Involving Avidin-biotin Binding Patents (Class 435/7.5)
  • Patent number: 11320418
    Abstract: A hand-held point of care monitoring system that includes a plurality of assay modules configured to receive different assay devices that perform assays on one or more samples. At least two of the assay modules or at least two of the assay devices have different identifiers that identify the assays. The system also includes an apparatus having a portable frame configured to interchangeably receive the plurality of assay modules in a same port; a means for decoding the different identifiers when received by the frame; and a means for reading assay results.
    Type: Grant
    Filed: August 24, 2020
    Date of Patent: May 3, 2022
    Assignee: iAssay, Inc.
    Inventor: Lonnie W. Adelman
  • Patent number: 11313855
    Abstract: The present invention relates to methods and compositions to remove or reduce biotin interference from certain assays.
    Type: Grant
    Filed: September 19, 2019
    Date of Patent: April 26, 2022
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventor: Tie Wei
  • Patent number: 11209429
    Abstract: The present invention provides a method for increasing the sensitivity of LFIAs by using palladium nanoparticles, selecting appropriate dye chemistries, and improving the timing of the development chemistry. In the presence of a palladium nanoparticle, three reagents interact with a catalytic label to form a colored dye. The three reagents include a hydrogen peroxide source, a color developer (a substituted para-phenylenediamine), and a color coupler (e.g. a napthol or a phenol). The timing of the development chemistry is improved by any combination of using a reducing agent, delaying hydrogen peroxide application by diffusion, using dissolving materials as a time delay, using serpentine flow, and separating the color coupler and the color developer on the strip.
    Type: Grant
    Filed: May 2, 2019
    Date of Patent: December 28, 2021
    Assignee: The Government of the United States of America, as represented by the Secretary of the Navy
    Inventor: David A. Kidwell
  • Patent number: 11162138
    Abstract: Sets of compounds bearing detectably different groups of labels are provided. Typically, different compounds bear different numbers of a single type of label and are thus distinguishable by the amplitude of signal produced by the label. The compounds are assembled from label components and protein cores to facilitate modular production of the compounds. In compounds containing two or more proteins, the proteins are typically covalently linked. Useful sets of compounds include sets of labeled nucleotide analogs, particularly dye-label nucleotide analogs that include tetravalent biotin-binding protein cores.
    Type: Grant
    Filed: October 24, 2018
    Date of Patent: November 2, 2021
    Assignee: Pacific Biosciences of California, Inc.
    Inventors: Erik Miller, Satwik Kamtekar
  • Patent number: 11114184
    Abstract: Methods and systems for obtaining inhibitors of human DNA methyltransferase 1 (DNMT1) are disclosed where the methods involve designing compounds that resemble the DNMT1 transition state.
    Type: Grant
    Filed: February 13, 2018
    Date of Patent: September 7, 2021
    Inventors: Vern L. Schramm, Quan Du, Zhen Wang
  • Patent number: 11103519
    Abstract: The invention relates to BODIPY derivatives of Formula (I): that bear one or more functional groups which in the photochemical reaction upon irradiation with visible light undergo deamination and deliver quinone methides. Furthermore, the invention relates to the antiproliferative activity of BODIPY derivatives and their use for pharmaceutical applications and for fluorescent labeling, particularly for labeling proteins.
    Type: Grant
    Filed: May 15, 2017
    Date of Patent: August 31, 2021
    Inventors: Nikola Basaric, Marijeta Kralj, Ana-Matea Mikecin, Matej Cindric
  • Patent number: 11085932
    Abstract: The present invention relates to biological markers of metabolic bone diseases or disorders such as osteoporosis and related methods using thereof. The invention further relates to the use of those biomarkers and related material and assays in the diagnosis of subjects at risk of developing such disorders or monitoring the effects of a treatment thereof.
