Detecting Nucleic Acid By Specific Antibody, Protein Or Ligand-receptor Binding Assay Patents (Class 435/6.19)
  • Patent number: 11959856
    Abstract: Methods and algorithms for a multiplexed single detection channel amplification process and quantification of generated amplicons is presented. Various mathematical approaches for quantifying and verifying the amplicons in a reaction are presented. Usage of such methods and approaches allow upgrading of existing single and multiple channel instruments for further multiplexing capabilities.
    Type: Grant
    Filed: September 11, 2017
    Date of Patent: April 16, 2024
    Assignee: California Institute of Technology
    Inventors: Imran R. Malik, Xiomara Linnette Madero, Erika F. Garcia, Sheel Mukesh Shah, Axel Scherer
  • Patent number: 11912970
    Abstract: Microfluidic devices, systems, and methods.
    Type: Grant
    Filed: May 15, 2018
    Date of Patent: February 27, 2024
    Inventors: Ce Zhang, Savas Tay
  • Patent number: 11834655
    Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.
    Type: Grant
    Filed: December 29, 2021
    Date of Patent: December 5, 2023
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti
  • Patent number: 11814488
    Abstract: A production method for a dried Vitrigel film includes, in the following order: step 1 of concentrically arranging and disposing member A having one or more recesses and member B having one or more through holes, such that the recesses overlap the through holes; step 2 of pouring a sol from the through holes of member B; step 3 of gelling the sol; step 4 of drying and vitrifying the hydrogel obtained in step 3 in a state in which it is formed in the member A and the member B; step 5 of hydrating the dried hydrogel obtained in step 4; step 6 of drying and re-vitrifying the Vitrigel obtained in step 5; and step 7 of cutting off a portion, which slightly covers the top surface of the member A, of the dried Vitrigel obtained in step 6.
    Type: Grant
    Filed: April 12, 2018
    Date of Patent: November 14, 2023
    Inventor: Toshiaki Takezawa
  • Patent number: 11788114
    Abstract: Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulse width of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
    Type: Grant
    Filed: July 12, 2019
    Date of Patent: October 17, 2023
    Inventors: Samad Talebpour, Aye Aye Khine, Robert Maaskant, Tino Alavie
  • Patent number: 11752499
    Abstract: A substrate assembly includes a first substrate, a second substrate and a bonding member. The first substrate includes a first surface-modified region having a functionality different from that of a remainder region of the first substrate. The second substrate includes a second surface-modified region connected to the first surface-modified region through a physical interaction and having a functionality different from that of a remainder region of the second substrate. The first and second substrates cooperatively define a space therebetween. The bonding member is disposed within said space to bond said first and second substrates together. A method for bonding substrates is also disclosed.
    Type: Grant
    Filed: October 21, 2021
    Date of Patent: September 12, 2023
    Assignee: Taiwan Green Point Enterprises Co., Ltd.
    Inventors: Sung-Yi Yang, Pao-Chuan Wang
  • Patent number: 11746372
    Abstract: Disclosed herein are method for separating, amplifying, or detecting a nucleic acid from a sample may comprise contacting a sample lysate with a plurality of buoyant, inorganic, nucleic-acid-capture microspheres. The nucleic-acid-capture microspheres may comprise unicellular hollow microspheres having a diameter between 5 and 300 ?m and/or a true particle density between 0.05 and 0.60 grams/cm3. The microspheres may comprise hollow soda-lime-borosilicate microspheres. In some embodiments, the microspheres comprises hollow soda-lime-borosilicate microspheres surrounded by an amorphous silica shell. Also disclosed are kits for performing the methods.
    Type: Grant
    Filed: December 3, 2018
    Date of Patent: September 5, 2023
    Assignees: GoDx, Inc., The United States of America, as Represented by the Secretary, Dep. of Health and Human Services
    Inventors: Chang Hee Kim, Lichen Xiang, Wendy A. Henderson, Xiao Jiang
  • Patent number: 11732298
    Abstract: Provided are methods for biological sample processing and analysis. A method can comprise providing a substrate configured to rotate. The substrate can comprise an array having immobilized thereto a biological analyte. A solution comprising a plurality of probes may be directed, via centrifugal force, across the substrate during rotation of the substrate, to couple at least one of the plurality of probes with the biological analyte. A detector can be configured to detect a signal from the at least one probe coupled to the biological analyte, thereby analyzing the biological analyte.
