Involving Bacterium, Fungus, Parasite Or Protozoan (e.g., Detecting Pathogen Virulence Factors, Adhesions, Toxins, Etc.) Patents (Class 435/6.15)
  • Patent number: 11112411
    Abstract: Provided herein are methods for simultaneously detecting and quantifying antibodies which bind to the same target and have high sequence identity, such as eculizumab and ALXN1210, present together or alone in a biological sample.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: September 7, 2021
    Assignee: Alexion Pharmaceuticals, Inc.
    Inventors: Meng Chen, Ryan Pelto
  • Patent number: 11061032
    Abstract: The presently disclosed fluidic sensor system and method comprise multifunctional nanoprobe-enabled capture for early detection of chemical and/or biological pathogens in a liquid sample. This sensor system and method can be used for food and environmental monitoring.
    Type: Grant
    Filed: June 4, 2019
    Date of Patent: July 13, 2021
    Assignee: THE JOHNS HOPKINS UNIVERSITY
    Inventors: Chao Wang, Kellogg Schwab, Charles Young
  • Patent number: 11040330
    Abstract: Provided is a chromatography stationary phase having an excellent molecule discriminating ability. Specifically, provided is a chromatography stationary phase including a carrier carrying a copolymer that has a pyrrolidone backbone or a piperidone backbone, and an imide backbone in a repeating unit of the main chain.
    Type: Grant
    Filed: March 23, 2017
    Date of Patent: June 22, 2021
    Assignee: DAICEL CORPORATION
    Inventors: Toru Shibata, Satoshi Shinkura
  • Patent number: 11021534
    Abstract: The present invention provides an antigenic composition for use as a mycobacterial vaccine, said composition comprising (i) a first mycobacterial antigenic polypeptide, wherein said first mycobacterial antigenic polypeptide comprises a polypeptide sequence having at least 70% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 6, 12, 2, 18, 8, 10, 16, 4, or a fragment thereof having at least 50 consecutive amino acids thereof; or (ii) a first mycobacterial polynucleotide, wherein said first mycobacterial polynucleotide comprises a polynucleotide sequence encoding said first mycobacterial antigenic polypeptide, or wherein said first mycobacterial polynucleotide comprises a polynucleotide sequence selected from SEQ ID NO: 5, 11, 1, 17, 7, 9, 15, 3.
    Type: Grant
    Filed: August 8, 2017
    Date of Patent: June 1, 2021
    Assignee: SECRETARY OF STATE FOR HEALTH AND SOCIAL CARE
    Inventors: Yper Hall, Joanna Bacon, Philip Marsh
  • Patent number: 11015154
    Abstract: Disclosed herein are methods, compositions, and systems for determining specific microbial taxa, within a complex consortia of mixed taxa, which are interacting with each other in an environment of interest. In some embodiments, after diluting a sample comprising multiple different taxa of microorganisms, dilutions of the sample are cultivated for determining taxonomic information and interactions of multiple taxa of microorganisms in the sample.
    Type: Grant
    Filed: November 8, 2017
    Date of Patent: May 25, 2021
    Assignee: The Regents of the University of California
    Inventors: Nicholas B. Justice, Adam P. Arkin
  • Patent number: 11015228
    Abstract: The invention is directed to methods, kits, and compositions, for amplifying and detecting Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Trichomonas vaginalis (TV), and Mycoplasma genitalium (MG) in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.
    Type: Grant
    Filed: December 31, 2018
    Date of Patent: May 25, 2021
    Assignee: Abbott Molecular Inc.
    Inventor: Kevin Nelson
  • Patent number: 11001847
    Abstract: Compositions comprising aptamers capable of specifically binding to a surface protein of Clostridium difficile spore are provided. A method for detecting, enriching, separating, and/or isolating Clostridium difficile spores is provided.
    Type: Grant
    Filed: June 5, 2020
    Date of Patent: May 11, 2021
    Assignee: Biovector, Inc.
