Involving Bacterium, Fungus, Parasite Or Protozoan (e.g., Detecting Pathogen Virulence Factors, Adhesions, Toxins, Etc.) Patents (Class 435/6.15)
  • Patent number: 10392670
    Abstract: Compositions, reactions mixtures, kits, and systems for detecting bacterial contamination are provided, as well as methods of using the same.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: August 27, 2019
    Inventors: James Jian Quan Wang, Xiangmin Cui, HiuNam Chan
  • Patent number: 10345304
    Abstract: The presently disclosed fluidic sensor system and method comprise multifunctional nanoprobe-enabled capture for early detection of chemical and/or biological pathogens in a liquid sample. This sensor system and method can be used for food and environmental monitoring.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: July 9, 2019
    Assignee: The Johns Hopkins University
    Inventors: Chao Wang, Kellogg Schwab, Charles Young
  • Patent number: 10304039
    Abstract: A product disassembling method and non-transitory computer readable media thereof are provided. According to the components of the product and the disassembling attributes of the components, the disassembling sequence that minimizes the total disassembling time can be planned. The method includes the following steps: an initial disassembling sequence solution is randomly generated. The disassembling sequence solution is updated by using an update mechanism. The disassembling time of the update disassembling sequence solution is calculated by using a fitness function. The best fitness value is obtained after the continuously updating, so as to determine whether to update or maintain the initial disassembling sequence solution. The corresponding disassembling sequence is considered as the best planning result.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: May 28, 2019
    Inventor: Wei-Chang Yeh
  • Patent number: 10294515
    Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes, and detecting the fusion probe after nuclease treatment. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes, and detecting these probes after nuclease treatment. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
    Type: Grant
    Filed: December 7, 2011
    Date of Patent: May 21, 2019
    Assignee: HTG Molecular Diagnostics, Inc.
    Inventors: Bruce Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
  • Patent number: 10273525
    Abstract: Provided herein are methods for detecting and identifying strains of mycobacteria, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided for the detection and differentiation of mycobacteria based on, for example, pathogenicity, species, and antibiotic resistance or sensitivity. Compositions and methods are provided herein to identify and differentiate mycobacteria in mixtures of different mycobacteria and mycobacteria and non-mycobacteria.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: April 30, 2019
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, John Rice
  • Patent number: 10266879
    Abstract: This invention provides compositions, methods, and systems for characterizing, resolving, and quantitating single stranded and double stranded DNA and RNA in-situ. Paired sense and anti-sense probes can signal the presence of double stranded nucleic acids. DNA and RNA can be distinguished in cell and tissue samples by hybridizing with probe sets adapted to highlight differences in these targets in-situ.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: April 23, 2019
    Inventors: Quan Nguyen, Yunqing Ma, Audrey Lin, Shauna Levinson, Chunfai Lai
  • Patent number: 10240211
    Abstract: The invention includes methods for obtaining samples of maternal tissue from seeds, obtaining genetic material from the maternal seed tissue, and performing a molecular analysis on the genetic material from the maternal seed tissue to determine maternal lineage of a single seed. The invention also includes methods for establishing a consensus maternal genotype from maternal seed tissues obtained from multiple seeds as well as methods for determining paternal lineage.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: March 26, 2019
    Inventors: Justin Schares, Yue Yun, Chengfeng Zhao
  • Patent number: 10191040
    Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: January 29, 2019
    Inventors: Chin-Yih Hong, Herng-Er Horng
  • Patent number: 10190176
    Abstract: This invention pertains to probes, primers and associated methods suitable for the analysis and diagnosis of Myco-bacterium tuberculosis, among other things.
    Type: Grant
    Filed: December 4, 2013
    Date of Patent: January 29, 2019
    Inventors: Guillermo E. Madico, Edward C. Jones Lopez
  • Patent number: 10138525
    Abstract: The disclosed invention include nucleic acid oligomers that may be used as amplification oligomers, including primers, as capture probes for sample preparation, and detection probes for detection of 16S rRNA from Atopobium vaginae in samples by using methods of specific nucleic acid amplification and detection.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: November 27, 2018
    Inventor: Damon K. Getman
  • Patent number: 10125386
    Abstract: An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: November 13, 2018
    Assignee: GeneWeave Biosciences, Inc.