    Type: Grant
    Filed: October 7, 2016
    Date of Patent: August 10, 2021
    Inventors: Serge Ferrari, Nicolas Bonnet, Daniel S. Spellman
  • Patent number: 10802022
    Abstract: Two Borrelia burgdorferi recombinant proteins were expressed in E. coli. These two proteins were generated from (a) the full length dbpA gene combined with the invariable region 6 of the VlsE gene (dbpA/C6), and (b) the full length OspC gene combined with the coding sequence for amino acids 1-121 of the E. coli maltose binding protein gene (OspC/MBP). Methods of using these recombinant proteins for detecting anti-Borrelia burgdorferi antibodies in patient sera and diagnosis of Lyme Disease are described.
    Type: Grant
    Filed: August 29, 2019
    Date of Patent: October 13, 2020
    Assignee: Ross Southern Laboratories
    Inventors: Gary L. Bradshaw, R. Kelley Thueson, Todd Joseph Uriona
  • Patent number: 10613101
    Abstract: A new method of in vitro monitoring and assessing the need of a medication which interferes with the regulation of the parathyroid hormone level in a kidney patient subject to oxidative stress, notably hemodialysis patients. FIG. 1 shows the distribution of n-oxPTH concentrations in 340 hemodialysis patients (224 men and 116 women) with a median age of 66 years (IQR, 56 to 75 years), a median time since initiation of dialysis (dialysis vintage) of 266 days (IQR, 31 to 1209 days), and a median dialysis dose (kt/V) of 1.2 (IQR, 1.1 to 1.3). The cause of chronic kidney disease was nephrosclerosis in 113 cases (33%), diabetic nephropathy in 107 cases (31%), chronic glomerular nephritis in 29 cases (9%), polycystic kidney disease in 9 cases (3%) and other/unknown in 82 cases (24%). The median n-oxPTH concentration was 5.9 ng/L (IQR, 2.4 to 14.0 ng/L). n-oxPTH concentrations were not different in men and women (5.9 ng/L; IQR, 2.4 to 14.2 ng/L; n=224; vs. 5.5 ng/L; IQR, 2.4 to 14.0 ng/L; n=116; p=0.915).
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: April 7, 2020
    Assignee: Immundiagnostik AG
    Inventors: Franz Paul Armbruster, Berthold Hocher, Heinz Jürgen Roth
  • Patent number: 10464894
    Abstract: The present disclosure provides conjugate structures and hydrazinyl-pyrrolo compound structures used to produce these conjugates. The disclosure also encompasses methods of production of such conjugates, as well as methods of using the same.
    Type: Grant
    Filed: September 26, 2016
    Date of Patent: November 5, 2019
    Assignee: Redwood Biosciences, Inc.
    Inventors: David Rabuka, Aaron Edward Albers, Romas Alvydas Kudirka, Albert W. Garofalo
  • Patent number: 10094822
    Abstract: An object of the invention is to obtain a biotinylated nucleic acid efficiently, by enhancing dissociation efficiency of biotin in the biotinylated nucleic acid and tamavidin 2 in a tamavidin 2-immobilized insoluble carrier. The inventive method for separating a biotinylated nucleic acid includes (1) contacting a sample containing a biotinylated nucleic acid wherein the biotin is bound to the nucleic acid with a insoluble carrier on which tamavidin is immobilized (a tamavidin-immobilized insoluble carrier) to form a complex of the biotinylated nucleic acid and the tamavidin-immobilized insoluble carrier, and (2) separating the biotinylated nucleic acid from the complex in a solution having pH of 7.8 to 9.5 and in the presence of free biotin. The invention also provides a method for separating the biotinylated nucleic acid to which the nucleic acid-binding protein is bound, a method for separating the nucleic acid-binding protein, and a kit for separating the nucleic acid.
