of enzymatic activity of proteases activated and secreted upon disease development, to modified peptides used for the enzymatic detection of the proteases, the use of the peptides, a kit comprising such peptides and the use of ADAM-protease activity as a surrogate marker for disease burden and activity in infectious, inflammatory, and malignant diseases, such as HIV infection and melanoma.Type: GrantFiled: January 9, 2014Date of Patent: December 1, 2020Assignee: Friedrich-Alexander-Universitaet Erlangen-NuernbergInventors: Andreas Baur, Kalle Saksela, Gerold Schuler
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Patent number: 10844441Abstract: Disclosed herein are methods and compositions for human identification using polymorphisms in the Penta E short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.Type: GrantFiled: May 16, 2016Date of Patent: November 24, 2020Assignee: LIFE TECHNOLOGIES CORPORATIONInventor: Chang Zhong
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Patent number: 10845333Abstract: An object is to make a bridge of DNA expanding between a pair of electrodes, and to characterize the bridge of DNA, to thereby detect DNA easily and surely without employing any marker or labeling substances, such as fluorescent reagents. A method of detecting DNA using a detection device with at least a couple of electrodes, the method comprising immobilizing a primer on the electrodes; making a bridge of the DNA expanded between the electrodes, by immersing the electrodes in a solution including circular templates of single stranded DNA, annealing the circular templates, and generating single stranded DNA product utilizing RCA (Rolling Circle Amplification), with impressing a designated voltage between the electrodes; and characterizing the bridge of DNA which includes multiple single stranded DNA molecules between the electrodes.Type: GrantFiled: October 24, 2014Date of Patent: November 24, 2020Assignees: The Foundation for the Promotion of Industrial Science, Neuroindx, Inc., Centre National de la Recherche Scientifique (CNRS)Inventors: Hiroyuki Fujita, Stanislav L. Karsten, Dominique Collard, Momoko Kumemura
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Patent number: 10844369Abstract: Disclosed is a process for isolating cell-free nucleic acid (including both DNA and RNA) or an analog thereof from a bodily fluid, and which entails: a) mixing in a container the bodily fluid, a chaotropic agent in solid form, a detergent and a buffer, and a solid phase which includes magnetic particles, thus forming a reaction mixture containing the cell-free nucleic acid; b) magnetically separating the solid phase having the cell-free nucleic acid bound thereto from the reaction mixture; and optionally c) dissociating the nucleic acid from the solid phase. Compositions and kits are also disclosed.Type: GrantFiled: March 2, 2020Date of Patent: November 24, 2020Inventor: Nancy Quan
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Patent number: 10837051Abstract: A method for producing a dry reagent composition includes preparing a reagent solution including a Good's buffer in a concentration of more than 2.5 mM, an ammonium salt, a drying protection agent, and a nucleic acid amplification enzyme, and drying the reagent solution. A method for suppressing a decrease in enzyme activity during drying includes adding a Good's buffer in a biochemical reagent comprising an ammonium salt and an enzyme prior to drying the enzyme.Type: GrantFiled: November 8, 2017Date of Patent: November 17, 2020Assignee: KANEKA CORPORATIONInventors: Tatsuya Toga, Shigehiko Miyamoto, Hozumi Tanaka
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Patent number: 10829810Abstract: The disclosure provides solid compositions such as lyophilisates adhered to surfaces such as plasma-treated surfaces and related methods, uses, kits, intermediates, starting materials, and downstream products.Type: GrantFiled: September 29, 2017Date of Patent: November 10, 2020Assignee: Gen-Probe IncorporatedInventors: Byron J. Knight, David Opalsky
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Patent number: 10829813Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.Type: GrantFiled: June 14, 2018Date of Patent: November 10, 2020Assignee: Boreal Genomics, Inc.Inventors: Milenko Despotovic, Joel Pel, Andrea Marziali
