Using A Micro-organism To Make A Protein Or Polypeptide Patents (Class 435/71.1)
  • Patent number: 11912637
    Abstract: Certain exemplary embodiments can provide a system, machine, device, manufacture, circuit, composition of matter, and/or user interface adapted for and/or resulting from, and/or a method and/or machine-readable medium comprising machine-implementable instructions for, activities that can include and/or relate to, converting biomass to synthetic hydrocarbons using a biomass thermal decomposer and/or a hydrocarbon synthesizer.
    Type: Grant
    Filed: July 11, 2023
    Date of Patent: February 27, 2024
    Inventor: Matthew D. Merrill
  • Patent number: 11866751
    Abstract: Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding an alpha-amylase and/or a heterologous polynucleotide encoding a trehalase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms.
    Type: Grant
    Filed: July 22, 2019
    Date of Patent: January 9, 2024
    Assignee: Novozymes A/S
    Inventors: Michael Glenn Catlett, Monica Tassone, Paul Vincent Harris, Robert Lyle Osborne, Ryoko Kataoka, Shiro Fukuyama, Tomoko Matsui
  • Patent number: 11821016
    Abstract: An objective of the present invention is to provide a culturing method, by which Bacillus bacterial spores can be efficiently produced. The present invention provides a method for producing Bacillus bacterial spores, comprising culturing Bacillus bacteria in a liquid medium containing a sugar or a sugar-source raw material at a concentration between 50.1 g/L and 100 g/L at the start of culture, and then in the course of the culture, feeding a fed-batch medium containing a sugar or a sugar-source raw material and a nitrogen-containing compound, and having the weight ratio (C/N ratio) of carbon atoms to nitrogen atoms ranging from 5.5 to 13.5 to the liquid medium.
    Type: Grant
    Filed: October 6, 2017
    Date of Patent: November 21, 2023
    Assignee: SDS BIOTECH K.K.
    Inventor: Yuki Tsukagoshi
  • Patent number: 11771111
    Abstract: Described herein are methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
    Type: Grant
    Filed: June 17, 2021
    Date of Patent: October 3, 2023
    Assignee: Impossible Foods Inc.
    Inventors: Carl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
  • Patent number: 11773156
    Abstract: Provided herein are monoclonal antibodies, or antigen-binding fragments thereof, that bind to the influenza B hemagglutinin (HA) protein, pharmaceutical compositions comprising the antibodies and methods of use. The antibodies are useful for inhibiting or neutralizing influenza B virus activity, thus providing a means of treating or preventing influenza infection in humans. Also provided is the use of one or more antibodies that bind to the influenza B HA for preventing viral attachment and/or entry into host cells. The antibodies may be used prophylactically or therapeutically and may be used alone or in combination with one or more other anti-viral agents or vaccines.
    Type: Grant
    Filed: October 28, 2020
    Date of Patent: October 3, 2023
    Assignee: REGENERON PHARMACEUTICALS, INC.
    Inventors: Alina Baum, Christos Kyratsous
  • Patent number: 11718644
    Abstract: The present disclosure provides methods for producing consumable recombinant proteins that are substantially free from herein-disclosed undesired byproducts.
    Type: Grant
    Filed: October 27, 2022
    Date of Patent: August 8, 2023
    Assignee: CLARA FOODS CO.
    Inventors: Aniket Kale, Ranjan Patnaik
  • Patent number: 11643665
    Abstract: Methods and compositions for modulating shoot apical meristem size are provided. Methods are provided for modulating the expression of fea3 sequence in a host plant or plant cell to modulate agronomic characteristics such as altered size and number of organs, including plant seeds.