    Type: Grant
    Filed: May 20, 2019
    Date of Patent: August 22, 2023
    Assignee: Ultima Genomics, Inc.
    Inventors: Gilad Almogy, Kristopher Barbee, Nathan Beckett
  • Patent number: 11717828
    Abstract: Spatially distributed optical excitation and integrated waveguides are used for ultrasensitive particle detection based on individual electrokinetic velocities of particles. In some embodiments, chip-integrated systems are used to identify individual particles (e.g., individual molecules) based on their velocity as they move through an optically interrogated channel. Molecular species may be identified and quantified in a fully integrated setting, allowing for particle analysis including molecular analysis that can operate at low copy numbers down to the level of single-cell lysates. In some embodiments, the single-particle velocimetry-based identification and/or separation techniques are applied to various diagnostic assays, including nucleic acids, metabolites, macromolecules, organelles, cell, synthetic markers, small molecules, organic polymers, hormones, peptides, antibodies, lipids, carbohydrates, inorganic and organic microparticles and nanoparticles, whole viruses, and any combination thereof.
    Type: Grant
    Filed: December 19, 2017
    Date of Patent: August 8, 2023
    Inventors: Holger Schmidt, Aaron Roe Hawkins
  • Patent number: 11674971
    Abstract: The present invention relates to novel modules for transferring magnetic beads, an automated system comprising the same and a method for extracting nucleic acids using the same. The specifically designed magnet module and cover module of the present invention can be employed in the automated liquid handling apparatus by means of pre-existing moving modules (e.g., pipettor module) of the apparatus. The present invention enables a bead transfer-type method for extracting nucleic acids to be performed in an automated manner on the automated liquid handling apparatus. The present invention provides advantages of higher level of automation, more reduced cost and no need for another separate liquid handling apparatus compared to the conventional bead transfer-type method usually performed in the small apparatus designed to be used only for this bead transfer-type method. Also, the present method has the merits of more shortened reaction time compared to the conventional liquid transfer-type method.
    Type: Grant
    Filed: July 20, 2018
    Date of Patent: June 13, 2023
    Assignee: SEEGENE, INC.
    Inventors: Seung Jae Lee, Seong Sik Hong, Young Wook Kim
  • Patent number: 11566279
    Abstract: The present invention generally relates to a combination of molecular barcoding and emulsion-based microfluidics to isolate, lyse, barcode, and prepare nucleic acids from individual cells in a high-throughput manner.
    Type: Grant
    Filed: January 9, 2019
    Date of Patent: January 31, 2023
    Assignees: The Broad Institute, Inc., Massachusetts Institute of Technology, President and Fellows of Harvard College
    Inventors: Aviv Regev, Evan Zane Macosko, Steven Andrew McCarroll, Alexander K. Shalek, Anindita Basu, Christopher B. Ford, Hongkun Park, David A. Weitz
  • Patent number: 11536715
    Abstract: The present disclosure provides, inter alia, methods and compositions (e.g., conjugates) for imaging, at high spatial resolution, targets of interest.
    Type: Grant
    Filed: September 3, 2019
    Date of Patent: December 27, 2022
    Assignee: President and Fellows of Harvard College
    Inventors: Ralf Jungmann, Peng Yin, Mingjie Dai, Maier S. Avendano Amado, Johannes B. Woehrstein
  • Patent number: 11520227
    Abstract: An ejection material filling device fills an ejection material into a first storage space of an ejection material ejecting device including an ejection head configured to eject the ejection material and a storage container having an internal space separated by flexible membrane into the first storage space storing the ejection material and a second storage space storing a hydraulic fluid. The ejection material filling device includes an ejection material server bottle storing the ejection material to be filled into the first storage space, pipes configured to form a circulation path including the ejection material server bottle and the first storage space, a pump configured to circulate the ejection material through the circulation path, and a particle filter provided in the circulation path.