    Inventors: Ronald J. Shannon, Michael McIntyre, David Bunka, Edward Barnes
  • Patent number: 10988773
    Abstract: Described herein are engineered microalgae that exhibit enhanced lipid production during exponential growth. Such engineered microalgae are useful, for example, for the production of biofuels.
    Type: Grant
    Filed: January 16, 2018
    Date of Patent: April 27, 2021
    Assignee: J. Craig Venter Institute
    Inventors: Andrew Allen, Christopher L. Dupont
  • Patent number: 10948498
    Abstract: A cardiovascular event or disease is prevented or treated in a subject by measuring the expression level of soluble Triggering Receptors Expressed on Myeloid cells-1 (sTREM-1) level in a sample from the subject and comparing it to a reference level. Based on this comparison, subjects that are at risk of having or developing a cardiovascular event or disease are identified, and administered a suitable therapy.
    Type: Grant
    Filed: August 8, 2014
    Date of Patent: March 16, 2021
    Assignees: INOTREM, APHP (Assistance Publique-Hôpitaux de Paris), INSERM (Institut National de la Santé et de la Recherche Médicale), Université de Lorraine
    Inventors: Marc Derive, Sebastien Gibot, Hafid Ait-Oufella, Amir Boufenzer, Tabasomme Simon, Nicolas Danchin
  • Patent number: 10927386
    Abstract: The invention provides for spinach plants with broad-spectrum resistance to downy mildew disease and their progeny. Such plants may comprise unique combinations of alleles resulting in the broad-spectrum resistance to downy mildew. In certain aspects, compositions, including distinct polymorphic molecular markers, and methods for producing, using, identifying, selecting, and the like of plants or germplasm with resistance to downy mildew are provided.
    Type: Grant
    Filed: February 26, 2015
    Date of Patent: February 23, 2021
    Assignee: Seminis Vegetable Seeds, Inc.
    Inventors: Bart Willem Brugmans, John Meeuwsen, Claudia Nooyen
  • Patent number: 10874333
    Abstract: Systems and methods are presented for the diagnosis of middle ear pathological conditions based on spectral signatures. Preferred embodiments provide for detection of one or more analytes from the tympanic membrane. Devices use spectral measurements including spectral imaging to non-invasively identify middle ear pathological conditions including cholesteatoma and acute otitis media by providing real-time information of differentially expressed molecules. Devices and methods can also be used to non-invasively detect and quantify blood analytes from the tympanic membrane.
    Type: Grant
    Filed: September 15, 2016
    Date of Patent: December 29, 2020
    Assignees: Massachusetts Institute of Technology, Connecticut Children's Medical Center
    Inventors: Rishikesh Pandey, Nicolas Spegazzini, Luis H. Galindo, Ramachandra Dasari, Tulio Alberto Valdez
  • Patent number: 10829825
    Abstract: The present invention provides improved tests for the detection: of methicillin-resistant Staphylococcus aureus bearing a variant mecA gene. The tests are particularly useful for eliminating certain false negative results due to the presence of this variant in MRSA in patient samples.
    Type: Grant
    Filed: June 9, 2016
    Date of Patent: November 10, 2020
    Assignee: bioMerieux S.A.
    Inventors: Francois Paillier, Celine Chambon, Cathy Saint-Patrice
  • Patent number: 10822639
    Abstract: Provided herein is technology relating to isolating nucleic acids. In particular, the technology relates to methods and kits for extracting nucleic acids from problematic samples such as stool.
    Type: Grant
    Filed: December 6, 2018
    Date of Patent: November 3, 2020
    Assignee: Exact Sciences Corporation
    Inventors: Janelle J. Bruinsma, Michael J. Domanico, Graham P. Lidgard, Hongzhi Zou, William G. Weisburg, Hemanth D. Shenoi, James P. Light, II, Keith Kopitzke, John Zeis
  • Patent number: 10787715
    Abstract: Method and media for diagnosing Crohn's disease are provided. A method of diagnosing Crohn's disease in patients includes: obtaining a sample from an individual; culturing the sample to determine the presence or absence of Mycobacterium avium subspecies hominissuis (MAH) in the sample; and diagnosing the individual with Crohn's disease based on the determining the presence of MAH in the sample.
