Using A Micro-organism To Make A Protein Or Polypeptide Patents (Class 435/71.1)
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Patent number: 8501448Abstract: The present invention relates to xyloglucanases belonging to family 44 of glycosyl hydrolases and having a relative xyloglucanase activity of at least 30% between pH 5 and pH 8 are derived from the genus Paenibacillus, especially from a strain of Paenibacillus polymyxa or Paenibacillus sp. The xyloglucanases exhibit high performance in conventional detergent compositions.Type: GrantFiled: December 12, 2012Date of Patent: August 6, 2013Assignee: Novozymes A/SInventors: Kirk Schnorr, Martin Schulein, Per Lina Jorgensen
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Patent number: 8501438Abstract: A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of an inhibitor of Pmt-mediated O-linked glycosylation and/or in the presence of one or more ?-1,2-mannosidases.Type: GrantFiled: May 31, 2012Date of Patent: August 6, 2013Assignee: GlycoFi, Inc.Inventors: Piotr Bobrowicz, W. James Cook, Warren Kett
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Patent number: 8501464Abstract: It is described a bidirectional promoter for expression of at least two coding sequences in opposite direction in animal cells; bidirectional expression cassettes; expression constructs; gene transfer expression vectors, and methods for the use thereof.Type: GrantFiled: April 21, 2004Date of Patent: August 6, 2013Assignee: Ospedale San Raffaele S.r.l.Inventors: Luigi Naldini, Mario Amendola, Elisa Vigna
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Patent number: 8501913Abstract: Described are silk proteins derived from spider mite, more specifically derived from Tetranychus urticae. More specifically, described is the use of these proteins to make fibers, or fiber-composed material and the resulting fibers and materials.Type: GrantFiled: October 1, 2010Date of Patent: August 6, 2013Assignees: VIB VZW, Universiteit Gent, The University of Western OntarioInventors: Miodrag Grbic, Vojislava Grbic, Stephane Rombauts, Yves Van De Peer
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Patent number: 8501449Abstract: The present invention relates to methods for the manufacture, purification, formulation, and use of biologically active recombinant elastase proteins. Described are recombinant methods for producing therapeutically useful elastase proteins, as are pharmaceutical compositions comprising said elastase proteins. Novel recombinant elastase proteins and protein preparations are also disclosed. Methods are described for treating and preventing diseases of biological conduits using pharmaceutical compositions containing the elastase proteins of the invention.Type: GrantFiled: December 3, 2008Date of Patent: August 6, 2013Assignee: Proteon Therapeutics, Inc.Inventors: F. Nicholas Franano, Kimberly S. Bland, Marco D. Wong, Bee C. Ding
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Patent number: 8501440Abstract: A fibrillation-resistant insulin analogue may be a single-chain insulin analogue or a physiologically acceptable salt thereof, containing an insulin A chain sequence or an analogue thereof and an insulin B chain sequence or an analogue thereof connected by a polypeptide of 4-10 amino acids. The fibrillation-resistant insulin analogue preferably displays less than 1 percent fibrillation with incubation at 37° C. for at least 21 days. A single-chain insulin analogue displays greater in vitro insulin receptor binding than normal insulin while displaying less than or equal binding to IGFR than normal insulin. The fibrillation-resistant insulin may be used to treat a patient using an implantable or external insulin pump, due to its greater fibrillation resistance.Type: GrantFiled: May 16, 2012Date of Patent: August 6, 2013Assignee: Case Western Reserve UniversityInventor: Michael Weiss
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Publication number: 20130189734Abstract: The present invention provides isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: ApplicationFiled: November 2, 2011Publication date: July 25, 2013Applicant: NOVOZYMES INC.Inventors: Ye Liu, Lan Tang, Junxin Duan
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Patent number: 8492120Abstract: The present invention discloses an improved process for the production of G-CSF in high yield via a high salt-induced increase in plasmid stability during the production phase.Type: GrantFiled: November 23, 2010Date of Patent: July 23, 2013Assignee: Cadila Healthcare LimitedInventors: Sanjeev Kumar Mendiratta, Vibhor Saraswat, Pankaj R. Patel
