Detecting Nucleic Acid By Specific Antibody, Protein Or Ligand-receptor Binding Assay Patents (Class 435/6.19)
  • Patent number: 10940208
    Abstract: Nanoassembled complexes of nucleic acids, avidin and biotinylated compounds functionalized to make them suitable for delivering and releasing drugs are described. These nanoassembled complexes consist of central poly-avidin nucleus, i.e. nucleus formed from the self-assembling of tetrameric avidin units on oligonucleotide sequences of nucleic acids, on which biotinylated compounds are further auto-assembled through high-affinity interactions between the avidin of the central nucleus and the biotin of the biotinylated compounds. The biotinylated compounds are functionalized so as to bind drugs with a reversible bond, allowing the delivery thereof and a controlled release.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: March 9, 2021
    Assignee: UVERA SRL
    Inventor: Margherita Morpurgo
  • Patent number: 10913966
    Abstract: In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: February 9, 2021
    Assignee: Roswell Biotechnologies, Inc.
    Inventors: Barry L. Merriman, Venkatesh Alagarswamy Govindaraj, Paul Mola, Tim Geiser
  • Patent number: 10876112
    Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: December 29, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti
  • Patent number: 10871790
    Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: December 22, 2020
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Vladimir Shapiro, Stuart R. Ehrlich, Donald Francis Perrault, Jr., Blair D. Morad
  • Patent number: 10809180
    Abstract: Methods and devices for magnetic profiling of target particles in a flow. There are a plurality of flow rate-reducing structures in a flow chamber. Each flow rate-reducing structure is provided with a localized magnetic attractive force, the magnetic attractive force defining a capture zone in the vicinity of the flow rate-reducing structure. The size of capture zones may be variable for different locations within the device. The magnetic attractive force, in the capture zone, is sufficiently high to overcome the drag force on a given subset of the target particles to promote capture of any particles belonging to the subset of the target particles in the capture zone. Different target particles having different magnetic susceptibility are captured in different capture zones.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: October 20, 2020
    Inventors: Shana Kelley, Edward Sargent, Mahla Poudineh, Reza Mohamadi, Peter Aldridge
  • Patent number: 10780435
    Abstract: Disclosed is a minifluidic device including a matrix, an elongated guiding duct embedded at least in part in the matrix, with at least one port to the outside of the matrix, a movable fiber at least partly contained in the guiding duct, and able to undergo within the guiding duct, and at least along some part of the fiber, at least one action selected among a sliding, or a deformation, or a rotation and at least one of the movable fiber or the guiding duct is elastic or is non linear along at least part of its length, or at least part of the matrix is elastic.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: September 22, 2020
    Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT CURIE, UNIVERSITE PIERRE ET MARIE CURIE
    Inventors: Bastien Venzac, Ayako Yamada, Jean-Louis Viovy, Stephanie Descroix, Laurent Malaquin
  • Patent number: 10782240
    Abstract: The present disclosure provides methods and mechanisms for measuring small masses attached to a substrate within a microcantilever. Specifically, the disclosure describes the measurement of small particles accumulated at a substrate that cannot be flowed through a microchannel within a microcantilever. A resonance measurement is acquired at a first time. A pair resonance measurements is then acquired at a second point in time—one with the test mass at a first position off or along the microcantilever, the second with the test mass at a second position along the microcantilever. Comparing the resonance frequencies determined for the two test mass positions allows for disambiguation of changes in the mass of the particles from changes in the resonant behavior of the microcantilever itself.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: September 22, 2020
    Assignee: Applied Invention, LLC
    Inventor: W. Daniel Hillis
  • Patent number: 10775395
    Abstract: A method for performing a biological experiment includes accessing a protocol that specifies operations and prescribes a plurality of conditions for carrying out the biological experiment in a defined space. The method includes performing the sequence of operations according to the protocol; and periodically before or as the sequence of operations are performed, obtaining observations of particular conditions of the plurality, and comparing the particular conditions as observed to the particular conditions as prescribed.
    Type: Grant
    Filed: October 18, 2018
    Date of Patent: September 15, 2020
    Assignee: Arctoris Limited
    Inventors: Martin-Immanuel Bittner, Thomas Adam Fleming, Alice Poppy Roworth
  • Patent number: 10752937
    Abstract: Provided herein are porous polymer monolith materials and processes that enable integration of blood fractionation, specific nucleic acid amplification and/or detection of nucleic acids from whole blood.