    Type: Grant
    Filed: December 17, 2015
    Date of Patent: October 9, 2018
    Inventor: Ryo Ukekawa
  • Patent number: 10030067
    Abstract: This invention relates to compounds (e.g., those delineated herein), pharmaceutically acceptable salts, prodrugs, solvates, and hydrates thereof. This invention also provides compositions comprising a compound of this invention and the use of such compounds and compositions in methods of treating diseases and conditions that are beneficially treated by administering modulating compounds or compositions that modulate A?, PAMPS and DAMPS.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: July 24, 2018
    Assignee: University of Florida Research Foundation, Incorporated
    Inventors: Todd E. Golde, Paramita Chakrabarty
  • Patent number: 9797879
    Abstract: Disclosed herein is a method of generating a high resolution image of a cellular sample, the method including i) labeling a cellular sample with at least one mass tag, thereby producing a labeled sample in which a biological feature of interest is associated with the at least one mass tag, ii) scanning the sample with a continuous or near-continuous primary ion beam to generate sputtered secondary ions and sputtered neutral species, iii) photoionizing the sputtered neutrals to generate ionized neutral species, wherein the sputtered neutrals are photoionized at a site that is proximal to their source on the sample, iv) detecting the ionized neutral species by mass spectrometry, thereby obtaining spatially addressed measurements of the abundance of the at least one mass tag across an area of the sample, and v) producing an image of the sample using the measurements. A system for performing the method is also provided.
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: October 24, 2017
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventor: Robert M. Angelo
  • Patent number: 9783594
    Abstract: The invention provides broadly neutralizing antibodies directed to epitopes of Human Immunodeficiency Virus, or HIV. The invention further provides compositions containing HIV antibodies used for prophylaxis, and methods for diagnosis and treatment of HIV infection.
    Type: Grant
    Filed: May 17, 2012
    Date of Patent: October 10, 2017
    Assignees: The Rockefeller University, California Institute of Technology
    Inventors: Johannes Scheid, Michel Nussenzweig, Pamela J. Bjorkman, Ron Diskin
  • Patent number: 9766245
    Abstract: The present invention provides a method for labeling or detecting a protein with certain glycosyl groups. The methods are particularly useful for detecting cancer cells comprising the detected glycosyl groups. The present invention further provides labeling agents and detection agents, labeled proteins and mixtures, and kits and arrays thereof.
    Type: Grant
    Filed: January 3, 2014
    Date of Patent: September 19, 2017
    Assignee: California Institute of Technology
    Inventors: Linda C. Hsieh-Wilson, Wen Yi, Jean-Luc Chaubard
  • Patent number: 9625451
    Abstract: The present invention provides a method for detecting chronic sinusitis, which comprises measuring the concentration of a periostin protein in blood or nasal secretion collected from a test subject. Thereby, a method for detecting chronic sinusitis, which is capable of detecting chronic sinusitis more simply, more promptly and less invasively, is provided.
    Type: Grant
    Filed: October 30, 2012
    Date of Patent: April 18, 2017
    Inventors: Kenji Izuhara, Shoichiro Ohta, Kazuhiko Arima, Hiroshi Shiraishi, Shoichi Suzuki, Nobuo Ohta, Akihiro Ishida, Yusuke Suzuki
  • Patent number: 9574220
    Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.
    Type: Grant
    Filed: March 11, 2015
    Date of Patent: February 21, 2017
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Allen E Eckhardt, Carrie Graham, Ramakrishna Sista, Vamsee K. Pamula, Theodore Winger, Tong Wang, Yalin Xiong, Ning Wu, Sanjay Saha, Gajendrasinh Raolji, Raveendra Dayam
  • Patent number: 9555103
    Abstract: The present invention relates to a nutritional, preferably enteral, composition for use in the treatment of cow's milk allergy in infants allergic to cow's milk containing specific beta-lactoglobulin peptides, which are able to reduce, in particular abolish the acute symptoms of cow's milk protein allergy.
    Type: Grant
    Filed: December 6, 2012
    Date of Patent: January 31, 2017
    Assignee: N.V. NUTRICIA
    Inventors: Léon Mathieu Johannes Knippels, Johann Garssen, Laura Antoinette Petronella Maria Muelenbroek
  • Patent number: 9433718
    Abstract: This disclosure relates to medical fluid sensors and related systems and methods. In some aspects, a medical fluid cartridge includes a body including a first portion defining a first fluid passageway and a second portion defining a second fluid passageway in fluid communication with the first fluid passageway. The body defines a gap between the first portion of the body and the second portion of the body such that the first portion of the body can be disposed in a radio frequency device while the second portion of the body remains outside the radio frequency device.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: September 6, 2016
    Assignee: Fresenius Medical Care Holdings, Inc.