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Patent number: 8486663Abstract: The compositions and methods of the present invention comprise the efficient and effective presentation of antigens to the appropriate components of the immune system resulting in the production of species-specific antibodies in vitro. In general, these compositions comprise one or more antigenic components together with a colloidal metal, optionally combined with derivatized PEG (polyethylene glycol) or other agents. The invention also comprises methods and compositions for making such colloidal metal compositions.Type: GrantFiled: May 12, 2011Date of Patent: July 16, 2013Assignee: Cytlmmune Sciences, Inc.Inventors: Giulio F. Paciotti, Ramadevi Raghunandan, Marja S. Huhta, Lawrence Tamarkin
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Patent number: 8486678Abstract: The invention describes novel pharmaceutical compositions for the treatment of virus infections and cancer. The pharmaceutical compositions include mutant oligoadenylate synthetases (OAS) that have either enhanced cell permeability, reduced oxidative potential, improved antiviral activity, improved enzymatic activity, or absent enzymatic activity. The pharmaceutical compositions have improved drug properties and retain or have enhanced antiviral activity relative to their native forms. The pharmaceutical compositions further include chemically modified oligoadenylate synthetases, such chemical modifications being designed to increase serum stability and reduce immunogenicity in vivo. Such chemical modifications further increase drug stability and manufacturability in vitro. Compositions composed of more than ninety novel modifications are described. Also described are antibodies to polypeptides of the invention.Type: GrantFiled: February 1, 2012Date of Patent: July 16, 2013Assignee: Kineta Two, LLCInventors: Shawn P Iadonato, Charles L Magness, Mark Branum, Maralee McVean, Christina Scherer
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Patent number: 8486667Abstract: The present invention provides a novel endoglucanase nucleic acid sequence, designated egl7, and the corresponding EGVII amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding EGVII, recombinant EGVII proteins and methods for producing the same.Type: GrantFiled: November 1, 2011Date of Patent: July 16, 2013Assignee: Danisco US Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Patent number: 8481706Abstract: The use of monokaryotic strains of filamentous fungi of the Pycnoporus species of the basidiomycete group, for implementing a method for preparing a specific recombinant protein, the method being carried out by overexpressing the gene encoding the protein in the Pycnoporus monokaryotic strain.Type: GrantFiled: January 14, 2005Date of Patent: July 9, 2013Assignees: Institut National de la Recherche Agronomique, Universite de ProvenceInventors: Alexandra M. C. R. Alves, Eric Record, Anne Lomascolo, Jean-Claude Sigoillot, Marcel Asther, Han A. B. Wösten
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Patent number: 8481321Abstract: The present invention relates to the identification of novel nucleic acid sequences, designated herein as 7p, 8k, 7E, 9G, 8Q and 203, in a host cell which effect protein production. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding 7p, 8k, 7E, 9G, 8Q and 203, which are presented in FIG. 1, and are SEQ ID NOS.: 1-6, respectively. The present invention also provides host cells further comprising a nucleic acid encoding a desired heterologous protein such as an enzyme.Type: GrantFiled: June 12, 2012Date of Patent: July 9, 2013Assignee: Danisco US Inc.Inventors: Elizabeth A. Bodie, Steve Sungjin Kim
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Publication number: 20130164766Abstract: Methods for obtaining highly pure native, recombinant or modified BAD-1 protein include the steps of culturing a population of microbes expressing BAD-1 protein in a culture medium, collecting the population of microbes from the culture medium, obtaining a BAD-1 protein-containing solution, and purifying the BAD-1 protein from the solution by combining the BAD-1 protein-containing solution with a nickel-chelating resin, washing the nickel-chelating resin to remove unbound matter, and eluting the BAD-1 protein from the nickel-chelating resin. Highly pure native BAD-1 protein may be used in diagnostic kits for detecting Blastomyces dermatitidis infections in animals.Type: ApplicationFiled: December 19, 2012Publication date: June 27, 2013Inventors: Bruce Klein, Theodore Brandhorst
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Publication number: 20130164787Abstract: Disclosed herein are cell culture compositions, for example, pancreatic cell culture compositions, derived from dedifferentiated human reprogrammed pluripotent stem cells, such as induced pluripotent stem (iPS) cells, and methods for producing and using such cell culture compositions.Type: ApplicationFiled: February 6, 2013Publication date: June 27, 2013Applicant: VIACYTE, INC.Inventor: ViaCyte, Inc.
