Using A Micro-organism To Make A Protein Or Polypeptide Patents (Class 435/71.1)
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Patent number: 9017969Abstract: The present invention provides: genetically modified yeasts such as mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and a decreased ability to produce O-linked sugar chains, mutant yeasts having an ability to produce N-linked sugar chains of Man5GlcNAc2 and further having an ability to produce N-linked sugar chains of GlcNAc1Man5GlcNAc2, and mutant yeasts having an increased ability to produce and secrete proteins and an ability to produce N-linked sugar chains of Man5GlcNAc2; and a method for producing glycoproteins using them.Type: GrantFiled: March 1, 2012Date of Patent: April 28, 2015Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Hiroko Abe, Kazuya Tomimoto, Yasuko Fujita, Tomoko Iwaki, Yasunori Chiba, Yoshihiro Nakajima, Kenichi Nakayama, Masatoshi Kataoka, Shouki Yatsushiro, Shohei Yamamura
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Publication number: 20150111253Abstract: The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof.Type: ApplicationFiled: March 12, 2013Publication date: April 23, 2015Applicant: EVONIK INDUSTRIES AGInventors: Steffen Schaffer, Michaela Hauberg, Mirja Wessel, Hans-Georg Hennemann, Jan Christoph Pfeffer, Thomas Haas, Harald Haeger
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Publication number: 20150107318Abstract: The method contains the following steps. In step A, supersonic waves are applied to Mountain Litsea fruits for excitation and sterilization. In step B, the inferior fruits and the cleaning water are removed from the water tank, placed in a fermentative tank, and left for fermentation. After the fermentation, a pressing and separation process is conducted. In step C, the superior fruits are placed in a separate fermentative tank for fermentation into mofetil alcohol. In step D, the superior fruits are alternatively mixed with pure water, crushed, and placed in a boiler for aging and sterilization. Then the crushed and aged fruits are placed in yet another fermentative tank with pure water for fermentation. After the fermentation, a pomace separation process is conducted. The separated pomace becomes a Mountain Litsea wine lees, whereas the separated water becomes a Mountain Litsea enzymatic liquid.Type: ApplicationFiled: October 22, 2013Publication date: April 23, 2015Inventors: HENG-JUI HSU, TSU-YU HSU
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Patent number: 9012226Abstract: The present invention relates to the propagation of covalently closed circular recombinant DNA molecules such as plasmids, cosmids, bacterial artificial chromosomes (BACs), bacteriophages, viral vectors and hybrids thereof, and more particularly is strain modifications that improve strain viability, plasmid stability, plasmid production yield, and plasmid-directed protein production yield, using said DNA molecules in fermentation culture.Type: GrantFiled: March 10, 2010Date of Patent: April 21, 2015Assignee: Nature Technology CorporationInventor: James A. Williams
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Patent number: 9012181Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).Type: GrantFiled: November 24, 2009Date of Patent: April 21, 2015Assignee: Dyax Corp.Inventors: Simon E. Hufton, Hendricus R. J. M. Hoogenboom
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Patent number: 9012177Abstract: The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell.Type: GrantFiled: May 28, 2014Date of Patent: April 21, 2015Assignees: The Regents of the University of California, The Board of Trustees of the University of Illinois, BP Corporation North America Inc.Inventors: N. Louise Glass, Chaoguang Tian, William T. Beeson, IV, Huimin Zhao, Jing Du, Jin Ho Choi, James H. Doudna Cate, Jonathan M. Galazka, Suk-Jin Ha, Yong-Su Jin, Soo Rin Kim, Sijin Li, Xiaomin Yang
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Patent number: 9012192Abstract: A method of increasing the rate of growth, useful product production, or protein expression of a microorganism includes the step of exposing the microorganism to ultrasound having a frequency greater than about 1 MHz.Type: GrantFiled: September 21, 2011Date of Patent: April 21, 2015Assignee: Intelligentnano Inc.Inventors: Jie Chen, James Xing, Woon T. Ang
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Patent number: 9005947Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl7, and the corresponding BGL7 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL7, recombinant BGL7 proteins and methods for producing the same.Type: GrantFiled: January 17, 2014Date of Patent: April 14, 2015Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Michael Ward