    Type: Grant
    Filed: July 28, 2017
    Date of Patent: August 25, 2020
    Assignee: MONOLYTHIX, INC.
    Inventors: Ivan Rueda, Ian McFadden, Mark D. Dobbs, Victor Shum, Keith A. Oberg, Anthony Spence, Walt Caldwell
  • Patent number: 10741382
    Abstract: A method of forming a nanostructure comprises forming a directed self-assembly of nucleic acid structures on a patterned substrate. The patterned substrate comprises multiple regions. Each of the regions on the patterned substrate is specifically tailored for adsorption of specific nucleic acid structure in the directed self-assembly.
    Type: Grant
    Filed: October 31, 2017
    Date of Patent: August 11, 2020
    Assignee: Micron Technology, Inc.
    Inventors: Scott E. Sills, Gurtej S. Sandhu
  • Patent number: 10730892
    Abstract: Probes for formaldehyde (FA) including a homoallylamine trigger group attached to a detectable moiety are provided. Aspects of the probes include luminogenic or fluorogenic probes, such as a probe including a quencher in energy-receiving proximity to a fluorophore. Also provided are methods of using the probes for sensitive and bio orthogonal detection of FA in a sample. Aspects of the methods include selectively reacting the probe with the formaldehyde in the sample to a release (e.g., via a 2-aza-Cope rearrangement) a reporter group comprising a detectable moiety. Aspects of the methods detecting formaldehyde in a cell, tissue, organ or fluid in a subject. Also provided are compositions and kits including the subject probes that find use in practicing various embodiments of the subject methods.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: August 4, 2020
    Assignee: The Regents of the University of California
    Inventors: Christopher J. Chang, Thomas Francis Brewer, Jefferson Chan
  • Patent number: 10724143
    Abstract: There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: July 28, 2020
    Assignee: CustomArray, Inc.
    Inventors: Karl Maurer, John J. Cooper
  • Patent number: 10717084
    Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
    Type: Grant
    Filed: November 9, 2016
    Date of Patent: July 21, 2020
    Assignee: Georgia Tech Research Corporation
    Inventors: Todd Sulchek, Alexander Alexeev, Gonghao Wang
  • Patent number: 10689697
    Abstract: Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: June 23, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, Daniel John Turner, Andrew John Heron, Christopher James Wright
  • Patent number: 10683389
    Abstract: Provided herein are methods that comprise providing polymer chains comprising a plurality of reactive groups onto the surface of beads and covalent attachment of functionalized biomolecules, such as primers.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: June 16, 2020
    Assignee: GENAPSYS, INC.
    Inventors: Aldrich N. K. Lau, Paul M. Kenney, Henrik Persson
  • Patent number: 10676736
    Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.
    Type: Grant
    Filed: August 3, 2017
    Date of Patent: June 9, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti
  • Patent number: 10670505
    Abstract: A quantitative method of number surface area of a graphene material includes the following steps. The graphene material is mixed with a solution to form a colloidal solution containing the graphene material. The colloidal solution is atomized to form a plurality of aerosols containing the graphene material. The size of the aerosols is screened. The screened aerosols are counted to obtain a number concentration of the screened aerosols. A surface of the screened aerosols is charged and a current amount on the surface-charged aerosols is measured. The number surface area of the graphene material is calculated based on the current amount and the number concentration.
    Type: Grant
    Filed: April 11, 2018
    Date of Patent: June 2, 2020
    Assignee: National Tsing Hua University
    Inventors: De-Hao Tsai, Wei-Chang Chang
  • Patent number: 10617750
    Abstract: A Partner of Sld Five 1 (PSF1)-derived peptide that can induce a Cytotoxic T-Cell (CTL) response useful for a specific immunotherapy for cancer patients and a pharmaceutical composition for treating or preventing cancer, which contains the peptide according to the present invention are described.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: April 14, 2020
    Assignee: Vasculead Inc.
    Inventors: Hidekazu Tanaka, Motofumi Iguchi, Mari Yokoyama
  • Patent number: 10578603
    Abstract: A blood analyzer comprises a sample preparing part, a light source, a light receiving part, and a processing part configured to discriminate and count reactive B lymphocytes based on at least one of fluorescent light signals and forward scattered light signals.