    Inventors: Nikol De Forest, Werner Frei, Diego Rey, Shaunak Roy, Soni Shukla, Ryan C. Griswold, Kenneth G. Olson, Bruce J. Richardson, Victor H. Yee
  • Patent number: 10106578
    Abstract: Melanocortin receptor-specific linear peptides of the formula Z-Xaa1-Xaa2-Xaa3-Xaa4-Xaa5-Xaa6-Y??(VI) or a pharmaceutically acceptable salt thereof, where Z, Y, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, and Xaa6 are as defined in the specification, compositions and formulations including peptides of the foregoing formula or salts thereof, and pharmaceutical compositions for preventing, ameliorating or treating melanocortin-1 receptor-mediated or responsive diseases, indications, conditions and syndromes.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: October 23, 2018
    Assignee: Palatin Technologies, Inc.
    Inventors: Wei Yang, Yi-Qun Shi
  • Patent number: 10100353
    Abstract: The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3? part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5? part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3? part that is substantially homolog to a fourth part of the target sequence and a 5? part that is substantially complementary to a third part of the target sequence.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: October 16, 2018
    Inventor: Peter Warthoe
  • Patent number: 10101315
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: October 16, 2018
    Inventors: Amit Meller, Meni Wanunu
  • Patent number: 10053744
    Abstract: The present invention discloses a yeast strain with high yield of glutathione, and belongs to the technical field of microorganisms. Saccharomyces cerevisiae tlj2016 provided in the present invention has been deposited in China General Microbiological Culture Collection Center of Microbe Preservation Management Committee, with a preservation number of CGMCC No. 12789. The strain can tolerate glucose with tolerance capability reaching 300 g/L, and has tolerance capability against L-cysteine that is far higher than that of the starting strain, contributing to the production of glutathione at conditions of high concentrations of glucose, with a final concentration of GSH produced through fermentation in a 5-L fermentation tank reaching 3308 mg/L.
    Type: Grant
    Filed: December 26, 2016
    Date of Patent: August 21, 2018
    Assignee: Ningxia RisingMark Intellectual Property Consulting Co., Ltd.
    Inventors: Ji Li, Bingdian Li
  • Patent number: 9970064
    Abstract: A method of diagnosing bacterial vaginosis in a woman, which involves determining an amount of each of more than one BV-associated bacterium in a vaginal sample obtained from the female and assessing a BV status of the female based on the amount of each of the more than one BV-associated bacterium in the sample.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: May 15, 2018
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Charles Paul Cartwright, Bryndon Denae Lembke, Kalpana Ramachandran
  • Patent number: 9951392
    Abstract: Methods and kits are provided for calibrating a cell-free protein synthesis reaction for optimal activity. The method includes the steps of providing an extract competent for cell-free protein synthesis (CFPS); performing cell-free protein synthesis with the extract; measuring a first reaction end-point where in vitro protein synthesis plateaus; measuring a second reaction end-point where Energy Charge of the extract declines to a level in a range from about 0.40 to about 0.80 of Energy Charge of a control extract; and adjusting Energy Charge of the extract to a level in a range from about 0.80 to about 1.0 of the control extract.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: April 24, 2018
    Assignee: Northwestern University
    Inventors: Michael C. Jewett, Jennifer A. Schoborg, Charles Eric Hodgman, Mark J. Anderson
  • Patent number: 9932625
    Abstract: The invention provides a method for detection of mutant-type DNA or/and wild-type DNA by contacting at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) contacting the obtained mutant-type hybrid or/and wild-type hybrid with an intercalator, and (3) detecting the presence or absence of the mutant-type DNA or/and the wild-type DNA by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator.