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Patent number: 9005948Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.Type: GrantFiled: January 20, 2014Date of Patent: April 14, 2015Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Patent number: 9005927Abstract: A eukaryotic expression vector capable of displaying a multi-chain polypeptide on the surface of a host cell is provided, such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the host cell. Such a vector allows for the display of complex biologically active polypeptides, e.g., biologically active multi-chain polypeptides such as immunoglobulin Fab fragments. The present invention describes and enables the successful display of a multi-chain polypeptide on the surface of a eukaryotic host cell. Preferred vectors are described for expressing the chains of a multi-chain polypeptide in a host cell separately and independently (e.g., under separate vector control elements, and/or on separate expression vectors, thus forming a matched vector set).Type: GrantFiled: March 28, 2011Date of Patent: April 14, 2015Assignee: Dyax Corp.Inventors: Simon E. Hufton, Hendricus R. J. M. Hoogenboom
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Patent number: 8999691Abstract: Disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in Escherichia coli and dramatically increased thermal stability, which is obtained by introducing specific amino acid mutations to glucose dehydrogenase derived from Botryotinia fuckeliana. Also disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in E. coli and dramatically increased thermal stability, which is obtained by replacing two amino acid residues in glucose dehydrogenase of fungal origin with cysteine residues. The novel glucose dehydrogenase has a low reactivity to xylose.Type: GrantFiled: June 29, 2011Date of Patent: April 7, 2015Assignee: Ultizyme International Ltd.Inventors: Koji Sode, Kazushige Mori
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Publication number: 20150093785Abstract: The invention provides apparatus and processes for cultivating algae.Type: ApplicationFiled: December 8, 2014Publication date: April 2, 2015Inventors: Ari KETOLA, Veikko LATVALA
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Patent number: 8993267Abstract: The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprising lignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.Type: GrantFiled: December 17, 2007Date of Patent: March 31, 2015Assignee: Danisco US Inc.Inventors: Elizabeth A Bodie, George England
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Patent number: 8993268Abstract: Provided are a method of producing Clostridium botulinum toxin by using a media containing plant-derived components, and a method of producing Clostridium botulinum toxin by using a flexible closed container.Type: GrantFiled: July 2, 2008Date of Patent: March 31, 2015Assignee: Medy-Tox Inc.Inventors: Hyun-Ho Jung, Gi-Hyeok Yang, Hack-Woo Kim, Byung-Kook Lee, Young-suk Yoon, Hyung-Pyo Hong
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Patent number: 8993299Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: GrantFiled: October 28, 2013Date of Patent: March 31, 2015Assignee: Novozymes A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Patent number: 8993296Abstract: An object of the present invention is to provide enzymes associated with equol synthesis, genes coding such enzymes, and a process for producing equol and its intermediates using the enzymes and genes. The present invention provides a dihydrodaidzein synthesizing enzyme, tetrahydrodaidzein synthesizing enzyme, equol synthesizing enzyme, and genes coding these enzymes. The present invention also provides a process for synthesizing dihydrodaidzein, tetrahydrodaidzein, and/or equol using these enzymes.Type: GrantFiled: January 15, 2014Date of Patent: March 31, 2015Assignee: Otsuka Pharmaceutical Co., Ltd.Inventors: Yoshikazu Shimada, Setsuko Yasuda, Masayuki Takahashi, Takashi Hayashi, Norihiro Miyazawa, Yasuhiro Abiru, Tadaaki Ohtani, Ikutaro Sato
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Patent number: 8993743Abstract: The present invention relates to a nucleic acid molecule encoding a chimeric protein having the biochemical activity of a surface active protein, wherein said chimeric protein comprises: (a) an N-terminal portion of a first surface active protein, wherein the N-terminal portion is devoid of between 0 and 10 of the most N-terminal amino acids of the mature first surface active protein; and, C-terminally thereof, (b) a C-terminal portion of a second surface active protein, wherein the C-terminal portion is devoid of between 0 and 10 of the most C-terminal amino acids of the mature second surface active protein. The present invention further relates to a vector, a non-human host and a method for the production of a chimeric protein having the biochemical activity of a surface active protein. In addition, the present invention relates to a chimeric protein encoded by the nucleic acid molecule of the invention and a composition comprising the chimeric protein.Type: GrantFiled: February 18, 2011Date of Patent: March 31, 2015Assignee: B.R.A.I.N. Biotechnology Research and Information Network AGInventors: Guido Meurer, Esther Gabor, Anke Bachert, Jürgen Eck