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Patent number: 8470576Abstract: The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitriles.Type: GrantFiled: April 5, 2007Date of Patent: June 25, 2013Assignee: B.R.A.I.N. AGInventors: Jürgen Eck, Klaus Liebeton, Lutz Fischer, Sabine Lutz-Wahl, Martina Hensel, Christian Ewert, Marcus Wälz
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Patent number: 8470973Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.Type: GrantFiled: August 25, 2011Date of Patent: June 25, 2013Inventors: Ulla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
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Publication number: 20130157314Abstract: The present invention relates to compositions comprising: a polypeptide having cellulolytic enhancing activity and a heterocyclic compound. The present invention also relates to methods of using the compositions.Type: ApplicationFiled: August 5, 2011Publication date: June 20, 2013Applicant: NOVOZYMES, INC.Inventors: Feng Xu, Matthew Sweeney, Jason Quinlan
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Publication number: 20130156801Abstract: The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest; isolating exosomes from the exosome-producing cell culture; and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.Type: ApplicationFiled: December 15, 2011Publication date: June 20, 2013Applicant: MOREHOUSE SCHOOL OF MEDICINEInventors: Vincent Craig BOND, Michael POWELL, Ming Bo HUANG, Syed ALI
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Patent number: 8465942Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.Type: GrantFiled: June 19, 2012Date of Patent: June 18, 2013Assignee: Bunge Oils, Inc.Inventors: Christopher L. G. Dayton, Nelson Barton, Analia Bueno, Joslin G. Cuenca, Tim Hitchman, Katie A. Kline, Jonathan Lyon, Mark L. Miller, Mark A. Wall
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Publication number: 20130150554Abstract: The present invention provides improved cell culture systems that allow optimum production of recombinant proteins. Among other things, the present invention provides methods of cell culture including a step of cultivating cells adapted to growth factor-free medium in a cell culture system that provides at least one growth factor.Type: ApplicationFiled: August 8, 2011Publication date: June 13, 2013Applicant: WYETH LLCInventors: Mark Wallace Melville, Tara Ann Chamberlain, Martin Sinacore
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Patent number: 8460897Abstract: The fungus Irpex lacteus is efficiently cultured on a solid substrate with a high content of crystalline cellulose. The fungal culture can be used to produce cellulases that are useful in conversion of cellulose to sugars. The fungal culture can also be used to produce chitin, which is itself valuable and can also be converted to chitosan. Use of the fungal culture for co-production of cellulases and chitin is also described.Type: GrantFiled: December 16, 2010Date of Patent: June 11, 2013Assignee: Eclipse Bioproducts, LLCInventors: Eugene N. Bernat, David Scott Hibbett, Dimitrios Floudas
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Patent number: 8455233Abstract: The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.Type: GrantFiled: December 17, 2008Date of Patent: June 4, 2013Assignee: Novozymes A/SInventors: Paul Harris, Suchindra Maiyuran, Kimberly Brown
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Patent number: 8455223Abstract: The present invention relates to proteins having an enzymatic activity of reducing substituted alkanones such as 3-methylamino-1-(2-thienyl)-propan-1-one. The invention furthermore relates to nucleic acids coding for said proteins, nucleic acid constructs, vectors, genetically modified microorganisms and to methods for preparing optically active substituted alkanols, such as, for example, (S)-3-methylamino-1-(2-thienyl)-(S)-propanol.Type: GrantFiled: September 14, 2006Date of Patent: June 4, 2013Assignee: BASF SEInventors: Michael Breuer, Thomas Friedrich, Maria Kesseler