    Type: Grant
    Filed: April 22, 2020
    Date of Patent: December 6, 2022
    Assignee: Canon Kabushiki Kaisha
    Inventors: Yuichi Iwasaki, Yoshimasa Araki, Noriyasu Hasegawa, Hideki Matsumoto
  • Patent number: 11494164
    Abstract: An arithmetic processing apparatus includes a memory; and a processor coupled to the memory and the processor configured to execute a prediction process and a search process in an evolutionary calculation process for searching an optimum value of inputs by calculating an objective function based on eigen solutions for inputs and repeatedly calculating the objective function, wherein the prediction process includes predicting a range of an eigen solution for a second input, which satisfies a predetermined eigen solution condition, based on a first eigen solution for a first input when searches an optimum value of inputs by calculating an objective function based on eigen solutions for inputs and repeatedly calculating the objective function, and the search process includes searching a second eigen solution for the second input, which satisfies the eigen solution condition, in the predicted range of the eigen solution.
    Type: Grant
    Filed: January 30, 2020
    Date of Patent: November 8, 2022
    Inventor: Yukito Tsunoda
  • Patent number: 11426729
    Abstract: The present disclosure relates to systems and methods for whole cell analysis. In particular, the present disclosure relates to single cell genomic analysis (e.g., gene expression analysis.
    Type: Grant
    Filed: January 19, 2018
    Date of Patent: August 30, 2022
    Inventors: Euisik Yoon, Yu-Heng Cheng, Yu-Chih Chen, Riley Brien, Max S. Wicha
  • Patent number: 11390917
    Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.
    Type: Grant
    Filed: April 2, 2020
    Date of Patent: July 19, 2022
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: John Brian Hutchison, Jeffrey Charles Olson, Darren Roy Link
  • Patent number: 11371938
    Abstract: This disclosure relates to a sensor that detects bacteria cells comprising (a) a primary negatively charged, nanoparticulate sensing material; (b) a secondary positively charged, fluorescent sensing material; (c) a housing; and (d) at least one illuminator. This disclosure further relates to a method for detecting bacteria cells.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: June 28, 2022
    Inventors: Laura Sutarlie, Sian Yang Ow, Shilin Chen, Xiaodi Su
  • Patent number: 11366108
    Abstract: This invention relates to a method of producing a probe-bound carrier, a probe-bound carrier and a method of detecting or separating a target substance. The method of producing a probe-bound carrier includes: step 1 of mixing a carrier having tosyl groups and a probe; and step 2 of reducing the amount of tosyl groups on a surface of a carrier, in which a proportion of area S2 that is occupied by one tosyl group on a surface of the carrier obtained in the step 2 with respect to area S1A that is occupied by one tosyl group on a surface of the carrier used in the step 1 (S2/S1A×100%) is not less than 140%.
    Type: Grant
    Filed: March 27, 2018
    Date of Patent: June 21, 2022
    Inventors: Yuuichi Ueya, Yasuhito Satou
  • Patent number: 11366083
    Abstract: A detection cartridge, a detection method, and a detection device are provided. The detection cartridge includes a detection tank, a sample tank, N containers, and at least one first temporary tank. The sample tank is in communication with the detection tank. The N containers are in communication with the detection tank, wherein N is a positive integer greater than or equal to 2. The at least one first temporary tank is disposed on at least one of N flow paths between the N containers and the detection tank, wherein a quantity of the first temporary tanks on an nth flow path in the N flow paths is greater than or equal to that on an (n-1)th flow path, and n is a positive integer that is not less than 2 and is not more than N.
    Type: Grant
    Filed: September 11, 2019
    Date of Patent: June 21, 2022
    Assignee: SKYLA Corporation
    Inventor: Chia-Chun Wei
  • Patent number: 11351552
    Abstract: A reaction processor includes: a reaction processing vessel placing portion for placing a reaction processing vessel provided with a channel into which a sample is introduced; a temperature control system, which controls the temperature of the channel in order to heat the sample inside the channel; and a liquid feeding system, which controls the pressure inside the channel of the reaction processing vessel in order to move the sample inside the channel. The liquid feeding system maintains the pressure inside the channel to be higher than the atmospheric pressure in the surrounding of the reaction processing vessel, more preferably 1 atm or higher, during a reaction process of the sample.
    Type: Grant
    Filed: July 2, 2018
    Date of Patent: June 7, 2022
    Assignees: Nippon Sheet Glass Company, Limited, National Institute of Advanced Industrial Science and Technology
    Inventors: Takashi Fukuzawa, Hidenori Nagai
  • Patent number: 11346856
    Abstract: It is intended to save time for adjusting a position of a detection unit. In a position adjustment process of a detector, a control device moves the detector obliquely with respect to a boundary line partitioning photodiodes on a plane on which the detector moves and moves the detector so that the position of the center of gravity of a spot of a laser beam and the center of a light-receiving surface coincide in response to the incident of at least a part of the laser beam on the light-receiving surface.