    Type: Grant
    Filed: March 22, 2013
    Date of Patent: April 3, 2018
    Inventors: Shoichi Matsukuma, Tomokazu Ishikawa, Tatsuo Kurosawa
  • Patent number: 9884097
    Abstract: The present disclosure provides immunogenic materials and methods useful for reducing the risk of fungal infections, particularly valley fever. The disclosure also provides assays for identifying compounds useful to treat valley fever, as well as methods for ameliorating the symptoms of valley fever.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: February 6, 2018
    Inventors: Marc J. Orbach, Lisa F. Shubitz, Hema P. Narra
  • Patent number: 9868959
    Abstract: Described herein are engineered microalgae that exhibit enhanced lipid production during exponential growth. Such engineered microalgae are useful, for example, for the production of biofuels.
    Type: Grant
    Filed: December 13, 2011
    Date of Patent: January 16, 2018
    Assignee: J. Craig Venter Institute
    Inventors: Andrew Allen, Christopher Lee Dupont
  • Patent number: 9857303
    Abstract: The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, by compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsules; and identifying the compound which binds to or modulates the activity of the target. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: January 2, 2018
    Assignee: Medical Research Council
    Inventors: Andrew David Griffiths, Chris Abell, Florian Hollfelder, Enrico Mastrobattista
  • Patent number: 9850382
    Abstract: The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. The dyes of the invention possess superior fluorescent properties when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids, such as detection of nucleic acids in solution, in gels, in blots, in microarrays, and in bacteria and cells, and for use in analysis of cell structure, membrane integrity, and function.
    Type: Grant
    Filed: December 5, 2016
    Date of Patent: December 26, 2017
    Inventor: Laiqiang Ying
  • Patent number: 9851330
    Abstract: A nucleic acid (NA) detection method combines ultra-specific probe, on-chip isotachophoresis (ITP) which can separate single strand and double strand NAs, and enzyme amplification. The ITP device has a sieving matrix between the LE (leading electrolyte) and TE (trailing electrolyte) reservoirs, for separating double-strand and single-strand NAs. The LE or TE reservoir also contains a spacer ion having a mobility between the LE and the TE. The sample and a double-strand NA probe is added to the TE reservoir, the probe being formed of a protector strand modified with a fluorescent molecule and a complement strand, where the protector strand is released in the presence of the target NA. Fluorescent signal is detected downstream of the sieving matrix. Alternatively, the protector strand is modified with an enzyme and a single-strand NA modified with a substrate of the enzyme is added to the reaction mixture downstream of the sieving matrix.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: December 26, 2017
    Inventor: Noriaki Yamamoto
  • Patent number: 9822402
    Abstract: Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: November 21, 2017
    Inventors: Keith Thornton, Danielle Koffenberger, Paul Madepogu
  • Patent number: 9796760
    Abstract: The present invention discloses novel non-natural and modified peptides acting as cannabinoid (CB) receptors ligands, especially CB1 and/or CB2, useful as modulators of its activity; they are also described as a kit and a process in vitro for evaluating the binding to CB receptors, uses and pharmaceutical composition for modulating the CB receptors activity. The invention covers the non-natural peptide of SeqID:1 and those having at least 70% similarity related to the same, including as achievements specialty useful of the invention the non-natural peptides of SeqID:2, SeqID:3, SeqID:4, SeqID:5, SeqID:6.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: October 24, 2017
    Assignee: Proteimax Biotecnologia Ltda
    Inventor: Andrea Sterman Heimann
  • Patent number: 9778253
    Abstract: A bio-related substance assay tube 2 comprises a target substance capture bead 3 serving as a first microparticle, compensation bead 4 serving as a second microparticle on which a given amount of a bio-related substance has been immobilized, and a negative control bead 5 serving as a third microparticle for use as a negative control. A mount unit 12 comprises a nozzle communicating with a pump, and the bio-related substance assay tube 2 is mounted in the mount unit 12 to ensure communication with this nozzle. An analyte is introduced into the bio-related substance assay tube 2, followed by labeling the bio-related substance bound to each microparticle to cause light emission. Based on light emission from each microparticle, a calibration curve is prepared or the emission intensity of the first microparticle is compensated to quantify the bio-related substance.