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Patent number: 10822659Abstract: Arrays for genome-wide analysis of methylation are disclosed. In a preferred aspect arrays comprising a plurality of probes complementary to a plurality of identified CpG islands in the human, mouse and rat genome are disclosed. The arrays may be used to detect methylation within CpG islands in samples from human, mouse and rat genomes.Type: GrantFiled: October 24, 2017Date of Patent: November 3, 2020Assignee: AFFYMETRIX, INC.Inventors: Yanxiang Cao, Shivani Nautiyal, Charles G. Miyada, Christopher Davies, Gangwu Mei, Alan J. Williams, Eric B. Schell, John E. Blume
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Patent number: 10825553Abstract: The disclosure provides methods to assemble genomes of eukaryotic or prokaryotic organisms. The disclosure further provides methods for haplotype phasing and meta-genomics assemblies.Type: GrantFiled: February 20, 2018Date of Patent: November 3, 2020Assignee: The Regents of the University of CaliforniaInventors: Richard E. Green, Jr., Liana F. Lareau
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Patent number: 10822647Abstract: Method to perform a PCR assay that comprises the following steps: a. Obtaining a nucleic acid sample; b. Hybridizing that nucleic acid sample to one or more pair of primers where at least one primer consists of a single stranded DNA polynucleotide having a length of 60 or more nucleotides; c Subjecting said nucleic acid sample to a PCR, wherein the reaction mixture medium contains at least one of said primers; and d. Detecting the length of the amplified products. The amplified nucleic acid may contain any sequence or multiple sequences of STRs (short tandem repeats), genes or any coding region having a defined location on a genome. The preferred nucleic acid samples to be amplified are degraded or fragmented and contain one or more genetic markers.Type: GrantFiled: October 3, 2016Date of Patent: November 3, 2020Assignee: BIODYNAMICS S.R.L.Inventor: Martin Eduardo Mautner
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Patent number: 10822638Abstract: Provided herein is technology relating to the amplification-based detection of bisulfite-treated DNAs and particularly, but not exclusively, to methods and compositions for multiplex amplification of low-level sample DNA prior to further characterization of the sample DNA. The technology further provides methods for isolating DNA from blood or blood product samples, e.g., plasma samples.Type: GrantFiled: October 26, 2016Date of Patent: November 3, 2020Assignee: Exact Sciences Development Company, LLCInventors: Hatim Allawi, Graham P. Lidgard, Brian Aizenstein, Tamara J. Sander, Maria Giakoumopoulos, Michael W. Kaiser, Melissa M. Gray, Abram Michael Vaccaro
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Patent number: 10815475Abstract: A method of extracting DNA and/or RNA from a cell or capsid, the method comprising steps of: (a) contacting a composition comprising the cell or capsid with a composition comprising (ii) a quaternary ammonium compound or a precursor thereof; and (b) contacting the composition obtained in step (a) with a composition comprising a proteinaceous washing agent.Type: GrantFiled: July 28, 2016Date of Patent: October 27, 2020Assignee: ARCIS BIOTECHNOLOGY HOLDINGS LIMITEDInventors: Jan Rogers, Carlos Toro Rueda
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Patent number: 10815237Abstract: The present disclosure relates generally to the field of antimicrobial compounds and to methods of making and using them. These compounds are useful for treating, preventing, reducing the risk of, and delaying the onset of microbial infections in humans and animals. In some embodiments, the present disclosure provides a compound of Formula (I) or a tautomer thereof or a pharmaceutically acceptable salt of the compound or tautomer.Type: GrantFiled: May 5, 2017Date of Patent: October 27, 2020Assignee: BIOVERSYS AGInventors: Erin M. Duffy, Ashoke Bhattacharjee, Zoltan F. Kanyo, Joseph A. Ippolito