    Type: Grant
    Filed: February 6, 2020
    Date of Patent: May 9, 2023
    Assignees: CORTEVA AGRISCIENCE LLC, COLD SPRING HARBOR LABORATORY
    Inventors: Stephen M Allen, David Peter Jackson, Byoung Il Je, Mai Komatsu, Young Koung Lee, Hajime Sakai
  • Patent number: 11639388
    Abstract: Provided in the present application is a humanized and multifunctional CD3 antibody; a heavy chain variable region of the CD3 antibody comprised herein contains the amino acid sequence of any one of SEQ ID NOs: 1-3 or a variant sequence thereof; and a light chain variable region thereof contains the amino acid sequence of any one of SEQ ID NOs: 26-28 or a variant sequence thereof. Further provided in the present application is a multifunctional antibody comprising (a) a light chain-heavy chain pair that has specificity for tumor cells or microorganisms; and (b) a fusion peptide that comprises a single-chain variable fragment and an Fc fragment having a CH2 domain and/or a CH3 domain, the fusion peptide having specificity for immune cells. The antibodies provided in the present application have improved biological activity, thermal stability and/or acid resistance.
    Type: Grant
    Filed: February 22, 2019
    Date of Patent: May 2, 2023
    Assignee: Wuhan YZY Biopharma Co., Ltd.
    Inventors: Jing Zhang, Lijuan Fang, Yongxiang Yan, Liang Zeng, Pengfei Zhou
  • Patent number: 11613559
    Abstract: The present invention provides a nucleic acid construct comprising the following structure: A-X-B in which A is nucleic acid sequence encoding a first polypeptide which comprises a first signal peptide; B is nucleic acid sequence encoding a second polypeptide which comprises a second signal peptide and X is a nucleic acid sequence which encodes a cleavage site, wherein the first signal peptide or the second signal peptide comprises one or more mutation(s) such that it has fewer hydrophobic amino acids.
    Type: Grant
    Filed: April 26, 2016
    Date of Patent: March 28, 2023
    Assignee: AUTOLUS LIMITED
    Inventors: Martin Pulé, Shaun Cordoba
  • Patent number: 11596704
    Abstract: The present disclosure relates to an article for detecting a disinfectant via visual feedback. The article have a first substrate with a first major surface and opposite ends. The article also comprises a process indicator disposed on at least a portion of the first major surface. The process indicator reacts with at least one liquid disinfectant selected from the group consisting of glutaraldehyde, ortho-phthalaldehyde, hydrogen peroxide, peroxyacetic acid, and combinations thereof. The process indicator can be formed from a synthetic amine-containing polymer derived from polyethylenimine (PEI). The article can have a flow channel that is formed by a portion of the process indicator and that extends between the opposite ends. The disclosure also relates to a kit containing the article as well as a method of using the article in a disinfection process. The article is used to diagnose issues in an automated endoscope reprocessors (AERs).
    Type: Grant
    Filed: December 7, 2017
    Date of Patent: March 7, 2023
    Assignee: 3M Innovative Properties Company
    Inventors: Timothy J. Nies, G. Marco Bommarito, Christopher J. Claypool
  • Patent number: 11473050
    Abstract: The invention relates to a method for the protein enrichment of a heterotrophically cultured microalga, the microalga being of the genus Chlorella, even more particularly Chlorella protothecoides, characterized in that it comprises: a first step directed toward limiting the ammonium supply so as to obtain a microalgal biomass with a protein content of less than 50% expressed as N.6.25, preferably less than 30%, more preferentially between 20 and 25%; a second step in which the ammonium supply in the fermentation medium is increased so as to obtain a protein content of greater than 50%, preferably greater than 60%, more preferentially greater than 65%.
    Type: Grant
    Filed: February 8, 2016
    Date of Patent: October 18, 2022
    Assignee: Corbion Biotech, Inc.
    Inventors: Marie Le Ruyet, Laurent Segueilha, Mélanie Cappe, Sylvain Delaroche
  • Patent number: 11440855
    Abstract: The invention relates to the use of phycobiliproteins or of an extract containing same as fertilizer, a method for stimulating tillering and/or root development and/or the yield of a plant, as well as fertilizer compositions comprising phycobiliproteins or an extract containing same and (i) an amendment and/or (ii) a fertilizer other than phycobiliproteins or an extract containing same.