    Type: Grant
    Filed: November 21, 2017
    Date of Patent: September 29, 2020
    Assignee: PZM DIAGNOSTICS, LLC
    Inventors: Peilin Zhang, Lawrence M. Minardi
  • Patent number: 10760109
    Abstract: The present disclosure relates to methods for characterizing and diagnosing dental diseases. In particular, the present disclosure relates to methods for characterizing and diagnosing dental disease (e.g., gingivitis and periodontal disease) based on levels of AI-2 in oral fluids and plaque.
    Type: Grant
    Filed: June 8, 2015
    Date of Patent: September 1, 2020
    Assignees: THE REGENTS OF THE UNIVERSITY OF MICHIGAN, COLGATE-PALMOLIVE COMPANY
    Inventors: Alexander Rickard, William Giannobile, Michelle Swetky, Nielson T. Baxter, Steven E. Miller, Laurence Du-Thumm, Hans Stettler
  • Patent number: 10738327
    Abstract: The present invention relates to an electroporation device that may include many electroporation units and electroporation systems that can be used in an automated environment, e.g., as one station or module in a multi-station or multi-module cell processing environment. The electroporation device comprises an electroporation cuvette coupled with an adapter or engagement member at the top that is configured for engagement with liquid handling instrumentation, and a “sipper” conduit at the bottom for sample intake and output.
    Type: Grant
    Filed: August 22, 2018
    Date of Patent: August 11, 2020
    Assignee: Inscripta, Inc.
    Inventors: Jorge Bernate, Phil Belgrader, Don Masquelier, Vlorent Morina
  • Patent number: 10626466
    Abstract: The present invention provides compositions, methods and kits for the species-specific detection of Pseudomonas aeruginosa.
    Type: Grant
    Filed: December 23, 2014
    Date of Patent: April 21, 2020
    Assignee: GEN-PROBE INCORPORATED
    Inventors: Shannon K. Kaplan, Kristin Livezey, Jennifer J. Bungo, James J. Hogan
  • Patent number: 10603664
    Abstract: Devices, systems, and methods for detecting molecules of interest within a collected sample are described herein. In certain embodiments, self-contained sample analysis systems are disclosed, which include a reusable reader component, a disposable cartridge component, and a disposable sample collection component. The reader component may communicate with a remote computing device for the digital transmission of test protocols and test results. In various disclosed embodiments, the systems, components, and methods are configured to identify the presence, absence, and/or quantity of particular nucleic acids, proteins, or other analytes of interest, for example, in order to test for the presence of one or more pathogens or contaminants in a sample.
    Type: Grant
    Filed: April 25, 2019
    Date of Patent: March 31, 2020
    Assignee: CUE HEALTH INC.
    Inventors: Ayub Khattak, Clinton Sever, Paul Nelson, Ryan Cooper, Thomas Congdon, Justin Demartino, Raphael Shapiro, Mark Duncan
  • Patent number: 10597734
    Abstract: The application is related to novel signature sequences for diagnosis of Mycobacterium tuberculosis in clinical samples with 100% specificity and a very high degree of sensitivity.
    Type: Grant
    Filed: December 1, 2014
    Date of Patent: March 24, 2020
    Assignees: EUROPEAN MOLECULAR BIOLOGY LABORATORY, KUSUMA SCHOOL OF BIOLOGICAL SCIENCES INDIAN INSTITUTE OF TECHNOLOGY
    Inventors: Syed Asad Rahman, Javeed Ahmad, Nasreen Z Ehtesham, Seyed E Hasnain
  • Patent number: 10577610
    Abstract: The present disclosure relates to an EpCAM aptamer or an EpCAM aptamer conjugate having bi-functional activity and to uses thereof. In particular, the present disclosure also relates to an aptamer conjugate which binds to the transferrin receptor (TfR) and EpCAM which is useful for treatment of brain tumours and/or brain metastasis.