    Type: Grant
    Filed: December 24, 2020
    Date of Patent: May 31, 2022
    Assignee: Shimadzu Corporation
    Inventor: Kenji Yamasaki
  • Patent number: 11345947
    Abstract: A microfluidic device includes a plurality of reaction wells; and a plurality of solid supports, and each of the solid supports has a reagent attached thereto. The reagent is attached to the solid support via a labile reagent/support bond such that the reagent is configured to be cleaved from the support via a cleaving operation.
    Type: Grant
    Filed: February 28, 2017
    Date of Patent: May 31, 2022
    Assignee: The University of North Carolina at Chapel Hill
    Inventors: John Michael Ramsey, William Henley, Emily Oblath
  • Patent number: 11340242
    Abstract: Provided are a quality control method, a quality control system, a management apparatus, an analyzer, and a quality control abnormality determination method in which measurement results of both a quality control substance and a specimen are sufficiently utilized to improve the quality of quality control. The quality control method used in a management apparatus which is connected via a network to an analyzer installed in each of a plurality of facilities includes obtaining, from an analyzer in each facility via a network, first quality control information obtained by measuring an artificially generated quality control substance, and second quality control information obtained by measuring a plurality of specimens by the analyzer in each facility; and outputting information concerning quality control of an analyzer in at least one facility, based on the obtained first quality control information and second quality control information.
    Type: Grant
    Filed: July 29, 2019
    Date of Patent: May 24, 2022
    Inventors: Keiji Fujimoto, Kazuhiko Matsuoka, Yasushi Hasui
  • Patent number: 11318469
    Abstract: An example system includes a microfluidic cavity; a retaining feature within the microfluidic cavity, and a releasing feature. The retaining feature is to position capsules at a predetermined location in the microfluidic cavity. The capsules have a thermally degradable shell enclosing a material therein. The releasing feature is to selectively cause degradation of the shell to release the material into the microfluidic opening. The releasing feature is to generate heat to facilitate degradation of the shell. In some examples, the retaining feature is a physical barrier sized to prevent flow of the capsule and to allow flow of the released materials through the microfluidic cavity.
    Type: Grant
    Filed: April 25, 2018
    Date of Patent: May 3, 2022
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Viktor Shkolnikov, Chien-Hua Chen
  • Patent number: 11305527
    Abstract: Provided herein are systems and methods for storing digital information by assembling an identifier nucleic acid molecule from at least a first component nucleic acid molecule and a second component nucleic acid molecule. The system may include a first printhead configured to dispense a first droplet of a first solution comprising the first component nucleic acid molecule onto a coordinate on a substrate, and a second printhead configured to dispense a second droplet of a second solution comprising the second component nucleic acid molecule onto the coordinate on the substrate, such that the first and second component nucleic acid molecules are collocated on the substrate. The system may include a finisher that dispenses a reaction mix onto the coordinate on the substrate to physically link the first and second component nucleic acid molecules, provides a condition necessary to physically link the first and second component nucleic acid molecules, or both.
    Type: Grant
    Filed: March 10, 2021
    Date of Patent: April 19, 2022
    Inventors: Nathaniel Roquet, Hyunjun Park, Swapnil P. Bhatia, Mike Hazell, Richard Day, Richard Hammond, James Brown, Rodney Richardson, Thomas Redman, Devin Leake
  • Patent number: 11300484
    Abstract: The disclosed invention is a new concept for a network of specialized continuous ambient air sampling systems, which employ a novel non-destructive ionization and separation method, coupled to a near real-time genomic sequencer. The network of preferably pathogen samplers, would constitute a plurality of distributed nodes throughout the world, with bacterial & viral concentration, identification, and mutation data uploaded to the cloud for epidemic/pandemic predictive modeling. The proposed system offers the ability to migrate from outbreak surveillance, to outbreak forecast. In addition, the capability for continuous data of genomic sequencing offers an enhanced capability to help track antigenic drift and antigenic shift. While optimized for viral capture and analysis, any airborne pathogen or spore can be accepted using the technology. Applications include world health monitoring, pandemic prediction, and detection of real-time bioterror pathogen deployment.