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Patent number: 8445234Abstract: Disclosed herein are methods of making a VEGF-D polypeptide comprising the steps of culturing a host cell transformed or transfected with a vector comprising a nucleic acid sequence encoding the VEGF-D polypeptide operably associated with a promoter sequence such that the nucleic acid sequence encoding the VEGF-D polypeptide is expressed; and isolating the VEGF-D polypeptide from the host cell or from the growth medium in which said host cell is cultured.Type: GrantFiled: May 17, 2012Date of Patent: May 21, 2013Assignee: Vengenics Pty LimitedInventors: Marc G. Achen, Stephen A. Stacker, Kari Alitalo, Andrew F. Wilks
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Publication number: 20130122598Abstract: The invention relates to a process for the specific isotopic labeling of Valine, Leucine and Isoleucine amino acids. The process of the invention uses a 2-alkyl-2-hydroxy-3-oxobutanoic acid in which the alkyl substituent in position 2 is ethyl or methyl. The invention can be used for the analysis of proteins, in particular by NMR.Type: ApplicationFiled: January 6, 2010Publication date: May 16, 2013Applicants: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES, UNIVERSITE JOSEPH FOURIER, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Pierre Gans, Jerome Boisbouvier, Isabel Ayala, Olivier Hamelin
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Patent number: 8440451Abstract: Yeast cells are transformed with an exogenous xylose isomerase gene. Additional genetic modifications enhance the ability of the transformed cells to ferment xylose to ethanol or other desired fermentation products. Those modifications include deletion of non-specific or specific aldose reductase gene(s), deletion of xylitol dehydrogenase gene(s) and/or overexpression of xylulokinase.Type: GrantFiled: May 14, 2011Date of Patent: May 14, 2013Assignee: Cargill, IncorporatedInventors: Vineet Rajgarhia, Kari Koivuranta, Merja Penttilä, Marja Ilmen, Pirkko Suominen, Aristos Aristidou, Christopher Kenneth Miller, Stacey Olson, Laura Ruohonen
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Patent number: 8431360Abstract: The present disclosure relates to host cells containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell. The present disclosure further relates to methods of increasing transport of cellodextrin into a cell, methods of increasing growth of a cell on a medium containing cellodextrin, methods of co-fermenting cellulose-derived and hemicellulose-derived sugars, and methods of making hydrocarbons or hydrocarbon derivatives by providing a host cell containing a recombinant polynucleotide encoding a polypeptide where the polypeptide transports cellodextrin into the cell.Type: GrantFiled: July 26, 2010Date of Patent: April 30, 2013Assignees: The Regents of the University of California, The Board of Trustees of the University of Illinois, BP Corporation North America Inc.Inventors: N. Louise Glass, Chaoguang Tian, William T. Beeson, IV, Huimin Zhao, Jing Du, Jin Ho Choi, James H. Doudna Cate, Jonathan M. Galazka, Suk-Jin Ha, Yong-Su Jin, Soo Rin Kim, Sijin Li, Xiaomin Yang
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Patent number: 8431381Abstract: The present invention provides novel genes for ATP:citrate lyase. A nucleic acid comprising the nucleotide sequence shown in SEQ ID NO: 5, 6, 9 or 10 or a fragment thereof.Type: GrantFiled: October 24, 2008Date of Patent: April 30, 2013Assignee: Suntory Holdings LimitedInventor: Misa Ochiai
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Patent number: 8431364Abstract: Provided are a folyl extract of fermented soybean (EFS) produced by fermenting a culture including a folic acid and soybean extract by using a microorganism, and a composition including the folyl EFS. The folyl EFS has an anti-histamine effect, an anti-allergic effect, a calcium-absorption-promotion effect, a bone-growth-promotion effect, a cell growth promotion effect, a collagen biosynthesis promotion effect, a wrinkle improvement effect, and an UV-induced cell damage inhibition effect. Accordingly, the folyl EFS can be used in a skin external application or cosmetic composition, a health supplement food composition, a feed composition, and a pharmaceutical composition.Type: GrantFiled: October 2, 2008Date of Patent: April 30, 2013Assignee: Damy Chemical Co. Ltd.Inventors: Boo Sun Cho, Kwang Nyeon Kim