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Patent number: 8986987Abstract: The present application applies to the field of veterinary vaccines, in particular of vaccines for poultry against avian influenza. The vaccine is based on a recombinant viral vector expressing the haemagglutinin protein of an influenza virus, wherein the vector is herpes virus of turkeys (HVT) and the haemagglutinin gene is driven by a glycoprotein B gene promoter from a mammalian herpesvirus. A vaccine comprising this HVT+HA vector can be used to induce a protective immune response against avian influenza in poultry, and to reduce the spread of AIV. The invention also relates to methods, uses, and vaccines involving the HVT+HA vector.Type: GrantFiled: October 17, 2011Date of Patent: March 24, 2015Assignee: Intervet Inc.Inventors: Paulus Jacobus Antonius Sondermeijer, Iwan Verstegen
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Patent number: 8986969Abstract: The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.Type: GrantFiled: October 28, 2013Date of Patent: March 24, 2015Assignee: Novozymes A/SInventors: Lene Lange, Wenping Wu, Dominique Aubert, Sara Landvik, Kirk Matthew Schnorr, Ib Groth Clausen
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Patent number: 8986977Abstract: Presented herein are methods of using cell wall degrading enzymes for recovery of internal lipid bodies from biomass sources such as algae. Also provided are algal cells that express at least one exogenous gene encoding a cell wall degrading enzyme and methods for recovering lipids from the cells.Type: GrantFiled: December 31, 2012Date of Patent: March 24, 2015Assignee: Alliance for Sustainable Energy, LLCInventors: Eric P. Knoshaug, Bryon S. Donohoe, Henri Gerken, Lieve Laurens, Stefanie Rose Van Wychen
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Patent number: 8986706Abstract: The present invention encompasses recombinant Newcastle Disease Virus-Herpesvirus vaccines or compositions. The invention encompasses recombinant NDV vectors encoding and expressing herpesvirus pathogen, antigens, proteins, epitopes or immunogens. Such vaccines or compositions can be used to protect animals against disease.Type: GrantFiled: August 29, 2011Date of Patent: March 24, 2015Assignee: Merial, Inc.Inventors: Michel Bublot, Frederic Reynard, Herve Poulet, Frederic Raymond David
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Patent number: 8980605Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: March 11, 2013Date of Patent: March 17, 2015Assignee: Codexis, Inc.Inventors: Charlene Ching, John M. Gruber, Gjalt W. Huisman, Emily Mundorff, Lisa M. Newman
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Patent number: 8969066Abstract: The present invention provides compositions and methods for producing flocculation moieties in photosynthetic organisms. The photosynthetic organisms are genetically modified to effect production, secretion, or both, of the flocculation moieties. Also provided are methods of flocculating organisms.Type: GrantFiled: June 26, 2009Date of Patent: March 3, 2015Assignee: Sapphire Energy, Inc.Inventors: Michael Mendez, Craig Behnke, Yan Poon, Philip Lee
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Patent number: 8961995Abstract: The present invention provides alphavirus replicons and methods of their use in producing heterologous protein.Type: GrantFiled: March 7, 2013Date of Patent: February 24, 2015Assignee: UAB Research FoundationInventors: Ilya Frolov, Elena Frolova
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Patent number: 8956846Abstract: The present invention relates to a method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated by an oscillation of the dissolved oxygen partial pressure in the medium.Type: GrantFiled: March 24, 2011Date of Patent: February 17, 2015Assignee: IFP Energies nouvellesInventors: Fadhel Ben Chaabane, Celine Cohen
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Patent number: 8957194Abstract: Antagonists of human proprotein convertase subtilisin-kexin type 9 (“PCSK9”) are disclosed. The disclosed antagonists are effective in the inhibition of PCSK9 function and, accordingly, present desirable antagonists for use in the treatment of conditions associated with PCSK9 activity. The present invention also discloses nucleic acid encoding said antagonists, vectors, host cells, and compositions comprising the antagonists. Methods of making PCSK9-specific antagonists as well as methods of using the antagonists for inhibiting or antagonizing PCSK9 function are also disclosed and form important additional aspects of the present disclosure.Type: GrantFiled: May 8, 2012Date of Patent: February 17, 2015Assignee: Merck Sharpe & Dohme Corp.Inventors: Jon H. Condra, Rose M. Cubbon, Holly A. Hammond, Timothy McCabe, Shilpa Pandit, Laurence B. Peterson, Joseph C. Santoro, Ayesha Sitlani, Dana D. Wood, Henryk Mach, Heidi Pixley, Sonia M. Gregory, Jeffrey T. Blue, Kevin Wang, Peizhi (Peter) Luo, Denise K. Nawrocki, Pingyu Zhong, Feng Dong, Yan Li