    Type: Grant
    Filed: April 20, 2010
    Date of Patent: October 3, 2017
    Inventor: Hideji Tajima
  • Patent number: 9752184
    Abstract: The present invention provides methods for rapid forensic analysis of mitochondrial DNA and methods for characterizing heteroplasmy of mitochondrial DNA, which can be used to assess the progression of mitochondrial diseases.
    Type: Grant
    Filed: August 11, 2014
    Date of Patent: September 5, 2017
    Inventors: David J. Ecker, Richard H. Griffey, Rangarajan Sampath, Steven A. Hofstadler, John McNeil, Stanley T. Crooke, Lawrence B. Blyn, Thomas A. Hall, Yun Jiang, James C. Hannis, Neill K. White, Vivek Samant, Mark W. Eshoo, Jared J. Drader
  • Patent number: 9725758
    Abstract: A method of producing a replicate or derivative of an array of molecules, the array having a spatial arrangement of separate samples of molecules, includes creating, for each sample, at least one spatially limited effective area which is separate from the effective areas of the other samples, a surface, provided with a binding adapter or binding properties, of a carrier bordering on the effective areas. The molecules are amplified by means of amplifying agents in the effective areas for creating replicates or derivatives of the samples. The replicates or derivatives of the samples are bound to the carrier by means of the binding adapter or the binding properties, so that a spatial arrangement of the replicates or derivatives of the samples on the carrier corresponds to the spatial arrangement of the samples in the array. The carrier having the copies of the samples is removed from the array.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: August 8, 2017
    Inventors: Roland Zengerle, Felix Von Stetten, Guenter Roth, Jochen Hoffmann
  • Patent number: 9719142
    Abstract: Methods and kits for diagnosis, prognosis and treatment of metastatic tumors are provided where the metastatic tumor is characterized by changes in expression of +++, ++ and/or 11a variants of Mena.
    Type: Grant
    Filed: November 7, 2013
    Date of Patent: August 1, 2017
    Assignees: Albert Einstein College of Medicine, Inc., Massachusetts Institute of Technology, IFO-Regina Elena Cancer Institute
    Inventors: John S. Condeelis, Sumanta Goswami, Frank Gertler, Paola Nistico
  • Patent number: 9610337
    Abstract: The invention relates to the identification of antigens, including Mycobacterium sulphate assimilation pathway components such as CysD, for preventing and treating Mycobacterium infection, especially but not exclusively Mycobacterium tuberculosis infection; to expression systems including live Mycobacterium for expression of said antigens for prevention and treatment of said infection; and to use of said antigens and expression systems for prevention and treatment of said infection.
    Type: Grant
    Filed: December 20, 2012
    Date of Patent: April 4, 2017
    Assignee: The University of Sydney Centenary Institute of Cancer Medicine and Cell Biology
    Inventors: James Anthony Triccas, Rachel Pinto-Nadanachandran, Warwick John Britton
  • Patent number: 9598737
    Abstract: Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by semi-conductor sequencing, preferably ion-torrent sequencing. This method provides for full length analysis and of multiple areas (e.g. genes) of multiple genomes. These methods identify genetic mutations of a particular gene that are responsible for conferring resistance or sensitivity to an antibiotic or other chemical compound. Multiple different species, strains and/or serotypes of a particular organism are rapidly and efficiently screened and mutations identified along with the complete genome of an organism. By selecting primers pairs of similar size and GC content that produce amplicons with sequences spanning the entire genome, a single PCR reaction analyzed by ion torrent methodology can determine the sequence of a complete genome. Methods are useful to sequences the genomes of viral agents, such as influenza virus, and bacterial agents, such as tuberculosis bacteria.
    Type: Grant
    Filed: October 29, 2014
    Date of Patent: March 21, 2017
    Assignee: Longhorn Vaccines and Diagnostics, LLC
    Inventors: Luke T. Daum, Gerald W. Fischer
  • Patent number: 9574242
    Abstract: The invention relates to a method for detection of a methicillin resistant coagulase positive Staphylococcus aureus (MRSA) strain by means of a sequence specific amplification reaction.