    Type: Grant
    Filed: October 16, 2017
    Date of Patent: April 12, 2022
    Assignee: Connecticut Analytical Corporation
    Inventor: Joseph J. Bango
  • Patent number: 11231373
    Abstract: A method according to one or more aspects may be a method of measuring a detection material contained in a sample by using a cartridge including: chambers each capable of housing at least one of the detection material and a reagent; and a path through which the detection material is transferred between the chambers. The method may include: moving at least one of the chambers and the path to a measurement position and an image capturing range by rotating the cartridge about a rotational shaft; measuring the detection material in the measurement position; and capturing an image of a monitoring target comprising at least one of the chambers and the path in the image capturing range.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: January 25, 2022
    Inventors: Tomoyuki Nose, Yusuke Muda, Kanako Nagaoka, Noriyuki Narisada, Varun Nambigari
  • Patent number: 11215595
    Abstract: Systems and methods for a mobile electronic system that gathers and analyzes odors, airborne chemicals and/or compounds. The system includes a sample delivery component that can gather airborne substances and/or gaseous substances. A detection component can detect the presences of chemicals, substances, and/or visual gases in a sample. Analyzed samples can be compared with known substance and/or odor analysis. In addition, the source of the sample can be determined. Accordingly, odor, gas, and/or airborne substance identification can be accomplished.
    Type: Grant
    Filed: May 15, 2020
    Date of Patent: January 4, 2022
    Inventors: Alexander Himanshu Amin, Himanshu Subhash Amin
  • Patent number: 11169139
    Abstract: To introduce a biomolecule into a nanopore without the need to check the position of the nanopore in a thin film. In addition, displacement stability is ensured and stable acquisition of blocking signals is realized. An immobilization member 107 having a larger size than a thin film 113 with a nanopore 112 is used, and biomolecules are immobilized on the biomolecule immobilization member 107 at a density that allows at least one biomolecule 108 to enter an electric field region around the nanopore when the biomolecule immobilization member 107 has moved close to a nanopore device 101.
    Type: Grant
    Filed: April 26, 2019
    Date of Patent: November 9, 2021
    Inventors: Rena Akahori, Itaru Yanagi, Kenichi Takeda
  • Patent number: 11162963
    Abstract: An automated sample specimen storage system including a tube holding microplate including a plate frame, a predetermined array of tube holding receptacles formed in the plate frame, the receptacles having a SBS standard pitch corresponding to the predetermined array, and being configured for holding therein sample store and transport tubes, each disposed so as to contain sample specimen in a sample storage of the storage system and to effect, with the sample tube, delivery from the sample storage to a workstation, the predetermined array of receptacles defining a volume capacity of the tube holding microplate, and each of the receptacles being shaped to conformally engage walls of the sample tubes and hold a respective one of the sample store and transport tubes, wherein the receptacles are arranged so that the tube holding microplate volume capacity defined by the predetermined array is an under optimum volume capacity.
    Type: Grant
    Filed: November 19, 2019
    Date of Patent: November 2, 2021
    Assignee: Brooks Automation, Inc.
    Inventors: Etienne Croquette, Robin Grimwood, David Andrew Harding, Chris Walsh
  • Patent number: 11079334
    Abstract: A food inspection apparatus includes: a conveyance unit; a light irradiation unit; an unit capturing an image of an inspection object A; a wavelength emphasis emphasizing a foreign substance specific wavelength characteristic of a foreign substance F from light having a wavelength of 300 nm to 1100 nm by using a first and/or second optical filter or a wavelength-specific light source; and an identification processing device identifying the foreign substance and including: a lightening unit normalizing the captured image with 256 or less of gradations into lightened data; and an identification unit having been provided with deep-learning of an identification processing of the foreign substance specific wavelength from the lightened data and identifying the foreign substance or a good item S in line from the lightened data obtained through capturing the image during conveyance of the inspection object.
    Type: Grant
    Filed: August 22, 2017
    Date of Patent: August 3, 2021
    Inventors: Kotaro Furihata, Takeshi Ogino, Kenji Suzuki, Hiromu Suzuki, Taketoshi Yamamoto, Mitsuhisa Ota, Yoshimitsu Imazu, Alejandro Javier Gonzalez Tineo, Yuta Yoshida, Yohei Sugawara
  • Patent number: 11073512
    Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.