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Publication number: 20150044356Abstract: The present invention describes a bio-based process to produce high quality protein concentrate (HQPC) and lipids by converting plant derived materials into bioavailable protein and lipids via solid state fermentation (SSF) and hybrid-SSF, including the use of such HQPC and lipids so produced as nutrients, including use as a fish meal replacement in aquaculture diets. Also disclosed is a SSF reactor and method of using the reactor.Type: ApplicationFiled: August 6, 2014Publication date: February 12, 2015Applicant: PRAIRIE AQUATECHInventors: Jason A. Bootsma, William R. Gibbons, Michael L. Brown
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Patent number: 8945864Abstract: The object of the present invention is to provide a method of determining 1,5-anhydroglucitol, including using (a) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (b) a protein which consists of an amino acid sequence having deletion, substitution and/or addition of one or more amino acid residues in the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity; or (c) a protein which consists of an amino acid sequence having a homology of at least 60% with the amino acid sequence of SEQ ID NO: 2 and which has sorbose dehydrogenase activity.Type: GrantFiled: June 22, 2007Date of Patent: February 3, 2015Assignees: Ikeda Food Research Co., Ltd., Nippon Kayaku Kabushiki KaishaInventors: Emi Ishimaru, Hirokazu Sanada, Hironori Omura, Hideki Yoshioka, Shuhei Tsukamoto, Minoru Masuda
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Patent number: 8945899Abstract: Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.Type: GrantFiled: December 15, 2009Date of Patent: February 3, 2015Assignee: Butamax Advanced Biofuels LLCInventors: Yougen Li, Der-Ing Liao, Mark J. Nelson, Daniel P. Okeefe
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Patent number: 8945879Abstract: The present invention relates to isolated polypeptides having protease activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.Type: GrantFiled: April 1, 2014Date of Patent: February 3, 2015Assignee: Novozymes A/SInventors: Hiroaki Udagawa, Christian Isak Jorgensen
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Patent number: 8945530Abstract: The present invention relates to the provision of a DNA sequence of the major grass pollen allergen Lol p 4. The invention also encompasses fragments, new combinations of partial sequences and point mutants having a hypoallergenic action. The recombinant DNA molecules and the derived polypeptides, fragments, new combinations of partial sequences and variants can be utilised for the therapy of pollen-allergic diseases. The proteins prepared by recombinant methods can be employed for in vitro and in vivo diagnosis of pollen allergies.Type: GrantFiled: December 6, 2010Date of Patent: February 3, 2015Assignee: Merck Patent GmbHInventors: Andreas Nandy, Helmut Fiebig, Oliver Cromwell
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Patent number: 8932576Abstract: Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.Type: GrantFiled: January 27, 2010Date of Patent: January 13, 2015Assignee: The Scripps Research InstituteInventor: Stephen P. Mayfield
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Patent number: 8932829Abstract: Disclosed are pharmaceutical and synergistic compositions comprising human recombinant alpha-fetoprotein expressed in eucaryotic cells for preparation of therapeutic agents for use in oncology, immunotherapy, stem cell therapy and cosmetology and also for the diagnosis of cancer and embryonic pathologies.Type: GrantFiled: October 22, 2010Date of Patent: January 13, 2015Inventors: Elena Dudich, Lydia Semenkova, Igor Dudich, Eduard Tatulov
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Patent number: 8932827Abstract: Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.Type: GrantFiled: February 28, 2012Date of Patent: January 13, 2015Assignee: Allergan, Inc.Inventors: Jennifer L. Ton, Hemant A. Patel, Ronald C. Bates, Wajdie M. Ahmad
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Patent number: 8927256Abstract: The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.Type: GrantFiled: February 5, 2009Date of Patent: January 6, 2015Assignee: Biocon LimitedInventors: Sanjay Tiwari, Mukesh Babuappa Patale, Saurabh Garg, Mayank Kumar Garg, Sulekha Joshi, Chittnalli Ramegowda Naveen Kumar, Bimal Kumar, Anuj Goel, Harish Iyer
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Patent number: 8927231Abstract: Provided is a separatome-based recombinant peptide, polypeptide, and protein expression and purification platform based on the juxtaposition of the binding properties of host cell genomic peptides, polypeptides, and proteins with the characteristics and location of the corresponding genes on the host cell chromosome, such as that of E. coli, yeast, Bacillus subtilis or other prokaryotes, insect cells, mammalian cells, etc. This platform quantitatively describes and identifies priority deletions, modifications, or inhibitions of certain gene products to increase chromatographic separation efficiency, defined as an increase in column capacity, column selectivity, or both, with emphasis on the former. Moreover, the platform provides a computerized knowledge tool that, given separatome data and a target recombinant peptide, polypeptide, or protein, intuitively suggests strategies leading to efficient product purification.Type: GrantFiled: October 17, 2013Date of Patent: January 6, 2015Assignees: The Board of Trustees of the University of Arkansas, University of Pittsburgh—Of The Commonwealth System of Higher EducationInventors: Ellen M. Brune, Robert R. Beitle, Jr., Mohammad M. Ataai, Patrick R. Bartlow, Ralph L. Henry