    Type: Grant
    Filed: June 16, 2015
    Date of Patent: March 3, 2020
    Assignee: Sysmex Corporation
    Inventors: Konobu Kimura, Kinya Uchihashi, Jo Linssen, Jarob Saker
  • Patent number: 10569225
    Abstract: A technique for separating components of a microfluid, comprises a self-intersecting micro or nano-fluidic channel defining a cyclic path for circulating the fluid over a receiving surface of a fluid component separating member; and equipment for applying coordinated pressure to the channel at a plurality of pressure control areas along the cyclic path to circulate the fluid over the receiving surface, applying a pressure to encourage a desired transmission through the separating member, and a circulating pressure to remove surface obstructions on the separating member. The equipment preferably defines a peristaltic pump. Turbulent microfluidic flow appears to be produced.
    Type: Grant
    Filed: March 27, 2014
    Date of Patent: February 25, 2020
    Assignees: National Research Council of Canada, The Royal Institution for the Advancement of Learning/McGill University
    Inventors: Kebin Li, Teodor Veres, Tohid Fatanat Didar, Maryam Tabrizian
  • Patent number: 10564077
    Abstract: A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein to capture a desired fraction of the biological sample. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone multiple other positions adjacent various purification, protein analysis, separation and extraction zones. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the other zones to perform a multi-step assay on the isolated fraction within the device.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: February 18, 2020
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, Joshua M. Lang, Benjamin P. Casavant, Scott M. Berry, Lindsay N. Strotman
  • Patent number: 10550392
    Abstract: The present invention provides synthetic DNA aptamers that bind a target explosive to allow detection of that explosive. In various embodiments, the synthetic DNA aptamers may include one or more aptamers selected from the group consisting of SEQ ID 1-6. The various synthetic DNA aptamers are sensitive to different explosives. The synthetic DNA aptamers provide an inexpensive, in situ means for testing for explosive, which may be used for both soil and water samples. This testing may include an assay method using the synthetic DNA aptamers or a biosensor linked to the synthetic DNA aptamers.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: February 4, 2020
    Assignee: UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE ARMY
    Inventor: Jed O. Eberly
  • Patent number: 10464065
    Abstract: A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: November 5, 2019
    Assignee: ANDE Corporation
    Inventors: Richard F. Selden, Eugene Tan
  • Patent number: 10343164
    Abstract: Devices and methods for separating cells include a membrane that allows cells to pass from a first chamber to a second chamber.
    Type: Grant
    Filed: May 1, 2015
    Date of Patent: July 9, 2019
    Assignee: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY
    Inventors: Enzo Di Fabrizio, Gerardo Perozziello, Francesca Pardeo, Patrizio Candaloro
  • Patent number: 10266888
    Abstract: Methods of volumetric imaging of a three-dimensional matrix of nucleic acids within a cell is provided. An automated apparatus for sequencing and volumetric imaging of a three-dimensional matrix of nucleic acids is provided.
    Type: Grant
    Filed: May 2, 2018
    Date of Patent: April 23, 2019
    Assignee: President and Fellows of Harvard College
    Inventors: Evan R. Daugharthy, Richard C. Terry, Je-Hyuk Lee, George M. Church, Benjamin W. Pruitt
  • Patent number: 10227633
    Abstract: The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: March 12, 2019
    Assignee: BIGTEC PRIVATE LIMITED
    Inventors: Manoj Mulakkapurath Narayanan, Chandrasekhar Bhaskaran Nair
  • Patent number: 10220364
    Abstract: Disclosed herein is a method of: treating an organic polymer with an electron beam-generated plasma; exposing the treated polymer to air or an oxygen- and hydrogen-containing gas, generating hydroxyl groups on the surface of the polymer; reacting the surface with an organosilane compound having a chloro, fluoro, or alkoxy group and a functional or reactive group that is less reactive with the surface than the chloro, fluoro, or alkoxy group; and covalently immobilizing a biomolecule to the functional or reactive group or a reaction product thereof.
    Type: Grant
    Filed: April 13, 2018
    Date of Patent: March 5, 2019
    Assignee: The United States of America, as represented by the Secretary of the Navy
    Inventors: Stella H. North, Evgeniya H. Lock, Scott G. Walton, Chris Rowe Taitt
  • Patent number: 10217620
    Abstract: Hepatocellular carcinoma (HCC) is detected in a patient with liver disease. Mass spectrometry data from a blood-based sample from the patient is compared to a reference set of mass-spectrometry data from a multitude of other patients with liver disease, including patients with and without HCC, in a general purpose computer configured as a classifier. The classifier generates a class label, such as HCC or No HCC, for the test sample. A laboratory system for early detection of HCC in patients with liver disease is also disclosed. Alternative testing strategies using AFP measurement and a reference set for classification in the form of class-labeled mass spectral data from blood-based samples of lung cancer patients are also described, including multi-stage testing.