    Type: Grant
    Filed: February 11, 2016
    Date of Patent: March 3, 2020
    Assignee: Deakin University
    Inventor: Sarah Shigdar
  • Patent number: 10568317
    Abstract: The present invention relates to reduction of erythrocyte sedimentation rate in a blood sample. In particular, formulations, compositions, articles of manufacture, kits and methods for reduced erythrocyte sedimentation rate in a blood sample are provided.
    Type: Grant
    Filed: December 6, 2016
    Date of Patent: February 25, 2020
    Assignee: Biomatrica, Inc.
    Inventors: Joel Desharnais, Victoria Arendt, Margrith Mattmann
  • Patent number: 10501778
    Abstract: The present disclosure provides methods and devices for simultaneous identification of a plurality of target nucleic acid sequences in a single sample chamber that includes an addressable array of nucleic acid probes attached to a solid surface. Addressable signals can be generated and measured, in real-time, upon hybridization of target sequences at the individual probe locations within the array while the temperature of the system is varied. Such generated signals, as a function temperature, can then be used to compute the properties of nucleic acid hybridization at each addressable location which is ultimately utilized to estimate the sequence of the target nucleic acids. In particular, an integrated semiconductor biosensor array device can be used to measure the addressable signals.
    Type: Grant
    Filed: June 13, 2017
    Date of Patent: December 10, 2019
    Assignee: INSILIXA, INC.
    Inventors: Arjang Hassibi, Kshama Jirage, Arun Manickam, Kaveh Milaninia
  • Patent number: 10494679
    Abstract: The invention relates to a method for quantitative monitoring of bacterial endospores in an aqueous environment of a paper or board mill. The method comprises at least the following steps: obtaining at least a first aqueous sample originating from the industrial aqueous environment; destroying bacteria in vegetative form in the first sample by a suitable treatment, preferably by heating the first sample to a desired temperature; adding intercalating agent (such as PMA) to the treated first sample and allowing it to interact (e.g. by cross-linking) with the destroyed bacteria, so that the nucleic acid from the destroyed bacteria are unavailable for PCR; and determining the endospore level in the first sample by using quantitative polymerase chain reaction (qPCR) in which only the DNA from the endospores is available for amplification.
    Type: Grant
    Filed: February 26, 2016
    Date of Patent: December 3, 2019
    Assignee: Kemira Oyj
    Inventors: Kalle Riihinen, Marko Lauraeus, Marko Kolari, Juhana Ahola
  • Patent number: 10450617
    Abstract: A method of diagnosing bacterial vaginosis in a woman, which involves determining an amount of each of more than one BV-associated bacterium in a vaginal sample obtained from the female and assessing a BV status of the female based on the amount of each of the more than one BV-associated bacterium in the sample.
    Type: Grant
    Filed: May 14, 2018
    Date of Patent: October 22, 2019
    Assignee: Laboratory Corporation of America Holding
    Inventors: Charles Paul Cartwright, Bryndon Denae Lembke, Kalpana Ramachandran
  • Patent number: 10392670
    Abstract: Compositions, reactions mixtures, kits, and systems for detecting bacterial contamination are provided, as well as methods of using the same.
    Type: Grant
    Filed: July 23, 2014
    Date of Patent: August 27, 2019
    Assignee: DCH MOLECULAR DIAGNOSTICS, INC.
    Inventors: James Jian Quan Wang, Xiangmin Cui, HiuNam Chan
  • Patent number: 10345304
    Abstract: The presently disclosed fluidic sensor system and method comprise multifunctional nanoprobe-enabled capture for early detection of chemical and/or biological pathogens in a liquid sample. This sensor system and method can be used for food and environmental monitoring.