    Type: Grant
    Filed: December 10, 2018
    Date of Patent: September 13, 2022
    Assignee: AGRO INNOVATION INTERNATIONAL
    Inventors: Jean-Claude Yvin, Florence Cruz, Mariana Carolina Pereira Vieira Devault, Larissa Villar
  • Patent number: 11427847
    Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.
    Type: Grant
    Filed: January 29, 2021
    Date of Patent: August 30, 2022
    Assignee: Novozymes, Inc.
    Inventors: Suchindra Maiyuran, Abigail Jang, Kimberly Brown, Sandra Merino, Jeffrey Shasky, Eric Abbate
  • Patent number: 11401348
    Abstract: Immunoglobulin compositions that simultaneously co-engage antigens, where one of the antigens is bound bivalently and the other antigen is bound monovalently. The novel immunoglobulins described preferably utilize heterodimeric Fc regions.
    Type: Grant
    Filed: August 13, 2019
    Date of Patent: August 2, 2022
    Assignee: Xencor, Inc.
    Inventors: Gregory Lazar, Gregory Moore
  • Patent number: 11098332
    Abstract: The present invention relates to a method for the enzymatic reduction of cystine to cysteine comprising contacting cystine with a reduction solution comprising: (i) an active glutathione reductase (EC1.8.1.7); (ii) a cofactor; and (iii) glutathione; and recovering a cysteine comprising composition, wherein the reduction solution has a pH of at least 6 during contacting with cystine.
    Type: Grant
    Filed: December 14, 2017
    Date of Patent: August 24, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Marco Alexander Van Den Berg, Elaheh Jamalzadeh, Samuel Adrianus Maria Ruinard
  • Patent number: 11078474
    Abstract: A method is for the extraction of a phytochemical, e.g., peptide or protein, or a chemical element from a macroalgae, by applying pulsed electric field (PEF)- or continuous electric field (CEF)-treatment to the macroalgae in a solvent, under pressure higher than the ambient pressure. A device and system carry out this method.
    Type: Grant
    Filed: January 30, 2018
    Date of Patent: August 3, 2021
    Assignee: Ramot at Tel-Aviv University Ltd.
    Inventors: Alexander Golberg, Arthur Robin
  • Patent number: 10993976
    Abstract: A medicament for use in treating uremia and proteinuria, the medicament being a glycoprotein, a mixture of polysaccharide and protein, a polypeptide or a protein.
    Type: Grant
    Filed: January 20, 2017
    Date of Patent: May 4, 2021
    Assignee: SHANDONG ZHONGHAI PHARMACEUTICAL CO. LTD
    Inventors: Qian Cheng, Baozhen Xu, Long Cheng
  • Patent number: 10952956
    Abstract: The present invention relates to an antioxidant composition for the skin. More specifically, it relates to an antioxidant composition for the skin having: Saccharomyces ferment; and ?-ketoglutaric acid or niacinamide alone or a mixture thereof. The antioxidant composition for the skin according to the present invention has not only an excellent antioxidant effect but also a high long lasting effect. Therefore, it provides an enhanced antioxidant effect when applied to the skin and it enables manufacture of various products such as cosmetics and medicines because it is not harmful to the human body.
    Type: Grant
    Filed: June 26, 2017
    Date of Patent: March 23, 2021
    Assignee: AMOREPACIFIC CORPORATION
    Inventors: Hyeon Chung Kim, Min Jeong Song, Eun Jung Lee, Nok Hyun Park, Ji Hyun Kim, Ji Yeong Kim
  • Patent number: 10927344
    Abstract: The present invention relates to cell culture in bioreactors, such as flexible cellbag bioreactors. More specifically the present invention relates to methods for simplifying the production of clinically relevant cell products for use in cell therapy.