    Type: Grant
    Filed: November 28, 2018
    Date of Patent: July 27, 2021
    Assignee: Enzo Life Sciences, Inc.
    Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
  • Patent number: 11060095
    Abstract: The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.
    Type: Grant
    Filed: December 10, 2020
    Date of Patent: July 13, 2021
    Inventors: Weijuan Yang, Haixiang Yu, Yingzhu Liu, Yi Xiao
  • Patent number: 11008610
    Abstract: A multiplex slide plate device and an operation method thereof are provided. The multiplex slide plate device includes a slide plate and a sacrificial layer. The slide plate has reaction vessels arranged in an array, an injection hole and an exhaust hole, wherein each of the reaction vessels has an opening portion and a bottom portion. The sacrificial layer has a microfluidic channel, wherein the microfluidic channel has an injection channel, a main channel and a distal channel connected to each other. The sacrificial layer is assembled to the slide plate, wherein the main channel faces the opening portion. A sample solution is injected into the injection channel, such that the sample solution flows from the injection channel through the main channel to the distal channel, wherein the sample solution loads into each of the reaction vessels while flowing through the main channel.
    Type: Grant
    Filed: November 15, 2018
    Date of Patent: May 18, 2021
    Assignee: Quark Biosciences Taiwan, Inc.
    Inventors: Chung-Fan Chiou, Cheng-Wey Wei, Yu Chang, Ming-Chuan Hsu
  • Patent number: 11009464
    Abstract: Methods, apparatuses, and computer program products are provided where fluid, such as a blood sample, is entered into a microfluidic channel in a microchip where the microfluidic channel possesses a micro/nanopillar array for sorting molecules by size. When the fluid passes through the micro/nanopillar array it is separated into particles of interest or particles not of interest or both. When particles of interest are lit by a light source via a first waveguide in the microchip connecting the light source to the microfluidic channel, then lighted particles of interest can be detected by an optical detector via a second waveguide in the microchip connecting the optical detector to the microfluidic channel. The information from the optical detector can be analyzed further by connecting the microchip to a mobile computing device with its own processing abilities or abilities via the internet or cloud.
    Type: Grant
    Filed: December 11, 2015
    Date of Patent: May 18, 2021
    Assignee: International Business Machines Corporation
    Inventors: Huan Hu, Oki Gunawan, Ning Li, Devendra K. Sadana, Joshua T. Smith, William T. Spratt, Yann Astier
  • Patent number: 10940208
    Abstract: Nanoassembled complexes of nucleic acids, avidin and biotinylated compounds functionalized to make them suitable for delivering and releasing drugs are described. These nanoassembled complexes consist of central poly-avidin nucleus, i.e. nucleus formed from the self-assembling of tetrameric avidin units on oligonucleotide sequences of nucleic acids, on which biotinylated compounds are further auto-assembled through high-affinity interactions between the avidin of the central nucleus and the biotin of the biotinylated compounds. The biotinylated compounds are functionalized so as to bind drugs with a reversible bond, allowing the delivery thereof and a controlled release.
    Type: Grant
    Filed: July 8, 2016
    Date of Patent: March 9, 2021
    Assignee: UVERA SRL
    Inventor: Margherita Morpurgo
  • Patent number: 10913966
    Abstract: In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.
    Type: Grant
    Filed: May 19, 2020
    Date of Patent: February 9, 2021
    Assignee: Roswell Biotechnologies, Inc.
    Inventors: Barry L. Merriman, Venkatesh Alagarswamy Govindaraj, Paul Mola, Tim Geiser
  • Patent number: 10876112
    Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.
    Type: Grant
    Filed: November 18, 2019
    Date of Patent: December 29, 2020
    Assignee: Bio-Rad Laboratories, Inc.
    Inventors: Ronald Lebofsky, Jeremy Agresti
  • Patent number: 10871790
    Abstract: The present disclosure provides improved particle processing (e.g., cytometry and/or cell purification) systems and methods that can operate in an autonomous fashion. More particularly, the present disclosure provides for assemblies, systems and methods for analyzing, sorting, and/or processing (e.g., purifying, measuring, isolating, detecting and/or enriching) particles (e.g., cells, microscopic particles, etc.) where human intervention is not required and/or is minimized. The systems, assemblies and methods of the present disclosure advantageously improve run performance of particle processing systems (e.g., cell purification systems, cytometers) by significantly reducing and/or substantially eliminating the burden of operation for human intervention by automating numerous functions, features and/or steps of the disclosed systems and methods.