    Type: Grant
    Filed: June 27, 2018
    Date of Patent: February 26, 2019
    Assignee: Biodesix, Inc.
    Inventors: Joanna Röder, Carlos Oliveira, Julia Grigorieva, Heinrich Röder, Devalingam Mahalingam
  • Patent number: 10208342
    Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: February 19, 2019
    Assignee: The Regents of the University of California
    Inventors: William B. Dunbar, Noah A. Wilson, Mark A. Akeson, David W. Deamer, Kate Lieberman
  • Patent number: 10209250
    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 19, 2019
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 10203297
    Abstract: Described herein is a device comprising a plurality of first reaction electrodes arranged in an array, the plurality of first reaction electrodes configured to be exposed to a solution and having a capacitance; first circuitry configured to controllably connect the plurality of first reaction electrodes to a bias source and controllably disconnect the plurality of first reaction electrodes from the bias source; and second circuitry configured to measure a rate of charging or discharging of the capacitance. Also described herein is a method of using this device to sequence DNA.
    Type: Grant
    Filed: October 9, 2015
    Date of Patent: February 12, 2019
    Assignee: Intel Corporation
    Inventors: Jonathan S. Daniels, Oguz H. Elibol, Grace M. Credo, Xing Su
  • Patent number: 10202645
    Abstract: Devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore are provided. The devices and methods also determine (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: February 12, 2019
    Assignee: The Regents of the University of California
    Inventors: Mark A. Akeson, Daniel Branton, David W. Deamer, Seiko L. Endo
  • Patent number: 10152666
    Abstract: An authentication article includes: a substrate including: a first surface; a second surface disposed laterally to the first surface and at a depth below the first surface; and a plurality of indentations including the depth at the second surface of the substrate; and an array disposed on the substrate and including a plurality of analytes, the analytes being disposed in the indentations at a depth below a first surface of the substrate and provided to emit an authentication signature in response to being subjected to a probe stimulus. A process for authenticating the authentication article includes: providing the authentication article; subjecting the analytes to a probe stimulus; acquiring a response from the plurality of analytes in response to being subjected to the probe stimulus; and determining whether the response is the authentication signature to authenticate the, wherein the authentication article is not authenticated if the response is not the authentication signature for the array.
    Type: Grant
    Filed: August 24, 2015
    Date of Patent: December 11, 2018
    Assignee: THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE
    Inventors: Yaw S. Obeng, Joseph J. Kopanski, Jung-Joon Ahn
  • Patent number: 10117613
    Abstract: Tissue-integrating biosensors, systems comprising these sensors and methods of using these sensors and systems for the detection of one or more analytes are provided.
    Type: Grant
    Filed: March 31, 2016
    Date of Patent: November 6, 2018
    Assignee: Profusa, Inc.
    Inventors: Natalie A. Wisniewski, Kristen Helton, William A. McMillan
  • Patent number: 10094801
    Abstract: The present disclosure provides a bio-field effect transistor (BioFET) and a method of fabricating a BioFET device. The method includes forming a BioFET using one or more process steps compatible with or typical to a complementary metal-oxide-semiconductor (CMOS) process. The BioFET device may include a substrate; a gate structure disposed on a first surface of the substrate and an interface layer formed on the second surface of the substrate. The interface layer may allow for a receptor to be placed on the interface layer to detect the presence of a biomolecule or bio-entity. An amplification factor of the BioFET device may be provided by a difference in capacitances associated with the gate structure on the first surface and with the interface layer formed on the second surface.
    Type: Grant
    Filed: February 2, 2017
    Date of Patent: October 9, 2018
    Assignee: Taiwan Semiconductor Manufacturing Company, LTD.
    Inventors: Yi-Shao Liu, Rashid Bashir, Fei-Lung Lai, Chun-wen Cheng
  • Patent number: 10088443
    Abstract: Carriers for biomaterials, for polyelectrolyte materials, for electrically polarizable atoms, ions, molecules are provided wherein the material of the carriers is compatible with materials that are used in microelectronics. The arrangement of the biomaterials or biomolecules and optionally of biomolecules, biomaterials, biological functional units or cells adsorbed thereon can be affected with the carrier in a specific manner. Complex molecular machines can be built and tested by the carriers.