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Patent number: 10808288Abstract: This invention is related to novel PNA probes, probe sets, methods and kits pertaining to the detection of one or more species of Candida yeast. Non-limiting examples of probing nucleobase sequences that can be used for the probes of this invention can be selected from the group consisting of: AGA-GAG-CAG-CAT-GCA (Seq. Id. No. 1), AGA-GAG-CAA-CAT-GCA (Seq. Id. No. 2), ACA-GCA-GAA-GCC-GTG (Seq. Id. No. 3), CAT-AAA-TGG-CTA-CCA-GA (Seq. Id. No. 4), CAT-AAA-TGG-CTA-CCC-AG (Seq. Id. No. 5), ACT-TGG-AGT-CGA-TAG (Seq. Id. No. 6), CCA-AGG-CTT-ATA-CTC-GC (Seq. Id. No. 7), CCC-CTG-AAT-CGG-GAT (Seq. Id. No. 8), GAC-GCC-AAA-GAC-GCC (Seq. Id. No. 9), ATC-GTC-AGA-GGC-TAT-AA (Seq. Id. No. 10), TAG-CCA-GAA-GAA-AGG (Seq. Id. No. 11), CAT-AAA-TGG-CTA-GCC-AG (Seq. Id. No. 12), CTC-CGA-TGT-GAC-TGC-G (Seq. Id. No. 13), TCC-CAG-ACT-GCT-CGG (Seq. Id. No. 14), TCC-AAG-AGG-TCG-AGA (Seq. Id. No. 15), GCC-AAG-CCA-CAA-GGA (Seq. Id. No. 16), GCC-GCC-AAG-CCA-CA (Seq. Id. No. 17), GGA-CTT-GGG-GTT-AG (Seq. Id. No.Type: GrantFiled: August 28, 2017Date of Patent: October 20, 2020Assignee: APPLIED BIOSYSTEMS, LLCInventors: Jens J. Hyldig-Nielsen, Henrik Stender, Kenneth M. Oliveira, Susan Rigby
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Patent number: 10809163Abstract: A device for collecting biological material, characterized in that it comprises a rod having, at one end, a dry absorbent body having a surface area of between approximately 1 and approximately 3.14 mm2, said absorbent body comprising surfactants and denaturing agents. The present invention also relates to the use of said device for sampling biological material from a biological trace having any surface area greater than a micro surface area of around 1 mm2 or having any volume greater than a micro-volume of around 1 microliter, and to a method for collecting biological material and a method for collecting and analyzing and/or identifying biological material, comprising a step of putting said device in contact with said biological material.Type: GrantFiled: February 17, 2016Date of Patent: October 20, 2020Assignee: L'ETAT FRANCAIS REPRESENTE PAR LE MINISTERE DE L'INTERIEURInventor: Sylvain Hubac
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Patent number: 10808278Abstract: Kits and methods for detecting pathogens without the need for laboratory equipment are disclosed. The kits and methods described herein allow for near-room temperature amplification of pathogen polynucleotides in a biological sample in a one-compartment reaction vessel. The kits and methods may be used to detect any target nucleic acid, such as DNA or RNA from a bacterial, fungal, or viral pathogen.Type: GrantFiled: March 2, 2016Date of Patent: October 20, 2020Assignee: UNIVERSITY OF MIAMIInventors: Sylvia Daunert, Sapna Deo, Erin Kobetz, David Broyles, Anita Manfredi
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Patent number: 10801056Abstract: The present invention relates to a kit for detecting miRNA and a method for detecting miRNA using the kit. According to the miRNA detection kit and method of the present invention, it is possible to detect a certain miRNA in a quick and accurate manner, and it also possible to perform multiplex analysis capable of detecting a plurality of miRNAs at the same time.Type: GrantFiled: August 8, 2017Date of Patent: October 13, 2020Assignee: HEIMBIOTEK INC.Inventor: Jae Hoon Lee
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Patent number: 10799501Abstract: Provided herein is a pharmaceutical combination comprising (a) a poly-(ADP-ribose)-polymerase (PARP) inhibitor and (b) a second agent comprising (i) an inhibitor of glycogen synthase kinase 3 (GSK-3) or (ii) an inhibitor of disrupter of telomeric silencing 1-like (DOT1L). Also provided is a method of treating a subject suffering from acute myeloid leukaemia, comprising administering to the subject a therapeutically effective amount of the pharmaceutical combination.Type: GrantFiled: November 4, 2016Date of Patent: October 13, 2020Assignee: King's College Hospital NHS Foundation TrustInventor: Chi Wai Eric So
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Patent number: 10793854Abstract: Provided are a gene involved in the regulation of the amount of melanin in keratinocytes, and the control of the color of skin or hair, the gene being selected from the group consisting of ATG7 gene, RAB11A gene, CLIP-170 gene, Rubicon gene and RAB7B gene, and a molecule encoded by the gene.Type: GrantFiled: November 21, 2016Date of Patent: October 6, 2020Assignee: Kao CorporationInventors: Daiki Murase, Akira Hachiya