    Type: Grant
    Filed: January 23, 2013
    Date of Patent: February 21, 2017
    Inventor: Steffen Mergemeier
  • Patent number: 9334542
    Abstract: Disclosed are compositions, assays, methods, diagnostic methods, kits and diagnostic kits for the specific and differential detection of a non-Mycobacterium avium subsp. paratuberculosis (non-MAP) organism, wherein a non-MAP organism is a Mycobacterium avium complex (MAC) organism that does not belong to the Mycobacterium avium subsp. paratuberculosis (MAP) organism, from samples including veterinary samples, clinical samples, food samples, forensic sample, an environmental sample (e.g., soil, dirt, garbage, sewage, air, or water), including food processing and manufacturing surfaces, or a biological sample. Exemplary non-MAP organisms including M. avium subsp. avium (MAA), M. avium subsp. hominissuis (MAH), and M. avium subsp. silvaticum (MAS) can be detected by the present compositions, kits and methods.
    Type: Grant
    Filed: March 5, 2013
    Date of Patent: May 10, 2016
    Inventors: Craig Cummings, Pius Brzoska, Angela Burrell, Catherine O'Connell
  • Patent number: 9207244
    Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. In some embodiments, the reader component communicates with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
    Type: Grant
    Filed: January 16, 2015
    Date of Patent: December 8, 2015
    Assignee: CUE INC.
    Inventors: Ayub Khattak, Clinton Sever
  • Patent number: 9181593
    Abstract: The disclosed invention include nucleic acid oligomers that may be used as amplification oligomers, including primers, as capture probes for sample preparation, and detection probes for detection of 16S rRNA from Atopobium vaginae in samples by using methods of specific nucleic acid amplification and detection.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: November 10, 2015
    Assignee: Gen-Probe Incorporated
    Inventor: Damon K. Getman
  • Patent number: 9133463
    Abstract: The present disclosure provides, inter alia, Thraustochytrium and relevant methods and reagents, including engineered regulatory sequences from and/or operative in Thraustochytrid or Thraustochytrium, selectable markers useful for engineering microorganisms such as Thraustochytrids, methods for mutagenizing microorganisms, novel strains produced by mutagenesis and methods and compositions related to production of particular compounds in microorganisms.
    Type: Grant
    Filed: March 7, 2012
    Date of Patent: September 15, 2015
    Inventors: ShuoCheng Zhang, Roberto Armenta
  • Patent number: 9119401
    Abstract: Provided are methods for identification of plant glutamine synthetase inhibitors as well as the plant synthetase inhibitors identified. Further provided are methods of modulating glutamine synthetase activity in a plant, particularly weeds by applying to the plant or substrate for cultivating the plant a microorganism (e.g. Burkholderia strain) in an amount effective to modulate glutamine synthetase activity.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: September 1, 2015
    Inventors: Huazhang Huang, Ratnakar Asolkar, Pamela Marrone
  • Patent number: 9090880
    Abstract: The invention relates to isolated polypeptides that include amino acid sequences within botryococcene synthase from different algal species. In another aspect, the invention relates to a method for increasing the production level of a botryococcene hydrocarbon molecule in a cell. The method includes increasing expression of a polynucleotide sequence that encodes botryococcene synthase in the cell. In a further aspect, the invention relates to an algal cell having a polynucleotide sequence that is genetically engineered to express a higher level of botryococcene synthase than a corresponding wild type algal cell, wherein the cell produces an increased level of a botryococcene hydrocarbon molecule than a corresponding wild type algal cell.
    Type: Grant
    Filed: June 24, 2009
    Date of Patent: July 28, 2015
    Assignee: Research Foundation of the City University of New York
    Inventors: Juergen Polle, Duc Tran
  • Patent number: 9051665
    Abstract: A method for screening biomolecules molecules is disclosed. One embodiment of the method includes inoculating an expression library or a portion of the expression library into an animal, monitoring the relative abundance of individual members of the expression library; and analyzing molecules expressed by members that show significantly reduced relative abundance after inoculation into the animal. Also disclosed is an expression vector and an expression library employing the vector.