    Type: Grant
    Filed: May 27, 2016
    Date of Patent: February 23, 2021
    Assignee: Cytiva Sweden AB
    Inventors: Karin Molleryd, Karin Wernersson, Sarah Marie Stone, Claudia Nune, Rolf Kiessling, Tanja Lovgren, Lars Adamsson, Ulrica Eistrand
  • Patent number: 10737953
    Abstract: A system for enhancing the separation of particles or fluids from water is disclosed. A tank or bioreactor is provided with an open submersible acoustophoretic separator. The separator captures and holds fluid droplets or particles such as cells, permitting them to coalesce or agglomerate until they are large enough and have sufficient buoyant or weight force to float/sink to the top/bottom of the tank or bioreactor. In a tank or bioreactor, the separator captures and holds particles until they are large enough that their weight causes them to settle out of the host fluid. The acoustophoretic device thus speeds up separation of the particles or droplets from the host fluid.
    Type: Grant
    Filed: August 1, 2017
    Date of Patent: August 11, 2020
    Assignee: FloDesign Sonics, Inc.
    Inventors: Bart Lipkens, Thomas J. Kennedy, III
  • Patent number: 10731196
    Abstract: The disclosure provides recombinant cells and methods for producing a ribosomally synthesized and posttranslationally modified peptide (RiPP), as well as RiPP libraries and methods for producing RiPP libraries.
    Type: Grant
    Filed: October 14, 2016
    Date of Patent: August 4, 2020
    Assignee: UNIVERSITY OF UTAH RESEARCH FOUNDATION
    Inventor: Eric Schmidt
  • Patent number: 10689676
    Abstract: The present invention provides mutant microorganism that have higher lipid productivity than the wild type microorganisms from which they are derived while producing biomass at levels that are at least 45% of wild type biomass productivity under nitrogen replete conditions. Particular mutants produce at least 50% as much FAME lipid as wild type while producing at least the amount of biomass produced by wild type cells under nitrogen replete conditions. Also provided are methods of producing lipid using the mutant strains.
    Type: Grant
    Filed: November 2, 2016
    Date of Patent: June 23, 2020
    Assignee: Synthetic Genomics, Inc.
    Inventors: Imad Ajjawi, Leah Soriaga, Moena Aqui, Eric R. Moellering
  • Patent number: 10041138
    Abstract: A sugar composition comprising: at least 40% dissolved solids in an aqueous solution having a viscosity at least 10% lower than a 42 DE (Dextrose Equivalents) reference solution with a same dissolved solids concentration at a given temperature.
    Type: Grant
    Filed: April 9, 2018
    Date of Patent: August 7, 2018
    Assignee: Virdia, Inc.
    Inventors: Aharon Eyal, Robert Jansen
  • Patent number: 10017795
    Abstract: Methods and systems for simultaneously enhancing the production of both methane and volatile fatty acids in an anaerobic digestion bioreactor are disclosed. In some embodiments, the methods include: providing a stream of organic feedstock; providing a plurality of anaerobic digester bioreactors, each of the plurality of anaerobic digester bioreactors connected in series; step-feeding predetermined percentages of the stream of organic feedstock to two or more of the plurality of anaerobic digester bioreactors; feeding effluent from each of the plurality of anaerobic digester bioreactors to a subsequent one of the plurality of anaerobic digester bioreactors; and anaerobically digesting at least one of the stream of organic feedstock and the effluent from each of the plurality of anaerobic digester bioreactors to develop a final effluent stream including methane and volatile fatty acids. The volatile fatty acids are then microbially converted to lipids in an aerobic bioreactor.
    Type: Grant
    Filed: December 11, 2014
    Date of Patent: July 10, 2018
    Assignee: The Trustees of Columbia University in the City of New York
    Inventor: Kartik Chandran
  • Patent number: 10000402
    Abstract: A method for treating solid organic materials includes providing phagotrophic algae, providing solid organic material, combining the algae and the solid organic material, allowing the algae to grow by engulfing or uptaking the solid organic material, forming an algal product, and collecting the algal product. The method can also include a pretreatment step. The solid organic material can be waste activated sludge. A system for treating and disposing solid organic material is also provided.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: June 19, 2018
    Assignee: The University of Akron
    Inventors: Lu-Kwang Ju, Cong Li, Suo Xiao
  • Patent number: 9976194
    Abstract: A sugar composition comprising at least 40% dissolved solids in an aqueous solution having a viscosity at least 10% lower than a 42 DE (Dextrose Equivalents) reference solution with a same dissolved solids concentration at a given temperature. Another sugar composition comprising at least 30% glucose relative to total sugars, at least 10% mannose relative to total sugars, at least 5% xylose relative to total sugars, and less than 0.25% ash. Another sugar composition comprising at least 30% glucose relative to total sugars at least 10% mannose relative to total sugars, at least 5% xylose relative to total sugars, and at least 2% total furfurals.