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Patent number: 10793851Abstract: Compositions and methods for tracking combinations of mutations in populations by coupling DNA assembly and paired-end sequencing technology with high-throughput sequencing. This allows for an unlimited number of mutation sites in a population of microorganisms to be identified, and allows sites to be sequenced across several replicates. Unique identifiers (DNA barcodes) can be used to measure fitness data by sequencing of the barcodes multiplexed with the mutation sites with high-throughput short sequencing read technology, allowing selections to be rapidly performed on populations of known combinatorial genotypes.Type: GrantFiled: August 4, 2016Date of Patent: October 6, 2020Assignee: The Regents of the University of Colorado, a body corporateInventors: Ryan T. Gill, Ramsey Zeitoun
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Patent number: 10796000Abstract: A sequence mining platform (SMP) comprises a processor, at least one machine-accessible storage medium responsive to the processor, and a sequence manager in the machine-accessible storage medium. The sequence manager is configured to use processing resources to determine a sequence of nucleobases in a nucleic acid. The storage medium also comprises a blockchain manager to (a) collect transaction data for one or more transactions for a blockchain which requires a proof of work (POW) for each new block; and (b) include at least some of the transaction data in a new block for the blockchain. The storage medium also comprises a sequence mining module (SMM) to use the determined sequence of nucleobases from the sequence manager to create a POW for the new block. In one embodiment, the SMM enables an entity which controls the SMP to receive transaction rewards and sequencing rewards. Other embodiments are described and claimed.Type: GrantFiled: June 11, 2016Date of Patent: October 6, 2020Assignee: Intel CorporationInventors: Ned M. Smith, Rajesh Poornachandran
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Patent number: 10796783Abstract: A method and system for selection of a primer set for a set of target sequences includes: identifying candidate fragments upon performance of a comparison operation with the set of target sequences; identifying a forward subset of unique forward candidates and a reverse subset of unique reverse candidates; reducing the forward subset and the reverse subset based on a filtering operation; performing an in silico search operation with the reduced forward subset and the reduced reverse subset; generating a set of candidate degenerate primers based on the in silico search operation and a base mismatch criterion; generating a reduced set of candidate degenerate primers upon filtering the set of candidate degenerate primers according to the set of primer criteria; and selecting forward primer subsets that amplify the set of target sequences and identifying a reverse primer subset for each of the forward primer subsets with an in silico PCR operation.Type: GrantFiled: August 18, 2016Date of Patent: October 6, 2020Assignee: PSOMAGEN, INC.Inventors: Daniel Almonacid, Zachary Apte, Jessica Richman
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Patent number: 10793888Abstract: The present invention provides a method for scarless in vitro DNA assembly using thermostable exonucleases and ligase, which relates to the field of genetic engineering. The present invention provides a fast method for assembling DNA subfragments with homologous ends, which employs thermostable polymerases and ligase in a thermal cycle of denaturation, annealing, digestion and ligation. After denaturation, DNA subfragments are assembled together via annealing of the homologous end sequences, the unpaired single-stranded overhangs are digested by polymerases, and the resulting nicked gaps are sealed by a ligase. Using this method, 2-6 DNA subfragments were successfully assembled within two hours. This method can be used in conventional DNA recombination and be adapted to high throughput assembly operations. In addition, combinatorial mutations can be easily introduced into the assembled sequence by use of primers with mutated bases.Type: GrantFiled: November 9, 2016Date of Patent: October 6, 2020Assignee: Jiangnan UniversityInventors: Zhen Kang, Jian Chen, Peng Jin, Guocheng Du, Wenwen Ding