    Inventor: Ross Peter Jones
  • Patent number: 9310380
    Abstract: Provided are a detection method for a myriad of proteins involved in an autoimmune disease with high sensitivity and high efficiency, and an analysis method for data resulting from the detection method. In order to construct the detection method and analysis method, there is provided means for comprehensively analyzing the proteins involved in an autoimmune disease by bringing a mammal-derived protein expressed in a cell-free protein synthesis system into contact with a sample derived from a patient with an autoimmune disease to detect autoantibody production, and subjecting the detected data to statistical analysis processing, and further, gene ontology analysis and/or pathway analysis.
    Type: Grant
    Filed: November 16, 2011
    Date of Patent: April 12, 2016
    Assignees: National University Corporation Ehime University, Public University Corporation Yokohama City University
    Inventors: Tatsuya Sawasaki, Yaeta Endo, Tomoaki Ishigami, Ichiro Aoki
  • Patent number: 9174999
    Abstract: Saxitoxin analog compounds, compositions, pharmaceutical compositions, methods of synthesis of saxitoxin analogs, methods of imaging, methods of treatment, including methods of treating pain, are provided. Saxitoxin (STX), gonyautoxin (GTX), and zetekitoxin, and variant STX compounds bind to sodium channels and are effective to reduce or block flow of sodium ions through such channels. Such channel block affects nerve and muscle action, and may be effective to reduce or block pain sensations, relax muscles, reduce muscle spasm, and reduce wrinkles. STX analog binding to sodium channels may also be useful to locate, image, or mark sodium channels, and so be useful in studying sodium channels and sodium channel disorders, and in the diagnosis and treatment of patients suffering from sodium channel disorders. In embodiments, the variant STX compounds include conjugates having increased serum half-life as compared to STX when administered to a subject.
    Type: Grant
    Filed: May 7, 2010
    Date of Patent: November 3, 2015
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: Justin Du Bois, John Mulcahy, Brian Andresen, David C. Yeomans, Sandip Biswal
  • Patent number: 9168294
    Abstract: The invention provides RSV fusion (F) protein ectodomain polypeptide sequences and nucleotide sequences encoding them, as well as cells containing the invention's polypeptide and nucleotide sequences. The invention further provides VLPs that contain the invention's polypeptides, and methods for using the VLPs for protein expression and vaccine formulation. Also provided are methods for distinguishing between subjects immunized with the invention's compositions and subjects infected with RSV.
    Type: Grant
    Filed: November 8, 2013
    Date of Patent: October 27, 2015
    Inventor: Trudy Morrison
  • Patent number: 9040251
    Abstract: This invention provides a biomolecule modifying substrate comprising biomolecules selectively fixed to given regions thereon. The biomolecule modifying substrate comprises: a substrate at least comprising a first surface and a second surface; a first linker molecule comprising a hydrocarbon chain and a functional group capable of selectively binding to the first surface at one end of the hydrocarbon chain, which is bound to the first surface via such functional group; a second linker molecule comprising a reactive group capable of binding to the hydrocarbon chain of the first linker molecule, which is bound to the first linker molecule via a bond between the reactive group and the hydrocarbon chain; and a biomolecule bound thereto via the second linker molecule.
    Type: Grant
    Filed: July 29, 2010
    Date of Patent: May 26, 2015
    Inventors: Yasuhiko Tada, Hiroshi Yoshida, Toshiro Saito, Masatoshi Narahara, Hiroaki Nakagawa
  • Patent number: 9034590
    Abstract: The present invention provides a method for detecting a substance in a biological sample, a carrier for using in the method, and a kit.
    Type: Grant
    Filed: March 31, 2010
    Date of Patent: May 19, 2015
    Inventors: Yoshimitsu Takakura, Naomi Oka, Kazuhiro Kondo
  • Publication number: 20150118692
    Abstract: Acoustically responsive particles and methods are provided for their use. Methods are provided for making and using tunable, monodisperse acoustically responsive particles and negative contrast acoustic particles, wherein the particles can contain a functional group available for covalent modification.