    Type: Grant
    Filed: June 3, 2015
    Date of Patent: May 22, 2018
    Assignee: Virdia, Inc.
    Inventors: Aharon Eyal, Robert Jansen
  • Patent number: 9914755
    Abstract: The present invention relates to cyclosporin analogs that are potent inhibitors of cyclophilin D and have low immunosuppressive activity; processes for preparing them; pharmaceutical compositions containing them; and methods for using these analogs and compositions containing them for the treatment of medical conditions, including but not limited to ischemic conditions, such as ischemia-reperfusion (I/R) injury, including myocardial FR injury, cerebral I/R injury, and ocular or retinal I/R injury.
    Type: Grant
    Filed: January 8, 2016
    Date of Patent: March 13, 2018
    Assignee: Allergan, Inc.
    Inventors: Simon N. Pettit, Andrew D. Jones, Catherine Simone V. Frydrych, Alex J. Thomas, Michael E. Garst
  • Patent number: 9873859
    Abstract: Herein disclosed is a method of generating products from microorganisms, comprising super-saturating a liquid medium with a gas consumable by the microorganisms in a high shear device operating at a shear rate of greater than 1,000,000 s?1 to produce a gas-super-saturated (GSS) medium, wherein the GSS medium maintains a GSS level for at least 10 minutes; feeding the GSS medium to microorganisms; allowing the microorganisms to grow by consuming the gas and generate products via photosynthesis or chemosynthesis; and recovering the products. In an embodiment, the microorganisms are genetically modified. In an embodiment, the microorganisms include bacteria, protozoa, algae, or fungi, or a combination thereof. In an embodiment, the gas consumable by the microorganisms is selected from the group consisting of carbon dioxide, nitrogen, air, oxygen, methane, and combinations thereof. A suitable system is also discussed in this disclosure.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: January 23, 2018
    Assignee: H R D Corporation
    Inventors: Abbas Hassan, Aziz Hassan, Gregory G. Borsinger, Rayford Gaines Anthony
  • Patent number: 9862910
    Abstract: Herein disclosed is a method of processing a medium containing algae microorganisms to produce algal oil and by-products, comprising providing the medium containing algae microorganisms; passing the medium through a rotor-stator high shear device; disintegrating cell walls of and intracellular organelles in the algae microorganisms to release algal oil and by-products; and removing the algae medium from an outlet of the high shear device. In an embodiment, disintegration is enhanced by a penetrating gas capable of permeating the cell wall. In an embodiment, enhancement is accomplished by super-saturation of the penetrating gas in the medium or increased gas pressure in a vessel. In an embodiment, the penetrating gas is different from the gas produced by the cell during respiration. A suitable system is also discussed in this disclosure.
    Type: Grant
    Filed: August 20, 2015
    Date of Patent: January 9, 2018
    Assignee: H R D Corporation
    Inventors: Abbas Hassan, Aziz Hassan, Gregory G. Borsinger, Rayford Gaines Anthony
  • Patent number: 9796956
    Abstract: An acoustophoresis device made up of modular components is disclosed. Several modules are disclosed herein, including ultrasonic transducer modules, input/output modules, collection well modules, and various connector modules. These permit different systems to be constructed that have appropriate fluid dynamics for separation of particles, such as biological cells, from a fluid.
    Type: Grant
    Filed: October 3, 2016
    Date of Patent: October 24, 2017
    Assignee: FloDesign Sonics, Inc.