    Type: Grant
    Filed: May 7, 2018
    Date of Patent: December 22, 2020
    Assignee: CYTONOME/ST, LLC
    Inventors: Johnathan Charles Sharpe, Nemanya Sedoglavich, Vladimir Shapiro, Stuart R. Ehrlich, Donald Francis Perrault, Jr., Blair D. Morad
  • Patent number: 10809180
    Abstract: Methods and devices for magnetic profiling of target particles in a flow. There are a plurality of flow rate-reducing structures in a flow chamber. Each flow rate-reducing structure is provided with a localized magnetic attractive force, the magnetic attractive force defining a capture zone in the vicinity of the flow rate-reducing structure. The size of capture zones may be variable for different locations within the device. The magnetic attractive force, in the capture zone, is sufficiently high to overcome the drag force on a given subset of the target particles to promote capture of any particles belonging to the subset of the target particles in the capture zone. Different target particles having different magnetic susceptibility are captured in different capture zones.
    Type: Grant
    Filed: September 27, 2016
    Date of Patent: October 20, 2020
    Inventors: Shana Kelley, Edward Sargent, Mahla Poudineh, Reza Mohamadi, Peter Aldridge
  • Patent number: 10780435
    Abstract: Disclosed is a minifluidic device including a matrix, an elongated guiding duct embedded at least in part in the matrix, with at least one port to the outside of the matrix, a movable fiber at least partly contained in the guiding duct, and able to undergo within the guiding duct, and at least along some part of the fiber, at least one action selected among a sliding, or a deformation, or a rotation and at least one of the movable fiber or the guiding duct is elastic or is non linear along at least part of its length, or at least part of the matrix is elastic.
    Type: Grant
    Filed: July 22, 2016
    Date of Patent: September 22, 2020
    Inventors: Bastien Venzac, Ayako Yamada, Jean-Louis Viovy, Stephanie Descroix, Laurent Malaquin
  • Patent number: 10782240
    Abstract: The present disclosure provides methods and mechanisms for measuring small masses attached to a substrate within a microcantilever. Specifically, the disclosure describes the measurement of small particles accumulated at a substrate that cannot be flowed through a microchannel within a microcantilever. A resonance measurement is acquired at a first time. A pair resonance measurements is then acquired at a second point in time—one with the test mass at a first position off or along the microcantilever, the second with the test mass at a second position along the microcantilever. Comparing the resonance frequencies determined for the two test mass positions allows for disambiguation of changes in the mass of the particles from changes in the resonant behavior of the microcantilever itself.
    Type: Grant
    Filed: May 4, 2017
    Date of Patent: September 22, 2020
    Assignee: Applied Invention, LLC
    Inventor: W. Daniel Hillis
  • Patent number: 10775395
    Abstract: A method for performing a biological experiment includes accessing a protocol that specifies operations and prescribes a plurality of conditions for carrying out the biological experiment in a defined space. The method includes performing the sequence of operations according to the protocol; and periodically before or as the sequence of operations are performed, obtaining observations of particular conditions of the plurality, and comparing the particular conditions as observed to the particular conditions as prescribed.
    Type: Grant
    Filed: October 18, 2018
    Date of Patent: September 15, 2020
    Assignee: Arctoris Limited
    Inventors: Martin-Immanuel Bittner, Thomas Adam Fleming, Alice Poppy Roworth
  • Patent number: 10752937
    Abstract: Provided herein are porous polymer monolith materials and processes that enable integration of blood fractionation, specific nucleic acid amplification and/or detection of nucleic acids from whole blood.
    Type: Grant
    Filed: July 28, 2017
    Date of Patent: August 25, 2020
    Assignee: MONOLYTHIX, INC.
    Inventors: Ivan Rueda, Ian McFadden, Mark D. Dobbs, Victor Shum, Keith A. Oberg, Anthony Spence, Walt Caldwell
  • Patent number: 10741382
    Abstract: A method of forming a nanostructure comprises forming a directed self-assembly of nucleic acid structures on a patterned substrate. The patterned substrate comprises multiple regions. Each of the regions on the patterned substrate is specifically tailored for adsorption of specific nucleic acid structure in the directed self-assembly.