    Type: Grant
    Filed: November 24, 2015
    Date of Patent: July 9, 2019
    Assignee: The Johns Hopkins University
    Inventors: Chao Wang, Kellogg Schwab, Charles Young
  • Patent number: 10304039
    Abstract: A product disassembling method and non-transitory computer readable media thereof are provided. According to the components of the product and the disassembling attributes of the components, the disassembling sequence that minimizes the total disassembling time can be planned. The method includes the following steps: an initial disassembling sequence solution is randomly generated. The disassembling sequence solution is updated by using an update mechanism. The disassembling time of the update disassembling sequence solution is calculated by using a fitness function. The best fitness value is obtained after the continuously updating, so as to determine whether to update or maintain the initial disassembling sequence solution. The corresponding disassembling sequence is considered as the best planning result.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: May 28, 2019
    Assignee: NATIONAL TSING HUA UNIVERSITY
    Inventor: Wei-Chang Yeh
  • Patent number: 10294515
    Abstract: Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes, and detecting the fusion probe after nuclease treatment. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes, and detecting these probes after nuclease treatment. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.
    Type: Grant
    Filed: December 7, 2011
    Date of Patent: May 21, 2019
    Assignee: HTG Molecular Diagnostics, Inc.
    Inventors: Bruce Seligmann, BJ Kerns, John Luecke, Matt Rounseville, Ihab Botros, Mark Schwartz
  • Patent number: 10273525
    Abstract: Provided herein are methods for detecting and identifying strains of mycobacteria, and compositions and kits for performing such methods. In particular, nucleic acid amplification and fluorescence detection methods are provided for the detection and differentiation of mycobacteria based on, for example, pathogenicity, species, and antibiotic resistance or sensitivity. Compositions and methods are provided herein to identify and differentiate mycobacteria in mixtures of different mycobacteria and mycobacteria and non-mycobacteria.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: April 30, 2019
    Assignee: Brandeis University
    Inventors: Lawrence J. Wangh, John Rice
  • Patent number: 10266879
    Abstract: This invention provides compositions, methods, and systems for characterizing, resolving, and quantitating single stranded and double stranded DNA and RNA in-situ. Paired sense and anti-sense probes can signal the presence of double stranded nucleic acids. DNA and RNA can be distinguished in cell and tissue samples by hybridizing with probe sets adapted to highlight differences in these targets in-situ.
    Type: Grant
    Filed: January 20, 2016
    Date of Patent: April 23, 2019
    Inventors: Quan Nguyen, Yunqing Ma, Audrey Lin, Shauna Levinson, Chunfai Lai
  • Patent number: 10240211
    Abstract: The invention includes methods for obtaining samples of maternal tissue from seeds, obtaining genetic material from the maternal seed tissue, and performing a molecular analysis on the genetic material from the maternal seed tissue to determine maternal lineage of a single seed. The invention also includes methods for establishing a consensus maternal genotype from maternal seed tissues obtained from multiple seeds as well as methods for determining paternal lineage.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: March 26, 2019
    Assignee: PIONEER HI-BRED INTERNATIONAL, INC.
    Inventors: Justin Schares, Yue Yun, Chengfeng Zhao
  • Patent number: 10190176
    Abstract: This invention pertains to probes, primers and associated methods suitable for the analysis and diagnosis of Myco-bacterium tuberculosis, among other things.
    Type: Grant
    Filed: December 4, 2013
    Date of Patent: January 29, 2019
    Assignee: BOSTON MEDICAL CENTER CORPORATION
    Inventors: Guillermo E. Madico, Edward C. Jones Lopez
  • Patent number: 10191040
    Abstract: Multiplex immunoassays utilize the differential affinities among the conjugation pairs between the capture ligands and target analytes are proposed. Window magnetic-assisted rapid aptamer selection (window-MARAS) methods for selecting aptamers with desirable affinity toward the target analytes and methods for generating reagents for multiplex immunoassays or multiplex detection in one assay by utilizing the selected aptamers as capture ligands in reagents are described and used to demonstrate the feasibility of multiplex immunoassays based on the differential affinity of conjugation pairs between the capture ligands and target analytes.
    Type: Grant
    Filed: September 30, 2016
    Date of Patent: January 29, 2019
    Inventors: Chin-Yih Hong, Herng-Er Horng
  • Patent number: 10138525
    Abstract: The disclosed invention include nucleic acid oligomers that may be used as amplification oligomers, including primers, as capture probes for sample preparation, and detection probes for detection of 16S rRNA from Atopobium vaginae in samples by using methods of specific nucleic acid amplification and detection.