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Patent number: 8927229Abstract: Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.Type: GrantFiled: January 5, 2012Date of Patent: January 6, 2015Assignee: Allergan, Inc.Inventors: Jennifer L. Ton, Hemant A. Patel, Ronald C. Bates, Wajdie M. Ahmad
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Patent number: 8916748Abstract: This invention relates generally to nucleic acid sequences encoding proteins that are related to the presence of seed storage compounds in plants. More specifically, the present invention relates to Arabidopsis thaliana, Brassica napus, Glycine max and Oryza sativa nucleic acid sequences encoding sugar and lipid metabolism regulator proteins and the use of these sequences in transgenic plants. In particular, the invention is directed to methods for manipulating sugar-related compounds and for increasing oil level and altering the fatty acid composition in plants and seeds. The invention further relates to methods of using these novel plant polypeptides to stimulate plant growth and/or to increase yield and/or composition of seed storage compounds.Type: GrantFiled: December 4, 2006Date of Patent: December 23, 2014Assignee: BASF Plant Science GmbHInventors: Heiko A. Haertel, Garima Bhatt
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Patent number: 8911968Abstract: High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.Type: GrantFiled: December 15, 2011Date of Patent: December 16, 2014Assignee: Shanghai Techwell Biopharmaceutical Co., Ltd.Inventors: Yi Chen, Shidong Liu, Zhaoli Zhang, Chunxia Wang, Jing Kang, Xiaoming Ji
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Patent number: 8906663Abstract: The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.Type: GrantFiled: November 16, 2012Date of Patent: December 9, 2014Assignee: Verenium CorporationInventors: David Weiner, Ellen Chi, Jennifer Ann Chaplin, Aileen Milan, Jay M. Short, Grace DeSantis, Mark Madden, Mark J. Burk, Dan E. Robertson
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Patent number: 8906650Abstract: Promoter regions associated with the Yarrowia lipolytica esterase/lipase (EL1) gene are disclosed and have been found to be particularly effective for the expression of heterologous genes in yeast. These promoter regions will be useful for driving high-level expression of genes involved in the production of omega-3 and omega-6 fatty acids.Type: GrantFiled: March 30, 2012Date of Patent: December 9, 2014Assignee: E I du Pont de Nemours and CompanyInventor: Quinn Qun Zhu
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Patent number: 8901374Abstract: The invention provides isolated nucleic acid molecules which encode novel fatty acid dehydratase family members. The invention also provides recombinant expression vectors containing dehydratase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., SDA, EPA and DHA.Type: GrantFiled: June 2, 2009Date of Patent: December 2, 2014Assignees: BASF Plant Science GmbH, Bioriginal Food & Science Corp.Inventors: Jörg Bauer, Xiao Qiu, Patricia Vrinten
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Publication number: 20140349345Abstract: The present invention relates to a method for the fermentation of cyclopeptides such as pneumocandins in the presence of increased concentrations of any or all of calcium, copper, iron, magnesium, manganese, molybdenum and zinc ions.Type: ApplicationFiled: January 7, 2013Publication date: November 27, 2014Inventors: Wouter Adrianus Van Winden, Rudolf Van Der Pol
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Patent number: 8889406Abstract: An apparatus for cultivating cells utilizing wave motion comprising a container, a retaining member configured to retain the container, a drive assembly for swiveling the container with respect to the substantially horizontal pivot axis and to swivel, such that during swiveling the pivot axis follows a cyclical closed-loop path.Type: GrantFiled: December 27, 2007Date of Patent: November 18, 2014Assignee: Strix Beheer B.V.Inventors: Pieter van der Heiden, Marc Buevink, Nicolaas Marius Gerard Oosterhuis