    Type: Grant
    Filed: October 31, 2017
    Date of Patent: August 11, 2020
    Assignee: Micron Technology, Inc.
    Inventors: Scott E. Sills, Gurtej S. Sandhu
  • Patent number: 10730892
    Abstract: Probes for formaldehyde (FA) including a homoallylamine trigger group attached to a detectable moiety are provided. Aspects of the probes include luminogenic or fluorogenic probes, such as a probe including a quencher in energy-receiving proximity to a fluorophore. Also provided are methods of using the probes for sensitive and bio orthogonal detection of FA in a sample. Aspects of the methods include selectively reacting the probe with the formaldehyde in the sample to a release (e.g., via a 2-aza-Cope rearrangement) a reporter group comprising a detectable moiety. Aspects of the methods detecting formaldehyde in a cell, tissue, organ or fluid in a subject. Also provided are compositions and kits including the subject probes that find use in practicing various embodiments of the subject methods.
    Type: Grant
    Filed: August 18, 2016
    Date of Patent: August 4, 2020
    Assignee: The Regents of the University of California
    Inventors: Christopher J. Chang, Thomas Francis Brewer, Jefferson Chan
  • Patent number: 10724143
    Abstract: There is disclosed an electrochemical deblocking solution for use on an electrode microarray. There is further disclosed a method for electrochemical synthesis on an electrode array using the electrochemical deblocking solution. The solution and method are for removing acid-labile protecting groups for synthesis of oligonucleotides, peptides, small molecules, or polymers on a microarray of electrodes while substantially improving isolation of deblocking to active electrodes. The method comprises applying a voltage or a current to at least one electrode of an array of electrodes. The array of electrodes is covered by the electrochemical deblocking solution.
    Type: Grant
    Filed: June 22, 2018
    Date of Patent: July 28, 2020
    Assignee: CustomArray, Inc.
    Inventors: Karl Maurer, John J. Cooper
  • Patent number: 10717084
    Abstract: Microfluidic devices for cell sorting or cell fractionation are disclosed. A microfluidic device can comprise one or more inlets, a first wall and a second wall, and two or more outlets. The first and second walls can be substantially planar to each other and the first wall having can have a plurality of ridges protruding from the first wall and defining a compression gap between the ridge and a surface of the second wall. The microfluidic device can also be a cell sorting device for sorting a plurality of cells based on one or more biophysical cellular properties including size, elasticity, viscosity, and/or viscoelasticity wherein the cells are subjected to one or more compressions due to the compression gap. Also disclosed are methods for cell sorting based on a variety of biophysical cellular properties.
    Type: Grant
    Filed: November 9, 2016
    Date of Patent: July 21, 2020
    Assignee: Georgia Tech Research Corporation
    Inventors: Todd Sulchek, Alexander Alexeev, Gonghao Wang
  • Patent number: 10689697
    Abstract: Analysis Of A Polymer A biochemical analysis system analyses polymers by taking measurements of a polymer from a sensor element comprising a nanopore during translocation of the polymer through the nanopore. When a polymer has partially translocated, the series of measurements is analysed using reference data derived from a reference sequence to provide a measure of similarity. Responsive to the measure of similarity, the sensor element may be selectively operated to eject the polymer and thereby make the nanopore available to receive a further polymer. Where the biochemical analysis system comprises an array of sensor elements and is takes measurements from sensor elements selected in a multiplexed manner, responsive to the measure of similarity, the biochemical analysis system ceases taking measurements from the currently selected sensor element and to starts taking measurements from a newly selected sensor element.
    Type: Grant
    Filed: October 16, 2015
    Date of Patent: June 23, 2020
    Assignee: Oxford Nanopore Technologies Ltd.
    Inventors: Stuart William Reid, Gavin Harper, Clive Gavin Brown, Daniel John Turner, Andrew John Heron, Christopher James Wright
  • Patent number: 10683389
    Abstract: Provided herein are methods that comprise providing polymer chains comprising a plurality of reactive groups onto the surface of beads and covalent attachment of functionalized biomolecules, such as primers.
    Type: Grant
    Filed: September 18, 2017
    Date of Patent: June 16, 2020
    Assignee: GENAPSYS, INC.
    Inventors: Aldrich N. K. Lau, Paul M. Kenney, Henrik Persson