    Type: Grant
    Filed: November 20, 2009
    Date of Patent: June 9, 2015
    Inventor: Steven L. Zeichner
  • Patent number: 9034642
    Abstract: The invention relates to the polynucleotide sequence of a nontypeable strain of Haemophilus influenzae (NTHi) and polypeptides encoded by the polynucleotides and uses thereof. The invention also relates to NTHi genes which are upregulated during or in response to NTHi infection of the middle ear and/or the nasopharynx.
    Type: Grant
    Filed: January 9, 2014
    Date of Patent: May 19, 2015
    Inventors: Lauren O. Bakaletz, Robert S. Munson, Jr., David W. Dyer
  • Publication number: 20150125867
    Abstract: The present disclosure relates generally to the field of nucleic acids and, more particularly, to aptamers capable of binding to toxins produced by Clostridium difficile; diagnostic kits and methods comprising such aptamers; and methods of making and using such aptamers.
    Type: Application
    Filed: September 29, 2014
    Publication date: May 7, 2015
    Inventors: Urs Ochsner, Evaldas Katilius, Nebojsa Janjic
  • Patent number: 9017956
    Abstract: Provided herein are uses of genes for HOG, Ras and cAMP signal transduction pathways to treat fungal infection. To regulate the HOG pathway of Cryptococcus neoformans, roles of SSK1, TCO2, SSK2, PBS2, HOG1, ENA1 and NHA1 genes were investigated to find that a biosynthesis level of ergosterol is increased when these genes are inhibited. When the genes are inhibited, a large amount of ergosterol is distributed on a fungal cell membrane. Accordingly, since there are many working points of an ergosterol-binding antifungal agent, an efficiency of the ergosterol-binding antifungal agent can be considerably improved. To regulate the Ras and cAMP pathways of Cryptococcus neoformans, roles of RAS1, RAS2, CDC24, GPA1, CAC1, ACA1, PKA1, HSP12 and HSP122 genes were investigated to find that a sensitivity to a polyene- or azole-based drug is increased when these genes are inhibited.
    Type: Grant
    Filed: April 15, 2013
    Date of Patent: April 28, 2015
    Assignee: Nutrex Technology Co., Ltd.
    Inventors: Yong-Sun Bahn, Young-Joon Ko, Shin-Ae Maeng, Kwang Woo Jung, Gyu Bum Kim
  • Publication number: 20150111216
    Abstract: Devices and methods for preparing and assessing a liquid sample are described which include: a microfluidic microscopy device including an absorbent structure including at least one dry dye for a liquid and configured to convey a polar liquid from a first end of the absorbent structure through the at least one dry dye for a liquid to a second end of the absorbent structure, a support structure positioned adjacent to a first surface of the absorbent structure, a lid structure positioned adjacent to a second surface of the absorbent structure, and a clamping structure positioned to maintain a relative position of the support structure and the lid structure to form a tapered internal chamber adjacent to the second end of the absorbent structure.
    Type: Application
    Filed: October 23, 2013
    Publication date: April 23, 2015
    Inventors: Charles Delahunt, David Gasperino, Matthew P. Horning, Kevin P. F. Nichols, Benjamin K. Wilson
  • Patent number: 9012146
    Abstract: The present invention relates to a method for selectively enriching and/or isolating microbial and optionally additionally viral nucleic acids from samples that contain a mixture of microbial cells, freely circulating nucleic acids and higher eukaryotic cells, and optionally additionally viruses, in a liquid, and to a kit for selectively enriching and/or isolating intracellular and extracellular microbial nucleic acids, and optionally additionally viral nucleic acids, from samples that contain a mixture of microbial and higher eukaryotic cells, freely circulating nucleic acids, in particular extracellular microbial nucleic acids, and optionally additionally viruses in a liquid.
    Type: Grant
    Filed: November 19, 2010
    Date of Patent: April 21, 2015
    Assignee: QIAGEN GmbH
    Inventors: Dirk Heckel, Thomas Doedt
  • Publication number: 20150104795
    Abstract: A method of increasing biogenic production of a combustible gas from a subterranean geologic formation is described. The method may include extracting formation water from the geologic formation, where the extracted formation water includes at least a first species and a second species of microorganism. The method may also include analyzing the extracted formation water to identify the first species of microorganism that promotes the biogenic production of the combustible gas. An amendment may be introduced to the formation water to promote the growth of the first species of microorganism, and the biological characteristics of the formation water may be altered to decrease a population of the second species in the geologic formation.