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Publication number: 20140335566Abstract: Embodiments described herein relate to mobile biologics production systems that can be transported to a site where a production of biologics is required, and is operable to produce the biologics on-site on demand. A mobile biologics production system can include a mobile carrier configured to be trailered to a biologics production location. The mobile carrier defines an interior volume configured to provide an aseptic environment for manufacturing, production, and purification of biologics. A production sub-system disposed in the interior volume includes a single-use bioreactor configured to grow biologics which can include microbes, mammalian cell lines, insect cell lines, and plant cell lines. The bioreactor is controlled by a controller, and can have a working capacity of less than about 100 L. A purification sub-system disposed in the internal volume is fluidically coupled to the production sub-system and configured to produce a finished biologic product.Type: ApplicationFiled: September 5, 2013Publication date: November 13, 2014Applicant: BioCapacity On Demand, LLCInventors: Michael Nicholas Guerini, Robert O. Keene
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Patent number: 8882484Abstract: The method of the invention provides for producing a heterologous protein in mammalian host cells having nucleic acid encoding Hepatitis B X protein and the heterologous protein, by growing mammalian host cells selected from the group consisting of HKB11, CHO, BHK21, C2C12, and HEK293 cells, by growing mammalian host cells in non-adherent suspension culture, or by growing mammalian host cells which contain nucleic acid providing exogenous X-box Binding Protein, XBP1s. The conditions should be such that HBx, exogenous XBP1s if present, and the heterologous protein are expressed by the mammalian cells. The invention includes compositions for carrying out the method.Type: GrantFiled: May 27, 2009Date of Patent: November 11, 2014Assignee: Bayer HealthCare LLCInventors: Fang Jin, Richard N. Harkins, Maxine Bauzon, Terry Hermiston
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Patent number: 8883450Abstract: A use of a signal peptide for producing a recombinant polypeptide of interest in an expression system, the signal peptide includes at least 12 amino acids of formula (I): (X1)iX2X3X4SX5X6X7, wherein: X1 is a peptide containing from 3 to 6 amino acids, i equal to 0 or 1, X2 is a peptide containing from 3 to 9 hydrophobic amino acids, X3 is a peptide containing from 3 to 5 amino acids, the peptide including at least 3 contiguous or non-contiguous leucines X4 is a peptide containing from 2 to 5 amino acids chosen from Ala, Thr, Ser, Gln, Ile, Met, X5 is Ala or Val, X6 is Gln, Asn or His, X7 is Ala or Cys, provided that when the signal peptide originates from a natural precursor of a specific protein, the polypeptide of interest is different from the protein.Type: GrantFiled: March 17, 2011Date of Patent: November 11, 2014Assignee: Laboratoire Francais du Fractionnement et des BiotechnologiesInventor: Alexandre Fontayne
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Patent number: 8883993Abstract: The present invention provides novel promoter and terminator sequences for use in gene expression in eukaryotic cells, such as algal cells. The invention further provides expression cassettes comprising a promoter, as described herein, operably linked to a gene. The invention further provides expression vectors and host eukaryotic cells, such as algal cells, for expressing a protein encoded by the gene; and methods for stably transforming eukaryotic algae such as Tetraselmis with transgenes.Type: GrantFiled: December 4, 2012Date of Patent: November 11, 2014Assignee: ExxonMobil Research and Engineering CompanyInventors: Jane C. Schneider, Soyan Lieberman, Bo Liu, Eric R. Moellering, John H. Verruto, Amanda Skaggs
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Patent number: 8883467Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.Type: GrantFiled: September 27, 2013Date of Patent: November 11, 2014Assignee: Codexis, Inc.Inventors: Robert McDaniel, Behnaz Behrouzian, Louis Clark, Douglas Hattendorf, Fernando Valle
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Patent number: 8871997Abstract: Disclosed are a variety of methods for achieving enhanced expression from a target nucleotide sequence in a plant e.g. comprising the step of transiently introducing into a tissue of a plant (e.g. a leaf) a first nucleic acid comprising the target nucleotide sequence and a second nucleic acid encoding a Post Transcriptional Gene Silencing (PTGS) suppressor protein (preferably of viral or plant origin), wherein the first and second nucleic acids are comprised within a single binary vector, construct, or the first and second nucleic acid sequences are comprised within a first binary vector and a second binary vector construct respectively. The plant tissue may then be harvested for the protein. Such methods can give much higher levels of gene expression than are obtainable using stable transgenes, or certain replicating vectors.Type: GrantFiled: November 20, 2006Date of Patent: October 28, 2014Assignee: Plant Bioscience LimitedInventors: David Charles Baulcombe, Olivier Voinnet, Andrew John Hamilton