    Inventors: Bart Lipkens, Thomas J. Kennedy, III, Jeffrey King, Jason Barnes, Brian McCarthy, Dane Mealey, Erik Miller, Walter M. Presz, Jr., Benjamin Ross-Johnsrud, John Rozembersky
  • Patent number: 9745569
    Abstract: Acoustophoretic devices and methods for concentrating targeted biological cells in a reduced volume using multi-dimensional acoustic standing waves are disclosed. The methods include flowing a mixture of a host fluid and the biological cells through an acoustophoretic device. The acoustophoretic devices include an inlet, an outlet, and a flow chamber having an ultrasonic transducer-reflector pair. Biological cells, such as T cells, are separated from a host fluid for utilization in allergenic or autologous cell therapies. The disclosed devices and methods are capable of concentrating biological cells to at least 100 times their original cell concentration. The disclosed methods and devices are further capable of decreasing an original feed volume to a final concentrated volume that is less than one percent of the original feed volume.
    Type: Grant
    Filed: January 26, 2017
    Date of Patent: August 29, 2017
    Assignee: FloDesign Sonics, Inc.
    Inventors: Bart Lipkens, Jason Dionne, Goutam Ghoshal
  • Patent number: 9725705
    Abstract: Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium.
    Type: Grant
    Filed: December 20, 2013
    Date of Patent: August 8, 2017
    Assignee: Allergan, Inc.
    Inventor: Hui Xiang
  • Patent number: 9364492
    Abstract: The present invention provides a method for preparing a glycoside of a flavonoid compound, which comprises the step of treating flavonoid and a glycosyl donor with an enzymatic agent having glycosylation activity and being derived from the genus Trichoderma (preferably Trichoderma viride or Trichoderma reesei). Such a flavonoid compound includes a catechin compound or a methylated derivative thereof, and the glycosyl donor includes a carbohydrate containing a maltotriose residue (preferably maltotriose, maltotetraose, maltopentaose, maltohexaose, maltoheptaose, dextrin, ?-cyclodextrin or soluble starch). Glycosides obtained by the present invention have higher water solubility, improved taste, and increased stability. The present invention also provides novel glycosides of catechin compounds, which are obtained by the method of the present invention.
    Type: Grant
    Filed: January 18, 2008
    Date of Patent: June 14, 2016
    Assignee: SUNTORY HOLDINGS LIMITED
    Inventors: Misa Ochiai, Harukazu Fukami, Masahiro Nakao, Akio Noguchi
  • Patent number: 9340813
    Abstract: The present specification discloses expression constructs comprising single-chain proteins comprising a di-chain loop region comprising an exogenous protease cleavage site and a protease that can cleave the exogenous protease cleavage site located within the di-chain loop, cell compositions comprising such expression construct, and intracellular methods of converting the single-chain protein into its di-chain form.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: May 17, 2016
    Assignee: Allergan, Inc.
    Inventors: Sanjiv Ghanshani, Linh Q. Le, Yi Liu, Lance E. Steward
  • Patent number: 9315833
    Abstract: The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.
    Type: Grant
    Filed: February 18, 2010
    Date of Patent: April 19, 2016
    Assignees: Lallemand Hungary Liquidity Management LLC, Stellenbosch University
    Inventors: John McBride, Mark Mellon, Vineet Rajgarhia, Elena E. Brevnova, Erin Wiswall, David A. Hogsett, Danie LaGrange, Shaunita Rose, Emile Van Zyl
  • Patent number: 9279000
    Abstract: Methods for producing proteins, for example, recombinant meningococcal 2086 proteins, using fed-batch fermentation with continuous input of an inducer after achieving a threshold parameter, and optionally continuous input of a carbon source, for example, a constant rate input, to improve protein yields, as well as high density protein compositions and compositions for use in the methods of the present invention, are provided.
    Type: Grant
    Filed: December 19, 2013
    Date of Patent: March 8, 2016
    Assignee: Wyeth LLC
    Inventors: Wei-Qiang Willie Sun, Earl Joseph Pursell
  • Patent number: 9272035
    Abstract: Methods of treating, preventing, or managing autoimmune inflammatory diseases and disorders including but not limited to spondylitis, juvenile rheumatoid arthritis, psoriasis, psoriatic arthritis, osteoarthritis, ankylosing spondylitis, and rheumatoid arthritis by the administration of phosphodiesterase 4 (PDE4) inhibitors in combination with other therapeutics are disclosed. Pharmaceutical compositions, dosage forms, and kits suitable for use in methods of the invention are also disclosed.