    Type: Application
    Filed: March 15, 2013
    Publication date: April 30, 2015
    Inventors: Leah M. Johnson, Gabriel P. Lopez, Lu Gao, Charles Wyatt Shields, IV
  • Patent number: 9018360
    Abstract: The present invention provides a modified biotin-binding protein comprising an amino acid sequence represented by SEQ ID NO: 2 or its modified sequence and having a biotin-binding activity and replacement selected from the group consisting of: 1) replacement of the 36th serine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 2) replacement of the 80th tryptophan residue of SEQ ID NO: 2 with a hydrophilic amino acid residue; 3) replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 4) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 78th threonine residue of SEQ ID NO: 2 with an amino acid residue that does not form a hydrogen bond; 5) replacement of the 46th proline residue of SEQ ID NO: 2 with a threonine, serine, or tyrosine residue and replacement of the 116th aspartic acid residue of SEQ ID NO: 2 with an amino acid that
    Type: Grant
    Filed: June 24, 2009
    Date of Patent: April 28, 2015
    Assignee: Japan Tobacco Inc.
    Inventors: Yoshimitsu Takakura, Masako Tsunashima, Kozue Sofuku
  • Publication number: 20150111765
    Abstract: Biosensor components (chips) are described based on direct biocoating processes that result in the tenacious and stable, noncovalent (believed to be chemisorptive) binding of anchor substances such as avidin(s) other proteins having specific binding partners or oligo- or poly-nucleotides onto any piezo-electrically active crystal surface. The resulting platform technology can be developed for a variety of biosensors with specific applications in biological assays. The table mono layers of the anchor substances forms reactive layers, ready to bind a capture reagent such as a biot-inylated antibody for capture and detection of analytes in biologic fluid samples.
    Type: Application
    Filed: August 27, 2012
    Publication date: April 23, 2015
    Inventors: Lisa Laury-Kleintop, Herman Rutner
  • Patent number: 9005910
    Abstract: The purpose is to produce, with high reproducibility, a complex of labeled probes and a carrier, said complex being to be used for detecting and measuring a target substance to be measured with high sensitivity and high stability. The means for accomplishing the purpose is that a label is bound to a probe-water soluble carrier conjugate using specific binding of an avidin compound such as avidin, streptavidin, etc. to biotin, and the binding of the avidin compound to the probe is performed before the binding to the carrier. Namely, after conjugating the avidin compound to a substance which is capable of binding to the target substance, the conjugate is bound to a high-molecule water-soluble carrier to produce a complex of the avidinized probes and the water-soluble carrier. Then the complex of the avidinized probes and the water-soluble carrier is mixed with a biotinylated label.
    Type: Grant
    Filed: April 13, 2011
    Date of Patent: April 14, 2015
    Assignees: Eiken Kagaku Kabushiki Kaisha, Advanced Life Science Institute, Inc.
    Inventors: Yoshiyuki Ohiro, Susumu Takayasu
  • Patent number: 8999739
    Abstract: An apparatus comprises: a sensing element formed on a buried oxide layer of a substrate and providing communication between a source region and a drain region; a gate dielectric layer on the sensing element, the gate dielectric layer defining a sensing surface on the sensing element; a passive surface surrounding the sensing surface; and a compound bound to the sensing surface and not bound to the passive surface, the compound having a ligand specifically configured to preferentially bind a target molecule to be sensed. An electrolyte solution in contact with the sensing surface and the passive surface forms a top gate of the apparatus.
    Type: Grant
    Filed: August 13, 2013
    Date of Patent: April 7, 2015
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Christopher P. D'Emic, Ashish Jagtiani, Sufi Zafar
  • Patent number: 8994077
    Abstract: An apparatus comprises: a sensing element formed on a buried oxide layer of a substrate and providing communication between a source region and a drain region; a gate dielectric layer on the sensing element, the gate dielectric layer defining a sensing surface on the sensing element; a passive surface surrounding the sensing surface; and a compound bound to the sensing surface and not bound to the passive surface, the compound having a ligand specifically configured to preferentially bind a target molecule to be sensed. An electrolyte solution in contact with the sensing surface and the passive surface forms a top gate of the apparatus.