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Patent number: 10788452Abstract: A method is provided for analyzing a sample including a population of biological entities using at least one microfabricated device. A plurality of the microwells on the microfabricated device are each uniquely indexed, and loaded with a sample such that at least some microwells each include more than one cell of a biological entity. The microfabricated device was incubated at predetermined conditions, and a selected genetic material of the cells of the biological entities obtained from the incubation is amplified to obtaining amplicons. An aggregate of the amplicons are sequenced obtain sequencing data, based on which and the indexing of the microwells, an identification of the biological entities present in each of the plurality of microwells is obtained. Such identification can then be used to determine a relationship between different types of biological entities in the sample.Type: GrantFiled: September 28, 2017Date of Patent: September 29, 2020Assignee: GENERAL AUTOMATION LAB TECHNOLOGIES INC.Inventors: Peter Christey, Alexander Hallock
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Patent number: 10787651Abstract: A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.Type: GrantFiled: April 13, 2018Date of Patent: September 29, 2020Assignees: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD., EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGYInventors: Huilei Yu, Yan Zhang, Jianhe Xu, Qian Zhao, Jiang Pan, Feng Liu, Guoqiang Lin
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Patent number: 10787694Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.Type: GrantFiled: May 11, 2018Date of Patent: September 29, 2020Assignee: Bio-Rad Laboratories, Inc.Inventors: Xiao-Song Gong, John Sullivan, Rongdian Fu, Yan Wang, Evan H. Bursey, Man Cheng
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Patent number: 10787676Abstract: This invention relates to the identification of a regulator protein (termed CYP78A6, or EOD3) which controls the size of plant seeds and organs in Arabidopsis and other plants. Manipulation of CYP78A protein expression may useful, for example, in improving crop yield and increasing plant biomass.Type: GrantFiled: June 20, 2017Date of Patent: September 29, 2020Assignee: Institute of Genetics and Development Biology Chinese Academy of SciencesInventors: Yunhai Li, Wenjuan Fang, Zhibiao Wang, Rongfeng Cui
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Patent number: 10781476Abstract: This disclosure provides, among other things, a method for processing a membrane comprising rolling circle amplification (RCA) products. In some embodiments, this method may comprise: (a) obtaining a porous capillary membrane that comprises fluorescently labeled RCA products that are in or on the membrane; (b) depositing a curable polymer onto the membrane; and (c) curing the curable polymer to encapsulate the RCA products in a solid. In some embodiments, the curable polymer may be a silicone and may be transparent in its solid form. A kit for performing the method and a composition made by the method are also provided.Type: GrantFiled: October 4, 2017Date of Patent: September 22, 2020Assignee: VANADIS DIAGNOSTICSInventors: Mathias Howell, Ove Öhman, Fredrik Persson, Linus Olausson
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Patent number: 10781478Abstract: Systems, methods and compositions of matter according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are systems, methods and compositions concerning the enhancement of nucleic acid hybridization specificity and controlling the shapes of melting curves revealed by nucleic acid hybrid pairs to optimize nucleic acid analysis. These systems, methods and compositions comprise producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch.Type: GrantFiled: February 15, 2019Date of Patent: September 22, 2020Inventor: Patricia Okura
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Patent number: 10780107Abstract: This invention provides an agent and a composition that induces cancer cell death and suppresses cancer cell proliferation. The agent and the composition comprises, as an active ingredient, a drug for suppressing GST-? and MRPL17 or comprises, as active ingredients, a drug for suppressing GST-? and a drug for suppressing MRPL17.Type: GrantFiled: June 23, 2017Date of Patent: September 22, 2020Assignee: NITTO DENKO CORPORATIONInventors: Hiroyuki Tanaka, Kenjirou Minomi
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Patent number: 10774377Abstract: This disclosure generally relates to improved methods, compositions, and systems for detecting, quantifying, and characterizing genomic sequences from heterogeneous genomic samples. The disclosed methods include providing a unique molecular identifier to the initial target sequences prior to amplification and addition of indexing barcodes. The methods, compositions, and systems can be employed to accurately detect and quantify operational taxonomic units from heterogeneous samples and further to detect sequencing errors, e.g., chimeric sequences, which may occur during the development and sequencing of the library.Type: GrantFiled: October 5, 2017Date of Patent: September 15, 2020Assignee: VERILY LIFE SCIENCES LLCInventors: Vanessa Ridaura, Jerrod George Schwartz