    Type: Grant
    Filed: April 27, 2012
    Date of Patent: March 1, 2016
    Assignee: Celgene Corporation
    Inventors: Peter H. Schafer, Anita Gandhi, Lori Capone
  • Patent number: 9243253
    Abstract: Provided herein are methods and compositions for expression of a nucleic acid construct comprising nucleic acids encoding a) a recombinant polypeptide, and b) a prototrophy-restoring enzyme in a host cell that is auxotrophic for at least one metabolite. In various embodiments, the host cell is auxotrophic for a nitrogenous base compound or an amino acid. The invention involves introducing an analogue into the growth media for the host cell such that the analogue is incorporated into the recombinant polypeptide or a nucleic acid coding sequence thereof. In various embodiments, the compositions and methods disclosed herein result in improved recombinant protein expression compared to expression of recombinant protein in an antibiotic selection system, or compared to expression of the recombinant protein in an expression system that lacks a metabolite analogue.
    Type: Grant
    Filed: May 10, 2010
    Date of Patent: January 26, 2016
    Assignee: Pfenex, Inc.
    Inventors: Diane M. Retallack, Lawrence C. Chew, Charles H. Squires
  • Patent number: 9040294
    Abstract: According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein.
    Type: Grant
    Filed: August 24, 2012
    Date of Patent: May 26, 2015
    Assignees: KABUSHIKI KAISHA TOSHIBA, Toshiba Medical Systems Corporation
    Inventors: Eiichi Akahoshi, Mitsuko Ishihara
  • Patent number: 9040258
    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).
    Type: Grant
    Filed: June 28, 2011
    Date of Patent: May 26, 2015
    Assignee: Dyax Corp.
    Inventors: Simon E. Hufton, Hendricus Renerus Jacobus Mattheus Hoogenboom
  • Patent number: 9034601
    Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).
    Type: Grant
    Filed: July 9, 2013
    Date of Patent: May 19, 2015
    Assignee: Dyax Corp.
    Inventors: Simon E. Hufton, Hendricus Renerus Jacobus Mattheus Hoogenboom
  • Patent number: 9029105
    Abstract: The present invention relates to a method for producing L-methionine, comprising: i) culturing an L-methionine precursor-producing microorganism strain in a fermentation solution, so that the L-methionine precursor accumulates in the solution; and ii) mixing a converting enzyme and methylmercaptan or its salts with at least a portion of the solution to convert the accumulated L-methionine precursor into L-methionine, as well as to microorganism strains used in each step.
    Type: Grant
    Filed: January 29, 2013
    Date of Patent: May 12, 2015
    Assignee: CJ Cheiljedang Corporation
    Inventors: So-young Kim, Kwang-myung Choi, Yong-uk Shin, Hye-won Um, Kyung-oh Choi, Jin-sook Chang, Young-wook Cho, Young-hoon Park
  • Patent number: 9029126
    Abstract: A method of hydrothermally treating stillage by heating stillage to 200 degrees F. to 350 degrees F., altering physicochemical properties of the stillage, enabling facile separation of the stillage, and creating unique product fractions. A method of performing ethanol fermentation by treating stillage to enable facile separation by heating the stillage to a temperature of 200 degrees F. to 350 degrees F., and separating the treated stillage to recover a high protein solids fraction, a stickwater fraction, and an oil fraction. A method of improving fermentation by heating stillage to a temperature of 200° F. to 350° F. resulting in hydrothermally treated stillage, using all or a portion of the hydrothermally treated stillage as a component of a media, and using the media for a process including fermentation and biomass production. Oil, stickwater, high protein solids fraction, high protein meal, metabolites, biomass, and media obtained from the methods above.