    Type: Grant
    Filed: August 31, 2012
    Date of Patent: October 2, 2018
    Assignees: HELMHOLTZ-ZENTRUM DRESDEN-ROSSENDORF E.V., LEIBNIZ-INSTITUT FÜR POLYMERFORSCHUNG DRESDEN E.V.
    Inventors: Heidemarie Schmidt, Christine Baumgart, Ilona Skorupa, Manfred Helm, Oliver G. Schmidt, Martin Müller
  • Patent number: 10081017
    Abstract: A method of quantifying nucleic acids in a sample includes generating a plurality of droplets in oil within a microfluidic device, wherein at least some of the droplets comprise a nucleic acid, amplification reagents, and a fluorescent probe or dyes contained therein. The droplets are delivered to a collection chamber to form an array of droplets. The droplets are subject to thermal cycling within the collection chamber a plurality of times to perform nucleic acid amplification within the droplets. The array of droplets is imaged during the plurality of thermal cycles as well as at a thermal cycle endpoint. An initial concentration of nucleic acid in the sample is calculated based on at least one of: a ratio of aqueous phase droplets exhibiting fluorescence within the array at the thermal cycle endpoint or a cycle threshold (Ct) of one or more aqueous phase droplets within the array.
    Type: Grant
    Filed: October 8, 2015
    Date of Patent: September 25, 2018
    Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
    Inventors: Abraham P. Lee, Andrew C. Hatch
  • Patent number: 10071374
    Abstract: A microfluidic device, for use in separation systems, includes a substrate having a fluidic channel. One or more heaters made of a thick film material are integrated with the substrate and in thermal communication with the fluidic channel of the substrate. The one or more heaters produce a thermal gradient within the fluidic channel in response to a current flowing through the one or more heaters. A plurality of electrically conductive taps can be in electrically conductive contact with the one or more heaters. The plurality of electrically conductive taps provides an electrically conductive path to the one or more heaters by which an electrical supply can produce the current flowing through the one or more heaters. Alternatively, the thick film material can be ferromagnetic, and the electrical supply can use induction to cause the current to flow through the one or more heaters.
    Type: Grant
    Filed: August 4, 2014
    Date of Patent: September 11, 2018
    Assignee: WATERS TECHNOLOGIES CORPORATION
    Inventors: Joseph D. Michienzi, James P. Murphy, Michael O. Fogwill, Keith Fadgen, Geoff Gerhardt, Gary W. Bertone
  • Patent number: 10059757
    Abstract: The invention provides antibodies and functional equivalents thereof which are capable of specifically binding RSV, and means and methods for producing them.
    Type: Grant
    Filed: February 18, 2016
    Date of Patent: August 28, 2018
    Assignee: MedImmune Limited
    Inventors: Hergen Spits, Tim Beaumont, Mark Jeroen Kwakkenbos, Etsuko Yasuda
  • Patent number: 10011134
    Abstract: A chemically etched two dimensional matrix symbol is provided on a substrate. The substrate may be metal or plastic. In another embodiment, a process is described for forming a plurality of two dimensional matrix symbols at the same time in batches. The two dimensional data matrix symbol comprises a substrate, a plurality of grooves and a plurality of raised areas and a plurality of ink spots disposed in the grooves according to a predefined code. The matrix is readable by a scanning device.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: July 3, 2018
    Assignee: Texas Nameplate Company, Inc.
    Inventors: Roy Dale Crownover, Ronnie Dwaine Phelps, Preston Cray Smith
  • Patent number: 9986941
    Abstract: Improved monitoring of one or more blood coagulation factors is provided. Factor-specific molecular probes having a dye-quencher arrangement are employed. In preferred embodiments, a compact integrated optical source and detector device is used for fluorescence measurements. Continuous and real-time monitoring of the activity of one or multiple blood factors is provided. Such real-time information can be used to automatically control delivery of drugs such as coagulants and/or blood thinners.
    Type: Grant
    Filed: June 26, 2015
    Date of Patent: June 5, 2018
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Meredith M. Lee, Jelena Levi, James L. Zehnder, James S. Harris, Jr.
  • Patent number: 9983155
    Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: May 29, 2018
    Assignees: Canon U.S. Life Sciences, Inc., CALIPER LIFE SCIENCES, INC.
    Inventors: Ivor T. Knight, Deborah John Boles, Aaron Rulison, Wesley B. Dong, Andrew Fabans, Allen Boronkay, Edward Donlon, Robert Moti, Michael Slater, Steven A. Sundberg, Michael R. Knapp
  • Patent number: 9983202
    Abstract: Disclosed is a method of synthesizing a polymeric fluorescent tracer for signal amplification, including (a) subjecting a streptavidin-fluorophore conjugate (SA-FL) and a spacer-attached biotin-conjugated antibody (b-Ab) to reaction at least two times; (b) treating a dual biotin oligomer spacer (db-oN); and (c) performing a homogenization process.