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Publication number: 20130102033Abstract: The present invention relates to a culture method for enhancing the production of the extracellular polysaccharopeptides (EPS) by submerged fermentations with a mycelium immobilization by a local Trametes versicolor strain LH1 and significantly increasing the production of polysaccharopeptides with immunomodulatory activity of Trametes versicolor LH1 through mycelium immobilization by liquid-surface complex carrier with gelling material at optimal condition. This method is beneficial to mycelium re-use in fed-batch submerged fermentation by providing a better micro-environment and simplifying downstream processes of isolating and recycling the EPS from broth.Type: ApplicationFiled: October 19, 2011Publication date: April 25, 2013Inventors: Tai-Hao HSU, Fang-Yi Lin
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Publication number: 20130102029Abstract: Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation.Type: ApplicationFiled: October 22, 2012Publication date: April 25, 2013Applicant: XYLECO, INC.Inventor: Xyleco, Inc.
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Patent number: 8426171Abstract: The present invention relates to a method for producing L-methionine and organic acid comprising the following steps: Step 1) preparing a strain producing L-methionine precursor and producing L-methionine precursor by the fermentation of the strain; Step 2) producing L-methionine and organic acid by the enzyme reaction process with the L-methionine precursor as a substrate, and microorganism strains used in each step.Type: GrantFiled: July 30, 2007Date of Patent: April 23, 2013Assignee: CJ Cheiljedang CorporationInventors: So-young Kim, Kwang-myung Cho, Yong-uk Shin, Hye-won Um, Kyung-oh Choi, Jin-sook Chang, Young-wook Cho, Young-hoon Park
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Patent number: 8426167Abstract: The present invention relates to the cloning and expression of foreign protein or polypeptides in bacteria, such as Escherichia coli. In particular, this invention relates to expression tools comprising a FKBP-type peptidyl prolyl isomerase selected from the group consisting of FkpA, SlyD, and trigger factor; methods of recombinant protein expression, the recombinant polypeptides thus obtained, as well as to the use of such polypeptides.Type: GrantFiled: April 13, 2007Date of Patent: April 23, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Christian Scholz, Herbert Andres, Elke Faatz, Alfred Engel, Urban Schmitt, Ariuna Bazarsuren, Peter Schaarschmidt
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Patent number: 8420342Abstract: Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.Type: GrantFiled: April 8, 2011Date of Patent: April 16, 2013Assignee: Bunge Oils, Inc.Inventors: Christopher L. G. Dayton, Tim Hitchman, Katie Kline, Jonathan Lyon, Mark A. Wall, Nelson R. Barton
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Patent number: 8420782Abstract: The present invention refers to methods for selectively recognizing a base pair in a DNA sequence by a polypeptide, to modified polypeptides which specifically recognize one or more base pairs in a DNA sequence and, to DNA which is modified so that it can be specifically recognized by a polypeptide and to uses of the polypeptide and DNA in specific DNA targeting as well as to methods of modulating expression of target genes in a cell.Type: GrantFiled: January 31, 2012Date of Patent: April 16, 2013Inventors: Ulla Bonas, Jens Boch, Sebastian Schornack, Thomas Lahaye
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Patent number: 8415127Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.Type: GrantFiled: November 7, 2011Date of Patent: April 9, 2013Assignee: Codexis, Inc.Inventors: Charlene Ching, John M. Gruber, Gjalt W. Huisman, Emily Mundorff, Lisa M. Newman