    Type: Grant
    Filed: December 21, 2012
    Date of Patent: March 31, 2015
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Christopher P. D'Emic, Ashish Jagtiani, Sufi Zafar
  • Patent number: 8993227
    Abstract: Disclosed are composition and methods for treating development-related disorders. Also disclosed are diagnosis methods, prognosis methods, and drug screening methods.
    Type: Grant
    Filed: April 9, 2013
    Date of Patent: March 31, 2015
    Inventors: Zhenglun Zhu, Hong Gao
  • Publication number: 20150080250
    Abstract: A solid support for detecting the presence of an analyte in a sample at or above a predetermined threshold comprises: a competitive analyte within said solid support, wherein said competitive analyte is coupled to a first member of a binding pair; a labelled conjugate within said solid support, downstream of said competitive analyte, wherein said analyte and said competitive analyte compete for binding to said conjugate; and a capture reagent immobilized within said solid support, downstream of said conjugate, wherein said capture reagent comprises a second member of the binding pair; wherein the affinity of the first and second members of the binding pair for one another and the distance between the conjugate and the capture reagent and/or the conjugate and the competitive analyte within the solid support are selected so as to increase the threshold of the solid support for the analyte.
    Type: Application
    Filed: April 19, 2013
    Publication date: March 19, 2015
    Applicant: ZBX Corporation
    Inventors: Qinwei Shi, Jing Xu
  • Publication number: 20150064718
    Abstract: An organic/inorganic composite is provided. The organic/inorganic composite materials are sufficiently hard and brittle to be ground to form particles suitable for biological or chemical separation applications. The organic/inorganic composite materials can be magnetic or magnetically susceptible and can have functional reactive groups to allow attachment of biomolecules. Methods of making and using the organic/inorganic composites are also provided.
    Type: Application
    Filed: August 28, 2014
    Publication date: March 5, 2015
    Inventors: Stephen Joseph Caracci, Martin Joseph Murtagh, Christopher Bankole Shogbon, Yue Zhou
  • Publication number: 20150064717
    Abstract: Provided is a method for staining a tissue enabling highly precise staining, by which the expression amount and/or the location of a biological substance in a tissue sample can be detected with a high quantitativity together with detailed information that can be obtained by bright field observation. The tissue staining method of the present invention is a method for staining a tissue, in which both staining that allows bright field observation and fluorescence staining are carried out for the same specific biological substance.
    Type: Application
    Filed: March 26, 2013
    Publication date: March 5, 2015
    Inventors: Hideki Gouda, Hideki Hoshino, Kensaku Takanashi, Yasushi Nakano, Kohsuke Gonda, Noriaki Ohuchi, Mika Watanabe
  • Patent number: 8945857
    Abstract: The invention provides methods for isolating cells, particularly antibody-secreting cells that have a high likelihood of secreting antibodies specific for a desired antigen for the purpose of making monoclonal antibodies.
    Type: Grant
    Filed: June 30, 2006
    Date of Patent: February 3, 2015
    Inventor: John Schrader
  • Patent number: 8945947
    Abstract: A method for determining the binding constant of interacting molecular species comprises the use of flat surfaces comprising perfluorinated polymers and measurements of reflected light intensity. The surfaces comprise at least one molecule with the receptor function absorbed or immobilized on the surface and at least one ligand that interacts with the receptor.
    Type: Grant
    Filed: January 31, 2011
    Date of Patent: February 3, 2015
    Assignee: Solvay Solexis S.p.A.
    Inventor: Mattia Bassi
  • Publication number: 20150031626
    Abstract: A method of diagnosing metastatic potential of a breast cancer in an individual with breast cancer is described. The method comprises a step of assaying a breast cancer tumour sample from the patient for expression of ADAM22, wherein expression of ADAM22 correlates with increased potential for metastasis compared with a patient who is ADAM22 negative. The invention also describes an agent for use in the treatment of metastatic breast cancer in a patient, in which the agent is selected from LGI1 protein (SEQ ID NO:1) and an LGI1 peptide mimic capable of mimicking the ADAM22 binding activity of LGI1 by binding to the LGI1 binding domain of ADAM22 (SEQ ID NO: 4) and which is capable of inhibiting migration of endocrine resistant breast cancer cells.