    Type: Grant
    Filed: January 23, 2017
    Date of Patent: May 29, 2018
    Assignee: Korea University Research and Business Foundation
    Inventors: Se-Hwan Paek, Sung-Min Seo
  • Patent number: 9970013
    Abstract: The present invention provides synthetic RNA aptamers that bind RDX. In various embodiments, the synthetic RNA aptamers may include one or more aptamers selected from the group consisting of SEQ ID 1-12. The synthetic RNA aptamers that bind RDX provide an inexpensive, in situ method for testing for RDX, which may be used for both soil and water samples.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: May 15, 2018
    Assignee: The United States of America as Represented by The Secretary of The Army
    Inventors: Jed O. Eberly, Fiona Crocker, Karl J. Indest
  • Patent number: 9964968
    Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.
    Type: Grant
    Filed: March 13, 2014
    Date of Patent: May 8, 2018
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Vladimir Shapiro, Stuart R. Ehrlich, Donald Francis Perrault, Jr., Blair D. Morad
  • Patent number: 9951313
    Abstract: The technology described herein is directed to methods and devices that can be used to induce functional organ structures to form within an implantation device by implanting it in vivo within the body of a living animal, and allowing cells and tissues to impregnate the implantation device and establish normal microenvironmental architecture and tissue-tissue interfaces. Then the contained cells and tissues can be surgically removed intact and either transplanted into another animal or maintained ex vivo by perfusing it through one or more of the fluid channels with medium and/or gases necessary for cell survival.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: April 24, 2018
    Assignees: President and Fellows of Harvard College, Children's Medical Center Corporation
    Inventors: Donald E. Ingber, Yusuke Torisawa, Geraldine Hamilton, Akiko Mammoto, Tadanori Mammoto, Catherine Spina
  • Patent number: 9951375
    Abstract: Methods, devices and systems for handling sample liquids, encapsulating liquids and magnetic particles are disclosed.
    Type: Grant
    Filed: June 18, 2014
    Date of Patent: April 24, 2018
    Assignee: Becton, Dickinson and Company
    Inventors: Kieran Curran, Robert Roeven
  • Patent number: 9919039
    Abstract: A Partner of Sld Five 1 (PSF1)-derived peptide that can induce a Cytotoxic T-Cell (CTL) response useful for a specific immunotherapy for cancer patients and a pharmaceutical composition for treating or preventing cancer, which contains the peptide according to the present invention are described.
    Type: Grant
    Filed: January 23, 2017
    Date of Patent: March 20, 2018
    Assignee: VASCULEAD INC.
    Inventors: Hidekazu Tanaka, Motofumi Iguchi, Mari Yokoyama
  • Patent number: 9910956
    Abstract: Methods and systems for single molecule sequencing using concatemers of copies of sense and antisense strands. Concatemers are provided, for example, by carrying out rolling circle amplification on a circular molecule having sense and antisense regions to produce repeated copies of the sense and antisense regions connected by linking regions. The circular molecules can be produced by ligating hairpin adapters to each end of a double-stranded nucleic acid having a sense and antisense strand. The ligations can be carried out, for example using blunt end ligation. In some cases, a single molecule consensus sequence for a single template molecule is obtained. A single read from each template molecule can be obtained by comparing the sequence information of the sense and antisense regions.
    Type: Grant
    Filed: August 19, 2016
    Date of Patent: March 6, 2018
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Kevin Travers, Geoff Otto, Stephen Turner, Cheryl Heiner, Congcong Ma
  • Patent number: RE47706
    Abstract: A remote microarray analysis system, method and apparatus for use in the remote analysis of a chemical compound microarray supported on a substrate is disclosed. Pixel image data is received from a remote location including image data that depicts (a) a calibration scale associated with the substrate and (b) the microarray. A transformation action of said pixel data corresponding to the calibration scale is determined and the received image data corresponding to at least the microarray is adjusted by applying the transformation action. The adjusted image of the microarray is compared with a database of stored microarray pixel data to extract information from said image.
    Type: Grant
    Filed: October 24, 2016
    Date of Patent: November 5, 2019
    Assignee: dacadoo ag
    Inventor: Peter Ohnemus