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Patent number: 8414903Abstract: The present invention provides methods and compositions for production of gram-negative bacterial mutants that are defective in intestinal colonization capacity and sensitive to infection by bacteriophage P1. Thus the present invention provides immunogenic compositions for the prevention or attenuation of food- and water-borne illnesses associated with ingestion of bacteria such as enterohemorrhagic Escherichia coli.Type: GrantFiled: November 20, 2007Date of Patent: April 9, 2013Assignee: Tufts UniversityInventors: Matthew K. Waldor, Theresa D. Ho
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Patent number: 8415120Abstract: An evolvable production strain of B. subtilis exhibiting continuous or high level expression during protein evolution is described. An evolved Bacillus subtilis pstS promoter facilitates screening and production of secreted proteins.Type: GrantFiled: November 19, 2010Date of Patent: April 9, 2013Assignee: Danisco US Inc.Inventors: Katherine D. Collier, Edwin Lee, Nicholas Leiva, Thomas B. Morrison, Volker Schellenberger, Olga V. Selifonova, Adam C. Kean
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Patent number: 8409838Abstract: An isolated nucleic acid encoding an FX protein having a serine at position 79, a lysine at position 90, a leucine at position 136, an arginine at position 211, a serine at position 289, and a combination thereof is provided. Cells having a gene encoding a modified FX protein are provided, wherein the cells exhibit a reduced ability to fucosylate a glycoprotein at a first temperature, but exhibit the ability to fucosylate the glycoprotein at a second temperature. Methods and compositions for making glycoproteins with reduced fucosylation are provided.Type: GrantFiled: June 1, 2010Date of Patent: April 2, 2013Assignee: Regeneron Pharmaceuticals, Inc.Inventors: Gang Chen, Darya Burakov, James P. Fandl
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Patent number: 8409828Abstract: Chromatographic processes and systems for purifying a botulinum toxin from an APF fermentation medium.Type: GrantFiled: April 7, 2008Date of Patent: April 2, 2013Assignee: Allergan, Inc.Inventors: Hui Xiang, Mingjiang Luo, Ping Wang, Stephen Donovan
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Patent number: 8409642Abstract: Ferulate esterase producing bacterial strains or functional mutants thereof and methods of using ferulate esterase producing bacterial strains as forage additives are disclosed.Type: GrantFiled: September 18, 2008Date of Patent: April 2, 2013Assignee: Pioneer Hi-Bred International, Inc.Inventors: Victor Nsereko, William Rutherford, Brenda Smiley, Annette Spielbauer
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Patent number: 8404471Abstract: Isolated glycyl-tRNA synthetase polypeptides and polynucleotides having non-canonical biological activities are provided, as well as compositions and methods related thereto.Type: GrantFiled: June 26, 2009Date of Patent: March 26, 2013Assignee: aTyr Pharma, Inc.Inventors: Leslie Ann Greene, Ryan Andrew Adams, Fei Hong, Ji Zhao, Eva Rebecka Stephanie Armour, Kristi Helen Piehl
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Patent number: 8399220Abstract: The present invention relates to oral antibacterial compositions comprising trihydroxybenzoate derivatives, e.g., useful for the treatment of gum diseases (e.g., gingivitis or periodontitis) and to methods of using such compositions.Type: GrantFiled: April 16, 2010Date of Patent: March 19, 2013Assignee: Forsyth Dental Infirmary for ChildrenInventors: Toshihisa Kawai, Philip P. Stashenko, Yoshiaka Hosokawa, Kazuhisa Ohara
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Patent number: 8398995Abstract: A method for inducing immune tolerance to type I diabetes in a mammal by mucosal delivery of an antigen involved in the induction of type I diabetes such as pro-insulin or insulin is disclosed. The antigen involved in the induction of type I diabetes is delivered together with an IL-10 secreting micro-organism such as Lactococcus lactis and a anti-CD3 antibody.Type: GrantFiled: October 9, 2009Date of Patent: March 19, 2013Assignee: Actogenix NVInventors: Pieter Rottiers, Veerle Snoeck