    Type: Grant
    Filed: October 28, 2015
    Date of Patent: November 27, 2018
    Assignee: GEN-PROBE INCORPORATED
    Inventor: Damon K. Getman
  • Patent number: 10125386
    Abstract: An apparatus includes a housing and an actuator. The housing, which defines a reagent volume that can receive a reagent container, can be removably coupled to a reaction chamber. The housing includes a puncturer that defines a transfer pathway in fluid communication with the reagent volume. A delivery portion of the housing defines a delivery pathway between the transfer pathway and the reaction chamber when the housing is coupled to the reaction chamber. The actuator has a plunger portion disposed within the reagent volume. An engagement portion of the actuator can be manipulated to move the plunger portion within the reagent volume to deform the reagent container. The puncturer can pierce a frangible portion of the reagent container to convey a reagent from the reagent container into the reaction chamber via the transfer pathway and/or the delivery pathway.
    Type: Grant
    Filed: November 30, 2016
    Date of Patent: November 13, 2018
    Assignee: GeneWeave Biosciences, Inc.
    Inventors: Nikol De Forest, Werner Frei, Diego Rey, Shaunak Roy, Soni Shukla, Ryan C. Griswold, Kenneth G. Olson, Bruce J. Richardson, Victor H. Yee
  • Patent number: 10106578
    Abstract: Melanocortin receptor-specific linear peptides of the formula Z-Xaa1-Xaa2-Xaa3-Xaa4-Xaa5-Xaa6-Y??(VI) or a pharmaceutically acceptable salt thereof, where Z, Y, Xaa1, Xaa2, Xaa3, Xaa4, Xaa5, and Xaa6 are as defined in the specification, compositions and formulations including peptides of the foregoing formula or salts thereof, and pharmaceutical compositions for preventing, ameliorating or treating melanocortin-1 receptor-mediated or responsive diseases, indications, conditions and syndromes.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: October 23, 2018
    Assignee: Palatin Technologies, Inc.
    Inventors: Wei Yang, Yi-Qun Shi
  • Patent number: 10100353
    Abstract: The present invention relates to a method and a kit of parts for detecting the presence or absence of one or more target nucleic acid sequences in a sample, the method comprising a sequence of steps for pre-amplifying the sample by means of a polymerase chain reaction, followed by a sequence of steps comprising an isothermal amplification of the pre-amplified sample, wherein the isothermal amplification comprises a pair of primers comprising a forward primer having a 3? part that is substantially complementary to a first part of the target sequence, the presence or absence of which is to be detected, and a 5? part that is substantially homolog to a second part of the target sequence, and a reverse primer comprising a 3? part that is substantially homolog to a fourth part of the target sequence and a 5? part that is substantially complementary to a third part of the target sequence.
    Type: Grant
    Filed: May 13, 2013
    Date of Patent: October 16, 2018
    Inventor: Peter Warthoe
  • Patent number: 10101315
    Abstract: Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Recently, electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. A new class of chemically modified nanopore sensors are provided with two approaches for monolayer coating of nanopores by: (1) self-assembly from solution, in which nanopores ?10 nm diameter can be reproductibly coated, and (2) self-assembly under voltage-driven electrolyte flow, in which 5 nm nanopores may be coated. Applications of chemically modified nanopore are provided including: the detection of biopolymers such as DNA and RNA; immobilizing enzymes or other proteins for detection or for generating chemical gradients; and localized pH sensing.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: October 16, 2018
    Assignee: TRUSTEES OF BOSTON UNIVERSITY
    Inventors: Amit Meller, Meni Wanunu
  • Patent number: 10053744
    Abstract: The present invention discloses a yeast strain with high yield of glutathione, and belongs to the technical field of microorganisms. Saccharomyces cerevisiae tlj2016 provided in the present invention has been deposited in China General Microbiological Culture Collection Center of Microbe Preservation Management Committee, with a preservation number of CGMCC No. 12789. The strain can tolerate glucose with tolerance capability reaching 300 g/L, and has tolerance capability against L-cysteine that is far higher than that of the starting strain, contributing to the production of glutathione at conditions of high concentrations of glucose, with a final concentration of GSH produced through fermentation in a 5-L fermentation tank reaching 3308 mg/L.