    Type: Application
    Filed: November 5, 2012
    Publication date: January 29, 2015
    Applicant: Royal College of Surgeons in Ireland
    Inventors: Leonie Young, Damian McCartan, Christopher Byrne
  • Publication number: 20150011433
    Abstract: Multi-biotinylated reactants are provided which can be used in divalent complexes for various applications such as colocalization, labeling, immobilization, and purification. Methods for constructing, purifying, and using the bis-biotinylated reactants are also provided. In certain embodiments, two bis-biotinylated reactants are bound to a single streptavidin tetramer to provide a complex having a 1:1 stoichiometry with respect to the bis-biotinylated reactants.
    Type: Application
    Filed: June 12, 2014
    Publication date: January 8, 2015
    Applicant: Pacific Biosciences of California, Inc.
    Inventors: Erik Miller, Satwik Kamtekar, Keith Bjornson, Jeremiah Hanes
  • Publication number: 20140378558
    Abstract: Methods for detecting colorectal cancer in a subject by detection of a galactose-containing 40 kDa molecule in a serum sample from the subject. Methods for quantifying the amount of a galactose-containing molecule in a serum sample are also provided.
    Type: Application
    Filed: October 10, 2012
    Publication date: December 25, 2014
    Inventors: Robert Bresalier, Nachman Mazurek, James C. Byrd
  • Patent number: 8906637
    Abstract: The present invention provides method and compositions for detecting target molecules present on cells and tissues. In particular, the methods involve adding primary antibodies such as scFv-targeted lactamase that are directed against a target of interest (e.g., cancer markers) to a tissue sample, followed by adding a lactam-containing compound and finally a lactamase reporter system. In some preferred embodiments, the lactamase reporters are fluorescent reporters that bind to the test tissue. In some particularly preferred embodiments, the test tissue contains at least once cancer cell and/or at least one cancer-associated marker.
    Type: Grant
    Filed: September 9, 2005
    Date of Patent: December 9, 2014
    Assignee: Danisco US Inc.
    Inventor: Steven W. Sherwood
  • Patent number: 8900825
    Abstract: The invention provides novel immunogenic proteins LigA and LigB from Leptospira for use in the development of effective vaccines and antibodies, as well as improved diagnostic methods and kits.
    Type: Grant
    Filed: April 30, 2012
    Date of Patent: December 2, 2014
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Yung-Fu Chang, Raghavan U. M. Palaniappan
  • Publication number: 20140349893
    Abstract: The invention describes methods for labeling or detecting of immobilized poly(amino acids), including peptides, polypeptides and proteins, on membranes and other solid supports, using fluorescent dipyrrometheneboron difluoride dyes. Such immobilized poly(amino acids) are labeled or detected on blots or on arrays of poly(amino acids), or are attached to immobilized aptamers.
    Type: Application
    Filed: April 25, 2014
    Publication date: November 27, 2014
    Inventors: Richard HAUGLAND, Karen MARTIN, Wayne PATTON
  • Patent number: 8895259
    Abstract: The invention lies in the area of platelet function diagnostics and relates to an in vitro method for the determination of platelet function under flow conditions. The method is particularly suitable for the determination of the effect of clopidogrel after oral intake and of other P2Y(12) antagonists with antithrombotic activity as well as the determination of P2Y(1) receptor antagonists with antithrombotic activity.
    Type: Grant
    Filed: April 26, 2007
    Date of Patent: November 25, 2014
    Assignee: Siemens Healthcare Diagnostics Products GmbH
    Inventor: Andreas Rechner
  • Patent number: 8889370
    Abstract: A homogeneous method of determining inhibitors of proteolytically active coagulation factors (anticoagulants) in a sample, in particular direct thrombin and factor Xa inhibitors, and also a test kit to be used in such a method. Use is made of ligands which bind to the proteolytically active coagulation factor but are not cleaved by the latter and compete with the anticoagulant to be determined.