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Patent number: 10768173Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.Type: GrantFiled: September 23, 2019Date of Patent: September 8, 2020Assignee: ELEMENT BIOSCIENCES, INC.Inventors: Sinan Arslan, Molly He, Matthew Kellinger, Jake Levieux, Michael Previte, Junhua Zhao, Su Zhang
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Patent number: 10767221Abstract: Presented herein are polymerase-linked nucleotides for improved distinguishing nucleotide sequences for different nucleic acid molecules. Also presented are methods and systems using the polymerase-linked nucleotides for improved distinguishing nucleotide sequences for different nucleic acid molecules.Type: GrantFiled: March 15, 2013Date of Patent: September 8, 2020Assignee: Illumina, Inc.Inventors: Christian Gloeckner, Matthew William Kellinger, Lea Pickering
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Patent number: 10760065Abstract: Various aspects and embodiments of the invention are directed to methods and compositions for reversing antibiotic resistance or virulence in and/or destroying pathogenic microbial cells such as, for example, pathogenic bacterial cells. The methods include exposing microbial cells to a delivery vehicle with at least one nucleic acid encoding an engineered autonomously distributed circuit that contains a programmable nuclease targeted to one or multiple genes of interest.Type: GrantFiled: September 3, 2014Date of Patent: September 1, 2020Assignee: Massachusetts Institute of TechnologyInventors: Timothy Kuan-Ta Lu, Robert James Citorik, Mark Kyle Mimee
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Patent number: 10760120Abstract: The present disclosure provides methods and kits for performing high multiplex PCR using molecular barcodes. The methods disclosed herein separately extend a set of primers (BC primers) that each comprise a target-specific sequence, a molecular barcode and a universal sequence, and amplify the resulting extension products using another set of primers (LA primers) that each comprise another target-specific sequence and a universal sequence. The methods may further comprise amplification using universal primers (preferably comprising an adapter).Type: GrantFiled: January 21, 2016Date of Patent: September 1, 2020Assignee: QIAGEN SCIENCES, LLCInventors: Yexun Wang, Quan Peng
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Patent number: 10760116Abstract: The present invention provides a method for analyzing a template nucleic acid, a method for analyzing a target substance, an analysis kit for a template nucleic acid or a target substance, and an analyzer for a template nucleic acid or a target substance, which are excellent in accuracy.Type: GrantFiled: August 26, 2016Date of Patent: September 1, 2020Assignee: KABUSHIKI KAISHA DNAFORMInventors: Yuji Tanaka, Yoshihide Hayashizaki, Koichiro Tsujimaru
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Patent number: 10760064Abstract: Methods and constructs for RNA-guided targeting of transcriptional activators to specific genomic loci.Type: GrantFiled: March 14, 2014Date of Patent: September 1, 2020Assignee: The General Hospital CorporationInventors: J. Keith Joung, Morgan Maeder
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Patent number: 10760119Abstract: Disclosed are devices, systems and methods of use utilizing interfacial effects enabling droplet actuation, inhibition and early sensing of molecular reactions, such as of polymerase chain reaction.Type: GrantFiled: October 23, 2015Date of Patent: September 1, 2020Assignee: Arizona Board of Regents on behalf of the University of ArizonaInventors: Jeong-Yeol Yoon, Dustin Harshman
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Patent number: 10762982Abstract: A system and method for the detection of pathogens and other microbes using nucleotide analysis is described. Aligned and unaligned nucleotide sequences are utilized to predict the presence or absence of pathogens and other microbes.Type: GrantFiled: October 7, 2016Date of Patent: September 1, 2020Assignee: TRACE GENOMICS, INC.Inventors: Diane Wu, Poornima Parameswaran