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Patent number: 8399651Abstract: The invention provides mutants of GAS57 (Spy0416) which are unable to cleave IL-8 and similar substrates but which still maintain the ability to induce protection against S. pyogenes. The invention also provides antibodies which specifically bind to GAS57 and which inhibit its ability to cleave IL-8 and similar substrates. The mutants are useful, inter alia, in vaccine compositions to induce protection against S. pyogenes. The antibodies are useful, e.g., as therapeutics for treating S. pyogenes infections.Type: GrantFiled: September 10, 2012Date of Patent: March 19, 2013Assignee: Novartis AGInventors: Immaculada Margarit Y Ros, Guido Grandi, Chiara Zingaretti
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Patent number: 8399232Abstract: An object of the present invention is to provide enzymes associated with equol synthesis, genes coding such enzymes, and a process for producing equol and its intermediates using the enzymes and genes. The present invention provides a dihydrodaidzein synthesizing enzyme, tetrahydrodaidzein synthesizing enzyme, equol synthesizing enzyme, and genes coding these enzymes. The present invention also provides a process for synthesizing dihydrodaidzein, tetrahydrodaidzein, and/or equol using these enzymes.Type: GrantFiled: December 25, 2008Date of Patent: March 19, 2013Assignee: Otsuka Pharmaceutical Co., Ltd.Inventors: Yoshikazu Shimada, Setsuko Yasuda, Masayuki Takahashi, Takashi Hayashi, Norihiro Miyazawa, Yasuhiro Abiru, Tadaaki Ohtani, Ikutaro Sato
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Publication number: 20130065810Abstract: The present invention is related to a fungal serine protease enzyme, which comprises an amino acid sequence of the mature Fe_RF6318 enzyme having an amino acid sequence of SEQ ID NO: 15. The serine protease is obtainable from Fusarium equiseti, more preferably from the deposited strain CBS 119568. Also disclosed are nucleic acid sequences encoding said protease, such as plasmid pALK2521 comprising the nucleotide sequence SEQ ID NO:9 deposited in E. coli RF7664 under accession number DSM 22171 and plasmid pALK2529 comprising the full-length gene SEQ ID NO: 10 deposited in E. coli RF7800 under accession number DSM 22172. Said protease is useful as an enzyme preparation applicable in detergent compositions and for treating fibers, for treating wool, for treating hair, for treating leather, for treating food or feed, or for any applications involving modification, degradation or removal of proteinaceous material.Type: ApplicationFiled: April 28, 2010Publication date: March 14, 2013Applicant: AB Enzymes OyInventors: Kari Juntunen, Leena Valtakari, Susanna Makinen, Jarno Kallio, Jari Vehmaanpera, Pentti Ojapalo, Marja Paloheimo
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Publication number: 20130065270Abstract: The present invention relates to methods for producing Saccharomyces strains that are capable of growth on xylose as a sole carbon source at a desired growth rate, (such as at least one generation per 48 hours), strains made by such methods, and Saccharomyces strains that grow at a growth rate of at least one generation per 48 hours using xylose as a sole carbon source for growth made by non-recombinant methods.Type: ApplicationFiled: August 20, 2012Publication date: March 14, 2013Applicant: Microbiogen Pty Ltd.Inventors: Philip John Livingstone Bell, Paul Victor Attfield
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Publication number: 20130059332Abstract: The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution.Type: ApplicationFiled: September 14, 2012Publication date: March 7, 2013Applicant: BASF SEInventors: Matthias Boy, Jong-Kyu Choi, Jin Won Chung, Markus Lohscheidt, Jong In Choi, Jae Yeol Seo, Jörg Braun, Mo Se Kim, Sung Hyun Kim, Arno Kochner