    Type: Grant
    Filed: December 21, 2011
    Date of Patent: November 18, 2014
    Assignee: Siemens Healthcare Diagnostics Products GmbH
    Inventors: Andreas Kappel, Andreas Rechner, Sina Stephan, Thomas Wissel
  • Publication number: 20140273010
    Abstract: The present disclosure relates to biological probes useful for detecting the presence of a target molecule. The biological probes are capable of forming complexes with the target molecule that are stable to reduction, oxidation and hydrolysis.
    Type: Application
    Filed: March 14, 2014
    Publication date: September 18, 2014
    Applicant: The Research Foundation for The State University of New York
    Inventors: Nicole Sampson, Siyeon Lee
  • Publication number: 20140273009
    Abstract: The disclosure relates to compositions for use in assays, the compositions comprising at least one latent fluorophore including at least one enzyme-reactive quenching group and a conjugative group; and a support connectable to the latent fluorophore by the conjugative group. The disclosure further relates to methods of measuring the presence and/or concentration of an analyte, as well as methods of measuring the relative activity of at least two enzymes.
    Type: Application
    Filed: March 14, 2013
    Publication date: September 18, 2014
    Applicant: Palo Alto Research Center Incorporated
    Inventors: Michael I. RECHT, Peter KIESEL, Joerg MARTINI, Francisco E. TORRES
  • Patent number: 8835125
    Abstract: Disclosed are compositions and methods for the labeling of two or more targets with different labels. Specifically, disclosed are compositions for biotin and the protection of biotin within multilabel assays which employ the biotin-biotin binding protein binding relationship for each distinct label in relation to targets such as nucleic acids, polypeptides, antibodies or cells. These multilabel assays are enabled through the use of biotin with desthiobiotin, orthogonal protecting schemes for biotin, or a combination of the approaches.
    Type: Grant
    Filed: December 16, 2011
    Date of Patent: September 16, 2014
    Assignee: Affymetrix, Inc.
    Inventors: Robert G. Kuimelis, Glenn H. McGall, Stephen P. A. Fodor
  • Patent number: 8835121
    Abstract: Provided herein is a modified method of agglutination to detect infections caused by microorganisms including the steps of staining the test serum, plasma or blood or purified antibodies with a protein stain; mixing serum, plasma or blood with stained antibodies with an equal quantity of colored antigen particles on a glass slide; adding diluted Antiglobulin conjugated with Biotin to the mixture; subjecting the mixture to the step of mixing, adding diluted Avidin (preferably tagged with a visible indicator) to the mixture and thoroughly mixing all the ingredients.
    Type: Grant
    Filed: September 16, 2010
    Date of Patent: September 16, 2014
    Assignees: Department of Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University
    Inventor: Hari Mohan Saxena
  • Patent number: 8835360
    Abstract: A method for selecting combinations of drugs for treatment of diseases that arise from deranged signaling pathways is disclosed. The method involves measuring the activity states for signaling proteins in a diseased cell and determining whether the activity states are different from the activity states observed for a reference cell such as a normal cell. Based on the observed differences, combinations of two or more drugs are selected to reduce these differences. Treatment of a subject with the combinations restores the activity states of the signaling proteins of the deranged disease-associated signaling pathways toward the activity states observed in the reference cell. Since the diseased cell and the reference cell can both be obtained from the same subject, combinations of drugs that specifically target patient-specific signaling derangements is possible.
    Type: Grant
    Filed: April 24, 2012
    Date of Patent: September 16, 2014
    Assignee: The United States of America as represented by the Secretary of the Department of HHS
    Inventors: Arpita I. Mehta, Lance A. Liotta, Emanuel F. Petricoin
  • Patent number: 8822168
    Abstract: The present invention includes assays and kits for detecting the assembly of an RNA binding protein-RNA complex and for detecting the activity of an RNA binding protein.
    Type: Grant
    Filed: March 10, 2006
    Date of Patent: September 2, 2014
    Assignee: The Trustees of the University of Pennsylvania
    Inventors: Gideon Dreyfuss, Lili Wan, Elizabeth Ottinger