    Type: Grant
    Filed: December 26, 2016
    Date of Patent: August 21, 2018
    Assignee: Ningxia RisingMark Intellectual Property Consulting Co., Ltd.
    Inventors: Ji Li, Bingdian Li
  • Patent number: 9970064
    Abstract: A method of diagnosing bacterial vaginosis in a woman, which involves determining an amount of each of more than one BV-associated bacterium in a vaginal sample obtained from the female and assessing a BV status of the female based on the amount of each of the more than one BV-associated bacterium in the sample.
    Type: Grant
    Filed: May 9, 2017
    Date of Patent: May 15, 2018
    Assignee: Laboratory Corporation of America Holdings
    Inventors: Charles Paul Cartwright, Bryndon Denae Lembke, Kalpana Ramachandran
  • Patent number: 9951392
    Abstract: Methods and kits are provided for calibrating a cell-free protein synthesis reaction for optimal activity. The method includes the steps of providing an extract competent for cell-free protein synthesis (CFPS); performing cell-free protein synthesis with the extract; measuring a first reaction end-point where in vitro protein synthesis plateaus; measuring a second reaction end-point where Energy Charge of the extract declines to a level in a range from about 0.40 to about 0.80 of Energy Charge of a control extract; and adjusting Energy Charge of the extract to a level in a range from about 0.80 to about 1.0 of the control extract.
    Type: Grant
    Filed: March 16, 2015
    Date of Patent: April 24, 2018
    Assignee: Northwestern University
    Inventors: Michael C. Jewett, Jennifer A. Schoborg, Charles Eric Hodgman, Mark J. Anderson
  • Patent number: 9932625
    Abstract: The invention provides a method for detection of mutant-type DNA or/and wild-type DNA by contacting at least one of a single-stranded DNA having a substituted nucleotide, a deficient nucleotide region, or an inserted nucleotide region (mutant-type DNA), or/and a wild-type single-stranded DNA corresponding to the mutant-type DNA (wild-type DNA) with a probe hybridizing with both single-stranded DNAs, to form a hybrid with the mutant-type DNA (mutant-type hybrid) or/and a hybrid with the wild-type DNA (wild-type hybrid) (at least one of the obtained mutant-type hybrid and wild-type hybrid has a loop structure), (2) contacting the obtained mutant-type hybrid or/and wild-type hybrid with an intercalator, and (3) detecting the presence or absence of the mutant-type DNA or/and the wild-type DNA by separating the conjugate of mutant-type hybrid and intercalator or/and the conjugate of wild-type hybrid and intercalator.
    Type: Grant
    Filed: March 22, 2013
    Date of Patent: April 3, 2018
    Assignees: WAKO PURE CHEMICAL INDUSTRIES, LTD., KANAGAWA PREFECTURAL HOSPITAL ORGANIZATION
    Inventors: Shoichi Matsukuma, Tomokazu Ishikawa, Tatsuo Kurosawa
  • Patent number: 9884097
    Abstract: The present disclosure provides immunogenic materials and methods useful for reducing the risk of fungal infections, particularly valley fever. The disclosure also provides assays for identifying compounds useful to treat valley fever, as well as methods for ameliorating the symptoms of valley fever.
    Type: Grant
    Filed: March 11, 2014
    Date of Patent: February 6, 2018
    Assignee: THE ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA
    Inventors: Marc J. Orbach, Lisa F. Shubitz, Hema P. Narra
  • Patent number: 9868959
    Abstract: Described herein are engineered microalgae that exhibit enhanced lipid production during exponential growth. Such engineered microalgae are useful, for example, for the production of biofuels.
    Type: Grant
    Filed: December 13, 2011
    Date of Patent: January 16, 2018
    Assignee: J. Craig Venter Institute
    Inventors: Andrew Allen, Christopher Lee Dupont
  • Patent number: 9857303
    Abstract: The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, by compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsules; and identifying the compound which binds to or modulates the activity of the target. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.