    Type: Grant
    Filed: June 20, 2013
    Date of Patent: May 12, 2015
    Assignee: Valicor, Inc.
    Inventors: James Robert Bleyer, Thomas J Czartoski, Puneet Chandra
  • Publication number: 20150125906
    Abstract: The present invention relates to methods of degrading or converting biomass material enriched with hemicellulosic material into fermentable sugars.
    Type: Application
    Filed: January 14, 2015
    Publication date: May 7, 2015
    Inventors: Prashant Iyer, Harry Showmaker, Hui Xu, Kishore Rane
  • Patent number: 9023618
    Abstract: The present invention relates to a recombinant E. coli exhibiting a complex phenotype, comprising one or more RNA polymerase subunit genes, one or more functional genes, and, optionally, one or more transcription factors from a heterologous prokaryote. Also provided are methods for screening such a recombinant E. coli.
    Type: Grant
    Filed: June 2, 2011
    Date of Patent: May 5, 2015
    Assignee: Eleftherios Papoutsakis and Stefan Gaida
    Inventors: Eleftherios T. Papoutsakis, Stefan Marcus Gaida
  • Patent number: 9023989
    Abstract: Protein-based photovoltaic cells and the manufacture and use of protein-based photovoltaic cells are described. In one embodiment, bacteriorhodopsin from Halobacterium salinarum, which undergoes structural transitions when irradiated with a given wavelength of light, is used as the protein in the protein-based photovoltaic cells. In another embodiment, mutant bacteriorhodopsin from H. salinarum is used. Exposure of the protein to sunlight causes proton transfer across a membrane resulting in the generation of an electrical charge. The protein can be oriented and/or layered on a substrate and modified by mutation to enhance transmembrane proton transfer, covalent binding to a substrate and layering. The protein-based photovoltaic cells sequentially or simultaneously generate hydrogen gas from water or salt, which also can be harnessed to produce electricity.
    Type: Grant
    Filed: February 4, 2009
    Date of Patent: May 5, 2015
    Assignee: University of Connecticut
    Inventors: Robert R. Birge, Rekha Rangarajan
  • Patent number: 9017969
    Abstract: The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.
    Type: Grant
    Filed: March 1, 2012
    Date of Patent: April 28, 2015
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Hiroko Abe, Kazuya Tomimoto, Yasuko Fujita, Tomoko Iwaki, Yasunori Chiba, Yoshihiro Nakajima, Kenichi Nakayama, Masatoshi Kataoka, Shouki Yatsushiro, Shohei Yamamura
  • Publication number: 20150111253
    Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.
    Type: Application
    Filed: March 12, 2013
    Publication date: April 23, 2015
    Applicant: EVONIK INDUSTRIES AG
    Inventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
  • Publication number: 20150107318
    Abstract: The method contains the following steps. In step A, supersonic waves are applied to Mountain Litsea fruits for excitation and sterilization. In step B, the inferior fruits and the cleaning water are removed from the water tank, placed in a fermentative tank, and left for fermentation. After the fermentation, a pressing and separation process is conducted. In step C, the superior fruits are placed in a separate fermentative tank for fermentation into mofetil alcohol. In step D, the superior fruits are alternatively mixed with pure water, crushed, and placed in a boiler for aging and sterilization. Then the crushed and aged fruits are placed in yet another fermentative tank with pure water for fermentation. After the fermentation, a pomace separation process is conducted. The separated pomace becomes a Mountain Litsea wine lees, whereas the separated water becomes a Mountain Litsea enzymatic liquid.
    Type: Application
    Filed: October 22, 2013
    Publication date: April 23, 2015
    Inventors: HENG-JUI HSU, TSU-YU HSU
  • Patent number: 9012226
    Abstract: The present invention relates to the propagation of covalently closed circular recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly is strain modifications that improve strain viability, plasmid stability, plasmid production yield, and plasmid-directed protein production yield, using said DNA molecules in fermentation culture.
    Type: Grant
    Filed: March 10, 2010
    Date of Patent: April 21, 2015
    Assignee: Nature Technology Corporation
    Inventor: James A. Williams