    Type: Application
    Filed: July 29, 2014
    Publication date: April 16, 2015
    Inventors: Robert S. Pfeiffer, Glenn Ulrich, Gary Vanzin, Verlin Dannar, Roland P. DeBruyn, James B. Dodson
  • Patent number: 8999649
    Abstract: The present invention provides a molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, and donor and acceptor fluorophore moieties, where the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being cleaved by the botulinum neurotoxin, and separates the donor and acceptor fluorophores by a distance of not more than 10 nm, and where emission spectrum of the donor fluorophore moiety overlaps with the excitation spectrum of the acceptor fluorophore moiety; or where the emission spectra of the fluorophores are detectably different. Also provided are isolated nucleic acid expressing the construct, kits comprising said construct and cell lines comprising said nucleic acid. Further provided are methods of detecting a BoNT using the above described construct via FRET, and methods for detecting a BoNT using surface plasmon resonance imaging.
    Type: Grant
    Filed: February 23, 2012
    Date of Patent: April 7, 2015
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: Edwin R. Chapman, Min Dong
  • Patent number: 9000137
    Abstract: The present invention provides nucleic acid aptamers that bind to Plasmodium proteins lactate dehydrogenase and histidine-rich protein II, and uses thereof for the diagnosis of malaria. Aptamers against histidine-rich protein II may be used to detect the presence of Plasmodium species in general, whereas aptamers against lactate dehydrogenase can be used to specifically detect Plasmodium falciparum.
    Type: Grant
    Filed: February 8, 2013
    Date of Patent: April 7, 2015
    Assignee: The University of Hong Kong
    Inventors: Julian A Tanner, Yee Wai Cheung, Masayo Kotaka
  • Publication number: 20150056627
    Abstract: A nucleic acid aptamer comprising the nucleotide sequence shown herein as SEQ ID No. 5, 6, 7, 8, 9, 10, 1, 2, 3 or 4 or a fragment thereof or a sequence which is at least 80% identical therewith and use of nucleic aptamers to detect the presence of pathogenic bacteria in a sample, particularly in a complex matrix—such as a food system.
    Type: Application
    Filed: October 31, 2012
    Publication date: February 26, 2015
    Inventors: Riikka Kärkkäinen, Mette Ryun Drasbek, Niall W.G. Young, Graham A. Bonwick, Christopher Smith
  • Publication number: 20150056624
    Abstract: The method for isolating nucleic acids from a food sample comprising the following steps: a) obtaining a food enrichment culture; b) transferring a portion of the food enrichment culture into a reaction vessel thereby providing a food enrichment sample and providing a water-immiscible phase in contact with the food enrichment culture; c) lysing the food enrichment sample to provide a lysed sample; d) isolating nucleic acids from the lysed sample. Food enrichment culture samples are known to contain high concentrations of organic and/or liposoluble inhibitors. By contacting the enrichment culture sample with a water-immiscible phase before the actual DNA extraction procedure starts, part of the lipophilic inhibitors is expected to cross the phase interface due to an enhanced solubility in the organic phase and thereby become depleted.
    Type: Application
    Filed: February 28, 2013
    Publication date: February 26, 2015
    Inventors: Janina Cramer, Sarah Fakih, Corinna Küppers, Holger Engel
  • Publication number: 20150056628
    Abstract: Methods for the detection and typing of bacterial strains from food products and dietary supplements, environmental samples, in vivo/in vitro samples, and for studying the natural diversity of the species are disclosed. Potential applications also include product development and/or detection and differentiation of new bacterial strains.
    Type: Application
    Filed: August 22, 2014
    Publication date: February 26, 2015
    Applicant: DANISCO A/S
    Inventors: W. Michael Russell, Rodolphe Barrangou, Phillippe Horvath