    Type: Grant
    Filed: August 12, 2011
    Date of Patent: January 2, 2018
    Assignee: Medical Research Council
    Inventors: Andrew David Griffiths, Chris Abell, Florian Hollfelder, Enrico Mastrobattista
  • Patent number: 9851330
    Abstract: A nucleic acid (NA) detection method combines ultra-specific probe, on-chip isotachophoresis (ITP) which can separate single strand and double strand NAs, and enzyme amplification. The ITP device has a sieving matrix between the LE (leading electrolyte) and TE (trailing electrolyte) reservoirs, for separating double-strand and single-strand NAs. The LE or TE reservoir also contains a spacer ion having a mobility between the LE and the TE. The sample and a double-strand NA probe is added to the TE reservoir, the probe being formed of a protector strand modified with a fluorescent molecule and a complement strand, where the protector strand is released in the presence of the target NA. Fluorescent signal is detected downstream of the sieving matrix. Alternatively, the protector strand is modified with an enzyme and a single-strand NA modified with a substrate of the enzyme is added to the reaction mixture downstream of the sieving matrix.
    Type: Grant
    Filed: March 7, 2016
    Date of Patent: December 26, 2017
    Assignee: KONICA MINOLTA LABORATORY U.S.A., INC.
    Inventor: Noriaki Yamamoto
  • Patent number: 9850382
    Abstract: The invention describes the preparation and use of fluorescent stains for nucleic acids derived from unsymmetrical cyanine dyes. The dyes of the invention possess superior fluorescent properties when complexed with nucleic acids, and have utility in any application which requires detection of nucleic acids, such as detection of nucleic acids in solution, in gels, in blots, in microarrays, and in bacteria and cells, and for use in analysis of cell structure, membrane integrity, and function.
    Type: Grant
    Filed: December 5, 2016
    Date of Patent: December 26, 2017
    Inventor: Laiqiang Ying
  • Patent number: 9822402
    Abstract: Nucleic acids sequences that can be used for nucleic acid amplification, for example quantitative nucleic acid amplification, are provided herein.
    Type: Grant
    Filed: March 13, 2013
    Date of Patent: November 21, 2017
    Assignee: BECTON, DICKINSON AND COMPANY
    Inventors: Keith Thornton, Danielle Koffenberger, Paul Madepogu
  • Patent number: 9796760
    Abstract: The present invention discloses novel non-natural and modified peptides acting as cannabinoid (CB) receptors ligands, especially CB1 and/or CB2, useful as modulators of its activity; they are also described as a kit and a process in vitro for evaluating the binding to CB receptors, uses and pharmaceutical composition for modulating the CB receptors activity. The invention covers the non-natural peptide of SeqID:1 and those having at least 70% similarity related to the same, including as achievements specialty useful of the invention the non-natural peptides of SeqID:2, SeqID:3, SeqID:4, SeqID:5, SeqID:6.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: October 24, 2017
    Assignee: Proteimax Biotecnologia Ltda
    Inventor: Andrea Sterman Heimann
  • Patent number: 9778253
    Abstract: A bio-related substance assay tube 2 comprises a target substance capture bead 3 serving as a first microparticle, compensation bead 4 serving as a second microparticle on which a given amount of a bio-related substance has been immobilized, and a negative control bead 5 serving as a third microparticle for use as a negative control. A mount unit 12 comprises a nozzle communicating with a pump, and the bio-related substance assay tube 2 is mounted in the mount unit 12 to ensure communication with this nozzle. An analyte is introduced into the bio-related substance assay tube 2, followed by labeling the bio-related substance bound to each microparticle to cause light emission. Based on light emission from each microparticle, a calibration curve is prepared or the emission intensity of the first microparticle is compensated to quantify the bio-related substance.
    Type: Grant
    Filed: April 20, 2010
    Date of Patent: October 3, 2017
    Assignee: UNIVERSAL BIO RESEARCH CO., LTD.
    Inventor: Hideji Tajima